SLC12A6 cDNA ORF clone, Bos taurus(cattle)

The following SLC12A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa91641 XM_015473086.1
Latest version!
Bos taurus solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91642 XM_024997562.1
Latest version!
Bos taurus solute carrier family 12 member 6 (SLC12A6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa91642 XM_015473087.2
Latest version!
Bos taurus solute carrier family 12 member 6 (SLC12A6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa37403 NM_001193116.1
Latest version!
Bos taurus solute carrier family 12 member 6 (SLC12A6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23092 XM_005211495.4
Latest version!
Bos taurus solute carrier family 12 member 6 (SLC12A6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa23094 XM_005211497.4
Latest version!
Bos taurus solute carrier family 12 member 6 (SLC12A6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa23094 XM_005211497.3 Bos taurus solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa91642 XM_015473087.1 Bos taurus solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa23092 XM_005211495.3 Bos taurus solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa91641
    Clone ID Related Accession (Same CDS sequence) XM_015473086.1
    Accession Version XM_015473086.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACACT TCACAGTGAC TAAAGTAGAG GATACGGAGG AGGGGGCAGC AGCTCCCGTC 
    TCCCAGGAGG GGGAACCCAG CTTAGCAGAA ATAAAAGCCC CGATTCAGCA TTCAGATGAA
    CCAGGCATTT TCCATGACAG TCCAGGAGGT ATCCACTTGA ATGAACTGTT GTTTGACCCG
    AGTCAGAACT CCATCACAGG GGAACACAGC CAGCTGTTAG ATGATGGACA TAAGAAAGCG
    CGAAATGCTT ATCTTAACAA TTCCAATTAT GAAGAAGGAG ATGAATATTT TGATAAGAAT
    TTGGCACTCT TTGAGGAAGA AATGGACACC AGACCAAAGG TGTCTTCTCT CCTTAGCCGC
    TTGGCCAATT ATACTAATCT GACACAAGGA GCAAAGGAAC ATGAAGAGGC AGAGAACATC
    ACTGAAGGGA AAAAGAAGCC CACCAAGACT CCCCAAATGG GTACCTTCAT GGGTGTCTAC
    CTCCCATGTC TACAAAATAT TTTTGGAGTG ATCCTGTTTC TGCGCCTCAC ATGGGTGGTG
    GGCACAGCTG GAGTTCTGCA GGCCTTTGCA ATTGTCCTTA TCTGCTGCTG CTGTACGATG
    TTGACTGCTA TCTCCATGAG TGCCATTGCC ACTAATGGAG TGGTGCCAGC TGGAGGCTCA
    TACTTCATGA TTTCCCGAGC ACTGGGCCCA GAGTTTGGTG GAGCTGTTGG CCTCTGCTTC
    TATCTTGGTA CCACATTTGC AGCAGCTATG TATATCCTTG GTGCCATTGA AATATTTCTG
    GTATATATCG TCCCTCGAGC TGCCATCTTT CAGAGTGACG ATGCACTCAA GGAGTCAGCA
    GCCATGCTAA ATAACATGCG TGTCTATGGG ACAGCCTTCT TGGTCCTCAT GGTATTGGTG
    GTATTCATCG GCGTACGCTA TGTGAACAAG TTTGCCTCAC TCTTTCTGGC CTGTGTCATT
    GTGTCCATCT TGGCCATCTA CGCTGGAGCC ATCAAGTCTT CTTTCGCACC TCCACACTTC
    CCGGTCTGCA TGCTGGGCAA CCGCACCCTT TCATCAAGAC ATATTGACGT TTGCTCAAAG
    ACCAAGGAAG TTAACAACAT GACAGTGCCG TCAAAGCTGT GGGGCTTCTT CTGTAACTCA
    AGTCAGTTTT TCAACGCCAC TTGCGATGAG TACTTTGTTC ACAATAATGT CACTTCGATC
    CAGGGCATCC CTGGATTGGC TAGCGGTGTC ATTACAGAGA ATCTTTGGAG TAATTATCTA
    CCAAAGGGAG AGATCATTGA GAAGCCCTCA GCCAAATCTT CCGAAGTCTT AGGCAGCTTA
    AACCATGAGT ATGTCCTTGT GGACATCACC ACCTCTTTCA CTCTTCTGGT GGGCATCTTC
    TTTCCCTCTG TCACAGGCAT CATGGCAGGA TCAAACAGAT CTGGGGATCT GAAAGATGCT
    CAAAAGTCCA TTCCCATTGG CACTATCCTC GCCATCCTGA CCACCTCCTT TGTCTATTTA
    AGCAACGTTG TCCTTTTTGG TGCATGTATT GAAGGTGTTG TCCTCAGAGA CAAGTTTGGA
    GATGCTGTGA AAGGTAATCT GGTGGTAGGA ACCTTATCTT GGCCATCCCC GTGGGTGATT
    GTTATTGGCT CCTTCTTCTC CACATGTGGG GCTGGACTTC AGAGTCTCAC AGGAGCACCG
    AGGCTGCTAC AGGCTATAGC CAAGGATAAC ATTATACCCT TTCTGAGGGT TTTTGGTCAC
    AGCAAAGCCA ATGGGGAACC TACCTGGGCT TTACTTCTGA CTGCTGCCAT TGCAGAACTG
    GGAATCCTTA TTGCCTCCCT GGATCTCGTG GCTCCAATTC TTTCCATGTT TTTTCTCATG
    TGTTACCTCT TTGTGAATTT GGCATGTGCC TTGCAAACAT TGCTTCGAAC ACCCAACTGG
    AGACCCCGGT TCCGCTACTA CCACTGGGCA CTCTCTTTCA TGGGAATGAG TATCTGTCTG
    GCTCTGATGT TCATTTCTTC CTGGTATTAT GCCATCGTAG CTATGGTAAT AGCTGGTATG
    ATCTACAAAT ACATCGAGTA CCAAGGAGCT GAGAAAGAAT GGGGTGATGG TATCCGCGGG
    CTGTCCCTCA GTGCAGCCCG ATTTGCTTTG CTTAGGCTGG AGGAAGGACC ACCACACACT
    AAAAACTGGA GGCCTCAGTT GCTTGTATTG CTGAAACTAG ATGAAGACCT ACACGTCAAG
    CATCCTCGTC TCCTCACCTT TGCTTCACAG CTTAAGGCAG GAAAGGGTCT CACAATTGTG
    GGCTCTGTCA TCGTCGGGAA CTTCCTGGAG AACTATGGTG AAGCGTTAGC TGCTGAGCAG
    ACCATAAAGC ATTTAATGGA GGCAGAGAAG GTGAAAGGGT TCTCACAGCT GGTGGTGGCC
    GCCAAACTGA GGGAGGGCAT TTCCCACCTC ATCCAGTCGT GTGGCCTTGG GGGCATGAAG
    CACAACACAG TGGTGATGGG ATGGCCAAAT GGCTGGCGCC AGAGTGAAGA TGCTCGAGCT
    TGGAAGACTT TTATTGGCAC AGTTCGAGTG ACAACTGCTG CCCATCTGGC CCTGCTGGTG
    GCCAAAAACA TCTCCTTCTT TCCGAGCAAT GTGGAGCAGT TTTCTGAGGG CAACATTGAT
    GTGTGGTGGA TTGTACATGA TGGGGGGATG CTCATGCTAT TACCATTCCT ACTGAAACAG
    CATAAGGTGT GGCGAAAATG CAGCATACGG ATCTTCACAG TAGCCCAGCT AGAAGACAAC
    AGTATTCAGA TGAAGAAGGA CCTGGCCACC TTCTTGTATC ACCTTCGCAT TGAGGCAGAG
    GTGGAAGTGG TAGAGATGCA TGACAGTGAC ATATCCGCAT ACACTTACGA GCGCACCCTG
    ATGATGGAGC AGAGGTCACA GATGCTGCGG CACATGCGCC TGTCCAAGAC GGAGCGAGAC
    CGAGAGGCAC AGCTGGTGAA AGATCGGAAC TCAATGCTAC GACTGACCAG CATCGGCTCT
    GACGAGGATG ACGAGACAGA GACTTATCAG GAGAAGGTGC ACATGACGTG GACGAAAGAC
    AAGTATATGG CGTCTCGGGG ACAAAAGGCC AAGTCAATGG AAGGGTTCCA GGACCTGCTT
    AACATGCGTC CGGACCAGTC CAATGTGAGG CGGATGCATA CAGCAGTGAA GCTCAATGAG
    GTTATAGTTA ACAAGTCCCA TGAAGCAAAG CTGGTTTTGT TGAATATGCC AGGGCCACCC
    CGAAACCCTG AGGGTGACGA AAACTACATG GAGTTCCTGG AAGTACTCAC TGAAGGACTA
    GAGCGAGTCC TTCTTGTCCG AGGTGGTGGC AGTGAAGTGA TCACCATTTA TTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328572.1
    CDS163..3519
    Translation

    Target ORF information:

    RefSeq Version XM_015473086.1
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473086.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACACT TCACAGTGAC TAAAGTAGAG GATACGGAGG AGGGGGCAGC AGCTCCCGTC 
    TCCCAGGAGG GGGAACCCAG CTTAGCAGAA ATAAAAGCCC CGATTCAGCA TTCAGATGAA
    CCAGGCATTT TCCATGACAG TCCAGGAGGT ATCCACTTGA ATGAACTGTT GTTTGACCCG
    AGTCAGAACT CCATCACAGG GGAACACAGC CAGCTGTTAG ATGATGGACA TAAGAAAGCG
    CGAAATGCTT ATCTTAACAA TTCCAATTAT GAAGAAGGAG ATGAATATTT TGATAAGAAT
    TTGGCACTCT TTGAGGAAGA AATGGACACC AGACCAAAGG TGTCTTCTCT CCTTAGCCGC
    TTGGCCAATT ATACTAATCT GACACAAGGA GCAAAGGAAC ATGAAGAGGC AGAGAACATC
    ACTGAAGGGA AAAAGAAGCC CACCAAGACT CCCCAAATGG GTACCTTCAT GGGTGTCTAC
    CTCCCATGTC TACAAAATAT TTTTGGAGTG ATCCTGTTTC TGCGCCTCAC ATGGGTGGTG
    GGCACAGCTG GAGTTCTGCA GGCCTTTGCA ATTGTCCTTA TCTGCTGCTG CTGTACGATG
    TTGACTGCTA TCTCCATGAG TGCCATTGCC ACTAATGGAG TGGTGCCAGC TGGAGGCTCA
    TACTTCATGA TTTCCCGAGC ACTGGGCCCA GAGTTTGGTG GAGCTGTTGG CCTCTGCTTC
    TATCTTGGTA CCACATTTGC AGCAGCTATG TATATCCTTG GTGCCATTGA AATATTTCTG
    GTATATATCG TCCCTCGAGC TGCCATCTTT CAGAGTGACG ATGCACTCAA GGAGTCAGCA
    GCCATGCTAA ATAACATGCG TGTCTATGGG ACAGCCTTCT TGGTCCTCAT GGTATTGGTG
    GTATTCATCG GCGTACGCTA TGTGAACAAG TTTGCCTCAC TCTTTCTGGC CTGTGTCATT
    GTGTCCATCT TGGCCATCTA CGCTGGAGCC ATCAAGTCTT CTTTCGCACC TCCACACTTC
    CCGGTCTGCA TGCTGGGCAA CCGCACCCTT TCATCAAGAC ATATTGACGT TTGCTCAAAG
    ACCAAGGAAG TTAACAACAT GACAGTGCCG TCAAAGCTGT GGGGCTTCTT CTGTAACTCA
    AGTCAGTTTT TCAACGCCAC TTGCGATGAG TACTTTGTTC ACAATAATGT CACTTCGATC
    CAGGGCATCC CTGGATTGGC TAGCGGTGTC ATTACAGAGA ATCTTTGGAG TAATTATCTA
    CCAAAGGGAG AGATCATTGA GAAGCCCTCA GCCAAATCTT CCGAAGTCTT AGGCAGCTTA
    AACCATGAGT ATGTCCTTGT GGACATCACC ACCTCTTTCA CTCTTCTGGT GGGCATCTTC
    TTTCCCTCTG TCACAGGCAT CATGGCAGGA TCAAACAGAT CTGGGGATCT GAAAGATGCT
    CAAAAGTCCA TTCCCATTGG CACTATCCTC GCCATCCTGA CCACCTCCTT TGTCTATTTA
    AGCAACGTTG TCCTTTTTGG TGCATGTATT GAAGGTGTTG TCCTCAGAGA CAAGTTTGGA
    GATGCTGTGA AAGGTAATCT GGTGGTAGGA ACCTTATCTT GGCCATCCCC GTGGGTGATT
    GTTATTGGCT CCTTCTTCTC CACATGTGGG GCTGGACTTC AGAGTCTCAC AGGAGCACCG
    AGGCTGCTAC AGGCTATAGC CAAGGATAAC ATTATACCCT TTCTGAGGGT TTTTGGTCAC
    AGCAAAGCCA ATGGGGAACC TACCTGGGCT TTACTTCTGA CTGCTGCCAT TGCAGAACTG
    GGAATCCTTA TTGCCTCCCT GGATCTCGTG GCTCCAATTC TTTCCATGTT TTTTCTCATG
    TGTTACCTCT TTGTGAATTT GGCATGTGCC TTGCAAACAT TGCTTCGAAC ACCCAACTGG
    AGACCCCGGT TCCGCTACTA CCACTGGGCA CTCTCTTTCA TGGGAATGAG TATCTGTCTG
    GCTCTGATGT TCATTTCTTC CTGGTATTAT GCCATCGTAG CTATGGTAAT AGCTGGTATG
    ATCTACAAAT ACATCGAGTA CCAAGGAGCT GAGAAAGAAT GGGGTGATGG TATCCGCGGG
    CTGTCCCTCA GTGCAGCCCG ATTTGCTTTG CTTAGGCTGG AGGAAGGACC ACCACACACT
    AAAAACTGGA GGCCTCAGTT GCTTGTATTG CTGAAACTAG ATGAAGACCT ACACGTCAAG
    CATCCTCGTC TCCTCACCTT TGCTTCACAG CTTAAGGCAG GAAAGGGTCT CACAATTGTG
    GGCTCTGTCA TCGTCGGGAA CTTCCTGGAG AACTATGGTG AAGCGTTAGC TGCTGAGCAG
    ACCATAAAGC ATTTAATGGA GGCAGAGAAG GTGAAAGGGT TCTCACAGCT GGTGGTGGCC
    GCCAAACTGA GGGAGGGCAT TTCCCACCTC ATCCAGTCGT GTGGCCTTGG GGGCATGAAG
    CACAACACAG TGGTGATGGG ATGGCCAAAT GGCTGGCGCC AGAGTGAAGA TGCTCGAGCT
    TGGAAGACTT TTATTGGCAC AGTTCGAGTG ACAACTGCTG CCCATCTGGC CCTGCTGGTG
    GCCAAAAACA TCTCCTTCTT TCCGAGCAAT GTGGAGCAGT TTTCTGAGGG CAACATTGAT
    GTGTGGTGGA TTGTACATGA TGGGGGGATG CTCATGCTAT TACCATTCCT ACTGAAACAG
    CATAAGGTGT GGCGAAAATG CAGCATACGG ATCTTCACAG TAGCCCAGCT AGAAGACAAC
    AGTATTCAGA TGAAGAAGGA CCTGGCCACC TTCTTGTATC ACCTTCGCAT TGAGGCAGAG
    GTGGAAGTGG TAGAGATGCA TGACAGTGAC ATATCCGCAT ACACTTACGA GCGCACCCTG
    ATGATGGAGC AGAGGTCACA GATGCTGCGG CACATGCGCC TGTCCAAGAC GGAGCGAGAC
    CGAGAGGCAC AGCTGGTGAA AGATCGGAAC TCAATGCTAC GACTGACCAG CATCGGCTCT
    GACGAGGATG ACGAGACAGA GACTTATCAG GAGAAGGTGC ACATGACGTG GACGAAAGAC
    AAGTATATGG CGTCTCGGGG ACAAAAGGCC AAGTCAATGG AAGGGTTCCA GGACCTGCTT
    AACATGCGTC CGGACCAGTC CAATGTGAGG CGGATGCATA CAGCAGTGAA GCTCAATGAG
    GTTATAGTTA ACAAGTCCCA TGAAGCAAAG CTGGTTTTGT TGAATATGCC AGGGCCACCC
    CGAAACCCTG AGGGTGACGA AAACTACATG GAGTTCCTGG AAGTACTCAC TGAAGGACTA
    GAGCGAGTCC TTCTTGTCCG AGGTGGTGGC AGTGAAGTGA TCACCATTTA TTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91642
    Clone ID Related Accession (Same CDS sequence) XM_015473087.2 , XM_015473087.1 , XM_024997562.1
    Accession Version XM_015473087.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACACCA GACCAAAGGT GTCTTCTCTC CTTAGCCGCT TGGCCAATTA TACTAATCTG 
    ACACAAGGAG CAAAGGAACA TGAAGAGGCA GAGAACATCA CTGAAGGGAA AAAGAAGCCC
    ACCAAGACTC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
    TTTGGAGTGA TCCTGTTTCT GCGCCTCACA TGGGTGGTGG GCACAGCTGG AGTTCTGCAG
    GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACGATGT TGACTGCTAT CTCCATGAGT
    GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGAGGCTCAT ACTTCATGAT TTCCCGAGCA
    CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC CTCTGCTTCT ATCTTGGTAC CACATTTGCA
    GCAGCTATGT ATATCCTTGG TGCCATTGAA ATATTTCTGG TATATATCGT CCCTCGAGCT
    GCCATCTTTC AGAGTGACGA TGCACTCAAG GAGTCAGCAG CCATGCTAAA TAACATGCGT
    GTCTATGGGA CAGCCTTCTT GGTCCTCATG GTATTGGTGG TATTCATCGG CGTACGCTAT
    GTGAACAAGT TTGCCTCACT CTTTCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAC
    GCTGGAGCCA TCAAGTCTTC TTTCGCACCT CCACACTTCC CGGTCTGCAT GCTGGGCAAC
    CGCACCCTTT CATCAAGACA TATTGACGTT TGCTCAAAGA CCAAGGAAGT TAACAACATG
    ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC TGTAACTCAA GTCAGTTTTT CAACGCCACT
    TGCGATGAGT ACTTTGTTCA CAATAATGTC ACTTCGATCC AGGGCATCCC TGGATTGGCT
    AGCGGTGTCA TTACAGAGAA TCTTTGGAGT AATTATCTAC CAAAGGGAGA GATCATTGAG
    AAGCCCTCAG CCAAATCTTC CGAAGTCTTA GGCAGCTTAA ACCATGAGTA TGTCCTTGTG
    GACATCACCA CCTCTTTCAC TCTTCTGGTG GGCATCTTCT TTCCCTCTGT CACAGGCATC
    ATGGCAGGAT CAAACAGATC TGGGGATCTG AAAGATGCTC AAAAGTCCAT TCCCATTGGC
    ACTATCCTCG CCATCCTGAC CACCTCCTTT GTCTATTTAA GCAACGTTGT CCTTTTTGGT
    GCATGTATTG AAGGTGTTGT CCTCAGAGAC AAGTTTGGAG ATGCTGTGAA AGGTAATCTG
    GTGGTAGGAA CCTTATCTTG GCCATCCCCG TGGGTGATTG TTATTGGCTC CTTCTTCTCC
    ACATGTGGGG CTGGACTTCA GAGTCTCACA GGAGCACCGA GGCTGCTACA GGCTATAGCC
    AAGGATAACA TTATACCCTT TCTGAGGGTT TTTGGTCACA GCAAAGCCAA TGGGGAACCT
    ACCTGGGCTT TACTTCTGAC TGCTGCCATT GCAGAACTGG GAATCCTTAT TGCCTCCCTG
    GATCTCGTGG CTCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTGAATTTG
    GCATGTGCCT TGCAAACATT GCTTCGAACA CCCAACTGGA GACCCCGGTT CCGCTACTAC
    CACTGGGCAC TCTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
    TGGTATTATG CCATCGTAGC TATGGTAATA GCTGGTATGA TCTACAAATA CATCGAGTAC
    CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA
    TTTGCTTTGC TTAGGCTGGA GGAAGGACCA CCACACACTA AAAACTGGAG GCCTCAGTTG
    CTTGTATTGC TGAAACTAGA TGAAGACCTA CACGTCAAGC ATCCTCGTCT CCTCACCTTT
    GCTTCACAGC TTAAGGCAGG AAAGGGTCTC ACAATTGTGG GCTCTGTCAT CGTCGGGAAC
    TTCCTGGAGA ACTATGGTGA AGCGTTAGCT GCTGAGCAGA CCATAAAGCA TTTAATGGAG
    GCAGAGAAGG TGAAAGGGTT CTCACAGCTG GTGGTGGCCG CCAAACTGAG GGAGGGCATT
    TCCCACCTCA TCCAGTCGTG TGGCCTTGGG GGCATGAAGC ACAACACAGT GGTGATGGGA
    TGGCCAAATG GCTGGCGCCA GAGTGAAGAT GCTCGAGCTT GGAAGACTTT TATTGGCACA
    GTTCGAGTGA CAACTGCTGC CCATCTGGCC CTGCTGGTGG CCAAAAACAT CTCCTTCTTT
    CCGAGCAATG TGGAGCAGTT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTACATGAT
    GGGGGGATGC TCATGCTATT ACCATTCCTA CTGAAACAGC ATAAGGTGTG GCGAAAATGC
    AGCATACGGA TCTTCACAGT AGCCCAGCTA GAAGACAACA GTATTCAGAT GAAGAAGGAC
    CTGGCCACCT TCTTGTATCA CCTTCGCATT GAGGCAGAGG TGGAAGTGGT AGAGATGCAT
    GACAGTGACA TATCCGCATA CACTTACGAG CGCACCCTGA TGATGGAGCA GAGGTCACAG
    ATGCTGCGGC ACATGCGCCT GTCCAAGACG GAGCGAGACC GAGAGGCACA GCTGGTGAAA
    GATCGGAACT CAATGCTACG ACTGACCAGC ATCGGCTCTG ACGAGGATGA CGAGACAGAG
    ACTTATCAGG AGAAGGTGCA CATGACGTGG ACGAAAGACA AGTATATGGC GTCTCGGGGA
    CAAAAGGCCA AGTCAATGGA AGGGTTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
    AATGTGAGGC GGATGCATAC AGCAGTGAAG CTCAATGAGG TTATAGTTAA CAAGTCCCAT
    GAAGCAAAGC TGGTTTTGTT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGACGAA
    AACTACATGG AGTTCCTGGA AGTACTCACT GAAGGACTAG AGCGAGTCCT TCTTGTCCGA
    GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328573.1
    CDS125..3160
    Translation

    Target ORF information:

    RefSeq Version XM_015473087.2
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 member 6 (SLC12A6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473087.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACACCA GACCAAAGGT GTCTTCTCTC CTTAGCCGCT TGGCCAATTA TACTAATCTG 
    ACACAAGGAG CAAAGGAACA TGAAGAGGCA GAGAACATCA CTGAAGGGAA AAAGAAGCCC
    ACCAAGACTC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
    TTTGGAGTGA TCCTGTTTCT GCGCCTCACA TGGGTGGTGG GCACAGCTGG AGTTCTGCAG
    GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACGATGT TGACTGCTAT CTCCATGAGT
    GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGAGGCTCAT ACTTCATGAT TTCCCGAGCA
    CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC CTCTGCTTCT ATCTTGGTAC CACATTTGCA
    GCAGCTATGT ATATCCTTGG TGCCATTGAA ATATTTCTGG TATATATCGT CCCTCGAGCT
    GCCATCTTTC AGAGTGACGA TGCACTCAAG GAGTCAGCAG CCATGCTAAA TAACATGCGT
    GTCTATGGGA CAGCCTTCTT GGTCCTCATG GTATTGGTGG TATTCATCGG CGTACGCTAT
    GTGAACAAGT TTGCCTCACT CTTTCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAC
    GCTGGAGCCA TCAAGTCTTC TTTCGCACCT CCACACTTCC CGGTCTGCAT GCTGGGCAAC
    CGCACCCTTT CATCAAGACA TATTGACGTT TGCTCAAAGA CCAAGGAAGT TAACAACATG
    ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC TGTAACTCAA GTCAGTTTTT CAACGCCACT
    TGCGATGAGT ACTTTGTTCA CAATAATGTC ACTTCGATCC AGGGCATCCC TGGATTGGCT
    AGCGGTGTCA TTACAGAGAA TCTTTGGAGT AATTATCTAC CAAAGGGAGA GATCATTGAG
    AAGCCCTCAG CCAAATCTTC CGAAGTCTTA GGCAGCTTAA ACCATGAGTA TGTCCTTGTG
    GACATCACCA CCTCTTTCAC TCTTCTGGTG GGCATCTTCT TTCCCTCTGT CACAGGCATC
    ATGGCAGGAT CAAACAGATC TGGGGATCTG AAAGATGCTC AAAAGTCCAT TCCCATTGGC
    ACTATCCTCG CCATCCTGAC CACCTCCTTT GTCTATTTAA GCAACGTTGT CCTTTTTGGT
    GCATGTATTG AAGGTGTTGT CCTCAGAGAC AAGTTTGGAG ATGCTGTGAA AGGTAATCTG
    GTGGTAGGAA CCTTATCTTG GCCATCCCCG TGGGTGATTG TTATTGGCTC CTTCTTCTCC
    ACATGTGGGG CTGGACTTCA GAGTCTCACA GGAGCACCGA GGCTGCTACA GGCTATAGCC
    AAGGATAACA TTATACCCTT TCTGAGGGTT TTTGGTCACA GCAAAGCCAA TGGGGAACCT
    ACCTGGGCTT TACTTCTGAC TGCTGCCATT GCAGAACTGG GAATCCTTAT TGCCTCCCTG
    GATCTCGTGG CTCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTGAATTTG
    GCATGTGCCT TGCAAACATT GCTTCGAACA CCCAACTGGA GACCCCGGTT CCGCTACTAC
    CACTGGGCAC TCTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
    TGGTATTATG CCATCGTAGC TATGGTAATA GCTGGTATGA TCTACAAATA CATCGAGTAC
    CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA
    TTTGCTTTGC TTAGGCTGGA GGAAGGACCA CCACACACTA AAAACTGGAG GCCTCAGTTG
    CTTGTATTGC TGAAACTAGA TGAAGACCTA CACGTCAAGC ATCCTCGTCT CCTCACCTTT
    GCTTCACAGC TTAAGGCAGG AAAGGGTCTC ACAATTGTGG GCTCTGTCAT CGTCGGGAAC
    TTCCTGGAGA ACTATGGTGA AGCGTTAGCT GCTGAGCAGA CCATAAAGCA TTTAATGGAG
    GCAGAGAAGG TGAAAGGGTT CTCACAGCTG GTGGTGGCCG CCAAACTGAG GGAGGGCATT
    TCCCACCTCA TCCAGTCGTG TGGCCTTGGG GGCATGAAGC ACAACACAGT GGTGATGGGA
    TGGCCAAATG GCTGGCGCCA GAGTGAAGAT GCTCGAGCTT GGAAGACTTT TATTGGCACA
    GTTCGAGTGA CAACTGCTGC CCATCTGGCC CTGCTGGTGG CCAAAAACAT CTCCTTCTTT
    CCGAGCAATG TGGAGCAGTT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTACATGAT
    GGGGGGATGC TCATGCTATT ACCATTCCTA CTGAAACAGC ATAAGGTGTG GCGAAAATGC
    AGCATACGGA TCTTCACAGT AGCCCAGCTA GAAGACAACA GTATTCAGAT GAAGAAGGAC
    CTGGCCACCT TCTTGTATCA CCTTCGCATT GAGGCAGAGG TGGAAGTGGT AGAGATGCAT
    GACAGTGACA TATCCGCATA CACTTACGAG CGCACCCTGA TGATGGAGCA GAGGTCACAG
    ATGCTGCGGC ACATGCGCCT GTCCAAGACG GAGCGAGACC GAGAGGCACA GCTGGTGAAA
    GATCGGAACT CAATGCTACG ACTGACCAGC ATCGGCTCTG ACGAGGATGA CGAGACAGAG
    ACTTATCAGG AGAAGGTGCA CATGACGTGG ACGAAAGACA AGTATATGGC GTCTCGGGGA
    CAAAAGGCCA AGTCAATGGA AGGGTTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
    AATGTGAGGC GGATGCATAC AGCAGTGAAG CTCAATGAGG TTATAGTTAA CAAGTCCCAT
    GAAGCAAAGC TGGTTTTGTT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGACGAA
    AACTACATGG AGTTCCTGGA AGTACTCACT GAAGGACTAG AGCGAGTCCT TCTTGTCCGA
    GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91642
    Clone ID Related Accession (Same CDS sequence) XM_015473087.2 , XM_015473087.1 , XM_024997562.1
    Accession Version XM_015473087.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACACCA GACCAAAGGT GTCTTCTCTC CTTAGCCGCT TGGCCAATTA TACTAATCTG 
    ACACAAGGAG CAAAGGAACA TGAAGAGGCA GAGAACATCA CTGAAGGGAA AAAGAAGCCC
    ACCAAGACTC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
    TTTGGAGTGA TCCTGTTTCT GCGCCTCACA TGGGTGGTGG GCACAGCTGG AGTTCTGCAG
    GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACGATGT TGACTGCTAT CTCCATGAGT
    GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGAGGCTCAT ACTTCATGAT TTCCCGAGCA
    CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC CTCTGCTTCT ATCTTGGTAC CACATTTGCA
    GCAGCTATGT ATATCCTTGG TGCCATTGAA ATATTTCTGG TATATATCGT CCCTCGAGCT
    GCCATCTTTC AGAGTGACGA TGCACTCAAG GAGTCAGCAG CCATGCTAAA TAACATGCGT
    GTCTATGGGA CAGCCTTCTT GGTCCTCATG GTATTGGTGG TATTCATCGG CGTACGCTAT
    GTGAACAAGT TTGCCTCACT CTTTCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAC
    GCTGGAGCCA TCAAGTCTTC TTTCGCACCT CCACACTTCC CGGTCTGCAT GCTGGGCAAC
    CGCACCCTTT CATCAAGACA TATTGACGTT TGCTCAAAGA CCAAGGAAGT TAACAACATG
    ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC TGTAACTCAA GTCAGTTTTT CAACGCCACT
    TGCGATGAGT ACTTTGTTCA CAATAATGTC ACTTCGATCC AGGGCATCCC TGGATTGGCT
    AGCGGTGTCA TTACAGAGAA TCTTTGGAGT AATTATCTAC CAAAGGGAGA GATCATTGAG
    AAGCCCTCAG CCAAATCTTC CGAAGTCTTA GGCAGCTTAA ACCATGAGTA TGTCCTTGTG
    GACATCACCA CCTCTTTCAC TCTTCTGGTG GGCATCTTCT TTCCCTCTGT CACAGGCATC
    ATGGCAGGAT CAAACAGATC TGGGGATCTG AAAGATGCTC AAAAGTCCAT TCCCATTGGC
    ACTATCCTCG CCATCCTGAC CACCTCCTTT GTCTATTTAA GCAACGTTGT CCTTTTTGGT
    GCATGTATTG AAGGTGTTGT CCTCAGAGAC AAGTTTGGAG ATGCTGTGAA AGGTAATCTG
    GTGGTAGGAA CCTTATCTTG GCCATCCCCG TGGGTGATTG TTATTGGCTC CTTCTTCTCC
    ACATGTGGGG CTGGACTTCA GAGTCTCACA GGAGCACCGA GGCTGCTACA GGCTATAGCC
    AAGGATAACA TTATACCCTT TCTGAGGGTT TTTGGTCACA GCAAAGCCAA TGGGGAACCT
    ACCTGGGCTT TACTTCTGAC TGCTGCCATT GCAGAACTGG GAATCCTTAT TGCCTCCCTG
    GATCTCGTGG CTCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTGAATTTG
    GCATGTGCCT TGCAAACATT GCTTCGAACA CCCAACTGGA GACCCCGGTT CCGCTACTAC
    CACTGGGCAC TCTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
    TGGTATTATG CCATCGTAGC TATGGTAATA GCTGGTATGA TCTACAAATA CATCGAGTAC
    CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA
    TTTGCTTTGC TTAGGCTGGA GGAAGGACCA CCACACACTA AAAACTGGAG GCCTCAGTTG
    CTTGTATTGC TGAAACTAGA TGAAGACCTA CACGTCAAGC ATCCTCGTCT CCTCACCTTT
    GCTTCACAGC TTAAGGCAGG AAAGGGTCTC ACAATTGTGG GCTCTGTCAT CGTCGGGAAC
    TTCCTGGAGA ACTATGGTGA AGCGTTAGCT GCTGAGCAGA CCATAAAGCA TTTAATGGAG
    GCAGAGAAGG TGAAAGGGTT CTCACAGCTG GTGGTGGCCG CCAAACTGAG GGAGGGCATT
    TCCCACCTCA TCCAGTCGTG TGGCCTTGGG GGCATGAAGC ACAACACAGT GGTGATGGGA
    TGGCCAAATG GCTGGCGCCA GAGTGAAGAT GCTCGAGCTT GGAAGACTTT TATTGGCACA
    GTTCGAGTGA CAACTGCTGC CCATCTGGCC CTGCTGGTGG CCAAAAACAT CTCCTTCTTT
    CCGAGCAATG TGGAGCAGTT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTACATGAT
    GGGGGGATGC TCATGCTATT ACCATTCCTA CTGAAACAGC ATAAGGTGTG GCGAAAATGC
    AGCATACGGA TCTTCACAGT AGCCCAGCTA GAAGACAACA GTATTCAGAT GAAGAAGGAC
    CTGGCCACCT TCTTGTATCA CCTTCGCATT GAGGCAGAGG TGGAAGTGGT AGAGATGCAT
    GACAGTGACA TATCCGCATA CACTTACGAG CGCACCCTGA TGATGGAGCA GAGGTCACAG
    ATGCTGCGGC ACATGCGCCT GTCCAAGACG GAGCGAGACC GAGAGGCACA GCTGGTGAAA
    GATCGGAACT CAATGCTACG ACTGACCAGC ATCGGCTCTG ACGAGGATGA CGAGACAGAG
    ACTTATCAGG AGAAGGTGCA CATGACGTGG ACGAAAGACA AGTATATGGC GTCTCGGGGA
    CAAAAGGCCA AGTCAATGGA AGGGTTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
    AATGTGAGGC GGATGCATAC AGCAGTGAAG CTCAATGAGG TTATAGTTAA CAAGTCCCAT
    GAAGCAAAGC TGGTTTTGTT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGACGAA
    AACTACATGG AGTTCCTGGA AGTACTCACT GAAGGACTAG AGCGAGTCCT TCTTGTCCGA
    GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328573.1
    CDS223..3258
    Translation

    Target ORF information:

    RefSeq Version XM_015473087.1
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473087.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACACCA GACCAAAGGT GTCTTCTCTC CTTAGCCGCT TGGCCAATTA TACTAATCTG 
    ACACAAGGAG CAAAGGAACA TGAAGAGGCA GAGAACATCA CTGAAGGGAA AAAGAAGCCC
    ACCAAGACTC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
    TTTGGAGTGA TCCTGTTTCT GCGCCTCACA TGGGTGGTGG GCACAGCTGG AGTTCTGCAG
    GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACGATGT TGACTGCTAT CTCCATGAGT
    GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGAGGCTCAT ACTTCATGAT TTCCCGAGCA
    CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC CTCTGCTTCT ATCTTGGTAC CACATTTGCA
    GCAGCTATGT ATATCCTTGG TGCCATTGAA ATATTTCTGG TATATATCGT CCCTCGAGCT
    GCCATCTTTC AGAGTGACGA TGCACTCAAG GAGTCAGCAG CCATGCTAAA TAACATGCGT
    GTCTATGGGA CAGCCTTCTT GGTCCTCATG GTATTGGTGG TATTCATCGG CGTACGCTAT
    GTGAACAAGT TTGCCTCACT CTTTCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAC
    GCTGGAGCCA TCAAGTCTTC TTTCGCACCT CCACACTTCC CGGTCTGCAT GCTGGGCAAC
    CGCACCCTTT CATCAAGACA TATTGACGTT TGCTCAAAGA CCAAGGAAGT TAACAACATG
    ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC TGTAACTCAA GTCAGTTTTT CAACGCCACT
    TGCGATGAGT ACTTTGTTCA CAATAATGTC ACTTCGATCC AGGGCATCCC TGGATTGGCT
    AGCGGTGTCA TTACAGAGAA TCTTTGGAGT AATTATCTAC CAAAGGGAGA GATCATTGAG
    AAGCCCTCAG CCAAATCTTC CGAAGTCTTA GGCAGCTTAA ACCATGAGTA TGTCCTTGTG
    GACATCACCA CCTCTTTCAC TCTTCTGGTG GGCATCTTCT TTCCCTCTGT CACAGGCATC
    ATGGCAGGAT CAAACAGATC TGGGGATCTG AAAGATGCTC AAAAGTCCAT TCCCATTGGC
    ACTATCCTCG CCATCCTGAC CACCTCCTTT GTCTATTTAA GCAACGTTGT CCTTTTTGGT
    GCATGTATTG AAGGTGTTGT CCTCAGAGAC AAGTTTGGAG ATGCTGTGAA AGGTAATCTG
    GTGGTAGGAA CCTTATCTTG GCCATCCCCG TGGGTGATTG TTATTGGCTC CTTCTTCTCC
    ACATGTGGGG CTGGACTTCA GAGTCTCACA GGAGCACCGA GGCTGCTACA GGCTATAGCC
    AAGGATAACA TTATACCCTT TCTGAGGGTT TTTGGTCACA GCAAAGCCAA TGGGGAACCT
    ACCTGGGCTT TACTTCTGAC TGCTGCCATT GCAGAACTGG GAATCCTTAT TGCCTCCCTG
    GATCTCGTGG CTCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTGAATTTG
    GCATGTGCCT TGCAAACATT GCTTCGAACA CCCAACTGGA GACCCCGGTT CCGCTACTAC
    CACTGGGCAC TCTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
    TGGTATTATG CCATCGTAGC TATGGTAATA GCTGGTATGA TCTACAAATA CATCGAGTAC
    CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA
    TTTGCTTTGC TTAGGCTGGA GGAAGGACCA CCACACACTA AAAACTGGAG GCCTCAGTTG
    CTTGTATTGC TGAAACTAGA TGAAGACCTA CACGTCAAGC ATCCTCGTCT CCTCACCTTT
    GCTTCACAGC TTAAGGCAGG AAAGGGTCTC ACAATTGTGG GCTCTGTCAT CGTCGGGAAC
    TTCCTGGAGA ACTATGGTGA AGCGTTAGCT GCTGAGCAGA CCATAAAGCA TTTAATGGAG
    GCAGAGAAGG TGAAAGGGTT CTCACAGCTG GTGGTGGCCG CCAAACTGAG GGAGGGCATT
    TCCCACCTCA TCCAGTCGTG TGGCCTTGGG GGCATGAAGC ACAACACAGT GGTGATGGGA
    TGGCCAAATG GCTGGCGCCA GAGTGAAGAT GCTCGAGCTT GGAAGACTTT TATTGGCACA
    GTTCGAGTGA CAACTGCTGC CCATCTGGCC CTGCTGGTGG CCAAAAACAT CTCCTTCTTT
    CCGAGCAATG TGGAGCAGTT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTACATGAT
    GGGGGGATGC TCATGCTATT ACCATTCCTA CTGAAACAGC ATAAGGTGTG GCGAAAATGC
    AGCATACGGA TCTTCACAGT AGCCCAGCTA GAAGACAACA GTATTCAGAT GAAGAAGGAC
    CTGGCCACCT TCTTGTATCA CCTTCGCATT GAGGCAGAGG TGGAAGTGGT AGAGATGCAT
    GACAGTGACA TATCCGCATA CACTTACGAG CGCACCCTGA TGATGGAGCA GAGGTCACAG
    ATGCTGCGGC ACATGCGCCT GTCCAAGACG GAGCGAGACC GAGAGGCACA GCTGGTGAAA
    GATCGGAACT CAATGCTACG ACTGACCAGC ATCGGCTCTG ACGAGGATGA CGAGACAGAG
    ACTTATCAGG AGAAGGTGCA CATGACGTGG ACGAAAGACA AGTATATGGC GTCTCGGGGA
    CAAAAGGCCA AGTCAATGGA AGGGTTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
    AATGTGAGGC GGATGCATAC AGCAGTGAAG CTCAATGAGG TTATAGTTAA CAAGTCCCAT
    GAAGCAAAGC TGGTTTTGTT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGACGAA
    AACTACATGG AGTTCCTGGA AGTACTCACT GAAGGACTAG AGCGAGTCCT TCTTGTCCGA
    GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91642
    Clone ID Related Accession (Same CDS sequence) XM_015473087.2 , XM_015473087.1 , XM_024997562.1
    Accession Version XM_024997562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3036bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACACCA GACCAAAGGT GTCTTCTCTC CTTAGCCGCT TGGCCAATTA TACTAATCTG 
    ACACAAGGAG CAAAGGAACA TGAAGAGGCA GAGAACATCA CTGAAGGGAA AAAGAAGCCC
    ACCAAGACTC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
    TTTGGAGTGA TCCTGTTTCT GCGCCTCACA TGGGTGGTGG GCACAGCTGG AGTTCTGCAG
    GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACGATGT TGACTGCTAT CTCCATGAGT
    GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGAGGCTCAT ACTTCATGAT TTCCCGAGCA
    CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC CTCTGCTTCT ATCTTGGTAC CACATTTGCA
    GCAGCTATGT ATATCCTTGG TGCCATTGAA ATATTTCTGG TATATATCGT CCCTCGAGCT
    GCCATCTTTC AGAGTGACGA TGCACTCAAG GAGTCAGCAG CCATGCTAAA TAACATGCGT
    GTCTATGGGA CAGCCTTCTT GGTCCTCATG GTATTGGTGG TATTCATCGG CGTACGCTAT
    GTGAACAAGT TTGCCTCACT CTTTCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAC
    GCTGGAGCCA TCAAGTCTTC TTTCGCACCT CCACACTTCC CGGTCTGCAT GCTGGGCAAC
    CGCACCCTTT CATCAAGACA TATTGACGTT TGCTCAAAGA CCAAGGAAGT TAACAACATG
    ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC TGTAACTCAA GTCAGTTTTT CAACGCCACT
    TGCGATGAGT ACTTTGTTCA CAATAATGTC ACTTCGATCC AGGGCATCCC TGGATTGGCT
    AGCGGTGTCA TTACAGAGAA TCTTTGGAGT AATTATCTAC CAAAGGGAGA GATCATTGAG
    AAGCCCTCAG CCAAATCTTC CGAAGTCTTA GGCAGCTTAA ACCATGAGTA TGTCCTTGTG
    GACATCACCA CCTCTTTCAC TCTTCTGGTG GGCATCTTCT TTCCCTCTGT CACAGGCATC
    ATGGCAGGAT CAAACAGATC TGGGGATCTG AAAGATGCTC AAAAGTCCAT TCCCATTGGC
    ACTATCCTCG CCATCCTGAC CACCTCCTTT GTCTATTTAA GCAACGTTGT CCTTTTTGGT
    GCATGTATTG AAGGTGTTGT CCTCAGAGAC AAGTTTGGAG ATGCTGTGAA AGGTAATCTG
    GTGGTAGGAA CCTTATCTTG GCCATCCCCG TGGGTGATTG TTATTGGCTC CTTCTTCTCC
    ACATGTGGGG CTGGACTTCA GAGTCTCACA GGAGCACCGA GGCTGCTACA GGCTATAGCC
    AAGGATAACA TTATACCCTT TCTGAGGGTT TTTGGTCACA GCAAAGCCAA TGGGGAACCT
    ACCTGGGCTT TACTTCTGAC TGCTGCCATT GCAGAACTGG GAATCCTTAT TGCCTCCCTG
    GATCTCGTGG CTCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTGAATTTG
    GCATGTGCCT TGCAAACATT GCTTCGAACA CCCAACTGGA GACCCCGGTT CCGCTACTAC
    CACTGGGCAC TCTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
    TGGTATTATG CCATCGTAGC TATGGTAATA GCTGGTATGA TCTACAAATA CATCGAGTAC
    CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA
    TTTGCTTTGC TTAGGCTGGA GGAAGGACCA CCACACACTA AAAACTGGAG GCCTCAGTTG
    CTTGTATTGC TGAAACTAGA TGAAGACCTA CACGTCAAGC ATCCTCGTCT CCTCACCTTT
    GCTTCACAGC TTAAGGCAGG AAAGGGTCTC ACAATTGTGG GCTCTGTCAT CGTCGGGAAC
    TTCCTGGAGA ACTATGGTGA AGCGTTAGCT GCTGAGCAGA CCATAAAGCA TTTAATGGAG
    GCAGAGAAGG TGAAAGGGTT CTCACAGCTG GTGGTGGCCG CCAAACTGAG GGAGGGCATT
    TCCCACCTCA TCCAGTCGTG TGGCCTTGGG GGCATGAAGC ACAACACAGT GGTGATGGGA
    TGGCCAAATG GCTGGCGCCA GAGTGAAGAT GCTCGAGCTT GGAAGACTTT TATTGGCACA
    GTTCGAGTGA CAACTGCTGC CCATCTGGCC CTGCTGGTGG CCAAAAACAT CTCCTTCTTT
    CCGAGCAATG TGGAGCAGTT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTACATGAT
    GGGGGGATGC TCATGCTATT ACCATTCCTA CTGAAACAGC ATAAGGTGTG GCGAAAATGC
    AGCATACGGA TCTTCACAGT AGCCCAGCTA GAAGACAACA GTATTCAGAT GAAGAAGGAC
    CTGGCCACCT TCTTGTATCA CCTTCGCATT GAGGCAGAGG TGGAAGTGGT AGAGATGCAT
    GACAGTGACA TATCCGCATA CACTTACGAG CGCACCCTGA TGATGGAGCA GAGGTCACAG
    ATGCTGCGGC ACATGCGCCT GTCCAAGACG GAGCGAGACC GAGAGGCACA GCTGGTGAAA
    GATCGGAACT CAATGCTACG ACTGACCAGC ATCGGCTCTG ACGAGGATGA CGAGACAGAG
    ACTTATCAGG AGAAGGTGCA CATGACGTGG ACGAAAGACA AGTATATGGC GTCTCGGGGA
    CAAAAGGCCA AGTCAATGGA AGGGTTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
    AATGTGAGGC GGATGCATAC AGCAGTGAAG CTCAATGAGG TTATAGTTAA CAAGTCCCAT
    GAAGCAAAGC TGGTTTTGTT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGACGAA
    AACTACATGG AGTTCCTGGA AGTACTCACT GAAGGACTAG AGCGAGTCCT TCTTGTCCGA
    GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853330.1
    CDS124..3159
    Translation

    Target ORF information:

    RefSeq Version XM_024997562.1
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 member 6 (SLC12A6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACACCA GACCAAAGGT GTCTTCTCTC CTTAGCCGCT TGGCCAATTA TACTAATCTG 
    ACACAAGGAG CAAAGGAACA TGAAGAGGCA GAGAACATCA CTGAAGGGAA AAAGAAGCCC
    ACCAAGACTC CCCAAATGGG TACCTTCATG GGTGTCTACC TCCCATGTCT ACAAAATATT
    TTTGGAGTGA TCCTGTTTCT GCGCCTCACA TGGGTGGTGG GCACAGCTGG AGTTCTGCAG
    GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC TGTACGATGT TGACTGCTAT CTCCATGAGT
    GCCATTGCCA CTAATGGAGT GGTGCCAGCT GGAGGCTCAT ACTTCATGAT TTCCCGAGCA
    CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC CTCTGCTTCT ATCTTGGTAC CACATTTGCA
    GCAGCTATGT ATATCCTTGG TGCCATTGAA ATATTTCTGG TATATATCGT CCCTCGAGCT
    GCCATCTTTC AGAGTGACGA TGCACTCAAG GAGTCAGCAG CCATGCTAAA TAACATGCGT
    GTCTATGGGA CAGCCTTCTT GGTCCTCATG GTATTGGTGG TATTCATCGG CGTACGCTAT
    GTGAACAAGT TTGCCTCACT CTTTCTGGCC TGTGTCATTG TGTCCATCTT GGCCATCTAC
    GCTGGAGCCA TCAAGTCTTC TTTCGCACCT CCACACTTCC CGGTCTGCAT GCTGGGCAAC
    CGCACCCTTT CATCAAGACA TATTGACGTT TGCTCAAAGA CCAAGGAAGT TAACAACATG
    ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC TGTAACTCAA GTCAGTTTTT CAACGCCACT
    TGCGATGAGT ACTTTGTTCA CAATAATGTC ACTTCGATCC AGGGCATCCC TGGATTGGCT
    AGCGGTGTCA TTACAGAGAA TCTTTGGAGT AATTATCTAC CAAAGGGAGA GATCATTGAG
    AAGCCCTCAG CCAAATCTTC CGAAGTCTTA GGCAGCTTAA ACCATGAGTA TGTCCTTGTG
    GACATCACCA CCTCTTTCAC TCTTCTGGTG GGCATCTTCT TTCCCTCTGT CACAGGCATC
    ATGGCAGGAT CAAACAGATC TGGGGATCTG AAAGATGCTC AAAAGTCCAT TCCCATTGGC
    ACTATCCTCG CCATCCTGAC CACCTCCTTT GTCTATTTAA GCAACGTTGT CCTTTTTGGT
    GCATGTATTG AAGGTGTTGT CCTCAGAGAC AAGTTTGGAG ATGCTGTGAA AGGTAATCTG
    GTGGTAGGAA CCTTATCTTG GCCATCCCCG TGGGTGATTG TTATTGGCTC CTTCTTCTCC
    ACATGTGGGG CTGGACTTCA GAGTCTCACA GGAGCACCGA GGCTGCTACA GGCTATAGCC
    AAGGATAACA TTATACCCTT TCTGAGGGTT TTTGGTCACA GCAAAGCCAA TGGGGAACCT
    ACCTGGGCTT TACTTCTGAC TGCTGCCATT GCAGAACTGG GAATCCTTAT TGCCTCCCTG
    GATCTCGTGG CTCCAATTCT TTCCATGTTT TTTCTCATGT GTTACCTCTT TGTGAATTTG
    GCATGTGCCT TGCAAACATT GCTTCGAACA CCCAACTGGA GACCCCGGTT CCGCTACTAC
    CACTGGGCAC TCTCTTTCAT GGGAATGAGT ATCTGTCTGG CTCTGATGTT CATTTCTTCC
    TGGTATTATG CCATCGTAGC TATGGTAATA GCTGGTATGA TCTACAAATA CATCGAGTAC
    CAAGGAGCTG AGAAAGAATG GGGTGATGGT ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA
    TTTGCTTTGC TTAGGCTGGA GGAAGGACCA CCACACACTA AAAACTGGAG GCCTCAGTTG
    CTTGTATTGC TGAAACTAGA TGAAGACCTA CACGTCAAGC ATCCTCGTCT CCTCACCTTT
    GCTTCACAGC TTAAGGCAGG AAAGGGTCTC ACAATTGTGG GCTCTGTCAT CGTCGGGAAC
    TTCCTGGAGA ACTATGGTGA AGCGTTAGCT GCTGAGCAGA CCATAAAGCA TTTAATGGAG
    GCAGAGAAGG TGAAAGGGTT CTCACAGCTG GTGGTGGCCG CCAAACTGAG GGAGGGCATT
    TCCCACCTCA TCCAGTCGTG TGGCCTTGGG GGCATGAAGC ACAACACAGT GGTGATGGGA
    TGGCCAAATG GCTGGCGCCA GAGTGAAGAT GCTCGAGCTT GGAAGACTTT TATTGGCACA
    GTTCGAGTGA CAACTGCTGC CCATCTGGCC CTGCTGGTGG CCAAAAACAT CTCCTTCTTT
    CCGAGCAATG TGGAGCAGTT TTCTGAGGGC AACATTGATG TGTGGTGGAT TGTACATGAT
    GGGGGGATGC TCATGCTATT ACCATTCCTA CTGAAACAGC ATAAGGTGTG GCGAAAATGC
    AGCATACGGA TCTTCACAGT AGCCCAGCTA GAAGACAACA GTATTCAGAT GAAGAAGGAC
    CTGGCCACCT TCTTGTATCA CCTTCGCATT GAGGCAGAGG TGGAAGTGGT AGAGATGCAT
    GACAGTGACA TATCCGCATA CACTTACGAG CGCACCCTGA TGATGGAGCA GAGGTCACAG
    ATGCTGCGGC ACATGCGCCT GTCCAAGACG GAGCGAGACC GAGAGGCACA GCTGGTGAAA
    GATCGGAACT CAATGCTACG ACTGACCAGC ATCGGCTCTG ACGAGGATGA CGAGACAGAG
    ACTTATCAGG AGAAGGTGCA CATGACGTGG ACGAAAGACA AGTATATGGC GTCTCGGGGA
    CAAAAGGCCA AGTCAATGGA AGGGTTCCAG GACCTGCTTA ACATGCGTCC GGACCAGTCC
    AATGTGAGGC GGATGCATAC AGCAGTGAAG CTCAATGAGG TTATAGTTAA CAAGTCCCAT
    GAAGCAAAGC TGGTTTTGTT GAATATGCCA GGGCCACCCC GAAACCCTGA GGGTGACGAA
    AACTACATGG AGTTCCTGGA AGTACTCACT GAAGGACTAG AGCGAGTCCT TCTTGTCCGA
    GGTGGTGGCA GTGAAGTGAT CACCATTTAT TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37403
    Clone ID Related Accession (Same CDS sequence) NM_001193116.1
    Accession Version NM_001193116.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3453bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000010.1. On Jul 17, 2010 this sequence version replaced XM_868592.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571260.47539.1, SRR5765710.8394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145425 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
    AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT
    TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG
    AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC
    CGGACATCTA ACCCCCAGGA TGTTACTGAG GACCCGAGTC AGAACTCCAT CACAGGGGAA
    CACAGCCAGC TGTTAGATGA TGGACATAAG AAAGCGCGAA ATGCTTATCT TAACAATTCC
    AATTATGAAG AAGGAGATGA ATATTTTGAT AAGAATTTGG CACTCTTTGA GGAAGAAATG
    GACACCAGAC CAAAGGTGTC TTCTCTCCTT AGCCGCTTGG CCAATTATAC TAATCTGACA
    CAAGGAGCAA AGGAACATGA AGAGGCAGAG AACATCACTG AAGGGAAAAA GAAGCCCACC
    AAGACTCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
    GGAGTGATCC TGTTTCTGCG CCTCACATGG GTGGTGGGCA CAGCTGGAGT TCTGCAGGCC
    TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACGATGTTGA CTGCTATCTC CATGAGTGCC
    ATTGCCACTA ATGGAGTGGT GCCAGCTGGA GGCTCATACT TCATGATTTC CCGAGCACTG
    GGCCCAGAGT TTGGTGGAGC TGTTGGCCTC TGCTTCTATC TTGGTACCAC ATTTGCAGCA
    GCTATGTATA TCCTTGGTGC CATTGAAATA TTTCTGGTAT ATATCGTCCC TCGAGCTGCC
    ATCTTTCAGA GTGACGATGC ACTCAAGGAG TCAGCAGCCA TGCTAAATAA CATGCGTGTC
    TATGGGACAG CCTTCTTGGT CCTCATGGTA TTGGTGGTAT TCATCGGCGT ACGCTATGTG
    AACAAGTTTG CCTCACTCTT TCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTACGCT
    GGAGCCATCA AGTCTTCTTT CGCACCTCCA CACTTCCCGG TCTGCATGCT GGGCAACCGC
    ACCCTTTCAT CAAGACATAT TGACGTTTGC TCAAAGACCA AGGAAGTTAA CAACATGACA
    GTGCCGTCAA AGCTGTGGGG CTTCTTCTGT AACTCAAGTC AGTTTTTCAA CGCCACTTGC
    GATGAGTACT TTGTTCACAA TAATGTCACT TCGATCCAGG GCATCCCTGG ATTGGCTAGC
    GGTGTCATTA CAGAGAATCT TTGGAGTAAT TATCTACCAA AGGGAGAGAT CATTGAGAAG
    CCCTCAGCCA AATCTTCCGA AGTCTTAGGC AGCTTAAACC ATGAGTATGT CCTTGTGGAC
    ATCACCACCT CTTTCACTCT TCTGGTGGGC ATCTTCTTTC CCTCTGTCAC AGGCATCATG
    GCAGGATCAA ACAGATCTGG GGATCTGAAA GATGCTCAAA AGTCCATTCC CATTGGCACT
    ATCCTCGCCA TCCTGACCAC CTCCTTTGTC TATTTAAGCA ACGTTGTCCT TTTTGGTGCA
    TGTATTGAAG GTGTTGTCCT CAGAGACAAG TTTGGAGATG CTGTGAAAGG TAATCTGGTG
    GTAGGAACCT TATCTTGGCC ATCCCCGTGG GTGATTGTTA TTGGCTCCTT CTTCTCCACA
    TGTGGGGCTG GACTTCAGAG TCTCACAGGA GCACCGAGGC TGCTACAGGC TATAGCCAAG
    GATAACATTA TACCCTTTCT GAGGGTTTTT GGTCACAGCA AAGCCAATGG GGAACCTACC
    TGGGCTTTAC TTCTGACTGC TGCCATTGCA GAACTGGGAA TCCTTATTGC CTCCCTGGAT
    CTCGTGGCTC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT GAATTTGGCA
    TGTGCCTTGC AAACATTGCT TCGAACACCC AACTGGAGAC CCCGGTTCCG CTACTACCAC
    TGGGCACTCT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG
    TATTATGCCA TCGTAGCTAT GGTAATAGCT GGTATGATCT ACAAATACAT CGAGTACCAA
    GGAGCTGAGA AAGAATGGGG TGATGGTATC CGCGGGCTGT CCCTCAGTGC AGCCCGATTT
    GCTTTGCTTA GGCTGGAGGA AGGACCACCA CACACTAAAA ACTGGAGGCC TCAGTTGCTT
    GTATTGCTGA AACTAGATGA AGACCTACAC GTCAAGCATC CTCGTCTCCT CACCTTTGCT
    TCACAGCTTA AGGCAGGAAA GGGTCTCACA ATTGTGGGCT CTGTCATCGT CGGGAACTTC
    CTGGAGAACT ATGGTGAAGC GTTAGCTGCT GAGCAGACCA TAAAGCATTT AATGGAGGCA
    GAGAAGGTGA AAGGGTTCTC ACAGCTGGTG GTGGCCGCCA AACTGAGGGA GGGCATTTCC
    CACCTCATCC AGTCGTGTGG CCTTGGGGGC ATGAAGCACA ACACAGTGGT GATGGGATGG
    CCAAATGGCT GGCGCCAGAG TGAAGATGCT CGAGCTTGGA AGACTTTTAT TGGCACAGTT
    CGAGTGACAA CTGCTGCCCA TCTGGCCCTG CTGGTGGCCA AAAACATCTC CTTCTTTCCG
    AGCAATGTGG AGCAGTTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT ACATGATGGG
    GGGATGCTCA TGCTATTACC ATTCCTACTG AAACAGCATA AGGTGTGGCG AAAATGCAGC
    ATACGGATCT TCACAGTAGC CCAGCTAGAA GACAACAGTA TTCAGATGAA GAAGGACCTG
    GCCACCTTCT TGTATCACCT TCGCATTGAG GCAGAGGTGG AAGTGGTAGA GATGCATGAC
    AGTGACATAT CCGCATACAC TTACGAGCGC ACCCTGATGA TGGAGCAGAG GTCACAGATG
    CTGCGGCACA TGCGCCTGTC CAAGACGGAG CGAGACCGAG AGGCACAGCT GGTGAAAGAT
    CGGAACTCAA TGCTACGACT GACCAGCATC GGCTCTGACG AGGATGACGA GACAGAGACT
    TATCAGGAGA AGGTGCACAT GACGTGGACG AAAGACAAGT ATATGGCGTC TCGGGGACAA
    AAGGCCAAGT CAATGGAAGG GTTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT
    GTGAGGCGGA TGCATACAGC AGTGAAGCTC AATGAGGTTA TAGTTAACAA GTCCCATGAA
    GCAAAGCTGG TTTTGTTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGACGAAAAC
    TACATGGAGT TCCTGGAAGT ACTCACTGAA GGACTAGAGC GAGTCCTTCT TGTCCGAGGT
    GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180045.1
    CDS1..3453
    Translation

    Target ORF information:

    RefSeq Version NM_001193116.1
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 member 6 (SLC12A6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193116.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
    AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT
    TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG
    AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC
    CGGACATCTA ACCCCCAGGA TGTTACTGAG GACCCGAGTC AGAACTCCAT CACAGGGGAA
    CACAGCCAGC TGTTAGATGA TGGACATAAG AAAGCGCGAA ATGCTTATCT TAACAATTCC
    AATTATGAAG AAGGAGATGA ATATTTTGAT AAGAATTTGG CACTCTTTGA GGAAGAAATG
    GACACCAGAC CAAAGGTGTC TTCTCTCCTT AGCCGCTTGG CCAATTATAC TAATCTGACA
    CAAGGAGCAA AGGAACATGA AGAGGCAGAG AACATCACTG AAGGGAAAAA GAAGCCCACC
    AAGACTCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT
    GGAGTGATCC TGTTTCTGCG CCTCACATGG GTGGTGGGCA CAGCTGGAGT TCTGCAGGCC
    TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACGATGTTGA CTGCTATCTC CATGAGTGCC
    ATTGCCACTA ATGGAGTGGT GCCAGCTGGA GGCTCATACT TCATGATTTC CCGAGCACTG
    GGCCCAGAGT TTGGTGGAGC TGTTGGCCTC TGCTTCTATC TTGGTACCAC ATTTGCAGCA
    GCTATGTATA TCCTTGGTGC CATTGAAATA TTTCTGGTAT ATATCGTCCC TCGAGCTGCC
    ATCTTTCAGA GTGACGATGC ACTCAAGGAG TCAGCAGCCA TGCTAAATAA CATGCGTGTC
    TATGGGACAG CCTTCTTGGT CCTCATGGTA TTGGTGGTAT TCATCGGCGT ACGCTATGTG
    AACAAGTTTG CCTCACTCTT TCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTACGCT
    GGAGCCATCA AGTCTTCTTT CGCACCTCCA CACTTCCCGG TCTGCATGCT GGGCAACCGC
    ACCCTTTCAT CAAGACATAT TGACGTTTGC TCAAAGACCA AGGAAGTTAA CAACATGACA
    GTGCCGTCAA AGCTGTGGGG CTTCTTCTGT AACTCAAGTC AGTTTTTCAA CGCCACTTGC
    GATGAGTACT TTGTTCACAA TAATGTCACT TCGATCCAGG GCATCCCTGG ATTGGCTAGC
    GGTGTCATTA CAGAGAATCT TTGGAGTAAT TATCTACCAA AGGGAGAGAT CATTGAGAAG
    CCCTCAGCCA AATCTTCCGA AGTCTTAGGC AGCTTAAACC ATGAGTATGT CCTTGTGGAC
    ATCACCACCT CTTTCACTCT TCTGGTGGGC ATCTTCTTTC CCTCTGTCAC AGGCATCATG
    GCAGGATCAA ACAGATCTGG GGATCTGAAA GATGCTCAAA AGTCCATTCC CATTGGCACT
    ATCCTCGCCA TCCTGACCAC CTCCTTTGTC TATTTAAGCA ACGTTGTCCT TTTTGGTGCA
    TGTATTGAAG GTGTTGTCCT CAGAGACAAG TTTGGAGATG CTGTGAAAGG TAATCTGGTG
    GTAGGAACCT TATCTTGGCC ATCCCCGTGG GTGATTGTTA TTGGCTCCTT CTTCTCCACA
    TGTGGGGCTG GACTTCAGAG TCTCACAGGA GCACCGAGGC TGCTACAGGC TATAGCCAAG
    GATAACATTA TACCCTTTCT GAGGGTTTTT GGTCACAGCA AAGCCAATGG GGAACCTACC
    TGGGCTTTAC TTCTGACTGC TGCCATTGCA GAACTGGGAA TCCTTATTGC CTCCCTGGAT
    CTCGTGGCTC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT GAATTTGGCA
    TGTGCCTTGC AAACATTGCT TCGAACACCC AACTGGAGAC CCCGGTTCCG CTACTACCAC
    TGGGCACTCT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG
    TATTATGCCA TCGTAGCTAT GGTAATAGCT GGTATGATCT ACAAATACAT CGAGTACCAA
    GGAGCTGAGA AAGAATGGGG TGATGGTATC CGCGGGCTGT CCCTCAGTGC AGCCCGATTT
    GCTTTGCTTA GGCTGGAGGA AGGACCACCA CACACTAAAA ACTGGAGGCC TCAGTTGCTT
    GTATTGCTGA AACTAGATGA AGACCTACAC GTCAAGCATC CTCGTCTCCT CACCTTTGCT
    TCACAGCTTA AGGCAGGAAA GGGTCTCACA ATTGTGGGCT CTGTCATCGT CGGGAACTTC
    CTGGAGAACT ATGGTGAAGC GTTAGCTGCT GAGCAGACCA TAAAGCATTT AATGGAGGCA
    GAGAAGGTGA AAGGGTTCTC ACAGCTGGTG GTGGCCGCCA AACTGAGGGA GGGCATTTCC
    CACCTCATCC AGTCGTGTGG CCTTGGGGGC ATGAAGCACA ACACAGTGGT GATGGGATGG
    CCAAATGGCT GGCGCCAGAG TGAAGATGCT CGAGCTTGGA AGACTTTTAT TGGCACAGTT
    CGAGTGACAA CTGCTGCCCA TCTGGCCCTG CTGGTGGCCA AAAACATCTC CTTCTTTCCG
    AGCAATGTGG AGCAGTTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT ACATGATGGG
    GGGATGCTCA TGCTATTACC ATTCCTACTG AAACAGCATA AGGTGTGGCG AAAATGCAGC
    ATACGGATCT TCACAGTAGC CCAGCTAGAA GACAACAGTA TTCAGATGAA GAAGGACCTG
    GCCACCTTCT TGTATCACCT TCGCATTGAG GCAGAGGTGG AAGTGGTAGA GATGCATGAC
    AGTGACATAT CCGCATACAC TTACGAGCGC ACCCTGATGA TGGAGCAGAG GTCACAGATG
    CTGCGGCACA TGCGCCTGTC CAAGACGGAG CGAGACCGAG AGGCACAGCT GGTGAAAGAT
    CGGAACTCAA TGCTACGACT GACCAGCATC GGCTCTGACG AGGATGACGA GACAGAGACT
    TATCAGGAGA AGGTGCACAT GACGTGGACG AAAGACAAGT ATATGGCGTC TCGGGGACAA
    AAGGCCAAGT CAATGGAAGG GTTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT
    GTGAGGCGGA TGCATACAGC AGTGAAGCTC AATGAGGTTA TAGTTAACAA GTCCCATGAA
    GCAAAGCTGG TTTTGTTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGACGAAAAC
    TACATGGAGT TCCTGGAAGT ACTCACTGAA GGACTAGAGC GAGTCCTTCT TGTCCGAGGT
    GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23092
    Clone ID Related Accession (Same CDS sequence) XM_005211495.3 , XM_005211495.4
    Accession Version XM_005211495.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211495.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
    AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT
    TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG
    AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC
    CGGACATCTA ACCCCCAGGA TGTTACTGAG GATGATGGAC ATAAGAAAGC GCGAAATGCT
    TATCTTAACA ATTCCAATTA TGAAGAAGGA GATGAATATT TTGATAAGAA TTTGGCACTC
    TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTTAGCCG CTTGGCCAAT
    TATACTAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG
    AAAAAGAAGC CCACCAAGAC TCCCCAAATG GGTACCTTCA TGGGTGTCTA CCTCCCATGT
    CTACAAAATA TTTTTGGAGT GATCCTGTTT CTGCGCCTCA CATGGGTGGT GGGCACAGCT
    GGAGTTCTGC AGGCCTTTGC AATTGTCCTT ATCTGCTGCT GCTGTACGAT GTTGACTGCT
    ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG CTGGAGGCTC ATACTTCATG
    ATTTCCCGAG CACTGGGCCC AGAGTTTGGT GGAGCTGTTG GCCTCTGCTT CTATCTTGGT
    ACCACATTTG CAGCAGCTAT GTATATCCTT GGTGCCATTG AAATATTTCT GGTATATATC
    GTCCCTCGAG CTGCCATCTT TCAGAGTGAC GATGCACTCA AGGAGTCAGC AGCCATGCTA
    AATAACATGC GTGTCTATGG GACAGCCTTC TTGGTCCTCA TGGTATTGGT GGTATTCATC
    GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTCTTTCTGG CCTGTGTCAT TGTGTCCATC
    TTGGCCATCT ACGCTGGAGC CATCAAGTCT TCTTTCGCAC CTCCACACTT CCCGGTCTGC
    ATGCTGGGCA ACCGCACCCT TTCATCAAGA CATATTGACG TTTGCTCAAA GACCAAGGAA
    GTTAACAACA TGACAGTGCC GTCAAAGCTG TGGGGCTTCT TCTGTAACTC AAGTCAGTTT
    TTCAACGCCA CTTGCGATGA GTACTTTGTT CACAATAATG TCACTTCGAT CCAGGGCATC
    CCTGGATTGG CTAGCGGTGT CATTACAGAG AATCTTTGGA GTAATTATCT ACCAAAGGGA
    GAGATCATTG AGAAGCCCTC AGCCAAATCT TCCGAAGTCT TAGGCAGCTT AAACCATGAG
    TATGTCCTTG TGGACATCAC CACCTCTTTC ACTCTTCTGG TGGGCATCTT CTTTCCCTCT
    GTCACAGGCA TCATGGCAGG ATCAAACAGA TCTGGGGATC TGAAAGATGC TCAAAAGTCC
    ATTCCCATTG GCACTATCCT CGCCATCCTG ACCACCTCCT TTGTCTATTT AAGCAACGTT
    GTCCTTTTTG GTGCATGTAT TGAAGGTGTT GTCCTCAGAG ACAAGTTTGG AGATGCTGTG
    AAAGGTAATC TGGTGGTAGG AACCTTATCT TGGCCATCCC CGTGGGTGAT TGTTATTGGC
    TCCTTCTTCT CCACATGTGG GGCTGGACTT CAGAGTCTCA CAGGAGCACC GAGGCTGCTA
    CAGGCTATAG CCAAGGATAA CATTATACCC TTTCTGAGGG TTTTTGGTCA CAGCAAAGCC
    AATGGGGAAC CTACCTGGGC TTTACTTCTG ACTGCTGCCA TTGCAGAACT GGGAATCCTT
    ATTGCCTCCC TGGATCTCGT GGCTCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC
    TTTGTGAATT TGGCATGTGC CTTGCAAACA TTGCTTCGAA CACCCAACTG GAGACCCCGG
    TTCCGCTACT ACCACTGGGC ACTCTCTTTC ATGGGAATGA GTATCTGTCT GGCTCTGATG
    TTCATTTCTT CCTGGTATTA TGCCATCGTA GCTATGGTAA TAGCTGGTAT GATCTACAAA
    TACATCGAGT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG GTATCCGCGG GCTGTCCCTC
    AGTGCAGCCC GATTTGCTTT GCTTAGGCTG GAGGAAGGAC CACCACACAC TAAAAACTGG
    AGGCCTCAGT TGCTTGTATT GCTGAAACTA GATGAAGACC TACACGTCAA GCATCCTCGT
    CTCCTCACCT TTGCTTCACA GCTTAAGGCA GGAAAGGGTC TCACAATTGT GGGCTCTGTC
    ATCGTCGGGA ACTTCCTGGA GAACTATGGT GAAGCGTTAG CTGCTGAGCA GACCATAAAG
    CATTTAATGG AGGCAGAGAA GGTGAAAGGG TTCTCACAGC TGGTGGTGGC CGCCAAACTG
    AGGGAGGGCA TTTCCCACCT CATCCAGTCG TGTGGCCTTG GGGGCATGAA GCACAACACA
    GTGGTGATGG GATGGCCAAA TGGCTGGCGC CAGAGTGAAG ATGCTCGAGC TTGGAAGACT
    TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTGG CCCTGCTGGT GGCCAAAAAC
    ATCTCCTTCT TTCCGAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG
    ATTGTACATG ATGGGGGGAT GCTCATGCTA TTACCATTCC TACTGAAACA GCATAAGGTG
    TGGCGAAAAT GCAGCATACG GATCTTCACA GTAGCCCAGC TAGAAGACAA CAGTATTCAG
    ATGAAGAAGG ACCTGGCCAC CTTCTTGTAT CACCTTCGCA TTGAGGCAGA GGTGGAAGTG
    GTAGAGATGC ATGACAGTGA CATATCCGCA TACACTTACG AGCGCACCCT GATGATGGAG
    CAGAGGTCAC AGATGCTGCG GCACATGCGC CTGTCCAAGA CGGAGCGAGA CCGAGAGGCA
    CAGCTGGTGA AAGATCGGAA CTCAATGCTA CGACTGACCA GCATCGGCTC TGACGAGGAT
    GACGAGACAG AGACTTATCA GGAGAAGGTG CACATGACGT GGACGAAAGA CAAGTATATG
    GCGTCTCGGG GACAAAAGGC CAAGTCAATG GAAGGGTTCC AGGACCTGCT TAACATGCGT
    CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA AGCTCAATGA GGTTATAGTT
    AACAAGTCCC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGGCCACC CCGAAACCCT
    GAGGGTGACG AAAACTACAT GGAGTTCCTG GAAGTACTCA CTGAAGGACT AGAGCGAGTC
    CTTCTTGTCC GAGGTGGTGG CAGTGAAGTG ATCACCATTT ATTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211552.1
    CDS1121..4528
    Translation

    Target ORF information:

    RefSeq Version XM_005211495.3
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211495.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
    AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT
    TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG
    AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC
    CGGACATCTA ACCCCCAGGA TGTTACTGAG GATGATGGAC ATAAGAAAGC GCGAAATGCT
    TATCTTAACA ATTCCAATTA TGAAGAAGGA GATGAATATT TTGATAAGAA TTTGGCACTC
    TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTTAGCCG CTTGGCCAAT
    TATACTAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG
    AAAAAGAAGC CCACCAAGAC TCCCCAAATG GGTACCTTCA TGGGTGTCTA CCTCCCATGT
    CTACAAAATA TTTTTGGAGT GATCCTGTTT CTGCGCCTCA CATGGGTGGT GGGCACAGCT
    GGAGTTCTGC AGGCCTTTGC AATTGTCCTT ATCTGCTGCT GCTGTACGAT GTTGACTGCT
    ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG CTGGAGGCTC ATACTTCATG
    ATTTCCCGAG CACTGGGCCC AGAGTTTGGT GGAGCTGTTG GCCTCTGCTT CTATCTTGGT
    ACCACATTTG CAGCAGCTAT GTATATCCTT GGTGCCATTG AAATATTTCT GGTATATATC
    GTCCCTCGAG CTGCCATCTT TCAGAGTGAC GATGCACTCA AGGAGTCAGC AGCCATGCTA
    AATAACATGC GTGTCTATGG GACAGCCTTC TTGGTCCTCA TGGTATTGGT GGTATTCATC
    GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTCTTTCTGG CCTGTGTCAT TGTGTCCATC
    TTGGCCATCT ACGCTGGAGC CATCAAGTCT TCTTTCGCAC CTCCACACTT CCCGGTCTGC
    ATGCTGGGCA ACCGCACCCT TTCATCAAGA CATATTGACG TTTGCTCAAA GACCAAGGAA
    GTTAACAACA TGACAGTGCC GTCAAAGCTG TGGGGCTTCT TCTGTAACTC AAGTCAGTTT
    TTCAACGCCA CTTGCGATGA GTACTTTGTT CACAATAATG TCACTTCGAT CCAGGGCATC
    CCTGGATTGG CTAGCGGTGT CATTACAGAG AATCTTTGGA GTAATTATCT ACCAAAGGGA
    GAGATCATTG AGAAGCCCTC AGCCAAATCT TCCGAAGTCT TAGGCAGCTT AAACCATGAG
    TATGTCCTTG TGGACATCAC CACCTCTTTC ACTCTTCTGG TGGGCATCTT CTTTCCCTCT
    GTCACAGGCA TCATGGCAGG ATCAAACAGA TCTGGGGATC TGAAAGATGC TCAAAAGTCC
    ATTCCCATTG GCACTATCCT CGCCATCCTG ACCACCTCCT TTGTCTATTT AAGCAACGTT
    GTCCTTTTTG GTGCATGTAT TGAAGGTGTT GTCCTCAGAG ACAAGTTTGG AGATGCTGTG
    AAAGGTAATC TGGTGGTAGG AACCTTATCT TGGCCATCCC CGTGGGTGAT TGTTATTGGC
    TCCTTCTTCT CCACATGTGG GGCTGGACTT CAGAGTCTCA CAGGAGCACC GAGGCTGCTA
    CAGGCTATAG CCAAGGATAA CATTATACCC TTTCTGAGGG TTTTTGGTCA CAGCAAAGCC
    AATGGGGAAC CTACCTGGGC TTTACTTCTG ACTGCTGCCA TTGCAGAACT GGGAATCCTT
    ATTGCCTCCC TGGATCTCGT GGCTCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC
    TTTGTGAATT TGGCATGTGC CTTGCAAACA TTGCTTCGAA CACCCAACTG GAGACCCCGG
    TTCCGCTACT ACCACTGGGC ACTCTCTTTC ATGGGAATGA GTATCTGTCT GGCTCTGATG
    TTCATTTCTT CCTGGTATTA TGCCATCGTA GCTATGGTAA TAGCTGGTAT GATCTACAAA
    TACATCGAGT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG GTATCCGCGG GCTGTCCCTC
    AGTGCAGCCC GATTTGCTTT GCTTAGGCTG GAGGAAGGAC CACCACACAC TAAAAACTGG
    AGGCCTCAGT TGCTTGTATT GCTGAAACTA GATGAAGACC TACACGTCAA GCATCCTCGT
    CTCCTCACCT TTGCTTCACA GCTTAAGGCA GGAAAGGGTC TCACAATTGT GGGCTCTGTC
    ATCGTCGGGA ACTTCCTGGA GAACTATGGT GAAGCGTTAG CTGCTGAGCA GACCATAAAG
    CATTTAATGG AGGCAGAGAA GGTGAAAGGG TTCTCACAGC TGGTGGTGGC CGCCAAACTG
    AGGGAGGGCA TTTCCCACCT CATCCAGTCG TGTGGCCTTG GGGGCATGAA GCACAACACA
    GTGGTGATGG GATGGCCAAA TGGCTGGCGC CAGAGTGAAG ATGCTCGAGC TTGGAAGACT
    TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTGG CCCTGCTGGT GGCCAAAAAC
    ATCTCCTTCT TTCCGAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG
    ATTGTACATG ATGGGGGGAT GCTCATGCTA TTACCATTCC TACTGAAACA GCATAAGGTG
    TGGCGAAAAT GCAGCATACG GATCTTCACA GTAGCCCAGC TAGAAGACAA CAGTATTCAG
    ATGAAGAAGG ACCTGGCCAC CTTCTTGTAT CACCTTCGCA TTGAGGCAGA GGTGGAAGTG
    GTAGAGATGC ATGACAGTGA CATATCCGCA TACACTTACG AGCGCACCCT GATGATGGAG
    CAGAGGTCAC AGATGCTGCG GCACATGCGC CTGTCCAAGA CGGAGCGAGA CCGAGAGGCA
    CAGCTGGTGA AAGATCGGAA CTCAATGCTA CGACTGACCA GCATCGGCTC TGACGAGGAT
    GACGAGACAG AGACTTATCA GGAGAAGGTG CACATGACGT GGACGAAAGA CAAGTATATG
    GCGTCTCGGG GACAAAAGGC CAAGTCAATG GAAGGGTTCC AGGACCTGCT TAACATGCGT
    CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA AGCTCAATGA GGTTATAGTT
    AACAAGTCCC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGGCCACC CCGAAACCCT
    GAGGGTGACG AAAACTACAT GGAGTTCCTG GAAGTACTCA CTGAAGGACT AGAGCGAGTC
    CTTCTTGTCC GAGGTGGTGG CAGTGAAGTG ATCACCATTT ATTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23092
    Clone ID Related Accession (Same CDS sequence) XM_005211495.3 , XM_005211495.4
    Accession Version XM_005211495.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211495.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
    AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT
    TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG
    AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC
    CGGACATCTA ACCCCCAGGA TGTTACTGAG GATGATGGAC ATAAGAAAGC GCGAAATGCT
    TATCTTAACA ATTCCAATTA TGAAGAAGGA GATGAATATT TTGATAAGAA TTTGGCACTC
    TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTTAGCCG CTTGGCCAAT
    TATACTAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG
    AAAAAGAAGC CCACCAAGAC TCCCCAAATG GGTACCTTCA TGGGTGTCTA CCTCCCATGT
    CTACAAAATA TTTTTGGAGT GATCCTGTTT CTGCGCCTCA CATGGGTGGT GGGCACAGCT
    GGAGTTCTGC AGGCCTTTGC AATTGTCCTT ATCTGCTGCT GCTGTACGAT GTTGACTGCT
    ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG CTGGAGGCTC ATACTTCATG
    ATTTCCCGAG CACTGGGCCC AGAGTTTGGT GGAGCTGTTG GCCTCTGCTT CTATCTTGGT
    ACCACATTTG CAGCAGCTAT GTATATCCTT GGTGCCATTG AAATATTTCT GGTATATATC
    GTCCCTCGAG CTGCCATCTT TCAGAGTGAC GATGCACTCA AGGAGTCAGC AGCCATGCTA
    AATAACATGC GTGTCTATGG GACAGCCTTC TTGGTCCTCA TGGTATTGGT GGTATTCATC
    GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTCTTTCTGG CCTGTGTCAT TGTGTCCATC
    TTGGCCATCT ACGCTGGAGC CATCAAGTCT TCTTTCGCAC CTCCACACTT CCCGGTCTGC
    ATGCTGGGCA ACCGCACCCT TTCATCAAGA CATATTGACG TTTGCTCAAA GACCAAGGAA
    GTTAACAACA TGACAGTGCC GTCAAAGCTG TGGGGCTTCT TCTGTAACTC AAGTCAGTTT
    TTCAACGCCA CTTGCGATGA GTACTTTGTT CACAATAATG TCACTTCGAT CCAGGGCATC
    CCTGGATTGG CTAGCGGTGT CATTACAGAG AATCTTTGGA GTAATTATCT ACCAAAGGGA
    GAGATCATTG AGAAGCCCTC AGCCAAATCT TCCGAAGTCT TAGGCAGCTT AAACCATGAG
    TATGTCCTTG TGGACATCAC CACCTCTTTC ACTCTTCTGG TGGGCATCTT CTTTCCCTCT
    GTCACAGGCA TCATGGCAGG ATCAAACAGA TCTGGGGATC TGAAAGATGC TCAAAAGTCC
    ATTCCCATTG GCACTATCCT CGCCATCCTG ACCACCTCCT TTGTCTATTT AAGCAACGTT
    GTCCTTTTTG GTGCATGTAT TGAAGGTGTT GTCCTCAGAG ACAAGTTTGG AGATGCTGTG
    AAAGGTAATC TGGTGGTAGG AACCTTATCT TGGCCATCCC CGTGGGTGAT TGTTATTGGC
    TCCTTCTTCT CCACATGTGG GGCTGGACTT CAGAGTCTCA CAGGAGCACC GAGGCTGCTA
    CAGGCTATAG CCAAGGATAA CATTATACCC TTTCTGAGGG TTTTTGGTCA CAGCAAAGCC
    AATGGGGAAC CTACCTGGGC TTTACTTCTG ACTGCTGCCA TTGCAGAACT GGGAATCCTT
    ATTGCCTCCC TGGATCTCGT GGCTCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC
    TTTGTGAATT TGGCATGTGC CTTGCAAACA TTGCTTCGAA CACCCAACTG GAGACCCCGG
    TTCCGCTACT ACCACTGGGC ACTCTCTTTC ATGGGAATGA GTATCTGTCT GGCTCTGATG
    TTCATTTCTT CCTGGTATTA TGCCATCGTA GCTATGGTAA TAGCTGGTAT GATCTACAAA
    TACATCGAGT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG GTATCCGCGG GCTGTCCCTC
    AGTGCAGCCC GATTTGCTTT GCTTAGGCTG GAGGAAGGAC CACCACACAC TAAAAACTGG
    AGGCCTCAGT TGCTTGTATT GCTGAAACTA GATGAAGACC TACACGTCAA GCATCCTCGT
    CTCCTCACCT TTGCTTCACA GCTTAAGGCA GGAAAGGGTC TCACAATTGT GGGCTCTGTC
    ATCGTCGGGA ACTTCCTGGA GAACTATGGT GAAGCGTTAG CTGCTGAGCA GACCATAAAG
    CATTTAATGG AGGCAGAGAA GGTGAAAGGG TTCTCACAGC TGGTGGTGGC CGCCAAACTG
    AGGGAGGGCA TTTCCCACCT CATCCAGTCG TGTGGCCTTG GGGGCATGAA GCACAACACA
    GTGGTGATGG GATGGCCAAA TGGCTGGCGC CAGAGTGAAG ATGCTCGAGC TTGGAAGACT
    TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTGG CCCTGCTGGT GGCCAAAAAC
    ATCTCCTTCT TTCCGAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG
    ATTGTACATG ATGGGGGGAT GCTCATGCTA TTACCATTCC TACTGAAACA GCATAAGGTG
    TGGCGAAAAT GCAGCATACG GATCTTCACA GTAGCCCAGC TAGAAGACAA CAGTATTCAG
    ATGAAGAAGG ACCTGGCCAC CTTCTTGTAT CACCTTCGCA TTGAGGCAGA GGTGGAAGTG
    GTAGAGATGC ATGACAGTGA CATATCCGCA TACACTTACG AGCGCACCCT GATGATGGAG
    CAGAGGTCAC AGATGCTGCG GCACATGCGC CTGTCCAAGA CGGAGCGAGA CCGAGAGGCA
    CAGCTGGTGA AAGATCGGAA CTCAATGCTA CGACTGACCA GCATCGGCTC TGACGAGGAT
    GACGAGACAG AGACTTATCA GGAGAAGGTG CACATGACGT GGACGAAAGA CAAGTATATG
    GCGTCTCGGG GACAAAAGGC CAAGTCAATG GAAGGGTTCC AGGACCTGCT TAACATGCGT
    CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA AGCTCAATGA GGTTATAGTT
    AACAAGTCCC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGGCCACC CCGAAACCCT
    GAGGGTGACG AAAACTACAT GGAGTTCCTG GAAGTACTCA CTGAAGGACT AGAGCGAGTC
    CTTCTTGTCC GAGGTGGTGG CAGTGAAGTG ATCACCATTT ATTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211552.1
    CDS221..3628
    Translation

    Target ORF information:

    RefSeq Version XM_005211495.4
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 member 6 (SLC12A6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211495.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
    AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT
    TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG
    AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC
    CGGACATCTA ACCCCCAGGA TGTTACTGAG GATGATGGAC ATAAGAAAGC GCGAAATGCT
    TATCTTAACA ATTCCAATTA TGAAGAAGGA GATGAATATT TTGATAAGAA TTTGGCACTC
    TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTTAGCCG CTTGGCCAAT
    TATACTAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG
    AAAAAGAAGC CCACCAAGAC TCCCCAAATG GGTACCTTCA TGGGTGTCTA CCTCCCATGT
    CTACAAAATA TTTTTGGAGT GATCCTGTTT CTGCGCCTCA CATGGGTGGT GGGCACAGCT
    GGAGTTCTGC AGGCCTTTGC AATTGTCCTT ATCTGCTGCT GCTGTACGAT GTTGACTGCT
    ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG CTGGAGGCTC ATACTTCATG
    ATTTCCCGAG CACTGGGCCC AGAGTTTGGT GGAGCTGTTG GCCTCTGCTT CTATCTTGGT
    ACCACATTTG CAGCAGCTAT GTATATCCTT GGTGCCATTG AAATATTTCT GGTATATATC
    GTCCCTCGAG CTGCCATCTT TCAGAGTGAC GATGCACTCA AGGAGTCAGC AGCCATGCTA
    AATAACATGC GTGTCTATGG GACAGCCTTC TTGGTCCTCA TGGTATTGGT GGTATTCATC
    GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTCTTTCTGG CCTGTGTCAT TGTGTCCATC
    TTGGCCATCT ACGCTGGAGC CATCAAGTCT TCTTTCGCAC CTCCACACTT CCCGGTCTGC
    ATGCTGGGCA ACCGCACCCT TTCATCAAGA CATATTGACG TTTGCTCAAA GACCAAGGAA
    GTTAACAACA TGACAGTGCC GTCAAAGCTG TGGGGCTTCT TCTGTAACTC AAGTCAGTTT
    TTCAACGCCA CTTGCGATGA GTACTTTGTT CACAATAATG TCACTTCGAT CCAGGGCATC
    CCTGGATTGG CTAGCGGTGT CATTACAGAG AATCTTTGGA GTAATTATCT ACCAAAGGGA
    GAGATCATTG AGAAGCCCTC AGCCAAATCT TCCGAAGTCT TAGGCAGCTT AAACCATGAG
    TATGTCCTTG TGGACATCAC CACCTCTTTC ACTCTTCTGG TGGGCATCTT CTTTCCCTCT
    GTCACAGGCA TCATGGCAGG ATCAAACAGA TCTGGGGATC TGAAAGATGC TCAAAAGTCC
    ATTCCCATTG GCACTATCCT CGCCATCCTG ACCACCTCCT TTGTCTATTT AAGCAACGTT
    GTCCTTTTTG GTGCATGTAT TGAAGGTGTT GTCCTCAGAG ACAAGTTTGG AGATGCTGTG
    AAAGGTAATC TGGTGGTAGG AACCTTATCT TGGCCATCCC CGTGGGTGAT TGTTATTGGC
    TCCTTCTTCT CCACATGTGG GGCTGGACTT CAGAGTCTCA CAGGAGCACC GAGGCTGCTA
    CAGGCTATAG CCAAGGATAA CATTATACCC TTTCTGAGGG TTTTTGGTCA CAGCAAAGCC
    AATGGGGAAC CTACCTGGGC TTTACTTCTG ACTGCTGCCA TTGCAGAACT GGGAATCCTT
    ATTGCCTCCC TGGATCTCGT GGCTCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC
    TTTGTGAATT TGGCATGTGC CTTGCAAACA TTGCTTCGAA CACCCAACTG GAGACCCCGG
    TTCCGCTACT ACCACTGGGC ACTCTCTTTC ATGGGAATGA GTATCTGTCT GGCTCTGATG
    TTCATTTCTT CCTGGTATTA TGCCATCGTA GCTATGGTAA TAGCTGGTAT GATCTACAAA
    TACATCGAGT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG GTATCCGCGG GCTGTCCCTC
    AGTGCAGCCC GATTTGCTTT GCTTAGGCTG GAGGAAGGAC CACCACACAC TAAAAACTGG
    AGGCCTCAGT TGCTTGTATT GCTGAAACTA GATGAAGACC TACACGTCAA GCATCCTCGT
    CTCCTCACCT TTGCTTCACA GCTTAAGGCA GGAAAGGGTC TCACAATTGT GGGCTCTGTC
    ATCGTCGGGA ACTTCCTGGA GAACTATGGT GAAGCGTTAG CTGCTGAGCA GACCATAAAG
    CATTTAATGG AGGCAGAGAA GGTGAAAGGG TTCTCACAGC TGGTGGTGGC CGCCAAACTG
    AGGGAGGGCA TTTCCCACCT CATCCAGTCG TGTGGCCTTG GGGGCATGAA GCACAACACA
    GTGGTGATGG GATGGCCAAA TGGCTGGCGC CAGAGTGAAG ATGCTCGAGC TTGGAAGACT
    TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTGG CCCTGCTGGT GGCCAAAAAC
    ATCTCCTTCT TTCCGAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG
    ATTGTACATG ATGGGGGGAT GCTCATGCTA TTACCATTCC TACTGAAACA GCATAAGGTG
    TGGCGAAAAT GCAGCATACG GATCTTCACA GTAGCCCAGC TAGAAGACAA CAGTATTCAG
    ATGAAGAAGG ACCTGGCCAC CTTCTTGTAT CACCTTCGCA TTGAGGCAGA GGTGGAAGTG
    GTAGAGATGC ATGACAGTGA CATATCCGCA TACACTTACG AGCGCACCCT GATGATGGAG
    CAGAGGTCAC AGATGCTGCG GCACATGCGC CTGTCCAAGA CGGAGCGAGA CCGAGAGGCA
    CAGCTGGTGA AAGATCGGAA CTCAATGCTA CGACTGACCA GCATCGGCTC TGACGAGGAT
    GACGAGACAG AGACTTATCA GGAGAAGGTG CACATGACGT GGACGAAAGA CAAGTATATG
    GCGTCTCGGG GACAAAAGGC CAAGTCAATG GAAGGGTTCC AGGACCTGCT TAACATGCGT
    CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA AGCTCAATGA GGTTATAGTT
    AACAAGTCCC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGGCCACC CCGAAACCCT
    GAGGGTGACG AAAACTACAT GGAGTTCCTG GAAGTACTCA CTGAAGGACT AGAGCGAGTC
    CTTCTTGTCC GAGGTGGTGG CAGTGAAGTG ATCACCATTT ATTCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23094
    Clone ID Related Accession (Same CDS sequence) XM_005211497.3 , XM_005211497.4
    Accession Version XM_005211497.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACACT TCACAGTGAC TAAAGTAGAG GATACGGAGG AGGGGGCAGC AGCTCCCGTC 
    TCCCAGGAGG GGGAACCCAG CTTAGCAGAA ATAAAAGCCC CGATTCAGCA TTCAGATGAA
    CCAGACCCGA GTCAGAACTC CATCACAGGG GAACACAGCC AGCTGTTAGA TGATGGACAT
    AAGAAAGCGC GAAATGCTTA TCTTAACAAT TCCAATTATG AAGAAGGAGA TGAATATTTT
    GATAAGAATT TGGCACTCTT TGAGGAAGAA ATGGACACCA GACCAAAGGT GTCTTCTCTC
    CTTAGCCGCT TGGCCAATTA TACTAATCTG ACACAAGGAG CAAAGGAACA TGAAGAGGCA
    GAGAACATCA CTGAAGGGAA AAAGAAGCCC ACCAAGACTC CCCAAATGGG TACCTTCATG
    GGTGTCTACC TCCCATGTCT ACAAAATATT TTTGGAGTGA TCCTGTTTCT GCGCCTCACA
    TGGGTGGTGG GCACAGCTGG AGTTCTGCAG GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC
    TGTACGATGT TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT
    GGAGGCTCAT ACTTCATGAT TTCCCGAGCA CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC
    CTCTGCTTCT ATCTTGGTAC CACATTTGCA GCAGCTATGT ATATCCTTGG TGCCATTGAA
    ATATTTCTGG TATATATCGT CCCTCGAGCT GCCATCTTTC AGAGTGACGA TGCACTCAAG
    GAGTCAGCAG CCATGCTAAA TAACATGCGT GTCTATGGGA CAGCCTTCTT GGTCCTCATG
    GTATTGGTGG TATTCATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT CTTTCTGGCC
    TGTGTCATTG TGTCCATCTT GGCCATCTAC GCTGGAGCCA TCAAGTCTTC TTTCGCACCT
    CCACACTTCC CGGTCTGCAT GCTGGGCAAC CGCACCCTTT CATCAAGACA TATTGACGTT
    TGCTCAAAGA CCAAGGAAGT TAACAACATG ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC
    TGTAACTCAA GTCAGTTTTT CAACGCCACT TGCGATGAGT ACTTTGTTCA CAATAATGTC
    ACTTCGATCC AGGGCATCCC TGGATTGGCT AGCGGTGTCA TTACAGAGAA TCTTTGGAGT
    AATTATCTAC CAAAGGGAGA GATCATTGAG AAGCCCTCAG CCAAATCTTC CGAAGTCTTA
    GGCAGCTTAA ACCATGAGTA TGTCCTTGTG GACATCACCA CCTCTTTCAC TCTTCTGGTG
    GGCATCTTCT TTCCCTCTGT CACAGGCATC ATGGCAGGAT CAAACAGATC TGGGGATCTG
    AAAGATGCTC AAAAGTCCAT TCCCATTGGC ACTATCCTCG CCATCCTGAC CACCTCCTTT
    GTCTATTTAA GCAACGTTGT CCTTTTTGGT GCATGTATTG AAGGTGTTGT CCTCAGAGAC
    AAGTTTGGAG ATGCTGTGAA AGGTAATCTG GTGGTAGGAA CCTTATCTTG GCCATCCCCG
    TGGGTGATTG TTATTGGCTC CTTCTTCTCC ACATGTGGGG CTGGACTTCA GAGTCTCACA
    GGAGCACCGA GGCTGCTACA GGCTATAGCC AAGGATAACA TTATACCCTT TCTGAGGGTT
    TTTGGTCACA GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTGAC TGCTGCCATT
    GCAGAACTGG GAATCCTTAT TGCCTCCCTG GATCTCGTGG CTCCAATTCT TTCCATGTTT
    TTTCTCATGT GTTACCTCTT TGTGAATTTG GCATGTGCCT TGCAAACATT GCTTCGAACA
    CCCAACTGGA GACCCCGGTT CCGCTACTAC CACTGGGCAC TCTCTTTCAT GGGAATGAGT
    ATCTGTCTGG CTCTGATGTT CATTTCTTCC TGGTATTATG CCATCGTAGC TATGGTAATA
    GCTGGTATGA TCTACAAATA CATCGAGTAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT
    ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA TTTGCTTTGC TTAGGCTGGA GGAAGGACCA
    CCACACACTA AAAACTGGAG GCCTCAGTTG CTTGTATTGC TGAAACTAGA TGAAGACCTA
    CACGTCAAGC ATCCTCGTCT CCTCACCTTT GCTTCACAGC TTAAGGCAGG AAAGGGTCTC
    ACAATTGTGG GCTCTGTCAT CGTCGGGAAC TTCCTGGAGA ACTATGGTGA AGCGTTAGCT
    GCTGAGCAGA CCATAAAGCA TTTAATGGAG GCAGAGAAGG TGAAAGGGTT CTCACAGCTG
    GTGGTGGCCG CCAAACTGAG GGAGGGCATT TCCCACCTCA TCCAGTCGTG TGGCCTTGGG
    GGCATGAAGC ACAACACAGT GGTGATGGGA TGGCCAAATG GCTGGCGCCA GAGTGAAGAT
    GCTCGAGCTT GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTGGCC
    CTGCTGGTGG CCAAAAACAT CTCCTTCTTT CCGAGCAATG TGGAGCAGTT TTCTGAGGGC
    AACATTGATG TGTGGTGGAT TGTACATGAT GGGGGGATGC TCATGCTATT ACCATTCCTA
    CTGAAACAGC ATAAGGTGTG GCGAAAATGC AGCATACGGA TCTTCACAGT AGCCCAGCTA
    GAAGACAACA GTATTCAGAT GAAGAAGGAC CTGGCCACCT TCTTGTATCA CCTTCGCATT
    GAGGCAGAGG TGGAAGTGGT AGAGATGCAT GACAGTGACA TATCCGCATA CACTTACGAG
    CGCACCCTGA TGATGGAGCA GAGGTCACAG ATGCTGCGGC ACATGCGCCT GTCCAAGACG
    GAGCGAGACC GAGAGGCACA GCTGGTGAAA GATCGGAACT CAATGCTACG ACTGACCAGC
    ATCGGCTCTG ACGAGGATGA CGAGACAGAG ACTTATCAGG AGAAGGTGCA CATGACGTGG
    ACGAAAGACA AGTATATGGC GTCTCGGGGA CAAAAGGCCA AGTCAATGGA AGGGTTCCAG
    GACCTGCTTA ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAG
    CTCAATGAGG TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTGTT GAATATGCCA
    GGGCCACCCC GAAACCCTGA GGGTGACGAA AACTACATGG AGTTCCTGGA AGTACTCACT
    GAAGGACTAG AGCGAGTCCT TCTTGTCCGA GGTGGTGGCA GTGAAGTGAT CACCATTTAT
    TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211554.1
    CDS161..3466
    Translation

    Target ORF information:

    RefSeq Version XM_005211497.3
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 (potassium/chloride transporter), member 6 (SLC12A6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211497.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACACT TCACAGTGAC TAAAGTAGAG GATACGGAGG AGGGGGCAGC AGCTCCCGTC 
    TCCCAGGAGG GGGAACCCAG CTTAGCAGAA ATAAAAGCCC CGATTCAGCA TTCAGATGAA
    CCAGACCCGA GTCAGAACTC CATCACAGGG GAACACAGCC AGCTGTTAGA TGATGGACAT
    AAGAAAGCGC GAAATGCTTA TCTTAACAAT TCCAATTATG AAGAAGGAGA TGAATATTTT
    GATAAGAATT TGGCACTCTT TGAGGAAGAA ATGGACACCA GACCAAAGGT GTCTTCTCTC
    CTTAGCCGCT TGGCCAATTA TACTAATCTG ACACAAGGAG CAAAGGAACA TGAAGAGGCA
    GAGAACATCA CTGAAGGGAA AAAGAAGCCC ACCAAGACTC CCCAAATGGG TACCTTCATG
    GGTGTCTACC TCCCATGTCT ACAAAATATT TTTGGAGTGA TCCTGTTTCT GCGCCTCACA
    TGGGTGGTGG GCACAGCTGG AGTTCTGCAG GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC
    TGTACGATGT TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT
    GGAGGCTCAT ACTTCATGAT TTCCCGAGCA CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC
    CTCTGCTTCT ATCTTGGTAC CACATTTGCA GCAGCTATGT ATATCCTTGG TGCCATTGAA
    ATATTTCTGG TATATATCGT CCCTCGAGCT GCCATCTTTC AGAGTGACGA TGCACTCAAG
    GAGTCAGCAG CCATGCTAAA TAACATGCGT GTCTATGGGA CAGCCTTCTT GGTCCTCATG
    GTATTGGTGG TATTCATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT CTTTCTGGCC
    TGTGTCATTG TGTCCATCTT GGCCATCTAC GCTGGAGCCA TCAAGTCTTC TTTCGCACCT
    CCACACTTCC CGGTCTGCAT GCTGGGCAAC CGCACCCTTT CATCAAGACA TATTGACGTT
    TGCTCAAAGA CCAAGGAAGT TAACAACATG ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC
    TGTAACTCAA GTCAGTTTTT CAACGCCACT TGCGATGAGT ACTTTGTTCA CAATAATGTC
    ACTTCGATCC AGGGCATCCC TGGATTGGCT AGCGGTGTCA TTACAGAGAA TCTTTGGAGT
    AATTATCTAC CAAAGGGAGA GATCATTGAG AAGCCCTCAG CCAAATCTTC CGAAGTCTTA
    GGCAGCTTAA ACCATGAGTA TGTCCTTGTG GACATCACCA CCTCTTTCAC TCTTCTGGTG
    GGCATCTTCT TTCCCTCTGT CACAGGCATC ATGGCAGGAT CAAACAGATC TGGGGATCTG
    AAAGATGCTC AAAAGTCCAT TCCCATTGGC ACTATCCTCG CCATCCTGAC CACCTCCTTT
    GTCTATTTAA GCAACGTTGT CCTTTTTGGT GCATGTATTG AAGGTGTTGT CCTCAGAGAC
    AAGTTTGGAG ATGCTGTGAA AGGTAATCTG GTGGTAGGAA CCTTATCTTG GCCATCCCCG
    TGGGTGATTG TTATTGGCTC CTTCTTCTCC ACATGTGGGG CTGGACTTCA GAGTCTCACA
    GGAGCACCGA GGCTGCTACA GGCTATAGCC AAGGATAACA TTATACCCTT TCTGAGGGTT
    TTTGGTCACA GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTGAC TGCTGCCATT
    GCAGAACTGG GAATCCTTAT TGCCTCCCTG GATCTCGTGG CTCCAATTCT TTCCATGTTT
    TTTCTCATGT GTTACCTCTT TGTGAATTTG GCATGTGCCT TGCAAACATT GCTTCGAACA
    CCCAACTGGA GACCCCGGTT CCGCTACTAC CACTGGGCAC TCTCTTTCAT GGGAATGAGT
    ATCTGTCTGG CTCTGATGTT CATTTCTTCC TGGTATTATG CCATCGTAGC TATGGTAATA
    GCTGGTATGA TCTACAAATA CATCGAGTAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT
    ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA TTTGCTTTGC TTAGGCTGGA GGAAGGACCA
    CCACACACTA AAAACTGGAG GCCTCAGTTG CTTGTATTGC TGAAACTAGA TGAAGACCTA
    CACGTCAAGC ATCCTCGTCT CCTCACCTTT GCTTCACAGC TTAAGGCAGG AAAGGGTCTC
    ACAATTGTGG GCTCTGTCAT CGTCGGGAAC TTCCTGGAGA ACTATGGTGA AGCGTTAGCT
    GCTGAGCAGA CCATAAAGCA TTTAATGGAG GCAGAGAAGG TGAAAGGGTT CTCACAGCTG
    GTGGTGGCCG CCAAACTGAG GGAGGGCATT TCCCACCTCA TCCAGTCGTG TGGCCTTGGG
    GGCATGAAGC ACAACACAGT GGTGATGGGA TGGCCAAATG GCTGGCGCCA GAGTGAAGAT
    GCTCGAGCTT GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTGGCC
    CTGCTGGTGG CCAAAAACAT CTCCTTCTTT CCGAGCAATG TGGAGCAGTT TTCTGAGGGC
    AACATTGATG TGTGGTGGAT TGTACATGAT GGGGGGATGC TCATGCTATT ACCATTCCTA
    CTGAAACAGC ATAAGGTGTG GCGAAAATGC AGCATACGGA TCTTCACAGT AGCCCAGCTA
    GAAGACAACA GTATTCAGAT GAAGAAGGAC CTGGCCACCT TCTTGTATCA CCTTCGCATT
    GAGGCAGAGG TGGAAGTGGT AGAGATGCAT GACAGTGACA TATCCGCATA CACTTACGAG
    CGCACCCTGA TGATGGAGCA GAGGTCACAG ATGCTGCGGC ACATGCGCCT GTCCAAGACG
    GAGCGAGACC GAGAGGCACA GCTGGTGAAA GATCGGAACT CAATGCTACG ACTGACCAGC
    ATCGGCTCTG ACGAGGATGA CGAGACAGAG ACTTATCAGG AGAAGGTGCA CATGACGTGG
    ACGAAAGACA AGTATATGGC GTCTCGGGGA CAAAAGGCCA AGTCAATGGA AGGGTTCCAG
    GACCTGCTTA ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAG
    CTCAATGAGG TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTGTT GAATATGCCA
    GGGCCACCCC GAAACCCTGA GGGTGACGAA AACTACATGG AGTTCCTGGA AGTACTCACT
    GAAGGACTAG AGCGAGTCCT TCTTGTCCGA GGTGGTGGCA GTGAAGTGAT CACCATTTAT
    TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa23094
    Clone ID Related Accession (Same CDS sequence) XM_005211497.3 , XM_005211497.4
    Accession Version XM_005211497.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3306bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product solute carrier family 12 member 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACACT TCACAGTGAC TAAAGTAGAG GATACGGAGG AGGGGGCAGC AGCTCCCGTC 
    TCCCAGGAGG GGGAACCCAG CTTAGCAGAA ATAAAAGCCC CGATTCAGCA TTCAGATGAA
    CCAGACCCGA GTCAGAACTC CATCACAGGG GAACACAGCC AGCTGTTAGA TGATGGACAT
    AAGAAAGCGC GAAATGCTTA TCTTAACAAT TCCAATTATG AAGAAGGAGA TGAATATTTT
    GATAAGAATT TGGCACTCTT TGAGGAAGAA ATGGACACCA GACCAAAGGT GTCTTCTCTC
    CTTAGCCGCT TGGCCAATTA TACTAATCTG ACACAAGGAG CAAAGGAACA TGAAGAGGCA
    GAGAACATCA CTGAAGGGAA AAAGAAGCCC ACCAAGACTC CCCAAATGGG TACCTTCATG
    GGTGTCTACC TCCCATGTCT ACAAAATATT TTTGGAGTGA TCCTGTTTCT GCGCCTCACA
    TGGGTGGTGG GCACAGCTGG AGTTCTGCAG GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC
    TGTACGATGT TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT
    GGAGGCTCAT ACTTCATGAT TTCCCGAGCA CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC
    CTCTGCTTCT ATCTTGGTAC CACATTTGCA GCAGCTATGT ATATCCTTGG TGCCATTGAA
    ATATTTCTGG TATATATCGT CCCTCGAGCT GCCATCTTTC AGAGTGACGA TGCACTCAAG
    GAGTCAGCAG CCATGCTAAA TAACATGCGT GTCTATGGGA CAGCCTTCTT GGTCCTCATG
    GTATTGGTGG TATTCATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT CTTTCTGGCC
    TGTGTCATTG TGTCCATCTT GGCCATCTAC GCTGGAGCCA TCAAGTCTTC TTTCGCACCT
    CCACACTTCC CGGTCTGCAT GCTGGGCAAC CGCACCCTTT CATCAAGACA TATTGACGTT
    TGCTCAAAGA CCAAGGAAGT TAACAACATG ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC
    TGTAACTCAA GTCAGTTTTT CAACGCCACT TGCGATGAGT ACTTTGTTCA CAATAATGTC
    ACTTCGATCC AGGGCATCCC TGGATTGGCT AGCGGTGTCA TTACAGAGAA TCTTTGGAGT
    AATTATCTAC CAAAGGGAGA GATCATTGAG AAGCCCTCAG CCAAATCTTC CGAAGTCTTA
    GGCAGCTTAA ACCATGAGTA TGTCCTTGTG GACATCACCA CCTCTTTCAC TCTTCTGGTG
    GGCATCTTCT TTCCCTCTGT CACAGGCATC ATGGCAGGAT CAAACAGATC TGGGGATCTG
    AAAGATGCTC AAAAGTCCAT TCCCATTGGC ACTATCCTCG CCATCCTGAC CACCTCCTTT
    GTCTATTTAA GCAACGTTGT CCTTTTTGGT GCATGTATTG AAGGTGTTGT CCTCAGAGAC
    AAGTTTGGAG ATGCTGTGAA AGGTAATCTG GTGGTAGGAA CCTTATCTTG GCCATCCCCG
    TGGGTGATTG TTATTGGCTC CTTCTTCTCC ACATGTGGGG CTGGACTTCA GAGTCTCACA
    GGAGCACCGA GGCTGCTACA GGCTATAGCC AAGGATAACA TTATACCCTT TCTGAGGGTT
    TTTGGTCACA GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTGAC TGCTGCCATT
    GCAGAACTGG GAATCCTTAT TGCCTCCCTG GATCTCGTGG CTCCAATTCT TTCCATGTTT
    TTTCTCATGT GTTACCTCTT TGTGAATTTG GCATGTGCCT TGCAAACATT GCTTCGAACA
    CCCAACTGGA GACCCCGGTT CCGCTACTAC CACTGGGCAC TCTCTTTCAT GGGAATGAGT
    ATCTGTCTGG CTCTGATGTT CATTTCTTCC TGGTATTATG CCATCGTAGC TATGGTAATA
    GCTGGTATGA TCTACAAATA CATCGAGTAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT
    ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA TTTGCTTTGC TTAGGCTGGA GGAAGGACCA
    CCACACACTA AAAACTGGAG GCCTCAGTTG CTTGTATTGC TGAAACTAGA TGAAGACCTA
    CACGTCAAGC ATCCTCGTCT CCTCACCTTT GCTTCACAGC TTAAGGCAGG AAAGGGTCTC
    ACAATTGTGG GCTCTGTCAT CGTCGGGAAC TTCCTGGAGA ACTATGGTGA AGCGTTAGCT
    GCTGAGCAGA CCATAAAGCA TTTAATGGAG GCAGAGAAGG TGAAAGGGTT CTCACAGCTG
    GTGGTGGCCG CCAAACTGAG GGAGGGCATT TCCCACCTCA TCCAGTCGTG TGGCCTTGGG
    GGCATGAAGC ACAACACAGT GGTGATGGGA TGGCCAAATG GCTGGCGCCA GAGTGAAGAT
    GCTCGAGCTT GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTGGCC
    CTGCTGGTGG CCAAAAACAT CTCCTTCTTT CCGAGCAATG TGGAGCAGTT TTCTGAGGGC
    AACATTGATG TGTGGTGGAT TGTACATGAT GGGGGGATGC TCATGCTATT ACCATTCCTA
    CTGAAACAGC ATAAGGTGTG GCGAAAATGC AGCATACGGA TCTTCACAGT AGCCCAGCTA
    GAAGACAACA GTATTCAGAT GAAGAAGGAC CTGGCCACCT TCTTGTATCA CCTTCGCATT
    GAGGCAGAGG TGGAAGTGGT AGAGATGCAT GACAGTGACA TATCCGCATA CACTTACGAG
    CGCACCCTGA TGATGGAGCA GAGGTCACAG ATGCTGCGGC ACATGCGCCT GTCCAAGACG
    GAGCGAGACC GAGAGGCACA GCTGGTGAAA GATCGGAACT CAATGCTACG ACTGACCAGC
    ATCGGCTCTG ACGAGGATGA CGAGACAGAG ACTTATCAGG AGAAGGTGCA CATGACGTGG
    ACGAAAGACA AGTATATGGC GTCTCGGGGA CAAAAGGCCA AGTCAATGGA AGGGTTCCAG
    GACCTGCTTA ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAG
    CTCAATGAGG TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTGTT GAATATGCCA
    GGGCCACCCC GAAACCCTGA GGGTGACGAA AACTACATGG AGTTCCTGGA AGTACTCACT
    GAAGGACTAG AGCGAGTCCT TCTTGTCCGA GGTGGTGGCA GTGAAGTGAT CACCATTTAT
    TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005211554.1
    CDS219..3524
    Translation

    Target ORF information:

    RefSeq Version XM_005211497.4
    Organism Bos taurus(cattle)
    Definition Bos taurus solute carrier family 12 member 6 (SLC12A6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005211497.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACACT TCACAGTGAC TAAAGTAGAG GATACGGAGG AGGGGGCAGC AGCTCCCGTC 
    TCCCAGGAGG GGGAACCCAG CTTAGCAGAA ATAAAAGCCC CGATTCAGCA TTCAGATGAA
    CCAGACCCGA GTCAGAACTC CATCACAGGG GAACACAGCC AGCTGTTAGA TGATGGACAT
    AAGAAAGCGC GAAATGCTTA TCTTAACAAT TCCAATTATG AAGAAGGAGA TGAATATTTT
    GATAAGAATT TGGCACTCTT TGAGGAAGAA ATGGACACCA GACCAAAGGT GTCTTCTCTC
    CTTAGCCGCT TGGCCAATTA TACTAATCTG ACACAAGGAG CAAAGGAACA TGAAGAGGCA
    GAGAACATCA CTGAAGGGAA AAAGAAGCCC ACCAAGACTC CCCAAATGGG TACCTTCATG
    GGTGTCTACC TCCCATGTCT ACAAAATATT TTTGGAGTGA TCCTGTTTCT GCGCCTCACA
    TGGGTGGTGG GCACAGCTGG AGTTCTGCAG GCCTTTGCAA TTGTCCTTAT CTGCTGCTGC
    TGTACGATGT TGACTGCTAT CTCCATGAGT GCCATTGCCA CTAATGGAGT GGTGCCAGCT
    GGAGGCTCAT ACTTCATGAT TTCCCGAGCA CTGGGCCCAG AGTTTGGTGG AGCTGTTGGC
    CTCTGCTTCT ATCTTGGTAC CACATTTGCA GCAGCTATGT ATATCCTTGG TGCCATTGAA
    ATATTTCTGG TATATATCGT CCCTCGAGCT GCCATCTTTC AGAGTGACGA TGCACTCAAG
    GAGTCAGCAG CCATGCTAAA TAACATGCGT GTCTATGGGA CAGCCTTCTT GGTCCTCATG
    GTATTGGTGG TATTCATCGG CGTACGCTAT GTGAACAAGT TTGCCTCACT CTTTCTGGCC
    TGTGTCATTG TGTCCATCTT GGCCATCTAC GCTGGAGCCA TCAAGTCTTC TTTCGCACCT
    CCACACTTCC CGGTCTGCAT GCTGGGCAAC CGCACCCTTT CATCAAGACA TATTGACGTT
    TGCTCAAAGA CCAAGGAAGT TAACAACATG ACAGTGCCGT CAAAGCTGTG GGGCTTCTTC
    TGTAACTCAA GTCAGTTTTT CAACGCCACT TGCGATGAGT ACTTTGTTCA CAATAATGTC
    ACTTCGATCC AGGGCATCCC TGGATTGGCT AGCGGTGTCA TTACAGAGAA TCTTTGGAGT
    AATTATCTAC CAAAGGGAGA GATCATTGAG AAGCCCTCAG CCAAATCTTC CGAAGTCTTA
    GGCAGCTTAA ACCATGAGTA TGTCCTTGTG GACATCACCA CCTCTTTCAC TCTTCTGGTG
    GGCATCTTCT TTCCCTCTGT CACAGGCATC ATGGCAGGAT CAAACAGATC TGGGGATCTG
    AAAGATGCTC AAAAGTCCAT TCCCATTGGC ACTATCCTCG CCATCCTGAC CACCTCCTTT
    GTCTATTTAA GCAACGTTGT CCTTTTTGGT GCATGTATTG AAGGTGTTGT CCTCAGAGAC
    AAGTTTGGAG ATGCTGTGAA AGGTAATCTG GTGGTAGGAA CCTTATCTTG GCCATCCCCG
    TGGGTGATTG TTATTGGCTC CTTCTTCTCC ACATGTGGGG CTGGACTTCA GAGTCTCACA
    GGAGCACCGA GGCTGCTACA GGCTATAGCC AAGGATAACA TTATACCCTT TCTGAGGGTT
    TTTGGTCACA GCAAAGCCAA TGGGGAACCT ACCTGGGCTT TACTTCTGAC TGCTGCCATT
    GCAGAACTGG GAATCCTTAT TGCCTCCCTG GATCTCGTGG CTCCAATTCT TTCCATGTTT
    TTTCTCATGT GTTACCTCTT TGTGAATTTG GCATGTGCCT TGCAAACATT GCTTCGAACA
    CCCAACTGGA GACCCCGGTT CCGCTACTAC CACTGGGCAC TCTCTTTCAT GGGAATGAGT
    ATCTGTCTGG CTCTGATGTT CATTTCTTCC TGGTATTATG CCATCGTAGC TATGGTAATA
    GCTGGTATGA TCTACAAATA CATCGAGTAC CAAGGAGCTG AGAAAGAATG GGGTGATGGT
    ATCCGCGGGC TGTCCCTCAG TGCAGCCCGA TTTGCTTTGC TTAGGCTGGA GGAAGGACCA
    CCACACACTA AAAACTGGAG GCCTCAGTTG CTTGTATTGC TGAAACTAGA TGAAGACCTA
    CACGTCAAGC ATCCTCGTCT CCTCACCTTT GCTTCACAGC TTAAGGCAGG AAAGGGTCTC
    ACAATTGTGG GCTCTGTCAT CGTCGGGAAC TTCCTGGAGA ACTATGGTGA AGCGTTAGCT
    GCTGAGCAGA CCATAAAGCA TTTAATGGAG GCAGAGAAGG TGAAAGGGTT CTCACAGCTG
    GTGGTGGCCG CCAAACTGAG GGAGGGCATT TCCCACCTCA TCCAGTCGTG TGGCCTTGGG
    GGCATGAAGC ACAACACAGT GGTGATGGGA TGGCCAAATG GCTGGCGCCA GAGTGAAGAT
    GCTCGAGCTT GGAAGACTTT TATTGGCACA GTTCGAGTGA CAACTGCTGC CCATCTGGCC
    CTGCTGGTGG CCAAAAACAT CTCCTTCTTT CCGAGCAATG TGGAGCAGTT TTCTGAGGGC
    AACATTGATG TGTGGTGGAT TGTACATGAT GGGGGGATGC TCATGCTATT ACCATTCCTA
    CTGAAACAGC ATAAGGTGTG GCGAAAATGC AGCATACGGA TCTTCACAGT AGCCCAGCTA
    GAAGACAACA GTATTCAGAT GAAGAAGGAC CTGGCCACCT TCTTGTATCA CCTTCGCATT
    GAGGCAGAGG TGGAAGTGGT AGAGATGCAT GACAGTGACA TATCCGCATA CACTTACGAG
    CGCACCCTGA TGATGGAGCA GAGGTCACAG ATGCTGCGGC ACATGCGCCT GTCCAAGACG
    GAGCGAGACC GAGAGGCACA GCTGGTGAAA GATCGGAACT CAATGCTACG ACTGACCAGC
    ATCGGCTCTG ACGAGGATGA CGAGACAGAG ACTTATCAGG AGAAGGTGCA CATGACGTGG
    ACGAAAGACA AGTATATGGC GTCTCGGGGA CAAAAGGCCA AGTCAATGGA AGGGTTCCAG
    GACCTGCTTA ACATGCGTCC GGACCAGTCC AATGTGAGGC GGATGCATAC AGCAGTGAAG
    CTCAATGAGG TTATAGTTAA CAAGTCCCAT GAAGCAAAGC TGGTTTTGTT GAATATGCCA
    GGGCCACCCC GAAACCCTGA GGGTGACGAA AACTACATGG AGTTCCTGGA AGTACTCACT
    GAAGGACTAG AGCGAGTCCT TCTTGTCCGA GGTGGTGGCA GTGAAGTGAT CACCATTTAT
    TCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.