Aprt cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aprt
Entrez Gene ID	11821
Full Name	adenine phosphoribosyl transferase
Synonyms	C85684
General protein information	Preferred Names adenine phosphoribosyl transferase Names adenine phosphoribosyltransferase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 E1\|8 71.91 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_009698.2	NP_033828.2	adenine phosphoribosyltransferase

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AER325W	NP_985181.1
Magnaporthe oryzae (rice blast fungus)	MGG_17399	XP_003718359.1
Canis lupus familiaris (dog)	APRT	XP_536752.2
Mus musculus (house mouse)	Aprt	NP_033828.2
Danio rerio (zebrafish)	aprt	NP_956962.1
Saccharomyces cerevisiae (baker's yeast)	APT1	NP_013690.1
Neurospora crassa	NCU02090	XP_963727.2
Xenopus tropicalis (tropical clawed frog)	aprt	NP_001007941.1
Homo sapiens (human)	APRT	NP_000476.1
	APRT	XP_001089867.1
Bos taurus (cattle)	APRT	NP_001020505.1
Gallus gallus (chicken)	APRT	NP_001264576.1
Drosophila melanogaster (fruit fly)	Aprt	NP_476637.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005723	XP_315739.4
Kluyveromyces lactis	KLLA0B01309g	XP_451589.1
Schizosaccharomyces pombe (fission yeast)	apt1	NP_594433.1
Oryza sativa (rice)	Os12g0589100	NP_001067164.1
Pan troglodytes (chimpanzee)	APRT	XP_003952966.1
Rattus norvegicus (Norway rat)	Aprt	NP_001013079.1
Caenorhabditis elegans (roundworm)	T19B4.3	NP_491663.1
Arabidopsis thaliana (thale cress)	APT1	NP_564284.1

Gene Ontology
Bibliography

Related articles in PubMed

Simulated space radiation-induced mutants in the mouse kidney display widespread genomic change.
Turker MS, Grygoryev D, Lasarev M, Ohlrich A, Rwatambuga FA, Johnson S, Dan C, Eckelmann B, Hryciw G, Mao JH, Snijders AM, Gauny S, Kronenberg A
PloS one12(7)e0180412(2017)

SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair.
Vazquez BN, Thackray JK, Simonet NG, Kane-Goldsmith N, Martinez-Redondo P, Nguyen T, Bunting S, Vaquero A, Tischfield JA, Serrano L
The EMBO journal35(14)1488-503(2016 07)

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Lewandowski JP, Du F, Zhang S, Powell MB, Falkenstein KN, Ji H, Vokes SA
Developmental biology406(1)92-103(2015 Oct)

Prdx1 deficiency in mice promotes tissue specific loss of heterozygosity mediated by deficiency in DNA repair and increased oxidative stress.
Rani V, Neumann CA, Shao C, Tischfield JA
Mutation research735(1-2)39-45(2012 Jul)

Marked aneuploidy and loss of multiple chromosomes are common in autosomal mutants isolated from normal mouse kidney epithelium.
Dan C, Grygoryev D, Sandfort K, Connolly M, Cross B, Lasarev M, Kronenberg A, Turker MS
Genes, chromosomes & cancer50(4)239-49(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LOH on nonselected chromosomes was infrequent in Aprt mutants exhibiting intragenic events or mitotic recombination for chromosome 8
Title: Marked aneuploidy and loss of multiple chromosomes are common in autosomal mutants isolated from normal mouse kidney epithelium.

Data shsow that despite continuous selection for expression of the reactivated Aprt alleles, exceptionally high spontaneous re-silencing frequencies were observed.
Title: Aberrantly silenced promoters retain a persistent memory of the silenced state after long-term reactivation.

silencing of mouse Aprt is a gradual process in the differentiated cells
Title: Silencing of mouse Aprt is a gradual process in differentiated cells.

The following Aprt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aprt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04679	NM_009698.2 Latest version!	Mus musculus adenine phosphoribosyl transferase (Aprt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aprt ( NM_009698.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033828.2 Mus musculus adenine phosphoribosyl transferase (Aprt), mRNA.

Price & Availability↑

CloneID	OMu04679
Clone ID Related Accession (Same CDS sequence)	NM_009698.2
Accession Version	NM_009698.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Mus musculus(house mouse)
Product	adenine phosphoribosyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY115322.1 and AK153201.1. On Dec 2, 2006 this sequence version replaced NM_009698.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153201.1, CK790193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGAAC CTGAGTTGAA ACTGGTGGCG CGGCGCATCC GCAGCTTCCC CGACTTCCCA 
ATCCCGGGCG TGCTGTTCAG GGATATCTCG CCCCTCTTGA AAGACCCGGA CTCCTTCCGA 
GCTTCCATCC GCCTCTTGGC CAGTCACCTG AAGTCCACGC ACAGCGGCAA GATCGACTAC 
ATCGCAGGTC TAGACTCCAG GGGCTTCCTG TTTGGCCCTT CCCTAGCTCA GGAGCTGGGC 
GTGGGCTGTG TGCTCATCCG GAAACAGGGG AAGCTGCCGG GCCCCACTGT GTCAGCCTCC 
TATTCTCTGG AGTATGGGAA GGCTGAGCTG GAAATCCAGA AAGATGCCTT GGAACCCGGG 
CAGAGAGTGG TCATTGTGGA TGACCTCCTG GCCACAGGAG GAACCATGTT TGCGGCCTGT 
GACCTGCTGC ACCAGCTCCG GGCTGAAGTG GTGGAGTGTG TGAGCCTGGT GGAGCTGACC 
TCGCTGAAGG GCAGGGAGAG GCTAGGACCT ATACCATTCT TCTCTCTCCT CCAGTATGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033828.2
CDS	76..618
Translation	MSEPELKLVARRIRSFPDFPIPGVLFRDISPLLKDPDSFRASIRLLASHLKSTHSGKIDYIAGLDSRGFLFGPSLAQELGVGCVLIRKQGKLPGPTVSASYSLEYGKAELEIQKDALEPGQRVVIVDDLLATGGTMFAACDLLHQLRAEVVECVSLVELTSLKGRERLGPIPFFSLLQYD

Target ORF information:

RefSeq Version	NM_009698.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus adenine phosphoribosyl transferase (Aprt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009698.2

ORF Insert Sequence:

ATGTCGGAAC CTGAGTTGAA ACTGGTGGCG CGGCGCATCC GCAGCTTCCC CGACTTCCCA 
ATCCCGGGCG TGCTGTTCAG GGATATCTCG CCCCTCTTGA AAGACCCGGA CTCCTTCCGA 
GCTTCCATCC GCCTCTTGGC CAGTCACCTG AAGTCCACGC ACAGCGGCAA GATCGACTAC 
ATCGCAGGTC TAGACTCCAG GGGCTTCCTG TTTGGCCCTT CCCTAGCTCA GGAGCTGGGC 
GTGGGCTGTG TGCTCATCCG GAAACAGGGG AAGCTGCCGG GCCCCACTGT GTCAGCCTCC 
TATTCTCTGG AGTATGGGAA GGCTGAGCTG GAAATCCAGA AAGATGCCTT GGAACCCGGG 
CAGAGAGTGG TCATTGTGGA TGACCTCCTG GCCACAGGAG GAACCATGTT TGCGGCCTGT 
GACCTGCTGC ACCAGCTCCG GGCTGAAGTG GTGGAGTGTG TGAGCCTGGT GGAGCTGACC 
TCGCTGAAGG GCAGGGAGAG GCTAGGACCT ATACCATTCT TCTCTCTCCT CCAGTATGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Simulated space radiation-induced mutants in the mouse kidney display widespread genomic change.
PloS one12(7)e0180412(2017)
Turker MS,Grygoryev D,Lasarev M,Ohlrich A,Rwatambuga FA,Johnson S,Dan C,Eckelmann B,Hryciw G,Mao JH,Snijders AM,Gauny S,Kronenberg A

SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair.
The EMBO journal35(14)1488-503(2016 07)
Vazquez BN,Thackray JK,Simonet NG,Kane-Goldsmith N,Martinez-Redondo P,Nguyen T,Bunting S,Vaquero A,Tischfield JA,Serrano L

Spatiotemporal regulation of GLI target genes in the mammalian limb bud.
Developmental biology406(1)92-103(2015 Oct)
Lewandowski JP,Du F,Zhang S,Powell MB,Falkenstein KN,Ji H,Vokes SA

Prdx1 deficiency in mice promotes tissue specific loss of heterozygosity mediated by deficiency in DNA repair and increased oxidative stress.
Mutation research735(1-2)39-45(2012 Jul)
Rani V,Neumann CA,Shao C,Tischfield JA

Marked aneuploidy and loss of multiple chromosomes are common in autosomal mutants isolated from normal mouse kidney epithelium.
Genes, chromosomes & cancer50(4)239-49(2011 Apr)
Dan C,Grygoryev D,Sandfort K,Connolly M,Cross B,Lasarev M,Kronenberg A,Turker MS