LOC11427149 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC11427149
Entrez Gene ID	11427149
Full Name	beta-like galactosidase
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013610029.1	XP_013465483.1	beta-like galactosidase
XM_024776825.1	XP_024632593.1	beta-galactosidase 17 isoform X2
XM_003597550.2	XP_003597598.1	beta-like galactosidase
XM_003597550.3	XP_003597598.1	beta-galactosidase 17 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC11427149 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC11427149 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe206067	XM_013610029.1 Latest version!	Medicago truncatula beta-like galactosidase mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OMe207330	XM_003597550.3 Latest version!	Medicago truncatula beta-galactosidase 17 (LOC11427149), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe260065	XM_024776825.1 Latest version!	Medicago truncatula beta-galactosidase 17 (LOC11427149), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMe207330	XM_003597550.2	Medicago truncatula beta-like galactosidase mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC11427149 ( XM_013610029.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013465483.1 Medicago truncatula beta-like galactosidase mRNA.

Price & Availability↑

CloneID	OMe206067
Clone ID Related Accession (Same CDS sequence)	XM_013610029.1
Accession Version	XM_013610029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	beta-like galactosidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016408). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGTTCGAC CCGGACCATA TATATGTGCA GAGTGGGACT GGGGTGGTAT CCCAAGTTGG 
TTATTTTCCA GGAATCCAAC CCCAAAACCA AGATCATCTG ATCCTGCTTT TCTTAAACTG 
GTTGAAAGAT GGTGGGGCAA ACTGCTTCCA AAATTAGTTC CTCTCCTATA TGACAACGGA 
GGCCCTATAA TAATGGTTCA GATTGAGAAT GAGTATGGTT CTTATGGAGA CGACAAAGCA 
TATCTCCATC ACCTGATCAC ACTAGCTAGA GGTCATCTTG GGCAGGATGC CATTTTATAC 
ACTACAGATG GAGGGTCCAG GGAAAATCTT GAGAAAGGAA CTATTCGTGG AGATACTGTC 
TTTTCAGCTG TTGACTTCAC TACTGGTGAT GATCCTTGGC CTATATTCAA GTTACAGAAG 
GAGTTCAATG CCCCAGGAAA ATCGCCACCT TTGTCATCCG AATTTTACAC TGGGTGGCTT 
ACACATTGGG GAGAGAAAAA TGCTAAGACC GATGCTGATT CCACAGCAGC TGCTCTTGAA 
GAAATTTTGA GAAAAAATGG GTCTGCCGTC CTATACATGG CGCATGGTGG TTCAAACTTT 
GGATTTTATA ATGGAGCAAA TACCGGTGCA AACGAGGCTG ACTATAAACC TGATCTCACT 
TCATATGATT ATGATGCCCC GATCAGGGAA GCTGGTGATG TTGACAATTC AAAATTCAAT 
GCAATTCGGA GGGTTATATC CCGATATAGC TCTGCACCTC TTCCTTCTAT TCCCTCTTAT 
AATGAAAAGA CAACATATGG ACCAATCCAC TTGCAAAGAC GATCTTCTTT ATTTGATATA 
TTTGATTTCA CCAATTCCTC CAGCTCCTTT GAATCAGAAA ATCCAATGTC AATGGAGAAT 
GTGGGCCAGT TTTTTGGTTT CTTATTGTAT GTCACTGATT ACGAAGCAAG GAGAGGGGGA 
AGAAATTTGT CCATACCAAA GGTGCATGAT AGAGCTCAAG TATTTATCTC ATGTTCTTCT 
AAAGGAAGGG GTACAAGGCC AACTTATGTT GGTACTGTCG AAAGATGGTT GAATAAAAAA 
CTCAGCCTCC CTGAATATCA ATGCCATTCT AAGATCAACT TATATATCTT GGTTGAAAAC 
ATGGGACGTG TAAATTATGG GCCTTTCATC TTCGATAGGA AGGGTATTCT GTCTTCTGTT 
TATCTGGATG GGAATAGAGT ACAAGGTTGG AAAATGTTCC CTATTCCTTT GCACAATCTA 
AACGAAGTGC CAAATTACAA TCGCATAATG CAAGCTTCAT ATTCTGCATT CGGTGAAATA 
TCAACCTCCA GGAAAAGGTT GATGAATAAG TCTGAGAATA CTTCAAAAGA ACCTGCTTTT 
TATTCTGGAC ACTTCTTAAT TGATAAAACA AGTCAGGTCA AGGATACATT TTTGTCATTC 
AGAAATTGGG GGAAAGGCGT TGTGTTTGTT AATGACTTCA ATCTAGGAAG ATATTGGCCG 
TTGAGAGGAC CACAGTGCAA CCTCTACGTT CCTGCTCCAG TCCTTAAGCA AGGAGATAAT 
TTTGTGGTAA TATTCGAGTT GGAATCTCCA GACCCCAATC TTTTGGTGCA CTCGGTTGAT 
GAGCCAGACT ACACATGTGG CTTTAATGGG ATGAACATAC ATCAGTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013465483.1
CDS	294..1964
Translation	MVRPGPYICAEWDWGGIPSWLFSRNPTPKPRSSDPAFLKLVERWWGKLLPKLVPLLYDNGGPIIMVQIENEYGSYGDDKAYLHHLITLARGHLGQDAILYTTDGGSRENLEKGTIRGDTVFSAVDFTTGDDPWPIFKLQKEFNAPGKSPPLSSEFYTGWLTHWGEKNAKTDADSTAAALEEILRKNGSAVLYMAHGGSNFGFYNGANTGANEADYKPDLTSYDYDAPIREAGDVDNSKFNAIRRVISRYSSAPLPSIPSYNEKTTYGPIHLQRRSSLFDIFDFTNSSSSFESENPMSMENVGQFFGFLLYVTDYEARRGGRNLSIPKVHDRAQVFISCSSKGRGTRPTYVGTVERWLNKKLSLPEYQCHSKINLYILVENMGRVNYGPFIFDRKGILSSVYLDGNRVQGWKMFPIPLHNLNEVPNYNRIMQASYSAFGEISTSRKRLMNKSENTSKEPAFYSGHFLIDKTSQVKDTFLSFRNWGKGVVFVNDFNLGRYWPLRGPQCNLYVPAPVLKQGDNFVVIFELESPDPNLLVHSVDEPDYTCGFNGMNIHQL

Target ORF information:

RefSeq Version	XM_013610029.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula beta-like galactosidase mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013610029.1

ORF Insert Sequence:

ATGGTTCGAC CCGGACCATA TATATGTGCA GAGTGGGACT GGGGTGGTAT CCCAAGTTGG 
TTATTTTCCA GGAATCCAAC CCCAAAACCA AGATCATCTG ATCCTGCTTT TCTTAAACTG 
GTTGAAAGAT GGTGGGGCAA ACTGCTTCCA AAATTAGTTC CTCTCCTATA TGACAACGGA 
GGCCCTATAA TAATGGTTCA GATTGAGAAT GAGTATGGTT CTTATGGAGA CGACAAAGCA 
TATCTCCATC ACCTGATCAC ACTAGCTAGA GGTCATCTTG GGCAGGATGC CATTTTATAC 
ACTACAGATG GAGGGTCCAG GGAAAATCTT GAGAAAGGAA CTATTCGTGG AGATACTGTC 
TTTTCAGCTG TTGACTTCAC TACTGGTGAT GATCCTTGGC CTATATTCAA GTTACAGAAG 
GAGTTCAATG CCCCAGGAAA ATCGCCACCT TTGTCATCCG AATTTTACAC TGGGTGGCTT 
ACACATTGGG GAGAGAAAAA TGCTAAGACC GATGCTGATT CCACAGCAGC TGCTCTTGAA 
GAAATTTTGA GAAAAAATGG GTCTGCCGTC CTATACATGG CGCATGGTGG TTCAAACTTT 
GGATTTTATA ATGGAGCAAA TACCGGTGCA AACGAGGCTG ACTATAAACC TGATCTCACT 
TCATATGATT ATGATGCCCC GATCAGGGAA GCTGGTGATG TTGACAATTC AAAATTCAAT 
GCAATTCGGA GGGTTATATC CCGATATAGC TCTGCACCTC TTCCTTCTAT TCCCTCTTAT 
AATGAAAAGA CAACATATGG ACCAATCCAC TTGCAAAGAC GATCTTCTTT ATTTGATATA 
TTTGATTTCA CCAATTCCTC CAGCTCCTTT GAATCAGAAA ATCCAATGTC AATGGAGAAT 
GTGGGCCAGT TTTTTGGTTT CTTATTGTAT GTCACTGATT ACGAAGCAAG GAGAGGGGGA 
AGAAATTTGT CCATACCAAA GGTGCATGAT AGAGCTCAAG TATTTATCTC ATGTTCTTCT 
AAAGGAAGGG GTACAAGGCC AACTTATGTT GGTACTGTCG AAAGATGGTT GAATAAAAAA 
CTCAGCCTCC CTGAATATCA ATGCCATTCT AAGATCAACT TATATATCTT GGTTGAAAAC 
ATGGGACGTG TAAATTATGG GCCTTTCATC TTCGATAGGA AGGGTATTCT GTCTTCTGTT 
TATCTGGATG GGAATAGAGT ACAAGGTTGG AAAATGTTCC CTATTCCTTT GCACAATCTA 
AACGAAGTGC CAAATTACAA TCGCATAATG CAAGCTTCAT ATTCTGCATT CGGTGAAATA 
TCAACCTCCA GGAAAAGGTT GATGAATAAG TCTGAGAATA CTTCAAAAGA ACCTGCTTTT 
TATTCTGGAC ACTTCTTAAT TGATAAAACA AGTCAGGTCA AGGATACATT TTTGTCATTC 
AGAAATTGGG GGAAAGGCGT TGTGTTTGTT AATGACTTCA ATCTAGGAAG ATATTGGCCG 
TTGAGAGGAC CACAGTGCAA CCTCTACGTT CCTGCTCCAG TCCTTAAGCA AGGAGATAAT 
TTTGTGGTAA TATTCGAGTT GGAATCTCCA GACCCCAATC TTTTGGTGCA CTCGGTTGAT 
GAGCCAGACT ACACATGTGG CTTTAATGGG ATGAACATAC ATCAGTTGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11427149 ( XM_003597550.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003597598.1 Medicago truncatula beta-like galactosidase mRNA.

Price & Availability↑

CloneID	OMe207330
Clone ID Related Accession (Same CDS sequence)	XM_003597550.2 , XM_003597550.3
Accession Version	XM_003597550.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	beta-like galactosidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016408). On Aug 25, 2015 this sequence version replaced XM_003597550.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGGCGAAGA AACGAATTTC CACAGCTACC CTTTTCTTCA TCTTCATCTC TTTCATTGCT 
CTTTGTGCCA TTGTTCCTGT TTTTGCTCCT CTTCCTTCAA TATCATCTCG CCAGCATCAT 
CATGCCCATC ATTCTCATTC CAAAAAAGTT AATAAGAAGT TCGAGATTGC AAATGATATG 
TTTTGGAAAG ATGGTGAACC TTTTCGGATC ATCGGTGGTG ACTTGCATTA TTTTCGAGTG 
CATCCTGAGT ATTGGGAAGA TAGATTGTTA AAAGCCAAGG CATTGGGCTT GAACACAATT 
CAGACATATG TTCCTTGGAA TCTGCATGAA CCAGCACCAG AAAAGCTCGT TTTTGAGGGT 
ATTGCAAATA TAGAATCATT CCTCAACCTT TGTCACAAAC TCGATCTCCT CGTGATGGTT 
CGACCCGGAC CATATATATG TGCAGAGTGG GACTGGGGTG GTATCCCAAG TTGGTTATTT 
TCCAGGAATC CAACCCCAAA ACCAAGATCA TCTGATCCTG CTTTTCTTAA ACTGGTTGAA 
AGATGGTGGG GCAAACTGCT TCCAAAATTA GTTCCTCTCC TATATGACAA CGGAGGCCCT 
ATAATAATGG TTCAGATTGA GAATGAGTAT GGTTCTTATG GAGACGACAA AGCATATCTC 
CATCACCTGA TCACACTAGC TAGAGGTCAT CTTGGGCAGG ATGCCATTTT ATACACTACA 
GATGGAGGGT CCAGGGAAAA TCTTGAGAAA GGAACTATTC GTGGAGATAC TGTCTTTTCA 
GCTGTTGACT TCACTACTGG TGATGATCCT TGGCCTATAT TCAAGTTACA GAAGGAGTTC 
AATGCCCCAG GAAAATCGCC ACCTTTGTCA TCCGAATTTT ACACTGGGTG GCTTACACAT 
TGGGGAGAGA AAAATGCTAA GACCGATGCT GATTCCACAG CAGCTGCTCT TGAAGAAATT 
TTGAGAAAAA ATGGGTCTGC CGTCCTATAC ATGGCGCATG GTGGTTCAAA CTTTGGATTT 
TATAATGGAG CAAATACCGG TGCAAACGAG GCTGACTATA AACCTGATCT CACTTCATAT 
GATTATGATG CCCCGATCAG GGAAGCTGGT GATGTTGACA ATTCAAAATT CAATGCAATT 
CGGAGGGTTA TATCCCGATA TAGCTCTGCA CCTCTTCCTT CTATTCCCTC TTATAATGAA 
AAGACAACAT ATGGACCAAT CCACTTGCAA AGACGATCTT CTTTATTTGA TATATTTGAT 
TTCACCAATT CCTCCAGCTC CTTTGAATCA GAAAATCCAA TGTCAATGGA GAATGTGGGC 
CAGTTTTTTG GTTTCTTATT GTATGTCACT GATTACGAAG CAAGGAGAGG GGGAAGAAAT 
TTGTCCATAC CAAAGGTGCA TGATAGAGCT CAAGTATTTA TCTCATGTTC TTCTAAAGGA 
AGGGGTACAA GGCCAACTTA TGTTGGTACT GTCGAAAGAT GGTTGAATAA AAAACTCAGC 
CTCCCTGAAT ATCAATGCCA TTCTAAGATC AACTTATATA TCTTGGTTGA AAACATGGGA 
CGTGTAAATT ATGGGCCTTT CATCTTCGAT AGGAAGGGTA TTCTGTCTTC TGTTTATCTG 
GATGGGAATA GAGTACAAGG TTGGAAAATG TTCCCTATTC CTTTGCACAA TCTAAACGAA 
GTGCCAAATT ACAATCGCAT AATGCAAGCT TCATATTCTG CATTCGGTGA AATATCAACC 
TCCAGGAAAA GGTTGATGAA TAAGTCTGAG AATACTTCAA AAGAACCTGC TTTTTATTCT 
GGACACTTCT TAATTGATAA AACAAGTCAG GTCAAGGATA CATTTTTGTC ATTCAGAAAT 
TGGGGGAAAG GCGTTGTGTT TGTTAATGAC TTCAATCTAG GAAGATATTG GCCGTTGAGA 
GGACCACAGT GCAACCTCTA CGTTCCTGCT CCAGTCCTTA AGCAAGGAGA TAATTTTGTG 
GTAATATTCG AGTTGGAATC TCCAGACCCC AATCTTTTGG TGCACTCGGT TGATGAGCCA 
GACTACACAT GTGGCTTTAA TGGGATGAAC ATACATCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003597598.1
CDS	84..2168
Translation	MAKKRISTATLFFIFISFIALCAIVPVFAPLPSISSRQHHHAHHSHSKKVNKKFEIANDMFWKDGEPFRIIGGDLHYFRVHPEYWEDRLLKAKALGLNTIQTYVPWNLHEPAPEKLVFEGIANIESFLNLCHKLDLLVMVRPGPYICAEWDWGGIPSWLFSRNPTPKPRSSDPAFLKLVERWWGKLLPKLVPLLYDNGGPIIMVQIENEYGSYGDDKAYLHHLITLARGHLGQDAILYTTDGGSRENLEKGTIRGDTVFSAVDFTTGDDPWPIFKLQKEFNAPGKSPPLSSEFYTGWLTHWGEKNAKTDADSTAAALEEILRKNGSAVLYMAHGGSNFGFYNGANTGANEADYKPDLTSYDYDAPIREAGDVDNSKFNAIRRVISRYSSAPLPSIPSYNEKTTYGPIHLQRRSSLFDIFDFTNSSSSFESENPMSMENVGQFFGFLLYVTDYEARRGGRNLSIPKVHDRAQVFISCSSKGRGTRPTYVGTVERWLNKKLSLPEYQCHSKINLYILVENMGRVNYGPFIFDRKGILSSVYLDGNRVQGWKMFPIPLHNLNEVPNYNRIMQASYSAFGEISTSRKRLMNKSENTSKEPAFYSGHFLIDKTSQVKDTFLSFRNWGKGVVFVNDFNLGRYWPLRGPQCNLYVPAPVLKQGDNFVVIFELESPDPNLLVHSVDEPDYTCGFNGMNIHQL

Target ORF information:

RefSeq Version	XM_003597550.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula beta-like galactosidase mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003597550.2

ORF Insert Sequence:

ATGGCGAAGA AACGAATTTC CACAGCTACC CTTTTCTTCA TCTTCATCTC TTTCATTGCT 
CTTTGTGCCA TTGTTCCTGT TTTTGCTCCT CTTCCTTCAA TATCATCTCG CCAGCATCAT 
CATGCCCATC ATTCTCATTC CAAAAAAGTT AATAAGAAGT TCGAGATTGC AAATGATATG 
TTTTGGAAAG ATGGTGAACC TTTTCGGATC ATCGGTGGTG ACTTGCATTA TTTTCGAGTG 
CATCCTGAGT ATTGGGAAGA TAGATTGTTA AAAGCCAAGG CATTGGGCTT GAACACAATT 
CAGACATATG TTCCTTGGAA TCTGCATGAA CCAGCACCAG AAAAGCTCGT TTTTGAGGGT 
ATTGCAAATA TAGAATCATT CCTCAACCTT TGTCACAAAC TCGATCTCCT CGTGATGGTT 
CGACCCGGAC CATATATATG TGCAGAGTGG GACTGGGGTG GTATCCCAAG TTGGTTATTT 
TCCAGGAATC CAACCCCAAA ACCAAGATCA TCTGATCCTG CTTTTCTTAA ACTGGTTGAA 
AGATGGTGGG GCAAACTGCT TCCAAAATTA GTTCCTCTCC TATATGACAA CGGAGGCCCT 
ATAATAATGG TTCAGATTGA GAATGAGTAT GGTTCTTATG GAGACGACAA AGCATATCTC 
CATCACCTGA TCACACTAGC TAGAGGTCAT CTTGGGCAGG ATGCCATTTT ATACACTACA 
GATGGAGGGT CCAGGGAAAA TCTTGAGAAA GGAACTATTC GTGGAGATAC TGTCTTTTCA 
GCTGTTGACT TCACTACTGG TGATGATCCT TGGCCTATAT TCAAGTTACA GAAGGAGTTC 
AATGCCCCAG GAAAATCGCC ACCTTTGTCA TCCGAATTTT ACACTGGGTG GCTTACACAT 
TGGGGAGAGA AAAATGCTAA GACCGATGCT GATTCCACAG CAGCTGCTCT TGAAGAAATT 
TTGAGAAAAA ATGGGTCTGC CGTCCTATAC ATGGCGCATG GTGGTTCAAA CTTTGGATTT 
TATAATGGAG CAAATACCGG TGCAAACGAG GCTGACTATA AACCTGATCT CACTTCATAT 
GATTATGATG CCCCGATCAG GGAAGCTGGT GATGTTGACA ATTCAAAATT CAATGCAATT 
CGGAGGGTTA TATCCCGATA TAGCTCTGCA CCTCTTCCTT CTATTCCCTC TTATAATGAA 
AAGACAACAT ATGGACCAAT CCACTTGCAA AGACGATCTT CTTTATTTGA TATATTTGAT 
TTCACCAATT CCTCCAGCTC CTTTGAATCA GAAAATCCAA TGTCAATGGA GAATGTGGGC 
CAGTTTTTTG GTTTCTTATT GTATGTCACT GATTACGAAG CAAGGAGAGG GGGAAGAAAT 
TTGTCCATAC CAAAGGTGCA TGATAGAGCT CAAGTATTTA TCTCATGTTC TTCTAAAGGA 
AGGGGTACAA GGCCAACTTA TGTTGGTACT GTCGAAAGAT GGTTGAATAA AAAACTCAGC 
CTCCCTGAAT ATCAATGCCA TTCTAAGATC AACTTATATA TCTTGGTTGA AAACATGGGA 
CGTGTAAATT ATGGGCCTTT CATCTTCGAT AGGAAGGGTA TTCTGTCTTC TGTTTATCTG 
GATGGGAATA GAGTACAAGG TTGGAAAATG TTCCCTATTC CTTTGCACAA TCTAAACGAA 
GTGCCAAATT ACAATCGCAT AATGCAAGCT TCATATTCTG CATTCGGTGA AATATCAACC 
TCCAGGAAAA GGTTGATGAA TAAGTCTGAG AATACTTCAA AAGAACCTGC TTTTTATTCT 
GGACACTTCT TAATTGATAA AACAAGTCAG GTCAAGGATA CATTTTTGTC ATTCAGAAAT 
TGGGGGAAAG GCGTTGTGTT TGTTAATGAC TTCAATCTAG GAAGATATTG GCCGTTGAGA 
GGACCACAGT GCAACCTCTA CGTTCCTGCT CCAGTCCTTA AGCAAGGAGA TAATTTTGTG 
GTAATATTCG AGTTGGAATC TCCAGACCCC AATCTTTTGG TGCACTCGGT TGATGAGCCA 
GACTACACAT GTGGCTTTAA TGGGATGAAC ATACATCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11427149 ( XM_003597550.3 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003597598.1 Medicago truncatula beta-galactosidase 17 (LOC11427149), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMe207330
Clone ID Related Accession (Same CDS sequence)	XM_003597550.2 , XM_003597550.3
Accession Version	XM_003597550.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	beta-galactosidase 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016408.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_003597550.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGA AACGAATTTC CACAGCTACC CTTTTCTTCA TCTTCATCTC TTTCATTGCT 
CTTTGTGCCA TTGTTCCTGT TTTTGCTCCT CTTCCTTCAA TATCATCTCG CCAGCATCAT 
CATGCCCATC ATTCTCATTC CAAAAAAGTT AATAAGAAGT TCGAGATTGC AAATGATATG 
TTTTGGAAAG ATGGTGAACC TTTTCGGATC ATCGGTGGTG ACTTGCATTA TTTTCGAGTG 
CATCCTGAGT ATTGGGAAGA TAGATTGTTA AAAGCCAAGG CATTGGGCTT GAACACAATT 
CAGACATATG TTCCTTGGAA TCTGCATGAA CCAGCACCAG AAAAGCTCGT TTTTGAGGGT 
ATTGCAAATA TAGAATCATT CCTCAACCTT TGTCACAAAC TCGATCTCCT CGTGATGGTT 
CGACCCGGAC CATATATATG TGCAGAGTGG GACTGGGGTG GTATCCCAAG TTGGTTATTT 
TCCAGGAATC CAACCCCAAA ACCAAGATCA TCTGATCCTG CTTTTCTTAA ACTGGTTGAA 
AGATGGTGGG GCAAACTGCT TCCAAAATTA GTTCCTCTCC TATATGACAA CGGAGGCCCT 
ATAATAATGG TTCAGATTGA GAATGAGTAT GGTTCTTATG GAGACGACAA AGCATATCTC 
CATCACCTGA TCACACTAGC TAGAGGTCAT CTTGGGCAGG ATGCCATTTT ATACACTACA 
GATGGAGGGT CCAGGGAAAA TCTTGAGAAA GGAACTATTC GTGGAGATAC TGTCTTTTCA 
GCTGTTGACT TCACTACTGG TGATGATCCT TGGCCTATAT TCAAGTTACA GAAGGAGTTC 
AATGCCCCAG GAAAATCGCC ACCTTTGTCA TCCGAATTTT ACACTGGGTG GCTTACACAT 
TGGGGAGAGA AAAATGCTAA GACCGATGCT GATTCCACAG CAGCTGCTCT TGAAGAAATT 
TTGAGAAAAA ATGGGTCTGC CGTCCTATAC ATGGCGCATG GTGGTTCAAA CTTTGGATTT 
TATAATGGAG CAAATACCGG TGCAAACGAG GCTGACTATA AACCTGATCT CACTTCATAT 
GATTATGATG CCCCGATCAG GGAAGCTGGT GATGTTGACA ATTCAAAATT CAATGCAATT 
CGGAGGGTTA TATCCCGATA TAGCTCTGCA CCTCTTCCTT CTATTCCCTC TTATAATGAA 
AAGACAACAT ATGGACCAAT CCACTTGCAA AGACGATCTT CTTTATTTGA TATATTTGAT 
TTCACCAATT CCTCCAGCTC CTTTGAATCA GAAAATCCAA TGTCAATGGA GAATGTGGGC 
CAGTTTTTTG GTTTCTTATT GTATGTCACT GATTACGAAG CAAGGAGAGG GGGAAGAAAT 
TTGTCCATAC CAAAGGTGCA TGATAGAGCT CAAGTATTTA TCTCATGTTC TTCTAAAGGA 
AGGGGTACAA GGCCAACTTA TGTTGGTACT GTCGAAAGAT GGTTGAATAA AAAACTCAGC 
CTCCCTGAAT ATCAATGCCA TTCTAAGATC AACTTATATA TCTTGGTTGA AAACATGGGA 
CGTGTAAATT ATGGGCCTTT CATCTTCGAT AGGAAGGGTA TTCTGTCTTC TGTTTATCTG 
GATGGGAATA GAGTACAAGG TTGGAAAATG TTCCCTATTC CTTTGCACAA TCTAAACGAA 
GTGCCAAATT ACAATCGCAT AATGCAAGCT TCATATTCTG CATTCGGTGA AATATCAACC 
TCCAGGAAAA GGTTGATGAA TAAGTCTGAG AATACTTCAA AAGAACCTGC TTTTTATTCT 
GGACACTTCT TAATTGATAA AACAAGTCAG GTCAAGGATA CATTTTTGTC ATTCAGAAAT 
TGGGGGAAAG GCGTTGTGTT TGTTAATGAC TTCAATCTAG GAAGATATTG GCCGTTGAGA 
GGACCACAGT GCAACCTCTA CGTTCCTGCT CCAGTCCTTA AGCAAGGAGA TAATTTTGTG 
GTAATATTCG AGTTGGAATC TCCAGACCCC AATCTTTTGG TGCACTCGGT TGATGAGCCA 
GACTACACAT GTGGCTTTAA TGGGATGAAC ATACATCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003597598.1
CDS	89..2173
Translation	MAKKRISTATLFFIFISFIALCAIVPVFAPLPSISSRQHHHAHHSHSKKVNKKFEIANDMFWKDGEPFRIIGGDLHYFRVHPEYWEDRLLKAKALGLNTIQTYVPWNLHEPAPEKLVFEGIANIESFLNLCHKLDLLVMVRPGPYICAEWDWGGIPSWLFSRNPTPKPRSSDPAFLKLVERWWGKLLPKLVPLLYDNGGPIIMVQIENEYGSYGDDKAYLHHLITLARGHLGQDAILYTTDGGSRENLEKGTIRGDTVFSAVDFTTGDDPWPIFKLQKEFNAPGKSPPLSSEFYTGWLTHWGEKNAKTDADSTAAALEEILRKNGSAVLYMAHGGSNFGFYNGANTGANEADYKPDLTSYDYDAPIREAGDVDNSKFNAIRRVISRYSSAPLPSIPSYNEKTTYGPIHLQRRSSLFDIFDFTNSSSSFESENPMSMENVGQFFGFLLYVTDYEARRGGRNLSIPKVHDRAQVFISCSSKGRGTRPTYVGTVERWLNKKLSLPEYQCHSKINLYILVENMGRVNYGPFIFDRKGILSSVYLDGNRVQGWKMFPIPLHNLNEVPNYNRIMQASYSAFGEISTSRKRLMNKSENTSKEPAFYSGHFLIDKTSQVKDTFLSFRNWGKGVVFVNDFNLGRYWPLRGPQCNLYVPAPVLKQGDNFVVIFELESPDPNLLVHSVDEPDYTCGFNGMNIHQL

Target ORF information:

RefSeq Version	XM_003597550.3
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula beta-galactosidase 17 (LOC11427149), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003597550.3

ORF Insert Sequence:

ATGGCGAAGA AACGAATTTC CACAGCTACC CTTTTCTTCA TCTTCATCTC TTTCATTGCT 
CTTTGTGCCA TTGTTCCTGT TTTTGCTCCT CTTCCTTCAA TATCATCTCG CCAGCATCAT 
CATGCCCATC ATTCTCATTC CAAAAAAGTT AATAAGAAGT TCGAGATTGC AAATGATATG 
TTTTGGAAAG ATGGTGAACC TTTTCGGATC ATCGGTGGTG ACTTGCATTA TTTTCGAGTG 
CATCCTGAGT ATTGGGAAGA TAGATTGTTA AAAGCCAAGG CATTGGGCTT GAACACAATT 
CAGACATATG TTCCTTGGAA TCTGCATGAA CCAGCACCAG AAAAGCTCGT TTTTGAGGGT 
ATTGCAAATA TAGAATCATT CCTCAACCTT TGTCACAAAC TCGATCTCCT CGTGATGGTT 
CGACCCGGAC CATATATATG TGCAGAGTGG GACTGGGGTG GTATCCCAAG TTGGTTATTT 
TCCAGGAATC CAACCCCAAA ACCAAGATCA TCTGATCCTG CTTTTCTTAA ACTGGTTGAA 
AGATGGTGGG GCAAACTGCT TCCAAAATTA GTTCCTCTCC TATATGACAA CGGAGGCCCT 
ATAATAATGG TTCAGATTGA GAATGAGTAT GGTTCTTATG GAGACGACAA AGCATATCTC 
CATCACCTGA TCACACTAGC TAGAGGTCAT CTTGGGCAGG ATGCCATTTT ATACACTACA 
GATGGAGGGT CCAGGGAAAA TCTTGAGAAA GGAACTATTC GTGGAGATAC TGTCTTTTCA 
GCTGTTGACT TCACTACTGG TGATGATCCT TGGCCTATAT TCAAGTTACA GAAGGAGTTC 
AATGCCCCAG GAAAATCGCC ACCTTTGTCA TCCGAATTTT ACACTGGGTG GCTTACACAT 
TGGGGAGAGA AAAATGCTAA GACCGATGCT GATTCCACAG CAGCTGCTCT TGAAGAAATT 
TTGAGAAAAA ATGGGTCTGC CGTCCTATAC ATGGCGCATG GTGGTTCAAA CTTTGGATTT 
TATAATGGAG CAAATACCGG TGCAAACGAG GCTGACTATA AACCTGATCT CACTTCATAT 
GATTATGATG CCCCGATCAG GGAAGCTGGT GATGTTGACA ATTCAAAATT CAATGCAATT 
CGGAGGGTTA TATCCCGATA TAGCTCTGCA CCTCTTCCTT CTATTCCCTC TTATAATGAA 
AAGACAACAT ATGGACCAAT CCACTTGCAA AGACGATCTT CTTTATTTGA TATATTTGAT 
TTCACCAATT CCTCCAGCTC CTTTGAATCA GAAAATCCAA TGTCAATGGA GAATGTGGGC 
CAGTTTTTTG GTTTCTTATT GTATGTCACT GATTACGAAG CAAGGAGAGG GGGAAGAAAT 
TTGTCCATAC CAAAGGTGCA TGATAGAGCT CAAGTATTTA TCTCATGTTC TTCTAAAGGA 
AGGGGTACAA GGCCAACTTA TGTTGGTACT GTCGAAAGAT GGTTGAATAA AAAACTCAGC 
CTCCCTGAAT ATCAATGCCA TTCTAAGATC AACTTATATA TCTTGGTTGA AAACATGGGA 
CGTGTAAATT ATGGGCCTTT CATCTTCGAT AGGAAGGGTA TTCTGTCTTC TGTTTATCTG 
GATGGGAATA GAGTACAAGG TTGGAAAATG TTCCCTATTC CTTTGCACAA TCTAAACGAA 
GTGCCAAATT ACAATCGCAT AATGCAAGCT TCATATTCTG CATTCGGTGA AATATCAACC 
TCCAGGAAAA GGTTGATGAA TAAGTCTGAG AATACTTCAA AAGAACCTGC TTTTTATTCT 
GGACACTTCT TAATTGATAA AACAAGTCAG GTCAAGGATA CATTTTTGTC ATTCAGAAAT 
TGGGGGAAAG GCGTTGTGTT TGTTAATGAC TTCAATCTAG GAAGATATTG GCCGTTGAGA 
GGACCACAGT GCAACCTCTA CGTTCCTGCT CCAGTCCTTA AGCAAGGAGA TAATTTTGTG 
GTAATATTCG AGTTGGAATC TCCAGACCCC AATCTTTTGG TGCACTCGGT TGATGAGCCA 
GACTACACAT GTGGCTTTAA TGGGATGAAC ATACATCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC11427149 ( XM_024776825.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024632593.1 Medicago truncatula beta-galactosidase 17 (LOC11427149), transcript variant X2, mRNA.

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CloneID	OMe260065
Clone ID Related Accession (Same CDS sequence)	XM_024776825.1
Accession Version	XM_024776825.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	beta-galactosidase 17 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016408.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGAAGA AACGAATTTC CACAGCTACC CTTTTCTTCA TCTTCATCTC TTTCATTGCT 
CTTTGTGCCA TTGTTCCTGT TTTTGCTCCT CTTCCTTCAA TATCATCTCG CCAGCATCAT 
CATGCCCATC ATTCTCATTC CAAAAAAGTT AATAAGAAGT TCGAGATTGC AAATGATATG 
TTTTGGAAAG ATGGTGAACC TTTTCGGATC ATCGGTGGTG ACTTGCATTA TTTTCGAGTG 
CATCCTGAGT ATTGGGAAGA TAGATTGTTA AAAGCCAAGG CATTGGGCTT GAACACAATT 
CAGACATATG TTCCTTGGAA TCTGCATGAA CCAGCACCAG AAAAGCTCGT TTTTGAGGGT 
ATTGCAAATA TAGAATCATT CCTCAACCTT TGTCACAAAC TCGATCTCCT CGTGATGGTT 
CGACCCGGAC CATATATATG TGCAGAGTGG GACTGGGGTG GTATCCCAAG TTGGTTATTT 
TCCAGGAATC CAACCCCAAA ACCAAGATCA TCTGATCCTG CTTTTCTTAA ACTGGTTGAA 
AGATGGTGGG GCAAACTGCT TCCAAAATTA GTTCCTCTCC TATATGACAA CGGAGGCCCT 
ATAATAATGG TTCAGATTGA GAATGAGTAT GGTTCTTATG GAGACGACAA AGCATATCTC 
CATCACCTGA TCACACTAGC TAGAGGTCAT CTTGGGCAGG ATGCCATTTT ATACACTACA 
GATGGAGGGT CCAGGGAAAA TCTTGAGAAA GGAACTATTC GTGGAGATAC TGTCTTTTCA 
GCTGTTGACT TCACTACTGG TGATGATCCT TGGCCTATAT TCAAGTTACA GAAGGAGTTC 
AATGCCCCAG GAAAATCGCC ACCTTTGTCA TCCGAATTTT ACACTGGGTG GCTTACACAT 
TGGGGAGAGA AAAATGCTAA GACCGATGCT GATTCCACAG CAGCTGCTCT TGAAGAAATT 
TTGAGAAAAA ATGGGTCTGC CGTCCTATAC ATGGCGCATG GTGGTTCAAA CTTTGGATTT 
TATAATGGAG CAAATACCGG TGCAAACGAG GCTGACTATA AACCTGATCT CACTTCATAT 
GATTATGATG CCCCGATCAG GGAAGCTGGT GATGTTGACA ATTCAAAATT CAATGCAATT 
CGGAGGGTTA TATCCCGATA TAGCTCTGCA CCTCTTCCTT CTATTCCCTC TTATAATGAA 
AAGACAACAT ATGGACCAAT CCACTTGCAA AGACGATCTT CTTTATTTGA TATATTTGAT 
TTCACCAATT CCTCCAGCTC CTTTGAATCA GAAAATCCAA TGTCAATGGA GAATGTGGGC 
CAGGTGCATG ATAGAGCTCA AGTATTTATC TCATGTTCTT CTAAAGGAAG GGGTACAAGG 
CCAACTTATG TTGGTACTGT CGAAAGATGG TTGAATAAAA AACTCAGCCT CCCTGAATAT 
CAATGCCATT CTAAGATCAA CTTATATATC TTGGTTGAAA ACATGGGACG TGTAAATTAT 
GGGCCTTTCA TCTTCGATAG GAAGGGTATT CTGTCTTCTG TTTATCTGGA TGGGAATAGA 
GTACAAGGTT GGAAAATGTT CCCTATTCCT TTGCACAATC TAAACGAAGT GCCAAATTAC 
AATCGCATAA TGCAAGCTTC ATATTCTGCA TTCGGTGAAA TATCAACCTC CAGGAAAAGG 
TTGATGAATA AGTCTGAGAA TACTTCAAAA GAACCTGCTT TTTATTCTGG ACACTTCTTA 
ATTGATAAAA CAAGTCAGGT CAAGGATACA TTTTTGTCAT TCAGAAATTG GGGGAAAGGC 
GTTGTGTTTG TTAATGACTT CAATCTAGGA AGATATTGGC CGTTGAGAGG ACCACAGTGC 
AACCTCTACG TTCCTGCTCC AGTCCTTAAG CAAGGAGATA ATTTTGTGGT AATATTCGAG 
TTGGAATCTC CAGACCCCAA TCTTTTGGTG CACTCGGTTG ATGAGCCAGA CTACACATGT 
GGCTTTAATG GGATGAACAT ACATCAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024632593.1
CDS	89..2101
Translation	MAKKRISTATLFFIFISFIALCAIVPVFAPLPSISSRQHHHAHHSHSKKVNKKFEIANDMFWKDGEPFRIIGGDLHYFRVHPEYWEDRLLKAKALGLNTIQTYVPWNLHEPAPEKLVFEGIANIESFLNLCHKLDLLVMVRPGPYICAEWDWGGIPSWLFSRNPTPKPRSSDPAFLKLVERWWGKLLPKLVPLLYDNGGPIIMVQIENEYGSYGDDKAYLHHLITLARGHLGQDAILYTTDGGSRENLEKGTIRGDTVFSAVDFTTGDDPWPIFKLQKEFNAPGKSPPLSSEFYTGWLTHWGEKNAKTDADSTAAALEEILRKNGSAVLYMAHGGSNFGFYNGANTGANEADYKPDLTSYDYDAPIREAGDVDNSKFNAIRRVISRYSSAPLPSIPSYNEKTTYGPIHLQRRSSLFDIFDFTNSSSSFESENPMSMENVGQVHDRAQVFISCSSKGRGTRPTYVGTVERWLNKKLSLPEYQCHSKINLYILVENMGRVNYGPFIFDRKGILSSVYLDGNRVQGWKMFPIPLHNLNEVPNYNRIMQASYSAFGEISTSRKRLMNKSENTSKEPAFYSGHFLIDKTSQVKDTFLSFRNWGKGVVFVNDFNLGRYWPLRGPQCNLYVPAPVLKQGDNFVVIFELESPDPNLLVHSVDEPDYTCGFNGMNIHQL

Target ORF information:

RefSeq Version	XM_024776825.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula beta-galactosidase 17 (LOC11427149), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024776825.1

ORF Insert Sequence:

ATGGCGAAGA AACGAATTTC CACAGCTACC CTTTTCTTCA TCTTCATCTC TTTCATTGCT 
CTTTGTGCCA TTGTTCCTGT TTTTGCTCCT CTTCCTTCAA TATCATCTCG CCAGCATCAT 
CATGCCCATC ATTCTCATTC CAAAAAAGTT AATAAGAAGT TCGAGATTGC AAATGATATG 
TTTTGGAAAG ATGGTGAACC TTTTCGGATC ATCGGTGGTG ACTTGCATTA TTTTCGAGTG 
CATCCTGAGT ATTGGGAAGA TAGATTGTTA AAAGCCAAGG CATTGGGCTT GAACACAATT 
CAGACATATG TTCCTTGGAA TCTGCATGAA CCAGCACCAG AAAAGCTCGT TTTTGAGGGT 
ATTGCAAATA TAGAATCATT CCTCAACCTT TGTCACAAAC TCGATCTCCT CGTGATGGTT 
CGACCCGGAC CATATATATG TGCAGAGTGG GACTGGGGTG GTATCCCAAG TTGGTTATTT 
TCCAGGAATC CAACCCCAAA ACCAAGATCA TCTGATCCTG CTTTTCTTAA ACTGGTTGAA 
AGATGGTGGG GCAAACTGCT TCCAAAATTA GTTCCTCTCC TATATGACAA CGGAGGCCCT 
ATAATAATGG TTCAGATTGA GAATGAGTAT GGTTCTTATG GAGACGACAA AGCATATCTC 
CATCACCTGA TCACACTAGC TAGAGGTCAT CTTGGGCAGG ATGCCATTTT ATACACTACA 
GATGGAGGGT CCAGGGAAAA TCTTGAGAAA GGAACTATTC GTGGAGATAC TGTCTTTTCA 
GCTGTTGACT TCACTACTGG TGATGATCCT TGGCCTATAT TCAAGTTACA GAAGGAGTTC 
AATGCCCCAG GAAAATCGCC ACCTTTGTCA TCCGAATTTT ACACTGGGTG GCTTACACAT 
TGGGGAGAGA AAAATGCTAA GACCGATGCT GATTCCACAG CAGCTGCTCT TGAAGAAATT 
TTGAGAAAAA ATGGGTCTGC CGTCCTATAC ATGGCGCATG GTGGTTCAAA CTTTGGATTT 
TATAATGGAG CAAATACCGG TGCAAACGAG GCTGACTATA AACCTGATCT CACTTCATAT 
GATTATGATG CCCCGATCAG GGAAGCTGGT GATGTTGACA ATTCAAAATT CAATGCAATT 
CGGAGGGTTA TATCCCGATA TAGCTCTGCA CCTCTTCCTT CTATTCCCTC TTATAATGAA 
AAGACAACAT ATGGACCAAT CCACTTGCAA AGACGATCTT CTTTATTTGA TATATTTGAT 
TTCACCAATT CCTCCAGCTC CTTTGAATCA GAAAATCCAA TGTCAATGGA GAATGTGGGC 
CAGGTGCATG ATAGAGCTCA AGTATTTATC TCATGTTCTT CTAAAGGAAG GGGTACAAGG 
CCAACTTATG TTGGTACTGT CGAAAGATGG TTGAATAAAA AACTCAGCCT CCCTGAATAT 
CAATGCCATT CTAAGATCAA CTTATATATC TTGGTTGAAA ACATGGGACG TGTAAATTAT 
GGGCCTTTCA TCTTCGATAG GAAGGGTATT CTGTCTTCTG TTTATCTGGA TGGGAATAGA 
GTACAAGGTT GGAAAATGTT CCCTATTCCT TTGCACAATC TAAACGAAGT GCCAAATTAC 
AATCGCATAA TGCAAGCTTC ATATTCTGCA TTCGGTGAAA TATCAACCTC CAGGAAAAGG 
TTGATGAATA AGTCTGAGAA TACTTCAAAA GAACCTGCTT TTTATTCTGG ACACTTCTTA 
ATTGATAAAA CAAGTCAGGT CAAGGATACA TTTTTGTCAT TCAGAAATTG GGGGAAAGGC 
GTTGTGTTTG TTAATGACTT CAATCTAGGA AGATATTGGC CGTTGAGAGG ACCACAGTGC 
AACCTCTACG TTCCTGCTCC AGTCCTTAAG CAAGGAGATA ATTTTGTGGT AATATTCGAG 
TTGGAATCTC CAGACCCCAA TCTTTTGGTG CACTCGGTTG ATGAGCCAGA CTACACATGT 
GGCTTTAATG GGATGAACAT ACATCAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC11427149 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム