TGS1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	TGS1
Entrez Gene ID	108506352
Full Name	trimethylguanosine synthase 1
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017833284.1	XP_017688773.1	trimethylguanosine synthase isoform X1
XM_017833296.1	XP_017688785.1	trimethylguanosine synthase isoform X2

Pathways from BioSystems
Gene Ontology

The following TGS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TGS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe50079	XM_017833284.1 Latest version!	Lepidothrix coronata trimethylguanosine synthase 1 (TGS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OLe50080	XM_017833296.1 Latest version!	Lepidothrix coronata trimethylguanosine synthase 1 (TGS1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TGS1 ( XM_017833284.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017688773.1 Lepidothrix coronata trimethylguanosine synthase 1 (TGS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLe50079
Clone ID Related Accession (Same CDS sequence)	XM_017833284.1
Accession Version	XM_017833284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	trimethylguanosine synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690186.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGG ACCCGGGCGG GATGCTGGTG GCGGAGCTGG TGCTGCGGGC GGGGGCGGCT 
CGCAGCATCC TCTGCCTCTG CTCCCGCGCC TTCGTCGAAG ATCGAAAATT ATATAAACTG 
GGATTAAAAG GATTTTATGT CAAAGATGAC AATGACAGTA CAGCAGGGGA AGAAGAAGCG 
TCTGAGGAGG AGAACCATTG TCCCAATGTG AGATTTAAGG TGGATACTAA TCATGCGCTG 
GACCCAGAAG AAGTTGAACT AGACTCAGAA GCTGAGCTTA TGAAGAGCAT GGGGTTGCCT 
GTTCAGTTTG GTGGGCAGTC AGCCCAGAGA GACTTTGTGG CAACAGAAAA TTATAAAAAG 
AGAAATAATG TGAAATTTAT GAAAAAGAAA AAGAAGAAGA AAAAAGAGTT ACAGCAAAAA 
CATGAGGATA AAAGGGGACA GGAATGCCAG GATGAAGGTT GTGGTGGTAG TATCCAGGCC 
ATTTCTAGTG AGCTGGCCCT AGCTACAGAG CAATGTGAGA TGAGCAGTAA ATCTCAGATT 
GTAGCTGAAG AGAACTGTGA AAGTTCAGAA AGCCTTGCAA ATGAGGCTTT ATCCAGTGAA 
CTTCAGGAAA AATGGGAGAA GTACTGGAGC GAGTATGGAG AGGGCCTTCT TTGGCAAAGT 
TGGTTGCAGA AGCATCAAGA GGTCTCATCA TCTGAAGCCA TCACAGCCTC TGAGCCTTGG 
AATAGTCTGG ACACCAAGGG AGAATGGGAG CAGCATTATA ATGAACTGTA CTGGTATTAT 
TGGGAACAGT TTCAGTACTG GACAAGTCAA GGATGGACTA TTGAGAGCTC ACATGGGGAC 
AAGGTGGAAG TTAATGGGGT TACGCAGGAG ACTGGTCCTT TGGGAGAAAT GAACTTGGTT 
AGCCAAGGGC CTGAATGCAG TGAAGTGCTG AGCTTGGAGC TGTCTCCCTC TAACACCAGA 
AGTGATGAAA CACTCCCTTC AAGTGCTGAG CTGCACAGTG AAATCATCTC TGGAATTTGT 
AATTTAAATC TGAATTTAAA GGAAGTGGAG CAGAGCAGCG CAGCTCTAAC AGTAGCCCAC 
CAAGGTCATC AAGAGCACAG TTTGTCTGAC AGTGGAAGTC AGAAGGAGCC CTGTGATGGA 
GGAACCAGGA AAAGGAGCGC ATCTTGTGAA AATAAAAGTG TTAACCAGTC AGGTTCACAG 
GAGTCATGTA GCCTGAATTC AAATGAAAAA GAGCAGTTGC TGCTTCATGA CCAAGATGAA 
GATGAGGACG AAGAGCCTCC TGAATACAGA CATGTCAAAG TCAAGAGAAG TCATGAACTG 
GATGTGGATG AGAACCCAGT GGAAGATCCT GAAGAAACCT GCTCTGTGTT GGGTTTCAAG 
TGTGGAACAG GACAAAAGTA TGGTGGAATT CCAGATTTCA CCCACAGAAG TGTGCAGTAC 
TTGGAGAAAA AAGCAAAACT CAAGTCCAAA TACTTGGACA TGCGTAAACC AAGGAAGAGC 
AAAAACACAC ACATCTTCTT TACAGAGGAA TCTGAAATGA CATGCAAAAA AAGCAAAACT 
TTGAAGAAGG TGGAAATGTT CCTGAAGCGG GTTAGTAAAC CCGAGGAGGA AGCTACATCC 
CAGACAGCCG TCCCTCAGAG TAAATCGGAG GCATTGTCTG AGAGCAGCAG CGACTCAGAG 
GGTCAGGAAG GCCTTACTGC CACACAGACT GTGATGCTGA ATGCTGAAAA CCAAAAGCCA 
CCATCTTCCA GTGCAGCCTG CACAGAACCT GTAGGGAGCA ACCTTGAGGA GGAGGCACAG 
GAAGCTGCAG AGAGTGGCTC CAATGAGCAG GGTGCAGCGG GGCCAGAGCG TGGTGGTGGG 
CGGCAGCTTG TCTCTCTGGA TATTCCCGAT TACCTCCAGG CAAACACAGA GGACGTCAGC 
CAAGCTGTAG ATGAAAAAAT AACCACAAAG AAGAAGGAGA AGAAAAGAAG AAGGAATAAA 
ATTGCACTGA GATCTATACC TGCTGAGATT GCTGCTGATC CAGAGCTGGT CAAGTACTGG 
GCACAACGCT ACCGGCTGTT CTCTCGGTTT GACGAGGGAA TTAAACTGGA CAGAGAGGGT 
TGGTTTTCTG TTACTCCTGA GAAAATAGCT GAGCATATTG CAGTCCGTGT TAGTCAGTCA 
TTCAACTGCG ATATCATAGT GGATGCGTTC TGTGGGGTTG GAGGAAACGC TATTCAGTTT 
GCATTGACCT CAAAAAGAGT GATCGCTATC GACATCGATC CCGAGAAGCT CAGCCTGGCG 
CGCAACAACG CAGAGGTGTA CGGTGTGGCG GATCAGATCG AGTTCGTGTG CGGGGACTTC 
ATGGTGCTGG CTGCCGGCCT GCAGGCAGAT GTGGTGTTCC TCAGCCCACC CTGGGGGGGA 
CCTGATTATG CCACTGCCGA AATCTTCGAT ATCCAGACCA TGATTTGCCC AGATGGATTT 
GAAATTTTCA GGCTCTCCAA GAAGATCACC AACAACATTG TGTATTTTCT ACCTCGGAAT 
GCTGATCTTG ATCAGGTGGC TTCCTTAGCA GGCCCAGGAG GGAAAGTTGA AATAGAACAA 
AATTTTCTCA ATAACAAACT GAAGACGATA ACAGCTTATT TTGGTGATCT AATAAGACAT 
GACATCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017688773.1
CDS	44..2695
Translation	MVQDPGGMLVAELVLRAGAARSILCLCSRAFVEDRKLYKLGLKGFYVKDDNDSTAGEEEASEEENHCPNVRFKVDTNHALDPEEVELDSEAELMKSMGLPVQFGGQSAQRDFVATENYKKRNNVKFMKKKKKKKKELQQKHEDKRGQECQDEGCGGSIQAISSELALATEQCEMSSKSQIVAEENCESSESLANEALSSELQEKWEKYWSEYGEGLLWQSWLQKHQEVSSSEAITASEPWNSLDTKGEWEQHYNELYWYYWEQFQYWTSQGWTIESSHGDKVEVNGVTQETGPLGEMNLVSQGPECSEVLSLELSPSNTRSDETLPSSAELHSEIISGICNLNLNLKEVEQSSAALTVAHQGHQEHSLSDSGSQKEPCDGGTRKRSASCENKSVNQSGSQESCSLNSNEKEQLLLHDQDEDEDEEPPEYRHVKVKRSHELDVDENPVEDPEETCSVLGFKCGTGQKYGGIPDFTHRSVQYLEKKAKLKSKYLDMRKPRKSKNTHIFFTEESEMTCKKSKTLKKVEMFLKRVSKPEEEATSQTAVPQSKSEALSESSSDSEGQEGLTATQTVMLNAENQKPPSSSAACTEPVGSNLEEEAQEAAESGSNEQGAAGPERGGGRQLVSLDIPDYLQANTEDVSQAVDEKITTKKKEKKRRRNKIALRSIPAEIAADPELVKYWAQRYRLFSRFDEGIKLDREGWFSVTPEKIAEHIAVRVSQSFNCDIIVDAFCGVGGNAIQFALTSKRVIAIDIDPEKLSLARNNAEVYGVADQIEFVCGDFMVLAAGLQADVVFLSPPWGGPDYATAEIFDIQTMICPDGFEIFRLSKKITNNIVYFLPRNADLDQVASLAGPGGKVEIEQNFLNNKLKTITAYFGDLIRHDIS

Target ORF information:

RefSeq Version	XM_017833284.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata trimethylguanosine synthase 1 (TGS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017833284.1

ORF Insert Sequence:

ATGGTGCAGG ACCCGGGCGG GATGCTGGTG GCGGAGCTGG TGCTGCGGGC GGGGGCGGCT 
CGCAGCATCC TCTGCCTCTG CTCCCGCGCC TTCGTCGAAG ATCGAAAATT ATATAAACTG 
GGATTAAAAG GATTTTATGT CAAAGATGAC AATGACAGTA CAGCAGGGGA AGAAGAAGCG 
TCTGAGGAGG AGAACCATTG TCCCAATGTG AGATTTAAGG TGGATACTAA TCATGCGCTG 
GACCCAGAAG AAGTTGAACT AGACTCAGAA GCTGAGCTTA TGAAGAGCAT GGGGTTGCCT 
GTTCAGTTTG GTGGGCAGTC AGCCCAGAGA GACTTTGTGG CAACAGAAAA TTATAAAAAG 
AGAAATAATG TGAAATTTAT GAAAAAGAAA AAGAAGAAGA AAAAAGAGTT ACAGCAAAAA 
CATGAGGATA AAAGGGGACA GGAATGCCAG GATGAAGGTT GTGGTGGTAG TATCCAGGCC 
ATTTCTAGTG AGCTGGCCCT AGCTACAGAG CAATGTGAGA TGAGCAGTAA ATCTCAGATT 
GTAGCTGAAG AGAACTGTGA AAGTTCAGAA AGCCTTGCAA ATGAGGCTTT ATCCAGTGAA 
CTTCAGGAAA AATGGGAGAA GTACTGGAGC GAGTATGGAG AGGGCCTTCT TTGGCAAAGT 
TGGTTGCAGA AGCATCAAGA GGTCTCATCA TCTGAAGCCA TCACAGCCTC TGAGCCTTGG 
AATAGTCTGG ACACCAAGGG AGAATGGGAG CAGCATTATA ATGAACTGTA CTGGTATTAT 
TGGGAACAGT TTCAGTACTG GACAAGTCAA GGATGGACTA TTGAGAGCTC ACATGGGGAC 
AAGGTGGAAG TTAATGGGGT TACGCAGGAG ACTGGTCCTT TGGGAGAAAT GAACTTGGTT 
AGCCAAGGGC CTGAATGCAG TGAAGTGCTG AGCTTGGAGC TGTCTCCCTC TAACACCAGA 
AGTGATGAAA CACTCCCTTC AAGTGCTGAG CTGCACAGTG AAATCATCTC TGGAATTTGT 
AATTTAAATC TGAATTTAAA GGAAGTGGAG CAGAGCAGCG CAGCTCTAAC AGTAGCCCAC 
CAAGGTCATC AAGAGCACAG TTTGTCTGAC AGTGGAAGTC AGAAGGAGCC CTGTGATGGA 
GGAACCAGGA AAAGGAGCGC ATCTTGTGAA AATAAAAGTG TTAACCAGTC AGGTTCACAG 
GAGTCATGTA GCCTGAATTC AAATGAAAAA GAGCAGTTGC TGCTTCATGA CCAAGATGAA 
GATGAGGACG AAGAGCCTCC TGAATACAGA CATGTCAAAG TCAAGAGAAG TCATGAACTG 
GATGTGGATG AGAACCCAGT GGAAGATCCT GAAGAAACCT GCTCTGTGTT GGGTTTCAAG 
TGTGGAACAG GACAAAAGTA TGGTGGAATT CCAGATTTCA CCCACAGAAG TGTGCAGTAC 
TTGGAGAAAA AAGCAAAACT CAAGTCCAAA TACTTGGACA TGCGTAAACC AAGGAAGAGC 
AAAAACACAC ACATCTTCTT TACAGAGGAA TCTGAAATGA CATGCAAAAA AAGCAAAACT 
TTGAAGAAGG TGGAAATGTT CCTGAAGCGG GTTAGTAAAC CCGAGGAGGA AGCTACATCC 
CAGACAGCCG TCCCTCAGAG TAAATCGGAG GCATTGTCTG AGAGCAGCAG CGACTCAGAG 
GGTCAGGAAG GCCTTACTGC CACACAGACT GTGATGCTGA ATGCTGAAAA CCAAAAGCCA 
CCATCTTCCA GTGCAGCCTG CACAGAACCT GTAGGGAGCA ACCTTGAGGA GGAGGCACAG 
GAAGCTGCAG AGAGTGGCTC CAATGAGCAG GGTGCAGCGG GGCCAGAGCG TGGTGGTGGG 
CGGCAGCTTG TCTCTCTGGA TATTCCCGAT TACCTCCAGG CAAACACAGA GGACGTCAGC 
CAAGCTGTAG ATGAAAAAAT AACCACAAAG AAGAAGGAGA AGAAAAGAAG AAGGAATAAA 
ATTGCACTGA GATCTATACC TGCTGAGATT GCTGCTGATC CAGAGCTGGT CAAGTACTGG 
GCACAACGCT ACCGGCTGTT CTCTCGGTTT GACGAGGGAA TTAAACTGGA CAGAGAGGGT 
TGGTTTTCTG TTACTCCTGA GAAAATAGCT GAGCATATTG CAGTCCGTGT TAGTCAGTCA 
TTCAACTGCG ATATCATAGT GGATGCGTTC TGTGGGGTTG GAGGAAACGC TATTCAGTTT 
GCATTGACCT CAAAAAGAGT GATCGCTATC GACATCGATC CCGAGAAGCT CAGCCTGGCG 
CGCAACAACG CAGAGGTGTA CGGTGTGGCG GATCAGATCG AGTTCGTGTG CGGGGACTTC 
ATGGTGCTGG CTGCCGGCCT GCAGGCAGAT GTGGTGTTCC TCAGCCCACC CTGGGGGGGA 
CCTGATTATG CCACTGCCGA AATCTTCGAT ATCCAGACCA TGATTTGCCC AGATGGATTT 
GAAATTTTCA GGCTCTCCAA GAAGATCACC AACAACATTG TGTATTTTCT ACCTCGGAAT 
GCTGATCTTG ATCAGGTGGC TTCCTTAGCA GGCCCAGGAG GGAAAGTTGA AATAGAACAA 
AATTTTCTCA ATAACAAACT GAAGACGATA ACAGCTTATT TTGGTGATCT AATAAGACAT 
GACATCTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TGS1 ( XM_017833296.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017688785.1 Lepidothrix coronata trimethylguanosine synthase 1 (TGS1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OLe50080
Clone ID Related Accession (Same CDS sequence)	XM_017833296.1
Accession Version	XM_017833296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	trimethylguanosine synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690186.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCAGG ACCCGGGCGG GATGCTGGTG GCGGAGCTGG TGCTGCGGGC GGGGGCGGCT 
CGCAGCATCC TCTGCCTCTG CTCCCGCGCC TTCGTCGAAG ATCGAAAATT ATATAAACTG 
GGATTAAAAG GATTTTATGT CAAAGATGAC AATGACAGTA CAGGGGAAGA AGAAGCGTCT 
GAGGAGGAGA ACCATTGTCC CAATGTGAGA TTTAAGGTGG ATACTAATCA TGCGCTGGAC 
CCAGAAGAAG TTGAACTAGA CTCAGAAGCT GAGCTTATGA AGAGCATGGG GTTGCCTGTT 
CAGTTTGGTG GGCAGTCAGC CCAGAGAGAC TTTGTGGCAA CAGAAAATTA TAAAAAGAGA 
AATAATGTGA AATTTATGAA AAAGAAAAAG AAGAAGAAAA AAGAGTTACA GCAAAAACAT 
GAGGATAAAA GGGGACAGGA ATGCCAGGAT GAAGGTTGTG GTGGTAGTAT CCAGGCCATT 
TCTAGTGAGC TGGCCCTAGC TACAGAGCAA TGTGAGATGA GCAGTAAATC TCAGATTGTA 
GCTGAAGAGA ACTGTGAAAG TTCAGAAAGC CTTGCAAATG AGGCTTTATC CAGTGAACTT 
CAGGAAAAAT GGGAGAAGTA CTGGAGCGAG TATGGAGAGG GCCTTCTTTG GCAAAGTTGG 
TTGCAGAAGC ATCAAGAGGT CTCATCATCT GAAGCCATCA CAGCCTCTGA GCCTTGGAAT 
AGTCTGGACA CCAAGGGAGA ATGGGAGCAG CATTATAATG AACTGTACTG GTATTATTGG 
GAACAGTTTC AGTACTGGAC AAGTCAAGGA TGGACTATTG AGAGCTCACA TGGGGACAAG 
GTGGAAGTTA ATGGGGTTAC GCAGGAGACT GGTCCTTTGG GAGAAATGAA CTTGGTTAGC 
CAAGGGCCTG AATGCAGTGA AGTGCTGAGC TTGGAGCTGT CTCCCTCTAA CACCAGAAGT 
GATGAAACAC TCCCTTCAAG TGCTGAGCTG CACAGTGAAA TCATCTCTGG AATTTGTAAT 
TTAAATCTGA ATTTAAAGGA AGTGGAGCAG AGCAGCGCAG CTCTAACAGT AGCCCACCAA 
GGTCATCAAG AGCACAGTTT GTCTGACAGT GGAAGTCAGA AGGAGCCCTG TGATGGAGGA 
ACCAGGAAAA GGAGCGCATC TTGTGAAAAT AAAAGTGTTA ACCAGTCAGG TTCACAGGAG 
TCATGTAGCC TGAATTCAAA TGAAAAAGAG CAGTTGCTGC TTCATGACCA AGATGAAGAT 
GAGGACGAAG AGCCTCCTGA ATACAGACAT GTCAAAGTCA AGAGAAGTCA TGAACTGGAT 
GTGGATGAGA ACCCAGTGGA AGATCCTGAA GAAACCTGCT CTGTGTTGGG TTTCAAGTGT 
GGAACAGGAC AAAAGTATGG TGGAATTCCA GATTTCACCC ACAGAAGTGT GCAGTACTTG 
GAGAAAAAAG CAAAACTCAA GTCCAAATAC TTGGACATGC GTAAACCAAG GAAGAGCAAA 
AACACACACA TCTTCTTTAC AGAGGAATCT GAAATGACAT GCAAAAAAAG CAAAACTTTG 
AAGAAGGTGG AAATGTTCCT GAAGCGGGTT AGTAAACCCG AGGAGGAAGC TACATCCCAG 
ACAGCCGTCC CTCAGAGTAA ATCGGAGGCA TTGTCTGAGA GCAGCAGCGA CTCAGAGGGT 
CAGGAAGGCC TTACTGCCAC ACAGACTGTG ATGCTGAATG CTGAAAACCA AAAGCCACCA 
TCTTCCAGTG CAGCCTGCAC AGAACCTGTA GGGAGCAACC TTGAGGAGGA GGCACAGGAA 
GCTGCAGAGA GTGGCTCCAA TGAGCAGGGT GCAGCGGGGC CAGAGCGTGG TGGTGGGCGG 
CAGCTTGTCT CTCTGGATAT TCCCGATTAC CTCCAGGCAA ACACAGAGGA CGTCAGCCAA 
GCTGTAGATG AAAAAATAAC CACAAAGAAG AAGGAGAAGA AAAGAAGAAG GAATAAAATT 
GCACTGAGAT CTATACCTGC TGAGATTGCT GCTGATCCAG AGCTGGTCAA GTACTGGGCA 
CAACGCTACC GGCTGTTCTC TCGGTTTGAC GAGGGAATTA AACTGGACAG AGAGGGTTGG 
TTTTCTGTTA CTCCTGAGAA AATAGCTGAG CATATTGCAG TCCGTGTTAG TCAGTCATTC 
AACTGCGATA TCATAGTGGA TGCGTTCTGT GGGGTTGGAG GAAACGCTAT TCAGTTTGCA 
TTGACCTCAA AAAGAGTGAT CGCTATCGAC ATCGATCCCG AGAAGCTCAG CCTGGCGCGC 
AACAACGCAG AGGTGTACGG TGTGGCGGAT CAGATCGAGT TCGTGTGCGG GGACTTCATG 
GTGCTGGCTG CCGGCCTGCA GGCAGATGTG GTGTTCCTCA GCCCACCCTG GGGGGGACCT 
GATTATGCCA CTGCCGAAAT CTTCGATATC CAGACCATGA TTTGCCCAGA TGGATTTGAA 
ATTTTCAGGC TCTCCAAGAA GATCACCAAC AACATTGTGT ATTTTCTACC TCGGAATGCT 
GATCTTGATC AGGTGGCTTC CTTAGCAGGC CCAGGAGGGA AAGTTGAAAT AGAACAAAAT 
TTTCTCAATA ACAAACTGAA GACGATAACA GCTTATTTTG GTGATCTAAT AAGACATGAC 
ATCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017688785.1
CDS	44..2692
Translation	MVQDPGGMLVAELVLRAGAARSILCLCSRAFVEDRKLYKLGLKGFYVKDDNDSTGEEEASEEENHCPNVRFKVDTNHALDPEEVELDSEAELMKSMGLPVQFGGQSAQRDFVATENYKKRNNVKFMKKKKKKKKELQQKHEDKRGQECQDEGCGGSIQAISSELALATEQCEMSSKSQIVAEENCESSESLANEALSSELQEKWEKYWSEYGEGLLWQSWLQKHQEVSSSEAITASEPWNSLDTKGEWEQHYNELYWYYWEQFQYWTSQGWTIESSHGDKVEVNGVTQETGPLGEMNLVSQGPECSEVLSLELSPSNTRSDETLPSSAELHSEIISGICNLNLNLKEVEQSSAALTVAHQGHQEHSLSDSGSQKEPCDGGTRKRSASCENKSVNQSGSQESCSLNSNEKEQLLLHDQDEDEDEEPPEYRHVKVKRSHELDVDENPVEDPEETCSVLGFKCGTGQKYGGIPDFTHRSVQYLEKKAKLKSKYLDMRKPRKSKNTHIFFTEESEMTCKKSKTLKKVEMFLKRVSKPEEEATSQTAVPQSKSEALSESSSDSEGQEGLTATQTVMLNAENQKPPSSSAACTEPVGSNLEEEAQEAAESGSNEQGAAGPERGGGRQLVSLDIPDYLQANTEDVSQAVDEKITTKKKEKKRRRNKIALRSIPAEIAADPELVKYWAQRYRLFSRFDEGIKLDREGWFSVTPEKIAEHIAVRVSQSFNCDIIVDAFCGVGGNAIQFALTSKRVIAIDIDPEKLSLARNNAEVYGVADQIEFVCGDFMVLAAGLQADVVFLSPPWGGPDYATAEIFDIQTMICPDGFEIFRLSKKITNNIVYFLPRNADLDQVASLAGPGGKVEIEQNFLNNKLKTITAYFGDLIRHDIS

Target ORF information:

RefSeq Version	XM_017833296.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata trimethylguanosine synthase 1 (TGS1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017833296.1

ORF Insert Sequence:

ATGGTGCAGG ACCCGGGCGG GATGCTGGTG GCGGAGCTGG TGCTGCGGGC GGGGGCGGCT 
CGCAGCATCC TCTGCCTCTG CTCCCGCGCC TTCGTCGAAG ATCGAAAATT ATATAAACTG 
GGATTAAAAG GATTTTATGT CAAAGATGAC AATGACAGTA CAGGGGAAGA AGAAGCGTCT 
GAGGAGGAGA ACCATTGTCC CAATGTGAGA TTTAAGGTGG ATACTAATCA TGCGCTGGAC 
CCAGAAGAAG TTGAACTAGA CTCAGAAGCT GAGCTTATGA AGAGCATGGG GTTGCCTGTT 
CAGTTTGGTG GGCAGTCAGC CCAGAGAGAC TTTGTGGCAA CAGAAAATTA TAAAAAGAGA 
AATAATGTGA AATTTATGAA AAAGAAAAAG AAGAAGAAAA AAGAGTTACA GCAAAAACAT 
GAGGATAAAA GGGGACAGGA ATGCCAGGAT GAAGGTTGTG GTGGTAGTAT CCAGGCCATT 
TCTAGTGAGC TGGCCCTAGC TACAGAGCAA TGTGAGATGA GCAGTAAATC TCAGATTGTA 
GCTGAAGAGA ACTGTGAAAG TTCAGAAAGC CTTGCAAATG AGGCTTTATC CAGTGAACTT 
CAGGAAAAAT GGGAGAAGTA CTGGAGCGAG TATGGAGAGG GCCTTCTTTG GCAAAGTTGG 
TTGCAGAAGC ATCAAGAGGT CTCATCATCT GAAGCCATCA CAGCCTCTGA GCCTTGGAAT 
AGTCTGGACA CCAAGGGAGA ATGGGAGCAG CATTATAATG AACTGTACTG GTATTATTGG 
GAACAGTTTC AGTACTGGAC AAGTCAAGGA TGGACTATTG AGAGCTCACA TGGGGACAAG 
GTGGAAGTTA ATGGGGTTAC GCAGGAGACT GGTCCTTTGG GAGAAATGAA CTTGGTTAGC 
CAAGGGCCTG AATGCAGTGA AGTGCTGAGC TTGGAGCTGT CTCCCTCTAA CACCAGAAGT 
GATGAAACAC TCCCTTCAAG TGCTGAGCTG CACAGTGAAA TCATCTCTGG AATTTGTAAT 
TTAAATCTGA ATTTAAAGGA AGTGGAGCAG AGCAGCGCAG CTCTAACAGT AGCCCACCAA 
GGTCATCAAG AGCACAGTTT GTCTGACAGT GGAAGTCAGA AGGAGCCCTG TGATGGAGGA 
ACCAGGAAAA GGAGCGCATC TTGTGAAAAT AAAAGTGTTA ACCAGTCAGG TTCACAGGAG 
TCATGTAGCC TGAATTCAAA TGAAAAAGAG CAGTTGCTGC TTCATGACCA AGATGAAGAT 
GAGGACGAAG AGCCTCCTGA ATACAGACAT GTCAAAGTCA AGAGAAGTCA TGAACTGGAT 
GTGGATGAGA ACCCAGTGGA AGATCCTGAA GAAACCTGCT CTGTGTTGGG TTTCAAGTGT 
GGAACAGGAC AAAAGTATGG TGGAATTCCA GATTTCACCC ACAGAAGTGT GCAGTACTTG 
GAGAAAAAAG CAAAACTCAA GTCCAAATAC TTGGACATGC GTAAACCAAG GAAGAGCAAA 
AACACACACA TCTTCTTTAC AGAGGAATCT GAAATGACAT GCAAAAAAAG CAAAACTTTG 
AAGAAGGTGG AAATGTTCCT GAAGCGGGTT AGTAAACCCG AGGAGGAAGC TACATCCCAG 
ACAGCCGTCC CTCAGAGTAA ATCGGAGGCA TTGTCTGAGA GCAGCAGCGA CTCAGAGGGT 
CAGGAAGGCC TTACTGCCAC ACAGACTGTG ATGCTGAATG CTGAAAACCA AAAGCCACCA 
TCTTCCAGTG CAGCCTGCAC AGAACCTGTA GGGAGCAACC TTGAGGAGGA GGCACAGGAA 
GCTGCAGAGA GTGGCTCCAA TGAGCAGGGT GCAGCGGGGC CAGAGCGTGG TGGTGGGCGG 
CAGCTTGTCT CTCTGGATAT TCCCGATTAC CTCCAGGCAA ACACAGAGGA CGTCAGCCAA 
GCTGTAGATG AAAAAATAAC CACAAAGAAG AAGGAGAAGA AAAGAAGAAG GAATAAAATT 
GCACTGAGAT CTATACCTGC TGAGATTGCT GCTGATCCAG AGCTGGTCAA GTACTGGGCA 
CAACGCTACC GGCTGTTCTC TCGGTTTGAC GAGGGAATTA AACTGGACAG AGAGGGTTGG 
TTTTCTGTTA CTCCTGAGAA AATAGCTGAG CATATTGCAG TCCGTGTTAG TCAGTCATTC 
AACTGCGATA TCATAGTGGA TGCGTTCTGT GGGGTTGGAG GAAACGCTAT TCAGTTTGCA 
TTGACCTCAA AAAGAGTGAT CGCTATCGAC ATCGATCCCG AGAAGCTCAG CCTGGCGCGC 
AACAACGCAG AGGTGTACGG TGTGGCGGAT CAGATCGAGT TCGTGTGCGG GGACTTCATG 
GTGCTGGCTG CCGGCCTGCA GGCAGATGTG GTGTTCCTCA GCCCACCCTG GGGGGGACCT 
GATTATGCCA CTGCCGAAAT CTTCGATATC CAGACCATGA TTTGCCCAGA TGGATTTGAA 
ATTTTCAGGC TCTCCAAGAA GATCACCAAC AACATTGTGT ATTTTCTACC TCGGAATGCT 
GATCTTGATC AGGTGGCTTC CTTAGCAGGC CCAGGAGGGA AAGTTGAAAT AGAACAAAAT 
TTTCTCAATA ACAAACTGAA GACGATAACA GCTTATTTTG GTGATCTAAT AAGACATGAC 
ATCTCCTGA