Slc12a6 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc12a6
Entrez Gene ID	107723
Full Name	solute carrier family 12, member 6
Synonyms	9530023I19Rik,KCC3,gaxp
General protein information	Preferred Names solute carrier family 12, member 6 Names solute carrier family 12 member 6 K-Cl cotransporter 3 electroneutral potassium-chloride cotransporter 3
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_133648.2	NP_598409.2	solute carrier family 12 member 6 isoform 1
XM_006498546.3	XP_006498609.1	solute carrier family 12 member 6 isoform X2
NM_001362700.1	NP_001349629.1	solute carrier family 12 member 6 isoform 3
XM_006498545.3	XP_006498608.1	solute carrier family 12 member 6 isoform X1
NM_133649.2	NP_598410.2	solute carrier family 12 member 6 isoform 2
XM_006498546.4	XP_006498609.1	solute carrier family 12 member 6 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SLC12A6	NP_001180045.1
Mus musculus (house mouse)	Slc12a6	NP_598410.2
Xenopus tropicalis (tropical clawed frog)	slc12a6	NP_001072576.1
	SLC12A6	XP_002804750.1
Danio rerio (zebrafish)	slc12a7b	XP_696060.6
Homo sapiens (human)	SLC12A6	NP_598408.1
Pan troglodytes (chimpanzee)	SLC12A6	XP_001173480.1
Rattus norvegicus (Norway rat)	Slc12a6	NP_001103100.1
Canis lupus familiaris (dog)	SLC12A6	XP_005638240.1

Gene Ontology
Bibliography

Related articles in PubMed

KCC3 loss-of-function contributes to Andermann syndrome by inducing activity-dependent neuromuscular junction defects.
Bowerman M, Salsac C, Bernard V, Soulard C, Dionne A, Coque E, Benlefki S, Hince P, Dion PA, Butler-Browne G, Camu W, Bouchard JP, Delpire E, Rouleau GA, Raoul C, Scamps F
Neurobiology of disease10635-48(2017 Oct)

KCC3 deficiency-induced disruption of paranodal loops and impairment of axonal excitability in the peripheral nervous system.
Sun YT, Tzeng SF, Lin TS, Hsu KS, Delpire E, Shen MR
Neuroscience33591-102(2016 Oct)

Functional kinomics establishes a critical node of volume-sensitive cation-Cl
Zhang J, Gao G, Begum G, Wang J, Khanna AR, Shmukler BE, Daubner GM, de Los Heros P, Davies P, Varghese J, Bhuiyan MI, Duan J, Zhang J, Duran D, Alper SL, Sun D, Elledge SJ, Alessi DR, Kahle KT
Scientific reports635986(2016 Oct)

Peripheral motor neuropathy is associated with defective kinase regulation of the KCC3 cotransporter.
Kahle KT, Flores B, Bharucha-Goebel D, Zhang J, Donkervoort S, Hegde M, Hussain G, Duran D, Liang B, Sun D, B?nnemann CG, Delpire E
Science signaling9(439)ra77(2016 Aug)

Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria.
Garneau AP, Marcoux AA, No?l M, Frenette-Cotton R, Drolet MC, Couet J, Larivi?re R, Isenring P
PloS one11(5)e0154398(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

electrophysiological studies using the threshold tracking technique indicated a reduced stimulus-response curve slope with an elevated rheobase, a decreased strength-duration time constant, diminished persistent Na(+) currents, and an outward deviation of threshold electrotonus in KCC3(-/-) nerves compared to wild-type nerves
Title: KCC3 deficiency-induced disruption of paranodal loops and impairment of axonal excitability in the peripheral nervous system.

These results suggest that the function of the peripheral nervous system depends on finely tuned, kinase-regulated KCC3 activity and implicate abnormal cell volume homeostasis as a previously unreported mechanism of axonal degeneration.
Title: Peripheral motor neuropathy is associated with defective kinase regulation of the KCC3 cotransporter.

Kcc3 inactivation caused systemic vascular resistance and ventricular mass to increase while preventing extracellular fluid volume to accumulate.
Title: Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria.

The results establish that the parvalbumin-positive neuronal population is an important player in the pathogenic development of peripheral neuropathy associated with agenesis of the corpus callosum.
Title: Deletion of KCC3 in parvalbumin neurons leads to locomotor deficit in a conditional mouse model of peripheral neuropathy associated with agenesis of the corpus callosum.

Data are consistent with a role for KCC3 in the proximal tubule glucose reabsorption mechanism.
Title: Molecular evidence for a role for K(+)-Cl(-) cotransporters in the kidney.

The following Slc12a6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu29897	XM_006498546.4 Latest version!	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu15625	NM_133648.2 Latest version!	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu15775	NM_133649.2 Latest version!	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$678.30 $969.00
OMu29896	NM_001362700.1 Latest version!	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu29896	XM_006498545.3 Latest version!	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu29897	XM_006498546.3	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a6 ( XM_006498546.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498609.1 Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu29897
Clone ID Related Accession (Same CDS sequence)	XM_006498546.3 , XM_006498546.4
Accession Version	XM_006498546.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498546.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACT TCACTGTGAC CAAGGTAGAA GACCCAGAGG AGGGGGCAGC TGGCCCCCTC 
TCTCCTGAGC CCAGCTCAGC AGAAGTAAAA GCCCGGATTC AGGATCCCCA AGAACCAGAT 
GACGGCCATA AAAAAGCCCG AAATGCTTAT CTCAATAATT CCAACTATGA AGAAGGAGAC 
GAATATTTTG ATAAAAATTT GGCACTCTTT GAGGAAGAAA TGGACACCAG ACCAAAGGTG 
TCTTCTCTCC TCAACCGCAT GGCCAACTAT ACAAATCTGA CACAAGGAGC AAAGGAACAT 
GAAGAGGCAG AGAACATCAC TGAAGGGAAA AAGAAGCCTA CCAAGAGCCC CCAAATGGGT 
ACTTTCATGG GTGTCTACCT CCCATGTCTA CAGAACATCT TTGGAGTGAT CCTCTTCCTG 
CGTCTTACCT GGGTAGTGGG AACAGCTGGA ATCCTTCAGG CCTTTGCAAT TGTCCTCATC 
TGCTGCTGCT GTACAATGTT AACTGCCATC TCCATGAGCG CCATCGCCAC TAACGGAGTG 
GTGCCAGCTG GGGGCTCATA CTTCATGATT TCCAGAGCCC TGGGCCCAGA GTTTGGCGGG 
GCTGTAGGCC TCTGCTTTTA TCTTGGCACC ACATTTGCAG CAGCCATGTA TATTCTTGGT 
GCCATTGAAA TCTTTCTGGT ATACATTGTC CCCCGAGCTG CCATCTTTCG GAGTGACGAT 
GCACTCAAGG AGTCAGCAGC TATGCTGAAC AACATGCGCG TCTATGGTAC AGCCTTCTTG 
GTCCTCATGG TCTTGGTGGT ATTCATCGGC GTACGCTATG TGAATAAGTT TGCCTCACTC 
TTCCTGGCCT GTGTAATTGT GTCGATCTTG GCTATCTATG CTGGTGCCAT CAAGTCTTCC 
TTTGCTCCAC CACACTTCCC GGTCTGTATG CTGGGCAACC GTACCCTGTC ATCAAGACAC 
CTTGACATTT GCTCTAAGAC CAAGGAGGTT GACAACATGA CAGTACCATC AAAGTTATGG 
GGATTCTTCT GCAACTCGAG TCAGTTCTTT AATGCCACCT GTGATGAGTA CTTTGTTCAC 
AATAACGTCA TCTCAATCCA AGGCATTCCA GGGTTGGCTA GTGGTATCAT TACTGAAAAT 
CTTTGGAGTA ATTATTTACC AAAGGGTGAG ATAATTGAAA AGCCATCAGC CAAGTCATCT 
GATGTCTTAG GCAACTTAAA CCATGAATAT GTTCTTGCTG ATATCACCAC CTCCTTCACT 
CTGCTGGTGG GGATCTTCTT TCCCTCGGTC ACAGGTATCA TGGCTGGGTC AAACAGATCT 
GGAGATCTGA AAGATGCCCA GAAGTCTATT CCCATTGGGA CCATCCTTGC CATCCTGACC 
ACATCCTTTG TGTATTTAAG CAATGTTGTC CTTTTTGGTG CATGTATTGA AGGAGTCGTT 
CTCAGAGACA AATTTGGGGA TGCTGTAAAA GGGAATTTGG TTGTAGGCAC CTTATCCTGG 
CCATCCCCGT GGGTGATCGT TATTGGCTCC TTCTTTTCAA CATGTGGGGC TGGGCTGCAG 
AGCCTCACAG GTGCGCCTCG GCTGCTGCAG GCTATCGCCA AGGATAACAT CATACCTTTC 
CTTAGGGTTT TTGGTCACAG CAAAGCTAAT GGGGAACCTA CCTGGGCTTT ACTTCTAACT 
GCTGCCATAG CAGAGCTGGG AATTCTCATC GCCTCCCTGG ATCTCGTGGC CCCAATTCTT 
TCCATGTTTT TTCTCATGTG TTACCTCTTT GTGAACTTGG CTTGTGCCTT GCAAACATTG 
CTGCGAACCC CCAACTGGAG GCCTCGATTC CGCTATTATC ACTGGGCCCT CTCTTTCATG 
GGAATGAGTA TCTGTCTAGC TCTGATGTTC ATTTCTTCTT GGTATTATGC CATTGTAGCT 
ATGGTAATAG CTGGCATGAT CTACAAGTAC ATTGAATATC AAGGGGCTGA GAAAGAATGG 
GGGGATGGTA TCCGTGGGCT GTCGCTCAGT GCAGCCCGCT TCGCTTTGCT CCGTCTAGAG 
GAAGGACCTC CTCACACTAA AAACTGGAGG CCTCAGCTGC TCGTCCTACT GAAGCTGGAT 
GAAGATTTAC ACGTCAAGCA CCCTCGCCTC CTCACCTTTG CCTCCCAGCT CAAGGCAGGA 
AAGGGACTCA CGATTGTGGG CTCTGTCATC GTGGGGAACT TCTTGGAGAA CTATGGTGAC 
GCGCTCGCGG CAGAGCAGAC CATTAAGCAC CTAATGGAGG CAGAAAAGGT AAAAGGATTC 
TGCCAATTGG TGGTGGCTGC CAAGCTGAAA GAGGGCATTT CACACCTCAT CCAGTCCTGT 
GGCCTCGGAG GCATGAAACA CAACACAGTG GTGATGGGCT GGCCCAATGG CTGGCGTCAG 
AGTGAAGATG CTCGCGCTTG GAAGACTTTC ATTGGCACAG TACGAGTGAC AACTGCTGCC 
CATCTAGCCC TGCTGGTGGC TAAAAATGTC TCCTTCTTTC CCAGCAATGT GGAGCAGTTT 
TCTGAGGGCA ACATTGATGT GTGGTGGATT GTGCATGATG GGGGCATGCT CATGCTATTA 
CCGTTCCTGC TGAAACAGCA CAAGGTTTGG CGGAAATGCA GCATACGGAT CTTCACAGTA 
GCCCAACTAG AAGACAACAG TATCCAGATG AAGAAGGATC TGGCCACCTT TCTGTACCAC 
CTGCGCATTG AGGCAGAAGT GGAAGTGGTG GAGATGCACG ACAGTGACAT ATCTGCCTAT 
ACATATGAGC GCACCCTGAT GATGGAGCAG AGGTCCCAGA TGCTTCGGCA TATGCGGCTG 
TCCAAAACAG AGCGAGACAG GGAGGCACAG CTGGTGAAAG ATCGAAACTC AATGCTACGC 
TTGACCAGCA TTGGCTCTGA TGAGGACGAA GAGACAGAAA CGTACCAGGA GAAGGTGCAC 
ATGACTTGGA CCAAGGATAA ATACATGGCA TCCCGGGGGC AAAAGGTCAA GTCAATGGAA 
GGATTCCAGG ACCTACTTAA TATGCGTCCG GACCAGTCCA ACGTGAGACG GATGCATACA 
GCAGTGAAGC TCAATGAAGT TATAGTCAAC AAGTCTCATG AAGCAAAGCT GGTTTTGTTG 
AATATGCCAG GACCACCCCG GAACCCTGAA GGTGATGAAA ACTACATGGA ATTTCTAGAA 
GTGCTCACTG AGGGATTAGA ACGAGTCCTT CTTGTCCGGG GTGGTGGCAG TGAGGTCATC 
ACCATTTACT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498609.1
CDS	143..3397
Translation	MPHFTVTKVEDPEEGAAGPLSPEPSSAEVKARIQDPQEPDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	XM_006498546.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498546.4

ORF Insert Sequence:

ATGCCGCACT TCACTGTGAC CAAGGTAGAA GACCCAGAGG AGGGGGCAGC TGGCCCCCTC 
TCTCCTGAGC CCAGCTCAGC AGAAGTAAAA GCCCGGATTC AGGATCCCCA AGAACCAGAT 
GACGGCCATA AAAAAGCCCG AAATGCTTAT CTCAATAATT CCAACTATGA AGAAGGAGAC 
GAATATTTTG ATAAAAATTT GGCACTCTTT GAGGAAGAAA TGGACACCAG ACCAAAGGTG 
TCTTCTCTCC TCAACCGCAT GGCCAACTAT ACAAATCTGA CACAAGGAGC AAAGGAACAT 
GAAGAGGCAG AGAACATCAC TGAAGGGAAA AAGAAGCCTA CCAAGAGCCC CCAAATGGGT 
ACTTTCATGG GTGTCTACCT CCCATGTCTA CAGAACATCT TTGGAGTGAT CCTCTTCCTG 
CGTCTTACCT GGGTAGTGGG AACAGCTGGA ATCCTTCAGG CCTTTGCAAT TGTCCTCATC 
TGCTGCTGCT GTACAATGTT AACTGCCATC TCCATGAGCG CCATCGCCAC TAACGGAGTG 
GTGCCAGCTG GGGGCTCATA CTTCATGATT TCCAGAGCCC TGGGCCCAGA GTTTGGCGGG 
GCTGTAGGCC TCTGCTTTTA TCTTGGCACC ACATTTGCAG CAGCCATGTA TATTCTTGGT 
GCCATTGAAA TCTTTCTGGT ATACATTGTC CCCCGAGCTG CCATCTTTCG GAGTGACGAT 
GCACTCAAGG AGTCAGCAGC TATGCTGAAC AACATGCGCG TCTATGGTAC AGCCTTCTTG 
GTCCTCATGG TCTTGGTGGT ATTCATCGGC GTACGCTATG TGAATAAGTT TGCCTCACTC 
TTCCTGGCCT GTGTAATTGT GTCGATCTTG GCTATCTATG CTGGTGCCAT CAAGTCTTCC 
TTTGCTCCAC CACACTTCCC GGTCTGTATG CTGGGCAACC GTACCCTGTC ATCAAGACAC 
CTTGACATTT GCTCTAAGAC CAAGGAGGTT GACAACATGA CAGTACCATC AAAGTTATGG 
GGATTCTTCT GCAACTCGAG TCAGTTCTTT AATGCCACCT GTGATGAGTA CTTTGTTCAC 
AATAACGTCA TCTCAATCCA AGGCATTCCA GGGTTGGCTA GTGGTATCAT TACTGAAAAT 
CTTTGGAGTA ATTATTTACC AAAGGGTGAG ATAATTGAAA AGCCATCAGC CAAGTCATCT 
GATGTCTTAG GCAACTTAAA CCATGAATAT GTTCTTGCTG ATATCACCAC CTCCTTCACT 
CTGCTGGTGG GGATCTTCTT TCCCTCGGTC ACAGGTATCA TGGCTGGGTC AAACAGATCT 
GGAGATCTGA AAGATGCCCA GAAGTCTATT CCCATTGGGA CCATCCTTGC CATCCTGACC 
ACATCCTTTG TGTATTTAAG CAATGTTGTC CTTTTTGGTG CATGTATTGA AGGAGTCGTT 
CTCAGAGACA AATTTGGGGA TGCTGTAAAA GGGAATTTGG TTGTAGGCAC CTTATCCTGG 
CCATCCCCGT GGGTGATCGT TATTGGCTCC TTCTTTTCAA CATGTGGGGC TGGGCTGCAG 
AGCCTCACAG GTGCGCCTCG GCTGCTGCAG GCTATCGCCA AGGATAACAT CATACCTTTC 
CTTAGGGTTT TTGGTCACAG CAAAGCTAAT GGGGAACCTA CCTGGGCTTT ACTTCTAACT 
GCTGCCATAG CAGAGCTGGG AATTCTCATC GCCTCCCTGG ATCTCGTGGC CCCAATTCTT 
TCCATGTTTT TTCTCATGTG TTACCTCTTT GTGAACTTGG CTTGTGCCTT GCAAACATTG 
CTGCGAACCC CCAACTGGAG GCCTCGATTC CGCTATTATC ACTGGGCCCT CTCTTTCATG 
GGAATGAGTA TCTGTCTAGC TCTGATGTTC ATTTCTTCTT GGTATTATGC CATTGTAGCT 
ATGGTAATAG CTGGCATGAT CTACAAGTAC ATTGAATATC AAGGGGCTGA GAAAGAATGG 
GGGGATGGTA TCCGTGGGCT GTCGCTCAGT GCAGCCCGCT TCGCTTTGCT CCGTCTAGAG 
GAAGGACCTC CTCACACTAA AAACTGGAGG CCTCAGCTGC TCGTCCTACT GAAGCTGGAT 
GAAGATTTAC ACGTCAAGCA CCCTCGCCTC CTCACCTTTG CCTCCCAGCT CAAGGCAGGA 
AAGGGACTCA CGATTGTGGG CTCTGTCATC GTGGGGAACT TCTTGGAGAA CTATGGTGAC 
GCGCTCGCGG CAGAGCAGAC CATTAAGCAC CTAATGGAGG CAGAAAAGGT AAAAGGATTC 
TGCCAATTGG TGGTGGCTGC CAAGCTGAAA GAGGGCATTT CACACCTCAT CCAGTCCTGT 
GGCCTCGGAG GCATGAAACA CAACACAGTG GTGATGGGCT GGCCCAATGG CTGGCGTCAG 
AGTGAAGATG CTCGCGCTTG GAAGACTTTC ATTGGCACAG TACGAGTGAC AACTGCTGCC 
CATCTAGCCC TGCTGGTGGC TAAAAATGTC TCCTTCTTTC CCAGCAATGT GGAGCAGTTT 
TCTGAGGGCA ACATTGATGT GTGGTGGATT GTGCATGATG GGGGCATGCT CATGCTATTA 
CCGTTCCTGC TGAAACAGCA CAAGGTTTGG CGGAAATGCA GCATACGGAT CTTCACAGTA 
GCCCAACTAG AAGACAACAG TATCCAGATG AAGAAGGATC TGGCCACCTT TCTGTACCAC 
CTGCGCATTG AGGCAGAAGT GGAAGTGGTG GAGATGCACG ACAGTGACAT ATCTGCCTAT 
ACATATGAGC GCACCCTGAT GATGGAGCAG AGGTCCCAGA TGCTTCGGCA TATGCGGCTG 
TCCAAAACAG AGCGAGACAG GGAGGCACAG CTGGTGAAAG ATCGAAACTC AATGCTACGC 
TTGACCAGCA TTGGCTCTGA TGAGGACGAA GAGACAGAAA CGTACCAGGA GAAGGTGCAC 
ATGACTTGGA CCAAGGATAA ATACATGGCA TCCCGGGGGC AAAAGGTCAA GTCAATGGAA 
GGATTCCAGG ACCTACTTAA TATGCGTCCG GACCAGTCCA ACGTGAGACG GATGCATACA 
GCAGTGAAGC TCAATGAAGT TATAGTCAAC AAGTCTCATG AAGCAAAGCT GGTTTTGTTG 
AATATGCCAG GACCACCCCG GAACCCTGAA GGTGATGAAA ACTACATGGA ATTTCTAGAA 
GTGCTCACTG AGGGATTAGA ACGAGTCCTT CTTGTCCGGG GTGGTGGCAG TGAGGTCATC 
ACCATTTACT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a6 ( NM_133648.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598409.2 Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu15625
Clone ID Related Accession (Same CDS sequence)	NM_133648.2
Accession Version	NM_133648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 6 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL683897.5, AK133006.1, BC036323.1 and BC051061.1. On Sep 5, 2007 this sequence version replaced NM_133648.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF211855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGCACT TCACTGTGAC CAAGGTAGAA GACCCAGAGG AGGGGGCAGC TGGCCCCCTC 
TCTCCTGAGC CCAGCTCAGC AGAAGTAAAA GCCCGGATTC AGGATCCCCA AGAACCAGAC 
CCGAGTCAGA ACTCCATCAC AGGGGAGCAC AGCCAGCTGT TAGATGACGG CCATAAAAAA 
GCCCGAAATG CTTATCTCAA TAATTCCAAC TATGAAGAAG GAGACGAATA TTTTGATAAA 
AATTTGGCAC TCTTTGAGGA AGAAATGGAC ACCAGACCAA AGGTGTCTTC TCTCCTCAAC 
CGCATGGCCA ACTATACAAA TCTGACACAA GGAGCAAAGG AACATGAAGA GGCAGAGAAC 
ATCACTGAAG GGAAAAAGAA GCCTACCAAG AGCCCCCAAA TGGGTACTTT CATGGGTGTC 
TACCTCCCAT GTCTACAGAA CATCTTTGGA GTGATCCTCT TCCTGCGTCT TACCTGGGTA 
GTGGGAACAG CTGGAATCCT TCAGGCCTTT GCAATTGTCC TCATCTGCTG CTGCTGTACA 
ATGTTAACTG CCATCTCCAT GAGCGCCATC GCCACTAACG GAGTGGTGCC AGCTGGGGGC 
TCATACTTCA TGATTTCCAG AGCCCTGGGC CCAGAGTTTG GCGGGGCTGT AGGCCTCTGC 
TTTTATCTTG GCACCACATT TGCAGCAGCC ATGTATATTC TTGGTGCCAT TGAAATCTTT 
CTGGTATACA TTGTCCCCCG AGCTGCCATC TTTCGGAGTG ACGATGCACT CAAGGAGTCA 
GCAGCTATGC TGAACAACAT GCGCGTCTAT GGTACAGCCT TCTTGGTCCT CATGGTCTTG 
GTGGTATTCA TCGGCGTACG CTATGTGAAT AAGTTTGCCT CACTCTTCCT GGCCTGTGTA 
ATTGTGTCGA TCTTGGCTAT CTATGCTGGT GCCATCAAGT CTTCCTTTGC TCCACCACAC 
TTCCCGGTCT GTATGCTGGG CAACCGTACC CTGTCATCAA GACACCTTGA CATTTGCTCT 
AAGACCAAGG AGGTTGACAA CATGACAGTA CCATCAAAGT TATGGGGATT CTTCTGCAAC 
TCGAGTCAGT TCTTTAATGC CACCTGTGAT GAGTACTTTG TTCACAATAA CGTCATCTCA 
ATCCAAGGCA TTCCAGGGTT GGCTAGTGGT ATCATTACTG AAAATCTTTG GAGTAATTAT 
TTACCAAAGG GTGAGATAAT TGAAAAGCCA TCAGCCAAGT CATCTGATGT CTTAGGCAAC 
TTAAACCATG AATATGTTCT TGCTGATATC ACCACCTCCT TCACTCTGCT GGTGGGGATC 
TTCTTTCCCT CGGTCACAGG TATCATGGCT GGGTCAAACA GATCTGGAGA TCTGAAAGAT 
GCCCAGAAGT CTATTCCCAT TGGGACCATC CTTGCCATCC TGACCACATC CTTTGTGTAT 
TTAAGCAATG TTGTCCTTTT TGGTGCATGT ATTGAAGGAG TCGTTCTCAG AGACAAATTT 
GGGGATGCTG TAAAAGGGAA TTTGGTTGTA GGCACCTTAT CCTGGCCATC CCCGTGGGTG 
ATCGTTATTG GCTCCTTCTT TTCAACATGT GGGGCTGGGC TGCAGAGCCT CACAGGTGCG 
CCTCGGCTGC TGCAGGCTAT CGCCAAGGAT AACATCATAC CTTTCCTTAG GGTTTTTGGT 
CACAGCAAAG CTAATGGGGA ACCTACCTGG GCTTTACTTC TAACTGCTGC CATAGCAGAG 
CTGGGAATTC TCATCGCCTC CCTGGATCTC GTGGCCCCAA TTCTTTCCAT GTTTTTTCTC 
ATGTGTTACC TCTTTGTGAA CTTGGCTTGT GCCTTGCAAA CATTGCTGCG AACCCCCAAC 
TGGAGGCCTC GATTCCGCTA TTATCACTGG GCCCTCTCTT TCATGGGAAT GAGTATCTGT 
CTAGCTCTGA TGTTCATTTC TTCTTGGTAT TATGCCATTG TAGCTATGGT AATAGCTGGC 
ATGATCTACA AGTACATTGA ATATCAAGGG GCTGAGAAAG AATGGGGGGA TGGTATCCGT 
GGGCTGTCGC TCAGTGCAGC CCGCTTCGCT TTGCTCCGTC TAGAGGAAGG ACCTCCTCAC 
ACTAAAAACT GGAGGCCTCA GCTGCTCGTC CTACTGAAGC TGGATGAAGA TTTACACGTC 
AAGCACCCTC GCCTCCTCAC CTTTGCCTCC CAGCTCAAGG CAGGAAAGGG ACTCACGATT 
GTGGGCTCTG TCATCGTGGG GAACTTCTTG GAGAACTATG GTGACGCGCT CGCGGCAGAG 
CAGACCATTA AGCACCTAAT GGAGGCAGAA AAGGTAAAAG GATTCTGCCA ATTGGTGGTG 
GCTGCCAAGC TGAAAGAGGG CATTTCACAC CTCATCCAGT CCTGTGGCCT CGGAGGCATG 
AAACACAACA CAGTGGTGAT GGGCTGGCCC AATGGCTGGC GTCAGAGTGA AGATGCTCGC 
GCTTGGAAGA CTTTCATTGG CACAGTACGA GTGACAACTG CTGCCCATCT AGCCCTGCTG 
GTGGCTAAAA ATGTCTCCTT CTTTCCCAGC AATGTGGAGC AGTTTTCTGA GGGCAACATT 
GATGTGTGGT GGATTGTGCA TGATGGGGGC ATGCTCATGC TATTACCGTT CCTGCTGAAA 
CAGCACAAGG TTTGGCGGAA ATGCAGCATA CGGATCTTCA CAGTAGCCCA ACTAGAAGAC 
AACAGTATCC AGATGAAGAA GGATCTGGCC ACCTTTCTGT ACCACCTGCG CATTGAGGCA 
GAAGTGGAAG TGGTGGAGAT GCACGACAGT GACATATCTG CCTATACATA TGAGCGCACC 
CTGATGATGG AGCAGAGGTC CCAGATGCTT CGGCATATGC GGCTGTCCAA AACAGAGCGA 
GACAGGGAGG CACAGCTGGT GAAAGATCGA AACTCAATGC TACGCTTGAC CAGCATTGGC 
TCTGATGAGG ACGAAGAGAC AGAAACGTAC CAGGAGAAGG TGCACATGAC TTGGACCAAG 
GATAAATACA TGGCATCCCG GGGGCAAAAG GTCAAGTCAA TGGAAGGATT CCAGGACCTA 
CTTAATATGC GTCCGGACCA GTCCAACGTG AGACGGATGC ATACAGCAGT GAAGCTCAAT 
GAAGTTATAG TCAACAAGTC TCATGAAGCA AAGCTGGTTT TGTTGAATAT GCCAGGACCA 
CCCCGGAACC CTGAAGGTGA TGAAAACTAC ATGGAATTTC TAGAAGTGCT CACTGAGGGA 
TTAGAACGAG TCCTTCTTGT CCGGGGTGGT GGCAGTGAGG TCATCACCAT TTACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598409.2
CDS	1..3300
Translation	MPHFTVTKVEDPEEGAAGPLSPEPSSAEVKARIQDPQEPDPSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	NM_133648.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133648.2

ORF Insert Sequence:

ATGCCGCACT TCACTGTGAC CAAGGTAGAA GACCCAGAGG AGGGGGCAGC TGGCCCCCTC 
TCTCCTGAGC CCAGCTCAGC AGAAGTAAAA GCCCGGATTC AGGATCCCCA AGAACCAGAC 
CCGAGTCAGA ACTCCATCAC AGGGGAGCAC AGCCAGCTGT TAGATGACGG CCATAAAAAA 
GCCCGAAATG CTTATCTCAA TAATTCCAAC TATGAAGAAG GAGACGAATA TTTTGATAAA 
AATTTGGCAC TCTTTGAGGA AGAAATGGAC ACCAGACCAA AGGTGTCTTC TCTCCTCAAC 
CGCATGGCCA ACTATACAAA TCTGACACAA GGAGCAAAGG AACATGAAGA GGCAGAGAAC 
ATCACTGAAG GGAAAAAGAA GCCTACCAAG AGCCCCCAAA TGGGTACTTT CATGGGTGTC 
TACCTCCCAT GTCTACAGAA CATCTTTGGA GTGATCCTCT TCCTGCGTCT TACCTGGGTA 
GTGGGAACAG CTGGAATCCT TCAGGCCTTT GCAATTGTCC TCATCTGCTG CTGCTGTACA 
ATGTTAACTG CCATCTCCAT GAGCGCCATC GCCACTAACG GAGTGGTGCC AGCTGGGGGC 
TCATACTTCA TGATTTCCAG AGCCCTGGGC CCAGAGTTTG GCGGGGCTGT AGGCCTCTGC 
TTTTATCTTG GCACCACATT TGCAGCAGCC ATGTATATTC TTGGTGCCAT TGAAATCTTT 
CTGGTATACA TTGTCCCCCG AGCTGCCATC TTTCGGAGTG ACGATGCACT CAAGGAGTCA 
GCAGCTATGC TGAACAACAT GCGCGTCTAT GGTACAGCCT TCTTGGTCCT CATGGTCTTG 
GTGGTATTCA TCGGCGTACG CTATGTGAAT AAGTTTGCCT CACTCTTCCT GGCCTGTGTA 
ATTGTGTCGA TCTTGGCTAT CTATGCTGGT GCCATCAAGT CTTCCTTTGC TCCACCACAC 
TTCCCGGTCT GTATGCTGGG CAACCGTACC CTGTCATCAA GACACCTTGA CATTTGCTCT 
AAGACCAAGG AGGTTGACAA CATGACAGTA CCATCAAAGT TATGGGGATT CTTCTGCAAC 
TCGAGTCAGT TCTTTAATGC CACCTGTGAT GAGTACTTTG TTCACAATAA CGTCATCTCA 
ATCCAAGGCA TTCCAGGGTT GGCTAGTGGT ATCATTACTG AAAATCTTTG GAGTAATTAT 
TTACCAAAGG GTGAGATAAT TGAAAAGCCA TCAGCCAAGT CATCTGATGT CTTAGGCAAC 
TTAAACCATG AATATGTTCT TGCTGATATC ACCACCTCCT TCACTCTGCT GGTGGGGATC 
TTCTTTCCCT CGGTCACAGG TATCATGGCT GGGTCAAACA GATCTGGAGA TCTGAAAGAT 
GCCCAGAAGT CTATTCCCAT TGGGACCATC CTTGCCATCC TGACCACATC CTTTGTGTAT 
TTAAGCAATG TTGTCCTTTT TGGTGCATGT ATTGAAGGAG TCGTTCTCAG AGACAAATTT 
GGGGATGCTG TAAAAGGGAA TTTGGTTGTA GGCACCTTAT CCTGGCCATC CCCGTGGGTG 
ATCGTTATTG GCTCCTTCTT TTCAACATGT GGGGCTGGGC TGCAGAGCCT CACAGGTGCG 
CCTCGGCTGC TGCAGGCTAT CGCCAAGGAT AACATCATAC CTTTCCTTAG GGTTTTTGGT 
CACAGCAAAG CTAATGGGGA ACCTACCTGG GCTTTACTTC TAACTGCTGC CATAGCAGAG 
CTGGGAATTC TCATCGCCTC CCTGGATCTC GTGGCCCCAA TTCTTTCCAT GTTTTTTCTC 
ATGTGTTACC TCTTTGTGAA CTTGGCTTGT GCCTTGCAAA CATTGCTGCG AACCCCCAAC 
TGGAGGCCTC GATTCCGCTA TTATCACTGG GCCCTCTCTT TCATGGGAAT GAGTATCTGT 
CTAGCTCTGA TGTTCATTTC TTCTTGGTAT TATGCCATTG TAGCTATGGT AATAGCTGGC 
ATGATCTACA AGTACATTGA ATATCAAGGG GCTGAGAAAG AATGGGGGGA TGGTATCCGT 
GGGCTGTCGC TCAGTGCAGC CCGCTTCGCT TTGCTCCGTC TAGAGGAAGG ACCTCCTCAC 
ACTAAAAACT GGAGGCCTCA GCTGCTCGTC CTACTGAAGC TGGATGAAGA TTTACACGTC 
AAGCACCCTC GCCTCCTCAC CTTTGCCTCC CAGCTCAAGG CAGGAAAGGG ACTCACGATT 
GTGGGCTCTG TCATCGTGGG GAACTTCTTG GAGAACTATG GTGACGCGCT CGCGGCAGAG 
CAGACCATTA AGCACCTAAT GGAGGCAGAA AAGGTAAAAG GATTCTGCCA ATTGGTGGTG 
GCTGCCAAGC TGAAAGAGGG CATTTCACAC CTCATCCAGT CCTGTGGCCT CGGAGGCATG 
AAACACAACA CAGTGGTGAT GGGCTGGCCC AATGGCTGGC GTCAGAGTGA AGATGCTCGC 
GCTTGGAAGA CTTTCATTGG CACAGTACGA GTGACAACTG CTGCCCATCT AGCCCTGCTG 
GTGGCTAAAA ATGTCTCCTT CTTTCCCAGC AATGTGGAGC AGTTTTCTGA GGGCAACATT 
GATGTGTGGT GGATTGTGCA TGATGGGGGC ATGCTCATGC TATTACCGTT CCTGCTGAAA 
CAGCACAAGG TTTGGCGGAA ATGCAGCATA CGGATCTTCA CAGTAGCCCA ACTAGAAGAC 
AACAGTATCC AGATGAAGAA GGATCTGGCC ACCTTTCTGT ACCACCTGCG CATTGAGGCA 
GAAGTGGAAG TGGTGGAGAT GCACGACAGT GACATATCTG CCTATACATA TGAGCGCACC 
CTGATGATGG AGCAGAGGTC CCAGATGCTT CGGCATATGC GGCTGTCCAA AACAGAGCGA 
GACAGGGAGG CACAGCTGGT GAAAGATCGA AACTCAATGC TACGCTTGAC CAGCATTGGC 
TCTGATGAGG ACGAAGAGAC AGAAACGTAC CAGGAGAAGG TGCACATGAC TTGGACCAAG 
GATAAATACA TGGCATCCCG GGGGCAAAAG GTCAAGTCAA TGGAAGGATT CCAGGACCTA 
CTTAATATGC GTCCGGACCA GTCCAACGTG AGACGGATGC ATACAGCAGT GAAGCTCAAT 
GAAGTTATAG TCAACAAGTC TCATGAAGCA AAGCTGGTTT TGTTGAATAT GCCAGGACCA 
CCCCGGAACC CTGAAGGTGA TGAAAACTAC ATGGAATTTC TAGAAGTGCT CACTGAGGGA 
TTAGAACGAG TCCTTCTTGT CCGGGGTGGT GGCAGTGAGG TCATCACCAT TTACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a6 ( NM_133649.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598410.2 Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 2, mRNA.

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CloneID	OMu15775
Clone ID Related Accession (Same CDS sequence)	NM_133649.2
Accession Version	NM_133649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 6 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133006.1, BC036323.1 and BC051061.1. On Sep 5, 2007 this sequence version replaced NM_133649.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF211854.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATCCAC CAGAAGCCAC CACCAAGATG TCCTCAGTTC GGTTCATGGT GACACCAACT 
AAGATTGATG ACATTCCAGG TTTGTCAGAC ACCAGCCCGG ACCTCAGCTC TCGGTCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGCGAACTGT CTGGGGCTAC TACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ATCCCCAGGA TGTTACGGAG GACCCGAGTC AGAACTCCAT CACAGGGGAG 
CACAGCCAGC TGTTAGATGA CGGCCATAAA AAAGCCCGAA ATGCTTATCT CAATAATTCC 
AACTATGAAG AAGGAGACGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG 
GACACCAGAC CAAAGGTGTC TTCTCTCCTC AACCGCATGG CCAACTATAC AAATCTGACA 
CAAGGAGCAA AGGAACATGA AGAGGCAGAG AACATCACTG AAGGGAAAAA GAAGCCTACC 
AAGAGCCCCC AAATGGGTAC TTTCATGGGT GTCTACCTCC CATGTCTACA GAACATCTTT 
GGAGTGATCC TCTTCCTGCG TCTTACCTGG GTAGTGGGAA CAGCTGGAAT CCTTCAGGCC 
TTTGCAATTG TCCTCATCTG CTGCTGCTGT ACAATGTTAA CTGCCATCTC CATGAGCGCC 
ATCGCCACTA ACGGAGTGGT GCCAGCTGGG GGCTCATACT TCATGATTTC CAGAGCCCTG 
GGCCCAGAGT TTGGCGGGGC TGTAGGCCTC TGCTTTTATC TTGGCACCAC ATTTGCAGCA 
GCCATGTATA TTCTTGGTGC CATTGAAATC TTTCTGGTAT ACATTGTCCC CCGAGCTGCC 
ATCTTTCGGA GTGACGATGC ACTCAAGGAG TCAGCAGCTA TGCTGAACAA CATGCGCGTC 
TATGGTACAG CCTTCTTGGT CCTCATGGTC TTGGTGGTAT TCATCGGCGT ACGCTATGTG 
AATAAGTTTG CCTCACTCTT CCTGGCCTGT GTAATTGTGT CGATCTTGGC TATCTATGCT 
GGTGCCATCA AGTCTTCCTT TGCTCCACCA CACTTCCCGG TCTGTATGCT GGGCAACCGT 
ACCCTGTCAT CAAGACACCT TGACATTTGC TCTAAGACCA AGGAGGTTGA CAACATGACA 
GTACCATCAA AGTTATGGGG ATTCTTCTGC AACTCGAGTC AGTTCTTTAA TGCCACCTGT 
GATGAGTACT TTGTTCACAA TAACGTCATC TCAATCCAAG GCATTCCAGG GTTGGCTAGT 
GGTATCATTA CTGAAAATCT TTGGAGTAAT TATTTACCAA AGGGTGAGAT AATTGAAAAG 
CCATCAGCCA AGTCATCTGA TGTCTTAGGC AACTTAAACC ATGAATATGT TCTTGCTGAT 
ATCACCACCT CCTTCACTCT GCTGGTGGGG ATCTTCTTTC CCTCGGTCAC AGGTATCATG 
GCTGGGTCAA ACAGATCTGG AGATCTGAAA GATGCCCAGA AGTCTATTCC CATTGGGACC 
ATCCTTGCCA TCCTGACCAC ATCCTTTGTG TATTTAAGCA ATGTTGTCCT TTTTGGTGCA 
TGTATTGAAG GAGTCGTTCT CAGAGACAAA TTTGGGGATG CTGTAAAAGG GAATTTGGTT 
GTAGGCACCT TATCCTGGCC ATCCCCGTGG GTGATCGTTA TTGGCTCCTT CTTTTCAACA 
TGTGGGGCTG GGCTGCAGAG CCTCACAGGT GCGCCTCGGC TGCTGCAGGC TATCGCCAAG 
GATAACATCA TACCTTTCCT TAGGGTTTTT GGTCACAGCA AAGCTAATGG GGAACCTACC 
TGGGCTTTAC TTCTAACTGC TGCCATAGCA GAGCTGGGAA TTCTCATCGC CTCCCTGGAT 
CTCGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT GAACTTGGCT 
TGTGCCTTGC AAACATTGCT GCGAACCCCC AACTGGAGGC CTCGATTCCG CTATTATCAC 
TGGGCCCTCT CTTTCATGGG AATGAGTATC TGTCTAGCTC TGATGTTCAT TTCTTCTTGG 
TATTATGCCA TTGTAGCTAT GGTAATAGCT GGCATGATCT ACAAGTACAT TGAATATCAA 
GGGGCTGAGA AAGAATGGGG GGATGGTATC CGTGGGCTGT CGCTCAGTGC AGCCCGCTTC 
GCTTTGCTCC GTCTAGAGGA AGGACCTCCT CACACTAAAA ACTGGAGGCC TCAGCTGCTC 
GTCCTACTGA AGCTGGATGA AGATTTACAC GTCAAGCACC CTCGCCTCCT CACCTTTGCC 
TCCCAGCTCA AGGCAGGAAA GGGACTCACG ATTGTGGGCT CTGTCATCGT GGGGAACTTC 
TTGGAGAACT ATGGTGACGC GCTCGCGGCA GAGCAGACCA TTAAGCACCT AATGGAGGCA 
GAAAAGGTAA AAGGATTCTG CCAATTGGTG GTGGCTGCCA AGCTGAAAGA GGGCATTTCA 
CACCTCATCC AGTCCTGTGG CCTCGGAGGC ATGAAACACA ACACAGTGGT GATGGGCTGG 
CCCAATGGCT GGCGTCAGAG TGAAGATGCT CGCGCTTGGA AGACTTTCAT TGGCACAGTA 
CGAGTGACAA CTGCTGCCCA TCTAGCCCTG CTGGTGGCTA AAAATGTCTC CTTCTTTCCC 
AGCAATGTGG AGCAGTTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG 
GGCATGCTCA TGCTATTACC GTTCCTGCTG AAACAGCACA AGGTTTGGCG GAAATGCAGC 
ATACGGATCT TCACAGTAGC CCAACTAGAA GACAACAGTA TCCAGATGAA GAAGGATCTG 
GCCACCTTTC TGTACCACCT GCGCATTGAG GCAGAAGTGG AAGTGGTGGA GATGCACGAC 
AGTGACATAT CTGCCTATAC ATATGAGCGC ACCCTGATGA TGGAGCAGAG GTCCCAGATG 
CTTCGGCATA TGCGGCTGTC CAAAACAGAG CGAGACAGGG AGGCACAGCT GGTGAAAGAT 
CGAAACTCAA TGCTACGCTT GACCAGCATT GGCTCTGATG AGGACGAAGA GACAGAAACG 
TACCAGGAGA AGGTGCACAT GACTTGGACC AAGGATAAAT ACATGGCATC CCGGGGGCAA 
AAGGTCAAGT CAATGGAAGG ATTCCAGGAC CTACTTAATA TGCGTCCGGA CCAGTCCAAC 
GTGAGACGGA TGCATACAGC AGTGAAGCTC AATGAAGTTA TAGTCAACAA GTCTCATGAA 
GCAAAGCTGG TTTTGTTGAA TATGCCAGGA CCACCCCGGA ACCCTGAAGG TGATGAAAAC 
TACATGGAAT TTCTAGAAGT GCTCACTGAG GGATTAGAAC GAGTCCTTCT TGTCCGGGGT 
GGTGGCAGTG AGGTCATCAC CATTTACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598410.2
CDS	509..3961
Translation	MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSLATVALDPSSDRTSNPQDVTEDPSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	NM_133649.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133649.2

ORF Insert Sequence:

ATGCATCCAC CAGAAGCCAC CACCAAGATG TCCTCAGTTC GGTTCATGGT GACACCAACT 
AAGATTGATG ACATTCCAGG TTTGTCAGAC ACCAGCCCGG ACCTCAGCTC TCGGTCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGCGAACTGT CTGGGGCTAC TACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ATCCCCAGGA TGTTACGGAG GACCCGAGTC AGAACTCCAT CACAGGGGAG 
CACAGCCAGC TGTTAGATGA CGGCCATAAA AAAGCCCGAA ATGCTTATCT CAATAATTCC 
AACTATGAAG AAGGAGACGA ATATTTTGAT AAAAATTTGG CACTCTTTGA GGAAGAAATG 
GACACCAGAC CAAAGGTGTC TTCTCTCCTC AACCGCATGG CCAACTATAC AAATCTGACA 
CAAGGAGCAA AGGAACATGA AGAGGCAGAG AACATCACTG AAGGGAAAAA GAAGCCTACC 
AAGAGCCCCC AAATGGGTAC TTTCATGGGT GTCTACCTCC CATGTCTACA GAACATCTTT 
GGAGTGATCC TCTTCCTGCG TCTTACCTGG GTAGTGGGAA CAGCTGGAAT CCTTCAGGCC 
TTTGCAATTG TCCTCATCTG CTGCTGCTGT ACAATGTTAA CTGCCATCTC CATGAGCGCC 
ATCGCCACTA ACGGAGTGGT GCCAGCTGGG GGCTCATACT TCATGATTTC CAGAGCCCTG 
GGCCCAGAGT TTGGCGGGGC TGTAGGCCTC TGCTTTTATC TTGGCACCAC ATTTGCAGCA 
GCCATGTATA TTCTTGGTGC CATTGAAATC TTTCTGGTAT ACATTGTCCC CCGAGCTGCC 
ATCTTTCGGA GTGACGATGC ACTCAAGGAG TCAGCAGCTA TGCTGAACAA CATGCGCGTC 
TATGGTACAG CCTTCTTGGT CCTCATGGTC TTGGTGGTAT TCATCGGCGT ACGCTATGTG 
AATAAGTTTG CCTCACTCTT CCTGGCCTGT GTAATTGTGT CGATCTTGGC TATCTATGCT 
GGTGCCATCA AGTCTTCCTT TGCTCCACCA CACTTCCCGG TCTGTATGCT GGGCAACCGT 
ACCCTGTCAT CAAGACACCT TGACATTTGC TCTAAGACCA AGGAGGTTGA CAACATGACA 
GTACCATCAA AGTTATGGGG ATTCTTCTGC AACTCGAGTC AGTTCTTTAA TGCCACCTGT 
GATGAGTACT TTGTTCACAA TAACGTCATC TCAATCCAAG GCATTCCAGG GTTGGCTAGT 
GGTATCATTA CTGAAAATCT TTGGAGTAAT TATTTACCAA AGGGTGAGAT AATTGAAAAG 
CCATCAGCCA AGTCATCTGA TGTCTTAGGC AACTTAAACC ATGAATATGT TCTTGCTGAT 
ATCACCACCT CCTTCACTCT GCTGGTGGGG ATCTTCTTTC CCTCGGTCAC AGGTATCATG 
GCTGGGTCAA ACAGATCTGG AGATCTGAAA GATGCCCAGA AGTCTATTCC CATTGGGACC 
ATCCTTGCCA TCCTGACCAC ATCCTTTGTG TATTTAAGCA ATGTTGTCCT TTTTGGTGCA 
TGTATTGAAG GAGTCGTTCT CAGAGACAAA TTTGGGGATG CTGTAAAAGG GAATTTGGTT 
GTAGGCACCT TATCCTGGCC ATCCCCGTGG GTGATCGTTA TTGGCTCCTT CTTTTCAACA 
TGTGGGGCTG GGCTGCAGAG CCTCACAGGT GCGCCTCGGC TGCTGCAGGC TATCGCCAAG 
GATAACATCA TACCTTTCCT TAGGGTTTTT GGTCACAGCA AAGCTAATGG GGAACCTACC 
TGGGCTTTAC TTCTAACTGC TGCCATAGCA GAGCTGGGAA TTCTCATCGC CTCCCTGGAT 
CTCGTGGCCC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT GAACTTGGCT 
TGTGCCTTGC AAACATTGCT GCGAACCCCC AACTGGAGGC CTCGATTCCG CTATTATCAC 
TGGGCCCTCT CTTTCATGGG AATGAGTATC TGTCTAGCTC TGATGTTCAT TTCTTCTTGG 
TATTATGCCA TTGTAGCTAT GGTAATAGCT GGCATGATCT ACAAGTACAT TGAATATCAA 
GGGGCTGAGA AAGAATGGGG GGATGGTATC CGTGGGCTGT CGCTCAGTGC AGCCCGCTTC 
GCTTTGCTCC GTCTAGAGGA AGGACCTCCT CACACTAAAA ACTGGAGGCC TCAGCTGCTC 
GTCCTACTGA AGCTGGATGA AGATTTACAC GTCAAGCACC CTCGCCTCCT CACCTTTGCC 
TCCCAGCTCA AGGCAGGAAA GGGACTCACG ATTGTGGGCT CTGTCATCGT GGGGAACTTC 
TTGGAGAACT ATGGTGACGC GCTCGCGGCA GAGCAGACCA TTAAGCACCT AATGGAGGCA 
GAAAAGGTAA AAGGATTCTG CCAATTGGTG GTGGCTGCCA AGCTGAAAGA GGGCATTTCA 
CACCTCATCC AGTCCTGTGG CCTCGGAGGC ATGAAACACA ACACAGTGGT GATGGGCTGG 
CCCAATGGCT GGCGTCAGAG TGAAGATGCT CGCGCTTGGA AGACTTTCAT TGGCACAGTA 
CGAGTGACAA CTGCTGCCCA TCTAGCCCTG CTGGTGGCTA AAAATGTCTC CTTCTTTCCC 
AGCAATGTGG AGCAGTTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT GCATGATGGG 
GGCATGCTCA TGCTATTACC GTTCCTGCTG AAACAGCACA AGGTTTGGCG GAAATGCAGC 
ATACGGATCT TCACAGTAGC CCAACTAGAA GACAACAGTA TCCAGATGAA GAAGGATCTG 
GCCACCTTTC TGTACCACCT GCGCATTGAG GCAGAAGTGG AAGTGGTGGA GATGCACGAC 
AGTGACATAT CTGCCTATAC ATATGAGCGC ACCCTGATGA TGGAGCAGAG GTCCCAGATG 
CTTCGGCATA TGCGGCTGTC CAAAACAGAG CGAGACAGGG AGGCACAGCT GGTGAAAGAT 
CGAAACTCAA TGCTACGCTT GACCAGCATT GGCTCTGATG AGGACGAAGA GACAGAAACG 
TACCAGGAGA AGGTGCACAT GACTTGGACC AAGGATAAAT ACATGGCATC CCGGGGGCAA 
AAGGTCAAGT CAATGGAAGG ATTCCAGGAC CTACTTAATA TGCGTCCGGA CCAGTCCAAC 
GTGAGACGGA TGCATACAGC AGTGAAGCTC AATGAAGTTA TAGTCAACAA GTCTCATGAA 
GCAAAGCTGG TTTTGTTGAA TATGCCAGGA CCACCCCGGA ACCCTGAAGG TGATGAAAAC 
TACATGGAAT TTCTAGAAGT GCTCACTGAG GGATTAGAAC GAGTCCTTCT TGTCCGGGGT 
GGTGGCAGTG AGGTCATCAC CATTTACTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a6 ( NM_001362700.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001349629.1 Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 3, mRNA.

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CloneID	OMu29896
Clone ID Related Accession (Same CDS sequence)	NM_001362700.1 , XM_006498545.3
Accession Version	NM_001362700.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-10
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 6 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL713853.7 and AL683897.5. On Apr 25, 2018 this sequence version replaced XM_006498545.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.166273.1, SRR1660825.64954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCATCCAC CAGAAGCCAC CACCAAGATG TCCTCAGTTC GGTTCATGGT GACACCAACT 
AAGATTGATG ACATTCCAGG TTTGTCAGAC ACCAGCCCGG ACCTCAGCTC TCGGTCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGCGAACTGT CTGGGGCTAC TACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ATCCCCAGGA TGTTACGGAG GATGACGGCC ATAAAAAAGC CCGAAATGCT 
TATCTCAATA ATTCCAACTA TGAAGAAGGA GACGAATATT TTGATAAAAA TTTGGCACTC 
TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTCAACCG CATGGCCAAC 
TATACAAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG 
AAAAAGAAGC CTACCAAGAG CCCCCAAATG GGTACTTTCA TGGGTGTCTA CCTCCCATGT 
CTACAGAACA TCTTTGGAGT GATCCTCTTC CTGCGTCTTA CCTGGGTAGT GGGAACAGCT 
GGAATCCTTC AGGCCTTTGC AATTGTCCTC ATCTGCTGCT GCTGTACAAT GTTAACTGCC 
ATCTCCATGA GCGCCATCGC CACTAACGGA GTGGTGCCAG CTGGGGGCTC ATACTTCATG 
ATTTCCAGAG CCCTGGGCCC AGAGTTTGGC GGGGCTGTAG GCCTCTGCTT TTATCTTGGC 
ACCACATTTG CAGCAGCCAT GTATATTCTT GGTGCCATTG AAATCTTTCT GGTATACATT 
GTCCCCCGAG CTGCCATCTT TCGGAGTGAC GATGCACTCA AGGAGTCAGC AGCTATGCTG 
AACAACATGC GCGTCTATGG TACAGCCTTC TTGGTCCTCA TGGTCTTGGT GGTATTCATC 
GGCGTACGCT ATGTGAATAA GTTTGCCTCA CTCTTCCTGG CCTGTGTAAT TGTGTCGATC 
TTGGCTATCT ATGCTGGTGC CATCAAGTCT TCCTTTGCTC CACCACACTT CCCGGTCTGT 
ATGCTGGGCA ACCGTACCCT GTCATCAAGA CACCTTGACA TTTGCTCTAA GACCAAGGAG 
GTTGACAACA TGACAGTACC ATCAAAGTTA TGGGGATTCT TCTGCAACTC GAGTCAGTTC 
TTTAATGCCA CCTGTGATGA GTACTTTGTT CACAATAACG TCATCTCAAT CCAAGGCATT 
CCAGGGTTGG CTAGTGGTAT CATTACTGAA AATCTTTGGA GTAATTATTT ACCAAAGGGT 
GAGATAATTG AAAAGCCATC AGCCAAGTCA TCTGATGTCT TAGGCAACTT AAACCATGAA 
TATGTTCTTG CTGATATCAC CACCTCCTTC ACTCTGCTGG TGGGGATCTT CTTTCCCTCG 
GTCACAGGTA TCATGGCTGG GTCAAACAGA TCTGGAGATC TGAAAGATGC CCAGAAGTCT 
ATTCCCATTG GGACCATCCT TGCCATCCTG ACCACATCCT TTGTGTATTT AAGCAATGTT 
GTCCTTTTTG GTGCATGTAT TGAAGGAGTC GTTCTCAGAG ACAAATTTGG GGATGCTGTA 
AAAGGGAATT TGGTTGTAGG CACCTTATCC TGGCCATCCC CGTGGGTGAT CGTTATTGGC 
TCCTTCTTTT CAACATGTGG GGCTGGGCTG CAGAGCCTCA CAGGTGCGCC TCGGCTGCTG 
CAGGCTATCG CCAAGGATAA CATCATACCT TTCCTTAGGG TTTTTGGTCA CAGCAAAGCT 
AATGGGGAAC CTACCTGGGC TTTACTTCTA ACTGCTGCCA TAGCAGAGCT GGGAATTCTC 
ATCGCCTCCC TGGATCTCGT GGCCCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC 
TTTGTGAACT TGGCTTGTGC CTTGCAAACA TTGCTGCGAA CCCCCAACTG GAGGCCTCGA 
TTCCGCTATT ATCACTGGGC CCTCTCTTTC ATGGGAATGA GTATCTGTCT AGCTCTGATG 
TTCATTTCTT CTTGGTATTA TGCCATTGTA GCTATGGTAA TAGCTGGCAT GATCTACAAG 
TACATTGAAT ATCAAGGGGC TGAGAAAGAA TGGGGGGATG GTATCCGTGG GCTGTCGCTC 
AGTGCAGCCC GCTTCGCTTT GCTCCGTCTA GAGGAAGGAC CTCCTCACAC TAAAAACTGG 
AGGCCTCAGC TGCTCGTCCT ACTGAAGCTG GATGAAGATT TACACGTCAA GCACCCTCGC 
CTCCTCACCT TTGCCTCCCA GCTCAAGGCA GGAAAGGGAC TCACGATTGT GGGCTCTGTC 
ATCGTGGGGA ACTTCTTGGA GAACTATGGT GACGCGCTCG CGGCAGAGCA GACCATTAAG 
CACCTAATGG AGGCAGAAAA GGTAAAAGGA TTCTGCCAAT TGGTGGTGGC TGCCAAGCTG 
AAAGAGGGCA TTTCACACCT CATCCAGTCC TGTGGCCTCG GAGGCATGAA ACACAACACA 
GTGGTGATGG GCTGGCCCAA TGGCTGGCGT CAGAGTGAAG ATGCTCGCGC TTGGAAGACT 
TTCATTGGCA CAGTACGAGT GACAACTGCT GCCCATCTAG CCCTGCTGGT GGCTAAAAAT 
GTCTCCTTCT TTCCCAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG 
ATTGTGCATG ATGGGGGCAT GCTCATGCTA TTACCGTTCC TGCTGAAACA GCACAAGGTT 
TGGCGGAAAT GCAGCATACG GATCTTCACA GTAGCCCAAC TAGAAGACAA CAGTATCCAG 
ATGAAGAAGG ATCTGGCCAC CTTTCTGTAC CACCTGCGCA TTGAGGCAGA AGTGGAAGTG 
GTGGAGATGC ACGACAGTGA CATATCTGCC TATACATATG AGCGCACCCT GATGATGGAG 
CAGAGGTCCC AGATGCTTCG GCATATGCGG CTGTCCAAAA CAGAGCGAGA CAGGGAGGCA 
CAGCTGGTGA AAGATCGAAA CTCAATGCTA CGCTTGACCA GCATTGGCTC TGATGAGGAC 
GAAGAGACAG AAACGTACCA GGAGAAGGTG CACATGACTT GGACCAAGGA TAAATACATG 
GCATCCCGGG GGCAAAAGGT CAAGTCAATG GAAGGATTCC AGGACCTACT TAATATGCGT 
CCGGACCAGT CCAACGTGAG ACGGATGCAT ACAGCAGTGA AGCTCAATGA AGTTATAGTC 
AACAAGTCTC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGACCACC CCGGAACCCT 
GAAGGTGATG AAAACTACAT GGAATTTCTA GAAGTGCTCA CTGAGGGATT AGAACGAGTC 
CTTCTTGTCC GGGGTGGTGG CAGTGAGGTC ATCACCATTT ACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349629.1
CDS	509..3916
Translation	MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSLATVALDPSSDRTSNPQDVTEDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	NM_001362700.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362700.1

ORF Insert Sequence:

ATGCATCCAC CAGAAGCCAC CACCAAGATG TCCTCAGTTC GGTTCATGGT GACACCAACT 
AAGATTGATG ACATTCCAGG TTTGTCAGAC ACCAGCCCGG ACCTCAGCTC TCGGTCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGCGAACTGT CTGGGGCTAC TACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ATCCCCAGGA TGTTACGGAG GATGACGGCC ATAAAAAAGC CCGAAATGCT 
TATCTCAATA ATTCCAACTA TGAAGAAGGA GACGAATATT TTGATAAAAA TTTGGCACTC 
TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTCAACCG CATGGCCAAC 
TATACAAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG 
AAAAAGAAGC CTACCAAGAG CCCCCAAATG GGTACTTTCA TGGGTGTCTA CCTCCCATGT 
CTACAGAACA TCTTTGGAGT GATCCTCTTC CTGCGTCTTA CCTGGGTAGT GGGAACAGCT 
GGAATCCTTC AGGCCTTTGC AATTGTCCTC ATCTGCTGCT GCTGTACAAT GTTAACTGCC 
ATCTCCATGA GCGCCATCGC CACTAACGGA GTGGTGCCAG CTGGGGGCTC ATACTTCATG 
ATTTCCAGAG CCCTGGGCCC AGAGTTTGGC GGGGCTGTAG GCCTCTGCTT TTATCTTGGC 
ACCACATTTG CAGCAGCCAT GTATATTCTT GGTGCCATTG AAATCTTTCT GGTATACATT 
GTCCCCCGAG CTGCCATCTT TCGGAGTGAC GATGCACTCA AGGAGTCAGC AGCTATGCTG 
AACAACATGC GCGTCTATGG TACAGCCTTC TTGGTCCTCA TGGTCTTGGT GGTATTCATC 
GGCGTACGCT ATGTGAATAA GTTTGCCTCA CTCTTCCTGG CCTGTGTAAT TGTGTCGATC 
TTGGCTATCT ATGCTGGTGC CATCAAGTCT TCCTTTGCTC CACCACACTT CCCGGTCTGT 
ATGCTGGGCA ACCGTACCCT GTCATCAAGA CACCTTGACA TTTGCTCTAA GACCAAGGAG 
GTTGACAACA TGACAGTACC ATCAAAGTTA TGGGGATTCT TCTGCAACTC GAGTCAGTTC 
TTTAATGCCA CCTGTGATGA GTACTTTGTT CACAATAACG TCATCTCAAT CCAAGGCATT 
CCAGGGTTGG CTAGTGGTAT CATTACTGAA AATCTTTGGA GTAATTATTT ACCAAAGGGT 
GAGATAATTG AAAAGCCATC AGCCAAGTCA TCTGATGTCT TAGGCAACTT AAACCATGAA 
TATGTTCTTG CTGATATCAC CACCTCCTTC ACTCTGCTGG TGGGGATCTT CTTTCCCTCG 
GTCACAGGTA TCATGGCTGG GTCAAACAGA TCTGGAGATC TGAAAGATGC CCAGAAGTCT 
ATTCCCATTG GGACCATCCT TGCCATCCTG ACCACATCCT TTGTGTATTT AAGCAATGTT 
GTCCTTTTTG GTGCATGTAT TGAAGGAGTC GTTCTCAGAG ACAAATTTGG GGATGCTGTA 
AAAGGGAATT TGGTTGTAGG CACCTTATCC TGGCCATCCC CGTGGGTGAT CGTTATTGGC 
TCCTTCTTTT CAACATGTGG GGCTGGGCTG CAGAGCCTCA CAGGTGCGCC TCGGCTGCTG 
CAGGCTATCG CCAAGGATAA CATCATACCT TTCCTTAGGG TTTTTGGTCA CAGCAAAGCT 
AATGGGGAAC CTACCTGGGC TTTACTTCTA ACTGCTGCCA TAGCAGAGCT GGGAATTCTC 
ATCGCCTCCC TGGATCTCGT GGCCCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC 
TTTGTGAACT TGGCTTGTGC CTTGCAAACA TTGCTGCGAA CCCCCAACTG GAGGCCTCGA 
TTCCGCTATT ATCACTGGGC CCTCTCTTTC ATGGGAATGA GTATCTGTCT AGCTCTGATG 
TTCATTTCTT CTTGGTATTA TGCCATTGTA GCTATGGTAA TAGCTGGCAT GATCTACAAG 
TACATTGAAT ATCAAGGGGC TGAGAAAGAA TGGGGGGATG GTATCCGTGG GCTGTCGCTC 
AGTGCAGCCC GCTTCGCTTT GCTCCGTCTA GAGGAAGGAC CTCCTCACAC TAAAAACTGG 
AGGCCTCAGC TGCTCGTCCT ACTGAAGCTG GATGAAGATT TACACGTCAA GCACCCTCGC 
CTCCTCACCT TTGCCTCCCA GCTCAAGGCA GGAAAGGGAC TCACGATTGT GGGCTCTGTC 
ATCGTGGGGA ACTTCTTGGA GAACTATGGT GACGCGCTCG CGGCAGAGCA GACCATTAAG 
CACCTAATGG AGGCAGAAAA GGTAAAAGGA TTCTGCCAAT TGGTGGTGGC TGCCAAGCTG 
AAAGAGGGCA TTTCACACCT CATCCAGTCC TGTGGCCTCG GAGGCATGAA ACACAACACA 
GTGGTGATGG GCTGGCCCAA TGGCTGGCGT CAGAGTGAAG ATGCTCGCGC TTGGAAGACT 
TTCATTGGCA CAGTACGAGT GACAACTGCT GCCCATCTAG CCCTGCTGGT GGCTAAAAAT 
GTCTCCTTCT TTCCCAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG 
ATTGTGCATG ATGGGGGCAT GCTCATGCTA TTACCGTTCC TGCTGAAACA GCACAAGGTT 
TGGCGGAAAT GCAGCATACG GATCTTCACA GTAGCCCAAC TAGAAGACAA CAGTATCCAG 
ATGAAGAAGG ATCTGGCCAC CTTTCTGTAC CACCTGCGCA TTGAGGCAGA AGTGGAAGTG 
GTGGAGATGC ACGACAGTGA CATATCTGCC TATACATATG AGCGCACCCT GATGATGGAG 
CAGAGGTCCC AGATGCTTCG GCATATGCGG CTGTCCAAAA CAGAGCGAGA CAGGGAGGCA 
CAGCTGGTGA AAGATCGAAA CTCAATGCTA CGCTTGACCA GCATTGGCTC TGATGAGGAC 
GAAGAGACAG AAACGTACCA GGAGAAGGTG CACATGACTT GGACCAAGGA TAAATACATG 
GCATCCCGGG GGCAAAAGGT CAAGTCAATG GAAGGATTCC AGGACCTACT TAATATGCGT 
CCGGACCAGT CCAACGTGAG ACGGATGCAT ACAGCAGTGA AGCTCAATGA AGTTATAGTC 
AACAAGTCTC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGACCACC CCGGAACCCT 
GAAGGTGATG AAAACTACAT GGAATTTCTA GAAGTGCTCA CTGAGGGATT AGAACGAGTC 
CTTCTTGTCC GGGGTGGTGG CAGTGAGGTC ATCACCATTT ACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a6 ( XM_006498545.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498608.1 Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.

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CloneID	OMu29896
Clone ID Related Accession (Same CDS sequence)	NM_001362700.1 , XM_006498545.3
Accession Version	XM_006498545.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498545.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCCAC CAGAAGCCAC CACCAAGATG TCCTCAGTTC GGTTCATGGT GACACCAACT 
AAGATTGATG ACATTCCAGG TTTGTCAGAC ACCAGCCCGG ACCTCAGCTC TCGGTCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGCGAACTGT CTGGGGCTAC TACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ATCCCCAGGA TGTTACGGAG GATGACGGCC ATAAAAAAGC CCGAAATGCT 
TATCTCAATA ATTCCAACTA TGAAGAAGGA GACGAATATT TTGATAAAAA TTTGGCACTC 
TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTCAACCG CATGGCCAAC 
TATACAAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG 
AAAAAGAAGC CTACCAAGAG CCCCCAAATG GGTACTTTCA TGGGTGTCTA CCTCCCATGT 
CTACAGAACA TCTTTGGAGT GATCCTCTTC CTGCGTCTTA CCTGGGTAGT GGGAACAGCT 
GGAATCCTTC AGGCCTTTGC AATTGTCCTC ATCTGCTGCT GCTGTACAAT GTTAACTGCC 
ATCTCCATGA GCGCCATCGC CACTAACGGA GTGGTGCCAG CTGGGGGCTC ATACTTCATG 
ATTTCCAGAG CCCTGGGCCC AGAGTTTGGC GGGGCTGTAG GCCTCTGCTT TTATCTTGGC 
ACCACATTTG CAGCAGCCAT GTATATTCTT GGTGCCATTG AAATCTTTCT GGTATACATT 
GTCCCCCGAG CTGCCATCTT TCGGAGTGAC GATGCACTCA AGGAGTCAGC AGCTATGCTG 
AACAACATGC GCGTCTATGG TACAGCCTTC TTGGTCCTCA TGGTCTTGGT GGTATTCATC 
GGCGTACGCT ATGTGAATAA GTTTGCCTCA CTCTTCCTGG CCTGTGTAAT TGTGTCGATC 
TTGGCTATCT ATGCTGGTGC CATCAAGTCT TCCTTTGCTC CACCACACTT CCCGGTCTGT 
ATGCTGGGCA ACCGTACCCT GTCATCAAGA CACCTTGACA TTTGCTCTAA GACCAAGGAG 
GTTGACAACA TGACAGTACC ATCAAAGTTA TGGGGATTCT TCTGCAACTC GAGTCAGTTC 
TTTAATGCCA CCTGTGATGA GTACTTTGTT CACAATAACG TCATCTCAAT CCAAGGCATT 
CCAGGGTTGG CTAGTGGTAT CATTACTGAA AATCTTTGGA GTAATTATTT ACCAAAGGGT 
GAGATAATTG AAAAGCCATC AGCCAAGTCA TCTGATGTCT TAGGCAACTT AAACCATGAA 
TATGTTCTTG CTGATATCAC CACCTCCTTC ACTCTGCTGG TGGGGATCTT CTTTCCCTCG 
GTCACAGGTA TCATGGCTGG GTCAAACAGA TCTGGAGATC TGAAAGATGC CCAGAAGTCT 
ATTCCCATTG GGACCATCCT TGCCATCCTG ACCACATCCT TTGTGTATTT AAGCAATGTT 
GTCCTTTTTG GTGCATGTAT TGAAGGAGTC GTTCTCAGAG ACAAATTTGG GGATGCTGTA 
AAAGGGAATT TGGTTGTAGG CACCTTATCC TGGCCATCCC CGTGGGTGAT CGTTATTGGC 
TCCTTCTTTT CAACATGTGG GGCTGGGCTG CAGAGCCTCA CAGGTGCGCC TCGGCTGCTG 
CAGGCTATCG CCAAGGATAA CATCATACCT TTCCTTAGGG TTTTTGGTCA CAGCAAAGCT 
AATGGGGAAC CTACCTGGGC TTTACTTCTA ACTGCTGCCA TAGCAGAGCT GGGAATTCTC 
ATCGCCTCCC TGGATCTCGT GGCCCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC 
TTTGTGAACT TGGCTTGTGC CTTGCAAACA TTGCTGCGAA CCCCCAACTG GAGGCCTCGA 
TTCCGCTATT ATCACTGGGC CCTCTCTTTC ATGGGAATGA GTATCTGTCT AGCTCTGATG 
TTCATTTCTT CTTGGTATTA TGCCATTGTA GCTATGGTAA TAGCTGGCAT GATCTACAAG 
TACATTGAAT ATCAAGGGGC TGAGAAAGAA TGGGGGGATG GTATCCGTGG GCTGTCGCTC 
AGTGCAGCCC GCTTCGCTTT GCTCCGTCTA GAGGAAGGAC CTCCTCACAC TAAAAACTGG 
AGGCCTCAGC TGCTCGTCCT ACTGAAGCTG GATGAAGATT TACACGTCAA GCACCCTCGC 
CTCCTCACCT TTGCCTCCCA GCTCAAGGCA GGAAAGGGAC TCACGATTGT GGGCTCTGTC 
ATCGTGGGGA ACTTCTTGGA GAACTATGGT GACGCGCTCG CGGCAGAGCA GACCATTAAG 
CACCTAATGG AGGCAGAAAA GGTAAAAGGA TTCTGCCAAT TGGTGGTGGC TGCCAAGCTG 
AAAGAGGGCA TTTCACACCT CATCCAGTCC TGTGGCCTCG GAGGCATGAA ACACAACACA 
GTGGTGATGG GCTGGCCCAA TGGCTGGCGT CAGAGTGAAG ATGCTCGCGC TTGGAAGACT 
TTCATTGGCA CAGTACGAGT GACAACTGCT GCCCATCTAG CCCTGCTGGT GGCTAAAAAT 
GTCTCCTTCT TTCCCAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG 
ATTGTGCATG ATGGGGGCAT GCTCATGCTA TTACCGTTCC TGCTGAAACA GCACAAGGTT 
TGGCGGAAAT GCAGCATACG GATCTTCACA GTAGCCCAAC TAGAAGACAA CAGTATCCAG 
ATGAAGAAGG ATCTGGCCAC CTTTCTGTAC CACCTGCGCA TTGAGGCAGA AGTGGAAGTG 
GTGGAGATGC ACGACAGTGA CATATCTGCC TATACATATG AGCGCACCCT GATGATGGAG 
CAGAGGTCCC AGATGCTTCG GCATATGCGG CTGTCCAAAA CAGAGCGAGA CAGGGAGGCA 
CAGCTGGTGA AAGATCGAAA CTCAATGCTA CGCTTGACCA GCATTGGCTC TGATGAGGAC 
GAAGAGACAG AAACGTACCA GGAGAAGGTG CACATGACTT GGACCAAGGA TAAATACATG 
GCATCCCGGG GGCAAAAGGT CAAGTCAATG GAAGGATTCC AGGACCTACT TAATATGCGT 
CCGGACCAGT CCAACGTGAG ACGGATGCAT ACAGCAGTGA AGCTCAATGA AGTTATAGTC 
AACAAGTCTC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGACCACC CCGGAACCCT 
GAAGGTGATG AAAACTACAT GGAATTTCTA GAAGTGCTCA CTGAGGGATT AGAACGAGTC 
CTTCTTGTCC GGGGTGGTGG CAGTGAGGTC ATCACCATTT ACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498608.1
CDS	781..4188
Translation	MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSLATVALDPSSDRTSNPQDVTEDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	XM_006498545.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498545.3

ORF Insert Sequence:

ATGCATCCAC CAGAAGCCAC CACCAAGATG TCCTCAGTTC GGTTCATGGT GACACCAACT 
AAGATTGATG ACATTCCAGG TTTGTCAGAC ACCAGCCCGG ACCTCAGCTC TCGGTCTAGT 
TCTCGAGTAA GATTTAGCTC CCGAGAAAGT GTGCCAGAAA CAAGCCGTAG TGAGCCTATG 
AGCGAACTGT CTGGGGCTAC TACTTCTCTG GCAACTGTTG CCCTAGATCC TTCCAGTGAC 
CGGACTTCTA ATCCCCAGGA TGTTACGGAG GATGACGGCC ATAAAAAAGC CCGAAATGCT 
TATCTCAATA ATTCCAACTA TGAAGAAGGA GACGAATATT TTGATAAAAA TTTGGCACTC 
TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTCAACCG CATGGCCAAC 
TATACAAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG 
AAAAAGAAGC CTACCAAGAG CCCCCAAATG GGTACTTTCA TGGGTGTCTA CCTCCCATGT 
CTACAGAACA TCTTTGGAGT GATCCTCTTC CTGCGTCTTA CCTGGGTAGT GGGAACAGCT 
GGAATCCTTC AGGCCTTTGC AATTGTCCTC ATCTGCTGCT GCTGTACAAT GTTAACTGCC 
ATCTCCATGA GCGCCATCGC CACTAACGGA GTGGTGCCAG CTGGGGGCTC ATACTTCATG 
ATTTCCAGAG CCCTGGGCCC AGAGTTTGGC GGGGCTGTAG GCCTCTGCTT TTATCTTGGC 
ACCACATTTG CAGCAGCCAT GTATATTCTT GGTGCCATTG AAATCTTTCT GGTATACATT 
GTCCCCCGAG CTGCCATCTT TCGGAGTGAC GATGCACTCA AGGAGTCAGC AGCTATGCTG 
AACAACATGC GCGTCTATGG TACAGCCTTC TTGGTCCTCA TGGTCTTGGT GGTATTCATC 
GGCGTACGCT ATGTGAATAA GTTTGCCTCA CTCTTCCTGG CCTGTGTAAT TGTGTCGATC 
TTGGCTATCT ATGCTGGTGC CATCAAGTCT TCCTTTGCTC CACCACACTT CCCGGTCTGT 
ATGCTGGGCA ACCGTACCCT GTCATCAAGA CACCTTGACA TTTGCTCTAA GACCAAGGAG 
GTTGACAACA TGACAGTACC ATCAAAGTTA TGGGGATTCT TCTGCAACTC GAGTCAGTTC 
TTTAATGCCA CCTGTGATGA GTACTTTGTT CACAATAACG TCATCTCAAT CCAAGGCATT 
CCAGGGTTGG CTAGTGGTAT CATTACTGAA AATCTTTGGA GTAATTATTT ACCAAAGGGT 
GAGATAATTG AAAAGCCATC AGCCAAGTCA TCTGATGTCT TAGGCAACTT AAACCATGAA 
TATGTTCTTG CTGATATCAC CACCTCCTTC ACTCTGCTGG TGGGGATCTT CTTTCCCTCG 
GTCACAGGTA TCATGGCTGG GTCAAACAGA TCTGGAGATC TGAAAGATGC CCAGAAGTCT 
ATTCCCATTG GGACCATCCT TGCCATCCTG ACCACATCCT TTGTGTATTT AAGCAATGTT 
GTCCTTTTTG GTGCATGTAT TGAAGGAGTC GTTCTCAGAG ACAAATTTGG GGATGCTGTA 
AAAGGGAATT TGGTTGTAGG CACCTTATCC TGGCCATCCC CGTGGGTGAT CGTTATTGGC 
TCCTTCTTTT CAACATGTGG GGCTGGGCTG CAGAGCCTCA CAGGTGCGCC TCGGCTGCTG 
CAGGCTATCG CCAAGGATAA CATCATACCT TTCCTTAGGG TTTTTGGTCA CAGCAAAGCT 
AATGGGGAAC CTACCTGGGC TTTACTTCTA ACTGCTGCCA TAGCAGAGCT GGGAATTCTC 
ATCGCCTCCC TGGATCTCGT GGCCCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC 
TTTGTGAACT TGGCTTGTGC CTTGCAAACA TTGCTGCGAA CCCCCAACTG GAGGCCTCGA 
TTCCGCTATT ATCACTGGGC CCTCTCTTTC ATGGGAATGA GTATCTGTCT AGCTCTGATG 
TTCATTTCTT CTTGGTATTA TGCCATTGTA GCTATGGTAA TAGCTGGCAT GATCTACAAG 
TACATTGAAT ATCAAGGGGC TGAGAAAGAA TGGGGGGATG GTATCCGTGG GCTGTCGCTC 
AGTGCAGCCC GCTTCGCTTT GCTCCGTCTA GAGGAAGGAC CTCCTCACAC TAAAAACTGG 
AGGCCTCAGC TGCTCGTCCT ACTGAAGCTG GATGAAGATT TACACGTCAA GCACCCTCGC 
CTCCTCACCT TTGCCTCCCA GCTCAAGGCA GGAAAGGGAC TCACGATTGT GGGCTCTGTC 
ATCGTGGGGA ACTTCTTGGA GAACTATGGT GACGCGCTCG CGGCAGAGCA GACCATTAAG 
CACCTAATGG AGGCAGAAAA GGTAAAAGGA TTCTGCCAAT TGGTGGTGGC TGCCAAGCTG 
AAAGAGGGCA TTTCACACCT CATCCAGTCC TGTGGCCTCG GAGGCATGAA ACACAACACA 
GTGGTGATGG GCTGGCCCAA TGGCTGGCGT CAGAGTGAAG ATGCTCGCGC TTGGAAGACT 
TTCATTGGCA CAGTACGAGT GACAACTGCT GCCCATCTAG CCCTGCTGGT GGCTAAAAAT 
GTCTCCTTCT TTCCCAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG 
ATTGTGCATG ATGGGGGCAT GCTCATGCTA TTACCGTTCC TGCTGAAACA GCACAAGGTT 
TGGCGGAAAT GCAGCATACG GATCTTCACA GTAGCCCAAC TAGAAGACAA CAGTATCCAG 
ATGAAGAAGG ATCTGGCCAC CTTTCTGTAC CACCTGCGCA TTGAGGCAGA AGTGGAAGTG 
GTGGAGATGC ACGACAGTGA CATATCTGCC TATACATATG AGCGCACCCT GATGATGGAG 
CAGAGGTCCC AGATGCTTCG GCATATGCGG CTGTCCAAAA CAGAGCGAGA CAGGGAGGCA 
CAGCTGGTGA AAGATCGAAA CTCAATGCTA CGCTTGACCA GCATTGGCTC TGATGAGGAC 
GAAGAGACAG AAACGTACCA GGAGAAGGTG CACATGACTT GGACCAAGGA TAAATACATG 
GCATCCCGGG GGCAAAAGGT CAAGTCAATG GAAGGATTCC AGGACCTACT TAATATGCGT 
CCGGACCAGT CCAACGTGAG ACGGATGCAT ACAGCAGTGA AGCTCAATGA AGTTATAGTC 
AACAAGTCTC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGACCACC CCGGAACCCT 
GAAGGTGATG AAAACTACAT GGAATTTCTA GAAGTGCTCA CTGAGGGATT AGAACGAGTC 
CTTCTTGTCC GGGGTGGTGG CAGTGAGGTC ATCACCATTT ACTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a6 ( XM_006498546.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498609.1 Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X2, mRNA.

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CloneID	OMu29897
Clone ID Related Accession (Same CDS sequence)	XM_006498546.3 , XM_006498546.4
Accession Version	XM_006498546.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	solute carrier family 12 member 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCACT TCACTGTGAC CAAGGTAGAA GACCCAGAGG AGGGGGCAGC TGGCCCCCTC 
TCTCCTGAGC CCAGCTCAGC AGAAGTAAAA GCCCGGATTC AGGATCCCCA AGAACCAGAT 
GACGGCCATA AAAAAGCCCG AAATGCTTAT CTCAATAATT CCAACTATGA AGAAGGAGAC 
GAATATTTTG ATAAAAATTT GGCACTCTTT GAGGAAGAAA TGGACACCAG ACCAAAGGTG 
TCTTCTCTCC TCAACCGCAT GGCCAACTAT ACAAATCTGA CACAAGGAGC AAAGGAACAT 
GAAGAGGCAG AGAACATCAC TGAAGGGAAA AAGAAGCCTA CCAAGAGCCC CCAAATGGGT 
ACTTTCATGG GTGTCTACCT CCCATGTCTA CAGAACATCT TTGGAGTGAT CCTCTTCCTG 
CGTCTTACCT GGGTAGTGGG AACAGCTGGA ATCCTTCAGG CCTTTGCAAT TGTCCTCATC 
TGCTGCTGCT GTACAATGTT AACTGCCATC TCCATGAGCG CCATCGCCAC TAACGGAGTG 
GTGCCAGCTG GGGGCTCATA CTTCATGATT TCCAGAGCCC TGGGCCCAGA GTTTGGCGGG 
GCTGTAGGCC TCTGCTTTTA TCTTGGCACC ACATTTGCAG CAGCCATGTA TATTCTTGGT 
GCCATTGAAA TCTTTCTGGT ATACATTGTC CCCCGAGCTG CCATCTTTCG GAGTGACGAT 
GCACTCAAGG AGTCAGCAGC TATGCTGAAC AACATGCGCG TCTATGGTAC AGCCTTCTTG 
GTCCTCATGG TCTTGGTGGT ATTCATCGGC GTACGCTATG TGAATAAGTT TGCCTCACTC 
TTCCTGGCCT GTGTAATTGT GTCGATCTTG GCTATCTATG CTGGTGCCAT CAAGTCTTCC 
TTTGCTCCAC CACACTTCCC GGTCTGTATG CTGGGCAACC GTACCCTGTC ATCAAGACAC 
CTTGACATTT GCTCTAAGAC CAAGGAGGTT GACAACATGA CAGTACCATC AAAGTTATGG 
GGATTCTTCT GCAACTCGAG TCAGTTCTTT AATGCCACCT GTGATGAGTA CTTTGTTCAC 
AATAACGTCA TCTCAATCCA AGGCATTCCA GGGTTGGCTA GTGGTATCAT TACTGAAAAT 
CTTTGGAGTA ATTATTTACC AAAGGGTGAG ATAATTGAAA AGCCATCAGC CAAGTCATCT 
GATGTCTTAG GCAACTTAAA CCATGAATAT GTTCTTGCTG ATATCACCAC CTCCTTCACT 
CTGCTGGTGG GGATCTTCTT TCCCTCGGTC ACAGGTATCA TGGCTGGGTC AAACAGATCT 
GGAGATCTGA AAGATGCCCA GAAGTCTATT CCCATTGGGA CCATCCTTGC CATCCTGACC 
ACATCCTTTG TGTATTTAAG CAATGTTGTC CTTTTTGGTG CATGTATTGA AGGAGTCGTT 
CTCAGAGACA AATTTGGGGA TGCTGTAAAA GGGAATTTGG TTGTAGGCAC CTTATCCTGG 
CCATCCCCGT GGGTGATCGT TATTGGCTCC TTCTTTTCAA CATGTGGGGC TGGGCTGCAG 
AGCCTCACAG GTGCGCCTCG GCTGCTGCAG GCTATCGCCA AGGATAACAT CATACCTTTC 
CTTAGGGTTT TTGGTCACAG CAAAGCTAAT GGGGAACCTA CCTGGGCTTT ACTTCTAACT 
GCTGCCATAG CAGAGCTGGG AATTCTCATC GCCTCCCTGG ATCTCGTGGC CCCAATTCTT 
TCCATGTTTT TTCTCATGTG TTACCTCTTT GTGAACTTGG CTTGTGCCTT GCAAACATTG 
CTGCGAACCC CCAACTGGAG GCCTCGATTC CGCTATTATC ACTGGGCCCT CTCTTTCATG 
GGAATGAGTA TCTGTCTAGC TCTGATGTTC ATTTCTTCTT GGTATTATGC CATTGTAGCT 
ATGGTAATAG CTGGCATGAT CTACAAGTAC ATTGAATATC AAGGGGCTGA GAAAGAATGG 
GGGGATGGTA TCCGTGGGCT GTCGCTCAGT GCAGCCCGCT TCGCTTTGCT CCGTCTAGAG 
GAAGGACCTC CTCACACTAA AAACTGGAGG CCTCAGCTGC TCGTCCTACT GAAGCTGGAT 
GAAGATTTAC ACGTCAAGCA CCCTCGCCTC CTCACCTTTG CCTCCCAGCT CAAGGCAGGA 
AAGGGACTCA CGATTGTGGG CTCTGTCATC GTGGGGAACT TCTTGGAGAA CTATGGTGAC 
GCGCTCGCGG CAGAGCAGAC CATTAAGCAC CTAATGGAGG CAGAAAAGGT AAAAGGATTC 
TGCCAATTGG TGGTGGCTGC CAAGCTGAAA GAGGGCATTT CACACCTCAT CCAGTCCTGT 
GGCCTCGGAG GCATGAAACA CAACACAGTG GTGATGGGCT GGCCCAATGG CTGGCGTCAG 
AGTGAAGATG CTCGCGCTTG GAAGACTTTC ATTGGCACAG TACGAGTGAC AACTGCTGCC 
CATCTAGCCC TGCTGGTGGC TAAAAATGTC TCCTTCTTTC CCAGCAATGT GGAGCAGTTT 
TCTGAGGGCA ACATTGATGT GTGGTGGATT GTGCATGATG GGGGCATGCT CATGCTATTA 
CCGTTCCTGC TGAAACAGCA CAAGGTTTGG CGGAAATGCA GCATACGGAT CTTCACAGTA 
GCCCAACTAG AAGACAACAG TATCCAGATG AAGAAGGATC TGGCCACCTT TCTGTACCAC 
CTGCGCATTG AGGCAGAAGT GGAAGTGGTG GAGATGCACG ACAGTGACAT ATCTGCCTAT 
ACATATGAGC GCACCCTGAT GATGGAGCAG AGGTCCCAGA TGCTTCGGCA TATGCGGCTG 
TCCAAAACAG AGCGAGACAG GGAGGCACAG CTGGTGAAAG ATCGAAACTC AATGCTACGC 
TTGACCAGCA TTGGCTCTGA TGAGGACGAA GAGACAGAAA CGTACCAGGA GAAGGTGCAC 
ATGACTTGGA CCAAGGATAA ATACATGGCA TCCCGGGGGC AAAAGGTCAA GTCAATGGAA 
GGATTCCAGG ACCTACTTAA TATGCGTCCG GACCAGTCCA ACGTGAGACG GATGCATACA 
GCAGTGAAGC TCAATGAAGT TATAGTCAAC AAGTCTCATG AAGCAAAGCT GGTTTTGTTG 
AATATGCCAG GACCACCCCG GAACCCTGAA GGTGATGAAA ACTACATGGA ATTTCTAGAA 
GTGCTCACTG AGGGATTAGA ACGAGTCCTT CTTGTCCGGG GTGGTGGCAG TGAGGTCATC 
ACCATTTACT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498609.1
CDS	274..3528
Translation	MPHFTVTKVEDPEEGAAGPLSPEPSSAEVKARIQDPQEPDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMANYTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVLICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGIPGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	XM_006498546.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus solute carrier family 12, member 6 (Slc12a6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498546.3

ORF Insert Sequence:

ATGCCGCACT TCACTGTGAC CAAGGTAGAA GACCCAGAGG AGGGGGCAGC TGGCCCCCTC 
TCTCCTGAGC CCAGCTCAGC AGAAGTAAAA GCCCGGATTC AGGATCCCCA AGAACCAGAT 
GACGGCCATA AAAAAGCCCG AAATGCTTAT CTCAATAATT CCAACTATGA AGAAGGAGAC 
GAATATTTTG ATAAAAATTT GGCACTCTTT GAGGAAGAAA TGGACACCAG ACCAAAGGTG 
TCTTCTCTCC TCAACCGCAT GGCCAACTAT ACAAATCTGA CACAAGGAGC AAAGGAACAT 
GAAGAGGCAG AGAACATCAC TGAAGGGAAA AAGAAGCCTA CCAAGAGCCC CCAAATGGGT 
ACTTTCATGG GTGTCTACCT CCCATGTCTA CAGAACATCT TTGGAGTGAT CCTCTTCCTG 
CGTCTTACCT GGGTAGTGGG AACAGCTGGA ATCCTTCAGG CCTTTGCAAT TGTCCTCATC 
TGCTGCTGCT GTACAATGTT AACTGCCATC TCCATGAGCG CCATCGCCAC TAACGGAGTG 
GTGCCAGCTG GGGGCTCATA CTTCATGATT TCCAGAGCCC TGGGCCCAGA GTTTGGCGGG 
GCTGTAGGCC TCTGCTTTTA TCTTGGCACC ACATTTGCAG CAGCCATGTA TATTCTTGGT 
GCCATTGAAA TCTTTCTGGT ATACATTGTC CCCCGAGCTG CCATCTTTCG GAGTGACGAT 
GCACTCAAGG AGTCAGCAGC TATGCTGAAC AACATGCGCG TCTATGGTAC AGCCTTCTTG 
GTCCTCATGG TCTTGGTGGT ATTCATCGGC GTACGCTATG TGAATAAGTT TGCCTCACTC 
TTCCTGGCCT GTGTAATTGT GTCGATCTTG GCTATCTATG CTGGTGCCAT CAAGTCTTCC 
TTTGCTCCAC CACACTTCCC GGTCTGTATG CTGGGCAACC GTACCCTGTC ATCAAGACAC 
CTTGACATTT GCTCTAAGAC CAAGGAGGTT GACAACATGA CAGTACCATC AAAGTTATGG 
GGATTCTTCT GCAACTCGAG TCAGTTCTTT AATGCCACCT GTGATGAGTA CTTTGTTCAC 
AATAACGTCA TCTCAATCCA AGGCATTCCA GGGTTGGCTA GTGGTATCAT TACTGAAAAT 
CTTTGGAGTA ATTATTTACC AAAGGGTGAG ATAATTGAAA AGCCATCAGC CAAGTCATCT 
GATGTCTTAG GCAACTTAAA CCATGAATAT GTTCTTGCTG ATATCACCAC CTCCTTCACT 
CTGCTGGTGG GGATCTTCTT TCCCTCGGTC ACAGGTATCA TGGCTGGGTC AAACAGATCT 
GGAGATCTGA AAGATGCCCA GAAGTCTATT CCCATTGGGA CCATCCTTGC CATCCTGACC 
ACATCCTTTG TGTATTTAAG CAATGTTGTC CTTTTTGGTG CATGTATTGA AGGAGTCGTT 
CTCAGAGACA AATTTGGGGA TGCTGTAAAA GGGAATTTGG TTGTAGGCAC CTTATCCTGG 
CCATCCCCGT GGGTGATCGT TATTGGCTCC TTCTTTTCAA CATGTGGGGC TGGGCTGCAG 
AGCCTCACAG GTGCGCCTCG GCTGCTGCAG GCTATCGCCA AGGATAACAT CATACCTTTC 
CTTAGGGTTT TTGGTCACAG CAAAGCTAAT GGGGAACCTA CCTGGGCTTT ACTTCTAACT 
GCTGCCATAG CAGAGCTGGG AATTCTCATC GCCTCCCTGG ATCTCGTGGC CCCAATTCTT 
TCCATGTTTT TTCTCATGTG TTACCTCTTT GTGAACTTGG CTTGTGCCTT GCAAACATTG 
CTGCGAACCC CCAACTGGAG GCCTCGATTC CGCTATTATC ACTGGGCCCT CTCTTTCATG 
GGAATGAGTA TCTGTCTAGC TCTGATGTTC ATTTCTTCTT GGTATTATGC CATTGTAGCT 
ATGGTAATAG CTGGCATGAT CTACAAGTAC ATTGAATATC AAGGGGCTGA GAAAGAATGG 
GGGGATGGTA TCCGTGGGCT GTCGCTCAGT GCAGCCCGCT TCGCTTTGCT CCGTCTAGAG 
GAAGGACCTC CTCACACTAA AAACTGGAGG CCTCAGCTGC TCGTCCTACT GAAGCTGGAT 
GAAGATTTAC ACGTCAAGCA CCCTCGCCTC CTCACCTTTG CCTCCCAGCT CAAGGCAGGA 
AAGGGACTCA CGATTGTGGG CTCTGTCATC GTGGGGAACT TCTTGGAGAA CTATGGTGAC 
GCGCTCGCGG CAGAGCAGAC CATTAAGCAC CTAATGGAGG CAGAAAAGGT AAAAGGATTC 
TGCCAATTGG TGGTGGCTGC CAAGCTGAAA GAGGGCATTT CACACCTCAT CCAGTCCTGT 
GGCCTCGGAG GCATGAAACA CAACACAGTG GTGATGGGCT GGCCCAATGG CTGGCGTCAG 
AGTGAAGATG CTCGCGCTTG GAAGACTTTC ATTGGCACAG TACGAGTGAC AACTGCTGCC 
CATCTAGCCC TGCTGGTGGC TAAAAATGTC TCCTTCTTTC CCAGCAATGT GGAGCAGTTT 
TCTGAGGGCA ACATTGATGT GTGGTGGATT GTGCATGATG GGGGCATGCT CATGCTATTA 
CCGTTCCTGC TGAAACAGCA CAAGGTTTGG CGGAAATGCA GCATACGGAT CTTCACAGTA 
GCCCAACTAG AAGACAACAG TATCCAGATG AAGAAGGATC TGGCCACCTT TCTGTACCAC 
CTGCGCATTG AGGCAGAAGT GGAAGTGGTG GAGATGCACG ACAGTGACAT ATCTGCCTAT 
ACATATGAGC GCACCCTGAT GATGGAGCAG AGGTCCCAGA TGCTTCGGCA TATGCGGCTG 
TCCAAAACAG AGCGAGACAG GGAGGCACAG CTGGTGAAAG ATCGAAACTC AATGCTACGC 
TTGACCAGCA TTGGCTCTGA TGAGGACGAA GAGACAGAAA CGTACCAGGA GAAGGTGCAC 
ATGACTTGGA CCAAGGATAA ATACATGGCA TCCCGGGGGC AAAAGGTCAA GTCAATGGAA 
GGATTCCAGG ACCTACTTAA TATGCGTCCG GACCAGTCCA ACGTGAGACG GATGCATACA 
GCAGTGAAGC TCAATGAAGT TATAGTCAAC AAGTCTCATG AAGCAAAGCT GGTTTTGTTG 
AATATGCCAG GACCACCCCG GAACCCTGAA GGTGATGAAA ACTACATGGA ATTTCTAGAA 
GTGCTCACTG AGGGATTAGA ACGAGTCCTT CTTGTCCGGG GTGGTGGCAG TGAGGTCATC 
ACCATTTACT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KCC3 loss-of-function contributes to Andermann syndrome by inducing activity-dependent neuromuscular junction defects.
Neurobiology of disease10635-48(2017 Oct)
Bowerman M,Salsac C,Bernard V,Soulard C,Dionne A,Coque E,Benlefki S,Hince P,Dion PA,Butler-Browne G,Camu W,Bouchard JP,Delpire E,Rouleau GA,Raoul C,Scamps F

KCC3 deficiency-induced disruption of paranodal loops and impairment of axonal excitability in the peripheral nervous system.
Neuroscience33591-102(2016 Oct)
Sun YT,Tzeng SF,Lin TS,Hsu KS,Delpire E,Shen MR

Functional kinomics establishes a critical node of volume-sensitive cation-Cl
Scientific reports635986(2016 Oct)
Zhang J,Gao G,Begum G,Wang J,Khanna AR,Shmukler BE,Daubner GM,de Los Heros P,Davies P,Varghese J,Bhuiyan MI,Duan J,Zhang J,Duran D,Alper SL,Sun D,Elledge SJ,Alessi DR,Kahle KT

Peripheral motor neuropathy is associated with defective kinase regulation of the KCC3 cotransporter.
Science signaling9(439)ra77(2016 Aug)
Kahle KT,Flores B,Bharucha-Goebel D,Zhang J,Donkervoort S,Hegde M,Hussain G,Duran D,Liang B,Sun D,B?nnemann CG,Delpire E

Ablation of Potassium-Chloride Cotransporter Type 3 (Kcc3) in Mouse Causes Multiple Cardiovascular Defects and Isosmotic Polyuria.
PloS one11(5)e0154398(2016)
Garneau AP,Marcoux AA,No?l M,Frenette-Cotton R,Drolet MC,Couet J,Larivi?re R,Isenring P

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Slc12a6 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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