Tirap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tirap
Entrez Gene ID	117149
Full Name	toll-interleukin 1 receptor (TIR) domain-containing adaptor protein
Synonyms	AA407980,C130027E04Rik,Mal,Tlr4ap,Wyatt
General protein information	Preferred Names toll-interleukin 1 receptor (TIR) domain-containing adaptor protein Names toll/interleukin-1 receptor domain-containing adapter protein TIR domain-containing adapter protein Toll-like receptor 4 adaptor protein adaptor protein Wyatt myD88 adapter-like protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001177847.1	NP_001171318.1	toll/interleukin-1 receptor domain-containing adapter protein
XM_030244028.1	XP_030099888.1	toll/interleukin-1 receptor domain-containing adapter protein isoform X1
NM_001177845.1	NP_001171316.1	toll/interleukin-1 receptor domain-containing adapter protein
NM_001177846.1	NP_001171317.1	toll/interleukin-1 receptor domain-containing adapter protein
NM_054096.2	NP_473437.2	toll/interleukin-1 receptor domain-containing adapter protein

Pathways from BioSystems

Homologies

	TIRAP	NP_001123907.1
Mus musculus (house mouse)	Tirap	NP_001171316.1
Rattus norvegicus (Norway rat)	Tirap	XP_001055833.2
Gallus gallus (chicken)	TIRAP	NP_001020000.1
Xenopus tropicalis (tropical clawed frog)	LOC100491693	XP_004916474.1
Homo sapiens (human)	TIRAP	NP_001034750.1
Canis lupus familiaris (dog)	TIRAP	XP_851910.3
Pan troglodytes (chimpanzee)	TIRAP	NP_001123933.1
Bos taurus (cattle)	TIRAP	NP_001035051.1
Danio rerio (zebrafish)	tirap	XP_002667158.2
Xenopus tropicalis (tropical clawed frog)	tirap	NP_001037925.1

Gene Ontology
Bibliography

Related articles in PubMed

Heterotrimeric complex of p38 MAPK, PKC?, and TIRAP is required for AP1 mediated inflammatory response.
Baig MS, Liu D, Muthu K, Roy A, Saqib U, Naim A, Faisal SM, Srivastava M, Saluja R
International immunopharmacology48211-218(2017 Jul)

Human Adaptive Immunity Rescues an Inborn Error of Innate Immunity.
Israel L, Wang Y, Bulek K, Della Mina E, Zhang Z, Pedergnana V, Chrabieh M, Lemmens NA, Sancho-Shimizu V, Descatoire M, Lasseau T, Israelsson E, Lorenzo L, Yun L, Belkadi A, Moran A, Weisman LE, Vandenesch F, Batteux F, Weller S, Levin M, Herberg J, Abhyankar A, Prando C, Itan Y, van Wamel WJB, Picard C, Abel L, Chaussabel D, Li X, Beutler B, Arkwright PD, Casanova JL, Puel A
Cell168(5)789-800.e10(2017 Feb)

TIARP attenuates autoantibody-mediated arthritis via the suppression of neutrophil migration by reducing CXCL2/CXCR2 and IL-6 expression.
Inoue A, Matsumoto I, Tanaka Y, Umeda N, Takai C, Kawaguchi H, Ebe H, Yoshida H, Matsumoto Y, Segawa S, Takahashi S, Sumida T
Scientific reports638684(2016 Dec)

MyD88 Adapter-like (Mal)/TIRAP Is Required for Cytokine Production by Splenic Ly6CloTLR2hi but Not by Ly6ChiTLR2hi Monocytes during Trypanosoma cruzi Infection.
Gravina HD, Goes AM, Murta SM, Ropert C
The Journal of biological chemistry291(45)23832-23841(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The adapter Mal (encoded by TIRAP) has appeared crucial for the cytokine production by Ly6C(lo) but not by Ly6C(hi) monocytes. The protein Mal was necessary to induce cytokine synthesis by Ly6C(lo) monocytes after triggering TLR2 or TLR9.
Title: MyD88 Adapter-like (Mal)/TIRAP Is Required for Cytokine Production by Splenic Ly6CloTLR2hi but Not by Ly6ChiTLR2hi Monocytes during Trypanosoma cruzi Infection.

Results suggest that TIR domain-containing adaptor protein positively regulated BV2 microglial M1 polarization through toll-like receptor 4 -mediated Transforming Growth Factor-Beta-Activated Kinase 1/I-Kappa-B Kinase /Nuclear Factor of Kappa Light Polypeptide Gene Enhancer in B-Cells (TAK1/IKK/NF-kappaB), mitogen-activated protein kinases and Akt signalling pathways.
Title: Toll-Interleukin 1 Receptor domain-containing adaptor protein positively regulates BV2 cell M1 polarization.

genetic polymorphism is associated with interferon-gamma signaling and susceptibility to infections
Title: A Common Variant in the Adaptor Mal Regulates Interferon Gamma Signaling.

TIRAP/Mal deficiency significantly inhibited the activity of titanium particles with adherent bacterial debris to stimulate in vivo osteolysis and in vitro cytokine mRNAs and secretion.
Title: Particle-Induced Osteolysis Is Mediated by TIRAP/Mal in Vitro and in Vivo: Dependence on Adherent Pathogen-Associated Molecular Patterns.

The D-helix peptide, 2R9, also potently inhibited TLR4, TLR7, and TLR9, but not TLR3 or TNF-alpha signaling. Cell imaging, co-immunoprecipitation, and in vitro studies demonstrated that 2R9 preferentially targets TIRAP.
Title: A Decoy Peptide that Disrupts TIRAP Recruitment to TLRs Is Protective in a Murine Model of Influenza.

The following Tirap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tirap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22676	NM_001177847.1 Latest version!	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22676	NM_001177845.1 Latest version!	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22676	NM_054096.2 Latest version!	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22676	NM_001177846.1 Latest version!	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22676	XM_030244028.1 Latest version!	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tirap ( NM_001177847.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171318.1 Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu22676
Clone ID Related Accession (Same CDS sequence)	NM_001177847.1 , XM_030244028.1 , NM_001177845.1 , NM_001177846.1 , NM_054096.2
Accession Version	NM_001177847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	toll/interleukin-1 receptor domain-containing adapter protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY743490.1, AK151117.1, AK168494.1, AK081514.1 and BY507516.1. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK151117.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171318.1
CDS	408..1157
Translation	MVSLEACTMASSSSVPASSTPSKKPRDKIADWFRQALLKKPKKMPISQESHLYDGSQTATQDGLSPSSCSSPPSHSSPESRSSPSSCSSGMSPTSPPTHVDSSSSSSGRWSKDYDVCVCHSEEDLEAAQELVSYLEGSQASLRCFLQLRDAAPGGAIVSELCQALSRSHCRVLLITPGFLRDPWCKYQMLQALTEAPASEGCTIPLLSGLSRAAYPPELRFMYYVDGRGKDGGFYQVKEAVIHYLETLS

Target ORF information:

RefSeq Version	NM_001177847.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177847.1

ORF Insert Sequence:

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tirap ( NM_001177845.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171316.1 Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22676
Clone ID Related Accession (Same CDS sequence)	NM_001177847.1 , XM_030244028.1 , NM_001177845.1 , NM_001177846.1 , NM_054096.2
Accession Version	NM_001177845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	toll/interleukin-1 receptor domain-containing adapter protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK080135.1, AK168494.1, AK081514.1 and BY507516.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK080135.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171316.1
CDS	480..1229
Translation	MVSLEACTMASSSSVPASSTPSKKPRDKIADWFRQALLKKPKKMPISQESHLYDGSQTATQDGLSPSSCSSPPSHSSPESRSSPSSCSSGMSPTSPPTHVDSSSSSSGRWSKDYDVCVCHSEEDLEAAQELVSYLEGSQASLRCFLQLRDAAPGGAIVSELCQALSRSHCRVLLITPGFLRDPWCKYQMLQALTEAPASEGCTIPLLSGLSRAAYPPELRFMYYVDGRGKDGGFYQVKEAVIHYLETLS

Target ORF information:

RefSeq Version	NM_001177845.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177845.1

ORF Insert Sequence:

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tirap ( NM_001177846.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001171317.1 Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu22676
Clone ID Related Accession (Same CDS sequence)	NM_001177847.1 , XM_030244028.1 , NM_001177845.1 , NM_001177846.1 , NM_054096.2
Accession Version	NM_001177846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	toll/interleukin-1 receptor domain-containing adapter protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY740695.1, AK036431.1, AK168494.1, AK081514.1 and BY507516.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK036431.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171317.1
CDS	462..1211
Translation	MVSLEACTMASSSSVPASSTPSKKPRDKIADWFRQALLKKPKKMPISQESHLYDGSQTATQDGLSPSSCSSPPSHSSPESRSSPSSCSSGMSPTSPPTHVDSSSSSSGRWSKDYDVCVCHSEEDLEAAQELVSYLEGSQASLRCFLQLRDAAPGGAIVSELCQALSRSHCRVLLITPGFLRDPWCKYQMLQALTEAPASEGCTIPLLSGLSRAAYPPELRFMYYVDGRGKDGGFYQVKEAVIHYLETLS

Target ORF information:

RefSeq Version	NM_001177846.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177846.1

ORF Insert Sequence:

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tirap ( NM_054096.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_473437.2 Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22676
Clone ID Related Accession (Same CDS sequence)	NM_001177847.1 , XM_030244028.1 , NM_001177845.1 , NM_001177846.1 , NM_054096.2
Accession Version	NM_054096.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	toll/interleukin-1 receptor domain-containing adapter protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY740695.1, AF378130.1, AK168494.1, AK081514.1 and BY507516.1. On Apr 29, 2010 this sequence version replaced NM_054096.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF378130.1, AK168494.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_473437.2
CDS	472..1221
Translation	MVSLEACTMASSSSVPASSTPSKKPRDKIADWFRQALLKKPKKMPISQESHLYDGSQTATQDGLSPSSCSSPPSHSSPESRSSPSSCSSGMSPTSPPTHVDSSSSSSGRWSKDYDVCVCHSEEDLEAAQELVSYLEGSQASLRCFLQLRDAAPGGAIVSELCQALSRSHCRVLLITPGFLRDPWCKYQMLQALTEAPASEGCTIPLLSGLSRAAYPPELRFMYYVDGRGKDGGFYQVKEAVIHYLETLS

Target ORF information:

RefSeq Version	NM_054096.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_054096.2

ORF Insert Sequence:

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tirap ( XM_030244028.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030099888.1 Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant X1, mRNA.

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CloneID	OMu22676
Clone ID Related Accession (Same CDS sequence)	NM_001177847.1 , XM_030244028.1 , NM_001177845.1 , NM_001177846.1 , NM_054096.2
Accession Version	XM_030244028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	toll/interleukin-1 receptor domain-containing adapter protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030099888.1
CDS	431..1180
Translation	MVSLEACTMASSSSVPASSTPSKKPRDKIADWFRQALLKKPKKMPISQESHLYDGSQTATQDGLSPSSCSSPPSHSSPESRSSPSSCSSGMSPTSPPTHVDSSSSSSGRWSKDYDVCVCHSEEDLEAAQELVSYLEGSQASLRCFLQLRDAAPGGAIVSELCQALSRSHCRVLLITPGFLRDPWCKYQMLQALTEAPASEGCTIPLLSGLSRAAYPPELRFMYYVDGRGKDGGFYQVKEAVIHYLETLS

Target ORF information:

RefSeq Version	XM_030244028.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus toll-interleukin 1 receptor (TIR) domain-containing adaptor protein (Tirap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030244028.1

ORF Insert Sequence:

ATGGTTAGTT TGGAGGCCTG CACTATGGCT TCATCCTCCT CCGTCCCAGC CTCCTCCACT 
CCGTCCAAGA AGCCTCGAGA CAAGATAGCT GACTGGTTCA GGCAGGCTCT GTTGAAGAAG 
CCCAAGAAGA TGCCGATCTC CCAGGAAAGC CACCTCTATG ATGGTTCACA GACAGCCACA 
CAGGATGGTC TCTCACCCTC GAGCTGCAGC TCACCCCCGA GTCACAGTTC ACCGGAGAGC 
CGTAGCTCAC CCTCGAGCTG CAGTTCAGGA ATGTCACCTA CCTCGCCACC AACACACGTG 
GACAGCAGCA GCAGCAGCAG TGGCCGCTGG AGCAAAGACT ACGATGTCTG CGTGTGCCAC 
AGTGAGGAGG ACTTGGAGGC GGCCCAGGAG CTGGTCTCCT ACTTGGAGGG TAGCCAGGCC 
AGTCTACGCT GCTTCCTGCA GCTTCGGGAT GCAGCCCCGG GTGGCGCCAT TGTTTCGGAG 
CTATGCCAGG CACTGAGTCG TAGTCACTGC CGTGTGCTGC TCATCACTCC AGGCTTCCTT 
CGGGACCCCT GGTGCAAGTA CCAGATGCTG CAGGCCCTGA CGGAGGCCCC GGCGTCGGAG 
GGTTGCACCA TACCCCTGCT GTCCGGCCTG TCCAGAGCCG CCTATCCGCC GGAACTCCGA 
TTCATGTACT ATGTGGATGG CAGAGGCAAG GACGGAGGCT TTTACCAAGT CAAGGAGGCT 
GTTATACACT ATCTGGAGAC ACTAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heterotrimeric complex of p38 MAPK, PKC?, and TIRAP is required for AP1 mediated inflammatory response.
International immunopharmacology48211-218(2017 Jul)
Baig MS,Liu D,Muthu K,Roy A,Saqib U,Naim A,Faisal SM,Srivastava M,Saluja R

Human Adaptive Immunity Rescues an Inborn Error of Innate Immunity.
Cell168(5)789-800.e10(2017 Feb)
Israel L,Wang Y,Bulek K,Della Mina E,Zhang Z,Pedergnana V,Chrabieh M,Lemmens NA,Sancho-Shimizu V,Descatoire M,Lasseau T,Israelsson E,Lorenzo L,Yun L,Belkadi A,Moran A,Weisman LE,Vandenesch F,Batteux F,Weller S,Levin M,Herberg J,Abhyankar A,Prando C,Itan Y,van Wamel WJB,Picard C,Abel L,Chaussabel D,Li X,Beutler B,Arkwright PD,Casanova JL,Puel A

TIARP attenuates autoantibody-mediated arthritis via the suppression of neutrophil migration by reducing CXCL2/CXCR2 and IL-6 expression.
Scientific reports638684(2016 Dec)
Inoue A,Matsumoto I,Tanaka Y,Umeda N,Takai C,Kawaguchi H,Ebe H,Yoshida H,Matsumoto Y,Segawa S,Takahashi S,Sumida T

MyD88 Adapter-like (Mal)/TIRAP Is Required for Cytokine Production by Splenic Ly6CloTLR2hi but Not by Ly6ChiTLR2hi Monocytes during Trypanosoma cruzi Infection.
The Journal of biological chemistry291(45)23832-23841(2016 Nov)
Gravina HD,Goes AM,Murta SM,Ropert C

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Tirap cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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