Gucy2c cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gucy2c
Entrez Gene ID	110296529
Full Name	guanylate cyclase 2C
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021165238.1	XP_021020897.1	heat-stable enterotoxin receptor isoform X1
XM_021165239.1	XP_021020898.1	heat-stable enterotoxin receptor isoform X2

Pathways from BioSystems
Gene Ontology

The following Gucy2c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gucy2c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMv03241	XM_021165238.1 Latest version!	Mus caroli guanylate cyclase 2C (Gucy2c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OMv03242	XM_021165239.1 Latest version!	Mus caroli guanylate cyclase 2C (Gucy2c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gucy2c ( XM_021165238.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021020897.1 Mus caroli guanylate cyclase 2C (Gucy2c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMv03241
Clone ID Related Accession (Same CDS sequence)	XM_021165238.1
Accession Version	XM_021165238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3219bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	heat-stable enterotoxin receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034575.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCAC TGCTGGGCTT GGCTGTGCGG TTACTGCTCT TCCAGCCCGC GCTGATGATG 
TTCTGGGCCT CTCAGGTGAG GCAGAACTGC CGCAATGGCA GCTACGAGAT CAGCGTCCTG 
ATGATGGACA ACTCAGCCTA CAACGAACCT ATGCAAAACC TGAGGGAGGC TGTGGAGGAA 
GGACTGGACA TAGTGCGAAA GCGCCTGCGT GAAGCCGACC TAAATGTGAC TGTGAACGCG 
ACTTTCATCT ACTCCGACGG TCTGATTCAT AAGTCAGGTG ACTGCCGGAG CAGTACCTGT 
GAAGGCCTTG ACCTACTCAG GGAGATTACA AGAGATCGTA AGATGGGCTG CGCCCTCATG 
GGGCCCTCGT GCACGTATTC CACCTTCCAG ATGTACCTCG ACACAGAGTT GAACTATCCC 
ATGATTTCCG CTGGAAGTTA CGGGCTGTCC TGTGACTATA AGGAAACCCT AACCAGGGTC 
CTGCCTCCAG CCAGGAAGCT GATGTACTTC TTGGTCGATT TCTGGAAAGT CAACAATGCA 
TCTTTCAAAC CCTTTTCCTG GAACTCTTCG TATGTTTACA AGAATGGATC GGAGCCTGAA 
GATTGTTTCT GGTACCTCAA TGCTCTGGAG GCTGGGGTGT CCTATTTTTC TGAGGTGCTC 
AGCTTCAAGG ATGTACTGAG ACGCAGCGAA CAGTTCCAGG AAATCTTAAC AGGCCATAAC 
AGAAAGAGCA ATGTGATTGT TATGTGTGGC ACGCCAGAAA GCTTCTATGA TGTGAAAGGT 
GACCTCCAAG TGGCTGAAGA CACTGTTGTC ATCCTGGTAG ATCTGTTCAG TAACCATTAC 
TTTGAGGAGA ACACCACAGC CCCTGAGTAT ATGGACAATG TCCTCGTCCT GACGCTGCCG 
TCTGAACAGT CCACCTCAAA CGCCTCTGTC ACTGCGAGGT TTTCATCGGG GAGAAGTGAC 
TTTTCTCTCG CTTACTTGGA GGGAACCTTG CTATTTGGAC ACATGCTGCA GACGTTTCTT 
GAAAATGGAG AATCTGTCAC GGGTCCCAAG TTTGCTCGTG CATTCAGGAA TCTCACTTTT 
CAAGGCTTTG CAGGACCTGT GACTCTGGAT GACAGTGGGG ACATTGACAA CATTATGTCC 
CTTCTATATG TGTCTCTGGA TACCAGGAAA TACGAGGTTC TTATGAAGTA CGACACCCAC 
AAAAACAAAA CTATTCCGGT GGCTGACAAC CCCAACTTCA TCTGGAAGAA CCACAAGCTC 
CCCAATGACG TTCCTGGGCT GGGCCCTCAA ATCCTGATGA TTGCCGTCTT CACGCTCACG 
GGGATCCTGG TAGTTCTGCT GCTGATCGCC CTCCTCGTGC TGAGAAAATA CAGAAGAGAT 
CATGCACTTC GACAGAAGAA ATGGTCCCAC ATTCCTTCTG AAAATATCTT TCCTCTGGAG 
ACCAACGAAA CCAACCCCAT CAGCCTGAAG ATTGACGATG ACAGGAGACG AGACACAATC 
CAGAGAGTGC GACAGTGCAA ATACGACAAG AAGAAAGTGA TTCTGAAAGA CCTCAAGCAC 
AGCGACGGGA ACTTCAGTGA GAAGCAGAAG ATAGACCTGA ACAAGTTGCT GCAGTCTGAC 
TACTACAACC TGACCAAGTT CTACGGCACC GTGAAGCTGG ACACCAGGAT CTTTGGGGTG 
GTTGAGTACT GCGAGAGGGG ATCCCTCCGG GAAGTGTTAA ACGACACGAT TTCCTACCCT 
GATGGCACGT TCATGGATTG GGAGTTCAAG ATCTCTGTCT TAAATGACAT CGCTAAGGGG 
ATGTCCTACC TGCACTCCAG TAAGATTGAA GTCCACGGGC GTCTGAAATC CACCAACTGC 
GTGGTGGATA GCCGCATGGT GGTGAAGATC ACCGACTTTG GGTGCAATTC CATCCTGCCT 
CCAAAGAAAG ACCTGTGGAC GGCCCCCGAG CACCTGCGCC AGGCCACCAT CTCTCAGAAA 
GGAGATGTGT ACAGCTTCGG CATCATCGCC CAGGAGATCA TCCTCCGGAA GGAGACTTTC 
TACACGCTGA GCTGTCGGGA TCACAACGAG AAGATTTTCA GAGTGGAAAA TTCATATGGG 
AAACCTTTCC GCCCAGATCT CTTCCTGGAA ACTGCAGATG AGAAGGAGCT GGAGGTCTAT 
CTACTTGTCA AAAGCTGTTG GGAGGAGGAT CCAGAAAAGA GGCCAGATTT CAAGAAAATC 
GAGAGCACAC TGGCCAAGAT ATTTGGCCTT TTCCATGACC AGAAAAACGA GTCTTACATG 
GACACCTTGA TCCGACGTCT CCAGCTGTAC TCTCGAAACC TGGAACATCT GGTAGAGGAA 
AGGACTCAGC TGTACAAGGC AGAGAGGGAC AGGGCTGACC ACCTTAACTT CATGCTCCTC 
CCACGGCTGG TGGTAAAGTC ACTGAAGGAG AAAGGCATCG TGGAGCCAGA GCTGTATGAA 
GAAGTCACAA TCTACTTCAG TGACATTGTG GGCTTCACCA CCATCTGCAA GTACAGCACG 
CCCATGGAGG TGGTGGACAT GCTCAATGAC ATCTACAAGA GCTTTGACCA GATTGTGGAC 
CACCATGATG TCTACAAGGT AGAAACCATC GGTGACGCCT ACGTGGTGGC CAGCGGTCTG 
CCTATGAGAA ACGGCAACCG GCACGCGGTA GACATTTCCA AGATGGCCTT GGACATCCTC 
AGCTTCATAG GGACCTTTGA ATTGGAGCAT CTCCCTGGCC TCCCCGTGTG GATCCGCATT 
GGAGTTCATT CTGGCCCCTG CGCTGCTGGT GTTGTGGGGA TCAAGATGCC TCGCTATTGC 
CTGTTTGGAG ACACTGTCAA CACTGCCTCC AGGATGGAAT CCACCGGCCT CCCCTTGAGG 
ATTCACATGA GCAGCTCCAC CATAGCCATC CTGAAGAGAA CGGATTGCCA GTTCCTGTAT 
GAAGTGAGGG GAGAAACATA CTTAAAGGGA AGAGGGACAG AGACCACATA CTGGCTGACT 
GGGATGAAGG ACCAAGAATA CAACCTGCCA TCCCCACCGA CAGTGGAGAA CCAACAGCGT 
CTGCAGACTG AGTTCTCAGA CATGATCGTT AGCGCCTTAC AGAAAAGAGA GGCCTCGGGC 
AAGAAGAGCC GGAGGCCCAC TCGGGTGGCC AGCTACAAGA AAGGCTTTCT GGAATACATG 
CAGCTGAACA ATTCAGACCA CGATAGCACC TATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021020897.1
CDS	154..3372
Translation	MTSLLGLAVRLLLFQPALMMFWASQVRQNCRNGSYEISVLMMDNSAYNEPMQNLREAVEEGLDIVRKRLREADLNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDRKMGCALMGPSCTYSTFQMYLDTELNYPMISAGSYGLSCDYKETLTRVLPPARKLMYFLVDFWKVNNASFKPFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSEVLSFKDVLRRSEQFQEILTGHNRKSNVIVMCGTPESFYDVKGDLQVAEDTVVILVDLFSNHYFEENTTAPEYMDNVLVLTLPSEQSTSNASVTARFSSGRSDFSLAYLEGTLLFGHMLQTFLENGESVTGPKFARAFRNLTFQGFAGPVTLDDSGDIDNIMSLLYVSLDTRKYEVLMKYDTHKNKTIPVADNPNFIWKNHKLPNDVPGLGPQILMIAVFTLTGILVVLLLIALLVLRKYRRDHALRQKKWSHIPSENIFPLETNETNPISLKIDDDRRRDTIQRVRQCKYDKKKVILKDLKHSDGNFSEKQKIDLNKLLQSDYYNLTKFYGTVKLDTRIFGVVEYCERGSLREVLNDTISYPDGTFMDWEFKISVLNDIAKGMSYLHSSKIEVHGRLKSTNCVVDSRMVVKITDFGCNSILPPKKDLWTAPEHLRQATISQKGDVYSFGIIAQEIILRKETFYTLSCRDHNEKIFRVENSYGKPFRPDLFLETADEKELEVYLLVKSCWEEDPEKRPDFKKIESTLAKIFGLFHDQKNESYMDTLIRRLQLYSRNLEHLVEERTQLYKAERDRADHLNFMLLPRLVVKSLKEKGIVEPELYEEVTIYFSDIVGFTTICKYSTPMEVVDMLNDIYKSFDQIVDHHDVYKVETIGDAYVVASGLPMRNGNRHAVDISKMALDILSFIGTFELEHLPGLPVWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHMSSSTIAILKRTDCQFLYEVRGETYLKGRGTETTYWLTGMKDQEYNLPSPPTVENQQRLQTEFSDMIVSALQKREASGKKSRRPTRVASYKKGFLEYMQLNNSDHDSTYF

Target ORF information:

RefSeq Version	XM_021165238.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli guanylate cyclase 2C (Gucy2c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021165238.1

ORF Insert Sequence:

ATGACGTCAC TGCTGGGCTT GGCTGTGCGG TTACTGCTCT TCCAGCCCGC GCTGATGATG 
TTCTGGGCCT CTCAGGTGAG GCAGAACTGC CGCAATGGCA GCTACGAGAT CAGCGTCCTG 
ATGATGGACA ACTCAGCCTA CAACGAACCT ATGCAAAACC TGAGGGAGGC TGTGGAGGAA 
GGACTGGACA TAGTGCGAAA GCGCCTGCGT GAAGCCGACC TAAATGTGAC TGTGAACGCG 
ACTTTCATCT ACTCCGACGG TCTGATTCAT AAGTCAGGTG ACTGCCGGAG CAGTACCTGT 
GAAGGCCTTG ACCTACTCAG GGAGATTACA AGAGATCGTA AGATGGGCTG CGCCCTCATG 
GGGCCCTCGT GCACGTATTC CACCTTCCAG ATGTACCTCG ACACAGAGTT GAACTATCCC 
ATGATTTCCG CTGGAAGTTA CGGGCTGTCC TGTGACTATA AGGAAACCCT AACCAGGGTC 
CTGCCTCCAG CCAGGAAGCT GATGTACTTC TTGGTCGATT TCTGGAAAGT CAACAATGCA 
TCTTTCAAAC CCTTTTCCTG GAACTCTTCG TATGTTTACA AGAATGGATC GGAGCCTGAA 
GATTGTTTCT GGTACCTCAA TGCTCTGGAG GCTGGGGTGT CCTATTTTTC TGAGGTGCTC 
AGCTTCAAGG ATGTACTGAG ACGCAGCGAA CAGTTCCAGG AAATCTTAAC AGGCCATAAC 
AGAAAGAGCA ATGTGATTGT TATGTGTGGC ACGCCAGAAA GCTTCTATGA TGTGAAAGGT 
GACCTCCAAG TGGCTGAAGA CACTGTTGTC ATCCTGGTAG ATCTGTTCAG TAACCATTAC 
TTTGAGGAGA ACACCACAGC CCCTGAGTAT ATGGACAATG TCCTCGTCCT GACGCTGCCG 
TCTGAACAGT CCACCTCAAA CGCCTCTGTC ACTGCGAGGT TTTCATCGGG GAGAAGTGAC 
TTTTCTCTCG CTTACTTGGA GGGAACCTTG CTATTTGGAC ACATGCTGCA GACGTTTCTT 
GAAAATGGAG AATCTGTCAC GGGTCCCAAG TTTGCTCGTG CATTCAGGAA TCTCACTTTT 
CAAGGCTTTG CAGGACCTGT GACTCTGGAT GACAGTGGGG ACATTGACAA CATTATGTCC 
CTTCTATATG TGTCTCTGGA TACCAGGAAA TACGAGGTTC TTATGAAGTA CGACACCCAC 
AAAAACAAAA CTATTCCGGT GGCTGACAAC CCCAACTTCA TCTGGAAGAA CCACAAGCTC 
CCCAATGACG TTCCTGGGCT GGGCCCTCAA ATCCTGATGA TTGCCGTCTT CACGCTCACG 
GGGATCCTGG TAGTTCTGCT GCTGATCGCC CTCCTCGTGC TGAGAAAATA CAGAAGAGAT 
CATGCACTTC GACAGAAGAA ATGGTCCCAC ATTCCTTCTG AAAATATCTT TCCTCTGGAG 
ACCAACGAAA CCAACCCCAT CAGCCTGAAG ATTGACGATG ACAGGAGACG AGACACAATC 
CAGAGAGTGC GACAGTGCAA ATACGACAAG AAGAAAGTGA TTCTGAAAGA CCTCAAGCAC 
AGCGACGGGA ACTTCAGTGA GAAGCAGAAG ATAGACCTGA ACAAGTTGCT GCAGTCTGAC 
TACTACAACC TGACCAAGTT CTACGGCACC GTGAAGCTGG ACACCAGGAT CTTTGGGGTG 
GTTGAGTACT GCGAGAGGGG ATCCCTCCGG GAAGTGTTAA ACGACACGAT TTCCTACCCT 
GATGGCACGT TCATGGATTG GGAGTTCAAG ATCTCTGTCT TAAATGACAT CGCTAAGGGG 
ATGTCCTACC TGCACTCCAG TAAGATTGAA GTCCACGGGC GTCTGAAATC CACCAACTGC 
GTGGTGGATA GCCGCATGGT GGTGAAGATC ACCGACTTTG GGTGCAATTC CATCCTGCCT 
CCAAAGAAAG ACCTGTGGAC GGCCCCCGAG CACCTGCGCC AGGCCACCAT CTCTCAGAAA 
GGAGATGTGT ACAGCTTCGG CATCATCGCC CAGGAGATCA TCCTCCGGAA GGAGACTTTC 
TACACGCTGA GCTGTCGGGA TCACAACGAG AAGATTTTCA GAGTGGAAAA TTCATATGGG 
AAACCTTTCC GCCCAGATCT CTTCCTGGAA ACTGCAGATG AGAAGGAGCT GGAGGTCTAT 
CTACTTGTCA AAAGCTGTTG GGAGGAGGAT CCAGAAAAGA GGCCAGATTT CAAGAAAATC 
GAGAGCACAC TGGCCAAGAT ATTTGGCCTT TTCCATGACC AGAAAAACGA GTCTTACATG 
GACACCTTGA TCCGACGTCT CCAGCTGTAC TCTCGAAACC TGGAACATCT GGTAGAGGAA 
AGGACTCAGC TGTACAAGGC AGAGAGGGAC AGGGCTGACC ACCTTAACTT CATGCTCCTC 
CCACGGCTGG TGGTAAAGTC ACTGAAGGAG AAAGGCATCG TGGAGCCAGA GCTGTATGAA 
GAAGTCACAA TCTACTTCAG TGACATTGTG GGCTTCACCA CCATCTGCAA GTACAGCACG 
CCCATGGAGG TGGTGGACAT GCTCAATGAC ATCTACAAGA GCTTTGACCA GATTGTGGAC 
CACCATGATG TCTACAAGGT AGAAACCATC GGTGACGCCT ACGTGGTGGC CAGCGGTCTG 
CCTATGAGAA ACGGCAACCG GCACGCGGTA GACATTTCCA AGATGGCCTT GGACATCCTC 
AGCTTCATAG GGACCTTTGA ATTGGAGCAT CTCCCTGGCC TCCCCGTGTG GATCCGCATT 
GGAGTTCATT CTGGCCCCTG CGCTGCTGGT GTTGTGGGGA TCAAGATGCC TCGCTATTGC 
CTGTTTGGAG ACACTGTCAA CACTGCCTCC AGGATGGAAT CCACCGGCCT CCCCTTGAGG 
ATTCACATGA GCAGCTCCAC CATAGCCATC CTGAAGAGAA CGGATTGCCA GTTCCTGTAT 
GAAGTGAGGG GAGAAACATA CTTAAAGGGA AGAGGGACAG AGACCACATA CTGGCTGACT 
GGGATGAAGG ACCAAGAATA CAACCTGCCA TCCCCACCGA CAGTGGAGAA CCAACAGCGT 
CTGCAGACTG AGTTCTCAGA CATGATCGTT AGCGCCTTAC AGAAAAGAGA GGCCTCGGGC 
AAGAAGAGCC GGAGGCCCAC TCGGGTGGCC AGCTACAAGA AAGGCTTTCT GGAATACATG 
CAGCTGAACA ATTCAGACCA CGATAGCACC TATTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gucy2c ( XM_021165239.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021020898.1 Mus caroli guanylate cyclase 2C (Gucy2c), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMv03242
Clone ID Related Accession (Same CDS sequence)	XM_021165239.1
Accession Version	XM_021165239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	heat-stable enterotoxin receptor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034575.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGTCAC TGCTGGGCTT GGCTGTGCGG TTACTGCTCT TCCAGCCCGC GCTGATGATG 
TTCTGGGCCT CTCAGGTGAG GCAGAACTGC CGCAATGGCA GCTACGAGAT CAGCGTCCTG 
ATGATGGACA ACTCAGCCTA CAACGAACCT ATGCAAAACC TGAGGGAGGC TGTGGAGGAA 
GGACTGGACA TAGTGCGAAA GCGCCTGCGT GAAGCCGACC TAAATGTGAC TGTGAACGCG 
ACTTTCATCT ACTCCGACGG TCTGATTCAT AAGTCAGGTG ACTGCCGGAG CAGTACCTGT 
GAAGGCCTTG ACCTACTCAG GGAGATTACA AGAGATCGTA AGATGGGCTG CGCCCTCATG 
GGGCCCTCGT GCACGTATTC CACCTTCCAG ATGTACCTCG ACACAGAGTT GAACTATCCC 
ATGATTTCCG CTGGAAGTTA CGGGCTGTCC TGTGACTATA AGGAAACCCT AACCAGGGTC 
CTGCCTCCAG CCAGGAAGCT GATGTACTTC TTGGTCGATT TCTGGAAAGT CAACAATGCA 
TCTTTCAAAC CCTTTTCCTG GAACTCTTCG TATGTTTACA AGAATGGATC GGAGCCTGAA 
GATTGTTTCT GGTACCTCAA TGCTCTGGAG GCTGGGGTGT CCTATTTTTC TGAGGTGCTC 
AGCTTCAAGG ATGTACTGAG ACGCAGCGAA CAGTTCCAGG AAATCTTAAC AGGCCATAAC 
AGAAAGAGCA ATGTGATTGT TATGTGTGGC ACGCCAGAAA GCTTCTATGA TGTGAAAGGT 
GACCTCCAAG TGGCTGAAGA CACTGTTGTC ATCCTGGTAG ATCTGTTCAG TAACCATTAC 
TTTGAGGAGA ACACCACAGC CCCTGAGTAT ATGGACAATG TCCTCGTCCT GACGCTGCCG 
TCTGAACAGT CCACCTCAAA CGCCTCTGTC ACTGCGAGGT TTTCATCGGG GAGAAGTGAC 
TTTTCTCTCG CTTACTTGGA GGGAACCTTG CTATTTGGAC ACATGCTGCA GACGTTTCTT 
GAAAATGGAG AATCTGTCAC GGGTCCCAAG TTTGCTCGTG CATTCAGGAA TCTCACTTTT 
CAAGGCTTTG CAGGACCTGT GACTCTGGAT GACAGTGGGG ACATTGACAA CATTATGTCC 
CTTCTATATG TGTCTCTGGA TACCAGGAAA TACGAGGTTC TTATGAAGTA CGACACCCAC 
AAAAACAAAA CTATTCCGGT GGCTGACAAC CCCAACTTCA TCTGGAAGAA CCACAAGCTC 
CCCAATGACG TTCCTGGGCT GGGCCCTCAA ATCCTGATGA TTGCCGTCTT CACGCTCACG 
GGGATCCTGG TAGTTCTGCT GCTGATCGCC CTCCTCGTGC TGAGAAAATA CAGAAGAGAT 
CATGCACTTC GACAGAAGAA ATGGTCCCAC ATTCCTTCTG AAAATATCTT TCCTCTGGAG 
ACCAACGAAA CCAACCCCAT CAGCCTGAAG ATTGACGATG ACAGGAGACG AGACACAATC 
CAGAGAGTGC GACAGTGCAA ATACGACAAG AAGTTGCTGC AGTCTGACTA CTACAACCTG 
ACCAAGTTCT ACGGCACCGT GAAGCTGGAC ACCAGGATCT TTGGGGTGGT TGAGTACTGC 
GAGAGGGGAT CCCTCCGGGA AGTGTTAAAC GACACGATTT CCTACCCTGA TGGCACGTTC 
ATGGATTGGG AGTTCAAGAT CTCTGTCTTA AATGACATCG CTAAGGGGAT GTCCTACCTG 
CACTCCAGTA AGATTGAAGT CCACGGGCGT CTGAAATCCA CCAACTGCGT GGTGGATAGC 
CGCATGGTGG TGAAGATCAC CGACTTTGGG TGCAATTCCA TCCTGCCTCC AAAGAAAGAC 
CTGTGGACGG CCCCCGAGCA CCTGCGCCAG GCCACCATCT CTCAGAAAGG AGATGTGTAC 
AGCTTCGGCA TCATCGCCCA GGAGATCATC CTCCGGAAGG AGACTTTCTA CACGCTGAGC 
TGTCGGGATC ACAACGAGAA GATTTTCAGA GTGGAAAATT CATATGGGAA ACCTTTCCGC 
CCAGATCTCT TCCTGGAAAC TGCAGATGAG AAGGAGCTGG AGGTCTATCT ACTTGTCAAA 
AGCTGTTGGG AGGAGGATCC AGAAAAGAGG CCAGATTTCA AGAAAATCGA GAGCACACTG 
GCCAAGATAT TTGGCCTTTT CCATGACCAG AAAAACGAGT CTTACATGGA CACCTTGATC 
CGACGTCTCC AGCTGTACTC TCGAAACCTG GAACATCTGG TAGAGGAAAG GACTCAGCTG 
TACAAGGCAG AGAGGGACAG GGCTGACCAC CTTAACTTCA TGCTCCTCCC ACGGCTGGTG 
GTAAAGTCAC TGAAGGAGAA AGGCATCGTG GAGCCAGAGC TGTATGAAGA AGTCACAATC 
TACTTCAGTG ACATTGTGGG CTTCACCACC ATCTGCAAGT ACAGCACGCC CATGGAGGTG 
GTGGACATGC TCAATGACAT CTACAAGAGC TTTGACCAGA TTGTGGACCA CCATGATGTC 
TACAAGGTAG AAACCATCGG TGACGCCTAC GTGGTGGCCA GCGGTCTGCC TATGAGAAAC 
GGCAACCGGC ACGCGGTAGA CATTTCCAAG ATGGCCTTGG ACATCCTCAG CTTCATAGGG 
ACCTTTGAAT TGGAGCATCT CCCTGGCCTC CCCGTGTGGA TCCGCATTGG AGTTCATTCT 
GGCCCCTGCG CTGCTGGTGT TGTGGGGATC AAGATGCCTC GCTATTGCCT GTTTGGAGAC 
ACTGTCAACA CTGCCTCCAG GATGGAATCC ACCGGCCTCC CCTTGAGGAT TCACATGAGC 
AGCTCCACCA TAGCCATCCT GAAGAGAACG GATTGCCAGT TCCTGTATGA AGTGAGGGGA 
GAAACATACT TAAAGGGAAG AGGGACAGAG ACCACATACT GGCTGACTGG GATGAAGGAC 
CAAGAATACA ACCTGCCATC CCCACCGACA GTGGAGAACC AACAGCGTCT GCAGACTGAG 
TTCTCAGACA TGATCGTTAG CGCCTTACAG AAAAGAGAGG CCTCGGGCAA GAAGAGCCGG 
AGGCCCACTC GGGTGGCCAG CTACAAGAAA GGCTTTCTGG AATACATGCA GCTGAACAAT 
TCAGACCACG ATAGCACCTA TTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021020898.1
CDS	131..3277
Translation	MTSLLGLAVRLLLFQPALMMFWASQVRQNCRNGSYEISVLMMDNSAYNEPMQNLREAVEEGLDIVRKRLREADLNVTVNATFIYSDGLIHKSGDCRSSTCEGLDLLREITRDRKMGCALMGPSCTYSTFQMYLDTELNYPMISAGSYGLSCDYKETLTRVLPPARKLMYFLVDFWKVNNASFKPFSWNSSYVYKNGSEPEDCFWYLNALEAGVSYFSEVLSFKDVLRRSEQFQEILTGHNRKSNVIVMCGTPESFYDVKGDLQVAEDTVVILVDLFSNHYFEENTTAPEYMDNVLVLTLPSEQSTSNASVTARFSSGRSDFSLAYLEGTLLFGHMLQTFLENGESVTGPKFARAFRNLTFQGFAGPVTLDDSGDIDNIMSLLYVSLDTRKYEVLMKYDTHKNKTIPVADNPNFIWKNHKLPNDVPGLGPQILMIAVFTLTGILVVLLLIALLVLRKYRRDHALRQKKWSHIPSENIFPLETNETNPISLKIDDDRRRDTIQRVRQCKYDKKLLQSDYYNLTKFYGTVKLDTRIFGVVEYCERGSLREVLNDTISYPDGTFMDWEFKISVLNDIAKGMSYLHSSKIEVHGRLKSTNCVVDSRMVVKITDFGCNSILPPKKDLWTAPEHLRQATISQKGDVYSFGIIAQEIILRKETFYTLSCRDHNEKIFRVENSYGKPFRPDLFLETADEKELEVYLLVKSCWEEDPEKRPDFKKIESTLAKIFGLFHDQKNESYMDTLIRRLQLYSRNLEHLVEERTQLYKAERDRADHLNFMLLPRLVVKSLKEKGIVEPELYEEVTIYFSDIVGFTTICKYSTPMEVVDMLNDIYKSFDQIVDHHDVYKVETIGDAYVVASGLPMRNGNRHAVDISKMALDILSFIGTFELEHLPGLPVWIRIGVHSGPCAAGVVGIKMPRYCLFGDTVNTASRMESTGLPLRIHMSSSTIAILKRTDCQFLYEVRGETYLKGRGTETTYWLTGMKDQEYNLPSPPTVENQQRLQTEFSDMIVSALQKREASGKKSRRPTRVASYKKGFLEYMQLNNSDHDSTYF

Target ORF information:

RefSeq Version	XM_021165239.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli guanylate cyclase 2C (Gucy2c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021165239.1

ORF Insert Sequence:

ATGACGTCAC TGCTGGGCTT GGCTGTGCGG TTACTGCTCT TCCAGCCCGC GCTGATGATG 
TTCTGGGCCT CTCAGGTGAG GCAGAACTGC CGCAATGGCA GCTACGAGAT CAGCGTCCTG 
ATGATGGACA ACTCAGCCTA CAACGAACCT ATGCAAAACC TGAGGGAGGC TGTGGAGGAA 
GGACTGGACA TAGTGCGAAA GCGCCTGCGT GAAGCCGACC TAAATGTGAC TGTGAACGCG 
ACTTTCATCT ACTCCGACGG TCTGATTCAT AAGTCAGGTG ACTGCCGGAG CAGTACCTGT 
GAAGGCCTTG ACCTACTCAG GGAGATTACA AGAGATCGTA AGATGGGCTG CGCCCTCATG 
GGGCCCTCGT GCACGTATTC CACCTTCCAG ATGTACCTCG ACACAGAGTT GAACTATCCC 
ATGATTTCCG CTGGAAGTTA CGGGCTGTCC TGTGACTATA AGGAAACCCT AACCAGGGTC 
CTGCCTCCAG CCAGGAAGCT GATGTACTTC TTGGTCGATT TCTGGAAAGT CAACAATGCA 
TCTTTCAAAC CCTTTTCCTG GAACTCTTCG TATGTTTACA AGAATGGATC GGAGCCTGAA 
GATTGTTTCT GGTACCTCAA TGCTCTGGAG GCTGGGGTGT CCTATTTTTC TGAGGTGCTC 
AGCTTCAAGG ATGTACTGAG ACGCAGCGAA CAGTTCCAGG AAATCTTAAC AGGCCATAAC 
AGAAAGAGCA ATGTGATTGT TATGTGTGGC ACGCCAGAAA GCTTCTATGA TGTGAAAGGT 
GACCTCCAAG TGGCTGAAGA CACTGTTGTC ATCCTGGTAG ATCTGTTCAG TAACCATTAC 
TTTGAGGAGA ACACCACAGC CCCTGAGTAT ATGGACAATG TCCTCGTCCT GACGCTGCCG 
TCTGAACAGT CCACCTCAAA CGCCTCTGTC ACTGCGAGGT TTTCATCGGG GAGAAGTGAC 
TTTTCTCTCG CTTACTTGGA GGGAACCTTG CTATTTGGAC ACATGCTGCA GACGTTTCTT 
GAAAATGGAG AATCTGTCAC GGGTCCCAAG TTTGCTCGTG CATTCAGGAA TCTCACTTTT 
CAAGGCTTTG CAGGACCTGT GACTCTGGAT GACAGTGGGG ACATTGACAA CATTATGTCC 
CTTCTATATG TGTCTCTGGA TACCAGGAAA TACGAGGTTC TTATGAAGTA CGACACCCAC 
AAAAACAAAA CTATTCCGGT GGCTGACAAC CCCAACTTCA TCTGGAAGAA CCACAAGCTC 
CCCAATGACG TTCCTGGGCT GGGCCCTCAA ATCCTGATGA TTGCCGTCTT CACGCTCACG 
GGGATCCTGG TAGTTCTGCT GCTGATCGCC CTCCTCGTGC TGAGAAAATA CAGAAGAGAT 
CATGCACTTC GACAGAAGAA ATGGTCCCAC ATTCCTTCTG AAAATATCTT TCCTCTGGAG 
ACCAACGAAA CCAACCCCAT CAGCCTGAAG ATTGACGATG ACAGGAGACG AGACACAATC 
CAGAGAGTGC GACAGTGCAA ATACGACAAG AAGTTGCTGC AGTCTGACTA CTACAACCTG 
ACCAAGTTCT ACGGCACCGT GAAGCTGGAC ACCAGGATCT TTGGGGTGGT TGAGTACTGC 
GAGAGGGGAT CCCTCCGGGA AGTGTTAAAC GACACGATTT CCTACCCTGA TGGCACGTTC 
ATGGATTGGG AGTTCAAGAT CTCTGTCTTA AATGACATCG CTAAGGGGAT GTCCTACCTG 
CACTCCAGTA AGATTGAAGT CCACGGGCGT CTGAAATCCA CCAACTGCGT GGTGGATAGC 
CGCATGGTGG TGAAGATCAC CGACTTTGGG TGCAATTCCA TCCTGCCTCC AAAGAAAGAC 
CTGTGGACGG CCCCCGAGCA CCTGCGCCAG GCCACCATCT CTCAGAAAGG AGATGTGTAC 
AGCTTCGGCA TCATCGCCCA GGAGATCATC CTCCGGAAGG AGACTTTCTA CACGCTGAGC 
TGTCGGGATC ACAACGAGAA GATTTTCAGA GTGGAAAATT CATATGGGAA ACCTTTCCGC 
CCAGATCTCT TCCTGGAAAC TGCAGATGAG AAGGAGCTGG AGGTCTATCT ACTTGTCAAA 
AGCTGTTGGG AGGAGGATCC AGAAAAGAGG CCAGATTTCA AGAAAATCGA GAGCACACTG 
GCCAAGATAT TTGGCCTTTT CCATGACCAG AAAAACGAGT CTTACATGGA CACCTTGATC 
CGACGTCTCC AGCTGTACTC TCGAAACCTG GAACATCTGG TAGAGGAAAG GACTCAGCTG 
TACAAGGCAG AGAGGGACAG GGCTGACCAC CTTAACTTCA TGCTCCTCCC ACGGCTGGTG 
GTAAAGTCAC TGAAGGAGAA AGGCATCGTG GAGCCAGAGC TGTATGAAGA AGTCACAATC 
TACTTCAGTG ACATTGTGGG CTTCACCACC ATCTGCAAGT ACAGCACGCC CATGGAGGTG 
GTGGACATGC TCAATGACAT CTACAAGAGC TTTGACCAGA TTGTGGACCA CCATGATGTC 
TACAAGGTAG AAACCATCGG TGACGCCTAC GTGGTGGCCA GCGGTCTGCC TATGAGAAAC 
GGCAACCGGC ACGCGGTAGA CATTTCCAAG ATGGCCTTGG ACATCCTCAG CTTCATAGGG 
ACCTTTGAAT TGGAGCATCT CCCTGGCCTC CCCGTGTGGA TCCGCATTGG AGTTCATTCT 
GGCCCCTGCG CTGCTGGTGT TGTGGGGATC AAGATGCCTC GCTATTGCCT GTTTGGAGAC 
ACTGTCAACA CTGCCTCCAG GATGGAATCC ACCGGCCTCC CCTTGAGGAT TCACATGAGC 
AGCTCCACCA TAGCCATCCT GAAGAGAACG GATTGCCAGT TCCTGTATGA AGTGAGGGGA 
GAAACATACT TAAAGGGAAG AGGGACAGAG ACCACATACT GGCTGACTGG GATGAAGGAC 
CAAGAATACA ACCTGCCATC CCCACCGACA GTGGAGAACC AACAGCGTCT GCAGACTGAG 
TTCTCAGACA TGATCGTTAG CGCCTTACAG AAAAGAGAGG CCTCGGGCAA GAAGAGCCGG 
AGGCCCACTC GGGTGGCCAG CTACAAGAAA GGCTTTCTGG AATACATGCA GCTGAACAAT 
TCAGACCACG ATAGCACCTA TTTTTAG