MTR_8g052060 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTR_8g052060
Entrez Gene ID	11409308
Full Name	cysteine-rich RLK (receptor-like kinase) protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024772943.1	XP_024628711.1	uncharacterized protein LOC11409308
XM_003628254.2	XP_003628302.2	cysteine-rich RLK (receptor-like kinase) protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MTR_8g052060 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTR_8g052060 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe161093	XM_003628254.2 Latest version!	Medicago truncatula cysteine-rich RLK (receptor-like kinase) protein partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OMe269527	XM_024772943.1 Latest version!	Medicago truncatula uncharacterized LOC11409308 (LOC11409308), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTR_8g052060 ( XM_003628254.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_003628302.2 Medicago truncatula cysteine-rich RLK (receptor-like kinase) protein partial mRNA.

Price & Availability↑

CloneID	OMe161093
Clone ID Related Accession (Same CDS sequence)	XM_003628254.2
Accession Version	XM_003628254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	cysteine-rich RLK (receptor-like kinase) protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016414). On Aug 25, 2015 this sequence version replaced XM_003628254.1. ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on the 5' end.

ATGATAAGCT TTAAGATAAA GAAACAAGTT GTATTGATAT ACTTATGGTT GTGGTGGATT 
ACTTCTACTA ACATTTGTGT TAATGCAACA AATGATAGTT TGAAGCCAGG TGATACATTG 
AATTCCAAAT CCAAGCTATG TTCTAAACAA GGCAAGTATT GTCTATATTT CAATCGAACA 
TTAGATAGCG AAGATGCTCA CTTGGTCATT GGTGTTAATG CCGAGTATGG CGCAGTAGTG 
TGGATGAAAC CAATAATCAT TTACTCGTCA CCTCAACCCA TAAACAACAC TTTGGCCACT 
ATATTAGACA CAGGTAACTT TGTGCTTCAA CAATTTCACC CAAATGGGAC AAATAGTTTA 
TTATGGCAGA GTTTTGATTA TCCCGATCAC ACTTTGATCC CAACGATGAA GTTGGGAGTT 
AATCGAAAAA CTGGACATAA TTGGTCATTG GTTTCATGGA TGACTCCTTC GCTACCAACT 
CCAGGTGAAT TTAGTCTTGA ATGGGAACCA AAAGAAGGTG AATTGAACAT CAAGAAAAGC 
GGCATAGCGT ATTGGAAAAG CGGAAAACTT AATAGCAATG GAATATTCGA GAACATTCCC 
ACGAAAGTTC AACGTATATA TCAGTACATT ATTGTCTCTA ACAAGAATGA AGATTCTTTC 
GCTTTCGAAG TTAAAGATGG GAAGTTTGCA CGATGGCAAC TAACCTCGAA CGGGAGGTTA 
GTAGGTCATG ATGGAGATAT TGGGAATGCA GATATGTGTT ATGGATATAA TAGCAATGGA 
GGGTGCCAAA AATGGGAGGA AATACCAAAT TGTAGGGAAA ATGGTGAGGT TTTTCAGAAA 
ATGGTGGGAA CACCAACCTT AGACTATGAA ACAGTGTTTG AATTCGATGT GACTTATAGT 
TACAGTGATT GCAAGATTAG ATGTTGGAGA AATTGTTATT GCAATGGATT CCAGGAATTT 
TATGGTAATG GGACTGGATG TACATTCTAT TCATGGAATT CAACACAATA TGTTGATTTG 
GTGAGTCAGA ATAACTTCTA CGTCTTGGTA AACTCTATAA AATCAGCCCC AAATTCCCAT 
GGTAAAAAAA AGTGGATATG GATAACTTCT ACAATCGCAG CTGCTTTACT CATATTTTGC 
CCAATCATTT TATGCTTAGC CAAAAAGAAA CAAAAATATG CACTTCAAGA TAAGAAAAGT 
AAAAGAAAGG ATTTGGCGGA TTCGACTGAA TCCTATAATA TCAAAGATCT TGAACATGAC 
TTCAAGGAAC ATGATATAAA AGTTTTTAAC TTTACATCAA TTTTGGAAGC AACTATGGAC 
TTTTCACCTA AAAACAAGTT AGGACAGGGC GGCTATGGAC CCATTTATAA GGGAATCTTG 
GCAACCGGGC AAGAAGTTGC TGTAAAAGGA CTTTCAAAAA CATCCGGACA AGGAATTGTA 
GAATTCAAAA ATGAATTGGT CCTTATATGT GAACTTCAGC ACAGAAATCT TGTAGAACTA 
CTTGGTTGTT GCATTCATGA AGAAGAGAGG ATTCTAATTT ACGAATATAT GTCAAACAAA 
AGCTTGGATT TCTACCTATT TGAAGATTGT ACAAAAAAGA AGCTGCTAGA TTGGAAGAAG 
CGTTTTAACA TAATAGAAGG AATTGCTCAA GGATTGCTCT ATCTTCATAA GTACTCAAGA 
CTCAAAATTA TTCATAGAGA CTTGAAGGCG AGCAACATAC TTCTTGATGA GAATATGAAC 
CCAAAAATTT CTGATTTTGG TATGGCAAGA ATGTTCACAC AACAGGAATC CGTAGTAAAT 
ACCAACAGGA TTGTTGGGAC ATATGGATAC ATGTCTCCAG AATATGCCAT GGAAGGAGTT 
TGTTCTACAA AGTCTGACGT CTATAGCTTT GGAGTGTTGC TACTTGAAAT CGTTTGTGGG 
AGAAAAAACA ATAGCTTCTA TGACGTTGAT CGCCCACTAA ATCTAATAGG ACATGCATGG 
GAGCTATGGA ATGATGGTGA GTATCTACAG TTAATGGATC CAACATTAAA TGACACATTT 
GTTCCTGATG AAGTGAAAAG GTGCATTCAT GTTGGTCTCT TATGTGTAGA GCAATATGCA 
AATGATCGAC CAACAATGTC TGATGTTATA TCTGTGTTAA CAAACAAATA CCAACTCACA 
AACTTACCAA GAAGACCAGC ATTTTATGTT AGAAGAGAGA TCTTTGAGGG GGAAACAATT 
TCCAAAGGGC AAGACACCGA CACCTATTCT ACGACAGCAA TTTCTACTTC ATGTGAAGAA 
GGGAAAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003628302.2
CDS	1..2292
Translation	MISFKIKKQVVLIYLWLWWITSTNICVNATNDSLKPGDTLNSKSKLCSKQGKYCLYFNRTLDSEDAHLVIGVNAEYGAVVWMKPIIIYSSPQPINNTLATILDTGNFVLQQFHPNGTNSLLWQSFDYPDHTLIPTMKLGVNRKTGHNWSLVSWMTPSLPTPGEFSLEWEPKEGELNIKKSGIAYWKSGKLNSNGIFENIPTKVQRIYQYIIVSNKNEDSFAFEVKDGKFARWQLTSNGRLVGHDGDIGNADMCYGYNSNGGCQKWEEIPNCRENGEVFQKMVGTPTLDYETVFEFDVTYSYSDCKIRCWRNCYCNGFQEFYGNGTGCTFYSWNSTQYVDLVSQNNFYVLVNSIKSAPNSHGKKKWIWITSTIAAALLIFCPIILCLAKKKQKYALQDKKSKRKDLADSTESYNIKDLEHDFKEHDIKVFNFTSILEATMDFSPKNKLGQGGYGPIYKGILATGQEVAVKGLSKTSGQGIVEFKNELVLICELQHRNLVELLGCCIHEEERILIYEYMSNKSLDFYLFEDCTKKKLLDWKKRFNIIEGIAQGLLYLHKYSRLKIIHRDLKASNILLDENMNPKISDFGMARMFTQQESVVNTNRIVGTYGYMSPEYAMEGVCSTKSDVYSFGVLLLEIVCGRKNNSFYDVDRPLNLIGHAWELWNDGEYLQLMDPTLNDTFVPDEVKRCIHVGLLCVEQYANDRPTMSDVISVLTNKYQLTNLPRRPAFYVRREIFEGETISKGQDTDTYSTTAISTSCEEGKI

Target ORF information:

RefSeq Version	XM_003628254.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula cysteine-rich RLK (receptor-like kinase) protein partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003628254.2

ORF Insert Sequence:

ATGATAAGCT TTAAGATAAA GAAACAAGTT GTATTGATAT ACTTATGGTT GTGGTGGATT 
ACTTCTACTA ACATTTGTGT TAATGCAACA AATGATAGTT TGAAGCCAGG TGATACATTG 
AATTCCAAAT CCAAGCTATG TTCTAAACAA GGCAAGTATT GTCTATATTT CAATCGAACA 
TTAGATAGCG AAGATGCTCA CTTGGTCATT GGTGTTAATG CCGAGTATGG CGCAGTAGTG 
TGGATGAAAC CAATAATCAT TTACTCGTCA CCTCAACCCA TAAACAACAC TTTGGCCACT 
ATATTAGACA CAGGTAACTT TGTGCTTCAA CAATTTCACC CAAATGGGAC AAATAGTTTA 
TTATGGCAGA GTTTTGATTA TCCCGATCAC ACTTTGATCC CAACGATGAA GTTGGGAGTT 
AATCGAAAAA CTGGACATAA TTGGTCATTG GTTTCATGGA TGACTCCTTC GCTACCAACT 
CCAGGTGAAT TTAGTCTTGA ATGGGAACCA AAAGAAGGTG AATTGAACAT CAAGAAAAGC 
GGCATAGCGT ATTGGAAAAG CGGAAAACTT AATAGCAATG GAATATTCGA GAACATTCCC 
ACGAAAGTTC AACGTATATA TCAGTACATT ATTGTCTCTA ACAAGAATGA AGATTCTTTC 
GCTTTCGAAG TTAAAGATGG GAAGTTTGCA CGATGGCAAC TAACCTCGAA CGGGAGGTTA 
GTAGGTCATG ATGGAGATAT TGGGAATGCA GATATGTGTT ATGGATATAA TAGCAATGGA 
GGGTGCCAAA AATGGGAGGA AATACCAAAT TGTAGGGAAA ATGGTGAGGT TTTTCAGAAA 
ATGGTGGGAA CACCAACCTT AGACTATGAA ACAGTGTTTG AATTCGATGT GACTTATAGT 
TACAGTGATT GCAAGATTAG ATGTTGGAGA AATTGTTATT GCAATGGATT CCAGGAATTT 
TATGGTAATG GGACTGGATG TACATTCTAT TCATGGAATT CAACACAATA TGTTGATTTG 
GTGAGTCAGA ATAACTTCTA CGTCTTGGTA AACTCTATAA AATCAGCCCC AAATTCCCAT 
GGTAAAAAAA AGTGGATATG GATAACTTCT ACAATCGCAG CTGCTTTACT CATATTTTGC 
CCAATCATTT TATGCTTAGC CAAAAAGAAA CAAAAATATG CACTTCAAGA TAAGAAAAGT 
AAAAGAAAGG ATTTGGCGGA TTCGACTGAA TCCTATAATA TCAAAGATCT TGAACATGAC 
TTCAAGGAAC ATGATATAAA AGTTTTTAAC TTTACATCAA TTTTGGAAGC AACTATGGAC 
TTTTCACCTA AAAACAAGTT AGGACAGGGC GGCTATGGAC CCATTTATAA GGGAATCTTG 
GCAACCGGGC AAGAAGTTGC TGTAAAAGGA CTTTCAAAAA CATCCGGACA AGGAATTGTA 
GAATTCAAAA ATGAATTGGT CCTTATATGT GAACTTCAGC ACAGAAATCT TGTAGAACTA 
CTTGGTTGTT GCATTCATGA AGAAGAGAGG ATTCTAATTT ACGAATATAT GTCAAACAAA 
AGCTTGGATT TCTACCTATT TGAAGATTGT ACAAAAAAGA AGCTGCTAGA TTGGAAGAAG 
CGTTTTAACA TAATAGAAGG AATTGCTCAA GGATTGCTCT ATCTTCATAA GTACTCAAGA 
CTCAAAATTA TTCATAGAGA CTTGAAGGCG AGCAACATAC TTCTTGATGA GAATATGAAC 
CCAAAAATTT CTGATTTTGG TATGGCAAGA ATGTTCACAC AACAGGAATC CGTAGTAAAT 
ACCAACAGGA TTGTTGGGAC ATATGGATAC ATGTCTCCAG AATATGCCAT GGAAGGAGTT 
TGTTCTACAA AGTCTGACGT CTATAGCTTT GGAGTGTTGC TACTTGAAAT CGTTTGTGGG 
AGAAAAAACA ATAGCTTCTA TGACGTTGAT CGCCCACTAA ATCTAATAGG ACATGCATGG 
GAGCTATGGA ATGATGGTGA GTATCTACAG TTAATGGATC CAACATTAAA TGACACATTT 
GTTCCTGATG AAGTGAAAAG GTGCATTCAT GTTGGTCTCT TATGTGTAGA GCAATATGCA 
AATGATCGAC CAACAATGTC TGATGTTATA TCTGTGTTAA CAAACAAATA CCAACTCACA 
AACTTACCAA GAAGACCAGC ATTTTATGTT AGAAGAGAGA TCTTTGAGGG GGAAACAATT 
TCCAAAGGGC AAGACACCGA CACCTATTCT ACGACAGCAA TTTCTACTTC ATGTGAAGAA 
GGGAAAATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTR_8g052060 ( XM_024772943.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_024628711.1 Medicago truncatula uncharacterized LOC11409308 (LOC11409308), mRNA.

Price & Availability↑

CloneID	OMe269527
Clone ID Related Accession (Same CDS sequence)	XM_024772943.1
Accession Version	XM_024772943.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4626bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	uncharacterized protein LOC11409308
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016414.2) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGATAAGCT TTAAAAGAAA TAAACAAATT GTTTGGATAT ACTTATGGTT GTGGTGGAAC 
ACTACTGCTA ATATTTGTGT TAAAGCAACA AGTGATAGTT TGAAGCCAGG TGATACATTA 
AATTCCAAAT CCAAGCTATG TTCTGAACAA GGAAAGTTTT GTCTATATTT CGATAGCGAA 
GAAGCTCACT TGGTTGTAAG TAGTGGTGTT GACGGCGCAG TAGTGTGGAT GTACGATAGA 
AATCAACCCA TAGCCATAGA TTCAGCAGTT CTATCACTAG ACTACTCTGG TGTACTCAAA 
ATAGAATTTC AAAATAGGAA TGTACCAATT ATCATTTATT ATTCGCCTCA ACCAACCAAC 
GACACTGTGG CCACTATGTT AGACACCGGT AACTTCGTGC TTCAACAGCT TCACCCAAAT 
GGGACAAAGA GTATATTATG GCAGAGTTTT GATTCTCCCG TAGACACTTT GTTACCAACG 
ATGAAGTTAG GTGTTAATCG TAAAACAGGA CATAATTGGT CATTGGTTTC ACGGTTGGCT 
CATTCGCTAC CAACTCCAGG TGAACTTAGT CTTGAATGGG AACCAAAAGA AGGTGAATTG 
AACATCAGGA AAAGTGGCAA AGTGCATTGG AAAAGTGGAA AACTCAAAAG CAATGGAATG 
TTCGAGAACA TTCCTGCGAA AGTTCAACGT ATATACCAGT ACATTATTGT GTCTAACAAG 
GATGAAGATT CTTTTGCTTT TGAAGTTAAA GATGGGAAAT TTATACGATG GTTCATATCC 
CCGAAGGGGA GGTTAATAAG TGATGCTGGA AGCACTTCTA ATGCAGACAT GTGTTATGGA 
TATAAAAGTG ATGAAGGGTG CCAAGTTGCA AATGCAGACA TGTGTTATGG ATATAATAGC 
GATGGAGGGT GCCAAAAATG GGAGGAAATA CCAAATTGTA GGGAACCTGG TGAGGTTTTT 
CGGAAAATGG TGGGAAGACC AAATAAAGAC AATGCAACAA CAGATGAACC TGCTAATGGT 
TACGATGATT GCAAGATGAG ATGTTGGAGA AATTGTAATT GCTATGGATT CGAAGAATTA 
TACAGTAATT TTACTGGATG TATATACTAT TCATGGAATT CGACACAAGA CGTTGATTTG 
GACAAAAAGA ATAACTTTTA CGCCTTGGTA AAGCCAACAA AATCACCTCC GAATTCCCAT 
GTCAATGCAG GTAAGAGGAG GATATGGATA GGTGCTGCAA TCGCAACTGC TTTACTCATA 
TTATGCCCAC TCATTTTATT CCTAGCCAAA AAGAAACAAA AATATGCACT ACAAGGTAAG 
AAAAGTAAAA GAAAGGAGGG TAAGATGAAA GATTTGGCGG AATCCTATGA TATCAAAGAT 
CTTGAAAATG ACTTCAAAGG ACATGATATA AAAGTTTTTA ACTTTACATC AATTTTGGAA 
GCAACTATGG ATTTTTCATC TGAAAACAAG TTAGGACAGG GAGGCTATGG ACCCGTTTAT 
AAGGGAATCT TGGCAACAGG ACAAGAAGTT GCTGTAAAAA GACTTTCAAA AACATCTGGA 
CAAGGAATTG TGGAATTCAG AAATGAATTG GCATTGATAT GTGAACTTCA ACACACAAAT 
CTTGTACAAC TACTTGGTTG TTGCATTCAT GAAGAAGAAA GGATTCTAAT TTACGAATAT 
ATGCCAAATA AAAGCTTGGA TTTCTATCTA TTTGATTGTA CAAGAAAAAA GTTACTAGAC 
TGGAAGAAGC GCCTGAACAT AATCGAAGGA ATTTCTCAAG GATTACTCTA TCTTCATAAG 
TACTCTAGAC TCAAAATCAT TCATAGAGAC TTGAAGGCTA GCAACATACT CCTTGATGAG 
AATTTGAACC CGAAAATTTC AGATTTCGGT ATGGCAAGAA TGTTCACGCA ACAAGAATCA 
ATAGTTAACA CCAACAGGAT TGTTGGGACA TATGGATACA TGTCTCCAGA ATATGCCATG 
GAAGGAATTT GTTCAACAAA GTCTGATGTT TATAGCTTTG GAGTGTTGCT TCTTGAAATT 
ATTTGTGGAA GAAAAAACAA TAGCTTCCAT GATGTTGATC GCCCACTAAA CCTAATAGGA 
CATGCATGGG AGCTATGGAA TGATGGTGAG TATCTACAGT TACTGGATCC ATCTTTATGT 
GACACATTTG TTCCTGATGA AGTGCAAAGG TGCATTCATG TTGGTCTCTT ATGTGTACAA 
CAATATGCTA ATGATCGACC TACAATGTCC GATGTTATAT CAATGTTAAC AAACAAATAT 
AAACTCACAA CTTTACCAAG AAGACCGGCA TTCTATATTA GAAGAGAGAT CTATGACGGG 
GAAACAACTT CTAAAGGGCC AGACACCGAC ACCTATTCTA CGACAGCAAT TTCTACCTCA 
TGTGAAGTAG AAGGAAAATA TAAGTATTTG AAGCCAGGTG ATACATTGAA TTCCAAATCC 
AAGCTATGTT CTAAACAAGG CAAGTATTGT CTATATTTCA ATCGAACATT AGATAGCGAA 
GATGCTCACT TGGTCATTGG TGTTAATGCC GAGTATGGCG CAGTAGTGTG GATGAAACCA 
ATAATCATTT ACTCGTCACC TCAACCCATA AACAACACTT TGGCCACTAT ATTAGACACA 
GGTAACTTTG TGCTTCAACA ATTTCACCCA AATGGGACAA ATAGTTTATT ATGGCAGAGT 
TTTGATTATC CCGATCACAC TTTGATCCCA ACGATGAAGT TGGGAGTTAA TCGAAAAACT 
GGACATAATT GGTCATTGGT TTCATGGATG ACTCCTTCGC TACCAACTCC AGGTGAATTT 
AGTCTTGAAT GGGAACCAAA AGAAGGTGAA TTGAACATCA AGAAAAGCGG CATAGCGTAT 
TGGAAAAGCG GAAAACTTAA TAGCAATGGA ATATTCGAGA ACATTCCCAC GAAAGTTCAA 
CGTATATATC AGTACATTAT TGTCTCTAAC AAGAATGAAG ATTCTTTCGC TTTCGAAGTT 
AAAGATGGGA AGTTTGCACG ATGGCAACTA ACCTCGAACG GGAGGTTAGT AGGTCATGAT 
GGAGATATTG GGAATGCAGA TATGTGTTAT GGATATAATA GCAATGGAGG GTGCCAAAAA 
TGGGAGGAAA TACCAAATTG TAGGGAAAAT GGTGAGGTTT TTCAGAAAAT GGTGGGAACA 
CCAACCTTAG ACTATGAAAC AGTGTTTGAA TTCGATGTGA CTTATAGTTA CAGTGATTGC 
AAGATTAGAT GTTGGAGAAA TTGTTATTGC AATGGATTCC AGGAATTTTA TGGTAATGGG 
ACTGGATGTA CATTCTATTC ATGGAATTCA ACACAATATG TTGATTTGGT GAGTCAGAAT 
AACTTCTACG TCTTGGTAAA CTCTATAAAA TCAGCCCCAA ATTCCCATGT CAATGCAGGT 
AAAAAAAAGT GGATATGGAT AACTTCTACA ATCGCAGCTG CTTTACTCAT ATTTTGCCCA 
ATCATTTTAT GCTTAGCCAA AAAGAAACAA AAATATGCAC TTCAAGATAA GAAAAGTAAA 
AGAAAGGATT TGGCGGATTC GACTGAATCC TATAATATCA AAGATCTTGA ACATGACTTC 
AAGGAACATG ATATAAAAGT TTTTAACTTT ACATCAATTT TGGAAGCAAC TATGGACTTT 
TCACCTAAAA ACAAGTTAGG ACAGGGCGGC TATGGACCCA TTTATAAGGG AATCTTGGCA 
ACCGGGCAAG AAGTTGCTGT AAAAGGACTT TCAAAAACAT CCGGACAAGG AATTGTAGAA 
TTCAAAAATG AATTGGTCCT TATATGTGAA CTTCAGCACA GAAATCTTGT AGAACTACTT 
GGTTGTTGCA TTCATGAAGA AGAGAGGATT CTAATTTACG AATATATGTC AAACAAAAGC 
TTGGATTTCT ACCTATTTGA TTGTACAAAA AAGAAGCTGC TAGATTGGAA GAAGCGTTTT 
AACATAATAG AAGGAATTGC TCAAGGATTG CTCTATCTTC ATAAGTACTC AAGACTCAAA 
ATTATTCATA GAGACTTGAA GGCGAGCAAC ATACTTCTTG ATGAGAATAT GAACCCAAAA 
ATTTCTGATT TTGGTATGGC AAGAATGTTC ACACAACAGG AATCCGTAGT AAATACCAAC 
AGGATTGTTG GGACATATGG ATACATGTCT CCAGAATATG CCATGGAAGG AGTTTGTTCT 
ACAAAGTCTG ACGTCTATAG CTTTGGAGTG TTGCTACTTG AAATCGTTTG TGGGAGAAAA 
AACAATAGCT TCTATGACGT TGATCGCCCA CTAAATCTAA TAGGACATGC ATGGGAGCTA 
TGGAATGATG GTGAGTATCT ACAGTTAATG GATCCAACAT TAAATGACAC ATTTGTTCCT 
GATGAAGTGA AAAGGTGCAT TCATGTTGGT CTCTTATGTG TAGAGCAATA TGCAAATGAT 
CGACCAACAA TGTCTGATGT TATATCTGTG TTAACAAACA AATACCAACT CACAAACTTA 
CCAAGAAGAC CAGCATTTTA TGTTAGAAGA GAGATCTTTG AGGGGGAAAC AATTTCCAAA 
GGGCAAGACA CCGACACCTA TTCTACGACA GCAATTTCTA CTTCATGTGA AGAAGGGAAA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024628711.1
CDS	253..4878
Translation	MISFKRNKQIVWIYLWLWWNTTANICVKATSDSLKPGDTLNSKSKLCSEQGKFCLYFDSEEAHLVVSSGVDGAVVWMYDRNQPIAIDSAVLSLDYSGVLKIEFQNRNVPIIIYYSPQPTNDTVATMLDTGNFVLQQLHPNGTKSILWQSFDSPVDTLLPTMKLGVNRKTGHNWSLVSRLAHSLPTPGELSLEWEPKEGELNIRKSGKVHWKSGKLKSNGMFENIPAKVQRIYQYIIVSNKDEDSFAFEVKDGKFIRWFISPKGRLISDAGSTSNADMCYGYKSDEGCQVANADMCYGYNSDGGCQKWEEIPNCREPGEVFRKMVGRPNKDNATTDEPANGYDDCKMRCWRNCNCYGFEELYSNFTGCIYYSWNSTQDVDLDKKNNFYALVKPTKSPPNSHVNAGKRRIWIGAAIATALLILCPLILFLAKKKQKYALQGKKSKRKEGKMKDLAESYDIKDLENDFKGHDIKVFNFTSILEATMDFSSENKLGQGGYGPVYKGILATGQEVAVKRLSKTSGQGIVEFRNELALICELQHTNLVQLLGCCIHEEERILIYEYMPNKSLDFYLFDCTRKKLLDWKKRLNIIEGISQGLLYLHKYSRLKIIHRDLKASNILLDENLNPKISDFGMARMFTQQESIVNTNRIVGTYGYMSPEYAMEGICSTKSDVYSFGVLLLEIICGRKNNSFHDVDRPLNLIGHAWELWNDGEYLQLLDPSLCDTFVPDEVQRCIHVGLLCVQQYANDRPTMSDVISMLTNKYKLTTLPRRPAFYIRREIYDGETTSKGPDTDTYSTTAISTSCEVEGKYKYLKPGDTLNSKSKLCSKQGKYCLYFNRTLDSEDAHLVIGVNAEYGAVVWMKPIIIYSSPQPINNTLATILDTGNFVLQQFHPNGTNSLLWQSFDYPDHTLIPTMKLGVNRKTGHNWSLVSWMTPSLPTPGEFSLEWEPKEGELNIKKSGIAYWKSGKLNSNGIFENIPTKVQRIYQYIIVSNKNEDSFAFEVKDGKFARWQLTSNGRLVGHDGDIGNADMCYGYNSNGGCQKWEEIPNCRENGEVFQKMVGTPTLDYETVFEFDVTYSYSDCKIRCWRNCYCNGFQEFYGNGTGCTFYSWNSTQYVDLVSQNNFYVLVNSIKSAPNSHVNAGKKKWIWITSTIAAALLIFCPIILCLAKKKQKYALQDKKSKRKDLADSTESYNIKDLEHDFKEHDIKVFNFTSILEATMDFSPKNKLGQGGYGPIYKGILATGQEVAVKGLSKTSGQGIVEFKNELVLICELQHRNLVELLGCCIHEEERILIYEYMSNKSLDFYLFDCTKKKLLDWKKRFNIIEGIAQGLLYLHKYSRLKIIHRDLKASNILLDENMNPKISDFGMARMFTQQESVVNTNRIVGTYGYMSPEYAMEGVCSTKSDVYSFGVLLLEIVCGRKNNSFYDVDRPLNLIGHAWELWNDGEYLQLMDPTLNDTFVPDEVKRCIHVGLLCVEQYANDRPTMSDVISVLTNKYQLTNLPRRPAFYVRREIFEGETISKGQDTDTYSTTAISTSCEEGKI

Target ORF information:

RefSeq Version	XM_024772943.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula uncharacterized LOC11409308 (LOC11409308), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024772943.1

ORF Insert Sequence:

ATGATAAGCT TTAAAAGAAA TAAACAAATT GTTTGGATAT ACTTATGGTT GTGGTGGAAC 
ACTACTGCTA ATATTTGTGT TAAAGCAACA AGTGATAGTT TGAAGCCAGG TGATACATTA 
AATTCCAAAT CCAAGCTATG TTCTGAACAA GGAAAGTTTT GTCTATATTT CGATAGCGAA 
GAAGCTCACT TGGTTGTAAG TAGTGGTGTT GACGGCGCAG TAGTGTGGAT GTACGATAGA 
AATCAACCCA TAGCCATAGA TTCAGCAGTT CTATCACTAG ACTACTCTGG TGTACTCAAA 
ATAGAATTTC AAAATAGGAA TGTACCAATT ATCATTTATT ATTCGCCTCA ACCAACCAAC 
GACACTGTGG CCACTATGTT AGACACCGGT AACTTCGTGC TTCAACAGCT TCACCCAAAT 
GGGACAAAGA GTATATTATG GCAGAGTTTT GATTCTCCCG TAGACACTTT GTTACCAACG 
ATGAAGTTAG GTGTTAATCG TAAAACAGGA CATAATTGGT CATTGGTTTC ACGGTTGGCT 
CATTCGCTAC CAACTCCAGG TGAACTTAGT CTTGAATGGG AACCAAAAGA AGGTGAATTG 
AACATCAGGA AAAGTGGCAA AGTGCATTGG AAAAGTGGAA AACTCAAAAG CAATGGAATG 
TTCGAGAACA TTCCTGCGAA AGTTCAACGT ATATACCAGT ACATTATTGT GTCTAACAAG 
GATGAAGATT CTTTTGCTTT TGAAGTTAAA GATGGGAAAT TTATACGATG GTTCATATCC 
CCGAAGGGGA GGTTAATAAG TGATGCTGGA AGCACTTCTA ATGCAGACAT GTGTTATGGA 
TATAAAAGTG ATGAAGGGTG CCAAGTTGCA AATGCAGACA TGTGTTATGG ATATAATAGC 
GATGGAGGGT GCCAAAAATG GGAGGAAATA CCAAATTGTA GGGAACCTGG TGAGGTTTTT 
CGGAAAATGG TGGGAAGACC AAATAAAGAC AATGCAACAA CAGATGAACC TGCTAATGGT 
TACGATGATT GCAAGATGAG ATGTTGGAGA AATTGTAATT GCTATGGATT CGAAGAATTA 
TACAGTAATT TTACTGGATG TATATACTAT TCATGGAATT CGACACAAGA CGTTGATTTG 
GACAAAAAGA ATAACTTTTA CGCCTTGGTA AAGCCAACAA AATCACCTCC GAATTCCCAT 
GTCAATGCAG GTAAGAGGAG GATATGGATA GGTGCTGCAA TCGCAACTGC TTTACTCATA 
TTATGCCCAC TCATTTTATT CCTAGCCAAA AAGAAACAAA AATATGCACT ACAAGGTAAG 
AAAAGTAAAA GAAAGGAGGG TAAGATGAAA GATTTGGCGG AATCCTATGA TATCAAAGAT 
CTTGAAAATG ACTTCAAAGG ACATGATATA AAAGTTTTTA ACTTTACATC AATTTTGGAA 
GCAACTATGG ATTTTTCATC TGAAAACAAG TTAGGACAGG GAGGCTATGG ACCCGTTTAT 
AAGGGAATCT TGGCAACAGG ACAAGAAGTT GCTGTAAAAA GACTTTCAAA AACATCTGGA 
CAAGGAATTG TGGAATTCAG AAATGAATTG GCATTGATAT GTGAACTTCA ACACACAAAT 
CTTGTACAAC TACTTGGTTG TTGCATTCAT GAAGAAGAAA GGATTCTAAT TTACGAATAT 
ATGCCAAATA AAAGCTTGGA TTTCTATCTA TTTGATTGTA CAAGAAAAAA GTTACTAGAC 
TGGAAGAAGC GCCTGAACAT AATCGAAGGA ATTTCTCAAG GATTACTCTA TCTTCATAAG 
TACTCTAGAC TCAAAATCAT TCATAGAGAC TTGAAGGCTA GCAACATACT CCTTGATGAG 
AATTTGAACC CGAAAATTTC AGATTTCGGT ATGGCAAGAA TGTTCACGCA ACAAGAATCA 
ATAGTTAACA CCAACAGGAT TGTTGGGACA TATGGATACA TGTCTCCAGA ATATGCCATG 
GAAGGAATTT GTTCAACAAA GTCTGATGTT TATAGCTTTG GAGTGTTGCT TCTTGAAATT 
ATTTGTGGAA GAAAAAACAA TAGCTTCCAT GATGTTGATC GCCCACTAAA CCTAATAGGA 
CATGCATGGG AGCTATGGAA TGATGGTGAG TATCTACAGT TACTGGATCC ATCTTTATGT 
GACACATTTG TTCCTGATGA AGTGCAAAGG TGCATTCATG TTGGTCTCTT ATGTGTACAA 
CAATATGCTA ATGATCGACC TACAATGTCC GATGTTATAT CAATGTTAAC AAACAAATAT 
AAACTCACAA CTTTACCAAG AAGACCGGCA TTCTATATTA GAAGAGAGAT CTATGACGGG 
GAAACAACTT CTAAAGGGCC AGACACCGAC ACCTATTCTA CGACAGCAAT TTCTACCTCA 
TGTGAAGTAG AAGGAAAATA TAAGTATTTG AAGCCAGGTG ATACATTGAA TTCCAAATCC 
AAGCTATGTT CTAAACAAGG CAAGTATTGT CTATATTTCA ATCGAACATT AGATAGCGAA 
GATGCTCACT TGGTCATTGG TGTTAATGCC GAGTATGGCG CAGTAGTGTG GATGAAACCA 
ATAATCATTT ACTCGTCACC TCAACCCATA AACAACACTT TGGCCACTAT ATTAGACACA 
GGTAACTTTG TGCTTCAACA ATTTCACCCA AATGGGACAA ATAGTTTATT ATGGCAGAGT 
TTTGATTATC CCGATCACAC TTTGATCCCA ACGATGAAGT TGGGAGTTAA TCGAAAAACT 
GGACATAATT GGTCATTGGT TTCATGGATG ACTCCTTCGC TACCAACTCC AGGTGAATTT 
AGTCTTGAAT GGGAACCAAA AGAAGGTGAA TTGAACATCA AGAAAAGCGG CATAGCGTAT 
TGGAAAAGCG GAAAACTTAA TAGCAATGGA ATATTCGAGA ACATTCCCAC GAAAGTTCAA 
CGTATATATC AGTACATTAT TGTCTCTAAC AAGAATGAAG ATTCTTTCGC TTTCGAAGTT 
AAAGATGGGA AGTTTGCACG ATGGCAACTA ACCTCGAACG GGAGGTTAGT AGGTCATGAT 
GGAGATATTG GGAATGCAGA TATGTGTTAT GGATATAATA GCAATGGAGG GTGCCAAAAA 
TGGGAGGAAA TACCAAATTG TAGGGAAAAT GGTGAGGTTT TTCAGAAAAT GGTGGGAACA 
CCAACCTTAG ACTATGAAAC AGTGTTTGAA TTCGATGTGA CTTATAGTTA CAGTGATTGC 
AAGATTAGAT GTTGGAGAAA TTGTTATTGC AATGGATTCC AGGAATTTTA TGGTAATGGG 
ACTGGATGTA CATTCTATTC ATGGAATTCA ACACAATATG TTGATTTGGT GAGTCAGAAT 
AACTTCTACG TCTTGGTAAA CTCTATAAAA TCAGCCCCAA ATTCCCATGT CAATGCAGGT 
AAAAAAAAGT GGATATGGAT AACTTCTACA ATCGCAGCTG CTTTACTCAT ATTTTGCCCA 
ATCATTTTAT GCTTAGCCAA AAAGAAACAA AAATATGCAC TTCAAGATAA GAAAAGTAAA 
AGAAAGGATT TGGCGGATTC GACTGAATCC TATAATATCA AAGATCTTGA ACATGACTTC 
AAGGAACATG ATATAAAAGT TTTTAACTTT ACATCAATTT TGGAAGCAAC TATGGACTTT 
TCACCTAAAA ACAAGTTAGG ACAGGGCGGC TATGGACCCA TTTATAAGGG AATCTTGGCA 
ACCGGGCAAG AAGTTGCTGT AAAAGGACTT TCAAAAACAT CCGGACAAGG AATTGTAGAA 
TTCAAAAATG AATTGGTCCT TATATGTGAA CTTCAGCACA GAAATCTTGT AGAACTACTT 
GGTTGTTGCA TTCATGAAGA AGAGAGGATT CTAATTTACG AATATATGTC AAACAAAAGC 
TTGGATTTCT ACCTATTTGA TTGTACAAAA AAGAAGCTGC TAGATTGGAA GAAGCGTTTT 
AACATAATAG AAGGAATTGC TCAAGGATTG CTCTATCTTC ATAAGTACTC AAGACTCAAA 
ATTATTCATA GAGACTTGAA GGCGAGCAAC ATACTTCTTG ATGAGAATAT GAACCCAAAA 
ATTTCTGATT TTGGTATGGC AAGAATGTTC ACACAACAGG AATCCGTAGT AAATACCAAC 
AGGATTGTTG GGACATATGG ATACATGTCT CCAGAATATG CCATGGAAGG AGTTTGTTCT 
ACAAAGTCTG ACGTCTATAG CTTTGGAGTG TTGCTACTTG AAATCGTTTG TGGGAGAAAA 
AACAATAGCT TCTATGACGT TGATCGCCCA CTAAATCTAA TAGGACATGC ATGGGAGCTA 
TGGAATGATG GTGAGTATCT ACAGTTAATG GATCCAACAT TAAATGACAC ATTTGTTCCT 
GATGAAGTGA AAAGGTGCAT TCATGTTGGT CTCTTATGTG TAGAGCAATA TGCAAATGAT 
CGACCAACAA TGTCTGATGT TATATCTGTG TTAACAAACA AATACCAACT CACAAACTTA 
CCAAGAAGAC CAGCATTTTA TGTTAGAAGA GAGATCTTTG AGGGGGAAAC AATTTCCAAA 
GGGCAAGACA CCGACACCTA TTCTACGACA GCAATTTCTA CTTCATGTGA AGAAGGGAAA 
ATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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MTR_8g052060 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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