LOC101484875 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101484875
Entrez Gene ID	101484875
Full Name	nuclear factor of activated T-cells 5
Gene Type	protein-coding
Organism	Maylandia zebra(zebra mbuna)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004553352.1	XP_004553409.1	nuclear factor of activated T-cells 5 isoform X1
XM_012919595.1	XP_012775049.1	nuclear factor of activated T-cells 5 isoform X2
XM_004553350.3	XP_004553407.1	nuclear factor of activated T-cells 5 isoform X1
XM_004553350.2	XP_004553407.1	nuclear factor of activated T-cells 5 isoform X1
XM_004553351.3	XP_004553408.1	nuclear factor of activated T-cells 5 isoform X1
XM_004553352.2	XP_004553409.1	nuclear factor of activated T-cells 5 isoform X1
XM_012919595.2	XP_012775049.1	nuclear factor of activated T-cells 5 isoform X2
XM_004553351.4	XP_004553408.1	nuclear factor of activated T-cells 5 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC101484875 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101484875 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMa375202	XM_004553350.3 Latest version!	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa375202	XM_004553352.2 Latest version!	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa375202	XM_004553351.4 Latest version!	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OMa375203	XM_012919595.2 Latest version!	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMa33419	XM_004553351.3 Latest version!	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OMa33419	XM_004553352.1 Latest version!	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OMa33419	XM_004553350.2 Latest version!	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OMa33420	XM_012919595.1 Latest version!	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101484875 ( XM_004553350.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004553407.1 Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa375202
Clone ID Related Accession (Same CDS sequence)	XM_004553350.3 , XM_004553352.2 , XM_004553351.4
Accession Version	XM_004553350.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004553350.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ATTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004553407.1
CDS	281..4537
Translation	MPSDFISLLSSDIDLNSPKSLYSKESVYDLLPKELQLQPSSTQTDPPTMSQNSGGEAGPPPSAALASDATSSTSSPSASSSLAIGVTSSGPSTSSSDHLKVAQHLHSSGGDGAGVSEMQGAEGAPNRGSSGANATVGDVGPGGLPGLGVQQPQNTPSKRRTVLSISPPPEDLFDDSQMSCQEEPTISGAPGPDSEQSSSMWADESVSNFSLISSISYNDNTEVPRKSRKRTPRQRPGPKPAPPEDSMDVFDADSAKAPHYVLSQLGTDKTSSISSSLESGTAVKGGSLSAQFPQRSDGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVSELVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDIEGTIVIEIPLEPSNDMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVSIPQPDGSTLTLQVPSSPILCTQPAGVPEILRKSLHSCSVKGGEEVFIIGKNFLKGTKVIFQENITDDNSWQAEAKIDMDLFHQNHLIVTVPPFHNQSITSPVSVGIFVMTNAGRSHEAQPFTYTPDSADNSNVLTVKTEGISMVKTCLFDTPIKSMPSEQTEISDQTSKRQEDTPMEVSSNPPSTEVFKSSPDPLIAVQQTLDLGSSSHPGGESFQRTMPLPSEDVDLPQAPPVFPSLESLSTIQKQEIAPTTSFSVSGDTTIPPVTPEVPQQFLRDPQESLSPESSSSSAGVVVVAMPQIAPSSQAQPQQSQVSLFPQEEVAQLERAVRELQGGSNTTLQQVLEAAVAQQQLNSVLYSPTPSAESLQQHVQENMNSLRLGSTDNSLSAQQQLQIQQQEQQQQIQQQFQQQQQILGNLQQQQQQVINNIQMQQQLILQPQEQQQIMENIQQQQLQQNQQQQVLNNIQLQDQQQQNHILTNLQQHQLQQQQQQQNQALSNLQQQQQQLQEQQVLENLQQHLQASSLLQQAGELLTIQTSFPTQPPSHTSPPQQLFQSPRPMAETQGSQQQVQAALIQNTLTVLTSGSLNSEQQSTGSTLYLTPNHQPQQQQQLAFISSMETSASQHQSVSMFQNQPQSQMQQQSTPMDQQQSPQQNQQQPAQLPMGQQGSLFQSIPNHSQGNPAPQQTGLLLCTTDLNPQAIAPTILFSTQTQGPSPMGTISVGITQSDTAEPMSFQDQSSSGSNSTTTENQQQSLFQEQQPMQVGPSSSSVQNNQPVELFIPQTNLSSLQSTIASQELNNQAPAPATTIFVVQGGVGVVASPGQQPPEQLFQTSVGGNVASQGQASLFVFGIQNDSSQLLTSSGPSQPAQNQIPNSGHMQTLLDQPMAQAASPIPATMHGSLQNTLQAQMQTTLENAMQANTQTALQSSLQGNIEASLPTPMQTSLQTQIQSSLQNQLQASISTSSSMDKIEDLLESLQKQ

Target ORF information:

RefSeq Version	XM_004553350.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004553350.3

ORF Insert Sequence:

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ATTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101484875 ( XM_004553352.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004553409.1 Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMa375202
Clone ID Related Accession (Same CDS sequence)	XM_004553350.3 , XM_004553352.2 , XM_004553351.4
Accession Version	XM_004553352.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004553352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ATTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004553409.1
CDS	80..4336
Translation	MPSDFISLLSSDIDLNSPKSLYSKESVYDLLPKELQLQPSSTQTDPPTMSQNSGGEAGPPPSAALASDATSSTSSPSASSSLAIGVTSSGPSTSSSDHLKVAQHLHSSGGDGAGVSEMQGAEGAPNRGSSGANATVGDVGPGGLPGLGVQQPQNTPSKRRTVLSISPPPEDLFDDSQMSCQEEPTISGAPGPDSEQSSSMWADESVSNFSLISSISYNDNTEVPRKSRKRTPRQRPGPKPAPPEDSMDVFDADSAKAPHYVLSQLGTDKTSSISSSLESGTAVKGGSLSAQFPQRSDGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVSELVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDIEGTIVIEIPLEPSNDMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVSIPQPDGSTLTLQVPSSPILCTQPAGVPEILRKSLHSCSVKGGEEVFIIGKNFLKGTKVIFQENITDDNSWQAEAKIDMDLFHQNHLIVTVPPFHNQSITSPVSVGIFVMTNAGRSHEAQPFTYTPDSADNSNVLTVKTEGISMVKTCLFDTPIKSMPSEQTEISDQTSKRQEDTPMEVSSNPPSTEVFKSSPDPLIAVQQTLDLGSSSHPGGESFQRTMPLPSEDVDLPQAPPVFPSLESLSTIQKQEIAPTTSFSVSGDTTIPPVTPEVPQQFLRDPQESLSPESSSSSAGVVVVAMPQIAPSSQAQPQQSQVSLFPQEEVAQLERAVRELQGGSNTTLQQVLEAAVAQQQLNSVLYSPTPSAESLQQHVQENMNSLRLGSTDNSLSAQQQLQIQQQEQQQQIQQQFQQQQQILGNLQQQQQQVINNIQMQQQLILQPQEQQQIMENIQQQQLQQNQQQQVLNNIQLQDQQQQNHILTNLQQHQLQQQQQQQNQALSNLQQQQQQLQEQQVLENLQQHLQASSLLQQAGELLTIQTSFPTQPPSHTSPPQQLFQSPRPMAETQGSQQQVQAALIQNTLTVLTSGSLNSEQQSTGSTLYLTPNHQPQQQQQLAFISSMETSASQHQSVSMFQNQPQSQMQQQSTPMDQQQSPQQNQQQPAQLPMGQQGSLFQSIPNHSQGNPAPQQTGLLLCTTDLNPQAIAPTILFSTQTQGPSPMGTISVGITQSDTAEPMSFQDQSSSGSNSTTTENQQQSLFQEQQPMQVGPSSSSVQNNQPVELFIPQTNLSSLQSTIASQELNNQAPAPATTIFVVQGGVGVVASPGQQPPEQLFQTSVGGNVASQGQASLFVFGIQNDSSQLLTSSGPSQPAQNQIPNSGHMQTLLDQPMAQAASPIPATMHGSLQNTLQAQMQTTLENAMQANTQTALQSSLQGNIEASLPTPMQTSLQTQIQSSLQNQLQASISTSSSMDKIEDLLESLQKQ

Target ORF information:

RefSeq Version	XM_004553352.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004553352.2

ORF Insert Sequence:

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ATTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101484875 ( XM_004553351.4 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004553408.1 Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X2, mRNA.

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CloneID	OMa375202
Clone ID Related Accession (Same CDS sequence)	XM_004553350.3 , XM_004553352.2 , XM_004553351.4
Accession Version	XM_004553351.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_004553351.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ATTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004553408.1
CDS	150..4406
Translation	MPSDFISLLSSDIDLNSPKSLYSKESVYDLLPKELQLQPSSTQTDPPTMSQNSGGEAGPPPSAALASDATSSTSSPSASSSLAIGVTSSGPSTSSSDHLKVAQHLHSSGGDGAGVSEMQGAEGAPNRGSSGANATVGDVGPGGLPGLGVQQPQNTPSKRRTVLSISPPPEDLFDDSQMSCQEEPTISGAPGPDSEQSSSMWADESVSNFSLISSISYNDNTEVPRKSRKRTPRQRPGPKPAPPEDSMDVFDADSAKAPHYVLSQLGTDKTSSISSSLESGTAVKGGSLSAQFPQRSDGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVSELVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDIEGTIVIEIPLEPSNDMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVSIPQPDGSTLTLQVPSSPILCTQPAGVPEILRKSLHSCSVKGGEEVFIIGKNFLKGTKVIFQENITDDNSWQAEAKIDMDLFHQNHLIVTVPPFHNQSITSPVSVGIFVMTNAGRSHEAQPFTYTPDSADNSNVLTVKTEGISMVKTCLFDTPIKSMPSEQTEISDQTSKRQEDTPMEVSSNPPSTEVFKSSPDPLIAVQQTLDLGSSSHPGGESFQRTMPLPSEDVDLPQAPPVFPSLESLSTIQKQEIAPTTSFSVSGDTTIPPVTPEVPQQFLRDPQESLSPESSSSSAGVVVVAMPQIAPSSQAQPQQSQVSLFPQEEVAQLERAVRELQGGSNTTLQQVLEAAVAQQQLNSVLYSPTPSAESLQQHVQENMNSLRLGSTDNSLSAQQQLQIQQQEQQQQIQQQFQQQQQILGNLQQQQQQVINNIQMQQQLILQPQEQQQIMENIQQQQLQQNQQQQVLNNIQLQDQQQQNHILTNLQQHQLQQQQQQQNQALSNLQQQQQQLQEQQVLENLQQHLQASSLLQQAGELLTIQTSFPTQPPSHTSPPQQLFQSPRPMAETQGSQQQVQAALIQNTLTVLTSGSLNSEQQSTGSTLYLTPNHQPQQQQQLAFISSMETSASQHQSVSMFQNQPQSQMQQQSTPMDQQQSPQQNQQQPAQLPMGQQGSLFQSIPNHSQGNPAPQQTGLLLCTTDLNPQAIAPTILFSTQTQGPSPMGTISVGITQSDTAEPMSFQDQSSSGSNSTTTENQQQSLFQEQQPMQVGPSSSSVQNNQPVELFIPQTNLSSLQSTIASQELNNQAPAPATTIFVVQGGVGVVASPGQQPPEQLFQTSVGGNVASQGQASLFVFGIQNDSSQLLTSSGPSQPAQNQIPNSGHMQTLLDQPMAQAASPIPATMHGSLQNTLQAQMQTTLENAMQANTQTALQSSLQGNIEASLPTPMQTSLQTQIQSSLQNQLQASISTSSSMDKIEDLLESLQKQ

Target ORF information:

RefSeq Version	XM_004553351.4
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004553351.4

ORF Insert Sequence:

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ATTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101484875 ( XM_012919595.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012775049.1 Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X4, mRNA.

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CloneID	OMa375203
Clone ID Related Accession (Same CDS sequence)	XM_012919595.2
Accession Version	XM_012919595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-23
Organism	Maylandia zebra(zebra mbuna)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036786.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 24, 2018 this sequence version replaced XM_012919595.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGTG CGGAGGGTGC CCCCAACAGA GGCAGCAGTG GAGCAAACGC TACAGTAGGG 
GATGTAGGGC CGGGAGGGTT GCCGGGGCTA GGAGTCCAGC AGCCCCAGAA CACTCCATCA 
AAGCGAAGGA CTGTGTTGAG CATATCCCCA CCACCCGAGG ATCTGTTTGA TGACAGCCAG 
ATGTCTTGCC AAGAGGAACC TACCATATCT GGTGCACCAG GTCCCGACTC AGAGCAAAGC 
AGCAGCATGT GGGCCGATGA GTCTGTCTCG AACTTCAGTT TGATCAGCTC AATCTCCTAC 
AATGACAACA CGGAGGTGCC ACGCAAGTCT AGAAAACGCA CTCCTCGCCA GCGGCCCGGT 
CCCAAGCCTG CTCCTCCAGA AGACAGCATG GATGTGTTTG ATGCTGACAG CGCCAAAGCC 
CCTCACTATG TGCTATCCCA GCTGGGCACA GATAAGACCA GTTCCATATC CAGCTCTCTC 
GAGTCAGGAA CTGCAGTGAA AGGTGGGTCA CTGTCTGCTC AGTTCCCTCA GAGGAGTGAT 
GGGAAGGAGC TAAAGATCCT AGTGCAGCCA GAGACCCAGC ACAGAGCTCG CTATCTGACT 
GAGGGCAGCA GAGGTTCTGT CAAAGATCGT ACACAACAAG GATTCCCCAC TGTCAAGTTG 
GAAGGTGTGA GTGAATTAGT TGTGCTGCAG GTGTTTGTAG CAAATGATGC AGGCAGAGTG 
AAGCCTCACG GTTTCTACCA GGCATGCAGA GTGACAGGAC GCAACACCAC TGCTTGCAAA 
GAGGTGGACA TAGAAGGCAC CATAGTTATT GAGATTCCTC TGGAGCCCAG CAATGACATG 
ACACTTGCGG TGGACTGTGT AGGAATTTTG AAGCTCCGTA ATGCAGATGT CGAAGCCCGC 
ATTGGTGTTG CAGGATCCAA GAAGAAGAGT ACACGAGCCA GGTTGGCCTT CAGAGTCAGC 
ATCCCCCAGC CTGATGGATC AACCCTGACT TTACAGGTTC CCTCATCGCC GATCCTCTGC 
ACTCAGCCAG CAGGAGTGCC AGAGATCCTG AGAAAGAGTC TCCACAGCTG CTCAGTGAAA 
GGAGGCGAGG AAGTTTTTAT AATCGGAAAG AACTTCCTTA AAGGAACCAA AGTTATTTTT 
CAGGAGAACA TTACAGATGA TAATTCCTGG CAAGCCGAAG CAAAGATTGA CATGGATCTT 
TTCCATCAGA ATCACTTGAT AGTGACAGTC CCTCCGTTTC ACAACCAGTC AATCACTTCT 
CCTGTTTCTG TGGGTATTTT TGTGATGACC AATGCCGGTA GATCACATGA AGCCCAACCC 
TTCACCTACA CTCCAGACTC GGCTGATAAC TCAAATGTCC TGACAGTAAA AACGGAGGGG 
ATATCCATGG TCAAGACCTG CTTATTTGAT ACCCCGATCA AATCTATGCC CTCTGAACAA 
ACAGAAATCT CTGACCAGAC ATCCAAACGG CAAGAGGACA CACCTATGGA AGTGTCTAGC 
AATCCACCAT CCACAGAGGT CTTCAAATCA TCCCCTGATC CACTCATCGC AGTGCAGCAG 
ACTCTGGACC TTGGCTCTAG TTCTCATCCA GGTGGAGAGT CCTTTCAGAG AACAATGCCT 
TTACCATCAG AAGATGTGGA CCTTCCCCAG GCACCCCCGG TCTTCCCTAG CTTAGAGTCC 
CTCAGCACGA TACAAAAGCA AGAAATTGCC CCAACAACCT CCTTCTCTGT GTCAGGAGAC 
ACCACAATAC CACCTGTGAC ACCAGAAGTC CCCCAGCAGT TCCTTAGAGA CCCCCAGGAA 
AGCTTGTCAC CTGAGAGTTC CAGTAGTAGT GCAGGGGTGG TGGTTGTGGC CATGCCACAG 
ATAGCACCTT CCTCTCAGGC ACAGCCACAG CAGTCCCAGG TCTCCCTCTT TCCCCAGGAA 
GAGGTGGCCC AGCTGGAAAG GGCAGTTAGA GAACTCCAGG GGGGAAGTAA CACCACGCTC 
CAGCAGGTGT TAGAAGCAGC TGTAGCTCAG CAGCAGCTAA ATTCTGTGCT GTATAGCCCC 
ACACCTTCTG CAGAATCCCT TCAGCAGCAT GTCCAGGAGA ACATGAACAG CCTTAGACTG 
GGGAGTACAG ATAATTCACT GTCAGCACAG CAACAGCTAC AGATACAACA ACAGGAGCAG 
CAACAACAAA TACAACAGCA GTTTCAACAA CAGCAACAAA TACTTGGAAA CCTTCAGCAG 
CAACAGCAGC AAGTCATCAA CAACATACAG ATGCAACAGC AACTTATATT ACAGCCACAA 
GAGCAACAGC AAATTATGGA GAATATTCAA CAACAGCAAT TGCAACAAAA TCAGCAACAG 
CAAGTTCTAA ACAATATTCA GCTTCAGGAT CAGCAACAAC AAAATCATAT TCTTACCAAT 
TTACAGCAAC ATCAGCTGCA ACAACAGCAG CAGCAGCAAA ATCAAGCGTT GAGCAACCTA 
CAACAGCAAC AGCAGCAACT TCAAGAGCAG CAGGTTTTGG AAAATTTACA GCAACACCTT 
CAGGCCAGCT CTCTCCTTCA ACAGGCTGGA GAACTGCTTA CTATCCAGAC TAGCTTCCCA 
ACACAACCTC CTTCACACAC ATCCCCCCCA CAGCAACTCT TCCAGTCGCC TAGGCCCATG 
GCCGAAACCC AGGGGTCCCA ACAGCAGGTC CAGGCTGCCC TCATCCAGAA TACCCTGACC 
GTACTGACTA GTGGCAGTCT CAACTCAGAG CAGCAGTCCA CAGGATCAAC CCTATACCTG 
ACGCCGAACC ATCAGCCCCA GCAACAGCAG CAGCTGGCAT TCATCTCCTC CATGGAGACA 
TCAGCCAGCC AACATCAGTC TGTTTCAATG TTTCAAAATC AACCGCAGTC TCAAATGCAG 
CAACAGAGCA CCCCAATGGA TCAGCAGCAG TCCCCACAGC AGAACCAACA ACAACCAGCA 
CAGCTTCCAA TGGGCCAGCA GGGTTCCTTA TTTCAAAGTA TTCCCAATCA TTCACAGGGC 
AACCCTGCAC CACAGCAAAC AGGTCTGCTT CTCTGCACCA CAGATCTGAA CCCACAGGCT 
ATCGCCCCTA CTATTCTCTT TAGCACCCAG ACCCAAGGCC CTTCCCCTAT GGGGACCATT 
AGCGTTGGAA TCACCCAGTC AGATACAGCA GAGCCCATGT CCTTCCAAGA CCAGAGCTCT 
TCGGGCAGCA ACTCAACAAC CACTGAGAAC CAACAACAGA GCTTGTTCCA GGAACAGCAG 
CCAATGCAAG TGGGCCCAAG TTCAAGCTCC GTCCAAAACA ATCAACCTGT GGAGCTGTTC 
ATACCACAGA CAAATCTGTC CAGCTTGCAG AGTACTATTG CCTCACAGGA GCTCAACAAC 
CAGGCTCCAG CCCCCGCCAC AACCATCTTT GTGGTGCAGG GTGGTGTGGG TGTGGTAGCC 
AGCCCTGGTC AGCAGCCCCC AGAGCAGCTT TTCCAGACAT CTGTGGGTGG CAATGTGGCT 
TCTCAAGGAC AGGCCAGTCT GTTTGTTTTT GGCATTCAGA ATGACTCATC CCAGCTGCTC 
ACTTCTTCTG GACCCAGCCA ACCTGCGCAG AACCAAATCC CGAACTCCGG TCACATGCAG 
ACTCTGCTGG ATCAGCCCAT GGCCCAGGCT GCCTCCCCAA TCCCAGCTAC CATGCATGGC 
AGCTTGCAGA ACACCCTTCA GGCACAAATG CAGACCACCT TGGAGAACGC AATGCAGGCA 
AACACACAAA CAGCCCTTCA GTCCAGTTTA CAAGGAAACA TAGAAGCGAG TCTACCAACA 
CCAATGCAGA CCAGTCTACA GACACAGATA CAGAGCAGCT TACAGAATCA ACTGCAGGCA 
TCCATATCAA CATCGTCCAG CATGGATAAA ATTGAGGACC TACTGGAAAG CCTACAGAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012775049.1
CDS	302..4207
Translation	MQGAEGAPNRGSSGANATVGDVGPGGLPGLGVQQPQNTPSKRRTVLSISPPPEDLFDDSQMSCQEEPTISGAPGPDSEQSSSMWADESVSNFSLISSISYNDNTEVPRKSRKRTPRQRPGPKPAPPEDSMDVFDADSAKAPHYVLSQLGTDKTSSISSSLESGTAVKGGSLSAQFPQRSDGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVSELVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDIEGTIVIEIPLEPSNDMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVSIPQPDGSTLTLQVPSSPILCTQPAGVPEILRKSLHSCSVKGGEEVFIIGKNFLKGTKVIFQENITDDNSWQAEAKIDMDLFHQNHLIVTVPPFHNQSITSPVSVGIFVMTNAGRSHEAQPFTYTPDSADNSNVLTVKTEGISMVKTCLFDTPIKSMPSEQTEISDQTSKRQEDTPMEVSSNPPSTEVFKSSPDPLIAVQQTLDLGSSSHPGGESFQRTMPLPSEDVDLPQAPPVFPSLESLSTIQKQEIAPTTSFSVSGDTTIPPVTPEVPQQFLRDPQESLSPESSSSSAGVVVVAMPQIAPSSQAQPQQSQVSLFPQEEVAQLERAVRELQGGSNTTLQQVLEAAVAQQQLNSVLYSPTPSAESLQQHVQENMNSLRLGSTDNSLSAQQQLQIQQQEQQQQIQQQFQQQQQILGNLQQQQQQVINNIQMQQQLILQPQEQQQIMENIQQQQLQQNQQQQVLNNIQLQDQQQQNHILTNLQQHQLQQQQQQQNQALSNLQQQQQQLQEQQVLENLQQHLQASSLLQQAGELLTIQTSFPTQPPSHTSPPQQLFQSPRPMAETQGSQQQVQAALIQNTLTVLTSGSLNSEQQSTGSTLYLTPNHQPQQQQQLAFISSMETSASQHQSVSMFQNQPQSQMQQQSTPMDQQQSPQQNQQQPAQLPMGQQGSLFQSIPNHSQGNPAPQQTGLLLCTTDLNPQAIAPTILFSTQTQGPSPMGTISVGITQSDTAEPMSFQDQSSSGSNSTTTENQQQSLFQEQQPMQVGPSSSSVQNNQPVELFIPQTNLSSLQSTIASQELNNQAPAPATTIFVVQGGVGVVASPGQQPPEQLFQTSVGGNVASQGQASLFVFGIQNDSSQLLTSSGPSQPAQNQIPNSGHMQTLLDQPMAQAASPIPATMHGSLQNTLQAQMQTTLENAMQANTQTALQSSLQGNIEASLPTPMQTSLQTQIQSSLQNQLQASISTSSSMDKIEDLLESLQKQ

Target ORF information:

RefSeq Version	XM_012919595.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012919595.2

ORF Insert Sequence:

ATGCAAGGTG CGGAGGGTGC CCCCAACAGA GGCAGCAGTG GAGCAAACGC TACAGTAGGG 
GATGTAGGGC CGGGAGGGTT GCCGGGGCTA GGAGTCCAGC AGCCCCAGAA CACTCCATCA 
AAGCGAAGGA CTGTGTTGAG CATATCCCCA CCACCCGAGG ATCTGTTTGA TGACAGCCAG 
ATGTCTTGCC AAGAGGAACC TACCATATCT GGTGCACCAG GTCCCGACTC AGAGCAAAGC 
AGCAGCATGT GGGCCGATGA GTCTGTCTCG AACTTCAGTT TGATCAGCTC AATCTCCTAC 
AATGACAACA CGGAGGTGCC ACGCAAGTCT AGAAAACGCA CTCCTCGCCA GCGGCCCGGT 
CCCAAGCCTG CTCCTCCAGA AGACAGCATG GATGTGTTTG ATGCTGACAG CGCCAAAGCC 
CCTCACTATG TGCTATCCCA GCTGGGCACA GATAAGACCA GTTCCATATC CAGCTCTCTC 
GAGTCAGGAA CTGCAGTGAA AGGTGGGTCA CTGTCTGCTC AGTTCCCTCA GAGGAGTGAT 
GGGAAGGAGC TAAAGATCCT AGTGCAGCCA GAGACCCAGC ACAGAGCTCG CTATCTGACT 
GAGGGCAGCA GAGGTTCTGT CAAAGATCGT ACACAACAAG GATTCCCCAC TGTCAAGTTG 
GAAGGTGTGA GTGAATTAGT TGTGCTGCAG GTGTTTGTAG CAAATGATGC AGGCAGAGTG 
AAGCCTCACG GTTTCTACCA GGCATGCAGA GTGACAGGAC GCAACACCAC TGCTTGCAAA 
GAGGTGGACA TAGAAGGCAC CATAGTTATT GAGATTCCTC TGGAGCCCAG CAATGACATG 
ACACTTGCGG TGGACTGTGT AGGAATTTTG AAGCTCCGTA ATGCAGATGT CGAAGCCCGC 
ATTGGTGTTG CAGGATCCAA GAAGAAGAGT ACACGAGCCA GGTTGGCCTT CAGAGTCAGC 
ATCCCCCAGC CTGATGGATC AACCCTGACT TTACAGGTTC CCTCATCGCC GATCCTCTGC 
ACTCAGCCAG CAGGAGTGCC AGAGATCCTG AGAAAGAGTC TCCACAGCTG CTCAGTGAAA 
GGAGGCGAGG AAGTTTTTAT AATCGGAAAG AACTTCCTTA AAGGAACCAA AGTTATTTTT 
CAGGAGAACA TTACAGATGA TAATTCCTGG CAAGCCGAAG CAAAGATTGA CATGGATCTT 
TTCCATCAGA ATCACTTGAT AGTGACAGTC CCTCCGTTTC ACAACCAGTC AATCACTTCT 
CCTGTTTCTG TGGGTATTTT TGTGATGACC AATGCCGGTA GATCACATGA AGCCCAACCC 
TTCACCTACA CTCCAGACTC GGCTGATAAC TCAAATGTCC TGACAGTAAA AACGGAGGGG 
ATATCCATGG TCAAGACCTG CTTATTTGAT ACCCCGATCA AATCTATGCC CTCTGAACAA 
ACAGAAATCT CTGACCAGAC ATCCAAACGG CAAGAGGACA CACCTATGGA AGTGTCTAGC 
AATCCACCAT CCACAGAGGT CTTCAAATCA TCCCCTGATC CACTCATCGC AGTGCAGCAG 
ACTCTGGACC TTGGCTCTAG TTCTCATCCA GGTGGAGAGT CCTTTCAGAG AACAATGCCT 
TTACCATCAG AAGATGTGGA CCTTCCCCAG GCACCCCCGG TCTTCCCTAG CTTAGAGTCC 
CTCAGCACGA TACAAAAGCA AGAAATTGCC CCAACAACCT CCTTCTCTGT GTCAGGAGAC 
ACCACAATAC CACCTGTGAC ACCAGAAGTC CCCCAGCAGT TCCTTAGAGA CCCCCAGGAA 
AGCTTGTCAC CTGAGAGTTC CAGTAGTAGT GCAGGGGTGG TGGTTGTGGC CATGCCACAG 
ATAGCACCTT CCTCTCAGGC ACAGCCACAG CAGTCCCAGG TCTCCCTCTT TCCCCAGGAA 
GAGGTGGCCC AGCTGGAAAG GGCAGTTAGA GAACTCCAGG GGGGAAGTAA CACCACGCTC 
CAGCAGGTGT TAGAAGCAGC TGTAGCTCAG CAGCAGCTAA ATTCTGTGCT GTATAGCCCC 
ACACCTTCTG CAGAATCCCT TCAGCAGCAT GTCCAGGAGA ACATGAACAG CCTTAGACTG 
GGGAGTACAG ATAATTCACT GTCAGCACAG CAACAGCTAC AGATACAACA ACAGGAGCAG 
CAACAACAAA TACAACAGCA GTTTCAACAA CAGCAACAAA TACTTGGAAA CCTTCAGCAG 
CAACAGCAGC AAGTCATCAA CAACATACAG ATGCAACAGC AACTTATATT ACAGCCACAA 
GAGCAACAGC AAATTATGGA GAATATTCAA CAACAGCAAT TGCAACAAAA TCAGCAACAG 
CAAGTTCTAA ACAATATTCA GCTTCAGGAT CAGCAACAAC AAAATCATAT TCTTACCAAT 
TTACAGCAAC ATCAGCTGCA ACAACAGCAG CAGCAGCAAA ATCAAGCGTT GAGCAACCTA 
CAACAGCAAC AGCAGCAACT TCAAGAGCAG CAGGTTTTGG AAAATTTACA GCAACACCTT 
CAGGCCAGCT CTCTCCTTCA ACAGGCTGGA GAACTGCTTA CTATCCAGAC TAGCTTCCCA 
ACACAACCTC CTTCACACAC ATCCCCCCCA CAGCAACTCT TCCAGTCGCC TAGGCCCATG 
GCCGAAACCC AGGGGTCCCA ACAGCAGGTC CAGGCTGCCC TCATCCAGAA TACCCTGACC 
GTACTGACTA GTGGCAGTCT CAACTCAGAG CAGCAGTCCA CAGGATCAAC CCTATACCTG 
ACGCCGAACC ATCAGCCCCA GCAACAGCAG CAGCTGGCAT TCATCTCCTC CATGGAGACA 
TCAGCCAGCC AACATCAGTC TGTTTCAATG TTTCAAAATC AACCGCAGTC TCAAATGCAG 
CAACAGAGCA CCCCAATGGA TCAGCAGCAG TCCCCACAGC AGAACCAACA ACAACCAGCA 
CAGCTTCCAA TGGGCCAGCA GGGTTCCTTA TTTCAAAGTA TTCCCAATCA TTCACAGGGC 
AACCCTGCAC CACAGCAAAC AGGTCTGCTT CTCTGCACCA CAGATCTGAA CCCACAGGCT 
ATCGCCCCTA CTATTCTCTT TAGCACCCAG ACCCAAGGCC CTTCCCCTAT GGGGACCATT 
AGCGTTGGAA TCACCCAGTC AGATACAGCA GAGCCCATGT CCTTCCAAGA CCAGAGCTCT 
TCGGGCAGCA ACTCAACAAC CACTGAGAAC CAACAACAGA GCTTGTTCCA GGAACAGCAG 
CCAATGCAAG TGGGCCCAAG TTCAAGCTCC GTCCAAAACA ATCAACCTGT GGAGCTGTTC 
ATACCACAGA CAAATCTGTC CAGCTTGCAG AGTACTATTG CCTCACAGGA GCTCAACAAC 
CAGGCTCCAG CCCCCGCCAC AACCATCTTT GTGGTGCAGG GTGGTGTGGG TGTGGTAGCC 
AGCCCTGGTC AGCAGCCCCC AGAGCAGCTT TTCCAGACAT CTGTGGGTGG CAATGTGGCT 
TCTCAAGGAC AGGCCAGTCT GTTTGTTTTT GGCATTCAGA ATGACTCATC CCAGCTGCTC 
ACTTCTTCTG GACCCAGCCA ACCTGCGCAG AACCAAATCC CGAACTCCGG TCACATGCAG 
ACTCTGCTGG ATCAGCCCAT GGCCCAGGCT GCCTCCCCAA TCCCAGCTAC CATGCATGGC 
AGCTTGCAGA ACACCCTTCA GGCACAAATG CAGACCACCT TGGAGAACGC AATGCAGGCA 
AACACACAAA CAGCCCTTCA GTCCAGTTTA CAAGGAAACA TAGAAGCGAG TCTACCAACA 
CCAATGCAGA CCAGTCTACA GACACAGATA CAGAGCAGCT TACAGAATCA ACTGCAGGCA 
TCCATATCAA CATCGTCCAG CATGGATAAA ATTGAGGACC TACTGGAAAG CCTACAGAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101484875 ( XM_004553352.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004553409.1 Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X3, mRNA.

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CloneID	OMa33419
Clone ID Related Accession (Same CDS sequence)	XM_004553352.1 , XM_004553350.2 , XM_004553351.3
Accession Version	XM_004553352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447717.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ACTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004553409.1
CDS	80..4336
Translation	MPSDFISLLSSDIDLNSPKSLYSKESVYDLLPKELQLQPSSTQTDPPTMSQNSGGEAGPPPSAALASDATSSTSSPSASSSLAIGVTSSGPSTSSSDHLKVAQHLHSSGGDGAGVSEMQGAEGAPNRGSSGANATVGDVGPGGLPGLGVQQPQNTPSKRRTVLSISPPPEDLFDDSQMSCQEEPTISGAPGPDSEQSSSMWADESVSNFSLISSISYNDNTEVPRKSRKRTPRQRPGPKPAPPEDSMDVFDADSAKAPHYVLSQLGTDKTSSISSSLESGTAVKGGSLSAQFPQRSDGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVSELVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDIEGTIVIEIPLEPSNDMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVSIPQPDGSTLTLQVPSSPILCTQPAGVPEILRKSLHSCSVKGGEEVFIIGKNFLKGTKVIFQENITDDNSWQAEAKIDMDLFHQNHLIVTVPPFHNQSITSPVSVGIFVMTNAGRSHEAQPFTYTPDSADNSNVLTVKTEGISMVKTCLFDTPIKSMPSEQTEISDQTSKRQEDTPMEVSSNPPSTEVFKSSPDPLIAVQQTLDLGSSSHPGGESFQRTMPLPSEDVDLPQAPPVFPSLESLSTIQKQEIAPTTSFSVSGDTTIPPVTPEVPQQFLRDPQESLSPESSSSSAGVVVVAMPQIAPSSQAQPQQSQVSLFPQEEVAQLERAVRELQGGSNTTLQQVLEAAVAQQQLNSVLYSPTPSAESLQQHVQENMNSLRLGSTDNSLSAQQQLQIQQQEQQQQIQQQFQQQQQILGNLQQQQQQVINNIQMQQQLILQPQEQQQIMENIQQQQLQQNQQQQVLNNIQLQDQQQQNHILTNLQQHQLQQQQQQQNQALSNLQQQQQQLQEQQVLENLQQHLQASSLLQQAGELLTIQTSFPTQPPSHTSPPQQLFQSPRPMAETQGSQQQVQAALIQNTLTVLTSGSLNSEQQSTGSTLYLTPNHQPQQQQQLAFISSMETSASQHQSVSMFQNQPQSQMQQQSTPMDQQQSPQQNQQQPAQLPMGQQGSLFQSIPNHSQGNPAPQQTGLLLCTTDLNPQAIAPTILFSTQTQGPSPMGTISVGITQSDTAEPMSFQDQSSSGSNSTTTENQQQSLFQEQQPMQVGPSSSSVQNNQPVELFIPQTNLSSLQSTIASQELNNQAPAPATTIFVVQGGVGVVASPGQQPPEQLFQTSVGGNVASQGQASLFVFGIQNDSSQLLTSSGPSQPAQNQIPNSGHMQTLLDQPMAQAASPIPATMHGSLQNTLQAQMQTTLENAMQANTQTALQSSLQGNIEASLPTPMQTSLQTQIQSSLQNQLQASISTSSSMDKIEDLLESLQKQ

Target ORF information:

RefSeq Version	XM_004553352.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004553352.1

ORF Insert Sequence:

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ACTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101484875 ( XM_004553350.2 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004553407.1 Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMa33419
Clone ID Related Accession (Same CDS sequence)	XM_004553352.1 , XM_004553350.2 , XM_004553351.3
Accession Version	XM_004553350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447717.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 17, 2015 this sequence version replaced XM_004553350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ACTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004553407.1
CDS	281..4537
Translation	MPSDFISLLSSDIDLNSPKSLYSKESVYDLLPKELQLQPSSTQTDPPTMSQNSGGEAGPPPSAALASDATSSTSSPSASSSLAIGVTSSGPSTSSSDHLKVAQHLHSSGGDGAGVSEMQGAEGAPNRGSSGANATVGDVGPGGLPGLGVQQPQNTPSKRRTVLSISPPPEDLFDDSQMSCQEEPTISGAPGPDSEQSSSMWADESVSNFSLISSISYNDNTEVPRKSRKRTPRQRPGPKPAPPEDSMDVFDADSAKAPHYVLSQLGTDKTSSISSSLESGTAVKGGSLSAQFPQRSDGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVSELVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDIEGTIVIEIPLEPSNDMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVSIPQPDGSTLTLQVPSSPILCTQPAGVPEILRKSLHSCSVKGGEEVFIIGKNFLKGTKVIFQENITDDNSWQAEAKIDMDLFHQNHLIVTVPPFHNQSITSPVSVGIFVMTNAGRSHEAQPFTYTPDSADNSNVLTVKTEGISMVKTCLFDTPIKSMPSEQTEISDQTSKRQEDTPMEVSSNPPSTEVFKSSPDPLIAVQQTLDLGSSSHPGGESFQRTMPLPSEDVDLPQAPPVFPSLESLSTIQKQEIAPTTSFSVSGDTTIPPVTPEVPQQFLRDPQESLSPESSSSSAGVVVVAMPQIAPSSQAQPQQSQVSLFPQEEVAQLERAVRELQGGSNTTLQQVLEAAVAQQQLNSVLYSPTPSAESLQQHVQENMNSLRLGSTDNSLSAQQQLQIQQQEQQQQIQQQFQQQQQILGNLQQQQQQVINNIQMQQQLILQPQEQQQIMENIQQQQLQQNQQQQVLNNIQLQDQQQQNHILTNLQQHQLQQQQQQQNQALSNLQQQQQQLQEQQVLENLQQHLQASSLLQQAGELLTIQTSFPTQPPSHTSPPQQLFQSPRPMAETQGSQQQVQAALIQNTLTVLTSGSLNSEQQSTGSTLYLTPNHQPQQQQQLAFISSMETSASQHQSVSMFQNQPQSQMQQQSTPMDQQQSPQQNQQQPAQLPMGQQGSLFQSIPNHSQGNPAPQQTGLLLCTTDLNPQAIAPTILFSTQTQGPSPMGTISVGITQSDTAEPMSFQDQSSSGSNSTTTENQQQSLFQEQQPMQVGPSSSSVQNNQPVELFIPQTNLSSLQSTIASQELNNQAPAPATTIFVVQGGVGVVASPGQQPPEQLFQTSVGGNVASQGQASLFVFGIQNDSSQLLTSSGPSQPAQNQIPNSGHMQTLLDQPMAQAASPIPATMHGSLQNTLQAQMQTTLENAMQANTQTALQSSLQGNIEASLPTPMQTSLQTQIQSSLQNQLQASISTSSSMDKIEDLLESLQKQ

Target ORF information:

RefSeq Version	XM_004553350.2
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004553350.2

ORF Insert Sequence:

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ACTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101484875 ( XM_004553351.3 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_004553408.1 Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X2, mRNA.

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CloneID	OMa33419
Clone ID Related Accession (Same CDS sequence)	XM_004553352.1 , XM_004553350.2 , XM_004553351.3
Accession Version	XM_004553351.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	nuclear factor of activated T-cells 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447717.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 16, 2015 this sequence version replaced XM_004553351.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ACTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004553408.1
CDS	150..4406
Translation	MPSDFISLLSSDIDLNSPKSLYSKESVYDLLPKELQLQPSSTQTDPPTMSQNSGGEAGPPPSAALASDATSSTSSPSASSSLAIGVTSSGPSTSSSDHLKVAQHLHSSGGDGAGVSEMQGAEGAPNRGSSGANATVGDVGPGGLPGLGVQQPQNTPSKRRTVLSISPPPEDLFDDSQMSCQEEPTISGAPGPDSEQSSSMWADESVSNFSLISSISYNDNTEVPRKSRKRTPRQRPGPKPAPPEDSMDVFDADSAKAPHYVLSQLGTDKTSSISSSLESGTAVKGGSLSAQFPQRSDGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVSELVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDIEGTIVIEIPLEPSNDMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVSIPQPDGSTLTLQVPSSPILCTQPAGVPEILRKSLHSCSVKGGEEVFIIGKNFLKGTKVIFQENITDDNSWQAEAKIDMDLFHQNHLIVTVPPFHNQSITSPVSVGIFVMTNAGRSHEAQPFTYTPDSADNSNVLTVKTEGISMVKTCLFDTPIKSMPSEQTEISDQTSKRQEDTPMEVSSNPPSTEVFKSSPDPLIAVQQTLDLGSSSHPGGESFQRTMPLPSEDVDLPQAPPVFPSLESLSTIQKQEIAPTTSFSVSGDTTIPPVTPEVPQQFLRDPQESLSPESSSSSAGVVVVAMPQIAPSSQAQPQQSQVSLFPQEEVAQLERAVRELQGGSNTTLQQVLEAAVAQQQLNSVLYSPTPSAESLQQHVQENMNSLRLGSTDNSLSAQQQLQIQQQEQQQQIQQQFQQQQQILGNLQQQQQQVINNIQMQQQLILQPQEQQQIMENIQQQQLQQNQQQQVLNNIQLQDQQQQNHILTNLQQHQLQQQQQQQNQALSNLQQQQQQLQEQQVLENLQQHLQASSLLQQAGELLTIQTSFPTQPPSHTSPPQQLFQSPRPMAETQGSQQQVQAALIQNTLTVLTSGSLNSEQQSTGSTLYLTPNHQPQQQQQLAFISSMETSASQHQSVSMFQNQPQSQMQQQSTPMDQQQSPQQNQQQPAQLPMGQQGSLFQSIPNHSQGNPAPQQTGLLLCTTDLNPQAIAPTILFSTQTQGPSPMGTISVGITQSDTAEPMSFQDQSSSGSNSTTTENQQQSLFQEQQPMQVGPSSSSVQNNQPVELFIPQTNLSSLQSTIASQELNNQAPAPATTIFVVQGGVGVVASPGQQPPEQLFQTSVGGNVASQGQASLFVFGIQNDSSQLLTSSGPSQPAQNQIPNSGHMQTLLDQPMAQAASPIPATMHGSLQNTLQAQMQTTLENAMQANTQTALQSSLQGNIEASLPTPMQTSLQTQIQSSLQNQLQASISTSSSMDKIEDLLESLQKQ

Target ORF information:

RefSeq Version	XM_004553351.3
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004553351.3

ORF Insert Sequence:

ATGCCCTCTG ATTTTATATC CCTCCTTAGC TCGGATATCG ACCTCAATTC CCCCAAATCT 
CTATACTCTA AAGAGTCGGT ATATGACCTC CTCCCCAAAG AACTCCAGCT CCAGCCATCG 
TCCACCCAGA CAGACCCACC CACCATGAGC CAGAATAGTG GGGGAGAGGC GGGACCTCCC 
CCCTCTGCAG CCTTGGCGTC AGATGCCACC TCTTCCACCT CCAGCCCTTC TGCCTCTTCC 
TCCCTCGCCA TAGGCGTTAC ATCCTCTGGC CCCTCTACCT CATCGTCAGA CCATCTCAAG 
GTTGCCCAGC ATCTCCACTC CAGCGGAGGA GATGGCGCTG GTGTTTCAGA GATGCAAGGT 
GCGGAGGGTG CCCCCAACAG AGGCAGCAGT GGAGCAAACG CTACAGTAGG GGATGTAGGG 
CCGGGAGGGT TGCCGGGGCT AGGAGTCCAG CAGCCCCAGA ACACTCCATC AAAGCGAAGG 
ACTGTGTTGA GCATATCCCC ACCACCCGAG GATCTGTTTG ATGACAGCCA GATGTCTTGC 
CAAGAGGAAC CTACCATATC TGGTGCACCA GGTCCCGACT CAGAGCAAAG CAGCAGCATG 
TGGGCCGATG AGTCTGTCTC GAACTTCAGT TTGATCAGCT CAATCTCCTA CAATGACAAC 
ACGGAGGTGC CACGCAAGTC TAGAAAACGC ACTCCTCGCC AGCGGCCCGG TCCCAAGCCT 
GCTCCTCCAG AAGACAGCAT GGATGTGTTT GATGCTGACA GCGCCAAAGC CCCTCACTAT 
GTGCTATCCC AGCTGGGCAC AGATAAGACC AGTTCCATAT CCAGCTCTCT CGAGTCAGGA 
ACTGCAGTGA AAGGTGGGTC ACTGTCTGCT CAGTTCCCTC AGAGGAGTGA TGGGAAGGAG 
CTAAAGATCC TAGTGCAGCC AGAGACCCAG CACAGAGCTC GCTATCTGAC TGAGGGCAGC 
AGAGGTTCTG TCAAAGATCG TACACAACAA GGATTCCCCA CTGTCAAGTT GGAAGGTGTG 
AGTGAATTAG TTGTGCTGCA GGTGTTTGTA GCAAATGATG CAGGCAGAGT GAAGCCTCAC 
GGTTTCTACC AGGCATGCAG AGTGACAGGA CGCAACACCA CTGCTTGCAA AGAGGTGGAC 
ATAGAAGGCA CCATAGTTAT TGAGATTCCT CTGGAGCCCA GCAATGACAT GACACTTGCG 
GTGGACTGTG TAGGAATTTT GAAGCTCCGT AATGCAGATG TCGAAGCCCG CATTGGTGTT 
GCAGGATCCA AGAAGAAGAG TACACGAGCC AGGTTGGCCT TCAGAGTCAG CATCCCCCAG 
CCTGATGGAT CAACCCTGAC TTTACAGGTT CCCTCATCGC CGATCCTCTG CACTCAGCCA 
GCAGGAGTGC CAGAGATCCT GAGAAAGAGT CTCCACAGCT GCTCAGTGAA AGGAGGCGAG 
GAAGTTTTTA TAATCGGAAA GAACTTCCTT AAAGGAACCA AAGTTATTTT TCAGGAGAAC 
ATTACAGATG ATAATTCCTG GCAAGCCGAA GCAAAGATTG ACATGGATCT TTTCCATCAG 
AATCACTTGA TAGTGACAGT CCCTCCGTTT CACAACCAGT CAATCACTTC TCCTGTTTCT 
GTGGGTATTT TTGTGATGAC CAATGCCGGT AGATCACATG AAGCCCAACC CTTCACCTAC 
ACTCCAGACT CGGCTGATAA CTCAAATGTC CTGACAGTAA AAACGGAGGG GATATCCATG 
GTCAAGACCT GCTTATTTGA TACCCCGATC AAATCTATGC CCTCTGAACA AACAGAAATC 
TCTGACCAGA CATCCAAACG GCAAGAGGAC ACACCTATGG AAGTGTCTAG CAATCCACCA 
TCCACAGAGG TCTTCAAATC ATCCCCTGAT CCACTCATCG CAGTGCAGCA GACTCTGGAC 
CTTGGCTCTA GTTCTCATCC AGGTGGAGAG TCCTTTCAGA GAACAATGCC TTTACCATCA 
GAAGATGTGG ACCTTCCCCA GGCACCCCCG GTCTTCCCTA GCTTAGAGTC CCTCAGCACG 
ATACAAAAGC AAGAAATTGC CCCAACAACC TCCTTCTCTG TGTCAGGAGA CACCACAATA 
CCACCTGTGA CACCAGAAGT CCCCCAGCAG TTCCTTAGAG ACCCCCAGGA AAGCTTGTCA 
CCTGAGAGTT CCAGTAGTAG TGCAGGGGTG GTGGTTGTGG CCATGCCACA GATAGCACCT 
TCCTCTCAGG CACAGCCACA GCAGTCCCAG GTCTCCCTCT TTCCCCAGGA AGAGGTGGCC 
CAGCTGGAAA GGGCAGTTAG AGAACTCCAG GGGGGAAGTA ACACCACGCT CCAGCAGGTG 
TTAGAAGCAG CTGTAGCTCA GCAGCAGCTA AATTCTGTGC TGTATAGCCC CACACCTTCT 
GCAGAATCCC TTCAGCAGCA TGTCCAGGAG AACATGAACA GCCTTAGACT GGGGAGTACA 
GATAATTCAC TGTCAGCACA GCAACAGCTA CAGATACAAC AACAGGAGCA GCAACAACAA 
ATACAACAGC AGTTTCAACA ACAGCAACAA ATACTTGGAA ACCTTCAGCA GCAACAGCAG 
CAAGTCATCA ACAACATACA GATGCAACAG CAACTTATAT TACAGCCACA AGAGCAACAG 
CAAATTATGG AGAATATTCA ACAACAGCAA TTGCAACAAA ATCAGCAACA GCAAGTTCTA 
AACAATATTC AGCTTCAGGA TCAGCAACAA CAAAATCATA TTCTTACCAA TTTACAGCAA 
CATCAGCTGC AACAACAGCA GCAGCAGCAA AATCAAGCGT TGAGCAACCT ACAACAGCAA 
CAGCAGCAAC TTCAAGAGCA GCAGGTTTTG GAAAATTTAC AGCAACACCT TCAGGCCAGC 
TCTCTCCTTC AACAGGCTGG AGAACTGCTT ACTATCCAGA CTAGCTTCCC AACACAACCT 
CCTTCACACA CATCCCCCCC ACAGCAACTC TTCCAGTCGC CTAGGCCCAT GGCCGAAACC 
CAGGGGTCCC AACAGCAGGT CCAGGCTGCC CTCATCCAGA ATACCCTGAC CGTACTGACT 
AGTGGCAGTC TCAACTCAGA GCAGCAGTCC ACAGGATCAA CCCTATACCT GACGCCGAAC 
CATCAGCCCC AGCAACAGCA GCAGCTGGCA TTCATCTCCT CCATGGAGAC ATCAGCCAGC 
CAACATCAGT CTGTTTCAAT GTTTCAAAAT CAACCGCAGT CTCAAATGCA GCAACAGAGC 
ACCCCAATGG ATCAGCAGCA GTCCCCACAG CAGAACCAAC AACAACCAGC ACAGCTTCCA 
ATGGGCCAGC AGGGTTCCTT ATTTCAAAGT ATTCCCAATC ACTCACAGGG CAACCCTGCA 
CCACAGCAAA CAGGTCTGCT TCTCTGCACC ACAGATCTGA ACCCACAGGC TATCGCCCCT 
ACTATTCTCT TTAGCACCCA GACCCAAGGC CCTTCCCCTA TGGGGACCAT TAGCGTTGGA 
ATCACCCAGT CAGATACAGC AGAGCCCATG TCCTTCCAAG ACCAGAGCTC TTCGGGCAGC 
AACTCAACAA CCACTGAGAA CCAACAACAG AGCTTGTTCC AGGAACAGCA GCCAATGCAA 
GTGGGCCCAA GTTCAAGCTC CGTCCAAAAC AATCAACCTG TGGAGCTGTT CATACCACAG 
ACAAATCTGT CCAGCTTGCA GAGTACTATT GCCTCACAGG AGCTCAACAA CCAGGCTCCA 
GCCCCCGCCA CAACCATCTT TGTGGTGCAG GGTGGTGTGG GTGTGGTAGC CAGCCCTGGT 
CAGCAGCCCC CAGAGCAGCT TTTCCAGACA TCTGTGGGTG GCAATGTGGC TTCTCAAGGA 
CAGGCCAGTC TGTTTGTTTT TGGCATTCAG AATGACTCAT CCCAGCTGCT CACTTCTTCT 
GGACCCAGCC AACCTGCGCA GAACCAAATC CCGAACTCCG GTCACATGCA GACTCTGCTG 
GATCAGCCCA TGGCCCAGGC TGCCTCCCCA ATCCCAGCTA CCATGCATGG CAGCTTGCAG 
AACACCCTTC AGGCACAAAT GCAGACCACC TTGGAGAACG CAATGCAGGC AAACACACAA 
ACAGCCCTTC AGTCCAGTTT ACAAGGAAAC ATAGAAGCGA GTCTACCAAC ACCAATGCAG 
ACCAGTCTAC AGACACAGAT ACAGAGCAGC TTACAGAATC AACTGCAGGC ATCCATATCA 
ACATCGTCCA GCATGGATAA AATTGAGGAC CTACTGGAAA GCCTACAGAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101484875 ( XM_012919595.1 ) cDNA ORF clone, Maylandia zebra(zebra mbuna) -> XP_012775049.1 Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X4, mRNA.

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CloneID	OMa33420
Clone ID Related Accession (Same CDS sequence)	XM_012919595.1
Accession Version	XM_012919595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Maylandia zebra(zebra mbuna)
Product	nuclear factor of activated T-cells 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014447717.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Maylandia zebra Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGGTG CGGAGGGTGC CCCCAACAGA GGCAGCAGTG GAGCAAACGC TACAGTAGGG 
GATGTAGGGC CGGGAGGGTT GCCGGGGCTA GGAGTCCAGC AGCCCCAGAA CACTCCATCA 
AAGCGAAGGA CTGTGTTGAG CATATCCCCA CCACCCGAGG ATCTGTTTGA TGACAGCCAG 
ATGTCTTGCC AAGAGGAACC TACCATATCT GGTGCACCAG GTCCCGACTC AGAGCAAAGC 
AGCAGCATGT GGGCCGATGA GTCTGTCTCG AACTTCAGTT TGATCAGCTC AATCTCCTAC 
AATGACAACA CGGAGGTGCC ACGCAAGTCT AGAAAACGCA CTCCTCGCCA GCGGCCCGGT 
CCCAAGCCTG CTCCTCCAGA AGACAGCATG GATGTGTTTG ATGCTGACAG CGCCAAAGCC 
CCTCACTATG TGCTATCCCA GCTGGGCACA GATAAGACCA GTTCCATATC CAGCTCTCTC 
GAGTCAGGAA CTGCAGTGAA AGGTGGGTCA CTGTCTGCTC AGTTCCCTCA GAGGAGTGAT 
GGGAAGGAGC TAAAGATCCT AGTGCAGCCA GAGACCCAGC ACAGAGCTCG CTATCTGACT 
GAGGGCAGCA GAGGTTCTGT CAAAGATCGT ACACAACAAG GATTCCCCAC TGTCAAGTTG 
GAAGGTGTGA GTGAATTAGT TGTGCTGCAG GTGTTTGTAG CAAATGATGC AGGCAGAGTG 
AAGCCTCACG GTTTCTACCA GGCATGCAGA GTGACAGGAC GCAACACCAC TGCTTGCAAA 
GAGGTGGACA TAGAAGGCAC CATAGTTATT GAGATTCCTC TGGAGCCCAG CAATGACATG 
ACACTTGCGG TGGACTGTGT AGGAATTTTG AAGCTCCGTA ATGCAGATGT CGAAGCCCGC 
ATTGGTGTTG CAGGATCCAA GAAGAAGAGT ACACGAGCCA GGTTGGCCTT CAGAGTCAGC 
ATCCCCCAGC CTGATGGATC AACCCTGACT TTACAGGTTC CCTCATCGCC GATCCTCTGC 
ACTCAGCCAG CAGGAGTGCC AGAGATCCTG AGAAAGAGTC TCCACAGCTG CTCAGTGAAA 
GGAGGCGAGG AAGTTTTTAT AATCGGAAAG AACTTCCTTA AAGGAACCAA AGTTATTTTT 
CAGGAGAACA TTACAGATGA TAATTCCTGG CAAGCCGAAG CAAAGATTGA CATGGATCTT 
TTCCATCAGA ATCACTTGAT AGTGACAGTC CCTCCGTTTC ACAACCAGTC AATCACTTCT 
CCTGTTTCTG TGGGTATTTT TGTGATGACC AATGCCGGTA GATCACATGA AGCCCAACCC 
TTCACCTACA CTCCAGACTC GGCTGATAAC TCAAATGTCC TGACAGTAAA AACGGAGGGG 
ATATCCATGG TCAAGACCTG CTTATTTGAT ACCCCGATCA AATCTATGCC CTCTGAACAA 
ACAGAAATCT CTGACCAGAC ATCCAAACGG CAAGAGGACA CACCTATGGA AGTGTCTAGC 
AATCCACCAT CCACAGAGGT CTTCAAATCA TCCCCTGATC CACTCATCGC AGTGCAGCAG 
ACTCTGGACC TTGGCTCTAG TTCTCATCCA GGTGGAGAGT CCTTTCAGAG AACAATGCCT 
TTACCATCAG AAGATGTGGA CCTTCCCCAG GCACCCCCGG TCTTCCCTAG CTTAGAGTCC 
CTCAGCACGA TACAAAAGCA AGAAATTGCC CCAACAACCT CCTTCTCTGT GTCAGGAGAC 
ACCACAATAC CACCTGTGAC ACCAGAAGTC CCCCAGCAGT TCCTTAGAGA CCCCCAGGAA 
AGCTTGTCAC CTGAGAGTTC CAGTAGTAGT GCAGGGGTGG TGGTTGTGGC CATGCCACAG 
ATAGCACCTT CCTCTCAGGC ACAGCCACAG CAGTCCCAGG TCTCCCTCTT TCCCCAGGAA 
GAGGTGGCCC AGCTGGAAAG GGCAGTTAGA GAACTCCAGG GGGGAAGTAA CACCACGCTC 
CAGCAGGTGT TAGAAGCAGC TGTAGCTCAG CAGCAGCTAA ATTCTGTGCT GTATAGCCCC 
ACACCTTCTG CAGAATCCCT TCAGCAGCAT GTCCAGGAGA ACATGAACAG CCTTAGACTG 
GGGAGTACAG ATAATTCACT GTCAGCACAG CAACAGCTAC AGATACAACA ACAGGAGCAG 
CAACAACAAA TACAACAGCA GTTTCAACAA CAGCAACAAA TACTTGGAAA CCTTCAGCAG 
CAACAGCAGC AAGTCATCAA CAACATACAG ATGCAACAGC AACTTATATT ACAGCCACAA 
GAGCAACAGC AAATTATGGA GAATATTCAA CAACAGCAAT TGCAACAAAA TCAGCAACAG 
CAAGTTCTAA ACAATATTCA GCTTCAGGAT CAGCAACAAC AAAATCATAT TCTTACCAAT 
TTACAGCAAC ATCAGCTGCA ACAACAGCAG CAGCAGCAAA ATCAAGCGTT GAGCAACCTA 
CAACAGCAAC AGCAGCAACT TCAAGAGCAG CAGGTTTTGG AAAATTTACA GCAACACCTT 
CAGGCCAGCT CTCTCCTTCA ACAGGCTGGA GAACTGCTTA CTATCCAGAC TAGCTTCCCA 
ACACAACCTC CTTCACACAC ATCCCCCCCA CAGCAACTCT TCCAGTCGCC TAGGCCCATG 
GCCGAAACCC AGGGGTCCCA ACAGCAGGTC CAGGCTGCCC TCATCCAGAA TACCCTGACC 
GTACTGACTA GTGGCAGTCT CAACTCAGAG CAGCAGTCCA CAGGATCAAC CCTATACCTG 
ACGCCGAACC ATCAGCCCCA GCAACAGCAG CAGCTGGCAT TCATCTCCTC CATGGAGACA 
TCAGCCAGCC AACATCAGTC TGTTTCAATG TTTCAAAATC AACCGCAGTC TCAAATGCAG 
CAACAGAGCA CCCCAATGGA TCAGCAGCAG TCCCCACAGC AGAACCAACA ACAACCAGCA 
CAGCTTCCAA TGGGCCAGCA GGGTTCCTTA TTTCAAAGTA TTCCCAATCA CTCACAGGGC 
AACCCTGCAC CACAGCAAAC AGGTCTGCTT CTCTGCACCA CAGATCTGAA CCCACAGGCT 
ATCGCCCCTA CTATTCTCTT TAGCACCCAG ACCCAAGGCC CTTCCCCTAT GGGGACCATT 
AGCGTTGGAA TCACCCAGTC AGATACAGCA GAGCCCATGT CCTTCCAAGA CCAGAGCTCT 
TCGGGCAGCA ACTCAACAAC CACTGAGAAC CAACAACAGA GCTTGTTCCA GGAACAGCAG 
CCAATGCAAG TGGGCCCAAG TTCAAGCTCC GTCCAAAACA ATCAACCTGT GGAGCTGTTC 
ATACCACAGA CAAATCTGTC CAGCTTGCAG AGTACTATTG CCTCACAGGA GCTCAACAAC 
CAGGCTCCAG CCCCCGCCAC AACCATCTTT GTGGTGCAGG GTGGTGTGGG TGTGGTAGCC 
AGCCCTGGTC AGCAGCCCCC AGAGCAGCTT TTCCAGACAT CTGTGGGTGG CAATGTGGCT 
TCTCAAGGAC AGGCCAGTCT GTTTGTTTTT GGCATTCAGA ATGACTCATC CCAGCTGCTC 
ACTTCTTCTG GACCCAGCCA ACCTGCGCAG AACCAAATCC CGAACTCCGG TCACATGCAG 
ACTCTGCTGG ATCAGCCCAT GGCCCAGGCT GCCTCCCCAA TCCCAGCTAC CATGCATGGC 
AGCTTGCAGA ACACCCTTCA GGCACAAATG CAGACCACCT TGGAGAACGC AATGCAGGCA 
AACACACAAA CAGCCCTTCA GTCCAGTTTA CAAGGAAACA TAGAAGCGAG TCTACCAACA 
CCAATGCAGA CCAGTCTACA GACACAGATA CAGAGCAGCT TACAGAATCA ACTGCAGGCA 
TCCATATCAA CATCGTCCAG CATGGATAAA ATTGAGGACC TACTGGAAAG CCTACAGAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012775049.1
CDS	302..4207
Translation	MQGAEGAPNRGSSGANATVGDVGPGGLPGLGVQQPQNTPSKRRTVLSISPPPEDLFDDSQMSCQEEPTISGAPGPDSEQSSSMWADESVSNFSLISSISYNDNTEVPRKSRKRTPRQRPGPKPAPPEDSMDVFDADSAKAPHYVLSQLGTDKTSSISSSLESGTAVKGGSLSAQFPQRSDGKELKILVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGVSELVVLQVFVANDAGRVKPHGFYQACRVTGRNTTACKEVDIEGTIVIEIPLEPSNDMTLAVDCVGILKLRNADVEARIGVAGSKKKSTRARLAFRVSIPQPDGSTLTLQVPSSPILCTQPAGVPEILRKSLHSCSVKGGEEVFIIGKNFLKGTKVIFQENITDDNSWQAEAKIDMDLFHQNHLIVTVPPFHNQSITSPVSVGIFVMTNAGRSHEAQPFTYTPDSADNSNVLTVKTEGISMVKTCLFDTPIKSMPSEQTEISDQTSKRQEDTPMEVSSNPPSTEVFKSSPDPLIAVQQTLDLGSSSHPGGESFQRTMPLPSEDVDLPQAPPVFPSLESLSTIQKQEIAPTTSFSVSGDTTIPPVTPEVPQQFLRDPQESLSPESSSSSAGVVVVAMPQIAPSSQAQPQQSQVSLFPQEEVAQLERAVRELQGGSNTTLQQVLEAAVAQQQLNSVLYSPTPSAESLQQHVQENMNSLRLGSTDNSLSAQQQLQIQQQEQQQQIQQQFQQQQQILGNLQQQQQQVINNIQMQQQLILQPQEQQQIMENIQQQQLQQNQQQQVLNNIQLQDQQQQNHILTNLQQHQLQQQQQQQNQALSNLQQQQQQLQEQQVLENLQQHLQASSLLQQAGELLTIQTSFPTQPPSHTSPPQQLFQSPRPMAETQGSQQQVQAALIQNTLTVLTSGSLNSEQQSTGSTLYLTPNHQPQQQQQLAFISSMETSASQHQSVSMFQNQPQSQMQQQSTPMDQQQSPQQNQQQPAQLPMGQQGSLFQSIPNHSQGNPAPQQTGLLLCTTDLNPQAIAPTILFSTQTQGPSPMGTISVGITQSDTAEPMSFQDQSSSGSNSTTTENQQQSLFQEQQPMQVGPSSSSVQNNQPVELFIPQTNLSSLQSTIASQELNNQAPAPATTIFVVQGGVGVVASPGQQPPEQLFQTSVGGNVASQGQASLFVFGIQNDSSQLLTSSGPSQPAQNQIPNSGHMQTLLDQPMAQAASPIPATMHGSLQNTLQAQMQTTLENAMQANTQTALQSSLQGNIEASLPTPMQTSLQTQIQSSLQNQLQASISTSSSMDKIEDLLESLQKQ

Target ORF information:

RefSeq Version	XM_012919595.1
Organism	Maylandia zebra(zebra mbuna)
Definition	Maylandia zebra nuclear factor of activated T-cells 5 (LOC101484875), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012919595.1

ORF Insert Sequence:

ATGCAAGGTG CGGAGGGTGC CCCCAACAGA GGCAGCAGTG GAGCAAACGC TACAGTAGGG 
GATGTAGGGC CGGGAGGGTT GCCGGGGCTA GGAGTCCAGC AGCCCCAGAA CACTCCATCA 
AAGCGAAGGA CTGTGTTGAG CATATCCCCA CCACCCGAGG ATCTGTTTGA TGACAGCCAG 
ATGTCTTGCC AAGAGGAACC TACCATATCT GGTGCACCAG GTCCCGACTC AGAGCAAAGC 
AGCAGCATGT GGGCCGATGA GTCTGTCTCG AACTTCAGTT TGATCAGCTC AATCTCCTAC 
AATGACAACA CGGAGGTGCC ACGCAAGTCT AGAAAACGCA CTCCTCGCCA GCGGCCCGGT 
CCCAAGCCTG CTCCTCCAGA AGACAGCATG GATGTGTTTG ATGCTGACAG CGCCAAAGCC 
CCTCACTATG TGCTATCCCA GCTGGGCACA GATAAGACCA GTTCCATATC CAGCTCTCTC 
GAGTCAGGAA CTGCAGTGAA AGGTGGGTCA CTGTCTGCTC AGTTCCCTCA GAGGAGTGAT 
GGGAAGGAGC TAAAGATCCT AGTGCAGCCA GAGACCCAGC ACAGAGCTCG CTATCTGACT 
GAGGGCAGCA GAGGTTCTGT CAAAGATCGT ACACAACAAG GATTCCCCAC TGTCAAGTTG 
GAAGGTGTGA GTGAATTAGT TGTGCTGCAG GTGTTTGTAG CAAATGATGC AGGCAGAGTG 
AAGCCTCACG GTTTCTACCA GGCATGCAGA GTGACAGGAC GCAACACCAC TGCTTGCAAA 
GAGGTGGACA TAGAAGGCAC CATAGTTATT GAGATTCCTC TGGAGCCCAG CAATGACATG 
ACACTTGCGG TGGACTGTGT AGGAATTTTG AAGCTCCGTA ATGCAGATGT CGAAGCCCGC 
ATTGGTGTTG CAGGATCCAA GAAGAAGAGT ACACGAGCCA GGTTGGCCTT CAGAGTCAGC 
ATCCCCCAGC CTGATGGATC AACCCTGACT TTACAGGTTC CCTCATCGCC GATCCTCTGC 
ACTCAGCCAG CAGGAGTGCC AGAGATCCTG AGAAAGAGTC TCCACAGCTG CTCAGTGAAA 
GGAGGCGAGG AAGTTTTTAT AATCGGAAAG AACTTCCTTA AAGGAACCAA AGTTATTTTT 
CAGGAGAACA TTACAGATGA TAATTCCTGG CAAGCCGAAG CAAAGATTGA CATGGATCTT 
TTCCATCAGA ATCACTTGAT AGTGACAGTC CCTCCGTTTC ACAACCAGTC AATCACTTCT 
CCTGTTTCTG TGGGTATTTT TGTGATGACC AATGCCGGTA GATCACATGA AGCCCAACCC 
TTCACCTACA CTCCAGACTC GGCTGATAAC TCAAATGTCC TGACAGTAAA AACGGAGGGG 
ATATCCATGG TCAAGACCTG CTTATTTGAT ACCCCGATCA AATCTATGCC CTCTGAACAA 
ACAGAAATCT CTGACCAGAC ATCCAAACGG CAAGAGGACA CACCTATGGA AGTGTCTAGC 
AATCCACCAT CCACAGAGGT CTTCAAATCA TCCCCTGATC CACTCATCGC AGTGCAGCAG 
ACTCTGGACC TTGGCTCTAG TTCTCATCCA GGTGGAGAGT CCTTTCAGAG AACAATGCCT 
TTACCATCAG AAGATGTGGA CCTTCCCCAG GCACCCCCGG TCTTCCCTAG CTTAGAGTCC 
CTCAGCACGA TACAAAAGCA AGAAATTGCC CCAACAACCT CCTTCTCTGT GTCAGGAGAC 
ACCACAATAC CACCTGTGAC ACCAGAAGTC CCCCAGCAGT TCCTTAGAGA CCCCCAGGAA 
AGCTTGTCAC CTGAGAGTTC CAGTAGTAGT GCAGGGGTGG TGGTTGTGGC CATGCCACAG 
ATAGCACCTT CCTCTCAGGC ACAGCCACAG CAGTCCCAGG TCTCCCTCTT TCCCCAGGAA 
GAGGTGGCCC AGCTGGAAAG GGCAGTTAGA GAACTCCAGG GGGGAAGTAA CACCACGCTC 
CAGCAGGTGT TAGAAGCAGC TGTAGCTCAG CAGCAGCTAA ATTCTGTGCT GTATAGCCCC 
ACACCTTCTG CAGAATCCCT TCAGCAGCAT GTCCAGGAGA ACATGAACAG CCTTAGACTG 
GGGAGTACAG ATAATTCACT GTCAGCACAG CAACAGCTAC AGATACAACA ACAGGAGCAG 
CAACAACAAA TACAACAGCA GTTTCAACAA CAGCAACAAA TACTTGGAAA CCTTCAGCAG 
CAACAGCAGC AAGTCATCAA CAACATACAG ATGCAACAGC AACTTATATT ACAGCCACAA 
GAGCAACAGC AAATTATGGA GAATATTCAA CAACAGCAAT TGCAACAAAA TCAGCAACAG 
CAAGTTCTAA ACAATATTCA GCTTCAGGAT CAGCAACAAC AAAATCATAT TCTTACCAAT 
TTACAGCAAC ATCAGCTGCA ACAACAGCAG CAGCAGCAAA ATCAAGCGTT GAGCAACCTA 
CAACAGCAAC AGCAGCAACT TCAAGAGCAG CAGGTTTTGG AAAATTTACA GCAACACCTT 
CAGGCCAGCT CTCTCCTTCA ACAGGCTGGA GAACTGCTTA CTATCCAGAC TAGCTTCCCA 
ACACAACCTC CTTCACACAC ATCCCCCCCA CAGCAACTCT TCCAGTCGCC TAGGCCCATG 
GCCGAAACCC AGGGGTCCCA ACAGCAGGTC CAGGCTGCCC TCATCCAGAA TACCCTGACC 
GTACTGACTA GTGGCAGTCT CAACTCAGAG CAGCAGTCCA CAGGATCAAC CCTATACCTG 
ACGCCGAACC ATCAGCCCCA GCAACAGCAG CAGCTGGCAT TCATCTCCTC CATGGAGACA 
TCAGCCAGCC AACATCAGTC TGTTTCAATG TTTCAAAATC AACCGCAGTC TCAAATGCAG 
CAACAGAGCA CCCCAATGGA TCAGCAGCAG TCCCCACAGC AGAACCAACA ACAACCAGCA 
CAGCTTCCAA TGGGCCAGCA GGGTTCCTTA TTTCAAAGTA TTCCCAATCA CTCACAGGGC 
AACCCTGCAC CACAGCAAAC AGGTCTGCTT CTCTGCACCA CAGATCTGAA CCCACAGGCT 
ATCGCCCCTA CTATTCTCTT TAGCACCCAG ACCCAAGGCC CTTCCCCTAT GGGGACCATT 
AGCGTTGGAA TCACCCAGTC AGATACAGCA GAGCCCATGT CCTTCCAAGA CCAGAGCTCT 
TCGGGCAGCA ACTCAACAAC CACTGAGAAC CAACAACAGA GCTTGTTCCA GGAACAGCAG 
CCAATGCAAG TGGGCCCAAG TTCAAGCTCC GTCCAAAACA ATCAACCTGT GGAGCTGTTC 
ATACCACAGA CAAATCTGTC CAGCTTGCAG AGTACTATTG CCTCACAGGA GCTCAACAAC 
CAGGCTCCAG CCCCCGCCAC AACCATCTTT GTGGTGCAGG GTGGTGTGGG TGTGGTAGCC 
AGCCCTGGTC AGCAGCCCCC AGAGCAGCTT TTCCAGACAT CTGTGGGTGG CAATGTGGCT 
TCTCAAGGAC AGGCCAGTCT GTTTGTTTTT GGCATTCAGA ATGACTCATC CCAGCTGCTC 
ACTTCTTCTG GACCCAGCCA ACCTGCGCAG AACCAAATCC CGAACTCCGG TCACATGCAG 
ACTCTGCTGG ATCAGCCCAT GGCCCAGGCT GCCTCCCCAA TCCCAGCTAC CATGCATGGC 
AGCTTGCAGA ACACCCTTCA GGCACAAATG CAGACCACCT TGGAGAACGC AATGCAGGCA 
AACACACAAA CAGCCCTTCA GTCCAGTTTA CAAGGAAACA TAGAAGCGAG TCTACCAACA 
CCAATGCAGA CCAGTCTACA GACACAGATA CAGAGCAGCT TACAGAATCA ACTGCAGGCA 
TCCATATCAA CATCGTCCAG CATGGATAAA ATTGAGGACC TACTGGAAAG CCTACAGAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101484875 cDNA ORF clone, Maylandia zebra(zebra mbuna)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム