HIF1A cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIF1A
Entrez Gene ID	108493788
Full Name	hypoxia inducible factor 1 alpha subunit
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017807587.1	XP_017663076.1	hypoxia-inducible factor 1-alpha

Pathways from BioSystems
Gene Ontology

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLe53212	XM_017807587.1 Latest version!	Lepidothrix coronata hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIF1A ( XM_017807587.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017663076.1 Lepidothrix coronata hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X1, mRNA.

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CloneID	OLe53212
Clone ID Related Accession (Same CDS sequence)	XM_017807587.1
Accession Version	XM_017807587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	hypoxia-inducible factor 1-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690199.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CCGGCGGTGT CACCGACAAG AAGAGGATTA GCTCTGAACG CAGAAAAGAG 
AAGTCAAGGG ATGCGGCCCG GTGCCGAAGG AGCAAGGAGT CCGAAGTGTT CTATGAACTC 
GCCCACCAGC TGCCTCTGCC CCACACCGTG AGCGCACACC TGGACAAGGC GTCCATCATG 
AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTGGAGACA 
GAAGCCAAAA TGGAGAAGGA ACTGAACTGT TTCTACTTGA AGGCTCTTGA TGGATTTGTC 
ATGGTTCTGT CCGAAGATGG GGACATGATT TACATGTCTG AAAATGTGAA CAAGTGCATG 
GGACTCACTC AGTTTGAGTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC 
CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG 
CAAAACACGG AGCGCAGCTT TTTTCTCAGA ATGAAGTGTA CACTGACTAG CAGGGGAAGA 
ACAGTGAATA TAAAGTCTGC TACCTGGAAG GTGCTGCACT GCACTGGACA CATCCGTGTG 
TACGACACGT GTGGTAACCA AACTCACTGC GGCTACAAAA AGCCTCCCAT GACGTGTCTG 
GTGTTGATCT GTGAACCTAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA 
ACGTTCCTCA GTCGTCACAG TCTTGACATG AAATTTTCCT ATTGTGATGA AAGAATTACA 
GAGTTGATGG GGTATGAGCC AGAGGAGCTC CTGGGCCGCT CAATCTATGA GTACTATCAC 
GCACTGGATT CTGATCATCT GACCAAAACA CACCATGACA TGTTCACAAA AGGGCAGGTG 
ACAACAGGGC AGTACAGAAT GCTGGCAAAA CAAGGTGGCT ATGTCTGGGT TGAAACTCAA 
GCAACTGTTA TATACAACAC TAAGAATTCT CAGCCACAGT GCATAGTGTG TGTAAACTAT 
GTGTTGAGTG GAATTGTTCA GAAGGACTTA ATTTTTTCCC TTGGACAAAC TGAGTGTATG 
CTGAAACCAG TGGAGTCTCC TGATATGAAA ATGACCAAAA TATTCAGTAA AGATGACCTG 
GATGATACCA ACAGCCTCTT TGAAAAACTT AAACAGGAAC CAGATGCTTT AACTGTGCTG 
GCCCCAGCTG CTGGAGACAC AATTATCTCT CTAGATTTCA GCAGTAATGA GTCTGATGAA 
CAACAATGTG ATGAAGTTCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA 
TTGCAGAATA TAAATATGGC AATGTCCCCA CTACCTGCCT CTGAAACTAC AAAGCCACTT 
CGTAGCAATG CTGATCCTGC ACTCAATAGA GAAGTTGTAT TAAAGCTGGA GCCAAACACA 
GAGCCACTCG AACTTTCTTT TACCATGCCT CAGGTGCAAG AGCAACCAAC CAGCCCTTCT 
GATGCAAGTA CCAGCCAAAG TTCACCTGAG CCCAGTAGTC CCAACGACTA CTGCTTTGAC 
GTGGATAATG ATATGGCGAA TGAATTCAAA CTGGAATTAG TGGAGAAACT CTTTGCCATA 
GATACAGAAG CAAAAAATCC GTTCTCTACT CAGGAAACCG ATTTAGATTT GGAGATGTTG 
GCTCCTTACA TCCCGATGGA TGATGACTTC CAGCTGCGAT CCTTTGATCA GCTCTCTCCG 
CTGGAAAGCA GCTCTTCCAG CTCTCAGAGT GCAGCCACCA TTACCATATT TCAGCAGACT 
CAGACGCCGT CAAGTCCTGC TGAGGAGATA AAGCCGGCGG CGGAACGTGC GGACGATGTG 
AAGACAATAA TCGTCCCCTC GTCTCCTGTG CACGTGGGCA GCGAAACGAG TAGTGCCCCG 
GCGTCGCCTT ACGGTGGGAA CAGGAGTCGA ACCGCCTCTC CAATCAGAGC AGGAAAAGGA 
ACAGTGGATC AGACGGAAAA ACCTTCTCCA GGAGCACCCA GCTTGCTAAC AGTAACTCTG 
AATAAAAGAT CTACTGCACT GGATGAAGAA CTAAATCCAA AGATGCTAGC TTTGCATAAT 
GCTCAGAGAA AACGAAAAAT GGAACATGAT GGTTCACTTT TTCAGGCAGT TGGAATTGGG 
TCTTTATTCC AGCAGACAGG AGACCGTGGA GGAAATGCAT CACTTGCTTG GAAACGTGTA 
AAGGGATGCA AAACAAATGG TCATAATGGA GTGGAGCAAA AGACAATCAT TCTACTATCA 
ACTGACATAG CAAGTAAACT TCTAGGGCAG TCGATGGATG AGAGTGGACT CCCCCAACTC 
ACGAGTTACG ACTGCGAAGT AAACGCTCCC ATACAAGGCA ACAGAAACCT GCTACAGGGT 
GAAGAACTGC TCAGAGCTCT GGATCAAGTT AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017663076.1
CDS	103..2538
Translation	MDSPGGVTDKKRISSERRKEKSRDAARCRRSKESEVFYELAHQLPLPHTVSAHLDKASIMRLTISYLRMRKLLDAGELETEAKMEKELNCFYLKALDGFVMVLSEDGDMIYMSENVNKCMGLTQFELTGHSVFDFTHPCDHEELREMLTHRNGPVKKGKEQNTERSFFLRMKCTLTSRGRTVNIKSATWKVLHCTGHIRVYDTCGNQTHCGYKKPPMTCLVLICEPIPHPSNIEVPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKQGGYVWVETQATVIYNTKNSQPQCIVCVNYVLSGIVQKDLIFSLGQTECMLKPVESPDMKMTKIFSKDDLDDTNSLFEKLKQEPDALTVLAPAAGDTIISLDFSSNESDEQQCDEVPLYNDVMLPSSSEKLQNINMAMSPLPASETTKPLRSNADPALNREVVLKLEPNTEPLELSFTMPQVQEQPTSPSDASTSQSSPEPSSPNDYCFDVDNDMANEFKLELVEKLFAIDTEAKNPFSTQETDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSSSSQSAATITIFQQTQTPSSPAEEIKPAAERADDVKTIIVPSSPVHVGSETSSAPASPYGGNRSRTASPIRAGKGTVDQTEKPSPGAPSLLTVTLNKRSTALDEELNPKMLALHNAQRKRKMEHDGSLFQAVGIGSLFQQTGDRGGNASLAWKRVKGCKTNGHNGVEQKTIILLSTDIASKLLGQSMDESGLPQLTSYDCEVNAPIQGNRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_017807587.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807587.1

ORF Insert Sequence:

ATGGACAGCC CCGGCGGTGT CACCGACAAG AAGAGGATTA GCTCTGAACG CAGAAAAGAG 
AAGTCAAGGG ATGCGGCCCG GTGCCGAAGG AGCAAGGAGT CCGAAGTGTT CTATGAACTC 
GCCCACCAGC TGCCTCTGCC CCACACCGTG AGCGCACACC TGGACAAGGC GTCCATCATG 
AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTGGAGACA 
GAAGCCAAAA TGGAGAAGGA ACTGAACTGT TTCTACTTGA AGGCTCTTGA TGGATTTGTC 
ATGGTTCTGT CCGAAGATGG GGACATGATT TACATGTCTG AAAATGTGAA CAAGTGCATG 
GGACTCACTC AGTTTGAGTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC 
CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG 
CAAAACACGG AGCGCAGCTT TTTTCTCAGA ATGAAGTGTA CACTGACTAG CAGGGGAAGA 
ACAGTGAATA TAAAGTCTGC TACCTGGAAG GTGCTGCACT GCACTGGACA CATCCGTGTG 
TACGACACGT GTGGTAACCA AACTCACTGC GGCTACAAAA AGCCTCCCAT GACGTGTCTG 
GTGTTGATCT GTGAACCTAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA 
ACGTTCCTCA GTCGTCACAG TCTTGACATG AAATTTTCCT ATTGTGATGA AAGAATTACA 
GAGTTGATGG GGTATGAGCC AGAGGAGCTC CTGGGCCGCT CAATCTATGA GTACTATCAC 
GCACTGGATT CTGATCATCT GACCAAAACA CACCATGACA TGTTCACAAA AGGGCAGGTG 
ACAACAGGGC AGTACAGAAT GCTGGCAAAA CAAGGTGGCT ATGTCTGGGT TGAAACTCAA 
GCAACTGTTA TATACAACAC TAAGAATTCT CAGCCACAGT GCATAGTGTG TGTAAACTAT 
GTGTTGAGTG GAATTGTTCA GAAGGACTTA ATTTTTTCCC TTGGACAAAC TGAGTGTATG 
CTGAAACCAG TGGAGTCTCC TGATATGAAA ATGACCAAAA TATTCAGTAA AGATGACCTG 
GATGATACCA ACAGCCTCTT TGAAAAACTT AAACAGGAAC CAGATGCTTT AACTGTGCTG 
GCCCCAGCTG CTGGAGACAC AATTATCTCT CTAGATTTCA GCAGTAATGA GTCTGATGAA 
CAACAATGTG ATGAAGTTCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA 
TTGCAGAATA TAAATATGGC AATGTCCCCA CTACCTGCCT CTGAAACTAC AAAGCCACTT 
CGTAGCAATG CTGATCCTGC ACTCAATAGA GAAGTTGTAT TAAAGCTGGA GCCAAACACA 
GAGCCACTCG AACTTTCTTT TACCATGCCT CAGGTGCAAG AGCAACCAAC CAGCCCTTCT 
GATGCAAGTA CCAGCCAAAG TTCACCTGAG CCCAGTAGTC CCAACGACTA CTGCTTTGAC 
GTGGATAATG ATATGGCGAA TGAATTCAAA CTGGAATTAG TGGAGAAACT CTTTGCCATA 
GATACAGAAG CAAAAAATCC GTTCTCTACT CAGGAAACCG ATTTAGATTT GGAGATGTTG 
GCTCCTTACA TCCCGATGGA TGATGACTTC CAGCTGCGAT CCTTTGATCA GCTCTCTCCG 
CTGGAAAGCA GCTCTTCCAG CTCTCAGAGT GCAGCCACCA TTACCATATT TCAGCAGACT 
CAGACGCCGT CAAGTCCTGC TGAGGAGATA AAGCCGGCGG CGGAACGTGC GGACGATGTG 
AAGACAATAA TCGTCCCCTC GTCTCCTGTG CACGTGGGCA GCGAAACGAG TAGTGCCCCG 
GCGTCGCCTT ACGGTGGGAA CAGGAGTCGA ACCGCCTCTC CAATCAGAGC AGGAAAAGGA 
ACAGTGGATC AGACGGAAAA ACCTTCTCCA GGAGCACCCA GCTTGCTAAC AGTAACTCTG 
AATAAAAGAT CTACTGCACT GGATGAAGAA CTAAATCCAA AGATGCTAGC TTTGCATAAT 
GCTCAGAGAA AACGAAAAAT GGAACATGAT GGTTCACTTT TTCAGGCAGT TGGAATTGGG 
TCTTTATTCC AGCAGACAGG AGACCGTGGA GGAAATGCAT CACTTGCTTG GAAACGTGTA 
AAGGGATGCA AAACAAATGG TCATAATGGA GTGGAGCAAA AGACAATCAT TCTACTATCA 
ACTGACATAG CAAGTAAACT TCTAGGGCAG TCGATGGATG AGAGTGGACT CCCCCAACTC 
ACGAGTTACG ACTGCGAAGT AAACGCTCCC ATACAAGGCA ACAGAAACCT GCTACAGGGT 
GAAGAACTGC TCAGAGCTCT GGATCAAGTT AACTGA