slc12a6 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following slc12a6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the slc12a6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa48036 XM_012965521.2
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_012965513.3
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_012965517.3
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_012965504.3
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_031901770.1
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_012965540.3
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_031901786.1
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_012965505.3
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_031901789.1
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48036 XM_012965500.3
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48037 XM_031901795.1
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48038 XM_012965533.3
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa48039 XM_031901800.1
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa24444 NM_001079108.1
Latest version!
Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29362 XM_012965505.2
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29362 XM_012965513.2
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29362 XM_012965521.1
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29362 XM_012965504.2
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29362 XM_012965500.2
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29362 XM_012965517.2
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29362 XM_018094514.1
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29362 XM_012965540.2
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29363 XM_018094520.1
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa29364 XM_012965533.2
Latest version!
Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_012965504.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820958.1
    CDS581..3982
    Translation

    Target ORF information:

    RefSeq Version XM_012965504.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965504.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_012965505.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820959.1
    CDS488..3889
    Translation

    Target ORF information:

    RefSeq Version XM_012965505.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965505.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_031901770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757630.1
    CDS649..4050
    Translation

    Target ORF information:

    RefSeq Version XM_031901770.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_012965517.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965517.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820971.1
    CDS476..3877
    Translation

    Target ORF information:

    RefSeq Version XM_012965517.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965517.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_012965540.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965540.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820994.1
    CDS313..3714
    Translation

    Target ORF information:

    RefSeq Version XM_012965540.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965540.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_012965513.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965513.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820967.1
    CDS399..3800
    Translation

    Target ORF information:

    RefSeq Version XM_012965513.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965513.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_031901789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757649.1
    CDS560..3961
    Translation

    Target ORF information:

    RefSeq Version XM_031901789.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_012965500.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965500.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820954.1
    CDS492..3893
    Translation

    Target ORF information:

    RefSeq Version XM_012965500.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965500.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_031901786.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757646.1
    CDS460..3861
    Translation

    Target ORF information:

    RefSeq Version XM_031901786.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48036
    Clone ID Related Accession (Same CDS sequence) XM_012965504.3 , XM_012965505.3 , XM_031901770.1 , XM_012965517.3 , XM_012965540.3 , XM_012965513.3 , XM_031901789.1 , XM_012965500.3 , XM_031901786.1 , XM_012965521.2
    Accession Version XM_012965521.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820975.1
    CDS268..3669
    Translation

    Target ORF information:

    RefSeq Version XM_012965521.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965521.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCA
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAGGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48037
    Clone ID Related Accession (Same CDS sequence) XM_031901795.1
    Accession Version XM_031901795.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGACATT
    GACCGCCGGA AGAGTTCAGT GCTTTATCAA AATCACACCG ATGATGAAGG GGACTCTTAT
    GACAGAAATC TGGCCCTGTT TGAGGAAGAA ATGGATACTC GACCAAAAGT TTCATCCCTC
    CTTAACCGCT TGGCTAATTA CACAAATCTG CCTCAGGGAG CAAAAGAACA TGAAGAGGAA
    AGTGAGGGCA AGAAGAAGGC TGCTAAAACC CCCCGAATGG GCACGATAAT GGGGGTTTAC
    CTCCCTTGTC TTCAGAACAT TTTTGGGGTC ATTTTATTCC TTCGACTCCC GTGGGTGGTG
    GGCACAGCAG GAGTCTTGCA TGCCTTCTGC ATTGTATTCG CCTGCTGCTG CTGTACCATG
    CTGACGGCTA TTTCTATGAG CGCCATTGCA ACAAATGGGG TGGTGCCAGC TGGTGGTGCC
    TATTTCATGA TTTCTCGAGC CCTTGGTCCA GAGTTTGGAG GTGCTGTAGG CCTGTGCTTC
    TACCTTGGAA CAACCTTTGC AACAGCTATG TACATCCTGG GGGCTATTGA GATATTCCTG
    GTGTACATTT CCCCCCAAGC AGTCATATTC CATGGAGAGG GGGTGGCAGA GGAATCAGCA
    GCAATGTTGA ATAATATGCG TGTATATGGA ACTGGATTCC TGATTATTAT GTCTATTATT
    GTGTTTGTTG GAGTGCGATA TGTAAACAAG TTGGCCTCTG TATTCCTGAC CTGTGTCATC
    ATGTCTATTC TGGCAATTTA TGCTGGGGCA CTAAAATCTG CATTTTCTCC CCCGGACTTT
    CCGATTTGCT TGCTTGGAAA CCGGACTCTG TCCAAGCATC ATTTTACCGA ATGTGCCAAG
    ATAGTGGAGA GCGACAACAT GACCATTGTC ACAGATCTGT GGAATCTATT CTGCCACCCA
    TCGGATCAGC TGAATGGAAC TTGTGATGAA TATTTTGCCC ATAATAATGT CACCTTAGTC
    CAGGGAATCC CAGGCTTAAA GAGCGGGATC ATCTCTGAAA ACCTGTGGAG CAATTACCTT
    CAAAAGGGAG ACATAATAGA AAAGGCTTCA TTGCCATCAA CTGAGTATTT GGCTGTGCAG
    TCTCAAGAGT ATGTTTTAGC TGACATTACG ACTTCTTTTA CCCTGTTGGT TGGAATATTT
    TTCCCATCTG TAACAGGAAT TATGGCTGGA TCAAATCGCT CTGGAGACCT CAAGGATGCC
    CAGAAATCGA TTCCAATTGG AACTATTCTT GCAATTCTTA CAACCTCCTT AGTCTACCTC
    AGTAATGTCA TCCTGTTTGG AGCTTCTATA GATGGAGTTG TGCTCAGAGA CAAGTTTGGG
    GATGCAGTGA AAGGAACTCT TGTTGTTGGT GCGCTTTCAT GGCCATCTCC TTGGGTCATT
    GTGATTGGAT CTTTTTTCTC TACTTGTGGA GCTGGACTTC AAAGTTTGAC TGGAGCACCT
    CGCCTGCTCC AAGCCATTGC TAAGGATGGA ATCATTCCAT TTCTGAGGGT ATTCGGACAC
    GGAAAGAGTA ATGGGGAACC GACATGGGCT CTTCTTCTAA CGGCAGTGAT TGCTGAGCTG
    GGGATCCTTA TTGCTTCTCT GGACTTGGTG GCACCTATCC TATCTATGTT CTTTCTAATG
    TGTTACCTTT TTGTAAACTT GGCATGTGGA TTACAGACCC TTCTCCATTC ACCCAACTGG
    CGCCCTCGCT TCCATTATTA TCACTGGACT TTATCTTTTC TTGGAATGGC ACTGTGTTTA
    GCCCTCATGT TTATCTCTTC CTGGTATTAT GCCCTAATTT CCATGGTAAT TGCTGGAATG
    ATCTACAAGT ACATTGAATA CCAAGGGGCC GAAAAAGAAT GGGGAGATGG AATCCGTGGA
    TTGTCCTTGA GTGCAGCACG TTTTGCTCTC CTGCGCCTGG AAGAGGGTCC ACCTCACACT
    AAGAACTGGA GACCTCAGCT ACTTGTTTTG GTGAAATTGG ATTCAGATCT CCATGTCTCT
    CAGCCTCGCC TTCTTTCCTT CGCCTCCCAG CTAAAGGCTG GTAAAGGTCT GACCATTGTG
    GGTTCAGTAC TTCTGGGAGA CTACCTAGAG AATCATGCAG AGGCCCAGGC AGCGGAGCAG
    GCACTGAAAC ACTTAATGGA GCAAGAAAAA GTAAAAGGTT TTTGCCAAGT GGTTGTGGCT
    CAGAAGCTGA AAGAGGGTCT TTCCCACCTT ATACAGTCCT GTGGTCTTGG GGGAATGAGA
    CACAACACTG TGATCATGAG CTGGCCAAGT AGCTGGAGAC AGAGTGATGA CTCACGGGCT
    TGGAAATCCT TCATCACTAC CATTCGTGTA ACCACAGCTG CCCGCCAAGC ACTTCTGGTA
    GCAAAGAATG TATCCCTGTT CCCTGGCTCC CGTGAAATAT TAGCAGAAGG GCACATTGAT
    GTTTGGTGGA TAGTACATGA TGGGGGCATG CTCATGCTTC TGCCCTTCCT CCTTAAACAA
    CACAAGGTGT GGCGGAAATG TAAGATGCGT ATTTTCACTG TGGCTCAGAT GGAAGACAAT
    AGTATTCAGA TGAAAAAGGA CTTGGCTACC TTCCTTTATC ATTTACGTAT CGCTGCTGAT
    GTTGAAGTGG TGGAGATGCA TGACAGCGAT ATATCTGCAT ACACATATGA ACGCACACTC
    ATGATGGAGC AGAGGTCACA AATGCTGAGA CAGATGCGCC TTTCAAAAAC AGATCGGGAA
    AAGGAGGCTC AGTTGGTGAA GGACAGGAAT TCAATGCTGC GTTTAACCAG TGTGGGCTCT
    GATGATGATG AAGATACAGA GGCTGTCCCA GATAGAGTGC ACATGACTTG GACCAAGGAT
    AAGCACCATG CAGTTCGTGT TGCCCAAAGC AAACCAATGC CCAGCTTTCA GGACCTTCTC
    AACATACGAC CGGACCAGTC GAATGTTCGG CGTATGCACA CAGCTGTGAA ACTTAATGAA
    GTCATTGTTA ATAAATCCCA TGATGCCAAA TTGGTTTTGC TCAACATGCC AGGACCCCCT
    CGTAACCCTC AGGGTGATGA GAATTATATG GAGTTTCTAG AGGTGCTTAC TGAGGGCTTG
    GAACACGTGC TGTTAGTGAG AGGAGGTGGA ACAGAGGTGA TCACCATTTA CTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757655.1
    CDS560..3916
    Translation

    Target ORF information:

    RefSeq Version XM_031901795.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901795.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGACATT
    GACCGCCGGA AGAGTTCAGT GCTTTATCAA AATCACACCG ATGATGAAGG GGACTCTTAT
    GACAGAAATC TGGCCCTGTT TGAGGAAGAA ATGGATACTC GACCAAAAGT TTCATCCCTC
    CTTAACCGCT TGGCTAATTA CACAAATCTG CCTCAGGGAG CAAAAGAACA TGAAGAGGAA
    AGTGAGGGCA AGAAGAAGGC TGCTAAAACC CCCCGAATGG GCACGATAAT GGGGGTTTAC
    CTCCCTTGTC TTCAGAACAT TTTTGGGGTC ATTTTATTCC TTCGACTCCC GTGGGTGGTG
    GGCACAGCAG GAGTCTTGCA TGCCTTCTGC ATTGTATTCG CCTGCTGCTG CTGTACCATG
    CTGACGGCTA TTTCTATGAG CGCCATTGCA ACAAATGGGG TGGTGCCAGC TGGTGGTGCC
    TATTTCATGA TTTCTCGAGC CCTTGGTCCA GAGTTTGGAG GTGCTGTAGG CCTGTGCTTC
    TACCTTGGAA CAACCTTTGC AACAGCTATG TACATCCTGG GGGCTATTGA GATATTCCTG
    GTGTACATTT CCCCCCAAGC AGTCATATTC CATGGAGAGG GGGTGGCAGA GGAATCAGCA
    GCAATGTTGA ATAATATGCG TGTATATGGA ACTGGATTCC TGATTATTAT GTCTATTATT
    GTGTTTGTTG GAGTGCGATA TGTAAACAAG TTGGCCTCTG TATTCCTGAC CTGTGTCATC
    ATGTCTATTC TGGCAATTTA TGCTGGGGCA CTAAAATCTG CATTTTCTCC CCCGGACTTT
    CCGATTTGCT TGCTTGGAAA CCGGACTCTG TCCAAGCATC ATTTTACCGA ATGTGCCAAG
    ATAGTGGAGA GCGACAACAT GACCATTGTC ACAGATCTGT GGAATCTATT CTGCCACCCA
    TCGGATCAGC TGAATGGAAC TTGTGATGAA TATTTTGCCC ATAATAATGT CACCTTAGTC
    CAGGGAATCC CAGGCTTAAA GAGCGGGATC ATCTCTGAAA ACCTGTGGAG CAATTACCTT
    CAAAAGGGAG ACATAATAGA AAAGGCTTCA TTGCCATCAA CTGAGTATTT GGCTGTGCAG
    TCTCAAGAGT ATGTTTTAGC TGACATTACG ACTTCTTTTA CCCTGTTGGT TGGAATATTT
    TTCCCATCTG TAACAGGAAT TATGGCTGGA TCAAATCGCT CTGGAGACCT CAAGGATGCC
    CAGAAATCGA TTCCAATTGG AACTATTCTT GCAATTCTTA CAACCTCCTT AGTCTACCTC
    AGTAATGTCA TCCTGTTTGG AGCTTCTATA GATGGAGTTG TGCTCAGAGA CAAGTTTGGG
    GATGCAGTGA AAGGAACTCT TGTTGTTGGT GCGCTTTCAT GGCCATCTCC TTGGGTCATT
    GTGATTGGAT CTTTTTTCTC TACTTGTGGA GCTGGACTTC AAAGTTTGAC TGGAGCACCT
    CGCCTGCTCC AAGCCATTGC TAAGGATGGA ATCATTCCAT TTCTGAGGGT ATTCGGACAC
    GGAAAGAGTA ATGGGGAACC GACATGGGCT CTTCTTCTAA CGGCAGTGAT TGCTGAGCTG
    GGGATCCTTA TTGCTTCTCT GGACTTGGTG GCACCTATCC TATCTATGTT CTTTCTAATG
    TGTTACCTTT TTGTAAACTT GGCATGTGGA TTACAGACCC TTCTCCATTC ACCCAACTGG
    CGCCCTCGCT TCCATTATTA TCACTGGACT TTATCTTTTC TTGGAATGGC ACTGTGTTTA
    GCCCTCATGT TTATCTCTTC CTGGTATTAT GCCCTAATTT CCATGGTAAT TGCTGGAATG
    ATCTACAAGT ACATTGAATA CCAAGGGGCC GAAAAAGAAT GGGGAGATGG AATCCGTGGA
    TTGTCCTTGA GTGCAGCACG TTTTGCTCTC CTGCGCCTGG AAGAGGGTCC ACCTCACACT
    AAGAACTGGA GACCTCAGCT ACTTGTTTTG GTGAAATTGG ATTCAGATCT CCATGTCTCT
    CAGCCTCGCC TTCTTTCCTT CGCCTCCCAG CTAAAGGCTG GTAAAGGTCT GACCATTGTG
    GGTTCAGTAC TTCTGGGAGA CTACCTAGAG AATCATGCAG AGGCCCAGGC AGCGGAGCAG
    GCACTGAAAC ACTTAATGGA GCAAGAAAAA GTAAAAGGTT TTTGCCAAGT GGTTGTGGCT
    CAGAAGCTGA AAGAGGGTCT TTCCCACCTT ATACAGTCCT GTGGTCTTGG GGGAATGAGA
    CACAACACTG TGATCATGAG CTGGCCAAGT AGCTGGAGAC AGAGTGATGA CTCACGGGCT
    TGGAAATCCT TCATCACTAC CATTCGTGTA ACCACAGCTG CCCGCCAAGC ACTTCTGGTA
    GCAAAGAATG TATCCCTGTT CCCTGGCTCC CGTGAAATAT TAGCAGAAGG GCACATTGAT
    GTTTGGTGGA TAGTACATGA TGGGGGCATG CTCATGCTTC TGCCCTTCCT CCTTAAACAA
    CACAAGGTGT GGCGGAAATG TAAGATGCGT ATTTTCACTG TGGCTCAGAT GGAAGACAAT
    AGTATTCAGA TGAAAAAGGA CTTGGCTACC TTCCTTTATC ATTTACGTAT CGCTGCTGAT
    GTTGAAGTGG TGGAGATGCA TGACAGCGAT ATATCTGCAT ACACATATGA ACGCACACTC
    ATGATGGAGC AGAGGTCACA AATGCTGAGA CAGATGCGCC TTTCAAAAAC AGATCGGGAA
    AAGGAGGCTC AGTTGGTGAA GGACAGGAAT TCAATGCTGC GTTTAACCAG TGTGGGCTCT
    GATGATGATG AAGATACAGA GGCTGTCCCA GATAGAGTGC ACATGACTTG GACCAAGGAT
    AAGCACCATG CAGTTCGTGT TGCCCAAAGC AAACCAATGC CCAGCTTTCA GGACCTTCTC
    AACATACGAC CGGACCAGTC GAATGTTCGG CGTATGCACA CAGCTGTGAA ACTTAATGAA
    GTCATTGTTA ATAAATCCCA TGATGCCAAA TTGGTTTTGC TCAACATGCC AGGACCCCCT
    CGTAACCCTC AGGGTGATGA GAATTATATG GAGTTTCTAG AGGTGCTTAC TGAGGGCTTG
    GAACACGTGC TGTTAGTGAG AGGAGGTGGA ACAGAGGTGA TCACCATTTA CTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48038
    Clone ID Related Accession (Same CDS sequence) XM_012965533.3
    Accession Version XM_012965533.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012965533.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCACATT TTACAGTAAC TAAAGTACCT GACCCTCACT GTGCTACCGA GGAGGCGACC 
    TCCAGTCCTA CGAGCTGGGC TGACAGTGAG GAGGATTCAT GGGGAGCTGA TCGGAGCCAA
    ACATCAGTAA CAGAGGAGAA CAGTAAGCTG CTAGACATTG ACCGCCGGAA GAGTTCAGTG
    CTTTATCAAA ATCACACCGA TGATGAAGGG GACTCTTATG ACAGAAATCT GGCCCTGTTT
    GAGGAAGAAA TGGATACTCG ACCAAAAGTT TCATCCCTCC TTAACCGCTT GGCTAATTAC
    ACAAATCTGC CTCAGGGAGC AAAAGAACAT GAAGAGGAAA GTGAGGGCAA GAAGAAGGCT
    GCTAAAACCC CCCGAATGGG CACGATAATG GGGGTTTACC TCCCTTGTCT TCAGAACATT
    TTTGGGGTCA TTTTATTCCT TCGACTCCCG TGGGTGGTGG GCACAGCAGG AGTCTTGCAT
    GCCTTCTGCA TTGTATTCGC CTGCTGCTGC TGTACCATGC TGACGGCTAT TTCTATGAGC
    GCCATTGCAA CAAATGGGGT GGTGCCAGCT GGTGGTGCCT ATTTCATGAT TTCTCGAGCC
    CTTGGTCCAG AGTTTGGAGG TGCTGTAGGC CTGTGCTTCT ACCTTGGAAC AACCTTTGCA
    ACAGCTATGT ACATCCTGGG GGCTATTGAG ATATTCCTGG TGTACATTTC CCCCCAAGCA
    GTCATATTCC ATGGAGAGGG GGTGGCAGAG GAATCAGCAG CAATGTTGAA TAATATGCGT
    GTATATGGAA CTGGATTCCT GATTATTATG TCTATTATTG TGTTTGTTGG AGTGCGATAT
    GTAAACAAGT TGGCCTCTGT ATTCCTGACC TGTGTCATCA TGTCTATTCT GGCAATTTAT
    GCTGGGGCAC TAAAATCTGC ATTTTCTCCC CCGGACTTTC CGATTTGCTT GCTTGGAAAC
    CGGACTCTGT CCAAGCATCA TTTTACCGAA TGTGCCAAGA TAGTGGAGAG CGACAACATG
    ACCATTGTCA CAGATCTGTG GAATCTATTC TGCCACCCAT CGGATCAGCT GAATGGAACT
    TGTGATGAAT ATTTTGCCCA TAATAATGTC ACCTTAGTCC AGGGAATCCC AGGCTTAAAG
    AGCGGGATCA TCTCTGAAAA CCTGTGGAGC AATTACCTTC AAAAGGGAGA CATAATAGAA
    AAGGCTTCAT TGCCATCAAC TGAGTATTTG GCTGTGCAGT CTCAAGAGTA TGTTTTAGCT
    GACATTACGA CTTCTTTTAC CCTGTTGGTT GGAATATTTT TCCCATCTGT AACAGGAATT
    ATGGCTGGAT CAAATCGCTC TGGAGACCTC AAGGATGCCC AGAAATCGAT TCCAATTGGA
    ACTATTCTTG CAATTCTTAC AACCTCCTTA GTCTACCTCA GTAATGTCAT CCTGTTTGGA
    GCTTCTATAG ATGGAGTTGT GCTCAGAGAC AAGTTTGGGG ATGCAGTGAA AGGAACTCTT
    GTTGTTGGTG CGCTTTCATG GCCATCTCCT TGGGTCATTG TGATTGGATC TTTTTTCTCT
    ACTTGTGGAG CTGGACTTCA AAGTTTGACT GGAGCACCTC GCCTGCTCCA AGCCATTGCT
    AAGGATGGAA TCATTCCATT TCTGAGGGTA TTCGGACACG GAAAGAGTAA TGGGGAACCG
    ACATGGGCTC TTCTTCTAAC GGCAGTGATT GCTGAGCTGG GGATCCTTAT TGCTTCTCTG
    GACTTGGTGG CACCTATCCT ATCTATGTTC TTTCTAATGT GTTACCTTTT TGTAAACTTG
    GCATGTGGAT TACAGACCCT TCTCCATTCA CCCAACTGGC GCCCTCGCTT CCATTATTAT
    CACTGGACTT TATCTTTTCT TGGAATGGCA CTGTGTTTAG CCCTCATGTT TATCTCTTCC
    TGGTATTATG CCCTAATTTC CATGGTAATT GCTGGAATGA TCTACAAGTA CATTGAATAC
    CAAGGGGCCG AAAAAGAATG GGGAGATGGA ATCCGTGGAT TGTCCTTGAG TGCAGCACGT
    TTTGCTCTCC TGCGCCTGGA AGAGGGTCCA CCTCACACTA AGAACTGGAG ACCTCAGCTA
    CTTGTTTTGG TGAAATTGGA TTCAGATCTC CATGTCTCTC AGCCTCGCCT TCTTTCCTTC
    GCCTCCCAGC TAAAGGCTGG TAAAGGTCTG ACCATTGTGG GTTCAGTACT TCTGGGAGAC
    TACCTAGAGA ATCATGCAGA GGCCCAGGCA GCGGAGCAGG CACTGAAACA CTTAATGGAG
    CAAGAAAAAG TAAAAGGTTT TTGCCAAGTG GTTGTGGCTC AGAAGCTGAA AGAGGGTCTT
    TCCCACCTTA TACAGTCCTG TGGTCTTGGG GGAATGAGAC ACAACACTGT GATCATGAGC
    TGGCCAAGTA GCTGGAGACA GAGTGATGAC TCACGGGCTT GGAAATCCTT CATCACTACC
    ATTCGTGTAA CCACAGCTGC CCGCCAAGCA CTTCTGGTAG CAAAGAATGT ATCCCTGTTC
    CCTGGCTCCC GTGAAATATT AGCAGAAGGG CACATTGATG TTTGGTGGAT AGTACATGAT
    GGGGGCATGC TCATGCTTCT GCCCTTCCTC CTTAAACAAC ACAAGGTGTG GCGGAAATGT
    AAGATGCGTA TTTTCACTGT GGCTCAGATG GAAGACAATA GTATTCAGAT GAAAAAGGAC
    TTGGCTACCT TCCTTTATCA TTTACGTATC GCTGCTGATG TTGAAGTGGT GGAGATGCAT
    GACAGCGATA TATCTGCATA CACATATGAA CGCACACTCA TGATGGAGCA GAGGTCACAA
    ATGCTGAGAC AGATGCGCCT TTCAAAAACA GATCGGGAAA AGGAGGCTCA GTTGGTGAAG
    GACAGGAATT CAATGCTGCG TTTAACCAGT GTGGGCTCTG ATGATGATGA AGATACAGAG
    GCTGTCCCAG ATAGAGTGCA CATGACTTGG ACCAAGGATA AGCACCATGC AGTTCGTGTT
    GCCCAAAGCA AACCAATGCC CAGCTTTCAG GACCTTCTCA ACATACGACC GGACCAGTCG
    AATGTTCGGC GTATGCACAC AGCTGTGAAA CTTAATGAAG TCATTGTTAA TAAATCCCAT
    GATGCCAAAT TGGTTTTGCT CAACATGCCA GGACCCCCTC GTAACCCTCA GGGTGATGAG
    AATTATATGG AGTTTCTAGA GGTGCTTACT GAGGGCTTGG AACACGTGCT GTTAGTGAGA
    GGAGGTGGAA CAGAGGTGAT CACCATTTAC TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820987.1
    CDS3689..6964
    Translation

    Target ORF information:

    RefSeq Version XM_012965533.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965533.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCACATT TTACAGTAAC TAAAGTACCT GACCCTCACT GTGCTACCGA GGAGGCGACC 
    TCCAGTCCTA CGAGCTGGGC TGACAGTGAG GAGGATTCAT GGGGAGCTGA TCGGAGCCAA
    ACATCAGTAA CAGAGGAGAA CAGTAAGCTG CTAGACATTG ACCGCCGGAA GAGTTCAGTG
    CTTTATCAAA ATCACACCGA TGATGAAGGG GACTCTTATG ACAGAAATCT GGCCCTGTTT
    GAGGAAGAAA TGGATACTCG ACCAAAAGTT TCATCCCTCC TTAACCGCTT GGCTAATTAC
    ACAAATCTGC CTCAGGGAGC AAAAGAACAT GAAGAGGAAA GTGAGGGCAA GAAGAAGGCT
    GCTAAAACCC CCCGAATGGG CACGATAATG GGGGTTTACC TCCCTTGTCT TCAGAACATT
    TTTGGGGTCA TTTTATTCCT TCGACTCCCG TGGGTGGTGG GCACAGCAGG AGTCTTGCAT
    GCCTTCTGCA TTGTATTCGC CTGCTGCTGC TGTACCATGC TGACGGCTAT TTCTATGAGC
    GCCATTGCAA CAAATGGGGT GGTGCCAGCT GGTGGTGCCT ATTTCATGAT TTCTCGAGCC
    CTTGGTCCAG AGTTTGGAGG TGCTGTAGGC CTGTGCTTCT ACCTTGGAAC AACCTTTGCA
    ACAGCTATGT ACATCCTGGG GGCTATTGAG ATATTCCTGG TGTACATTTC CCCCCAAGCA
    GTCATATTCC ATGGAGAGGG GGTGGCAGAG GAATCAGCAG CAATGTTGAA TAATATGCGT
    GTATATGGAA CTGGATTCCT GATTATTATG TCTATTATTG TGTTTGTTGG AGTGCGATAT
    GTAAACAAGT TGGCCTCTGT ATTCCTGACC TGTGTCATCA TGTCTATTCT GGCAATTTAT
    GCTGGGGCAC TAAAATCTGC ATTTTCTCCC CCGGACTTTC CGATTTGCTT GCTTGGAAAC
    CGGACTCTGT CCAAGCATCA TTTTACCGAA TGTGCCAAGA TAGTGGAGAG CGACAACATG
    ACCATTGTCA CAGATCTGTG GAATCTATTC TGCCACCCAT CGGATCAGCT GAATGGAACT
    TGTGATGAAT ATTTTGCCCA TAATAATGTC ACCTTAGTCC AGGGAATCCC AGGCTTAAAG
    AGCGGGATCA TCTCTGAAAA CCTGTGGAGC AATTACCTTC AAAAGGGAGA CATAATAGAA
    AAGGCTTCAT TGCCATCAAC TGAGTATTTG GCTGTGCAGT CTCAAGAGTA TGTTTTAGCT
    GACATTACGA CTTCTTTTAC CCTGTTGGTT GGAATATTTT TCCCATCTGT AACAGGAATT
    ATGGCTGGAT CAAATCGCTC TGGAGACCTC AAGGATGCCC AGAAATCGAT TCCAATTGGA
    ACTATTCTTG CAATTCTTAC AACCTCCTTA GTCTACCTCA GTAATGTCAT CCTGTTTGGA
    GCTTCTATAG ATGGAGTTGT GCTCAGAGAC AAGTTTGGGG ATGCAGTGAA AGGAACTCTT
    GTTGTTGGTG CGCTTTCATG GCCATCTCCT TGGGTCATTG TGATTGGATC TTTTTTCTCT
    ACTTGTGGAG CTGGACTTCA AAGTTTGACT GGAGCACCTC GCCTGCTCCA AGCCATTGCT
    AAGGATGGAA TCATTCCATT TCTGAGGGTA TTCGGACACG GAAAGAGTAA TGGGGAACCG
    ACATGGGCTC TTCTTCTAAC GGCAGTGATT GCTGAGCTGG GGATCCTTAT TGCTTCTCTG
    GACTTGGTGG CACCTATCCT ATCTATGTTC TTTCTAATGT GTTACCTTTT TGTAAACTTG
    GCATGTGGAT TACAGACCCT TCTCCATTCA CCCAACTGGC GCCCTCGCTT CCATTATTAT
    CACTGGACTT TATCTTTTCT TGGAATGGCA CTGTGTTTAG CCCTCATGTT TATCTCTTCC
    TGGTATTATG CCCTAATTTC CATGGTAATT GCTGGAATGA TCTACAAGTA CATTGAATAC
    CAAGGGGCCG AAAAAGAATG GGGAGATGGA ATCCGTGGAT TGTCCTTGAG TGCAGCACGT
    TTTGCTCTCC TGCGCCTGGA AGAGGGTCCA CCTCACACTA AGAACTGGAG ACCTCAGCTA
    CTTGTTTTGG TGAAATTGGA TTCAGATCTC CATGTCTCTC AGCCTCGCCT TCTTTCCTTC
    GCCTCCCAGC TAAAGGCTGG TAAAGGTCTG ACCATTGTGG GTTCAGTACT TCTGGGAGAC
    TACCTAGAGA ATCATGCAGA GGCCCAGGCA GCGGAGCAGG CACTGAAACA CTTAATGGAG
    CAAGAAAAAG TAAAAGGTTT TTGCCAAGTG GTTGTGGCTC AGAAGCTGAA AGAGGGTCTT
    TCCCACCTTA TACAGTCCTG TGGTCTTGGG GGAATGAGAC ACAACACTGT GATCATGAGC
    TGGCCAAGTA GCTGGAGACA GAGTGATGAC TCACGGGCTT GGAAATCCTT CATCACTACC
    ATTCGTGTAA CCACAGCTGC CCGCCAAGCA CTTCTGGTAG CAAAGAATGT ATCCCTGTTC
    CCTGGCTCCC GTGAAATATT AGCAGAAGGG CACATTGATG TTTGGTGGAT AGTACATGAT
    GGGGGCATGC TCATGCTTCT GCCCTTCCTC CTTAAACAAC ACAAGGTGTG GCGGAAATGT
    AAGATGCGTA TTTTCACTGT GGCTCAGATG GAAGACAATA GTATTCAGAT GAAAAAGGAC
    TTGGCTACCT TCCTTTATCA TTTACGTATC GCTGCTGATG TTGAAGTGGT GGAGATGCAT
    GACAGCGATA TATCTGCATA CACATATGAA CGCACACTCA TGATGGAGCA GAGGTCACAA
    ATGCTGAGAC AGATGCGCCT TTCAAAAACA GATCGGGAAA AGGAGGCTCA GTTGGTGAAG
    GACAGGAATT CAATGCTGCG TTTAACCAGT GTGGGCTCTG ATGATGATGA AGATACAGAG
    GCTGTCCCAG ATAGAGTGCA CATGACTTGG ACCAAGGATA AGCACCATGC AGTTCGTGTT
    GCCCAAAGCA AACCAATGCC CAGCTTTCAG GACCTTCTCA ACATACGACC GGACCAGTCG
    AATGTTCGGC GTATGCACAC AGCTGTGAAA CTTAATGAAG TCATTGTTAA TAAATCCCAT
    GATGCCAAAT TGGTTTTGCT CAACATGCCA GGACCCCCTC GTAACCCTCA GGGTGATGAG
    AATTATATGG AGTTTCTAGA GGTGCTTACT GAGGGCTTGG AACACGTGCT GTTAGTGAGA
    GGAGGTGGAA CAGAGGTGAT CACCATTTAC TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48039
    Clone ID Related Accession (Same CDS sequence) XM_031901800.1
    Accession Version XM_031901800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3231bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCACATT TTACAGTAAC TAAAGTACCT GACCCTCACT GTGCTACCGA GGAGGCGACC 
    TCCAGTCCTA CGAGCTGGGC TGACAGTGAG GAGGATTCAT GGGGAGCTGA CATTGACCGC
    CGGAAGAGTT CAGTGCTTTA TCAAAATCAC ACCGATGATG AAGGGGACTC TTATGACAGA
    AATCTGGCCC TGTTTGAGGA AGAAATGGAT ACTCGACCAA AAGTTTCATC CCTCCTTAAC
    CGCTTGGCTA ATTACACAAA TCTGCCTCAG GGAGCAAAAG AACATGAAGA GGAAAGTGAG
    GGCAAGAAGA AGGCTGCTAA AACCCCCCGA ATGGGCACGA TAATGGGGGT TTACCTCCCT
    TGTCTTCAGA ACATTTTTGG GGTCATTTTA TTCCTTCGAC TCCCGTGGGT GGTGGGCACA
    GCAGGAGTCT TGCATGCCTT CTGCATTGTA TTCGCCTGCT GCTGCTGTAC CATGCTGACG
    GCTATTTCTA TGAGCGCCAT TGCAACAAAT GGGGTGGTGC CAGCTGGTGG TGCCTATTTC
    ATGATTTCTC GAGCCCTTGG TCCAGAGTTT GGAGGTGCTG TAGGCCTGTG CTTCTACCTT
    GGAACAACCT TTGCAACAGC TATGTACATC CTGGGGGCTA TTGAGATATT CCTGGTGTAC
    ATTTCCCCCC AAGCAGTCAT ATTCCATGGA GAGGGGGTGG CAGAGGAATC AGCAGCAATG
    TTGAATAATA TGCGTGTATA TGGAACTGGA TTCCTGATTA TTATGTCTAT TATTGTGTTT
    GTTGGAGTGC GATATGTAAA CAAGTTGGCC TCTGTATTCC TGACCTGTGT CATCATGTCT
    ATTCTGGCAA TTTATGCTGG GGCACTAAAA TCTGCATTTT CTCCCCCGGA CTTTCCGATT
    TGCTTGCTTG GAAACCGGAC TCTGTCCAAG CATCATTTTA CCGAATGTGC CAAGATAGTG
    GAGAGCGACA ACATGACCAT TGTCACAGAT CTGTGGAATC TATTCTGCCA CCCATCGGAT
    CAGCTGAATG GAACTTGTGA TGAATATTTT GCCCATAATA ATGTCACCTT AGTCCAGGGA
    ATCCCAGGCT TAAAGAGCGG GATCATCTCT GAAAACCTGT GGAGCAATTA CCTTCAAAAG
    GGAGACATAA TAGAAAAGGC TTCATTGCCA TCAACTGAGT ATTTGGCTGT GCAGTCTCAA
    GAGTATGTTT TAGCTGACAT TACGACTTCT TTTACCCTGT TGGTTGGAAT ATTTTTCCCA
    TCTGTAACAG GAATTATGGC TGGATCAAAT CGCTCTGGAG ACCTCAAGGA TGCCCAGAAA
    TCGATTCCAA TTGGAACTAT TCTTGCAATT CTTACAACCT CCTTAGTCTA CCTCAGTAAT
    GTCATCCTGT TTGGAGCTTC TATAGATGGA GTTGTGCTCA GAGACAAGTT TGGGGATGCA
    GTGAAAGGAA CTCTTGTTGT TGGTGCGCTT TCATGGCCAT CTCCTTGGGT CATTGTGATT
    GGATCTTTTT TCTCTACTTG TGGAGCTGGA CTTCAAAGTT TGACTGGAGC ACCTCGCCTG
    CTCCAAGCCA TTGCTAAGGA TGGAATCATT CCATTTCTGA GGGTATTCGG ACACGGAAAG
    AGTAATGGGG AACCGACATG GGCTCTTCTT CTAACGGCAG TGATTGCTGA GCTGGGGATC
    CTTATTGCTT CTCTGGACTT GGTGGCACCT ATCCTATCTA TGTTCTTTCT AATGTGTTAC
    CTTTTTGTAA ACTTGGCATG TGGATTACAG ACCCTTCTCC ATTCACCCAA CTGGCGCCCT
    CGCTTCCATT ATTATCACTG GACTTTATCT TTTCTTGGAA TGGCACTGTG TTTAGCCCTC
    ATGTTTATCT CTTCCTGGTA TTATGCCCTA ATTTCCATGG TAATTGCTGG AATGATCTAC
    AAGTACATTG AATACCAAGG GGCCGAAAAA GAATGGGGAG ATGGAATCCG TGGATTGTCC
    TTGAGTGCAG CACGTTTTGC TCTCCTGCGC CTGGAAGAGG GTCCACCTCA CACTAAGAAC
    TGGAGACCTC AGCTACTTGT TTTGGTGAAA TTGGATTCAG ATCTCCATGT CTCTCAGCCT
    CGCCTTCTTT CCTTCGCCTC CCAGCTAAAG GCTGGTAAAG GTCTGACCAT TGTGGGTTCA
    GTACTTCTGG GAGACTACCT AGAGAATCAT GCAGAGGCCC AGGCAGCGGA GCAGGCACTG
    AAACACTTAA TGGAGCAAGA AAAAGTAAAA GGTTTTTGCC AAGTGGTTGT GGCTCAGAAG
    CTGAAAGAGG GTCTTTCCCA CCTTATACAG TCCTGTGGTC TTGGGGGAAT GAGACACAAC
    ACTGTGATCA TGAGCTGGCC AAGTAGCTGG AGACAGAGTG ATGACTCACG GGCTTGGAAA
    TCCTTCATCA CTACCATTCG TGTAACCACA GCTGCCCGCC AAGCACTTCT GGTAGCAAAG
    AATGTATCCC TGTTCCCTGG CTCCCGTGAA ATATTAGCAG AAGGGCACAT TGATGTTTGG
    TGGATAGTAC ATGATGGGGG CATGCTCATG CTTCTGCCCT TCCTCCTTAA ACAACACAAG
    GTGTGGCGGA AATGTAAGAT GCGTATTTTC ACTGTGGCTC AGATGGAAGA CAATAGTATT
    CAGATGAAAA AGGACTTGGC TACCTTCCTT TATCATTTAC GTATCGCTGC TGATGTTGAA
    GTGGTGGAGA TGCATGACAG CGATATATCT GCATACACAT ATGAACGCAC ACTCATGATG
    GAGCAGAGGT CACAAATGCT GAGACAGATG CGCCTTTCAA AAACAGATCG GGAAAAGGAG
    GCTCAGTTGG TGAAGGACAG GAATTCAATG CTGCGTTTAA CCAGTGTGGG CTCTGATGAT
    GATGAAGATA CAGAGGCTGT CCCAGATAGA GTGCACATGA CTTGGACCAA GGATAAGCAC
    CATGCAGTTC GTGTTGCCCA AAGCAAACCA ATGCCCAGCT TTCAGGACCT TCTCAACATA
    CGACCGGACC AGTCGAATGT TCGGCGTATG CACACAGCTG TGAAACTTAA TGAAGTCATT
    GTTAATAAAT CCCATGATGC CAAATTGGTT TTGCTCAACA TGCCAGGACC CCCTCGTAAC
    CCTCAGGGTG ATGAGAATTA TATGGAGTTT CTAGAGGTGC TTACTGAGGG CTTGGAACAC
    GTGCTGTTAG TGAGAGGAGG TGGAACAGAG GTGATCACCA TTTACTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031757660.1
    CDS3689..6919
    Translation

    Target ORF information:

    RefSeq Version XM_031901800.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031901800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCCACATT TTACAGTAAC TAAAGTACCT GACCCTCACT GTGCTACCGA GGAGGCGACC 
    TCCAGTCCTA CGAGCTGGGC TGACAGTGAG GAGGATTCAT GGGGAGCTGA CATTGACCGC
    CGGAAGAGTT CAGTGCTTTA TCAAAATCAC ACCGATGATG AAGGGGACTC TTATGACAGA
    AATCTGGCCC TGTTTGAGGA AGAAATGGAT ACTCGACCAA AAGTTTCATC CCTCCTTAAC
    CGCTTGGCTA ATTACACAAA TCTGCCTCAG GGAGCAAAAG AACATGAAGA GGAAAGTGAG
    GGCAAGAAGA AGGCTGCTAA AACCCCCCGA ATGGGCACGA TAATGGGGGT TTACCTCCCT
    TGTCTTCAGA ACATTTTTGG GGTCATTTTA TTCCTTCGAC TCCCGTGGGT GGTGGGCACA
    GCAGGAGTCT TGCATGCCTT CTGCATTGTA TTCGCCTGCT GCTGCTGTAC CATGCTGACG
    GCTATTTCTA TGAGCGCCAT TGCAACAAAT GGGGTGGTGC CAGCTGGTGG TGCCTATTTC
    ATGATTTCTC GAGCCCTTGG TCCAGAGTTT GGAGGTGCTG TAGGCCTGTG CTTCTACCTT
    GGAACAACCT TTGCAACAGC TATGTACATC CTGGGGGCTA TTGAGATATT CCTGGTGTAC
    ATTTCCCCCC AAGCAGTCAT ATTCCATGGA GAGGGGGTGG CAGAGGAATC AGCAGCAATG
    TTGAATAATA TGCGTGTATA TGGAACTGGA TTCCTGATTA TTATGTCTAT TATTGTGTTT
    GTTGGAGTGC GATATGTAAA CAAGTTGGCC TCTGTATTCC TGACCTGTGT CATCATGTCT
    ATTCTGGCAA TTTATGCTGG GGCACTAAAA TCTGCATTTT CTCCCCCGGA CTTTCCGATT
    TGCTTGCTTG GAAACCGGAC TCTGTCCAAG CATCATTTTA CCGAATGTGC CAAGATAGTG
    GAGAGCGACA ACATGACCAT TGTCACAGAT CTGTGGAATC TATTCTGCCA CCCATCGGAT
    CAGCTGAATG GAACTTGTGA TGAATATTTT GCCCATAATA ATGTCACCTT AGTCCAGGGA
    ATCCCAGGCT TAAAGAGCGG GATCATCTCT GAAAACCTGT GGAGCAATTA CCTTCAAAAG
    GGAGACATAA TAGAAAAGGC TTCATTGCCA TCAACTGAGT ATTTGGCTGT GCAGTCTCAA
    GAGTATGTTT TAGCTGACAT TACGACTTCT TTTACCCTGT TGGTTGGAAT ATTTTTCCCA
    TCTGTAACAG GAATTATGGC TGGATCAAAT CGCTCTGGAG ACCTCAAGGA TGCCCAGAAA
    TCGATTCCAA TTGGAACTAT TCTTGCAATT CTTACAACCT CCTTAGTCTA CCTCAGTAAT
    GTCATCCTGT TTGGAGCTTC TATAGATGGA GTTGTGCTCA GAGACAAGTT TGGGGATGCA
    GTGAAAGGAA CTCTTGTTGT TGGTGCGCTT TCATGGCCAT CTCCTTGGGT CATTGTGATT
    GGATCTTTTT TCTCTACTTG TGGAGCTGGA CTTCAAAGTT TGACTGGAGC ACCTCGCCTG
    CTCCAAGCCA TTGCTAAGGA TGGAATCATT CCATTTCTGA GGGTATTCGG ACACGGAAAG
    AGTAATGGGG AACCGACATG GGCTCTTCTT CTAACGGCAG TGATTGCTGA GCTGGGGATC
    CTTATTGCTT CTCTGGACTT GGTGGCACCT ATCCTATCTA TGTTCTTTCT AATGTGTTAC
    CTTTTTGTAA ACTTGGCATG TGGATTACAG ACCCTTCTCC ATTCACCCAA CTGGCGCCCT
    CGCTTCCATT ATTATCACTG GACTTTATCT TTTCTTGGAA TGGCACTGTG TTTAGCCCTC
    ATGTTTATCT CTTCCTGGTA TTATGCCCTA ATTTCCATGG TAATTGCTGG AATGATCTAC
    AAGTACATTG AATACCAAGG GGCCGAAAAA GAATGGGGAG ATGGAATCCG TGGATTGTCC
    TTGAGTGCAG CACGTTTTGC TCTCCTGCGC CTGGAAGAGG GTCCACCTCA CACTAAGAAC
    TGGAGACCTC AGCTACTTGT TTTGGTGAAA TTGGATTCAG ATCTCCATGT CTCTCAGCCT
    CGCCTTCTTT CCTTCGCCTC CCAGCTAAAG GCTGGTAAAG GTCTGACCAT TGTGGGTTCA
    GTACTTCTGG GAGACTACCT AGAGAATCAT GCAGAGGCCC AGGCAGCGGA GCAGGCACTG
    AAACACTTAA TGGAGCAAGA AAAAGTAAAA GGTTTTTGCC AAGTGGTTGT GGCTCAGAAG
    CTGAAAGAGG GTCTTTCCCA CCTTATACAG TCCTGTGGTC TTGGGGGAAT GAGACACAAC
    ACTGTGATCA TGAGCTGGCC AAGTAGCTGG AGACAGAGTG ATGACTCACG GGCTTGGAAA
    TCCTTCATCA CTACCATTCG TGTAACCACA GCTGCCCGCC AAGCACTTCT GGTAGCAAAG
    AATGTATCCC TGTTCCCTGG CTCCCGTGAA ATATTAGCAG AAGGGCACAT TGATGTTTGG
    TGGATAGTAC ATGATGGGGG CATGCTCATG CTTCTGCCCT TCCTCCTTAA ACAACACAAG
    GTGTGGCGGA AATGTAAGAT GCGTATTTTC ACTGTGGCTC AGATGGAAGA CAATAGTATT
    CAGATGAAAA AGGACTTGGC TACCTTCCTT TATCATTTAC GTATCGCTGC TGATGTTGAA
    GTGGTGGAGA TGCATGACAG CGATATATCT GCATACACAT ATGAACGCAC ACTCATGATG
    GAGCAGAGGT CACAAATGCT GAGACAGATG CGCCTTTCAA AAACAGATCG GGAAAAGGAG
    GCTCAGTTGG TGAAGGACAG GAATTCAATG CTGCGTTTAA CCAGTGTGGG CTCTGATGAT
    GATGAAGATA CAGAGGCTGT CCCAGATAGA GTGCACATGA CTTGGACCAA GGATAAGCAC
    CATGCAGTTC GTGTTGCCCA AAGCAAACCA ATGCCCAGCT TTCAGGACCT TCTCAACATA
    CGACCGGACC AGTCGAATGT TCGGCGTATG CACACAGCTG TGAAACTTAA TGAAGTCATT
    GTTAATAAAT CCCATGATGC CAAATTGGTT TTGCTCAACA TGCCAGGACC CCCTCGTAAC
    CCTCAGGGTG ATGAGAATTA TATGGAGTTT CTAGAGGTGC TTACTGAGGG CTTGGAACAC
    GTGCTGTTAG TGAGAGGAGG TGGAACAGAG GTGATCACCA TTTACTCATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa24444
    Clone ID Related Accession (Same CDS sequence) NM_001079108.1
    Accession Version NM_001079108.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC122930.1. ##Evidence-Data-START## Transcript exon combination :: BC122930.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028291, SAMD00028292 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCATCGG TACGGTTCAC CGTTACACCC ACCAAAATGG ACGAAATGGG GGCAATCTCT 
    GACACCAGCC CAGATCTCAG TTCATCCTCC CGTGTGCGGT TTAGCTCCAG AGAGAGTGTC
    AGAGATGCCA GCCGCAGTGA GGCTTACAGT GACACCTCAC TGGCAACCAC AGGAGTGGAC
    CAAGCTAGTG AAAGGACCTC CAACCCTATG GACAGTGGAG AAGATCGGAG CCAAACATCA
    GTAACAGAGG AGAACAGTAA GCTGCTAGAC ATTGACCGCC GGAAGAGTTC AGTGCTTTAT
    CAAAATCACA CCGATGATGA AGGGGACTCT TATGACAGAA ATCTGGCCCT GTTTGAGGAA
    GAAATGGATA CTCGACCAAA AGTTTCATCC CTCCTTAACC GCTTGGCTAA TTACACAAAT
    CTGCCTCAGG GAGCAAAAGA ACATGAAGAG GAAAGTGAGG GCAAGAAGAA GGCTGCTAAA
    ACCCCCCGAA TGGGCACGAT AATGGGGGTT TACCTCCCTT GTCTTCAGAA CATTTTTGGG
    GTCATTTTAT TCCTTCGACT CCCGTGGGTG GTGGGCACAG CAGGAGTCTT GCATGCCTTC
    TGCATTGTAT TCGCCTGCTG CTGCTGTACC ATGCTGACGG CTATTTCTAT GAGCGCCATT
    GCAACAAATG GGGTGGTGCC AGCTGGTGGT GCCTATTTCA TGATTTCTCG AGCCCTTGGT
    CCAGAGTTTG GAGGTGCTGT AGGCCTGTGC TTCTACCTTG GAACAACCTT TGCAACAGCT
    ATGTACATCC TGGGGGCTAT TGAGATATTC CTGGTGTACA TTTCCCCCCA AGCAGTCATA
    TTCCATGGAG AGGGGGTGGC AGAGGAATCA GCAGCAATGT TGAATAATAT GCGTGTATAT
    GGAACTGGAT TCCTGATTAT TATGTCTATT ATTGTGTTTG TTGGAGTGCG ATATGTAAAC
    AAGTTGGCCT CTGTATTCCT GACCTGTGTC ATCATGTCTA TTCTGGCAAT TTATGCTGGG
    GCACTAAAAT CTGCATTTTC TCCCCCGGAC TTTCCGATTT GCTTGCTTGG AAACCGGACT
    CTGTCCAAGC ATCATTTTAC CGAATGTGCC AAGATAGTGG AGAGCGACAA CATGACCATT
    GTCACAGATC TGTGGAATCT ATTCTGCCAC CCATCGGATC AGCTGAATGG AACTTGTGAT
    GAATATTTTG CCCATAATAA TGTCACCTTA GTCCAGGGAA TCCCAGGCTT AAAGAGCGGG
    ATCATCTCTG AAAACCTGTG GAGCAATTAC CTTCAAAAGG GAGACATAAT AGAAAAGGCT
    TCATTGCCAT CAACTGAGTA TTTGGCTGTG CAGTCTCAAG AGTATGTTTT AGCTGACATT
    ACGACTTCTT TTACCCTGTT GGTTGGAATA TTTTTCCCAT CTGTAACAGG AATTATGGCT
    GGATCAAATC GCTCTGGAGA CCTCAAGGAT GCCCAGAAAT CGATTCCAAT TGGAACTATT
    CTTGCAATTC TTACAACCTC CTTAGTCTAC CTCAGTAATG TCATCCTGTT TGGAGCTTCT
    ATAGATGGAG TTGTGCTCAG AGACAAGTTT GGGGATGCAG TGAAAGGAAC TCTTGTTGTT
    GGTGCGCTTT CATGGCCATC TCCTTGGGTC ATTGTGATTG GATCTTTTTT CTCTACTTGT
    GGAGCTGGAC TTCAAAGTTT GACTGGAGCA CCTCGCCTGC TCCAAGCCAT TGCTAAGGAT
    GGAATCATTC CATTTCTGAG GGTATTCGGA CACGGAAAGA GTAATGGGGA ACCGACATGG
    GCTCTTCTTC TAACGGCAGT GATTGCTGAG CTGGGGATCC TTATTGCTTC TCTGGACTTG
    GTGGCACCTA TCCTATCTAT GTTCTTTCTA ATGTGTTACC TTTTTGTAAA CTTGGCATGT
    GGATTACAGA CCCTTCTCCA TTCACCCAAC TGGCGCCCTC GCTTCCATTA TTATCACTGG
    ACTTTATCTT TTCTTGGAAT GGCACTGTGT TTAGCCCTCA TGTTTATCTC TTCCTGGTAT
    TATGCCCTAA TTTCCATGGT AATTGCTGGA ATGATCTACA AGTACATTGA ATACCAAGGG
    GCCGAAAAAG AATGGGGAGA TGGAATCCGT GGATTGTCCT TGAGTGCAGC ACGTTTTGCT
    CTCCTGCGCC TGGAAGAGGG TCCACCTCAC ACTAAGAACT GGAGACCTCA GCTACTTGTT
    TTGGTGAAAT TGGATTCAGA TCTCCATGTC TCTCAGCCTC GCCTTCTTTC CTTCGCCTCC
    CAGCTAAAGG CTGGTAAAGG TCTGACCATT GTGGGTTCAG TACTTCTGGG AGACTACCTA
    GAGAATCATG CAGAGGCCCA GGCAGCGGAG CAGGCACTGA AACACTTAAT GGAGCAAGAA
    AAAGTAAAAG GTTTTTGCCA AGTGGTTGTG GCTCAGAAGC TGAAAGAGGG TCTTTCCCAC
    CTTATACAGT CCTGTGGTCT TGGGGGAATG AGACACAACA CTGTGATCAT GAGCTGGCCA
    AGTAGCTGGA GACAGAGTGA TGACTCACGG GCTTGGAAAT CCTTCATCAC TACCATTCGT
    GTAACCACAG CTGCCCGCCA AGCACTTCTG GTAGCAAAGA ATGTATCCCT GTTCCCTGGC
    TCCCGTGAAA TATTAGCAGA AGGGCACATT GATGTTTGGT GGATAGTACA TGATGGGGGC
    ATGCTCATGC TTCTGCCCTT CCTCCTTAAA CAACACAAGG TGTGGCGGAA ATGTAAGATG
    CGTATTTTCA CTGTGGCTCA GATGGAAGAC AATAGTATTC AGATGAAAAA GGACTTGGCT
    ACCTTCCTTT ATCATTTACG TATCGCTGCT GATGTTGAAG TGGTGGAGAT GCATGACAGC
    GATATATCTG CATACACATA TGAACGCACA CTCATGATGG AGCAGAGGTC ACAAATGCTG
    AGACAGATGC GCCTTTCAAA AACAGATCGG GAAAAGGAGG CTCAGTTGGT GAAGGACAGG
    AATTCAATGC TGCGTTTAAC CAGTGTGGGC TCTGATGATG ATGAAGATAC AGAGGCTGTC
    CCAGATAGAG TGCACATGAC TTGGACCAAG GATAAGCACC ATGCAGTTCG TGTTGCCCAA
    AGCAAACCAA TGCCCAGCTT TCAGGACCTT CTCAACATAC GACCGGACCA GTCGAATGTT
    CGGCGTATGC ACACAGCTGT GAAACTTAAT GAAGTCATTG TTAATAAATC CCATGATGCC
    AAATTGGTTT TGCTCAACAT GCCAGGACCC CCTCGTAACC CTCAGGGTGA TGAGAATTAT
    ATGGAGTTTC TAGAGGTGCT TACTGAGGGC TTGGAACACG TGCTGTTAGT GAGAGGAGGT
    GGAACAGAGG TGATCACCAT TTACTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001072576.1
    CDS314..3703
    Translation

    Target ORF information:

    RefSeq Version NM_001079108.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 member 6 (slc12a6), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001079108.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCATCGG TACGGTTCAC CGTTACACCC ACCAAAATGG ACGAAATGGG GGCAATCTCT 
    GACACCAGCC CAGATCTCAG TTCATCCTCC CGTGTGCGGT TTAGCTCCAG AGAGAGTGTC
    AGAGATGCCA GCCGCAGTGA GGCTTACAGT GACACCTCAC TGGCAACCAC AGGAGTGGAC
    CAAGCTAGTG AAAGGACCTC CAACCCTATG GACAGTGGAG AAGATCGGAG CCAAACATCA
    GTAACAGAGG AGAACAGTAA GCTGCTAGAC ATTGACCGCC GGAAGAGTTC AGTGCTTTAT
    CAAAATCACA CCGATGATGA AGGGGACTCT TATGACAGAA ATCTGGCCCT GTTTGAGGAA
    GAAATGGATA CTCGACCAAA AGTTTCATCC CTCCTTAACC GCTTGGCTAA TTACACAAAT
    CTGCCTCAGG GAGCAAAAGA ACATGAAGAG GAAAGTGAGG GCAAGAAGAA GGCTGCTAAA
    ACCCCCCGAA TGGGCACGAT AATGGGGGTT TACCTCCCTT GTCTTCAGAA CATTTTTGGG
    GTCATTTTAT TCCTTCGACT CCCGTGGGTG GTGGGCACAG CAGGAGTCTT GCATGCCTTC
    TGCATTGTAT TCGCCTGCTG CTGCTGTACC ATGCTGACGG CTATTTCTAT GAGCGCCATT
    GCAACAAATG GGGTGGTGCC AGCTGGTGGT GCCTATTTCA TGATTTCTCG AGCCCTTGGT
    CCAGAGTTTG GAGGTGCTGT AGGCCTGTGC TTCTACCTTG GAACAACCTT TGCAACAGCT
    ATGTACATCC TGGGGGCTAT TGAGATATTC CTGGTGTACA TTTCCCCCCA AGCAGTCATA
    TTCCATGGAG AGGGGGTGGC AGAGGAATCA GCAGCAATGT TGAATAATAT GCGTGTATAT
    GGAACTGGAT TCCTGATTAT TATGTCTATT ATTGTGTTTG TTGGAGTGCG ATATGTAAAC
    AAGTTGGCCT CTGTATTCCT GACCTGTGTC ATCATGTCTA TTCTGGCAAT TTATGCTGGG
    GCACTAAAAT CTGCATTTTC TCCCCCGGAC TTTCCGATTT GCTTGCTTGG AAACCGGACT
    CTGTCCAAGC ATCATTTTAC CGAATGTGCC AAGATAGTGG AGAGCGACAA CATGACCATT
    GTCACAGATC TGTGGAATCT ATTCTGCCAC CCATCGGATC AGCTGAATGG AACTTGTGAT
    GAATATTTTG CCCATAATAA TGTCACCTTA GTCCAGGGAA TCCCAGGCTT AAAGAGCGGG
    ATCATCTCTG AAAACCTGTG GAGCAATTAC CTTCAAAAGG GAGACATAAT AGAAAAGGCT
    TCATTGCCAT CAACTGAGTA TTTGGCTGTG CAGTCTCAAG AGTATGTTTT AGCTGACATT
    ACGACTTCTT TTACCCTGTT GGTTGGAATA TTTTTCCCAT CTGTAACAGG AATTATGGCT
    GGATCAAATC GCTCTGGAGA CCTCAAGGAT GCCCAGAAAT CGATTCCAAT TGGAACTATT
    CTTGCAATTC TTACAACCTC CTTAGTCTAC CTCAGTAATG TCATCCTGTT TGGAGCTTCT
    ATAGATGGAG TTGTGCTCAG AGACAAGTTT GGGGATGCAG TGAAAGGAAC TCTTGTTGTT
    GGTGCGCTTT CATGGCCATC TCCTTGGGTC ATTGTGATTG GATCTTTTTT CTCTACTTGT
    GGAGCTGGAC TTCAAAGTTT GACTGGAGCA CCTCGCCTGC TCCAAGCCAT TGCTAAGGAT
    GGAATCATTC CATTTCTGAG GGTATTCGGA CACGGAAAGA GTAATGGGGA ACCGACATGG
    GCTCTTCTTC TAACGGCAGT GATTGCTGAG CTGGGGATCC TTATTGCTTC TCTGGACTTG
    GTGGCACCTA TCCTATCTAT GTTCTTTCTA ATGTGTTACC TTTTTGTAAA CTTGGCATGT
    GGATTACAGA CCCTTCTCCA TTCACCCAAC TGGCGCCCTC GCTTCCATTA TTATCACTGG
    ACTTTATCTT TTCTTGGAAT GGCACTGTGT TTAGCCCTCA TGTTTATCTC TTCCTGGTAT
    TATGCCCTAA TTTCCATGGT AATTGCTGGA ATGATCTACA AGTACATTGA ATACCAAGGG
    GCCGAAAAAG AATGGGGAGA TGGAATCCGT GGATTGTCCT TGAGTGCAGC ACGTTTTGCT
    CTCCTGCGCC TGGAAGAGGG TCCACCTCAC ACTAAGAACT GGAGACCTCA GCTACTTGTT
    TTGGTGAAAT TGGATTCAGA TCTCCATGTC TCTCAGCCTC GCCTTCTTTC CTTCGCCTCC
    CAGCTAAAGG CTGGTAAAGG TCTGACCATT GTGGGTTCAG TACTTCTGGG AGACTACCTA
    GAGAATCATG CAGAGGCCCA GGCAGCGGAG CAGGCACTGA AACACTTAAT GGAGCAAGAA
    AAAGTAAAAG GTTTTTGCCA AGTGGTTGTG GCTCAGAAGC TGAAAGAGGG TCTTTCCCAC
    CTTATACAGT CCTGTGGTCT TGGGGGAATG AGACACAACA CTGTGATCAT GAGCTGGCCA
    AGTAGCTGGA GACAGAGTGA TGACTCACGG GCTTGGAAAT CCTTCATCAC TACCATTCGT
    GTAACCACAG CTGCCCGCCA AGCACTTCTG GTAGCAAAGA ATGTATCCCT GTTCCCTGGC
    TCCCGTGAAA TATTAGCAGA AGGGCACATT GATGTTTGGT GGATAGTACA TGATGGGGGC
    ATGCTCATGC TTCTGCCCTT CCTCCTTAAA CAACACAAGG TGTGGCGGAA ATGTAAGATG
    CGTATTTTCA CTGTGGCTCA GATGGAAGAC AATAGTATTC AGATGAAAAA GGACTTGGCT
    ACCTTCCTTT ATCATTTACG TATCGCTGCT GATGTTGAAG TGGTGGAGAT GCATGACAGC
    GATATATCTG CATACACATA TGAACGCACA CTCATGATGG AGCAGAGGTC ACAAATGCTG
    AGACAGATGC GCCTTTCAAA AACAGATCGG GAAAAGGAGG CTCAGTTGGT GAAGGACAGG
    AATTCAATGC TGCGTTTAAC CAGTGTGGGC TCTGATGATG ATGAAGATAC AGAGGCTGTC
    CCAGATAGAG TGCACATGAC TTGGACCAAG GATAAGCACC ATGCAGTTCG TGTTGCCCAA
    AGCAAACCAA TGCCCAGCTT TCAGGACCTT CTCAACATAC GACCGGACCA GTCGAATGTT
    CGGCGTATGC ACACAGCTGT GAAACTTAAT GAAGTCATTG TTAATAAATC CCATGATGCC
    AAATTGGTTT TGCTCAACAT GCCAGGACCC CCTCGTAACC CTCAGGGTGA TGAGAATTAT
    ATGGAGTTTC TAGAGGTGCT TACTGAGGGC TTGGAACACG TGCTGTTAGT GAGAGGAGGT
    GGAACAGAGG TGATCACCAT TTACTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29362
    Clone ID Related Accession (Same CDS sequence) XM_018094514.1 , XM_012965517.2 , XM_012965504.2 , XM_012965540.2 , XM_012965505.2 , XM_012965500.2 , XM_012965513.2 , XM_012965521.1
    Accession Version XM_018094514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950003.1
    CDS488..3889
    Translation

    Target ORF information:

    RefSeq Version XM_018094514.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29362
    Clone ID Related Accession (Same CDS sequence) XM_018094514.1 , XM_012965517.2 , XM_012965504.2 , XM_012965540.2 , XM_012965505.2 , XM_012965500.2 , XM_012965513.2 , XM_012965521.1
    Accession Version XM_012965517.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965517.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820971.1
    CDS482..3883
    Translation

    Target ORF information:

    RefSeq Version XM_012965517.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965517.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29362
    Clone ID Related Accession (Same CDS sequence) XM_018094514.1 , XM_012965517.2 , XM_012965504.2 , XM_012965540.2 , XM_012965505.2 , XM_012965500.2 , XM_012965513.2 , XM_012965521.1
    Accession Version XM_012965504.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820958.1
    CDS586..3987
    Translation

    Target ORF information:

    RefSeq Version XM_012965504.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965504.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29362
    Clone ID Related Accession (Same CDS sequence) XM_018094514.1 , XM_012965517.2 , XM_012965504.2 , XM_012965540.2 , XM_012965505.2 , XM_012965500.2 , XM_012965513.2 , XM_012965521.1
    Accession Version XM_012965540.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820994.1
    CDS309..3710
    Translation

    Target ORF information:

    RefSeq Version XM_012965540.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965540.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29362
    Clone ID Related Accession (Same CDS sequence) XM_018094514.1 , XM_012965517.2 , XM_012965504.2 , XM_012965540.2 , XM_012965505.2 , XM_012965500.2 , XM_012965513.2 , XM_012965521.1
    Accession Version XM_012965505.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965505.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820959.1
    CDS549..3950
    Translation

    Target ORF information:

    RefSeq Version XM_012965505.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965505.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29362
    Clone ID Related Accession (Same CDS sequence) XM_018094514.1 , XM_012965517.2 , XM_012965504.2 , XM_012965540.2 , XM_012965505.2 , XM_012965500.2 , XM_012965513.2 , XM_012965521.1
    Accession Version XM_012965500.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965500.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820954.1
    CDS498..3899
    Translation

    Target ORF information:

    RefSeq Version XM_012965500.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965500.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29362
    Clone ID Related Accession (Same CDS sequence) XM_018094514.1 , XM_012965517.2 , XM_012965504.2 , XM_012965540.2 , XM_012965505.2 , XM_012965500.2 , XM_012965513.2 , XM_012965521.1
    Accession Version XM_012965513.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820967.1
    CDS460..3861
    Translation

    Target ORF information:

    RefSeq Version XM_012965513.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965513.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29362
    Clone ID Related Accession (Same CDS sequence) XM_018094514.1 , XM_012965517.2 , XM_012965504.2 , XM_012965540.2 , XM_012965505.2 , XM_012965500.2 , XM_012965513.2 , XM_012965521.1
    Accession Version XM_012965521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820975.1
    CDS268..3669
    Translation

    Target ORF information:

    RefSeq Version XM_012965521.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGATCGG
    AGCCAAACAT CAGTAACAGA GGAGAACAGT AAGCTGCTAG ACATTGACCG CCGGAAGAGT
    TCAGTGCTTT ATCAAAATCA CACCGATGAT GAAGGGGACT CTTATGACAG AAATCTGGCC
    CTGTTTGAGG AAGAAATGGA TACTCGACCA AAAGTTTCAT CCCTCCTTAA CCGCTTGGCT
    AATTACACAA ATCTGCCTCA GGGAGCAAAA GAACATGAAG AGGAAAGTGA GGGCAAGAAG
    AAGGCTGCTA AAACCCCCCG AATGGGCACG ATAATGGGGG TTTACCTCCC TTGTCTTCAG
    AACATTTTTG GGGTCATTTT ATTCCTTCGA CTCCCGTGGG TGGTGGGCAC AGCAGGAGTC
    TTGCATGCCT TCTGCATTGT ATTCGCCTGC TGCTGCTGTA CCATGCTGAC GGCTATTTCT
    ATGAGCGCCA TTGCAACAAA TGGGGTGGTG CCAGCTGGTG GTGCCTATTT CATGATTTCT
    CGAGCCCTTG GTCCAGAGTT TGGAGGTGCT GTAGGCCTGT GCTTCTACCT TGGAACAACC
    TTTGCAACAG CTATGTACAT CCTGGGGGCT ATTGAGATAT TCCTGGTGTA CATTTCCCCC
    CAAGCAGTCA TATTCCATGG AGAGGGGGTG GCAGAGGAAT CAGCAGCAAT GTTGAATAAT
    ATGCGTGTAT ATGGAACTGG ATTCCTGATT ATTATGTCTA TTATTGTGTT TGTTGGAGTG
    CGATATGTAA ACAAGTTGGC CTCTGTATTC CTGACCTGTG TCATCATGTC TATTCTGGCG
    ATTTATGCTG GGGCACTAAA ATCTGCATTT TCTCCCCCGG ACTTTCCGAT TTGCTTGCTT
    GGAAACCGGA CTCTGTCCAA GCATCATTTT ACCGAATGTG CCAAGATAGT GGAGAGCGAC
    AACATGACCA TTGTCACAGA TCTGTGGAAT CTATTCTGCC ACCCATCGGA TCAGCTGAAT
    GGAACTTGTG ATGAATATTT TGCCCATAAT AATGTCACCT TAGTCCAAGG AATCCCAGGC
    TTAAAGAGCG GGATCATCTC TGAAAACCTG TGGAGCAATT ACCTTCAAAA GGGAGACATA
    ATAGAAAAGG CTTCATTGCC ATCAACTGAG TATTTGGCTG TGCAGTCTCA AGAGTATGTT
    TTAGCTGACA TTACGACTTC TTTTACCCTG TTGGTTGGAA TATTTTTCCC ATCTGTAACA
    GGAATTATGG CTGGATCAAA TCGCTCTGGA GACCTCAAGG ATGCCCAGAA ATCGATTCCA
    ATTGGAACTA TTCTTGCAAT TCTTACAACC TCCTTAGTCT ACCTCAGTAA TGTCATCCTG
    TTTGGAGCTT CTATAGATGG AGTTGTGCTC AGAGACAAGT TTGGGGATGC AGTGAAAGGA
    ACTCTTGTTG TTGGTGCGCT TTCATGGCCA TCTCCTTGGG TCATTGTGAT TGGATCTTTT
    TTCTCTACTT GTGGAGCTGG ACTTCAAAGT TTGACTGGAG CACCTCGCCT GCTCCAAGCC
    ATTGCTAAGG ATGGAATCAT TCCATTTCTG AGGGTATTCG GACACGGAAA GAGTAATGGG
    GAACCGACAT GGGCTCTTCT TCTAACGGCA GTGATTGCTG AGCTGGGGAT CCTTATTGCT
    TCTCTGGACT TGGTGGCACC TATCCTATCT ATGTTCTTTC TAATGTGTTA CCTTTTTGTA
    AACTTGGCAT GTGGATTACA GACCCTTCTC CATTCACCCA ACTGGCGCCC TCGCTTCCAT
    TATTATCACT GGACTTTATC TTTTCTTGGA ATGGCACTGT GTTTAGCCCT CATGTTTATC
    TCTTCCTGGT ATTATGCCCT AATTTCCATG GTAATTGCTG GAATGATCTA CAAGTACATT
    GAATACCAAG GGGCCGAAAA AGAATGGGGA GATGGAATCC GTGGATTGTC CTTGAGTGCA
    GCACGTTTTG CTCTCCTGCG CCTGGAAGAG GGTCCACCTC ACACTAAGAA CTGGAGACCT
    CAGCTACTTG TTTTGGTGAA ATTGGATTCA GATCTCCATG TCTCTCAGCC TCGCCTTCTT
    TCCTTCGCCT CCCAGCTAAA GGCTGGTAAA GGTCTGACCA TTGTGGGTTC AGTACTTCTG
    GGAGACTACC TAGAGAATCA TGCAGAGGCC CAGGCAGCGG AGCAGGCACT GAAACACTTA
    ATGGAGCAAG AAAAAGTAAA AGGTTTTTGC CAAGTGGTTG TGGCTCAGAA GCTGAAAGAG
    GGTCTTTCCC ACCTTATACA GTCCTGTGGT CTTGGGGGAA TGAGACACAA CACTGTGATC
    ATGAGCTGGC CAAGTAGCTG GAGACAGAGT GATGACTCAC GGGCTTGGAA ATCCTTCATC
    ACTACCATTC GTGTAACCAC AGCTGCCCGC CAAGCACTTC TGGTAGCAAA GAATGTATCC
    CTGTTCCCTG GCTCCCGTGA AATATTAGCA GAAGGGCACA TTGATGTTTG GTGGATAGTA
    CATGATGGGG GCATGCTCAT GCTTCTGCCC TTCCTCCTTA AACAACACAA GGTGTGGCGG
    AAATGTAAGA TGCGTATTTT CACTGTGGCT CAGATGGAAG ACAATAGTAT TCAGATGAAA
    AAGGACTTGG CTACCTTCCT TTATCATTTA CGTATCGCTG CTGATGTTGA AGTGGTGGAG
    ATGCATGACA GCGATATATC TGCATACACA TATGAACGCA CACTCATGAT GGAGCAGAGG
    TCACAAATGC TGAGACAGAT GCGCCTTTCA AAAACAGATC GGGAAAAGGA GGCTCAGTTG
    GTGAAGGACA GGAATTCAAT GCTGCGTTTA ACCAGTGTGG GCTCTGATGA TGATGAAGAT
    ACAGAGGCTG TCCCAGATAG AGTGCACATG ACTTGGACCA AGGATAAGCA CCATGCAGTT
    CGTGTTGCCC AAAGCAAACC AATGCCCAGC TTTCAGGACC TTCTCAACAT ACGACCGGAC
    CAGTCGAATG TTCGGCGTAT GCACACAGCT GTGAAACTTA ATGAAGTCAT TGTTAATAAA
    TCCCATGATG CCAAATTGGT TTTGCTCAAC ATGCCAGGAC CCCCTCGTAA CCCTCAGGGT
    GATGAGAATT ATATGGAGTT TCTAGAGGTG CTTACTGAGG GCTTGGAACA CGTGCTGTTA
    GTGAGAGGAG GTGGAACAGA GGTGATCACC ATTTACTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29363
    Clone ID Related Accession (Same CDS sequence) XM_018094520.1
    Accession Version XM_018094520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGACATT
    GACCGCCGGA AGAGTTCAGT GCTTTATCAA AATCACACCG ATGATGAAGG GGACTCTTAT
    GACAGAAATC TGGCCCTGTT TGAGGAAGAA ATGGATACTC GACCAAAAGT TTCATCCCTC
    CTTAACCGCT TGGCTAATTA CACAAATCTG CCTCAGGGAG CAAAAGAACA TGAAGAGGAA
    AGTGAGGGCA AGAAGAAGGC TGCTAAAACC CCCCGAATGG GCACGATAAT GGGGGTTTAC
    CTCCCTTGTC TTCAGAACAT TTTTGGGGTC ATTTTATTCC TTCGACTCCC GTGGGTGGTG
    GGCACAGCAG GAGTCTTGCA TGCCTTCTGC ATTGTATTCG CCTGCTGCTG CTGTACCATG
    CTGACGGCTA TTTCTATGAG CGCCATTGCA ACAAATGGGG TGGTGCCAGC TGGTGGTGCC
    TATTTCATGA TTTCTCGAGC CCTTGGTCCA GAGTTTGGAG GTGCTGTAGG CCTGTGCTTC
    TACCTTGGAA CAACCTTTGC AACAGCTATG TACATCCTGG GGGCTATTGA GATATTCCTG
    GTGTACATTT CCCCCCAAGC AGTCATATTC CATGGAGAGG GGGTGGCAGA GGAATCAGCA
    GCAATGTTGA ATAATATGCG TGTATATGGA ACTGGATTCC TGATTATTAT GTCTATTATT
    GTGTTTGTTG GAGTGCGATA TGTAAACAAG TTGGCCTCTG TATTCCTGAC CTGTGTCATC
    ATGTCTATTC TGGCGATTTA TGCTGGGGCA CTAAAATCTG CATTTTCTCC CCCGGACTTT
    CCGATTTGCT TGCTTGGAAA CCGGACTCTG TCCAAGCATC ATTTTACCGA ATGTGCCAAG
    ATAGTGGAGA GCGACAACAT GACCATTGTC ACAGATCTGT GGAATCTATT CTGCCACCCA
    TCGGATCAGC TGAATGGAAC TTGTGATGAA TATTTTGCCC ATAATAATGT CACCTTAGTC
    CAAGGAATCC CAGGCTTAAA GAGCGGGATC ATCTCTGAAA ACCTGTGGAG CAATTACCTT
    CAAAAGGGAG ACATAATAGA AAAGGCTTCA TTGCCATCAA CTGAGTATTT GGCTGTGCAG
    TCTCAAGAGT ATGTTTTAGC TGACATTACG ACTTCTTTTA CCCTGTTGGT TGGAATATTT
    TTCCCATCTG TAACAGGAAT TATGGCTGGA TCAAATCGCT CTGGAGACCT CAAGGATGCC
    CAGAAATCGA TTCCAATTGG AACTATTCTT GCAATTCTTA CAACCTCCTT AGTCTACCTC
    AGTAATGTCA TCCTGTTTGG AGCTTCTATA GATGGAGTTG TGCTCAGAGA CAAGTTTGGG
    GATGCAGTGA AAGGAACTCT TGTTGTTGGT GCGCTTTCAT GGCCATCTCC TTGGGTCATT
    GTGATTGGAT CTTTTTTCTC TACTTGTGGA GCTGGACTTC AAAGTTTGAC TGGAGCACCT
    CGCCTGCTCC AAGCCATTGC TAAGGATGGA ATCATTCCAT TTCTGAGGGT ATTCGGACAC
    GGAAAGAGTA ATGGGGAACC GACATGGGCT CTTCTTCTAA CGGCAGTGAT TGCTGAGCTG
    GGGATCCTTA TTGCTTCTCT GGACTTGGTG GCACCTATCC TATCTATGTT CTTTCTAATG
    TGTTACCTTT TTGTAAACTT GGCATGTGGA TTACAGACCC TTCTCCATTC ACCCAACTGG
    CGCCCTCGCT TCCATTATTA TCACTGGACT TTATCTTTTC TTGGAATGGC ACTGTGTTTA
    GCCCTCATGT TTATCTCTTC CTGGTATTAT GCCCTAATTT CCATGGTAAT TGCTGGAATG
    ATCTACAAGT ACATTGAATA CCAAGGGGCC GAAAAAGAAT GGGGAGATGG AATCCGTGGA
    TTGTCCTTGA GTGCAGCACG TTTTGCTCTC CTGCGCCTGG AAGAGGGTCC ACCTCACACT
    AAGAACTGGA GACCTCAGCT ACTTGTTTTG GTGAAATTGG ATTCAGATCT CCATGTCTCT
    CAGCCTCGCC TTCTTTCCTT CGCCTCCCAG CTAAAGGCTG GTAAAGGTCT GACCATTGTG
    GGTTCAGTAC TTCTGGGAGA CTACCTAGAG AATCATGCAG AGGCCCAGGC AGCGGAGCAG
    GCACTGAAAC ACTTAATGGA GCAAGAAAAA GTAAAAGGTT TTTGCCAAGT GGTTGTGGCT
    CAGAAGCTGA AAGAGGGTCT TTCCCACCTT ATACAGTCCT GTGGTCTTGG GGGAATGAGA
    CACAACACTG TGATCATGAG CTGGCCAAGT AGCTGGAGAC AGAGTGATGA CTCACGGGCT
    TGGAAATCCT TCATCACTAC CATTCGTGTA ACCACAGCTG CCCGCCAAGC ACTTCTGGTA
    GCAAAGAATG TATCCCTGTT CCCTGGCTCC CGTGAAATAT TAGCAGAAGG GCACATTGAT
    GTTTGGTGGA TAGTACATGA TGGGGGCATG CTCATGCTTC TGCCCTTCCT CCTTAAACAA
    CACAAGGTGT GGCGGAAATG TAAGATGCGT ATTTTCACTG TGGCTCAGAT GGAAGACAAT
    AGTATTCAGA TGAAAAAGGA CTTGGCTACC TTCCTTTATC ATTTACGTAT CGCTGCTGAT
    GTTGAAGTGG TGGAGATGCA TGACAGCGAT ATATCTGCAT ACACATATGA ACGCACACTC
    ATGATGGAGC AGAGGTCACA AATGCTGAGA CAGATGCGCC TTTCAAAAAC AGATCGGGAA
    AAGGAGGCTC AGTTGGTGAA GGACAGGAAT TCAATGCTGC GTTTAACCAG TGTGGGCTCT
    GATGATGATG AAGATACAGA GGCTGTCCCA GATAGAGTGC ACATGACTTG GACCAAGGAT
    AAGCACCATG CAGTTCGTGT TGCCCAAAGC AAACCAATGC CCAGCTTTCA GGACCTTCTC
    AACATACGAC CGGACCAGTC GAATGTTCGG CGTATGCACA CAGCTGTGAA ACTTAATGAA
    GTCATTGTTA ATAAATCCCA TGATGCCAAA TTGGTTTTGC TCAACATGCC AGGACCCCCT
    CGTAACCCTC AGGGTGATGA GAATTATATG GAGTTTCTAG AGGTGCTTAC TGAGGGCTTG
    GAACACGTGC TGTTAGTGAG AGGAGGTGGA ACAGAGGTGA TCACCATTTA CTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950009.1
    CDS587..3943
    Translation

    Target ORF information:

    RefSeq Version XM_018094520.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018094520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCACCCA AAATGGCATC GGTACGGTTC ACCGTTACAC CCACCAAAAT GGACGAAATG 
    GGGGCAATCT CTGACACCAG CCCAGATCTC AGTTCATCCT CCCGTGTGCG GTTTAGCTCC
    AGAGAGAGTG TCAGAGATGC CAGCCGCAGT GAGGCTTACA GTGACACCTC ACTGGCAACC
    ACAGGAGTGG ACCAAGCTAG TGAAAGGACC TCCAACCCTA TGGACAGTGG AGAAGACATT
    GACCGCCGGA AGAGTTCAGT GCTTTATCAA AATCACACCG ATGATGAAGG GGACTCTTAT
    GACAGAAATC TGGCCCTGTT TGAGGAAGAA ATGGATACTC GACCAAAAGT TTCATCCCTC
    CTTAACCGCT TGGCTAATTA CACAAATCTG CCTCAGGGAG CAAAAGAACA TGAAGAGGAA
    AGTGAGGGCA AGAAGAAGGC TGCTAAAACC CCCCGAATGG GCACGATAAT GGGGGTTTAC
    CTCCCTTGTC TTCAGAACAT TTTTGGGGTC ATTTTATTCC TTCGACTCCC GTGGGTGGTG
    GGCACAGCAG GAGTCTTGCA TGCCTTCTGC ATTGTATTCG CCTGCTGCTG CTGTACCATG
    CTGACGGCTA TTTCTATGAG CGCCATTGCA ACAAATGGGG TGGTGCCAGC TGGTGGTGCC
    TATTTCATGA TTTCTCGAGC CCTTGGTCCA GAGTTTGGAG GTGCTGTAGG CCTGTGCTTC
    TACCTTGGAA CAACCTTTGC AACAGCTATG TACATCCTGG GGGCTATTGA GATATTCCTG
    GTGTACATTT CCCCCCAAGC AGTCATATTC CATGGAGAGG GGGTGGCAGA GGAATCAGCA
    GCAATGTTGA ATAATATGCG TGTATATGGA ACTGGATTCC TGATTATTAT GTCTATTATT
    GTGTTTGTTG GAGTGCGATA TGTAAACAAG TTGGCCTCTG TATTCCTGAC CTGTGTCATC
    ATGTCTATTC TGGCGATTTA TGCTGGGGCA CTAAAATCTG CATTTTCTCC CCCGGACTTT
    CCGATTTGCT TGCTTGGAAA CCGGACTCTG TCCAAGCATC ATTTTACCGA ATGTGCCAAG
    ATAGTGGAGA GCGACAACAT GACCATTGTC ACAGATCTGT GGAATCTATT CTGCCACCCA
    TCGGATCAGC TGAATGGAAC TTGTGATGAA TATTTTGCCC ATAATAATGT CACCTTAGTC
    CAAGGAATCC CAGGCTTAAA GAGCGGGATC ATCTCTGAAA ACCTGTGGAG CAATTACCTT
    CAAAAGGGAG ACATAATAGA AAAGGCTTCA TTGCCATCAA CTGAGTATTT GGCTGTGCAG
    TCTCAAGAGT ATGTTTTAGC TGACATTACG ACTTCTTTTA CCCTGTTGGT TGGAATATTT
    TTCCCATCTG TAACAGGAAT TATGGCTGGA TCAAATCGCT CTGGAGACCT CAAGGATGCC
    CAGAAATCGA TTCCAATTGG AACTATTCTT GCAATTCTTA CAACCTCCTT AGTCTACCTC
    AGTAATGTCA TCCTGTTTGG AGCTTCTATA GATGGAGTTG TGCTCAGAGA CAAGTTTGGG
    GATGCAGTGA AAGGAACTCT TGTTGTTGGT GCGCTTTCAT GGCCATCTCC TTGGGTCATT
    GTGATTGGAT CTTTTTTCTC TACTTGTGGA GCTGGACTTC AAAGTTTGAC TGGAGCACCT
    CGCCTGCTCC AAGCCATTGC TAAGGATGGA ATCATTCCAT TTCTGAGGGT ATTCGGACAC
    GGAAAGAGTA ATGGGGAACC GACATGGGCT CTTCTTCTAA CGGCAGTGAT TGCTGAGCTG
    GGGATCCTTA TTGCTTCTCT GGACTTGGTG GCACCTATCC TATCTATGTT CTTTCTAATG
    TGTTACCTTT TTGTAAACTT GGCATGTGGA TTACAGACCC TTCTCCATTC ACCCAACTGG
    CGCCCTCGCT TCCATTATTA TCACTGGACT TTATCTTTTC TTGGAATGGC ACTGTGTTTA
    GCCCTCATGT TTATCTCTTC CTGGTATTAT GCCCTAATTT CCATGGTAAT TGCTGGAATG
    ATCTACAAGT ACATTGAATA CCAAGGGGCC GAAAAAGAAT GGGGAGATGG AATCCGTGGA
    TTGTCCTTGA GTGCAGCACG TTTTGCTCTC CTGCGCCTGG AAGAGGGTCC ACCTCACACT
    AAGAACTGGA GACCTCAGCT ACTTGTTTTG GTGAAATTGG ATTCAGATCT CCATGTCTCT
    CAGCCTCGCC TTCTTTCCTT CGCCTCCCAG CTAAAGGCTG GTAAAGGTCT GACCATTGTG
    GGTTCAGTAC TTCTGGGAGA CTACCTAGAG AATCATGCAG AGGCCCAGGC AGCGGAGCAG
    GCACTGAAAC ACTTAATGGA GCAAGAAAAA GTAAAAGGTT TTTGCCAAGT GGTTGTGGCT
    CAGAAGCTGA AAGAGGGTCT TTCCCACCTT ATACAGTCCT GTGGTCTTGG GGGAATGAGA
    CACAACACTG TGATCATGAG CTGGCCAAGT AGCTGGAGAC AGAGTGATGA CTCACGGGCT
    TGGAAATCCT TCATCACTAC CATTCGTGTA ACCACAGCTG CCCGCCAAGC ACTTCTGGTA
    GCAAAGAATG TATCCCTGTT CCCTGGCTCC CGTGAAATAT TAGCAGAAGG GCACATTGAT
    GTTTGGTGGA TAGTACATGA TGGGGGCATG CTCATGCTTC TGCCCTTCCT CCTTAAACAA
    CACAAGGTGT GGCGGAAATG TAAGATGCGT ATTTTCACTG TGGCTCAGAT GGAAGACAAT
    AGTATTCAGA TGAAAAAGGA CTTGGCTACC TTCCTTTATC ATTTACGTAT CGCTGCTGAT
    GTTGAAGTGG TGGAGATGCA TGACAGCGAT ATATCTGCAT ACACATATGA ACGCACACTC
    ATGATGGAGC AGAGGTCACA AATGCTGAGA CAGATGCGCC TTTCAAAAAC AGATCGGGAA
    AAGGAGGCTC AGTTGGTGAA GGACAGGAAT TCAATGCTGC GTTTAACCAG TGTGGGCTCT
    GATGATGATG AAGATACAGA GGCTGTCCCA GATAGAGTGC ACATGACTTG GACCAAGGAT
    AAGCACCATG CAGTTCGTGT TGCCCAAAGC AAACCAATGC CCAGCTTTCA GGACCTTCTC
    AACATACGAC CGGACCAGTC GAATGTTCGG CGTATGCACA CAGCTGTGAA ACTTAATGAA
    GTCATTGTTA ATAAATCCCA TGATGCCAAA TTGGTTTTGC TCAACATGCC AGGACCCCCT
    CGTAACCCTC AGGGTGATGA GAATTATATG GAGTTTCTAG AGGTGCTTAC TGAGGGCTTG
    GAACACGTGC TGTTAGTGAG AGGAGGTGGA ACAGAGGTGA TCACCATTTA CTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa29364
    Clone ID Related Accession (Same CDS sequence) XM_012965533.2
    Accession Version XM_012965533.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product solute carrier family 12 member 6 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012965533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCACATT TTACAGTAAC TAAAGTACCT GACCCTCACT GTGCTACCGA GGAGGCGACC 
    TCCAGTCCTA CGAGCTGGGC TGACAGTGAG GAGGATTCAT GGGGAGCTGA TCGGAGCCAA
    ACATCAGTAA CAGAGGAGAA CAGTAAGCTG CTAGACATTG ACCGCCGGAA GAGTTCAGTG
    CTTTATCAAA ATCACACCGA TGATGAAGGG GACTCTTATG ACAGAAATCT GGCCCTGTTT
    GAGGAAGAAA TGGATACTCG ACCAAAAGTT TCATCCCTCC TTAACCGCTT GGCTAATTAC
    ACAAATCTGC CTCAGGGAGC AAAAGAACAT GAAGAGGAAA GTGAGGGCAA GAAGAAGGCT
    GCTAAAACCC CCCGAATGGG CACGATAATG GGGGTTTACC TCCCTTGTCT TCAGAACATT
    TTTGGGGTCA TTTTATTCCT TCGACTCCCG TGGGTGGTGG GCACAGCAGG AGTCTTGCAT
    GCCTTCTGCA TTGTATTCGC CTGCTGCTGC TGTACCATGC TGACGGCTAT TTCTATGAGC
    GCCATTGCAA CAAATGGGGT GGTGCCAGCT GGTGGTGCCT ATTTCATGAT TTCTCGAGCC
    CTTGGTCCAG AGTTTGGAGG TGCTGTAGGC CTGTGCTTCT ACCTTGGAAC AACCTTTGCA
    ACAGCTATGT ACATCCTGGG GGCTATTGAG ATATTCCTGG TGTACATTTC CCCCCAAGCA
    GTCATATTCC ATGGAGAGGG GGTGGCAGAG GAATCAGCAG CAATGTTGAA TAATATGCGT
    GTATATGGAA CTGGATTCCT GATTATTATG TCTATTATTG TGTTTGTTGG AGTGCGATAT
    GTAAACAAGT TGGCCTCTGT ATTCCTGACC TGTGTCATCA TGTCTATTCT GGCGATTTAT
    GCTGGGGCAC TAAAATCTGC ATTTTCTCCC CCGGACTTTC CGATTTGCTT GCTTGGAAAC
    CGGACTCTGT CCAAGCATCA TTTTACCGAA TGTGCCAAGA TAGTGGAGAG CGACAACATG
    ACCATTGTCA CAGATCTGTG GAATCTATTC TGCCACCCAT CGGATCAGCT GAATGGAACT
    TGTGATGAAT ATTTTGCCCA TAATAATGTC ACCTTAGTCC AAGGAATCCC AGGCTTAAAG
    AGCGGGATCA TCTCTGAAAA CCTGTGGAGC AATTACCTTC AAAAGGGAGA CATAATAGAA
    AAGGCTTCAT TGCCATCAAC TGAGTATTTG GCTGTGCAGT CTCAAGAGTA TGTTTTAGCT
    GACATTACGA CTTCTTTTAC CCTGTTGGTT GGAATATTTT TCCCATCTGT AACAGGAATT
    ATGGCTGGAT CAAATCGCTC TGGAGACCTC AAGGATGCCC AGAAATCGAT TCCAATTGGA
    ACTATTCTTG CAATTCTTAC AACCTCCTTA GTCTACCTCA GTAATGTCAT CCTGTTTGGA
    GCTTCTATAG ATGGAGTTGT GCTCAGAGAC AAGTTTGGGG ATGCAGTGAA AGGAACTCTT
    GTTGTTGGTG CGCTTTCATG GCCATCTCCT TGGGTCATTG TGATTGGATC TTTTTTCTCT
    ACTTGTGGAG CTGGACTTCA AAGTTTGACT GGAGCACCTC GCCTGCTCCA AGCCATTGCT
    AAGGATGGAA TCATTCCATT TCTGAGGGTA TTCGGACACG GAAAGAGTAA TGGGGAACCG
    ACATGGGCTC TTCTTCTAAC GGCAGTGATT GCTGAGCTGG GGATCCTTAT TGCTTCTCTG
    GACTTGGTGG CACCTATCCT ATCTATGTTC TTTCTAATGT GTTACCTTTT TGTAAACTTG
    GCATGTGGAT TACAGACCCT TCTCCATTCA CCCAACTGGC GCCCTCGCTT CCATTATTAT
    CACTGGACTT TATCTTTTCT TGGAATGGCA CTGTGTTTAG CCCTCATGTT TATCTCTTCC
    TGGTATTATG CCCTAATTTC CATGGTAATT GCTGGAATGA TCTACAAGTA CATTGAATAC
    CAAGGGGCCG AAAAAGAATG GGGAGATGGA ATCCGTGGAT TGTCCTTGAG TGCAGCACGT
    TTTGCTCTCC TGCGCCTGGA AGAGGGTCCA CCTCACACTA AGAACTGGAG ACCTCAGCTA
    CTTGTTTTGG TGAAATTGGA TTCAGATCTC CATGTCTCTC AGCCTCGCCT TCTTTCCTTC
    GCCTCCCAGC TAAAGGCTGG TAAAGGTCTG ACCATTGTGG GTTCAGTACT TCTGGGAGAC
    TACCTAGAGA ATCATGCAGA GGCCCAGGCA GCGGAGCAGG CACTGAAACA CTTAATGGAG
    CAAGAAAAAG TAAAAGGTTT TTGCCAAGTG GTTGTGGCTC AGAAGCTGAA AGAGGGTCTT
    TCCCACCTTA TACAGTCCTG TGGTCTTGGG GGAATGAGAC ACAACACTGT GATCATGAGC
    TGGCCAAGTA GCTGGAGACA GAGTGATGAC TCACGGGCTT GGAAATCCTT CATCACTACC
    ATTCGTGTAA CCACAGCTGC CCGCCAAGCA CTTCTGGTAG CAAAGAATGT ATCCCTGTTC
    CCTGGCTCCC GTGAAATATT AGCAGAAGGG CACATTGATG TTTGGTGGAT AGTACATGAT
    GGGGGCATGC TCATGCTTCT GCCCTTCCTC CTTAAACAAC ACAAGGTGTG GCGGAAATGT
    AAGATGCGTA TTTTCACTGT GGCTCAGATG GAAGACAATA GTATTCAGAT GAAAAAGGAC
    TTGGCTACCT TCCTTTATCA TTTACGTATC GCTGCTGATG TTGAAGTGGT GGAGATGCAT
    GACAGCGATA TATCTGCATA CACATATGAA CGCACACTCA TGATGGAGCA GAGGTCACAA
    ATGCTGAGAC AGATGCGCCT TTCAAAAACA GATCGGGAAA AGGAGGCTCA GTTGGTGAAG
    GACAGGAATT CAATGCTGCG TTTAACCAGT GTGGGCTCTG ATGATGATGA AGATACAGAG
    GCTGTCCCAG ATAGAGTGCA CATGACTTGG ACCAAGGATA AGCACCATGC AGTTCGTGTT
    GCCCAAAGCA AACCAATGCC CAGCTTTCAG GACCTTCTCA ACATACGACC GGACCAGTCG
    AATGTTCGGC GTATGCACAC AGCTGTGAAA CTTAATGAAG TCATTGTTAA TAAATCCCAT
    GATGCCAAAT TGGTTTTGCT CAACATGCCA GGACCCCCTC GTAACCCTCA GGGTGATGAG
    AATTATATGG AGTTTCTAGA GGTGCTTACT GAGGGCTTGG AACACGTGCT GTTAGTGAGA
    GGAGGTGGAA CAGAGGTGAT CACCATTTAC TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012820987.1
    CDS2991..6266
    Translation

    Target ORF information:

    RefSeq Version XM_012965533.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis solute carrier family 12 (potassium/chloride transporter), member 6 (slc12a6), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012965533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCACATT TTACAGTAAC TAAAGTACCT GACCCTCACT GTGCTACCGA GGAGGCGACC 
    TCCAGTCCTA CGAGCTGGGC TGACAGTGAG GAGGATTCAT GGGGAGCTGA TCGGAGCCAA
    ACATCAGTAA CAGAGGAGAA CAGTAAGCTG CTAGACATTG ACCGCCGGAA GAGTTCAGTG
    CTTTATCAAA ATCACACCGA TGATGAAGGG GACTCTTATG ACAGAAATCT GGCCCTGTTT
    GAGGAAGAAA TGGATACTCG ACCAAAAGTT TCATCCCTCC TTAACCGCTT GGCTAATTAC
    ACAAATCTGC CTCAGGGAGC AAAAGAACAT GAAGAGGAAA GTGAGGGCAA GAAGAAGGCT
    GCTAAAACCC CCCGAATGGG CACGATAATG GGGGTTTACC TCCCTTGTCT TCAGAACATT
    TTTGGGGTCA TTTTATTCCT TCGACTCCCG TGGGTGGTGG GCACAGCAGG AGTCTTGCAT
    GCCTTCTGCA TTGTATTCGC CTGCTGCTGC TGTACCATGC TGACGGCTAT TTCTATGAGC
    GCCATTGCAA CAAATGGGGT GGTGCCAGCT GGTGGTGCCT ATTTCATGAT TTCTCGAGCC
    CTTGGTCCAG AGTTTGGAGG TGCTGTAGGC CTGTGCTTCT ACCTTGGAAC AACCTTTGCA
    ACAGCTATGT ACATCCTGGG GGCTATTGAG ATATTCCTGG TGTACATTTC CCCCCAAGCA
    GTCATATTCC ATGGAGAGGG GGTGGCAGAG GAATCAGCAG CAATGTTGAA TAATATGCGT
    GTATATGGAA CTGGATTCCT GATTATTATG TCTATTATTG TGTTTGTTGG AGTGCGATAT
    GTAAACAAGT TGGCCTCTGT ATTCCTGACC TGTGTCATCA TGTCTATTCT GGCGATTTAT
    GCTGGGGCAC TAAAATCTGC ATTTTCTCCC CCGGACTTTC CGATTTGCTT GCTTGGAAAC
    CGGACTCTGT CCAAGCATCA TTTTACCGAA TGTGCCAAGA TAGTGGAGAG CGACAACATG
    ACCATTGTCA CAGATCTGTG GAATCTATTC TGCCACCCAT CGGATCAGCT GAATGGAACT
    TGTGATGAAT ATTTTGCCCA TAATAATGTC ACCTTAGTCC AAGGAATCCC AGGCTTAAAG
    AGCGGGATCA TCTCTGAAAA CCTGTGGAGC AATTACCTTC AAAAGGGAGA CATAATAGAA
    AAGGCTTCAT TGCCATCAAC TGAGTATTTG GCTGTGCAGT CTCAAGAGTA TGTTTTAGCT
    GACATTACGA CTTCTTTTAC CCTGTTGGTT GGAATATTTT TCCCATCTGT AACAGGAATT
    ATGGCTGGAT CAAATCGCTC TGGAGACCTC AAGGATGCCC AGAAATCGAT TCCAATTGGA
    ACTATTCTTG CAATTCTTAC AACCTCCTTA GTCTACCTCA GTAATGTCAT CCTGTTTGGA
    GCTTCTATAG ATGGAGTTGT GCTCAGAGAC AAGTTTGGGG ATGCAGTGAA AGGAACTCTT
    GTTGTTGGTG CGCTTTCATG GCCATCTCCT TGGGTCATTG TGATTGGATC TTTTTTCTCT
    ACTTGTGGAG CTGGACTTCA AAGTTTGACT GGAGCACCTC GCCTGCTCCA AGCCATTGCT
    AAGGATGGAA TCATTCCATT TCTGAGGGTA TTCGGACACG GAAAGAGTAA TGGGGAACCG
    ACATGGGCTC TTCTTCTAAC GGCAGTGATT GCTGAGCTGG GGATCCTTAT TGCTTCTCTG
    GACTTGGTGG CACCTATCCT ATCTATGTTC TTTCTAATGT GTTACCTTTT TGTAAACTTG
    GCATGTGGAT TACAGACCCT TCTCCATTCA CCCAACTGGC GCCCTCGCTT CCATTATTAT
    CACTGGACTT TATCTTTTCT TGGAATGGCA CTGTGTTTAG CCCTCATGTT TATCTCTTCC
    TGGTATTATG CCCTAATTTC CATGGTAATT GCTGGAATGA TCTACAAGTA CATTGAATAC
    CAAGGGGCCG AAAAAGAATG GGGAGATGGA ATCCGTGGAT TGTCCTTGAG TGCAGCACGT
    TTTGCTCTCC TGCGCCTGGA AGAGGGTCCA CCTCACACTA AGAACTGGAG ACCTCAGCTA
    CTTGTTTTGG TGAAATTGGA TTCAGATCTC CATGTCTCTC AGCCTCGCCT TCTTTCCTTC
    GCCTCCCAGC TAAAGGCTGG TAAAGGTCTG ACCATTGTGG GTTCAGTACT TCTGGGAGAC
    TACCTAGAGA ATCATGCAGA GGCCCAGGCA GCGGAGCAGG CACTGAAACA CTTAATGGAG
    CAAGAAAAAG TAAAAGGTTT TTGCCAAGTG GTTGTGGCTC AGAAGCTGAA AGAGGGTCTT
    TCCCACCTTA TACAGTCCTG TGGTCTTGGG GGAATGAGAC ACAACACTGT GATCATGAGC
    TGGCCAAGTA GCTGGAGACA GAGTGATGAC TCACGGGCTT GGAAATCCTT CATCACTACC
    ATTCGTGTAA CCACAGCTGC CCGCCAAGCA CTTCTGGTAG CAAAGAATGT ATCCCTGTTC
    CCTGGCTCCC GTGAAATATT AGCAGAAGGG CACATTGATG TTTGGTGGAT AGTACATGAT
    GGGGGCATGC TCATGCTTCT GCCCTTCCTC CTTAAACAAC ACAAGGTGTG GCGGAAATGT
    AAGATGCGTA TTTTCACTGT GGCTCAGATG GAAGACAATA GTATTCAGAT GAAAAAGGAC
    TTGGCTACCT TCCTTTATCA TTTACGTATC GCTGCTGATG TTGAAGTGGT GGAGATGCAT
    GACAGCGATA TATCTGCATA CACATATGAA CGCACACTCA TGATGGAGCA GAGGTCACAA
    ATGCTGAGAC AGATGCGCCT TTCAAAAACA GATCGGGAAA AGGAGGCTCA GTTGGTGAAG
    GACAGGAATT CAATGCTGCG TTTAACCAGT GTGGGCTCTG ATGATGATGA AGATACAGAG
    GCTGTCCCAG ATAGAGTGCA CATGACTTGG ACCAAGGATA AGCACCATGC AGTTCGTGTT
    GCCCAAAGCA AACCAATGCC CAGCTTTCAG GACCTTCTCA ACATACGACC GGACCAGTCG
    AATGTTCGGC GTATGCACAC AGCTGTGAAA CTTAATGAAG TCATTGTTAA TAAATCCCAT
    GATGCCAAAT TGGTTTTGCT CAACATGCCA GGACCCCCTC GTAACCCTCA GGGTGATGAG
    AATTATATGG AGTTTCTAGA GGTGCTTACT GAGGGCTTGG AACACGTGCT GTTAGTGAGA
    GGAGGTGGAA CAGAGGTGAT CACCATTTAC TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.