Aars2 cDNA ORF clone, Mus caroli(Ryukyu mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aars2
Entrez Gene ID	110284096
Full Name	alanyl-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Mus caroli(Ryukyu mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021149720.2	XP_021005379.1	alanine--tRNA ligase, mitochondrial
XM_021149720.1	XP_021005379.1	alanine--tRNA ligase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following Aars2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aars2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMv35100	XM_021149720.2 Latest version!	Mus caroli alanyl-tRNA synthetase 2, mitochondrial (Aars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OMv35100	XM_021149720.1	Mus caroli alanyl-tRNA synthetase 2, mitochondrial (Aars2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aars2 ( XM_021149720.2 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021005379.1 Mus caroli alanyl-tRNA synthetase 2, mitochondrial (Aars2), mRNA.

Price & Availability↑

CloneID	OMv35100
Clone ID Related Accession (Same CDS sequence)	XM_021149720.2 , XM_021149720.1
Accession Version	XM_021149720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-09
Organism	Mus caroli(Ryukyu mouse)
Product	alanine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034586.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 10, 2019 this sequence version replaced XM_021149720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus caroli Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG CGTTGGCTGC CGCAGCCGGT AAGCTGCGGC GAGCCATTGG GAGGTCGTGC 
CCATGGCAGC CTTTCTCAAC CGAACCCGGT CCACCCCACG GAGCGGCCGT GCGGGACGCC 
TTCCTGAGCT TCTTCCGAGA TCGCCACGGC CACCGGCTCG TGCCCTCCGC TACCGTGAGG 
CCGCGCGGCG ACCCCAGCCT GCTTTTCGTC AATGCAGGCA TGAACCAGTT CAAGCCCATC 
TTCCTGGGCA CAGTGGATCC ACGAAGCGAG ATGGCAGGCT TCCGACGTGT TGCTAATAGC 
CAGAAGTGTG TCAGGGCTGG AGGACGCCAT AACGACCTGG AGGATGTGGG CCGTGATCTC 
TCTCATCATA CGTTTTTCGA GATGCTTGGC AACTGGGCTT TCGGGGGTGA ATATTTTAAG 
GAGGAAGCTT GTAGCATGGC CTGGGAACTT CTGACTCAAG TCTACGGGAT CCCTGAGGAC 
AGGTTGTGGG TCTCCTACTT CAGTGGTGAC TCCCAGACAG GACTGGACCC AGACCTGGAG 
ACCAGAGACA TCTGGCTAAG CTTGGGAGTG CCTGCCAGCC GTGTGCTCTC CTTCGGACCA 
CAAGAGAACT TCTGGGAGAT GGGAGACACT GGGCCTTGTG GGCCTTGTAC TGAGATCCAC 
TATGACCTGG CTGGGGACGT GGGAAGCCCC CAGCTGGTAG AGCTTTGGAA TCTGGTCTTC 
ATGCAACATT ACAGAGAGGC AGATGGAAGC CTGCAGCTGC TGCCGCAGCG ACATGTAGAC 
ACAGGAATGG GCCTGGAAAG GCTGGTGGCC ATCCTGCAGG GCAAACGTTC CACCTACGAC 
ACTGACCTCT TCTCTCCACT GCTCGACGCC ATACACCAGA GCTGCGGGGC TCCCCCTTAC 
TCTGGCCGGG TAGGGGCAGC AGATGAGGGC CGGATAGACA CAGCGTACCG AGTGGTAGCC 
GATCACATCC GCACACTCAG TGTCTGCATT GCTGACGGTG TCTCCCCGGG GATGTCAGGT 
GCCCCGCTCG TTCTCCGTCG GATTCTCCGC CGAGCTGTGC GCTATTCCAC AGAGGTCTTG 
CAGGCACCAC CTGGTTTTCT AGGCAGCCTG GTGCCAGTGG TGGTAGAGAC ACTGGGATCT 
GCTTATCCCG AACTTGAGAA GAACTCAGTC AAGATAGCCA GCCTGGTGTC AGAGGATGAG 
GCAGCCTTCC TGGCCTCCCT GCAGCGAGGC CGGCGGATCA TCGATCGCAC CGTCAAGCGC 
CTGGGCCCTT CTGATTTGTT CCCTGCCGAA GTGGCCTGGT CATTGTCGCT GTCTGGGAAT 
CTGGGGATTC CCCTGGACCT GGTGGAGCTG ATGCTGGAGG AGAAGGAGGT GAAGCTGGAC 
ACAGCAGGAG TGGAGCAGCT AGCCAAGAAG GAGGCTCAGC ACCGGGCCCA GCAGGCAGAG 
GCAGATCAGG AGGACCGATT GTGTCTTGAT GTCCACGCAC TGGAGGAGCT GCACCGTCAA 
GGCATACCCC CAACTGATGA CAGCCCCAAG TATAACTATT CTCTTCACCC CAACGGGGAT 
TACGAGTTTG GCCTCTGTGA GGCCCGGGTG TTACAGCTGT ATTCAGAGAC TGGGACGGCC 
GTGGCCTCCG TGGGAGCAGG CCAGCGCTGT GGCCTCCTAT TAGACAGAAC CAACTTCTAC 
GCTGAACAAG GGGGTCAGGC TTCAGACCGA GGCTACCTTG TCCGTACAGG GCAGCAGGAT 
GTGTTGTTCC CTGTGGCCGG GGCTCAGCTC TGTGGGGGTT TTATCCTGCA TGAGGCCATG 
GCTCCCGAGC ACCTACAGGT GGGGGATCAA GTGCAGCTGT ATGTAGATAC GGCCTGGCGA 
ATGGGTTGCA TGGTGAAGCA CACGGCCACC CACCTGCTGA GCTGGGCCCT TCGGCAGACC 
CTCGGACCGA CCACCGAGCA GCGGGGCTCC CATCTCAACC CCGAGCGGCT GCGCTTTGAC 
GTGGCCACCC AGACCCCACT GACCACAGAG CAGCTACGGA CGGTAGAGAG CTACGTGCAG 
GAAGTCGTGG GGCAGGATAA GCCTGTGTTC ATGGAGGAAG TGCCCCTGGC ACACACTGCC 
CGCATCCCCG GCCTTCGCTC ACTGGATGAG GTGTACCCAG ACCCCGTCCG GGTGGTGTCC 
GTTGGGGTTC CTGTTGCCCA CGCACTGGGG CCAGCCTCCC AGGCTGCAAT GCACACCTCC 
GTGGAGCTGT GCTGTGGGAC GCACCTGCTA AGTACTGGGG CCGTGGGGGA CTTGGTGATC 
ATTGGGGATC GGCAGCTGGT CAAGGGCATC ACTCGCCTAC TGGCCATTAC TGGGGAGCAG 
GCCCAACAGG CCCGAGAGGT AGGCCAGAGC CTGTCTCAGG AGGTGGAAGC AGCCAGCGAG 
CGACTAAGTC GGGGCAGCCG AGACCTGCCA GAAGCTCACC GGCTATCTAA GGACATAGGA 
CGTCTCACCG AGGTAGTAGA GTCTGCTGTG ATACCTCAGT GGCAACGGCA GGAGCTGCAG 
ACCACACTAA AGATGCTACA GCGCCGTGCC AACACTGCCA TCCGGAAACT GGAGAAGGGC 
CAGGCTACAG AGAAATCCCA GGAGCTGTTG AAACGACACT CGGAGGGGCC TCTGATTGTG 
GACACTGTCT CTGCCGAGTC CCTCTCAGTG CTGGTAAAGG TCGTGCGGCA GCTGTGCAAA 
CAGGCCCCCA GCATATCCGT GCTGCTGCTC AGCCCCCAGC CCACTGGCAG TGTCCTGTGT 
GCCTGCCAGG TGGCCCAGGG TGCCACGCCC ACCTTCACAG CTGAAGCCTG GGCACTGGCT 
GTGTGCAGCC ACATGGGAGG CAAGGCATGG GGCTCCAGAG TGGTAGCTCA GGGGACTGGA 
CACACGGCTG ACCTGGAGGC CGCCCTCGGG ACAGCCCGAG CTTACGCACT CAGCCAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021005379.1
CDS	30..2972
Translation	MAVALAAAAGKLRRAIGRSCPWQPFSTEPGPPHGAAVRDAFLSFFRDRHGHRLVPSATVRPRGDPSLLFVNAGMNQFKPIFLGTVDPRSEMAGFRRVANSQKCVRAGGRHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFKEEACSMAWELLTQVYGIPEDRLWVSYFSGDSQTGLDPDLETRDIWLSLGVPASRVLSFGPQENFWEMGDTGPCGPCTEIHYDLAGDVGSPQLVELWNLVFMQHYREADGSLQLLPQRHVDTGMGLERLVAILQGKRSTYDTDLFSPLLDAIHQSCGAPPYSGRVGAADEGRIDTAYRVVADHIRTLSVCIADGVSPGMSGAPLVLRRILRRAVRYSTEVLQAPPGFLGSLVPVVVETLGSAYPELEKNSVKIASLVSEDEAAFLASLQRGRRIIDRTVKRLGPSDLFPAEVAWSLSLSGNLGIPLDLVELMLEEKEVKLDTAGVEQLAKKEAQHRAQQAEADQEDRLCLDVHALEELHRQGIPPTDDSPKYNYSLHPNGDYEFGLCEARVLQLYSETGTAVASVGAGQRCGLLLDRTNFYAEQGGQASDRGYLVRTGQQDVLFPVAGAQLCGGFILHEAMAPEHLQVGDQVQLYVDTAWRMGCMVKHTATHLLSWALRQTLGPTTEQRGSHLNPERLRFDVATQTPLTTEQLRTVESYVQEVVGQDKPVFMEEVPLAHTARIPGLRSLDEVYPDPVRVVSVGVPVAHALGPASQAAMHTSVELCCGTHLLSTGAVGDLVIIGDRQLVKGITRLLAITGEQAQQAREVGQSLSQEVEAASERLSRGSRDLPEAHRLSKDIGRLTEVVESAVIPQWQRQELQTTLKMLQRRANTAIRKLEKGQATEKSQELLKRHSEGPLIVDTVSAESLSVLVKVVRQLCKQAPSISVLLLSPQPTGSVLCACQVAQGATPTFTAEAWALAVCSHMGGKAWGSRVVAQGTGHTADLEAALGTARAYALSQL

Target ORF information:

RefSeq Version	XM_021149720.2
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli alanyl-tRNA synthetase 2, mitochondrial (Aars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021149720.2

ORF Insert Sequence:

ATGGCGGTGG CGTTGGCTGC CGCAGCCGGT AAGCTGCGGC GAGCCATTGG GAGGTCGTGC 
CCATGGCAGC CTTTCTCAAC CGAACCCGGT CCACCCCACG GAGCGGCCGT GCGGGACGCC 
TTCCTGAGCT TCTTCCGAGA TCGCCACGGC CACCGGCTCG TGCCCTCCGC TACCGTGAGG 
CCGCGCGGCG ACCCCAGCCT GCTTTTCGTC AATGCAGGCA TGAACCAGTT CAAGCCCATC 
TTCCTGGGCA CAGTGGATCC ACGAAGCGAG ATGGCAGGCT TCCGACGTGT TGCTAATAGC 
CAGAAGTGTG TCAGGGCTGG AGGACGCCAT AACGACCTGG AGGATGTGGG CCGTGATCTC 
TCTCATCATA CGTTTTTCGA GATGCTTGGC AACTGGGCTT TCGGGGGTGA ATATTTTAAG 
GAGGAAGCTT GTAGCATGGC CTGGGAACTT CTGACTCAAG TCTACGGGAT CCCTGAGGAC 
AGGTTGTGGG TCTCCTACTT CAGTGGTGAC TCCCAGACAG GACTGGACCC AGACCTGGAG 
ACCAGAGACA TCTGGCTAAG CTTGGGAGTG CCTGCCAGCC GTGTGCTCTC CTTCGGACCA 
CAAGAGAACT TCTGGGAGAT GGGAGACACT GGGCCTTGTG GGCCTTGTAC TGAGATCCAC 
TATGACCTGG CTGGGGACGT GGGAAGCCCC CAGCTGGTAG AGCTTTGGAA TCTGGTCTTC 
ATGCAACATT ACAGAGAGGC AGATGGAAGC CTGCAGCTGC TGCCGCAGCG ACATGTAGAC 
ACAGGAATGG GCCTGGAAAG GCTGGTGGCC ATCCTGCAGG GCAAACGTTC CACCTACGAC 
ACTGACCTCT TCTCTCCACT GCTCGACGCC ATACACCAGA GCTGCGGGGC TCCCCCTTAC 
TCTGGCCGGG TAGGGGCAGC AGATGAGGGC CGGATAGACA CAGCGTACCG AGTGGTAGCC 
GATCACATCC GCACACTCAG TGTCTGCATT GCTGACGGTG TCTCCCCGGG GATGTCAGGT 
GCCCCGCTCG TTCTCCGTCG GATTCTCCGC CGAGCTGTGC GCTATTCCAC AGAGGTCTTG 
CAGGCACCAC CTGGTTTTCT AGGCAGCCTG GTGCCAGTGG TGGTAGAGAC ACTGGGATCT 
GCTTATCCCG AACTTGAGAA GAACTCAGTC AAGATAGCCA GCCTGGTGTC AGAGGATGAG 
GCAGCCTTCC TGGCCTCCCT GCAGCGAGGC CGGCGGATCA TCGATCGCAC CGTCAAGCGC 
CTGGGCCCTT CTGATTTGTT CCCTGCCGAA GTGGCCTGGT CATTGTCGCT GTCTGGGAAT 
CTGGGGATTC CCCTGGACCT GGTGGAGCTG ATGCTGGAGG AGAAGGAGGT GAAGCTGGAC 
ACAGCAGGAG TGGAGCAGCT AGCCAAGAAG GAGGCTCAGC ACCGGGCCCA GCAGGCAGAG 
GCAGATCAGG AGGACCGATT GTGTCTTGAT GTCCACGCAC TGGAGGAGCT GCACCGTCAA 
GGCATACCCC CAACTGATGA CAGCCCCAAG TATAACTATT CTCTTCACCC CAACGGGGAT 
TACGAGTTTG GCCTCTGTGA GGCCCGGGTG TTACAGCTGT ATTCAGAGAC TGGGACGGCC 
GTGGCCTCCG TGGGAGCAGG CCAGCGCTGT GGCCTCCTAT TAGACAGAAC CAACTTCTAC 
GCTGAACAAG GGGGTCAGGC TTCAGACCGA GGCTACCTTG TCCGTACAGG GCAGCAGGAT 
GTGTTGTTCC CTGTGGCCGG GGCTCAGCTC TGTGGGGGTT TTATCCTGCA TGAGGCCATG 
GCTCCCGAGC ACCTACAGGT GGGGGATCAA GTGCAGCTGT ATGTAGATAC GGCCTGGCGA 
ATGGGTTGCA TGGTGAAGCA CACGGCCACC CACCTGCTGA GCTGGGCCCT TCGGCAGACC 
CTCGGACCGA CCACCGAGCA GCGGGGCTCC CATCTCAACC CCGAGCGGCT GCGCTTTGAC 
GTGGCCACCC AGACCCCACT GACCACAGAG CAGCTACGGA CGGTAGAGAG CTACGTGCAG 
GAAGTCGTGG GGCAGGATAA GCCTGTGTTC ATGGAGGAAG TGCCCCTGGC ACACACTGCC 
CGCATCCCCG GCCTTCGCTC ACTGGATGAG GTGTACCCAG ACCCCGTCCG GGTGGTGTCC 
GTTGGGGTTC CTGTTGCCCA CGCACTGGGG CCAGCCTCCC AGGCTGCAAT GCACACCTCC 
GTGGAGCTGT GCTGTGGGAC GCACCTGCTA AGTACTGGGG CCGTGGGGGA CTTGGTGATC 
ATTGGGGATC GGCAGCTGGT CAAGGGCATC ACTCGCCTAC TGGCCATTAC TGGGGAGCAG 
GCCCAACAGG CCCGAGAGGT AGGCCAGAGC CTGTCTCAGG AGGTGGAAGC AGCCAGCGAG 
CGACTAAGTC GGGGCAGCCG AGACCTGCCA GAAGCTCACC GGCTATCTAA GGACATAGGA 
CGTCTCACCG AGGTAGTAGA GTCTGCTGTG ATACCTCAGT GGCAACGGCA GGAGCTGCAG 
ACCACACTAA AGATGCTACA GCGCCGTGCC AACACTGCCA TCCGGAAACT GGAGAAGGGC 
CAGGCTACAG AGAAATCCCA GGAGCTGTTG AAACGACACT CGGAGGGGCC TCTGATTGTG 
GACACTGTCT CTGCCGAGTC CCTCTCAGTG CTGGTAAAGG TCGTGCGGCA GCTGTGCAAA 
CAGGCCCCCA GCATATCCGT GCTGCTGCTC AGCCCCCAGC CCACTGGCAG TGTCCTGTGT 
GCCTGCCAGG TGGCCCAGGG TGCCACGCCC ACCTTCACAG CTGAAGCCTG GGCACTGGCT 
GTGTGCAGCC ACATGGGAGG CAAGGCATGG GGCTCCAGAG TGGTAGCTCA GGGGACTGGA 
CACACGGCTG ACCTGGAGGC CGCCCTCGGG ACAGCCCGAG CTTACGCACT CAGCCAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aars2 ( XM_021149720.1 ) cDNA ORF clone, Mus caroli(Ryukyu mouse) -> XP_021005379.1 Mus caroli alanyl-tRNA synthetase 2, mitochondrial (Aars2), mRNA.

Price & Availability↑

CloneID	OMv35100
Clone ID Related Accession (Same CDS sequence)	XM_021149720.2 , XM_021149720.1
Accession Version	XM_021149720.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-20
Organism	Mus caroli(Ryukyu mouse)
Product	alanine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034586.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus caroli Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG CGTTGGCTGC CGCAGCCGGT AAGCTGCGGC GAGCCATTGG GAGGTCGTGC 
CCATGGCAGC CTTTCTCAAC CGAACCCGGT CCACCCCACG GAGCGGCCGT GCGGGACGCC 
TTCCTGAGCT TCTTCCGAGA TCGCCACGGC CACCGGCTCG TGCCCTCCGC TACCGTGAGG 
CCGCGCGGCG ACCCCAGCCT GCTTTTCGTC AATGCAGGCA TGAACCAGTT CAAGCCCATC 
TTCCTGGGCA CAGTGGATCC ACGAAGCGAG ATGGCAGGCT TCCGACGTGT TGCTAATAGC 
CAGAAGTGTG TCAGGGCTGG AGGACGCCAT AACGACCTGG AGGATGTGGG CCGTGATCTC 
TCTCATCATA CGTTTTTCGA GATGCTTGGC AACTGGGCTT TCGGGGGTGA ATATTTTAAG 
GAGGAAGCTT GTAGCATGGC CTGGGAACTT CTGACTCAAG TCTACGGGAT CCCTGAGGAC 
AGGTTGTGGG TCTCCTACTT CAGTGGTGAC TCCCAGACAG GACTGGACCC AGACCTGGAG 
ACCAGAGACA TCTGGCTAAG CTTGGGAGTG CCTGCCAGCC GTGTGCTCTC CTTCGGACCA 
CAAGAGAACT TCTGGGAGAT GGGAGACACT GGGCCTTGTG GGCCTTGTAC TGAGATCCAC 
TATGACCTGG CTGGGGACGT GGGAAGCCCC CAGCTGGTAG AGCTTTGGAA TCTGGTCTTC 
ATGCAACATT ACAGAGAGGC AGATGGAAGC CTGCAGCTGC TGCCGCAGCG ACATGTAGAC 
ACAGGAATGG GCCTGGAAAG GCTGGTGGCC ATCCTGCAGG GCAAACGTTC CACCTACGAC 
ACTGACCTCT TCTCTCCACT GCTCGACGCC ATACACCAGA GCTGCGGGGC TCCCCCTTAC 
TCTGGCCGGG TAGGGGCAGC AGATGAGGGC CGGATAGACA CAGCGTACCG AGTGGTAGCC 
GATCACATCC GCACACTCAG TGTCTGCATT GCTGACGGTG TCTCCCCGGG GATGTCAGGT 
GCCCCGCTCG TTCTCCGTCG GATTCTCCGC CGAGCTGTGC GCTATTCCAC AGAGGTCTTG 
CAGGCACCAC CTGGTTTTCT AGGCAGCCTG GTGCCAGTGG TGGTAGAGAC ACTGGGATCT 
GCTTATCCCG AACTTGAGAA GAACTCAGTC AAGATAGCCA GCCTGGTGTC AGAGGATGAG 
GCAGCCTTCC TGGCCTCCCT GCAGCGAGGC CGGCGGATCA TCGATCGCAC CGTCAAGCGC 
CTGGGCCCTT CTGATTTGTT CCCTGCCGAA GTGGCCTGGT CATTGTCGCT GTCTGGGAAT 
CTGGGGATTC CCCTGGACCT GGTGGAGCTG ATGCTGGAGG AGAAGGAGGT GAAGCTGGAC 
ACAGCAGGAG TGGAGCAGCT AGCCAAGAAG GAGGCTCAGC ACCGGGCCCA GCAGGCAGAG 
GCAGATCAGG AGGACCGATT GTGTCTTGAT GTCCACGCAC TGGAGGAGCT GCACCGTCAA 
GGCATACCCC CAACTGATGA CAGCCCCAAG TATAACTATT CTCTTCACCC CAACGGGGAT 
TACGAGTTTG GCCTCTGTGA GGCCCGGGTG TTACAGCTGT ATTCAGAGAC TGGGACGGCC 
GTGGCCTCCG TGGGAGCAGG CCAGCGCTGT GGCCTCCTAT TAGACAGAAC CAACTTCTAC 
GCTGAACAAG GGGGTCAGGC TTCAGACCGA GGCTACCTTG TCCGTACAGG GCAGCAGGAT 
GTGTTGTTCC CTGTGGCCGG GGCTCAGCTC TGTGGGGGTT TTATCCTGCA TGAGGCCATG 
GCTCCCGAGC ACCTACAGGT GGGGGATCAA GTGCAGCTGT ATGTAGATAC GGCCTGGCGA 
ATGGGTTGCA TGGTGAAGCA CACGGCCACC CACCTGCTGA GCTGGGCCCT TCGGCAGACC 
CTCGGACCGA CCACCGAGCA GCGGGGCTCC CATCTCAACC CCGAGCGGCT GCGCTTTGAC 
GTGGCCACCC AGACCCCACT GACCACAGAG CAGCTACGGA CGGTAGAGAG CTACGTGCAG 
GAAGTCGTGG GGCAGGATAA GCCTGTGTTC ATGGAGGAAG TGCCCCTGGC ACACACTGCC 
CGCATCCCCG GCCTTCGCTC ACTGGATGAG GTGTACCCAG ACCCCGTCCG GGTGGTGTCC 
GTTGGGGTTC CTGTTGCCCA CGCACTGGGG CCAGCCTCCC AGGCTGCAAT GCACACCTCC 
GTGGAGCTGT GCTGTGGGAC GCACCTGCTA AGTACTGGGG CCGTGGGGGA CTTGGTGATC 
ATTGGGGATC GGCAGCTGGT CAAGGGCATC ACTCGCCTAC TGGCCATTAC TGGGGAGCAG 
GCCCAACAGG CCCGAGAGGT AGGCCAGAGC CTGTCTCAGG AGGTGGAAGC AGCCAGCGAG 
CGACTAAGTC GGGGCAGCCG AGACCTGCCA GAAGCTCACC GGCTATCTAA GGACATAGGA 
CGTCTCACCG AGGTAGTAGA GTCTGCTGTG ATACCTCAGT GGCAACGGCA GGAGCTGCAG 
ACCACACTAA AGATGCTACA GCGCCGTGCC AACACTGCCA TCCGGAAACT GGAGAAGGGC 
CAGGCTACAG AGAAATCCCA GGAGCTGTTG AAACGACACT CGGAGGGGCC TCTGATTGTG 
GACACTGTCT CTGCCGAGTC CCTCTCAGTG CTGGTAAAGG TCGTGCGGCA GCTGTGCAAA 
CAGGCCCCCA GCATATCCGT GCTGCTGCTC AGCCCCCAGC CCACTGGCAG TGTCCTGTGT 
GCCTGCCAGG TGGCCCAGGG TGCCACGCCC ACCTTCACAG CTGAAGCCTG GGCACTGGCT 
GTGTGCAGCC ACATGGGAGG CAAGGCATGG GGCTCCAGAG TGGTAGCTCA GGGGACTGGA 
CACACGGCTG ACCTGGAGGC CGCCCTCGGG ACAGCCCGAG CTTACGCACT CAGCCAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021005379.1
CDS	64..3006
Translation	MAVALAAAAGKLRRAIGRSCPWQPFSTEPGPPHGAAVRDAFLSFFRDRHGHRLVPSATVRPRGDPSLLFVNAGMNQFKPIFLGTVDPRSEMAGFRRVANSQKCVRAGGRHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFKEEACSMAWELLTQVYGIPEDRLWVSYFSGDSQTGLDPDLETRDIWLSLGVPASRVLSFGPQENFWEMGDTGPCGPCTEIHYDLAGDVGSPQLVELWNLVFMQHYREADGSLQLLPQRHVDTGMGLERLVAILQGKRSTYDTDLFSPLLDAIHQSCGAPPYSGRVGAADEGRIDTAYRVVADHIRTLSVCIADGVSPGMSGAPLVLRRILRRAVRYSTEVLQAPPGFLGSLVPVVVETLGSAYPELEKNSVKIASLVSEDEAAFLASLQRGRRIIDRTVKRLGPSDLFPAEVAWSLSLSGNLGIPLDLVELMLEEKEVKLDTAGVEQLAKKEAQHRAQQAEADQEDRLCLDVHALEELHRQGIPPTDDSPKYNYSLHPNGDYEFGLCEARVLQLYSETGTAVASVGAGQRCGLLLDRTNFYAEQGGQASDRGYLVRTGQQDVLFPVAGAQLCGGFILHEAMAPEHLQVGDQVQLYVDTAWRMGCMVKHTATHLLSWALRQTLGPTTEQRGSHLNPERLRFDVATQTPLTTEQLRTVESYVQEVVGQDKPVFMEEVPLAHTARIPGLRSLDEVYPDPVRVVSVGVPVAHALGPASQAAMHTSVELCCGTHLLSTGAVGDLVIIGDRQLVKGITRLLAITGEQAQQAREVGQSLSQEVEAASERLSRGSRDLPEAHRLSKDIGRLTEVVESAVIPQWQRQELQTTLKMLQRRANTAIRKLEKGQATEKSQELLKRHSEGPLIVDTVSAESLSVLVKVVRQLCKQAPSISVLLLSPQPTGSVLCACQVAQGATPTFTAEAWALAVCSHMGGKAWGSRVVAQGTGHTADLEAALGTARAYALSQL

Target ORF information:

RefSeq Version	XM_021149720.1
Organism	Mus caroli(Ryukyu mouse)
Definition	Mus caroli alanyl-tRNA synthetase 2, mitochondrial (Aars2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021149720.1

ORF Insert Sequence:

ATGGCGGTGG CGTTGGCTGC CGCAGCCGGT AAGCTGCGGC GAGCCATTGG GAGGTCGTGC 
CCATGGCAGC CTTTCTCAAC CGAACCCGGT CCACCCCACG GAGCGGCCGT GCGGGACGCC 
TTCCTGAGCT TCTTCCGAGA TCGCCACGGC CACCGGCTCG TGCCCTCCGC TACCGTGAGG 
CCGCGCGGCG ACCCCAGCCT GCTTTTCGTC AATGCAGGCA TGAACCAGTT CAAGCCCATC 
TTCCTGGGCA CAGTGGATCC ACGAAGCGAG ATGGCAGGCT TCCGACGTGT TGCTAATAGC 
CAGAAGTGTG TCAGGGCTGG AGGACGCCAT AACGACCTGG AGGATGTGGG CCGTGATCTC 
TCTCATCATA CGTTTTTCGA GATGCTTGGC AACTGGGCTT TCGGGGGTGA ATATTTTAAG 
GAGGAAGCTT GTAGCATGGC CTGGGAACTT CTGACTCAAG TCTACGGGAT CCCTGAGGAC 
AGGTTGTGGG TCTCCTACTT CAGTGGTGAC TCCCAGACAG GACTGGACCC AGACCTGGAG 
ACCAGAGACA TCTGGCTAAG CTTGGGAGTG CCTGCCAGCC GTGTGCTCTC CTTCGGACCA 
CAAGAGAACT TCTGGGAGAT GGGAGACACT GGGCCTTGTG GGCCTTGTAC TGAGATCCAC 
TATGACCTGG CTGGGGACGT GGGAAGCCCC CAGCTGGTAG AGCTTTGGAA TCTGGTCTTC 
ATGCAACATT ACAGAGAGGC AGATGGAAGC CTGCAGCTGC TGCCGCAGCG ACATGTAGAC 
ACAGGAATGG GCCTGGAAAG GCTGGTGGCC ATCCTGCAGG GCAAACGTTC CACCTACGAC 
ACTGACCTCT TCTCTCCACT GCTCGACGCC ATACACCAGA GCTGCGGGGC TCCCCCTTAC 
TCTGGCCGGG TAGGGGCAGC AGATGAGGGC CGGATAGACA CAGCGTACCG AGTGGTAGCC 
GATCACATCC GCACACTCAG TGTCTGCATT GCTGACGGTG TCTCCCCGGG GATGTCAGGT 
GCCCCGCTCG TTCTCCGTCG GATTCTCCGC CGAGCTGTGC GCTATTCCAC AGAGGTCTTG 
CAGGCACCAC CTGGTTTTCT AGGCAGCCTG GTGCCAGTGG TGGTAGAGAC ACTGGGATCT 
GCTTATCCCG AACTTGAGAA GAACTCAGTC AAGATAGCCA GCCTGGTGTC AGAGGATGAG 
GCAGCCTTCC TGGCCTCCCT GCAGCGAGGC CGGCGGATCA TCGATCGCAC CGTCAAGCGC 
CTGGGCCCTT CTGATTTGTT CCCTGCCGAA GTGGCCTGGT CATTGTCGCT GTCTGGGAAT 
CTGGGGATTC CCCTGGACCT GGTGGAGCTG ATGCTGGAGG AGAAGGAGGT GAAGCTGGAC 
ACAGCAGGAG TGGAGCAGCT AGCCAAGAAG GAGGCTCAGC ACCGGGCCCA GCAGGCAGAG 
GCAGATCAGG AGGACCGATT GTGTCTTGAT GTCCACGCAC TGGAGGAGCT GCACCGTCAA 
GGCATACCCC CAACTGATGA CAGCCCCAAG TATAACTATT CTCTTCACCC CAACGGGGAT 
TACGAGTTTG GCCTCTGTGA GGCCCGGGTG TTACAGCTGT ATTCAGAGAC TGGGACGGCC 
GTGGCCTCCG TGGGAGCAGG CCAGCGCTGT GGCCTCCTAT TAGACAGAAC CAACTTCTAC 
GCTGAACAAG GGGGTCAGGC TTCAGACCGA GGCTACCTTG TCCGTACAGG GCAGCAGGAT 
GTGTTGTTCC CTGTGGCCGG GGCTCAGCTC TGTGGGGGTT TTATCCTGCA TGAGGCCATG 
GCTCCCGAGC ACCTACAGGT GGGGGATCAA GTGCAGCTGT ATGTAGATAC GGCCTGGCGA 
ATGGGTTGCA TGGTGAAGCA CACGGCCACC CACCTGCTGA GCTGGGCCCT TCGGCAGACC 
CTCGGACCGA CCACCGAGCA GCGGGGCTCC CATCTCAACC CCGAGCGGCT GCGCTTTGAC 
GTGGCCACCC AGACCCCACT GACCACAGAG CAGCTACGGA CGGTAGAGAG CTACGTGCAG 
GAAGTCGTGG GGCAGGATAA GCCTGTGTTC ATGGAGGAAG TGCCCCTGGC ACACACTGCC 
CGCATCCCCG GCCTTCGCTC ACTGGATGAG GTGTACCCAG ACCCCGTCCG GGTGGTGTCC 
GTTGGGGTTC CTGTTGCCCA CGCACTGGGG CCAGCCTCCC AGGCTGCAAT GCACACCTCC 
GTGGAGCTGT GCTGTGGGAC GCACCTGCTA AGTACTGGGG CCGTGGGGGA CTTGGTGATC 
ATTGGGGATC GGCAGCTGGT CAAGGGCATC ACTCGCCTAC TGGCCATTAC TGGGGAGCAG 
GCCCAACAGG CCCGAGAGGT AGGCCAGAGC CTGTCTCAGG AGGTGGAAGC AGCCAGCGAG 
CGACTAAGTC GGGGCAGCCG AGACCTGCCA GAAGCTCACC GGCTATCTAA GGACATAGGA 
CGTCTCACCG AGGTAGTAGA GTCTGCTGTG ATACCTCAGT GGCAACGGCA GGAGCTGCAG 
ACCACACTAA AGATGCTACA GCGCCGTGCC AACACTGCCA TCCGGAAACT GGAGAAGGGC 
CAGGCTACAG AGAAATCCCA GGAGCTGTTG AAACGACACT CGGAGGGGCC TCTGATTGTG 
GACACTGTCT CTGCCGAGTC CCTCTCAGTG CTGGTAAAGG TCGTGCGGCA GCTGTGCAAA 
CAGGCCCCCA GCATATCCGT GCTGCTGCTC AGCCCCCAGC CCACTGGCAG TGTCCTGTGT 
GCCTGCCAGG TGGCCCAGGG TGCCACGCCC ACCTTCACAG CTGAAGCCTG GGCACTGGCT 
GTGTGCAGCC ACATGGGAGG CAAGGCATGG GGCTCCAGAG TGGTAGCTCA GGGGACTGGA 
CACACGGCTG ACCTGGAGGC CGCCCTCGGG ACAGCCCGAG CTTACGCACT CAGCCAGCTC 
TGA