APRT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	APRT
Entrez Gene ID	353
Full Name	adenine phosphoribosyltransferase
Synonyms	AMP,APRTD
General protein information	Preferred Names adenine phosphoribosyltransferase Names adenine phosphoribosyltransferase AMP diphosphorylase AMP pyrophosphorylase transphosphoribosidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q24.3
Summary	Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	102600
Disorder Html:	Adenine phosphoribosyltransferase deficiency, 614723 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000485.2	NP_000476.1	adenine phosphoribosyltransferase isoform a
NM_001030018.2	NP_001025189.1	adenine phosphoribosyltransferase isoform b
NM_000485.3	NP_000476.1	adenine phosphoribosyltransferase isoform a
NM_001030018.1	NP_001025189.1	adenine phosphoribosyltransferase isoform b

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AER325W	NP_985181.1
Magnaporthe oryzae (rice blast fungus)	MGG_17399	XP_003718359.1
Canis lupus familiaris (dog)	APRT	XP_536752.2
Mus musculus (house mouse)	Aprt	NP_033828.2
Danio rerio (zebrafish)	aprt	NP_956962.1
Saccharomyces cerevisiae (baker's yeast)	APT1	NP_013690.1
Neurospora crassa	NCU02090	XP_963727.2
Xenopus tropicalis (tropical clawed frog)	aprt	NP_001007941.1
Homo sapiens (human)	APRT	NP_000476.1
	APRT	XP_001089867.1
Bos taurus (cattle)	APRT	NP_001020505.1
Gallus gallus (chicken)	APRT	NP_001264576.1
Drosophila melanogaster (fruit fly)	Aprt	NP_476637.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005723	XP_315739.4
Kluyveromyces lactis	KLLA0B01309g	XP_451589.1
Schizosaccharomyces pombe (fission yeast)	apt1	NP_594433.1
Oryza sativa (rice)	Os12g0589100	NP_001067164.1
Pan troglodytes (chimpanzee)	APRT	XP_003952966.1
Rattus norvegicus (Norway rat)	Aprt	NP_001013079.1
Caenorhabditis elegans (roundworm)	T19B4.3	NP_491663.1
Arabidopsis thaliana (thale cress)	APT1	NP_564284.1

Gene Ontology
Bibliography

Related articles in PubMed

A thymine-adenine dinucleotide repeat polymorphism near IL28B is associated with spontaneous clearance of hepatitis C virus.
Hiramine S, Sugiyama M, Furusyo N, Uto H, Ido A, Tsubouchi H, Watanabe H, Ueno Y, Korenaga M, Murata K, Masaki N, Hayashi J, Thomas DL, Mizokami M
Journal of gastroenterology50(10)1069-77(2015 Oct)

Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation.
Valaperta R, Rizzo V, Lombardi F, Verdelli C, Piccoli M, Ghiroldi A, Creo P, Colombo A, Valisi M, Margiotta E, Panella R, Costa E
BMC nephrology15102(2014 Jul)

A Japanese boy with adenine phosphoribosyltransferase (APRT) deficiency caused by compound heterozygosity including a novel missense mutation in APRT gene.
Nozue H, Kamoda T, Saitoh H, Ichikawa K, Taniguchi A
Acta paediatrica (Oslo, Norway : 1992)100(12)e285-8(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A long TA repeat in the promoter region of IL28B was associated with spontaneous HCV clearance.
Title: A thymine-adenine dinucleotide repeat polymorphism near IL28B is associated with spontaneous clearance of hepatitis C virus.

A new mutation, p.Gln147X, in APRT gene, was found in a patient with adenine phosphoribosyltransferase deficiency.
Title: Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation.

Case study of 2 year old Japanese boy with APRT deficiency. Genetic analysis showed compound heterozygote APRT*J and missense mutation L33P. APRT deficiency should be suspected in patients with radiolucent kidney stones and urinary 2,8-DHA crystals.
Title: A Japanese boy with adenine phosphoribosyltransferase (APRT) deficiency caused by compound heterozygosity including a novel missense mutation in APRT gene.

The phosphorylation status of membrane-bound nucleoside diphosphate kinase in epithelia and the role of AMP are reported.
Title: The phosphorylation status of membrane-bound nucleoside diphosphate kinase in epithelia and the role of AMP.

Data indicates that the flexible loop structure adopts an open conformation before and after binding of both substrates adenine and phosphoribosyl pyrophosphate.
Title: Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism.

The following APRT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APRT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16247	NM_001030018.2 Latest version!	Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19571	NM_000485.3 Latest version!	Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu19571	NM_000485.2	Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu16247	NM_001030018.1	Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APRT ( NM_001030018.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025189.1 Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16247
Clone ID Related Accession (Same CDS sequence)	NM_001030018.2 , NM_001030018.1
Accession Version	NM_001030018.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	adenine phosphoribosyltransferase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM423481.1 and BM550173.1. On May 31, 2019 this sequence version replaced NM_001030018.1. Transcript Variant: This variant (2) lacks an alternate segment compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.703859.1, SRR7346977.131814.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC 
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC 
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA 
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC 
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA 
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025189.1
CDS	30..434
Translation	MADSELQLVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARHLKATHGGRIDYIAGLDSRGFLFGPSLAQELGLGCVLIRKRGKLPGPTLWASYSLEYGKAELEIQKDALEPGQRVVVVDDLLATGV

Target ORF information:

RefSeq Version	NM_001030018.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030018.2

ORF Insert Sequence:

ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC 
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC 
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA 
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC 
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA 
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APRT ( NM_001030018.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025189.1 Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16247
Clone ID Related Accession (Same CDS sequence)	NM_001030018.2 , NM_001030018.1
Accession Version	NM_001030018.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	adenine phosphoribosyltransferase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM423481.1 and BM550173.1. Transcript Variant: This variant (2) lacks an alternate segment compared to variant 1, that causes a frameshift. The resulting isoform (b) is shorter and has a distinct C-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.239280.1, SRR1163658.470780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144335, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC 
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC 
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA 
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC 
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA 
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025189.1
CDS	36..440
Translation	MADSELQLVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARHLKATHGGRIDYIAGLDSRGFLFGPSLAQELGLGCVLIRKRGKLPGPTLWASYSLEYGKAELEIQKDALEPGQRVVVVDDLLATGV

Target ORF information:

RefSeq Version	NM_001030018.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030018.1

ORF Insert Sequence:

ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC 
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC 
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA 
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC 
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA 
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APRT ( NM_000485.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000476.1 Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19571
Clone ID Related Accession (Same CDS sequence)	NM_000485.2 , NM_000485.3
Accession Version	NM_000485.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	adenine phosphoribosyltransferase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM423481.1 and BU507629.1. This sequence is a reference standard in the RefSeqGene project. On Aug 3, 2005 this sequence version replaced NM_000485.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM562053.1, SRR1163658.328506.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC 
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC 
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA 
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC 
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA 
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT 
GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC 
TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000476.1
CDS	36..578
Translation	MADSELQLVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARHLKATHGGRIDYIAGLDSRGFLFGPSLAQELGLGCVLIRKRGKLPGPTLWASYSLEYGKAELEIQKDALEPGQRVVVVDDLLATGGTMNAACELLGRLQAEVLECVSLVELTSLKGREKLAPVPFFSLLQYE

Target ORF information:

RefSeq Version	NM_000485.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000485.2

ORF Insert Sequence:

ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC 
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC 
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA 
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC 
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA 
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT 
GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC 
TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APRT ( NM_000485.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000476.1 Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19571
Clone ID Related Accession (Same CDS sequence)	NM_000485.2 , NM_000485.3
Accession Version	NM_000485.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	adenine phosphoribosyltransferase isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM423481.1, BU507629.1 and AC092384.5. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_000485.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.627404.1, SRR7410570.627401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378364.8/ ENSP00000367615.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC 
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC 
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA 
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC 
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA 
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT 
GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC 
TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000476.1
CDS	30..572
Translation	MADSELQLVEQRIRSFPDFPTPGVVFRDISPVLKDPASFRAAIGLLARHLKATHGGRIDYIAGLDSRGFLFGPSLAQELGLGCVLIRKRGKLPGPTLWASYSLEYGKAELEIQKDALEPGQRVVVVDDLLATGGTMNAACELLGRLQAEVLECVSLVELTSLKGREKLAPVPFFSLLQYE

Target ORF information:

RefSeq Version	NM_000485.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adenine phosphoribosyltransferase (APRT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000485.3

ORF Insert Sequence:

ATGGCCGACT CCGAGCTGCA GCTGGTTGAG CAGCGGATCC GCAGCTTCCC CGACTTCCCC 
ACCCCAGGCG TGGTATTCAG GGACATCTCG CCCGTCCTGA AGGACCCCGC CTCCTTCCGC 
GCCGCCATCG GCCTCCTGGC GCGACACCTG AAGGCGACCC ACGGGGGCCG CATCGACTAC 
ATCGCAGGCC TAGACTCCCG AGGCTTCCTC TTTGGCCCCT CCCTGGCCCA GGAGCTTGGA 
CTGGGCTGCG TGCTCATCCG AAAGCGGGGG AAGCTGCCAG GCCCCACTCT GTGGGCCTCC 
TATTCCCTGG AGTACGGGAA GGCTGAGCTG GAGATTCAGA AAGACGCCCT GGAGCCAGGA 
CAGAGGGTGG TCGTCGTGGA TGATCTGCTG GCCACTGGTG GAACCATGAA CGCTGCCTGT 
GAGCTGCTGG GCCGCCTGCA GGCTGAGGTC CTGGAGTGCG TGAGCCTGGT GGAGCTGACC 
TCGCTTAAGG GCAGGGAGAA GCTGGCACCT GTACCCTTCT TCTCTCTCCT GCAGTATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A thymine-adenine dinucleotide repeat polymorphism near IL28B is associated with spontaneous clearance of hepatitis C virus.
Journal of gastroenterology50(10)1069-77(2015 Oct)
Hiramine S,Sugiyama M,Furusyo N,Uto H,Ido A,Tsubouchi H,Watanabe H,Ueno Y,Korenaga M,Murata K,Masaki N,Hayashi J,Thomas DL,Mizokami M

Adenine phosphoribosyltransferase (APRT) deficiency: identification of a novel nonsense mutation.
BMC nephrology15102(2014 Jul)
Valaperta R,Rizzo V,Lombardi F,Verdelli C,Piccoli M,Ghiroldi A,Creo P,Colombo A,Valisi M,Margiotta E,Panella R,Costa E

A Japanese boy with adenine phosphoribosyltransferase (APRT) deficiency caused by compound heterozygosity including a novel missense mutation in APRT gene.
Acta paediatrica (Oslo, Norway : 1992)100(12)e285-8(2011 Dec)
Nozue H,Kamoda T,Saitoh H,Ichikawa K,Taniguchi A

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APRT cDNA ORF clone, Homo sapiens(human)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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