KCNMA1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	KCNMA1
Entrez Gene ID	108493517
Full Name	potassium calcium-activated channel subfamily M alpha 1
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017807032.1	XP_017662521.1	calcium-activated potassium channel subunit alpha-1 isoform X14
XM_017807034.1	XP_017662523.1	calcium-activated potassium channel subunit alpha-1 isoform X16
XM_017807035.1	XP_017662524.1	calcium-activated potassium channel subunit alpha-1 isoform X17
XM_017807036.1	XP_017662525.1	calcium-activated potassium channel subunit alpha-1 isoform X18
XM_017807050.1	XP_017662539.1	calcium-activated potassium channel subunit alpha-1 isoform X32
XM_017807057.1	XP_017662546.1	calcium-activated potassium channel subunit alpha-1 isoform X39
XM_017807023.1	XP_017662512.1	calcium-activated potassium channel subunit alpha-1 isoform X6
XM_017807025.1	XP_017662514.1	calcium-activated potassium channel subunit alpha-1 isoform X8
XM_017807029.1	XP_017662518.1	calcium-activated potassium channel subunit alpha-1 isoform X12
XM_017807030.1	XP_017662519.1	calcium-activated potassium channel subunit alpha-1 isoform X13
XM_017807033.1	XP_017662522.1	calcium-activated potassium channel subunit alpha-1 isoform X15
XM_017807039.1	XP_017662528.1	calcium-activated potassium channel subunit alpha-1 isoform X21
XM_017807046.1	XP_017662535.1	calcium-activated potassium channel subunit alpha-1 isoform X28
XM_017807058.1	XP_017662547.1	calcium-activated potassium channel subunit alpha-1 isoform X40
XM_017807059.1	XP_017662548.1	calcium-activated potassium channel subunit alpha-1 isoform X41
XM_017807019.1	XP_017662508.1	calcium-activated potassium channel subunit alpha-1 isoform X2
XM_017807028.1	XP_017662517.1	calcium-activated potassium channel subunit alpha-1 isoform X11
XM_017807041.1	XP_017662530.1	calcium-activated potassium channel subunit alpha-1 isoform X23
XM_017807045.1	XP_017662534.1	calcium-activated potassium channel subunit alpha-1 isoform X27
XM_017807051.1	XP_017662540.1	calcium-activated potassium channel subunit alpha-1 isoform X33
XM_017807055.1	XP_017662544.1	calcium-activated potassium channel subunit alpha-1 isoform X37
XM_017807020.1	XP_017662509.1	calcium-activated potassium channel subunit alpha-1 isoform X3
XM_017807022.1	XP_017662511.1	calcium-activated potassium channel subunit alpha-1 isoform X5
XM_017807018.1	XP_017662507.1	calcium-activated potassium channel subunit alpha-1 isoform X1
XM_017807021.1	XP_017662510.1	calcium-activated potassium channel subunit alpha-1 isoform X4
XM_017807024.1	XP_017662513.1	calcium-activated potassium channel subunit alpha-1 isoform X7
XM_017807027.1	XP_017662516.1	calcium-activated potassium channel subunit alpha-1 isoform X10
XM_017807037.1	XP_017662526.1	calcium-activated potassium channel subunit alpha-1 isoform X19
XM_017807040.1	XP_017662529.1	calcium-activated potassium channel subunit alpha-1 isoform X22
XM_017807042.1	XP_017662531.1	calcium-activated potassium channel subunit alpha-1 isoform X24
XM_017807043.1	XP_017662532.1	calcium-activated potassium channel subunit alpha-1 isoform X25
XM_017807044.1	XP_017662533.1	calcium-activated potassium channel subunit alpha-1 isoform X26
XM_017807047.1	XP_017662536.1	calcium-activated potassium channel subunit alpha-1 isoform X29
XM_017807048.1	XP_017662537.1	calcium-activated potassium channel subunit alpha-1 isoform X30
XM_017807049.1	XP_017662538.1	calcium-activated potassium channel subunit alpha-1 isoform X31
XM_017807053.1	XP_017662542.1	calcium-activated potassium channel subunit alpha-1 isoform X35
XM_017807056.1	XP_017662545.1	calcium-activated potassium channel subunit alpha-1 isoform X38
XM_017807026.1	XP_017662515.1	calcium-activated potassium channel subunit alpha-1 isoform X9
XM_017807038.1	XP_017662527.1	calcium-activated potassium channel subunit alpha-1 isoform X20
XM_017807052.1	XP_017662541.1	calcium-activated potassium channel subunit alpha-1 isoform X34
XM_017807054.1	XP_017662543.1	calcium-activated potassium channel subunit alpha-1 isoform X36

Pathways from BioSystems
Gene Ontology

The following KCNMA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNMA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLe52792	XM_017807018.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52793	XM_017807019.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52794	XM_017807020.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52795	XM_017807021.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52796	XM_017807022.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52797	XM_017807023.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52798	XM_017807024.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52799	XM_017807025.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52800	XM_017807026.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52801	XM_017807027.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52802	XM_017807028.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52803	XM_017807029.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52804	XM_017807030.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52805	XM_017807032.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52806	XM_017807033.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52807	XM_017807034.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52808	XM_017807035.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52809	XM_017807036.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52810	XM_017807037.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52811	XM_017807038.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52812	XM_017807039.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52813	XM_017807040.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52814	XM_017807041.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52815	XM_017807042.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52816	XM_017807043.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52817	XM_017807044.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52818	XM_017807045.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52819	XM_017807046.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X28, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52820	XM_017807047.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X29, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52821	XM_017807048.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52822	XM_017807049.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52823	XM_017807050.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52824	XM_017807051.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X33, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLe52825	XM_017807052.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLe52826	XM_017807053.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X35, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLe52827	XM_017807054.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X36, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLe52828	XM_017807055.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X37, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLe52829	XM_017807056.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X38, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLe52830	XM_017807057.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X39, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLe52831	XM_017807058.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X40, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLe52832	XM_017807059.1 Latest version!	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X41, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KCNMA1 ( XM_017807018.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662507.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X1, mRNA.

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CloneID	OLe52792
Clone ID Related Accession (Same CDS sequence)	XM_017807018.1
Accession Version	XM_017807018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGTATATTAA TCAATCCTGG AAACCACGTC 
AAGATTCAAG AAGGTACCTT AGGATTCTTC ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG 
GCATATTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA 
TGTGGCTGCA AACGGCAAGT AAAGAATGCA GCTAGATCAA GTTACCCAAA ATGCTCATAT 
CAATTCAAGA ATGCAACTAC TAATGCATTG GTTGATGCCA AGTCCACTGA AGGAATCCTG 
GAACCTGTTC CACTTGTTAA TAACCGTAAA GGGAGCCTCT TCCTACCCAA CAACCCCTCG 
CTGCTGCACC TTAACTTACT GAGTTCTGTC GAACAAGGAA AATCTACGTA CTGTAGATTG 
CAAAGGGCAC TCAGCTTACC TGTAAAATAC CGCTGCCACT CCCAAAAGCC TGGGTTGAAC 
CAAGATCTCC TTGAAGATGA GCAGCCATCG ACACTATCAC CCAAAAAAAA GCAGCGAAAC 
GGAGGCATGA GAAATTCACC CAACTCCTCA CCCAAGCTGA TGAGGCATGA CCCCTTGTTA 
ATTCCTGGCA ATGAGCAGAT TGACAATATG GATGCCAACG TGAAAAAATA TGACTCTACA 
GGGATGTTTC ATTGGTGTCC AGCCAAGGAC ATTGAAAAGG TCATTTTGAC TCGAAGTGAA 
GCAGCGATGA CAGTTTTAAG TGGGCATGTA GTCGTTTGCA TATTTGGGGA TGTTAAATCT 
GCTTTGATTG GATTAAGAAA TTTAGTGATG CCATTACGAG CCAGCAACTT TCACTACCAT 
GAACTCAAGC ACATTGTGTT TGTGGGTTCA CTTGAATACC TGAGAAGGGA ATGGGAGACA 
CTGCATAACT TCCCCAAGGT TTCAATCTTG CCTGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGCGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATG CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT TGGGGTCTTG CAGGCTAATT CCCAAGGCTT TACACCCCCT 
GGAATGGATA GGTCATCTCC AGACAACAGT CCAGTCCATG GCCTGTTACG TCAACCCTCC 
ATTACAACAG GAGCCAACAT CCCTATTATT ACAGAGCTAG TGAATGATTC AAATGTTCAG 
TTCTTGGACC AAGATGATGA TGATGATCCA GACACAGAAC TGTATCTCAC ACAGCCCTTT 
GCATGTGGAA CCGCGTTTGC TGTCAGTGTG CTGGACTCTC TCATGAGCGC AACATACTTC 
AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG 
GAAGCACTGA TTGCTGAGGA AAATGCATTG AGAGGAGGTT ACAGCACGCC CCAGACACTT 
GCAAACAGGG ACAGGTGTCG AGTTGCTCAG CTGGCTTTGT ATGATGGGCC GTTTGCAGAC 
CTAGGGGATG GAGGTTGTTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG 
CTTTGCTTTG GAATTTATCG ATTAAGGGAT GCACATCTAA GCACTCCCAG CCAATGCACT 
AAACGTTATG TTATCACAAA CCCACCGTAT GAATTTGAGC TTGTGCCGAC AGACTTGATC 
TTCTGTTTGA TGCAATTTGA CCACAATGCT GGCCAGTCCC GAGCCAGTCT TTCTCATTCC 
TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCTAT CCCATCAACA 
GCAAATCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG 
ATGAATAGAA TGGGCCAAGC AGAAAAGAAA TGGTTTACAG ATGAGCCGGA TAATGCCTAT 
CCCAGAAACA TTCAAATCAA GCCCATGAGC ACTCACATGG CCAATCAGAT CAATCAATAT 
AAATCAACAA GCAGCTTGAT ACCACCAATC CGAGAAGTTG AAGATGAATG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662507.1
CDS	355..4308
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRQVKNAARSSYPKCSYQFKNATTNALVDAKSTEGILEPVPLVNNRKGSLFLPNNPSLLHLNLLSSVEQGKSTYCRLQRALSLPVKYRCHSQKPGLNQDLLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807018.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807018.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGTATATTAA TCAATCCTGG AAACCACGTC 
AAGATTCAAG AAGGTACCTT AGGATTCTTC ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG 
GCATATTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA 
TGTGGCTGCA AACGGCAAGT AAAGAATGCA GCTAGATCAA GTTACCCAAA ATGCTCATAT 
CAATTCAAGA ATGCAACTAC TAATGCATTG GTTGATGCCA AGTCCACTGA AGGAATCCTG 
GAACCTGTTC CACTTGTTAA TAACCGTAAA GGGAGCCTCT TCCTACCCAA CAACCCCTCG 
CTGCTGCACC TTAACTTACT GAGTTCTGTC GAACAAGGAA AATCTACGTA CTGTAGATTG 
CAAAGGGCAC TCAGCTTACC TGTAAAATAC CGCTGCCACT CCCAAAAGCC TGGGTTGAAC 
CAAGATCTCC TTGAAGATGA GCAGCCATCG ACACTATCAC CCAAAAAAAA GCAGCGAAAC 
GGAGGCATGA GAAATTCACC CAACTCCTCA CCCAAGCTGA TGAGGCATGA CCCCTTGTTA 
ATTCCTGGCA ATGAGCAGAT TGACAATATG GATGCCAACG TGAAAAAATA TGACTCTACA 
GGGATGTTTC ATTGGTGTCC AGCCAAGGAC ATTGAAAAGG TCATTTTGAC TCGAAGTGAA 
GCAGCGATGA CAGTTTTAAG TGGGCATGTA GTCGTTTGCA TATTTGGGGA TGTTAAATCT 
GCTTTGATTG GATTAAGAAA TTTAGTGATG CCATTACGAG CCAGCAACTT TCACTACCAT 
GAACTCAAGC ACATTGTGTT TGTGGGTTCA CTTGAATACC TGAGAAGGGA ATGGGAGACA 
CTGCATAACT TCCCCAAGGT TTCAATCTTG CCTGGTACGC CATTAAGTCG GGCTGATTTA 
AGGGCTGTCA ACATCAACCT CTGCGACATG TGCGTTATCC TGTCAGCCAA TCAGAATAAT 
ATTGATGATG CTTCGCTGCA GGACAAGGAA TGCATCTTGG CGTCACTCAA CATCAAATCT 
ATGCAGTTTG ATGACAGCAT TGGGGTCTTG CAGGCTAATT CCCAAGGCTT TACACCCCCT 
GGAATGGATA GGTCATCTCC AGACAACAGT CCAGTCCATG GCCTGTTACG TCAACCCTCC 
ATTACAACAG GAGCCAACAT CCCTATTATT ACAGAGCTAG TGAATGATTC AAATGTTCAG 
TTCTTGGACC AAGATGATGA TGATGATCCA GACACAGAAC TGTATCTCAC ACAGCCCTTT 
GCATGTGGAA CCGCGTTTGC TGTCAGTGTG CTGGACTCTC TCATGAGCGC AACATACTTC 
AATGACAATA TCCTCACACT GATACGGACA TTGGTAACAG GAGGAGCCAC ACCAGAGCTG 
GAAGCACTGA TTGCTGAGGA AAATGCATTG AGAGGAGGTT ACAGCACGCC CCAGACACTT 
GCAAACAGGG ACAGGTGTCG AGTTGCTCAG CTGGCTTTGT ATGATGGGCC GTTTGCAGAC 
CTAGGGGATG GAGGTTGTTA TGGAGATCTA TTTTGTAAAG CACTGAAAAC ATACAATATG 
CTTTGCTTTG GAATTTATCG ATTAAGGGAT GCACATCTAA GCACTCCCAG CCAATGCACT 
AAACGTTATG TTATCACAAA CCCACCGTAT GAATTTGAGC TTGTGCCGAC AGACTTGATC 
TTCTGTTTGA TGCAATTTGA CCACAATGCT GGCCAGTCCC GAGCCAGTCT TTCTCATTCC 
TCCCATTCCT CCTATTCTTC CAGCAAGAAG AGCTCATCAG TTCACTCTAT CCCATCAACA 
GCAAATCGAC CGAACCGCAC CAAGACCAGG GATTCCCGGG AAAAACAGAA TGCAACAAGG 
ATGAATAGAA TGGGCCAAGC AGAAAAGAAA TGGTTTACAG ATGAGCCGGA TAATGCCTAT 
CCCAGAAACA TTCAAATCAA GCCCATGAGC ACTCACATGG CCAATCAGAT CAATCAATAT 
AAATCAACAA GCAGCTTGAT ACCACCAATC CGAGAAGTTG AAGATGAATG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807019.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662508.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X2, mRNA.

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CloneID	OLe52793
Clone ID Related Accession (Same CDS sequence)	XM_017807019.1
Accession Version	XM_017807019.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3771bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCAAGTAAAG 
AATGCAGCTA GATCAAGTTA CCCAAAATGC TCATATCAAT TCAAGAATGC AACTACTAAT 
GCATTGGTTG ATGCCAAGTC CACTGAAGGA ATCCTGGAAC CTGTTCCACT TGTTAATAAC 
CGTAAAGGGA GCCTCTTCCT ACCCAACAAC CCCTCGCTGC TGCACCTTAA CTTACTGAGT 
TCTGTCGAAC AAGGAAAATC TACGTACTGT AGATTGCAAA GGGCACTCAG CTTACCTGTA 
AAATACCGCT GCCACTCCCA AAAGCCTGGG TTGAACCAAG ATCTCCTTGA AGATGAGCAG 
CCATCGACAC TATCACCCAA AAAAAAGCAG CGAAACGGAG GCATGAGAAA TTCACCCAAC 
TCCTCACCCA AGCTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA GCAGATTGAC 
AATATGGATG CCAACGTGAA AAAATATGAC TCTACAGGGA TGTTTCATTG GTGTCCAGCC 
AAGGACATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG 
CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA 
GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG 
GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACACTGC ATAACTTCCC CAAGGTTTCA 
ATCTTGCCTG GTACGCCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC 
GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC 
AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG 
GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGAA TGGATAGGTC ATCTCCAGAC 
AACAGTCCAG TCCATGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT 
ATTATTACAG AGCTAGTGAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT 
GATCCAGACA CAGAACTGTA TCTCACACAG CCCTTTGCAT GTGGAACCGC GTTTGCTGTC 
AGTGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA 
CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC TGAGGAAAAT 
GCATTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT 
GCTCAGCTGG CTTTGTATGA TGGGCCGTTT GCAGACCTAG GGGATGGAGG TTGTTATGGA 
GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA 
AGGGATGCAC ATCTAAGCAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA 
CCGTATGAAT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTTGACCAC 
AATGCTGGCC AGTCCCGAGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC 
AAGAAGAGCT CATCAGTTCA CTCTATCCCA TCAACAGCAA ATCGACCGAA CCGCACCAAG 
ACCAGGGATT CCCGGGAAAA ACAGAAGTAT GTGCAGGAAG ATAGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662508.1
CDS	355..4125
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRQVKNAARSSYPKCSYQFKNATTNALVDAKSTEGILEPVPLVNNRKGSLFLPNNPSLLHLNLLSSVEQGKSTYCRLQRALSLPVKYRCHSQKPGLNQDLLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKYVQEDRL

Target ORF information:

RefSeq Version	XM_017807019.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807019.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCAAGTAAAG 
AATGCAGCTA GATCAAGTTA CCCAAAATGC TCATATCAAT TCAAGAATGC AACTACTAAT 
GCATTGGTTG ATGCCAAGTC CACTGAAGGA ATCCTGGAAC CTGTTCCACT TGTTAATAAC 
CGTAAAGGGA GCCTCTTCCT ACCCAACAAC CCCTCGCTGC TGCACCTTAA CTTACTGAGT 
TCTGTCGAAC AAGGAAAATC TACGTACTGT AGATTGCAAA GGGCACTCAG CTTACCTGTA 
AAATACCGCT GCCACTCCCA AAAGCCTGGG TTGAACCAAG ATCTCCTTGA AGATGAGCAG 
CCATCGACAC TATCACCCAA AAAAAAGCAG CGAAACGGAG GCATGAGAAA TTCACCCAAC 
TCCTCACCCA AGCTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA GCAGATTGAC 
AATATGGATG CCAACGTGAA AAAATATGAC TCTACAGGGA TGTTTCATTG GTGTCCAGCC 
AAGGACATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG 
CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA 
GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG 
GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACACTGC ATAACTTCCC CAAGGTTTCA 
ATCTTGCCTG GTACGCCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC 
GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC 
AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG 
GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGAA TGGATAGGTC ATCTCCAGAC 
AACAGTCCAG TCCATGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT 
ATTATTACAG AGCTAGTGAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT 
GATCCAGACA CAGAACTGTA TCTCACACAG CCCTTTGCAT GTGGAACCGC GTTTGCTGTC 
AGTGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA 
CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC TGAGGAAAAT 
GCATTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT 
GCTCAGCTGG CTTTGTATGA TGGGCCGTTT GCAGACCTAG GGGATGGAGG TTGTTATGGA 
GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA 
AGGGATGCAC ATCTAAGCAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA 
CCGTATGAAT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTTGACCAC 
AATGCTGGCC AGTCCCGAGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC 
AAGAAGAGCT CATCAGTTCA CTCTATCCCA TCAACAGCAA ATCGACCGAA CCGCACCAAG 
ACCAGGGATT CCCGGGAAAA ACAGAAGTAT GTGCAGGAAG ATAGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807020.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662509.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OLe52794
Clone ID Related Accession (Same CDS sequence)	XM_017807020.1
Accession Version	XM_017807020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT TTGGTTTCAA TCAGTCAGGA AAAAAACAGT 
GGAACCCACA TTCTTATGAT AACTGAACTG GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAGCCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG CACTCACATG GCCAATCAGA TCAATCAATA TAAATCAACA 
AGCAGCTTGA TACCACCAAT CCGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662509.1
CDS	355..4119
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807020.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807020.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT TTGGTTTCAA TCAGTCAGGA AAAAAACAGT 
GGAACCCACA TTCTTATGAT AACTGAACTG GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAGCCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG CACTCACATG GCCAATCAGA TCAATCAATA TAAATCAACA 
AGCAGCTTGA TACCACCAAT CCGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807021.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662510.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OLe52795
Clone ID Related Accession (Same CDS sequence)	XM_017807021.1
Accession Version	XM_017807021.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT TTGGTTTCAA TCAGTCAGGA AAAAAACAGT 
GGAACCCACA TTCTTATGAT AACTGAACTG GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAGCCGGATA ATGCCTATCC CAGAAACATT 
CAAATCAAGC CCATGAGCAC TCACATGGCC AATCAGATCA ATCAATATAA ATCAACAAGC 
AGCTTGATAC CACCAATCCG AGAAGTTGAA GATGAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662510.1
CDS	355..4116
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807021.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807021.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT TTGGTTTCAA TCAGTCAGGA AAAAAACAGT 
GGAACCCACA TTCTTATGAT AACTGAACTG GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAGCCGGATA ATGCCTATCC CAGAAACATT 
CAAATCAAGC CCATGAGCAC TCACATGGCC AATCAGATCA ATCAATATAA ATCAACAAGC 
AGCTTGATAC CACCAATCCG AGAAGTTGAA GATGAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807022.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662511.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X5, mRNA.

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CloneID	OLe52796
Clone ID Related Accession (Same CDS sequence)	XM_017807022.1
Accession Version	XM_017807022.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTT GAAGATGAGC AGCCATCGAC ACTATCACCC 
AAAAAAAAGC AGCGAAACGG AGGCATGAGA AATTCACCCA ACTCCTCACC CAAGCTGATG 
AGGCATGACC CCTTGTTAAT TCCTGGCAAT GAGCAGATTG ACAATATGGA TGCCAACGTG 
AAAAAATATG ACTCTACAGG GATGTTTCAT TGGTGTCCAG CCAAGGACAT TGAAAAGGTC 
ATTTTGACTC GAAGTGAAGC AGCGATGACA GTTTTAAGTG GGCATGTAGT CGTTTGCATA 
TTTGGGGATG TTAAATCTGC TTTGATTGGA TTAAGAAATT TAGTGATGCC ATTACGAGCC 
AGCAACTTTC ACTACCATGA ACTCAAGCAC ATTGTGTTTG TGGGTTCACT TGAATACCTG 
AGAAGGGAAT GGGAGACACT GCATAACTTC CCCAAGGTTT CAATCTTGCC TGGTACGCCA 
TTAAGTCGGG CTGATTTAAG GGCTGTCAAC ATCAACCTCT GCGACATGTG CGTTATCCTG 
TCAGCCAATC AGAATAATAT TGATGATGCT TCGCTGCAGG ACAAGGAATG CATCTTGGCG 
TCACTCAACA TCAAATCTAT GCAGTTTGAT GACAGCATTG GGGTCTTGCA GGCTAATTCC 
CAAGGCTTTA CACCCCCTGG AATGGATAGG TCATCTCCAG ACAACAGTCC AGTCCATGGC 
CTGTTACGTC AACCCTCCAT TACAACAGGA GCCAACATCC CTATTATTAC AGAGCTAGCT 
AAGCCTGGCA AACTTCTGCC TTTGGTTTCA ATCAGTCAGG AAAAAAACAG TGGAACCCAC 
ATTCTTATGA TAACTGAACT GGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
GCAGAAAAGA AATGGTTTAC AGATGAGCCG GATAATGCCT ATCCCAGAAA CATTCAAATC 
AAGCCCATGA GCACTCACAT GGCCAATCAG ATCAATCAAT ATAAATCAAC AAGCAGCTTG 
ATACCACCAA TCCGAGAAGT TGAAGATGAA TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662511.1
CDS	355..4110
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807022.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807022.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTT GAAGATGAGC AGCCATCGAC ACTATCACCC 
AAAAAAAAGC AGCGAAACGG AGGCATGAGA AATTCACCCA ACTCCTCACC CAAGCTGATG 
AGGCATGACC CCTTGTTAAT TCCTGGCAAT GAGCAGATTG ACAATATGGA TGCCAACGTG 
AAAAAATATG ACTCTACAGG GATGTTTCAT TGGTGTCCAG CCAAGGACAT TGAAAAGGTC 
ATTTTGACTC GAAGTGAAGC AGCGATGACA GTTTTAAGTG GGCATGTAGT CGTTTGCATA 
TTTGGGGATG TTAAATCTGC TTTGATTGGA TTAAGAAATT TAGTGATGCC ATTACGAGCC 
AGCAACTTTC ACTACCATGA ACTCAAGCAC ATTGTGTTTG TGGGTTCACT TGAATACCTG 
AGAAGGGAAT GGGAGACACT GCATAACTTC CCCAAGGTTT CAATCTTGCC TGGTACGCCA 
TTAAGTCGGG CTGATTTAAG GGCTGTCAAC ATCAACCTCT GCGACATGTG CGTTATCCTG 
TCAGCCAATC AGAATAATAT TGATGATGCT TCGCTGCAGG ACAAGGAATG CATCTTGGCG 
TCACTCAACA TCAAATCTAT GCAGTTTGAT GACAGCATTG GGGTCTTGCA GGCTAATTCC 
CAAGGCTTTA CACCCCCTGG AATGGATAGG TCATCTCCAG ACAACAGTCC AGTCCATGGC 
CTGTTACGTC AACCCTCCAT TACAACAGGA GCCAACATCC CTATTATTAC AGAGCTAGCT 
AAGCCTGGCA AACTTCTGCC TTTGGTTTCA ATCAGTCAGG AAAAAAACAG TGGAACCCAC 
ATTCTTATGA TAACTGAACT GGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
GCAGAAAAGA AATGGTTTAC AGATGAGCCG GATAATGCCT ATCCCAGAAA CATTCAAATC 
AAGCCCATGA GCACTCACAT GGCCAATCAG ATCAATCAAT ATAAATCAAC AAGCAGCTTG 
ATACCACCAA TCCGAGAAGT TGAAGATGAA TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807023.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662512.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OLe52797
Clone ID Related Accession (Same CDS sequence)	XM_017807023.1
Accession Version	XM_017807023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGTATATTAA TCAATCCTGG AAACCACGTC 
AAGATTCAAG AAGGTACCTT AGGATTCTTC ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG 
GCATATTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA 
TGTGGCTGCA AACGGCTTGA AGATGAGCAG CCATCGACAC TATCACCCAA AAAAAAGCAG 
CGAAACGGAG GCATGAGAAA TTCACCCAAC TCCTCACCCA AGCTGATGAG GCATGACCCC 
TTGTTAATTC CTGGCAATGA GCAGATTGAC AATATGGATG CCAACGTGAA AAAATATGAC 
TCTACAGGGA TGTTTCATTG GTGTCCAGCC AAGGACATTG AAAAGGTCAT TTTGACTCGA 
AGTGAAGCAG CGATGACAGT TTTAAGTGGG CATGTAGTCG TTTGCATATT TGGGGATGTT 
AAATCTGCTT TGATTGGATT AAGAAATTTA GTGATGCCAT TACGAGCCAG CAACTTTCAC 
TACCATGAAC TCAAGCACAT TGTGTTTGTG GGTTCACTTG AATACCTGAG AAGGGAATGG 
GAGACACTGC ATAACTTCCC CAAGGTTTCA ATCTTGCCTG GTACGCCATT AAGTCGGGCT 
GATTTAAGGG CTGTCAACAT CAACCTCTGC GACATGTGCG TTATCCTGTC AGCCAATCAG 
AATAATATTG ATGATGCTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC 
AAATCTATGC AGTTTGATGA CAGCATTGGG GTCTTGCAGG CTAATTCCCA AGGCTTTACA 
CCCCCTGGAA TGGATAGGTC ATCTCCAGAC AACAGTCCAG TCCATGGCCT GTTACGTCAA 
CCCTCCATTA CAACAGGAGC CAACATCCCT ATTATTACAG AGCTAGCTAA GCCTGGCAAA 
CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA AAAAACAGTG GAACCCACAT TCTTATGATA 
ACTGAACTGG TGAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA 
GACACAGAAC TGTATCTCAC ACAGCCCTTT GCATGTGGAA CCGCGTTTGC TGTCAGTGTG 
CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA 
TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCTGAGGA AAATGCATTG 
AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG 
CTGGCTTTGT ATGATGGGCC GTTTGCAGAC CTAGGGGATG GAGGTTGTTA TGGAGATCTA 
TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAT 
GCACATCTAA GCACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCGTAT 
GAATTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTTGA CCACAATGCT 
GGCCAGTCCC GAGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG 
AGCTCATCAG TTCACTCTAT CCCATCAACA GCAAATCGAC CGAACCGCAC CAAGACCAGG 
GATTCCCGGG AAAAACAGAA TGCAACAAGG ATGAATAGAA TGGGCCAAGC AGAAAAGAAA 
TGGTTTACAG ATGAGCCGGA TAATGCCTAT CCCAGAAACA TTCAAATCAA GCCCATGAGC 
ACTCACATGG CCAATCAGAT CAATCAATAT AAATCAACAA GCAGCTTGAT ACCACCAATC 
CGAGAAGTTG AAGATGAATG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662512.1
CDS	355..4098
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807023.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807023.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGTATATTAA TCAATCCTGG AAACCACGTC 
AAGATTCAAG AAGGTACCTT AGGATTCTTC ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG 
GCATATTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA 
TGTGGCTGCA AACGGCTTGA AGATGAGCAG CCATCGACAC TATCACCCAA AAAAAAGCAG 
CGAAACGGAG GCATGAGAAA TTCACCCAAC TCCTCACCCA AGCTGATGAG GCATGACCCC 
TTGTTAATTC CTGGCAATGA GCAGATTGAC AATATGGATG CCAACGTGAA AAAATATGAC 
TCTACAGGGA TGTTTCATTG GTGTCCAGCC AAGGACATTG AAAAGGTCAT TTTGACTCGA 
AGTGAAGCAG CGATGACAGT TTTAAGTGGG CATGTAGTCG TTTGCATATT TGGGGATGTT 
AAATCTGCTT TGATTGGATT AAGAAATTTA GTGATGCCAT TACGAGCCAG CAACTTTCAC 
TACCATGAAC TCAAGCACAT TGTGTTTGTG GGTTCACTTG AATACCTGAG AAGGGAATGG 
GAGACACTGC ATAACTTCCC CAAGGTTTCA ATCTTGCCTG GTACGCCATT AAGTCGGGCT 
GATTTAAGGG CTGTCAACAT CAACCTCTGC GACATGTGCG TTATCCTGTC AGCCAATCAG 
AATAATATTG ATGATGCTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC 
AAATCTATGC AGTTTGATGA CAGCATTGGG GTCTTGCAGG CTAATTCCCA AGGCTTTACA 
CCCCCTGGAA TGGATAGGTC ATCTCCAGAC AACAGTCCAG TCCATGGCCT GTTACGTCAA 
CCCTCCATTA CAACAGGAGC CAACATCCCT ATTATTACAG AGCTAGCTAA GCCTGGCAAA 
CTTCTGCCTT TGGTTTCAAT CAGTCAGGAA AAAAACAGTG GAACCCACAT TCTTATGATA 
ACTGAACTGG TGAATGATTC AAATGTTCAG TTCTTGGACC AAGATGATGA TGATGATCCA 
GACACAGAAC TGTATCTCAC ACAGCCCTTT GCATGTGGAA CCGCGTTTGC TGTCAGTGTG 
CTGGACTCTC TCATGAGCGC AACATACTTC AATGACAATA TCCTCACACT GATACGGACA 
TTGGTAACAG GAGGAGCCAC ACCAGAGCTG GAAGCACTGA TTGCTGAGGA AAATGCATTG 
AGAGGAGGTT ACAGCACGCC CCAGACACTT GCAAACAGGG ACAGGTGTCG AGTTGCTCAG 
CTGGCTTTGT ATGATGGGCC GTTTGCAGAC CTAGGGGATG GAGGTTGTTA TGGAGATCTA 
TTTTGTAAAG CACTGAAAAC ATACAATATG CTTTGCTTTG GAATTTATCG ATTAAGGGAT 
GCACATCTAA GCACTCCCAG CCAATGCACT AAACGTTATG TTATCACAAA CCCACCGTAT 
GAATTTGAGC TTGTGCCGAC AGACTTGATC TTCTGTTTGA TGCAATTTGA CCACAATGCT 
GGCCAGTCCC GAGCCAGTCT TTCTCATTCC TCCCATTCCT CCTATTCTTC CAGCAAGAAG 
AGCTCATCAG TTCACTCTAT CCCATCAACA GCAAATCGAC CGAACCGCAC CAAGACCAGG 
GATTCCCGGG AAAAACAGAA TGCAACAAGG ATGAATAGAA TGGGCCAAGC AGAAAAGAAA 
TGGTTTACAG ATGAGCCGGA TAATGCCTAT CCCAGAAACA TTCAAATCAA GCCCATGAGC 
ACTCACATGG CCAATCAGAT CAATCAATAT AAATCAACAA GCAGCTTGAT ACCACCAATC 
CGAGAAGTTG AAGATGAATG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807024.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662513.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OLe52798
Clone ID Related Accession (Same CDS sequence)	XM_017807024.1
Accession Version	XM_017807024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTGATCT ATTTTGAAGA TGAGCAGCCA TCGACACTAT CACCCAAAAA AAAGCAGCGA 
AACGGAGGCA TGAGAAATTC ACCCAACTCC TCACCCAAGC TGATGAGGCA TGACCCCTTG 
TTAATTCCTG GCAATGAGCA GATTGACAAT ATGGATGCCA ACGTGAAAAA ATATGACTCT 
ACAGGGATGT TTCATTGGTG TCCAGCCAAG GACATTGAAA AGGTCATTTT GACTCGAAGT 
GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA 
TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC 
CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG 
ACACTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC 
CCTGGAATGG ATAGGTCATC TCCAGACAAC AGTCCAGTCC ATGGCCTGTT ACGTCAACCC 
TCCATTACAA CAGGAGCCAA CATCCCTATT ATTACAGAGC TAGCTAAGCC TGGCAAACTT 
CTGCCTTTGG TTTCAATCAG TCAGGAAAAA AACAGTGGAA CCCACATTCT TATGATAACT 
GAACTGGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAATGC AACAAGGATG AATAGAATGG GCCAAGCAGA AAAGAAATGG 
TTTACAGATG AGCCGGATAA TGCCTATCCC AGAAACATTC AAATCAAGCC CATGAGCACT 
CACATGGCCA ATCAGATCAA TCAATATAAA TCAACAAGCA GCTTGATACC ACCAATCCGA 
GAAGTTGAAG ATGAATGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662513.1
CDS	355..4095
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807024.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807024.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTGATCT ATTTTGAAGA TGAGCAGCCA TCGACACTAT CACCCAAAAA AAAGCAGCGA 
AACGGAGGCA TGAGAAATTC ACCCAACTCC TCACCCAAGC TGATGAGGCA TGACCCCTTG 
TTAATTCCTG GCAATGAGCA GATTGACAAT ATGGATGCCA ACGTGAAAAA ATATGACTCT 
ACAGGGATGT TTCATTGGTG TCCAGCCAAG GACATTGAAA AGGTCATTTT GACTCGAAGT 
GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA 
TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC 
CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG 
ACACTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC 
CCTGGAATGG ATAGGTCATC TCCAGACAAC AGTCCAGTCC ATGGCCTGTT ACGTCAACCC 
TCCATTACAA CAGGAGCCAA CATCCCTATT ATTACAGAGC TAGCTAAGCC TGGCAAACTT 
CTGCCTTTGG TTTCAATCAG TCAGGAAAAA AACAGTGGAA CCCACATTCT TATGATAACT 
GAACTGGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAATGC AACAAGGATG AATAGAATGG GCCAAGCAGA AAAGAAATGG 
TTTACAGATG AGCCGGATAA TGCCTATCCC AGAAACATTC AAATCAAGCC CATGAGCACT 
CACATGGCCA ATCAGATCAA TCAATATAAA TCAACAAGCA GCTTGATACC ACCAATCCGA 
GAAGTTGAAG ATGAATGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807025.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662514.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X8, mRNA.

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CloneID	OLe52799
Clone ID Related Accession (Same CDS sequence)	XM_017807025.1
Accession Version	XM_017807025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3729bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTTA TGATAACTGA ACTGGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAG 
CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGCACTCA CATGGCCAAT 
CAGATCAATC AATATAAATC AACAAGCAGC TTGATACCAC CAATCCGAGA AGTTGAAGAT 
GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662514.1
CDS	355..4083
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807025.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807025.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTTA TGATAACTGA ACTGGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAG 
CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGCACTCA CATGGCCAAT 
CAGATCAATC AATATAAATC AACAAGCAGC TTGATACCAC CAATCCGAGA AGTTGAAGAT 
GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807026.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662515.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OLe52800
Clone ID Related Accession (Same CDS sequence)	XM_017807026.1
Accession Version	XM_017807026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTTA TGATAACTGA ACTGGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGAAAAGA AATGGTTTAC AGATGAGCCG 
GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACTCACAT GGCCAATCAG 
ATCAATCAAT ATAAATCAAC AAGCAGCTTG ATACCACCAA TCCGAGAAGT TGAAGATGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662515.1
CDS	355..4080
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807026.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807026.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTTA TGATAACTGA ACTGGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGAAAAGA AATGGTTTAC AGATGAGCCG 
GATAATGCCT ATCCCAGAAA CATTCAAATC AAGCCCATGA GCACTCACAT GGCCAATCAG 
ATCAATCAAT ATAAATCAAC AAGCAGCTTG ATACCACCAA TCCGAGAAGT TGAAGATGAA 
TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807027.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662516.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OLe52801
Clone ID Related Accession (Same CDS sequence)	XM_017807027.1
Accession Version	XM_017807027.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT 
CAGGAAAAAA ACAGTGGAAC CCACATTCTT ATGATAACTG AACTGGTGAA TGATTCAAAT 
GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGACA CAGAACTGTA TCTCACACAG 
CCCTTTGCAT GTGGAACCGC GTTTGCTGTC AGTGTGCTGG ACTCTCTCAT GAGCGCAACA 
TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA 
GAGCTGGAAG CACTGATTGC TGAGGAAAAT GCATTGAGAG GAGGTTACAG CACGCCCCAG 
ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGCTGG CTTTGTATGA TGGGCCGTTT 
GCAGACCTAG GGGATGGAGG TTGTTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC 
AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGATGCAC ATCTAAGCAC TCCCAGCCAA 
TGCACTAAAC GTTATGTTAT CACAAACCCA CCGTATGAAT TTGAGCTTGT GCCGACAGAC 
TTGATCTTCT GTTTGATGCA ATTTGACCAC AATGCTGGCC AGTCCCGAGC CAGTCTTTCT 
CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCTATCCCA 
TCAACAGCAA ATCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA 
ACAAGGATGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT TTACAGATGA GCCGGATAAT 
GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACTC ACATGGCCAA TCAGATCAAT 
CAATATAAAT CAACAAGCAG CTTGATACCA CCAATCCGAG AAGTTGAAGA TGAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662516.1
CDS	355..4074
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807027.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807027.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT 
CAGGAAAAAA ACAGTGGAAC CCACATTCTT ATGATAACTG AACTGGTGAA TGATTCAAAT 
GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGACA CAGAACTGTA TCTCACACAG 
CCCTTTGCAT GTGGAACCGC GTTTGCTGTC AGTGTGCTGG ACTCTCTCAT GAGCGCAACA 
TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA 
GAGCTGGAAG CACTGATTGC TGAGGAAAAT GCATTGAGAG GAGGTTACAG CACGCCCCAG 
ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGCTGG CTTTGTATGA TGGGCCGTTT 
GCAGACCTAG GGGATGGAGG TTGTTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC 
AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGATGCAC ATCTAAGCAC TCCCAGCCAA 
TGCACTAAAC GTTATGTTAT CACAAACCCA CCGTATGAAT TTGAGCTTGT GCCGACAGAC 
TTGATCTTCT GTTTGATGCA ATTTGACCAC AATGCTGGCC AGTCCCGAGC CAGTCTTTCT 
CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCTATCCCA 
TCAACAGCAA ATCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA 
ACAAGGATGA ATAGAATGGG CCAAGCAGAA AAGAAATGGT TTACAGATGA GCCGGATAAT 
GCCTATCCCA GAAACATTCA AATCAAGCCC ATGAGCACTC ACATGGCCAA TCAGATCAAT 
CAATATAAAT CAACAAGCAG CTTGATACCA CCAATCCGAG AAGTTGAAGA TGAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807028.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662517.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OLe52802
Clone ID Related Accession (Same CDS sequence)	XM_017807028.1
Accession Version	XM_017807028.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT 
CAGGAAAAAA ACAGTGGAAC CCACATTCTT ATGATAACTG AACTGGTGAA TGATTCAAAT 
GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGACA CAGAACTGTA TCTCACACAG 
CCCTTTGCAT GTGGAACCGC GTTTGCTGTC AGTGTGCTGG ACTCTCTCAT GAGCGCAACA 
TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA 
GAGCTGGAAG CACTGATTGC TGAGGAAAAT GCATTGAGAG GAGGTTACAG CACGCCCCAG 
ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGCTGG CTTTGTATGA TGGGCCGTTT 
GCAGACCTAG GGGATGGAGG TTGTTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC 
AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGATGCAC ATCTAAGCAC TCCCAGCCAA 
TGCACTAAAC GTTATGTTAT CACAAACCCA CCGTATGAAT TTGAGCTTGT GCCGACAGAC 
TTGATCTTCT GTTTGATGCA ATTTGACCAC AATGCTGGCC AGTCCCGAGC CAGTCTTTCT 
CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCTATCCCA 
TCAACAGCAA ATCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA 
ACAAGGATGA ATAGAATGGG CCAAGAAAAG AAATGGTTTA CAGATGAGCC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACTCACA TGGCCAATCA GATCAATCAA 
TATAAATCAA CAAGCAGCTT GATACCACCA ATCCGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662517.1
CDS	355..4071
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807028.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807028.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGCTAAGCCT GGCAAACTTC TGCCTTTGGT TTCAATCAGT 
CAGGAAAAAA ACAGTGGAAC CCACATTCTT ATGATAACTG AACTGGTGAA TGATTCAAAT 
GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGACA CAGAACTGTA TCTCACACAG 
CCCTTTGCAT GTGGAACCGC GTTTGCTGTC AGTGTGCTGG ACTCTCTCAT GAGCGCAACA 
TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA 
GAGCTGGAAG CACTGATTGC TGAGGAAAAT GCATTGAGAG GAGGTTACAG CACGCCCCAG 
ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGCTGG CTTTGTATGA TGGGCCGTTT 
GCAGACCTAG GGGATGGAGG TTGTTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC 
AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGATGCAC ATCTAAGCAC TCCCAGCCAA 
TGCACTAAAC GTTATGTTAT CACAAACCCA CCGTATGAAT TTGAGCTTGT GCCGACAGAC 
TTGATCTTCT GTTTGATGCA ATTTGACCAC AATGCTGGCC AGTCCCGAGC CAGTCTTTCT 
CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCTATCCCA 
TCAACAGCAA ATCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA 
ACAAGGATGA ATAGAATGGG CCAAGAAAAG AAATGGTTTA CAGATGAGCC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACTCACA TGGCCAATCA GATCAATCAA 
TATAAATCAA CAAGCAGCTT GATACCACCA ATCCGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807029.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662518.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OLe52803
Clone ID Related Accession (Same CDS sequence)	XM_017807029.1
Accession Version	XM_017807029.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAATGC AACAAGGATG AATAGAATGG GCCAAGAAAA GAAATGGTTT 
ACAGATGAGC CGGATAATGC CTATCCCAGA AACATTCAAA TCAAGCCCAT GAGCACTCAC 
ATGGCCAATC AGATCAATCA ATATAAATCA ACAAGCAGCT TGATACCACC AATCCGAGAA 
GTTGAAGATG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662518.1
CDS	355..4032
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807029.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807029.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAATGC AACAAGGATG AATAGAATGG GCCAAGAAAA GAAATGGTTT 
ACAGATGAGC CGGATAATGC CTATCCCAGA AACATTCAAA TCAAGCCCAT GAGCACTCAC 
ATGGCCAATC AGATCAATCA ATATAAATCA ACAAGCAGCT TGATACCACC AATCCGAGAA 
GTTGAAGATG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807030.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662519.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X13, mRNA.

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CloneID	OLe52804
Clone ID Related Accession (Same CDS sequence)	XM_017807030.1
Accession Version	XM_017807030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGTATATTAA TCAATCCTGG AAACCACGTC 
AAGATTCAAG AAGGTACCTT AGGATTCTTC ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG 
GCATATTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA 
TGTGGCTGCA AACGGCTGAT CTATTTTGAA GATGAGCAGC CATCGACACT ATCACCCAAA 
AAAAAGCAGC GAAACGGAGG CATGAGAAAT TCACCCAACT CCTCACCCAA GCTGATGAGG 
CATGACCCCT TGTTAATTCC TGGCAATGAG CAGATTGACA ATATGGATGC CAACGTGAAA 
AAATATGACT CTACAGGGAT GTTTCATTGG TGTCCAGCCA AGGACATTGA AAAGGTCATT 
TTGACTCGAA GTGAAGCAGC GATGACAGTT TTAAGTGGGC ATGTAGTCGT TTGCATATTT 
GGGGATGTTA AATCTGCTTT GATTGGATTA AGAAATTTAG TGATGCCATT ACGAGCCAGC 
AACTTTCACT ACCATGAACT CAAGCACATT GTGTTTGTGG GTTCACTTGA ATACCTGAGA 
AGGGAATGGG AGACACTGCA TAACTTCCCC AAGGTTTCAA TCTTGCCTGG TACGCCATTA 
AGTCGGGCTG ATTTAAGGGC TGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA 
GCCAATCAGA ATAATATTGA TGATGCTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA 
CTCAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA 
GGCTTTACAC CCCCTGGAAT GGATAGGTCA TCTCCAGACA ACAGTCCAGT CCATGGCCTG 
TTACGTCAAC CCTCCATTAC AACAGGAGCC AACATCCCTA TTATTACAGA GCTAGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAG 
CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGCACTCA CATGGCCAAT 
CAGATCAATC AATATAAATC AACAAGCAGC TTGATACCAC CAATCCGAGA AGTTGAAGAT 
GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662519.1
CDS	355..4023
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807030.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807030.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGTATATTAA TCAATCCTGG AAACCACGTC 
AAGATTCAAG AAGGTACCTT AGGATTCTTC ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG 
GCATATTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA 
TGTGGCTGCA AACGGCTGAT CTATTTTGAA GATGAGCAGC CATCGACACT ATCACCCAAA 
AAAAAGCAGC GAAACGGAGG CATGAGAAAT TCACCCAACT CCTCACCCAA GCTGATGAGG 
CATGACCCCT TGTTAATTCC TGGCAATGAG CAGATTGACA ATATGGATGC CAACGTGAAA 
AAATATGACT CTACAGGGAT GTTTCATTGG TGTCCAGCCA AGGACATTGA AAAGGTCATT 
TTGACTCGAA GTGAAGCAGC GATGACAGTT TTAAGTGGGC ATGTAGTCGT TTGCATATTT 
GGGGATGTTA AATCTGCTTT GATTGGATTA AGAAATTTAG TGATGCCATT ACGAGCCAGC 
AACTTTCACT ACCATGAACT CAAGCACATT GTGTTTGTGG GTTCACTTGA ATACCTGAGA 
AGGGAATGGG AGACACTGCA TAACTTCCCC AAGGTTTCAA TCTTGCCTGG TACGCCATTA 
AGTCGGGCTG ATTTAAGGGC TGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA 
GCCAATCAGA ATAATATTGA TGATGCTTCG CTGCAGGACA AGGAATGCAT CTTGGCGTCA 
CTCAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA 
GGCTTTACAC CCCCTGGAAT GGATAGGTCA TCTCCAGACA ACAGTCCAGT CCATGGCCTG 
TTACGTCAAC CCTCCATTAC AACAGGAGCC AACATCCCTA TTATTACAGA GCTAGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAATGCAA CAAGGATGAA TAGAATGGGC CAAGCAGAAA AGAAATGGTT TACAGATGAG 
CCGGATAATG CCTATCCCAG AAACATTCAA ATCAAGCCCA TGAGCACTCA CATGGCCAAT 
CAGATCAATC AATATAAATC AACAAGCAGC TTGATACCAC CAATCCGAGA AGTTGAAGAT 
GAATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807032.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662521.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OLe52805
Clone ID Related Accession (Same CDS sequence)	XM_017807032.1
Accession Version	XM_017807032.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTGATCT ATTTTGAAGA TGAGCAGCCA TCGACACTAT CACCCAAAAA AAAGCAGCGA 
AACGGAGGCA TGAGAAATTC ACCCAACTCC TCACCCAAGC TGATGAGGCA TGACCCCTTG 
TTAATTCCTG GCAATGAGCA GATTGACAAT ATGGATGCCA ACGTGAAAAA ATATGACTCT 
ACAGGGATGT TTCATTGGTG TCCAGCCAAG GACATTGAAA AGGTCATTTT GACTCGAAGT 
GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA 
TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC 
CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG 
ACACTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC 
CCTGGAATGG ATAGGTCATC TCCAGACAAC AGTCCAGTCC ATGGCCTGTT ACGTCAACCC 
TCCATTACAA CAGGAGCCAA CATCCCTATT ATTACAGAGC TAGTGAATGA TTCAAATGTT 
CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGACACAG AACTGTATCT CACACAGCCC 
TTTGCATGTG GAACCGCGTT TGCTGTCAGT GTGCTGGACT CTCTCATGAG CGCAACATAC 
TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG 
CTGGAAGCAC TGATTGCTGA GGAAAATGCA TTGAGAGGAG GTTACAGCAC GCCCCAGACA 
CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGCTGGCTT TGTATGATGG GCCGTTTGCA 
GACCTAGGGG ATGGAGGTTG TTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT 
ATGCTTTGCT TTGGAATTTA TCGATTAAGG GATGCACATC TAAGCACTCC CAGCCAATGC 
ACTAAACGTT ATGTTATCAC AAACCCACCG TATGAATTTG AGCTTGTGCC GACAGACTTG 
ATCTTCTGTT TGATGCAATT TGACCACAAT GCTGGCCAGT CCCGAGCCAG TCTTTCTCAT 
TCCTCCCATT CCTCCTATTC TTCCAGCAAG AAGAGCTCAT CAGTTCACTC TATCCCATCA 
ACAGCAAATC GACCGAACCG CACCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA 
AGGATGAATA GAATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAGCC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACTCACA TGGCCAATCA GATCAATCAA 
TATAAATCAA CAAGCAGCTT GATACCACCA ATCCGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662521.1
CDS	355..4011
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807032.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807032.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTGATCT ATTTTGAAGA TGAGCAGCCA TCGACACTAT CACCCAAAAA AAAGCAGCGA 
AACGGAGGCA TGAGAAATTC ACCCAACTCC TCACCCAAGC TGATGAGGCA TGACCCCTTG 
TTAATTCCTG GCAATGAGCA GATTGACAAT ATGGATGCCA ACGTGAAAAA ATATGACTCT 
ACAGGGATGT TTCATTGGTG TCCAGCCAAG GACATTGAAA AGGTCATTTT GACTCGAAGT 
GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA 
TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC 
CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG 
ACACTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC 
CCTGGAATGG ATAGGTCATC TCCAGACAAC AGTCCAGTCC ATGGCCTGTT ACGTCAACCC 
TCCATTACAA CAGGAGCCAA CATCCCTATT ATTACAGAGC TAGTGAATGA TTCAAATGTT 
CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGACACAG AACTGTATCT CACACAGCCC 
TTTGCATGTG GAACCGCGTT TGCTGTCAGT GTGCTGGACT CTCTCATGAG CGCAACATAC 
TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG 
CTGGAAGCAC TGATTGCTGA GGAAAATGCA TTGAGAGGAG GTTACAGCAC GCCCCAGACA 
CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGCTGGCTT TGTATGATGG GCCGTTTGCA 
GACCTAGGGG ATGGAGGTTG TTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT 
ATGCTTTGCT TTGGAATTTA TCGATTAAGG GATGCACATC TAAGCACTCC CAGCCAATGC 
ACTAAACGTT ATGTTATCAC AAACCCACCG TATGAATTTG AGCTTGTGCC GACAGACTTG 
ATCTTCTGTT TGATGCAATT TGACCACAAT GCTGGCCAGT CCCGAGCCAG TCTTTCTCAT 
TCCTCCCATT CCTCCTATTC TTCCAGCAAG AAGAGCTCAT CAGTTCACTC TATCCCATCA 
ACAGCAAATC GACCGAACCG CACCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA 
AGGATGAATA GAATGGGCCA AGCAGAAAAG AAATGGTTTA CAGATGAGCC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACTCACA TGGCCAATCA GATCAATCAA 
TATAAATCAA CAAGCAGCTT GATACCACCA ATCCGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807033.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662522.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OLe52806
Clone ID Related Accession (Same CDS sequence)	XM_017807033.1
Accession Version	XM_017807033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGTATATTAA TCAATCCTGG AAACCACGTC 
AAGATTCAAG AAGGTACCTT AGGATTCTTC ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG 
GCATATTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA 
TGTGGCTGCA AACGGCTTGA AGATGAGCAG CCATCGACAC TATCACCCAA AAAAAAGCAG 
CGAAACGGAG GCATGAGAAA TTCACCCAAC TCCTCACCCA AGCTGATGAG GCATGACCCC 
TTGTTAATTC CTGGCAATGA GCAGATTGAC AATATGGATG CCAACGTGAA AAAATATGAC 
TCTACAGGGA TGTTTCATTG GTGTCCAGCC AAGGACATTG AAAAGGTCAT TTTGACTCGA 
AGTGAAGCAG CGATGACAGT TTTAAGTGGG CATGTAGTCG TTTGCATATT TGGGGATGTT 
AAATCTGCTT TGATTGGATT AAGAAATTTA GTGATGCCAT TACGAGCCAG CAACTTTCAC 
TACCATGAAC TCAAGCACAT TGTGTTTGTG GGTTCACTTG AATACCTGAG AAGGGAATGG 
GAGACACTGC ATAACTTCCC CAAGGTTTCA ATCTTGCCTG GTACGCCATT AAGTCGGGCT 
GATTTAAGGG CTGTCAACAT CAACCTCTGC GACATGTGCG TTATCCTGTC AGCCAATCAG 
AATAATATTG ATGATGCTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC 
AAATCTATGC AGTTTGATGA CAGCATTGGG GTCTTGCAGG CTAATTCCCA AGGCTTTACA 
CCCCCTGGAA TGGATAGGTC ATCTCCAGAC AACAGTCCAG TCCATGGCCT GTTACGTCAA 
CCCTCCATTA CAACAGGAGC CAACATCCCT ATTATTACAG AGCTAGTGAA TGATTCAAAT 
GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGACA CAGAACTGTA TCTCACACAG 
CCCTTTGCAT GTGGAACCGC GTTTGCTGTC AGTGTGCTGG ACTCTCTCAT GAGCGCAACA 
TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA 
GAGCTGGAAG CACTGATTGC TGAGGAAAAT GCATTGAGAG GAGGTTACAG CACGCCCCAG 
ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGCTGG CTTTGTATGA TGGGCCGTTT 
GCAGACCTAG GGGATGGAGG TTGTTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC 
AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGATGCAC ATCTAAGCAC TCCCAGCCAA 
TGCACTAAAC GTTATGTTAT CACAAACCCA CCGTATGAAT TTGAGCTTGT GCCGACAGAC 
TTGATCTTCT GTTTGATGCA ATTTGACCAC AATGCTGGCC AGTCCCGAGC CAGTCTTTCT 
CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCTATCCCA 
TCAACAGCAA ATCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA 
ACAAGGATGA ATAGAATGGG CCAAGAAAAG AAATGGTTTA CAGATGAGCC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACTCACA TGGCCAATCA GATCAATCAA 
TATAAATCAA CAAGCAGCTT GATACCACCA ATCCGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662522.1
CDS	355..4011
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807033.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807033.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGTATATTAA TCAATCCTGG AAACCACGTC 
AAGATTCAAG AAGGTACCTT AGGATTCTTC ATTGCAAGTG ATGCCAAAGA AGTAAAAAGG 
GCATATTTTT ACTGCAAGGC CTGTCATGAT GACATCACGG ATCCCAAAAG GATAAAAAAA 
TGTGGCTGCA AACGGCTTGA AGATGAGCAG CCATCGACAC TATCACCCAA AAAAAAGCAG 
CGAAACGGAG GCATGAGAAA TTCACCCAAC TCCTCACCCA AGCTGATGAG GCATGACCCC 
TTGTTAATTC CTGGCAATGA GCAGATTGAC AATATGGATG CCAACGTGAA AAAATATGAC 
TCTACAGGGA TGTTTCATTG GTGTCCAGCC AAGGACATTG AAAAGGTCAT TTTGACTCGA 
AGTGAAGCAG CGATGACAGT TTTAAGTGGG CATGTAGTCG TTTGCATATT TGGGGATGTT 
AAATCTGCTT TGATTGGATT AAGAAATTTA GTGATGCCAT TACGAGCCAG CAACTTTCAC 
TACCATGAAC TCAAGCACAT TGTGTTTGTG GGTTCACTTG AATACCTGAG AAGGGAATGG 
GAGACACTGC ATAACTTCCC CAAGGTTTCA ATCTTGCCTG GTACGCCATT AAGTCGGGCT 
GATTTAAGGG CTGTCAACAT CAACCTCTGC GACATGTGCG TTATCCTGTC AGCCAATCAG 
AATAATATTG ATGATGCTTC GCTGCAGGAC AAGGAATGCA TCTTGGCGTC ACTCAACATC 
AAATCTATGC AGTTTGATGA CAGCATTGGG GTCTTGCAGG CTAATTCCCA AGGCTTTACA 
CCCCCTGGAA TGGATAGGTC ATCTCCAGAC AACAGTCCAG TCCATGGCCT GTTACGTCAA 
CCCTCCATTA CAACAGGAGC CAACATCCCT ATTATTACAG AGCTAGTGAA TGATTCAAAT 
GTTCAGTTCT TGGACCAAGA TGATGATGAT GATCCAGACA CAGAACTGTA TCTCACACAG 
CCCTTTGCAT GTGGAACCGC GTTTGCTGTC AGTGTGCTGG ACTCTCTCAT GAGCGCAACA 
TACTTCAATG ACAATATCCT CACACTGATA CGGACATTGG TAACAGGAGG AGCCACACCA 
GAGCTGGAAG CACTGATTGC TGAGGAAAAT GCATTGAGAG GAGGTTACAG CACGCCCCAG 
ACACTTGCAA ACAGGGACAG GTGTCGAGTT GCTCAGCTGG CTTTGTATGA TGGGCCGTTT 
GCAGACCTAG GGGATGGAGG TTGTTATGGA GATCTATTTT GTAAAGCACT GAAAACATAC 
AATATGCTTT GCTTTGGAAT TTATCGATTA AGGGATGCAC ATCTAAGCAC TCCCAGCCAA 
TGCACTAAAC GTTATGTTAT CACAAACCCA CCGTATGAAT TTGAGCTTGT GCCGACAGAC 
TTGATCTTCT GTTTGATGCA ATTTGACCAC AATGCTGGCC AGTCCCGAGC CAGTCTTTCT 
CATTCCTCCC ATTCCTCCTA TTCTTCCAGC AAGAAGAGCT CATCAGTTCA CTCTATCCCA 
TCAACAGCAA ATCGACCGAA CCGCACCAAG ACCAGGGATT CCCGGGAAAA ACAGAATGCA 
ACAAGGATGA ATAGAATGGG CCAAGAAAAG AAATGGTTTA CAGATGAGCC GGATAATGCC 
TATCCCAGAA ACATTCAAAT CAAGCCCATG AGCACTCACA TGGCCAATCA GATCAATCAA 
TATAAATCAA CAAGCAGCTT GATACCACCA ATCCGAGAAG TTGAAGATGA ATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807034.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662523.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X16, mRNA.

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CloneID	OLe52807
Clone ID Related Accession (Same CDS sequence)	XM_017807034.1
Accession Version	XM_017807034.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTGATCT ATTTTGAAGA TGAGCAGCCA TCGACACTAT CACCCAAAAA AAAGCAGCGA 
AACGGAGGCA TGAGAAATTC ACCCAACTCC TCACCCAAGC TGATGAGGCA TGACCCCTTG 
TTAATTCCTG GCAATGAGCA GATTGACAAT ATGGATGCCA ACGTGAAAAA ATATGACTCT 
ACAGGGATGT TTCATTGGTG TCCAGCCAAG GACATTGAAA AGGTCATTTT GACTCGAAGT 
GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA 
TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC 
CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG 
ACACTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC 
CCTGGAATGG ATAGGTCATC TCCAGACAAC AGTCCAGTCC ATGGCCTGTT ACGTCAACCC 
TCCATTACAA CAGGAGCCAA CATCCCTATT ATTACAGAGC TAGTGAATGA TTCAAATGTT 
CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGACACAG AACTGTATCT CACACAGCCC 
TTTGCATGTG GAACCGCGTT TGCTGTCAGT GTGCTGGACT CTCTCATGAG CGCAACATAC 
TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG 
CTGGAAGCAC TGATTGCTGA GGAAAATGCA TTGAGAGGAG GTTACAGCAC GCCCCAGACA 
CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGCTGGCTT TGTATGATGG GCCGTTTGCA 
GACCTAGGGG ATGGAGGTTG TTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT 
ATGCTTTGCT TTGGAATTTA TCGATTAAGG GATGCACATC TAAGCACTCC CAGCCAATGC 
ACTAAACGTT ATGTTATCAC AAACCCACCG TATGAATTTG AGCTTGTGCC GACAGACTTG 
ATCTTCTGTT TGATGCAATT TGACCACAAT GCTGGCCAGT CCCGAGCCAG TCTTTCTCAT 
TCCTCCCATT CCTCCTATTC TTCCAGCAAG AAGAGCTCAT CAGTTCACTC TATCCCATCA 
ACAGCAAATC GACCGAACCG CACCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA 
AGGATGAATA GAATGGGCCA AGAAAAGAAA TGGTTTACAG ATGAGCCGGA TAATGCCTAT 
CCCAGAAACA TTCAAATCAA GCCCATGAGC ACTCACATGG CCAATCAGAT CAATCAATAT 
AAATCAACAA GCAGCTTGAT ACCACCAATC CGAGAAGTTG AAGATGAATG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662523.1
CDS	355..4008
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807034.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807034.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTGATCT ATTTTGAAGA TGAGCAGCCA TCGACACTAT CACCCAAAAA AAAGCAGCGA 
AACGGAGGCA TGAGAAATTC ACCCAACTCC TCACCCAAGC TGATGAGGCA TGACCCCTTG 
TTAATTCCTG GCAATGAGCA GATTGACAAT ATGGATGCCA ACGTGAAAAA ATATGACTCT 
ACAGGGATGT TTCATTGGTG TCCAGCCAAG GACATTGAAA AGGTCATTTT GACTCGAAGT 
GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA 
TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC 
CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG 
ACACTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC 
CCTGGAATGG ATAGGTCATC TCCAGACAAC AGTCCAGTCC ATGGCCTGTT ACGTCAACCC 
TCCATTACAA CAGGAGCCAA CATCCCTATT ATTACAGAGC TAGTGAATGA TTCAAATGTT 
CAGTTCTTGG ACCAAGATGA TGATGATGAT CCAGACACAG AACTGTATCT CACACAGCCC 
TTTGCATGTG GAACCGCGTT TGCTGTCAGT GTGCTGGACT CTCTCATGAG CGCAACATAC 
TTCAATGACA ATATCCTCAC ACTGATACGG ACATTGGTAA CAGGAGGAGC CACACCAGAG 
CTGGAAGCAC TGATTGCTGA GGAAAATGCA TTGAGAGGAG GTTACAGCAC GCCCCAGACA 
CTTGCAAACA GGGACAGGTG TCGAGTTGCT CAGCTGGCTT TGTATGATGG GCCGTTTGCA 
GACCTAGGGG ATGGAGGTTG TTATGGAGAT CTATTTTGTA AAGCACTGAA AACATACAAT 
ATGCTTTGCT TTGGAATTTA TCGATTAAGG GATGCACATC TAAGCACTCC CAGCCAATGC 
ACTAAACGTT ATGTTATCAC AAACCCACCG TATGAATTTG AGCTTGTGCC GACAGACTTG 
ATCTTCTGTT TGATGCAATT TGACCACAAT GCTGGCCAGT CCCGAGCCAG TCTTTCTCAT 
TCCTCCCATT CCTCCTATTC TTCCAGCAAG AAGAGCTCAT CAGTTCACTC TATCCCATCA 
ACAGCAAATC GACCGAACCG CACCAAGACC AGGGATTCCC GGGAAAAACA GAATGCAACA 
AGGATGAATA GAATGGGCCA AGAAAAGAAA TGGTTTACAG ATGAGCCGGA TAATGCCTAT 
CCCAGAAACA TTCAAATCAA GCCCATGAGC ACTCACATGG CCAATCAGAT CAATCAATAT 
AAATCAACAA GCAGCTTGAT ACCACCAATC CGAGAAGTTG AAGATGAATG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807035.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662524.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OLe52808
Clone ID Related Accession (Same CDS sequence)	XM_017807035.1
Accession Version	XM_017807035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGTGAATG ATTCAAATGT TCAGTTCTTG 
GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TCACACAGCC CTTTGCATGT 
GGAACCGCGT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
CTGATTGCTG AGGAAAATGC ATTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
AGGGACAGGT GTCGAGTTGC TCAGCTGGCT TTGTATGATG GGCCGTTTGC AGACCTAGGG 
GATGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
TTTGGAATTT ATCGATTAAG GGATGCACAT CTAAGCACTC CCAGCCAATG CACTAAACGT 
TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
TTGATGCAAT TTGACCACAA TGCTGGCCAG TCCCGAGCCA GTCTTTCTCA TTCCTCCCAT 
TCCTCCTATT CTTCCAGCAA GAAGAGCTCA TCAGTTCACT CTATCCCATC AACAGCAAAT 
CGACCGAACC GCACCAAGAC CAGGGATTCC CGGGAAAAAC AGAATGCAAC AAGGATGAAT 
AGAATGGGCC AAGCAGAAAA GAAATGGTTT ACAGATGAGC CGGATAATGC CTATCCCAGA 
AACATTCAAA TCAAGCCCAT GAGCACTCAC ATGGCCAATC AGATCAATCA ATATAAATCA 
ACAAGCAGCT TGATACCACC AATCCGAGAA GTTGAAGATG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662524.1
CDS	355..4002
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807035.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807035.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGTGAATG ATTCAAATGT TCAGTTCTTG 
GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TCACACAGCC CTTTGCATGT 
GGAACCGCGT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
CTGATTGCTG AGGAAAATGC ATTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
AGGGACAGGT GTCGAGTTGC TCAGCTGGCT TTGTATGATG GGCCGTTTGC AGACCTAGGG 
GATGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
TTTGGAATTT ATCGATTAAG GGATGCACAT CTAAGCACTC CCAGCCAATG CACTAAACGT 
TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
TTGATGCAAT TTGACCACAA TGCTGGCCAG TCCCGAGCCA GTCTTTCTCA TTCCTCCCAT 
TCCTCCTATT CTTCCAGCAA GAAGAGCTCA TCAGTTCACT CTATCCCATC AACAGCAAAT 
CGACCGAACC GCACCAAGAC CAGGGATTCC CGGGAAAAAC AGAATGCAAC AAGGATGAAT 
AGAATGGGCC AAGCAGAAAA GAAATGGTTT ACAGATGAGC CGGATAATGC CTATCCCAGA 
AACATTCAAA TCAAGCCCAT GAGCACTCAC ATGGCCAATC AGATCAATCA ATATAAATCA 
ACAAGCAGCT TGATACCACC AATCCGAGAA GTTGAAGATG AATGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807036.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662525.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OLe52809
Clone ID Related Accession (Same CDS sequence)	XM_017807036.1
Accession Version	XM_017807036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAGCCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG CACTCACATG GCCAATCAGA TCAATCAATA TAAATCAACA 
AGCAGCTTGA TACCACCAAT CCGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662525.1
CDS	355..3999
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807036.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807036.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
ATGGGCCAAG CAGAAAAGAA ATGGTTTACA GATGAGCCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG CACTCACATG GCCAATCAGA TCAATCAATA TAAATCAACA 
AGCAGCTTGA TACCACCAAT CCGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807037.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662526.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X19, mRNA.

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CloneID	OLe52810
Clone ID Related Accession (Same CDS sequence)	XM_017807037.1
Accession Version	XM_017807037.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3645bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGTGAATG ATTCAAATGT TCAGTTCTTG 
GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TCACACAGCC CTTTGCATGT 
GGAACCGCGT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
CTGATTGCTG AGGAAAATGC ATTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
AGGGACAGGT GTCGAGTTGC TCAGCTGGCT TTGTATGATG GGCCGTTTGC AGACCTAGGG 
GATGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
TTTGGAATTT ATCGATTAAG GGATGCACAT CTAAGCACTC CCAGCCAATG CACTAAACGT 
TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
TTGATGCAAT TTGACCACAA TGCTGGCCAG TCCCGAGCCA GTCTTTCTCA TTCCTCCCAT 
TCCTCCTATT CTTCCAGCAA GAAGAGCTCA TCAGTTCACT CTATCCCATC AACAGCAAAT 
CGACCGAACC GCACCAAGAC CAGGGATTCC CGGGAAAAAC AGAATGCAAC AAGGATGAAT 
AGAATGGGCC AAGAAAAGAA ATGGTTTACA GATGAGCCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG CACTCACATG GCCAATCAGA TCAATCAATA TAAATCAACA 
AGCAGCTTGA TACCACCAAT CCGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662526.1
CDS	355..3999
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807037.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807037.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGTGAATG ATTCAAATGT TCAGTTCTTG 
GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TCACACAGCC CTTTGCATGT 
GGAACCGCGT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
CTGATTGCTG AGGAAAATGC ATTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
AGGGACAGGT GTCGAGTTGC TCAGCTGGCT TTGTATGATG GGCCGTTTGC AGACCTAGGG 
GATGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
TTTGGAATTT ATCGATTAAG GGATGCACAT CTAAGCACTC CCAGCCAATG CACTAAACGT 
TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
TTGATGCAAT TTGACCACAA TGCTGGCCAG TCCCGAGCCA GTCTTTCTCA TTCCTCCCAT 
TCCTCCTATT CTTCCAGCAA GAAGAGCTCA TCAGTTCACT CTATCCCATC AACAGCAAAT 
CGACCGAACC GCACCAAGAC CAGGGATTCC CGGGAAAAAC AGAATGCAAC AAGGATGAAT 
AGAATGGGCC AAGAAAAGAA ATGGTTTACA GATGAGCCGG ATAATGCCTA TCCCAGAAAC 
ATTCAAATCA AGCCCATGAG CACTCACATG GCCAATCAGA TCAATCAATA TAAATCAACA 
AGCAGCTTGA TACCACCAAT CCGAGAAGTT GAAGATGAAT GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807038.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662527.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OLe52811
Clone ID Related Accession (Same CDS sequence)	XM_017807038.1
Accession Version	XM_017807038.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAGCCGGATA ATGCCTATCC CAGAAACATT 
CAAATCAAGC CCATGAGCAC TCACATGGCC AATCAGATCA ATCAATATAA ATCAACAAGC 
AGCTTGATAC CACCAATCCG AGAAGTTGAA GATGAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662527.1
CDS	355..3996
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807038.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807038.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ATGCAACAAG GATGAATAGA 
ATGGGCCAAG AAAAGAAATG GTTTACAGAT GAGCCGGATA ATGCCTATCC CAGAAACATT 
CAAATCAAGC CCATGAGCAC TCACATGGCC AATCAGATCA ATCAATATAA ATCAACAAGC 
AGCTTGATAC CACCAATCCG AGAAGTTGAA GATGAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807039.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662528.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OLe52812
Clone ID Related Accession (Same CDS sequence)	XM_017807039.1
Accession Version	XM_017807039.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
GCAGAAAAGA AATGGTTTAC AGATGAGCCG GATAATGCCT ATCCCAGAAA CATTCAAATC 
AAGCCCATGA GCACTCACAT GGCCAATCAG ATCAATCAAT ATAAATCAAC AAGCAGCTTG 
ATACCACCAA TCCGAGAAGT TGAAGATGAA TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662528.1
CDS	355..3990
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQAEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807039.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807039.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
GCAGAAAAGA AATGGTTTAC AGATGAGCCG GATAATGCCT ATCCCAGAAA CATTCAAATC 
AAGCCCATGA GCACTCACAT GGCCAATCAG ATCAATCAAT ATAAATCAAC AAGCAGCTTG 
ATACCACCAA TCCGAGAAGT TGAAGATGAA TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807040.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662529.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X22, mRNA.

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CloneID	OLe52813
Clone ID Related Accession (Same CDS sequence)	XM_017807040.1
Accession Version	XM_017807040.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
GAAAAGAAAT GGTTTACAGA TGAGCCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG 
CCCATGAGCA CTCACATGGC CAATCAGATC AATCAATATA AATCAACAAG CAGCTTGATA 
CCACCAATCC GAGAAGTTGA AGATGAATGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662529.1
CDS	355..3987
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNATRMNRMGQEKKWFTDEPDNAYPRNIQIKPMSTHMANQINQYKSTSSLIPPIREVEDEC

Target ORF information:

RefSeq Version	XM_017807040.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807040.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AATGCAACAA GGATGAATAG AATGGGCCAA 
GAAAAGAAAT GGTTTACAGA TGAGCCGGAT AATGCCTATC CCAGAAACAT TCAAATCAAG 
CCCATGAGCA CTCACATGGC CAATCAGATC AATCAATATA AATCAACAAG CAGCTTGATA 
CCACCAATCC GAGAAGTTGA AGATGAATGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807041.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662530.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OLe52814
Clone ID Related Accession (Same CDS sequence)	XM_017807041.1
Accession Version	XM_017807041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT TTGGTTTCAA TCAGTCAGGA AAAAAACAGT 
GGAACCCACA TTCTTATGAT AACTGAACTG GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ACAGAAAAGA AATGGTTTAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662530.1
CDS	355..3960
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_017807041.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807041.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT TTGGTTTCAA TCAGTCAGGA AAAAAACAGT 
GGAACCCACA TTCTTATGAT AACTGAACTG GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA ACAGAAAAGA AATGGTTTAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807042.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662531.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OLe52815
Clone ID Related Accession (Same CDS sequence)	XM_017807042.1
Accession Version	XM_017807042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT TTGGTTTCAA TCAGTCAGGA AAAAAACAGT 
GGAACCCACA TTCTTATGAT AACTGAACTG GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA AAAAAGAAAT GGTTTACAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662531.1
CDS	355..3957
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017807042.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807042.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGCTA AGCCTGGCAA ACTTCTGCCT TTGGTTTCAA TCAGTCAGGA AAAAAACAGT 
GGAACCCACA TTCTTATGAT AACTGAACTG GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA AAAAAGAAAT GGTTTACAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807043.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662532.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OLe52816
Clone ID Related Accession (Same CDS sequence)	XM_017807043.1
Accession Version	XM_017807043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTGATCT ATTTTGAAGA TGAGCAGCCA TCGACACTAT CACCCAAAAA AAAGCAGCGA 
AACGGAGGCA TGAGAAATTC ACCCAACTCC TCACCCAAGC TGATGAGGCA TGACCCCTTG 
TTAATTCCTG GCAATGAGCA GATTGACAAT ATGGATGCCA ACGTGAAAAA ATATGACTCT 
ACAGGGATGT TTCATTGGTG TCCAGCCAAG GACATTGAAA AGGTCATTTT GACTCGAAGT 
GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA 
TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC 
CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG 
ACACTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC 
CCTGGAATGG ATAGGTCATC TCCAGACAAC AGTCCAGTCC ATGGCCTGTT ACGTCAACCC 
TCCATTACAA CAGGAGCCAA CATCCCTATT ATTACAGAGC TAGCTAAGCC TGGCAAACTT 
CTGCCTTTGG TTTCAATCAG TCAGGAAAAA AACAGTGGAA CCCACATTCT TATGATAACT 
GAACTGGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAACAG AAAAGAAATG GTTTACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662532.1
CDS	355..3936
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_017807043.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807043.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTGATCT ATTTTGAAGA TGAGCAGCCA TCGACACTAT CACCCAAAAA AAAGCAGCGA 
AACGGAGGCA TGAGAAATTC ACCCAACTCC TCACCCAAGC TGATGAGGCA TGACCCCTTG 
TTAATTCCTG GCAATGAGCA GATTGACAAT ATGGATGCCA ACGTGAAAAA ATATGACTCT 
ACAGGGATGT TTCATTGGTG TCCAGCCAAG GACATTGAAA AGGTCATTTT GACTCGAAGT 
GAAGCAGCGA TGACAGTTTT AAGTGGGCAT GTAGTCGTTT GCATATTTGG GGATGTTAAA 
TCTGCTTTGA TTGGATTAAG AAATTTAGTG ATGCCATTAC GAGCCAGCAA CTTTCACTAC 
CATGAACTCA AGCACATTGT GTTTGTGGGT TCACTTGAAT ACCTGAGAAG GGAATGGGAG 
ACACTGCATA ACTTCCCCAA GGTTTCAATC TTGCCTGGTA CGCCATTAAG TCGGGCTGAT 
TTAAGGGCTG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAT 
AATATTGATG ATGCTTCGCT GCAGGACAAG GAATGCATCT TGGCGTCACT CAACATCAAA 
TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG CTTTACACCC 
CCTGGAATGG ATAGGTCATC TCCAGACAAC AGTCCAGTCC ATGGCCTGTT ACGTCAACCC 
TCCATTACAA CAGGAGCCAA CATCCCTATT ATTACAGAGC TAGCTAAGCC TGGCAAACTT 
CTGCCTTTGG TTTCAATCAG TCAGGAAAAA AACAGTGGAA CCCACATTCT TATGATAACT 
GAACTGGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAACAG AAAAGAAATG GTTTACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807044.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662533.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OLe52817
Clone ID Related Accession (Same CDS sequence)	XM_017807044.1
Accession Version	XM_017807044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGCTAAGC CTGGCAAACT TCTGCCTTTG 
GTTTCAATCA GTCAGGAAAA AAACAGTGGA ACCCACATTC TTATGATAAC TGAACTGGTG 
AATGATTCAA ATGTTCAGTT CTTGGACCAA GATGATGATG ATGATCCAGA CACAGAACTG 
TATCTCACAC AGCCCTTTGC ATGTGGAACC GCGTTTGCTG TCAGTGTGCT GGACTCTCTC 
ATGAGCGCAA CATACTTCAA TGACAATATC CTCACACTGA TACGGACATT GGTAACAGGA 
GGAGCCACAC CAGAGCTGGA AGCACTGATT GCTGAGGAAA ATGCATTGAG AGGAGGTTAC 
AGCACGCCCC AGACACTTGC AAACAGGGAC AGGTGTCGAG TTGCTCAGCT GGCTTTGTAT 
GATGGGCCGT TTGCAGACCT AGGGGATGGA GGTTGTTATG GAGATCTATT TTGTAAAGCA 
CTGAAAACAT ACAATATGCT TTGCTTTGGA ATTTATCGAT TAAGGGATGC ACATCTAAGC 
ACTCCCAGCC AATGCACTAA ACGTTATGTT ATCACAAACC CACCGTATGA ATTTGAGCTT 
GTGCCGACAG ACTTGATCTT CTGTTTGATG CAATTTGACC ACAATGCTGG CCAGTCCCGA 
GCCAGTCTTT CTCATTCCTC CCATTCCTCC TATTCTTCCA GCAAGAAGAG CTCATCAGTT 
CACTCTATCC CATCAACAGC AAATCGACCG AACCGCACCA AGACCAGGGA TTCCCGGGAA 
AAACAGAACA GAAAAGAAAT GGTTTACAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662533.1
CDS	355..3927
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_017807044.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807044.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGCTAAGC CTGGCAAACT TCTGCCTTTG 
GTTTCAATCA GTCAGGAAAA AAACAGTGGA ACCCACATTC TTATGATAAC TGAACTGGTG 
AATGATTCAA ATGTTCAGTT CTTGGACCAA GATGATGATG ATGATCCAGA CACAGAACTG 
TATCTCACAC AGCCCTTTGC ATGTGGAACC GCGTTTGCTG TCAGTGTGCT GGACTCTCTC 
ATGAGCGCAA CATACTTCAA TGACAATATC CTCACACTGA TACGGACATT GGTAACAGGA 
GGAGCCACAC CAGAGCTGGA AGCACTGATT GCTGAGGAAA ATGCATTGAG AGGAGGTTAC 
AGCACGCCCC AGACACTTGC AAACAGGGAC AGGTGTCGAG TTGCTCAGCT GGCTTTGTAT 
GATGGGCCGT TTGCAGACCT AGGGGATGGA GGTTGTTATG GAGATCTATT TTGTAAAGCA 
CTGAAAACAT ACAATATGCT TTGCTTTGGA ATTTATCGAT TAAGGGATGC ACATCTAAGC 
ACTCCCAGCC AATGCACTAA ACGTTATGTT ATCACAAACC CACCGTATGA ATTTGAGCTT 
GTGCCGACAG ACTTGATCTT CTGTTTGATG CAATTTGACC ACAATGCTGG CCAGTCCCGA 
GCCAGTCTTT CTCATTCCTC CCATTCCTCC TATTCTTCCA GCAAGAAGAG CTCATCAGTT 
CACTCTATCC CATCAACAGC AAATCGACCG AACCGCACCA AGACCAGGGA TTCCCGGGAA 
AAACAGAACA GAAAAGAAAT GGTTTACAGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807045.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662534.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X27, mRNA.

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CloneID	OLe52818
Clone ID Related Accession (Same CDS sequence)	XM_017807045.1
Accession Version	XM_017807045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGCTAAGC CTGGCAAACT TCTGCCTTTG 
GTTTCAATCA GTCAGGAAAA AAACAGTGGA ACCCACATTC TTATGATAAC TGAACTGGTG 
AATGATTCAA ATGTTCAGTT CTTGGACCAA GATGATGATG ATGATCCAGA CACAGAACTG 
TATCTCACAC AGCCCTTTGC ATGTGGAACC GCGTTTGCTG TCAGTGTGCT GGACTCTCTC 
ATGAGCGCAA CATACTTCAA TGACAATATC CTCACACTGA TACGGACATT GGTAACAGGA 
GGAGCCACAC CAGAGCTGGA AGCACTGATT GCTGAGGAAA ATGCATTGAG AGGAGGTTAC 
AGCACGCCCC AGACACTTGC AAACAGGGAC AGGTGTCGAG TTGCTCAGCT GGCTTTGTAT 
GATGGGCCGT TTGCAGACCT AGGGGATGGA GGTTGTTATG GAGATCTATT TTGTAAAGCA 
CTGAAAACAT ACAATATGCT TTGCTTTGGA ATTTATCGAT TAAGGGATGC ACATCTAAGC 
ACTCCCAGCC AATGCACTAA ACGTTATGTT ATCACAAACC CACCGTATGA ATTTGAGCTT 
GTGCCGACAG ACTTGATCTT CTGTTTGATG CAATTTGACC ACAATGCTGG CCAGTCCCGA 
GCCAGTCTTT CTCATTCCTC CCATTCCTCC TATTCTTCCA GCAAGAAGAG CTCATCAGTT 
CACTCTATCC CATCAACAGC AAATCGACCG AACCGCACCA AGACCAGGGA TTCCCGGGAA 
AAACAGAAGT ATGTGCAGGA AGATAGACTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662534.1
CDS	355..3927
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKYVQEDRL

Target ORF information:

RefSeq Version	XM_017807045.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807045.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGCTAAGC CTGGCAAACT TCTGCCTTTG 
GTTTCAATCA GTCAGGAAAA AAACAGTGGA ACCCACATTC TTATGATAAC TGAACTGGTG 
AATGATTCAA ATGTTCAGTT CTTGGACCAA GATGATGATG ATGATCCAGA CACAGAACTG 
TATCTCACAC AGCCCTTTGC ATGTGGAACC GCGTTTGCTG TCAGTGTGCT GGACTCTCTC 
ATGAGCGCAA CATACTTCAA TGACAATATC CTCACACTGA TACGGACATT GGTAACAGGA 
GGAGCCACAC CAGAGCTGGA AGCACTGATT GCTGAGGAAA ATGCATTGAG AGGAGGTTAC 
AGCACGCCCC AGACACTTGC AAACAGGGAC AGGTGTCGAG TTGCTCAGCT GGCTTTGTAT 
GATGGGCCGT TTGCAGACCT AGGGGATGGA GGTTGTTATG GAGATCTATT TTGTAAAGCA 
CTGAAAACAT ACAATATGCT TTGCTTTGGA ATTTATCGAT TAAGGGATGC ACATCTAAGC 
ACTCCCAGCC AATGCACTAA ACGTTATGTT ATCACAAACC CACCGTATGA ATTTGAGCTT 
GTGCCGACAG ACTTGATCTT CTGTTTGATG CAATTTGACC ACAATGCTGG CCAGTCCCGA 
GCCAGTCTTT CTCATTCCTC CCATTCCTCC TATTCTTCCA GCAAGAAGAG CTCATCAGTT 
CACTCTATCC CATCAACAGC AAATCGACCG AACCGCACCA AGACCAGGGA TTCCCGGGAA 
AAACAGAAGT ATGTGCAGGA AGATAGACTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807046.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662535.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X28, mRNA.

Price & Availability↑

CloneID	OLe52819
Clone ID Related Accession (Same CDS sequence)	XM_017807046.1
Accession Version	XM_017807046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTTA TGATAACTGA ACTGGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAAGTATG TGCAGGAAGA TAGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662535.1
CDS	355..3924
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKYVQEDRL

Target ORF information:

RefSeq Version	XM_017807046.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X28, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807046.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTTA TGATAACTGA ACTGGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAAGTATG TGCAGGAAGA TAGACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807047.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662536.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X29, mRNA.

Price & Availability↑

CloneID	OLe52820
Clone ID Related Accession (Same CDS sequence)	XM_017807047.1
Accession Version	XM_017807047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X29
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGCTAAGC CTGGCAAACT TCTGCCTTTG 
GTTTCAATCA GTCAGGAAAA AAACAGTGGA ACCCACATTC TTATGATAAC TGAACTGGTG 
AATGATTCAA ATGTTCAGTT CTTGGACCAA GATGATGATG ATGATCCAGA CACAGAACTG 
TATCTCACAC AGCCCTTTGC ATGTGGAACC GCGTTTGCTG TCAGTGTGCT GGACTCTCTC 
ATGAGCGCAA CATACTTCAA TGACAATATC CTCACACTGA TACGGACATT GGTAACAGGA 
GGAGCCACAC CAGAGCTGGA AGCACTGATT GCTGAGGAAA ATGCATTGAG AGGAGGTTAC 
AGCACGCCCC AGACACTTGC AAACAGGGAC AGGTGTCGAG TTGCTCAGCT GGCTTTGTAT 
GATGGGCCGT TTGCAGACCT AGGGGATGGA GGTTGTTATG GAGATCTATT TTGTAAAGCA 
CTGAAAACAT ACAATATGCT TTGCTTTGGA ATTTATCGAT TAAGGGATGC ACATCTAAGC 
ACTCCCAGCC AATGCACTAA ACGTTATGTT ATCACAAACC CACCGTATGA ATTTGAGCTT 
GTGCCGACAG ACTTGATCTT CTGTTTGATG CAATTTGACC ACAATGCTGG CCAGTCCCGA 
GCCAGTCTTT CTCATTCCTC CCATTCCTCC TATTCTTCCA GCAAGAAGAG CTCATCAGTT 
CACTCTATCC CATCAACAGC AAATCGACCG AACCGCACCA AGACCAGGGA TTCCCGGGAA 
AAACAGAAAA AAGAAATGGT TTACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662536.1
CDS	355..3924
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017807047.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X29, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807047.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGCTAAGC CTGGCAAACT TCTGCCTTTG 
GTTTCAATCA GTCAGGAAAA AAACAGTGGA ACCCACATTC TTATGATAAC TGAACTGGTG 
AATGATTCAA ATGTTCAGTT CTTGGACCAA GATGATGATG ATGATCCAGA CACAGAACTG 
TATCTCACAC AGCCCTTTGC ATGTGGAACC GCGTTTGCTG TCAGTGTGCT GGACTCTCTC 
ATGAGCGCAA CATACTTCAA TGACAATATC CTCACACTGA TACGGACATT GGTAACAGGA 
GGAGCCACAC CAGAGCTGGA AGCACTGATT GCTGAGGAAA ATGCATTGAG AGGAGGTTAC 
AGCACGCCCC AGACACTTGC AAACAGGGAC AGGTGTCGAG TTGCTCAGCT GGCTTTGTAT 
GATGGGCCGT TTGCAGACCT AGGGGATGGA GGTTGTTATG GAGATCTATT TTGTAAAGCA 
CTGAAAACAT ACAATATGCT TTGCTTTGGA ATTTATCGAT TAAGGGATGC ACATCTAAGC 
ACTCCCAGCC AATGCACTAA ACGTTATGTT ATCACAAACC CACCGTATGA ATTTGAGCTT 
GTGCCGACAG ACTTGATCTT CTGTTTGATG CAATTTGACC ACAATGCTGG CCAGTCCCGA 
GCCAGTCTTT CTCATTCCTC CCATTCCTCC TATTCTTCCA GCAAGAAGAG CTCATCAGTT 
CACTCTATCC CATCAACAGC AAATCGACCG AACCGCACCA AGACCAGGGA TTCCCGGGAA 
AAACAGAAAA AAGAAATGGT TTACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807048.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662537.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X30, mRNA.

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CloneID	OLe52821
Clone ID Related Accession (Same CDS sequence)	XM_017807048.1
Accession Version	XM_017807048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X30
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTTA TGATAACTGA ACTGGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAAAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662537.1
CDS	355..3921
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELAKPGKLLPLVSISQEKNSGTHILMITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017807048.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807048.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GCTAAGCCTG GCAAACTTCT GCCTTTGGTT 
TCAATCAGTC AGGAAAAAAA CAGTGGAACC CACATTCTTA TGATAACTGA ACTGGTGAAT 
GATTCAAATG TTCAGTTCTT GGACCAAGAT GATGATGATG ATCCAGACAC AGAACTGTAT 
CTCACACAGC CCTTTGCATG TGGAACCGCG TTTGCTGTCA GTGTGCTGGA CTCTCTCATG 
AGCGCAACAT ACTTCAATGA CAATATCCTC ACACTGATAC GGACATTGGT AACAGGAGGA 
GCCACACCAG AGCTGGAAGC ACTGATTGCT GAGGAAAATG CATTGAGAGG AGGTTACAGC 
ACGCCCCAGA CACTTGCAAA CAGGGACAGG TGTCGAGTTG CTCAGCTGGC TTTGTATGAT 
GGGCCGTTTG CAGACCTAGG GGATGGAGGT TGTTATGGAG ATCTATTTTG TAAAGCACTG 
AAAACATACA ATATGCTTTG CTTTGGAATT TATCGATTAA GGGATGCACA TCTAAGCACT 
CCCAGCCAAT GCACTAAACG TTATGTTATC ACAAACCCAC CGTATGAATT TGAGCTTGTG 
CCGACAGACT TGATCTTCTG TTTGATGCAA TTTGACCACA ATGCTGGCCA GTCCCGAGCC 
AGTCTTTCTC ATTCCTCCCA TTCCTCCTAT TCTTCCAGCA AGAAGAGCTC ATCAGTTCAC 
TCTATCCCAT CAACAGCAAA TCGACCGAAC CGCACCAAGA CCAGGGATTC CCGGGAAAAA 
CAGAAAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807049.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662538.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X31, mRNA.

Price & Availability↑

CloneID	OLe52822
Clone ID Related Accession (Same CDS sequence)	XM_017807049.1
Accession Version	XM_017807049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X31
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAACAG AAAAGAAATG GTTTACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662538.1
CDS	355..3876
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_017807049.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807049.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAACAG AAAAGAAATG GTTTACAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807050.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662539.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X32, mRNA.

Price & Availability↑

CloneID	OLe52823
Clone ID Related Accession (Same CDS sequence)	XM_017807050.1
Accession Version	XM_017807050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X32
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAAAAA AGAAATGGTT TACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662539.1
CDS	355..3873
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSKSRTADSLIPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017807050.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807050.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AAGAGCCGTA CAGCAGACTC ATTGATCCCA ATAGAATCCT GCCAGAATTT CTACAAAGAT 
TTCACATTAC AGATCGACAT GGCTTTCAAT GTGTTCTTCC TACTCTACTT CGGCTTGCGT 
TTTATAGCAG CCAATGACAA GCTATGGTTT TGGCTAGAAG TTAACTCAGT GGTAGATTTC 
TTCACGGTCC CTCCAGTGTT TGTGTCAGTA TATTTAAACA GAAGTTGGCT TGGTTTAAGA 
TTTTTAAGAG CCCTTAGACT GATACAGTTT TCAGAAATTT TGCAGTTTCT GAATATACTT 
AAAACAAGTA ATTCCATCAA GCTAGTGAAT CTGTGCTCTA TATTTATCAG CACGTGGCTG 
ACAGCAGCTG GGTTCATACA TTTGGTGGAG AACTCAGGGG ATCCATGGGA AAACTTCCAA 
AATAATCAAC CGCTAACATA CTGGGAATGT GTTTATTTAC TGATGGTTAC AATGTCCACT 
GTTGGTTATG GAGATGTTTA TGCAAAAACA ACCCTTGGTC GCCTTTTCAT GGTCTTCTTC 
ATTCTTGGGG GATTGGCCAT GTTTGCCAGC TACGTCCCTG AAATCATAGA GTTAATAGGA 
AACCGCAAGA AATATGGTGG TTCCTATAGT GCGGTTAGTG GAAGAAAGCA CATAGTTGTC 
TGTGGTCACA TAACACTTGA AAGCGTGTCC AACTTCCTGA AGGACTTCCT GCACAAGGAT 
AGGGATGATG TCAACGTAGA AATCGTCTTT CTGCATAATA TCTCCCCTAA CCTTGAGCTG 
GAAGCTCTTT TTAAACGACA CTTCACTCAG GTGGAATTTT ATCAGGGTTC AGTCCTTAAT 
CCCCATGACC TGGCGAGAGT GAAGATAGAG TCAGCAGATG CGTGCCTAAT CCTTGCCAAT 
AAATACTGCG CTGACCCAGA TGCTGAAGAT GCCTCCAATA TTATGAGAGT AATTTCAATA 
AAGAATTATC ATCCGAAGAT AAGAATTATC ACTCAGATGT TGCAGTATCA CAATAAGGCC 
CATCTGCTAA ATATCCCAAG TTGGAACTGG AAAGAAGGGG ATGATGCAAT CTGTCTTGCT 
GAGCTCAAGC TGGGTTTCAT AGCCCAGAGC TGCCTGGCAC CAGGCCTTTC AACAATGTTA 
GCCAACCTCT TCTCTATGAG GTCATTCATA AAGATTGAGG AGGATACGTG GCAGAAATAC 
TACCTGGAAG GAGTTGCAAA TGAAATGTAT ACAGAATATC TGTCTAGTGC CTTTGTGGGT 
TTGTCCTTCC CTGCAGTTTG TGAACTGGTG TTTGCGAAAC TAAAGCTCTT AATGATAGCC 
ATAGAATACA AGTCGGAAAA ACGCGAAAGC AGAAGCCGAA AGCGTATATT AATCAATCCT 
GGAAACCACG TCAAGATTCA AGAAGGTACC TTAGGATTCT TCATTGCAAG TGATGCCAAA 
GAAGTAAAAA GGGCATATTT TTACTGCAAG GCCTGTCATG ATGACATCAC GGATCCCAAA 
AGGATAAAAA AATGTGGCTG CAAACGGCTG ATCTATTTTG AAGATGAGCA GCCATCGACA 
CTATCACCCA AAAAAAAGCA GCGAAACGGA GGCATGAGAA ATTCACCCAA CTCCTCACCC 
AAGCTGATGA GGCATGACCC CTTGTTAATT CCTGGCAATG AGCAGATTGA CAATATGGAT 
GCCAACGTGA AAAAATATGA CTCTACAGGG ATGTTTCATT GGTGTCCAGC CAAGGACATT 
GAAAAGGTCA TTTTGACTCG AAGTGAAGCA GCGATGACAG TTTTAAGTGG GCATGTAGTC 
GTTTGCATAT TTGGGGATGT TAAATCTGCT TTGATTGGAT TAAGAAATTT AGTGATGCCA 
TTACGAGCCA GCAACTTTCA CTACCATGAA CTCAAGCACA TTGTGTTTGT GGGTTCACTT 
GAATACCTGA GAAGGGAATG GGAGACACTG CATAACTTCC CCAAGGTTTC AATCTTGCCT 
GGTACGCCAT TAAGTCGGGC TGATTTAAGG GCTGTCAACA TCAACCTCTG CGACATGTGC 
GTTATCCTGT CAGCCAATCA GAATAATATT GATGATGCTT CGCTGCAGGA CAAGGAATGC 
ATCTTGGCGT CACTCAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG 
GCTAATTCCC AAGGCTTTAC ACCCCCTGGA ATGGATAGGT CATCTCCAGA CAACAGTCCA 
GTCCATGGCC TGTTACGTCA ACCCTCCATT ACAACAGGAG CCAACATCCC TATTATTACA 
GAGCTAGTGA ATGATTCAAA TGTTCAGTTC TTGGACCAAG ATGATGATGA TGATCCAGAC 
ACAGAACTGT ATCTCACACA GCCCTTTGCA TGTGGAACCG CGTTTGCTGT CAGTGTGCTG 
GACTCTCTCA TGAGCGCAAC ATACTTCAAT GACAATATCC TCACACTGAT ACGGACATTG 
GTAACAGGAG GAGCCACACC AGAGCTGGAA GCACTGATTG CTGAGGAAAA TGCATTGAGA 
GGAGGTTACA GCACGCCCCA GACACTTGCA AACAGGGACA GGTGTCGAGT TGCTCAGCTG 
GCTTTGTATG ATGGGCCGTT TGCAGACCTA GGGGATGGAG GTTGTTATGG AGATCTATTT 
TGTAAAGCAC TGAAAACATA CAATATGCTT TGCTTTGGAA TTTATCGATT AAGGGATGCA 
CATCTAAGCA CTCCCAGCCA ATGCACTAAA CGTTATGTTA TCACAAACCC ACCGTATGAA 
TTTGAGCTTG TGCCGACAGA CTTGATCTTC TGTTTGATGC AATTTGACCA CAATGCTGGC 
CAGTCCCGAG CCAGTCTTTC TCATTCCTCC CATTCCTCCT ATTCTTCCAG CAAGAAGAGC 
TCATCAGTTC ACTCTATCCC ATCAACAGCA AATCGACCGA ACCGCACCAA GACCAGGGAT 
TCCCGGGAAA AACAGAAAAA AGAAATGGTT TACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807051.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662540.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X33, mRNA.

Price & Availability↑

CloneID	OLe52824
Clone ID Related Accession (Same CDS sequence)	XM_017807051.1
Accession Version	XM_017807051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X33
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGTGAATG ATTCAAATGT TCAGTTCTTG 
GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TCACACAGCC CTTTGCATGT 
GGAACCGCGT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
CTGATTGCTG AGGAAAATGC ATTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
AGGGACAGGT GTCGAGTTGC TCAGCTGGCT TTGTATGATG GGCCGTTTGC AGACCTAGGG 
GATGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
TTTGGAATTT ATCGATTAAG GGATGCACAT CTAAGCACTC CCAGCCAATG CACTAAACGT 
TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
TTGATGCAAT TTGACCACAA TGCTGGCCAG TCCCGAGCCA GTCTTTCTCA TTCCTCCCAT 
TCCTCCTATT CTTCCAGCAA GAAGAGCTCA TCAGTTCACT CTATCCCATC AACAGCAAAT 
CGACCGAACC GCACCAAGAC CAGGGATTCC CGGGAAAAAC AGAACAGAAA AGAAATGGTT 
TACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662540.1
CDS	355..3843
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_017807051.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X33, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807051.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGTGAATG ATTCAAATGT TCAGTTCTTG 
GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TCACACAGCC CTTTGCATGT 
GGAACCGCGT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
CTGATTGCTG AGGAAAATGC ATTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
AGGGACAGGT GTCGAGTTGC TCAGCTGGCT TTGTATGATG GGCCGTTTGC AGACCTAGGG 
GATGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
TTTGGAATTT ATCGATTAAG GGATGCACAT CTAAGCACTC CCAGCCAATG CACTAAACGT 
TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
TTGATGCAAT TTGACCACAA TGCTGGCCAG TCCCGAGCCA GTCTTTCTCA TTCCTCCCAT 
TCCTCCTATT CTTCCAGCAA GAAGAGCTCA TCAGTTCACT CTATCCCATC AACAGCAAAT 
CGACCGAACC GCACCAAGAC CAGGGATTCC CGGGAAAAAC AGAACAGAAA AGAAATGGTT 
TACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807052.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662541.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X34, mRNA.

Price & Availability↑

CloneID	OLe52825
Clone ID Related Accession (Same CDS sequence)	XM_017807052.1
Accession Version	XM_017807052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X34
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA AGTATGTGCA GGAAGATAGA 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662541.1
CDS	355..3840
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKYVQEDRL

Target ORF information:

RefSeq Version	XM_017807052.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807052.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA AGTATGTGCA GGAAGATAGA 
CTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807053.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662542.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X35, mRNA.

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CloneID	OLe52826
Clone ID Related Accession (Same CDS sequence)	XM_017807053.1
Accession Version	XM_017807053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGTGAATG ATTCAAATGT TCAGTTCTTG 
GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TCACACAGCC CTTTGCATGT 
GGAACCGCGT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
CTGATTGCTG AGGAAAATGC ATTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
AGGGACAGGT GTCGAGTTGC TCAGCTGGCT TTGTATGATG GGCCGTTTGC AGACCTAGGG 
GATGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
TTTGGAATTT ATCGATTAAG GGATGCACAT CTAAGCACTC CCAGCCAATG CACTAAACGT 
TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
TTGATGCAAT TTGACCACAA TGCTGGCCAG TCCCGAGCCA GTCTTTCTCA TTCCTCCCAT 
TCCTCCTATT CTTCCAGCAA GAAGAGCTCA TCAGTTCACT CTATCCCATC AACAGCAAAT 
CGACCGAACC GCACCAAGAC CAGGGATTCC CGGGAAAAAC AGAAAAAAGA AATGGTTTAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662542.1
CDS	355..3840
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESRSRKRILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017807053.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X35, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807053.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGAA GCCGAAAGCG TATATTAATC AATCCTGGAA ACCACGTCAA GATTCAAGAA 
GGTACCTTAG GATTCTTCAT TGCAAGTGAT GCCAAAGAAG TAAAAAGGGC ATATTTTTAC 
TGCAAGGCCT GTCATGATGA CATCACGGAT CCCAAAAGGA TAAAAAAATG TGGCTGCAAA 
CGGCTTGAAG ATGAGCAGCC ATCGACACTA TCACCCAAAA AAAAGCAGCG AAACGGAGGC 
ATGAGAAATT CACCCAACTC CTCACCCAAG CTGATGAGGC ATGACCCCTT GTTAATTCCT 
GGCAATGAGC AGATTGACAA TATGGATGCC AACGTGAAAA AATATGACTC TACAGGGATG 
TTTCATTGGT GTCCAGCCAA GGACATTGAA AAGGTCATTT TGACTCGAAG TGAAGCAGCG 
ATGACAGTTT TAAGTGGGCA TGTAGTCGTT TGCATATTTG GGGATGTTAA ATCTGCTTTG 
ATTGGATTAA GAAATTTAGT GATGCCATTA CGAGCCAGCA ACTTTCACTA CCATGAACTC 
AAGCACATTG TGTTTGTGGG TTCACTTGAA TACCTGAGAA GGGAATGGGA GACACTGCAT 
AACTTCCCCA AGGTTTCAAT CTTGCCTGGT ACGCCATTAA GTCGGGCTGA TTTAAGGGCT 
GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA TAATATTGAT 
GATGCTTCGC TGCAGGACAA GGAATGCATC TTGGCGTCAC TCAACATCAA ATCTATGCAG 
TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GCTTTACACC CCCTGGAATG 
GATAGGTCAT CTCCAGACAA CAGTCCAGTC CATGGCCTGT TACGTCAACC CTCCATTACA 
ACAGGAGCCA ACATCCCTAT TATTACAGAG CTAGTGAATG ATTCAAATGT TCAGTTCTTG 
GACCAAGATG ATGATGATGA TCCAGACACA GAACTGTATC TCACACAGCC CTTTGCATGT 
GGAACCGCGT TTGCTGTCAG TGTGCTGGAC TCTCTCATGA GCGCAACATA CTTCAATGAC 
AATATCCTCA CACTGATACG GACATTGGTA ACAGGAGGAG CCACACCAGA GCTGGAAGCA 
CTGATTGCTG AGGAAAATGC ATTGAGAGGA GGTTACAGCA CGCCCCAGAC ACTTGCAAAC 
AGGGACAGGT GTCGAGTTGC TCAGCTGGCT TTGTATGATG GGCCGTTTGC AGACCTAGGG 
GATGGAGGTT GTTATGGAGA TCTATTTTGT AAAGCACTGA AAACATACAA TATGCTTTGC 
TTTGGAATTT ATCGATTAAG GGATGCACAT CTAAGCACTC CCAGCCAATG CACTAAACGT 
TATGTTATCA CAAACCCACC GTATGAATTT GAGCTTGTGC CGACAGACTT GATCTTCTGT 
TTGATGCAAT TTGACCACAA TGCTGGCCAG TCCCGAGCCA GTCTTTCTCA TTCCTCCCAT 
TCCTCCTATT CTTCCAGCAA GAAGAGCTCA TCAGTTCACT CTATCCCATC AACAGCAAAT 
CGACCGAACC GCACCAAGAC CAGGGATTCC CGGGAAAAAC AGAAAAAAGA AATGGTTTAC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807054.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662543.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X36, mRNA.

Price & Availability↑

CloneID	OLe52827
Clone ID Related Accession (Same CDS sequence)	XM_017807054.1
Accession Version	XM_017807054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X36
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA AAAAAGAAAT GGTTTACAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662543.1
CDS	355..3837
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLIYFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017807054.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X36, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807054.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTGATCTAT 
TTTGAAGATG AGCAGCCATC GACACTATCA CCCAAAAAAA AGCAGCGAAA CGGAGGCATG 
AGAAATTCAC CCAACTCCTC ACCCAAGCTG ATGAGGCATG ACCCCTTGTT AATTCCTGGC 
AATGAGCAGA TTGACAATAT GGATGCCAAC GTGAAAAAAT ATGACTCTAC AGGGATGTTT 
CATTGGTGTC CAGCCAAGGA CATTGAAAAG GTCATTTTGA CTCGAAGTGA AGCAGCGATG 
ACAGTTTTAA GTGGGCATGT AGTCGTTTGC ATATTTGGGG ATGTTAAATC TGCTTTGATT 
GGATTAAGAA ATTTAGTGAT GCCATTACGA GCCAGCAACT TTCACTACCA TGAACTCAAG 
CACATTGTGT TTGTGGGTTC ACTTGAATAC CTGAGAAGGG AATGGGAGAC ACTGCATAAC 
TTCCCCAAGG TTTCAATCTT GCCTGGTACG CCATTAAGTC GGGCTGATTT AAGGGCTGTC 
AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAATAA TATTGATGAT 
GCTTCGCTGC AGGACAAGGA ATGCATCTTG GCGTCACTCA ACATCAAATC TATGCAGTTT 
GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGCT TTACACCCCC TGGAATGGAT 
AGGTCATCTC CAGACAACAG TCCAGTCCAT GGCCTGTTAC GTCAACCCTC CATTACAACA 
GGAGCCAACA TCCCTATTAT TACAGAGCTA GTGAATGATT CAAATGTTCA GTTCTTGGAC 
CAAGATGATG ATGATGATCC AGACACAGAA CTGTATCTCA CACAGCCCTT TGCATGTGGA 
ACCGCGTTTG CTGTCAGTGT GCTGGACTCT CTCATGAGCG CAACATACTT CAATGACAAT 
ATCCTCACAC TGATACGGAC ATTGGTAACA GGAGGAGCCA CACCAGAGCT GGAAGCACTG 
ATTGCTGAGG AAAATGCATT GAGAGGAGGT TACAGCACGC CCCAGACACT TGCAAACAGG 
GACAGGTGTC GAGTTGCTCA GCTGGCTTTG TATGATGGGC CGTTTGCAGA CCTAGGGGAT 
GGAGGTTGTT ATGGAGATCT ATTTTGTAAA GCACTGAAAA CATACAATAT GCTTTGCTTT 
GGAATTTATC GATTAAGGGA TGCACATCTA AGCACTCCCA GCCAATGCAC TAAACGTTAT 
GTTATCACAA ACCCACCGTA TGAATTTGAG CTTGTGCCGA CAGACTTGAT CTTCTGTTTG 
ATGCAATTTG ACCACAATGC TGGCCAGTCC CGAGCCAGTC TTTCTCATTC CTCCCATTCC 
TCCTATTCTT CCAGCAAGAA GAGCTCATCA GTTCACTCTA TCCCATCAAC AGCAAATCGA 
CCGAACCGCA CCAAGACCAG GGATTCCCGG GAAAAACAGA AAAAAGAAAT GGTTTACAGA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807055.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662544.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X37, mRNA.

Price & Availability↑

CloneID	OLe52828
Clone ID Related Accession (Same CDS sequence)	XM_017807055.1
Accession Version	XM_017807055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X37
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AAGTATGTGC AGGAAGATAG ACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662544.1
CDS	355..3831
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKYVQEDRL

Target ORF information:

RefSeq Version	XM_017807055.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X37, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807055.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AAGTATGTGC AGGAAGATAG ACTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807056.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662545.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X38, mRNA.

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CloneID	OLe52829
Clone ID Related Accession (Same CDS sequence)	XM_017807056.1
Accession Version	XM_017807056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3477bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X38
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662545.1
CDS	355..3831
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQNRKEMVYR

Target ORF information:

RefSeq Version	XM_017807056.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X38, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807056.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AACAGAAAAG AAATGGTTTA CAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807057.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662546.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X39, mRNA.

Price & Availability↑

CloneID	OLe52830
Clone ID Related Accession (Same CDS sequence)	XM_017807057.1
Accession Version	XM_017807057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X39
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AAAAAAGAAA TGGTTTACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662546.1
CDS	355..3828
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRLEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017807057.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X39, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807057.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCTTGAAGAT 
GAGCAGCCAT CGACACTATC ACCCAAAAAA AAGCAGCGAA ACGGAGGCAT GAGAAATTCA 
CCCAACTCCT CACCCAAGCT GATGAGGCAT GACCCCTTGT TAATTCCTGG CAATGAGCAG 
ATTGACAATA TGGATGCCAA CGTGAAAAAA TATGACTCTA CAGGGATGTT TCATTGGTGT 
CCAGCCAAGG ACATTGAAAA GGTCATTTTG ACTCGAAGTG AAGCAGCGAT GACAGTTTTA 
AGTGGGCATG TAGTCGTTTG CATATTTGGG GATGTTAAAT CTGCTTTGAT TGGATTAAGA 
AATTTAGTGA TGCCATTACG AGCCAGCAAC TTTCACTACC ATGAACTCAA GCACATTGTG 
TTTGTGGGTT CACTTGAATA CCTGAGAAGG GAATGGGAGA CACTGCATAA CTTCCCCAAG 
GTTTCAATCT TGCCTGGTAC GCCATTAAGT CGGGCTGATT TAAGGGCTGT CAACATCAAC 
CTCTGCGACA TGTGCGTTAT CCTGTCAGCC AATCAGAATA ATATTGATGA TGCTTCGCTG 
CAGGACAAGG AATGCATCTT GGCGTCACTC AACATCAAAT CTATGCAGTT TGATGACAGC 
ATTGGGGTCT TGCAGGCTAA TTCCCAAGGC TTTACACCCC CTGGAATGGA TAGGTCATCT 
CCAGACAACA GTCCAGTCCA TGGCCTGTTA CGTCAACCCT CCATTACAAC AGGAGCCAAC 
ATCCCTATTA TTACAGAGCT AGTGAATGAT TCAAATGTTC AGTTCTTGGA CCAAGATGAT 
GATGATGATC CAGACACAGA ACTGTATCTC ACACAGCCCT TTGCATGTGG AACCGCGTTT 
GCTGTCAGTG TGCTGGACTC TCTCATGAGC GCAACATACT TCAATGACAA TATCCTCACA 
CTGATACGGA CATTGGTAAC AGGAGGAGCC ACACCAGAGC TGGAAGCACT GATTGCTGAG 
GAAAATGCAT TGAGAGGAGG TTACAGCACG CCCCAGACAC TTGCAAACAG GGACAGGTGT 
CGAGTTGCTC AGCTGGCTTT GTATGATGGG CCGTTTGCAG ACCTAGGGGA TGGAGGTTGT 
TATGGAGATC TATTTTGTAA AGCACTGAAA ACATACAATA TGCTTTGCTT TGGAATTTAT 
CGATTAAGGG ATGCACATCT AAGCACTCCC AGCCAATGCA CTAAACGTTA TGTTATCACA 
AACCCACCGT ATGAATTTGA GCTTGTGCCG ACAGACTTGA TCTTCTGTTT GATGCAATTT 
GACCACAATG CTGGCCAGTC CCGAGCCAGT CTTTCTCATT CCTCCCATTC CTCCTATTCT 
TCCAGCAAGA AGAGCTCATC AGTTCACTCT ATCCCATCAA CAGCAAATCG ACCGAACCGC 
ACCAAGACCA GGGATTCCCG GGAAAAACAG AAAAAAGAAA TGGTTTACAG ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807058.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662547.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X40, mRNA.

Price & Availability↑

CloneID	OLe52831
Clone ID Related Accession (Same CDS sequence)	XM_017807058.1
Accession Version	XM_017807058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X40
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCCCAAGATG 
TCCATCTACA AGAGAATGAA ACTGGCATGT TGTTTTGATT GCGGACGCTC TGAGCGTGAC 
TGCTCTTGCA TGTCAGGCAG TGTACATAGT AACATGGACA CCCTTGAGAG AGCCTTCCCA 
CTTTCTTCTG TCTCTGTTAA TGATTGCTCC ACCAGTTTAC GTGCCTTTGA AGATGAGCAG 
CCATCGACAC TATCACCCAA AAAAAAGCAG CGAAACGGAG GCATGAGAAA TTCACCCAAC 
TCCTCACCCA AGCTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA GCAGATTGAC 
AATATGGATG CCAACGTGAA AAAATATGAC TCTACAGGGA TGTTTCATTG GTGTCCAGCC 
AAGGACATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG 
CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA 
GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG 
GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACACTGC ATAACTTCCC CAAGGTTTCA 
ATCTTGCCTG GTACGCCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC 
GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC 
AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG 
GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGAA TGGATAGGTC ATCTCCAGAC 
AACAGTCCAG TCCATGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT 
ATTATTACAG AGCTAGTGAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT 
GATCCAGACA CAGAACTGTA TCTCACACAG CCCTTTGCAT GTGGAACCGC GTTTGCTGTC 
AGTGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA 
CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC TGAGGAAAAT 
GCATTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT 
GCTCAGCTGG CTTTGTATGA TGGGCCGTTT GCAGACCTAG GGGATGGAGG TTGTTATGGA 
GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA 
AGGGATGCAC ATCTAAGCAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA 
CCGTATGAAT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTTGACCAC 
AATGCTGGCC AGTCCCGAGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC 
AAGAAGAGCT CATCAGTTCA CTCTATCCCA TCAACAGCAA ATCGACCGAA CCGCACCAAG 
ACCAGGGATT CCCGGGAAAA ACAGAAGTAT GTGCAGGAAG ATAGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662547.1
CDS	355..4005
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRPKMSIYKRMKLACCFDCGRSERDCSCMSGSVHSNMDTLERAFPLSSVSVNDCSTSLRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKYVQEDRL

Target ORF information:

RefSeq Version	XM_017807058.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X40, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807058.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCCCAAGATG 
TCCATCTACA AGAGAATGAA ACTGGCATGT TGTTTTGATT GCGGACGCTC TGAGCGTGAC 
TGCTCTTGCA TGTCAGGCAG TGTACATAGT AACATGGACA CCCTTGAGAG AGCCTTCCCA 
CTTTCTTCTG TCTCTGTTAA TGATTGCTCC ACCAGTTTAC GTGCCTTTGA AGATGAGCAG 
CCATCGACAC TATCACCCAA AAAAAAGCAG CGAAACGGAG GCATGAGAAA TTCACCCAAC 
TCCTCACCCA AGCTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA GCAGATTGAC 
AATATGGATG CCAACGTGAA AAAATATGAC TCTACAGGGA TGTTTCATTG GTGTCCAGCC 
AAGGACATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG 
CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA 
GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG 
GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACACTGC ATAACTTCCC CAAGGTTTCA 
ATCTTGCCTG GTACGCCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC 
GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC 
AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG 
GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGAA TGGATAGGTC ATCTCCAGAC 
AACAGTCCAG TCCATGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT 
ATTATTACAG AGCTAGTGAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT 
GATCCAGACA CAGAACTGTA TCTCACACAG CCCTTTGCAT GTGGAACCGC GTTTGCTGTC 
AGTGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA 
CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC TGAGGAAAAT 
GCATTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT 
GCTCAGCTGG CTTTGTATGA TGGGCCGTTT GCAGACCTAG GGGATGGAGG TTGTTATGGA 
GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA 
AGGGATGCAC ATCTAAGCAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA 
CCGTATGAAT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTTGACCAC 
AATGCTGGCC AGTCCCGAGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC 
AAGAAGAGCT CATCAGTTCA CTCTATCCCA TCAACAGCAA ATCGACCGAA CCGCACCAAG 
ACCAGGGATT CCCGGGAAAA ACAGAAGTAT GTGCAGGAAG ATAGACTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMA1 ( XM_017807059.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017662548.1 Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X41, mRNA.

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CloneID	OLe52832
Clone ID Related Accession (Same CDS sequence)	XM_017807059.1
Accession Version	XM_017807059.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	calcium-activated potassium channel subunit alpha-1 isoform X41
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690197.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCCCAAGATG 
TCCATCTACA AGAGAATGAA ACTGGCATGT TGTTTTGATT GCGGACGCTC TGAGCGTGAC 
TGCTCTTGCA TGTCAGGCAG TGTACATAGT AACATGGACA CCCTTGAGAG AGCCTTCCCA 
CTTTCTTCTG TCTCTGTTAA TGATTGCTCC ACCAGTTTAC GTGCCTTTGA AGATGAGCAG 
CCATCGACAC TATCACCCAA AAAAAAGCAG CGAAACGGAG GCATGAGAAA TTCACCCAAC 
TCCTCACCCA AGCTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA GCAGATTGAC 
AATATGGATG CCAACGTGAA AAAATATGAC TCTACAGGGA TGTTTCATTG GTGTCCAGCC 
AAGGACATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG 
CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA 
GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG 
GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACACTGC ATAACTTCCC CAAGGTTTCA 
ATCTTGCCTG GTACGCCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC 
GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC 
AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG 
GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGAA TGGATAGGTC ATCTCCAGAC 
AACAGTCCAG TCCATGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT 
ATTATTACAG AGCTAGTGAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT 
GATCCAGACA CAGAACTGTA TCTCACACAG CCCTTTGCAT GTGGAACCGC GTTTGCTGTC 
AGTGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA 
CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC TGAGGAAAAT 
GCATTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT 
GCTCAGCTGG CTTTGTATGA TGGGCCGTTT GCAGACCTAG GGGATGGAGG TTGTTATGGA 
GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA 
AGGGATGCAC ATCTAAGCAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA 
CCGTATGAAT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTTGACCAC 
AATGCTGGCC AGTCCCGAGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC 
AAGAAGAGCT CATCAGTTCA CTCTATCCCA TCAACAGCAA ATCGACCGAA CCGCACCAAG 
ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017662548.1
CDS	355..4002
Translation	MANGSGGGSYPGGSGGGGGIRMSNNINANNLNTDSSSSPVNVPKMDALIIPVTMEVPCDSRGQRMWWAFLASSMVTFFGGLFIILLWRTLKYLWTVCCHCGVKNKEAQKINGGGDTQADGACKPTDEKEENVAAEVGWMTSVKDWAGVMISAQTLTGRVLVVLVFALSIGALVIYFIDSSNPIESCQNFYKDFTLQIDMAFNVFFLLYFGLRFIAANDKLWFWLEVNSVVDFFTVPPVFVSVYLNRSWLGLRFLRALRLIQFSEILQFLNILKTSNSIKLVNLCSIFISTWLTAAGFIHLVENSGDPWENFQNNQPLTYWECVYLLMVTMSTVGYGDVYAKTTLGRLFMVFFILGGLAMFASYVPEIIELIGNRKKYGGSYSAVSGRKHIVVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIAQSCLAPGLSTMLANLFSMRSFIKIEEDTWQKYYLEGVANEMYTEYLSSAFVGLSFPAVCELVFAKLKLLMIAIEYKSEKRESSILINPGNHVKIQEGTLGFFIASDAKEVKRAYFYCKACHDDITDPKRIKKCGCKRPKMSIYKRMKLACCFDCGRSERDCSCMSGSVHSNMDTLERAFPLSSVSVNDCSTSLRAFEDEQPSTLSPKKKQRNGGMRNSPNSSPKLMRHDPLLIPGNEQIDNMDANVKKYDSTGMFHWCPAKDIEKVILTRSEAAMTVLSGHVVVCIFGDVKSALIGLRNLVMPLRASNFHYHELKHIVFVGSLEYLRREWETLHNFPKVSILPGTPLSRADLRAVNINLCDMCVILSANQNNIDDASLQDKECILASLNIKSMQFDDSIGVLQANSQGFTPPGMDRSSPDNSPVHGLLRQPSITTGANIPIITELVNDSNVQFLDQDDDDDPDTELYLTQPFACGTAFAVSVLDSLMSATYFNDNILTLIRTLVTGGATPELEALIAEENALRGGYSTPQTLANRDRCRVAQLALYDGPFADLGDGGCYGDLFCKALKTYNMLCFGIYRLRDAHLSTPSQCTKRYVITNPPYEFELVPTDLIFCLMQFDHNAGQSRASLSHSSHSSYSSSKKSSSVHSIPSTANRPNRTKTRDSREKQKKEMVYR

Target ORF information:

RefSeq Version	XM_017807059.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata potassium calcium-activated channel subfamily M alpha 1 (KCNMA1), transcript variant X41, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017807059.1

ORF Insert Sequence:

ATGGCAAATG GCAGCGGCGG CGGCTCCTAC CCGGGCGGCA GCGGCGGCGG CGGCGGCATT 
AGAATGAGTA ACAATATCAA CGCCAACAAT CTCAACACAG ACTCGTCCTC CTCCCCCGTC 
AATGTGCCCA AGATGGATGC GCTCATCATC CCGGTGACCA TGGAGGTGCC CTGCGATAGC 
CGCGGACAGC GCATGTGGTG GGCTTTCCTC GCCTCATCCA TGGTTACTTT CTTTGGGGGA 
CTGTTTATCA TCCTGCTGTG GAGGACCCTC AAGTACCTGT GGACTGTCTG CTGCCACTGC 
GGGGTGAAAA ACAAGGAGGC CCAGAAGATC AATGGTGGTG GAGACACACA GGCAGATGGA 
GCCTGCAAAC CAACAGATGA AAAAGAGGAG AATGTGGCAG CAGAAGTGGG ATGGATGACG 
TCTGTGAAGG ATTGGGCAGG AGTCATGATA TCTGCCCAGA CATTAACTGG CAGAGTACTT 
GTTGTCTTAG TCTTCGCTCT TAGTATTGGT GCTCTTGTAA TATACTTCAT AGATTCATCA 
AACCCAATAG AATCCTGCCA GAATTTCTAC AAAGATTTCA CATTACAGAT CGACATGGCT 
TTCAATGTGT TCTTCCTACT CTACTTCGGC TTGCGTTTTA TAGCAGCCAA TGACAAGCTA 
TGGTTTTGGC TAGAAGTTAA CTCAGTGGTA GATTTCTTCA CGGTCCCTCC AGTGTTTGTG 
TCAGTATATT TAAACAGAAG TTGGCTTGGT TTAAGATTTT TAAGAGCCCT TAGACTGATA 
CAGTTTTCAG AAATTTTGCA GTTTCTGAAT ATACTTAAAA CAAGTAATTC CATCAAGCTA 
GTGAATCTGT GCTCTATATT TATCAGCACG TGGCTGACAG CAGCTGGGTT CATACATTTG 
GTGGAGAACT CAGGGGATCC ATGGGAAAAC TTCCAAAATA ATCAACCGCT AACATACTGG 
GAATGTGTTT ATTTACTGAT GGTTACAATG TCCACTGTTG GTTATGGAGA TGTTTATGCA 
AAAACAACCC TTGGTCGCCT TTTCATGGTC TTCTTCATTC TTGGGGGATT GGCCATGTTT 
GCCAGCTACG TCCCTGAAAT CATAGAGTTA ATAGGAAACC GCAAGAAATA TGGTGGTTCC 
TATAGTGCGG TTAGTGGAAG AAAGCACATA GTTGTCTGTG GTCACATAAC ACTTGAAAGC 
GTGTCCAACT TCCTGAAGGA CTTCCTGCAC AAGGATAGGG ATGATGTCAA CGTAGAAATC 
GTCTTTCTGC ATAATATCTC CCCTAACCTT GAGCTGGAAG CTCTTTTTAA ACGACACTTC 
ACTCAGGTGG AATTTTATCA GGGTTCAGTC CTTAATCCCC ATGACCTGGC GAGAGTGAAG 
ATAGAGTCAG CAGATGCGTG CCTAATCCTT GCCAATAAAT ACTGCGCTGA CCCAGATGCT 
GAAGATGCCT CCAATATTAT GAGAGTAATT TCAATAAAGA ATTATCATCC GAAGATAAGA 
ATTATCACTC AGATGTTGCA GTATCACAAT AAGGCCCATC TGCTAAATAT CCCAAGTTGG 
AACTGGAAAG AAGGGGATGA TGCAATCTGT CTTGCTGAGC TCAAGCTGGG TTTCATAGCC 
CAGAGCTGCC TGGCACCAGG CCTTTCAACA ATGTTAGCCA ACCTCTTCTC TATGAGGTCA 
TTCATAAAGA TTGAGGAGGA TACGTGGCAG AAATACTACC TGGAAGGAGT TGCAAATGAA 
ATGTATACAG AATATCTGTC TAGTGCCTTT GTGGGTTTGT CCTTCCCTGC AGTTTGTGAA 
CTGGTGTTTG CGAAACTAAA GCTCTTAATG ATAGCCATAG AATACAAGTC GGAAAAACGC 
GAAAGCAGTA TATTAATCAA TCCTGGAAAC CACGTCAAGA TTCAAGAAGG TACCTTAGGA 
TTCTTCATTG CAAGTGATGC CAAAGAAGTA AAAAGGGCAT ATTTTTACTG CAAGGCCTGT 
CATGATGACA TCACGGATCC CAAAAGGATA AAAAAATGTG GCTGCAAACG GCCCAAGATG 
TCCATCTACA AGAGAATGAA ACTGGCATGT TGTTTTGATT GCGGACGCTC TGAGCGTGAC 
TGCTCTTGCA TGTCAGGCAG TGTACATAGT AACATGGACA CCCTTGAGAG AGCCTTCCCA 
CTTTCTTCTG TCTCTGTTAA TGATTGCTCC ACCAGTTTAC GTGCCTTTGA AGATGAGCAG 
CCATCGACAC TATCACCCAA AAAAAAGCAG CGAAACGGAG GCATGAGAAA TTCACCCAAC 
TCCTCACCCA AGCTGATGAG GCATGACCCC TTGTTAATTC CTGGCAATGA GCAGATTGAC 
AATATGGATG CCAACGTGAA AAAATATGAC TCTACAGGGA TGTTTCATTG GTGTCCAGCC 
AAGGACATTG AAAAGGTCAT TTTGACTCGA AGTGAAGCAG CGATGACAGT TTTAAGTGGG 
CATGTAGTCG TTTGCATATT TGGGGATGTT AAATCTGCTT TGATTGGATT AAGAAATTTA 
GTGATGCCAT TACGAGCCAG CAACTTTCAC TACCATGAAC TCAAGCACAT TGTGTTTGTG 
GGTTCACTTG AATACCTGAG AAGGGAATGG GAGACACTGC ATAACTTCCC CAAGGTTTCA 
ATCTTGCCTG GTACGCCATT AAGTCGGGCT GATTTAAGGG CTGTCAACAT CAACCTCTGC 
GACATGTGCG TTATCCTGTC AGCCAATCAG AATAATATTG ATGATGCTTC GCTGCAGGAC 
AAGGAATGCA TCTTGGCGTC ACTCAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG 
GTCTTGCAGG CTAATTCCCA AGGCTTTACA CCCCCTGGAA TGGATAGGTC ATCTCCAGAC 
AACAGTCCAG TCCATGGCCT GTTACGTCAA CCCTCCATTA CAACAGGAGC CAACATCCCT 
ATTATTACAG AGCTAGTGAA TGATTCAAAT GTTCAGTTCT TGGACCAAGA TGATGATGAT 
GATCCAGACA CAGAACTGTA TCTCACACAG CCCTTTGCAT GTGGAACCGC GTTTGCTGTC 
AGTGTGCTGG ACTCTCTCAT GAGCGCAACA TACTTCAATG ACAATATCCT CACACTGATA 
CGGACATTGG TAACAGGAGG AGCCACACCA GAGCTGGAAG CACTGATTGC TGAGGAAAAT 
GCATTGAGAG GAGGTTACAG CACGCCCCAG ACACTTGCAA ACAGGGACAG GTGTCGAGTT 
GCTCAGCTGG CTTTGTATGA TGGGCCGTTT GCAGACCTAG GGGATGGAGG TTGTTATGGA 
GATCTATTTT GTAAAGCACT GAAAACATAC AATATGCTTT GCTTTGGAAT TTATCGATTA 
AGGGATGCAC ATCTAAGCAC TCCCAGCCAA TGCACTAAAC GTTATGTTAT CACAAACCCA 
CCGTATGAAT TTGAGCTTGT GCCGACAGAC TTGATCTTCT GTTTGATGCA ATTTGACCAC 
AATGCTGGCC AGTCCCGAGC CAGTCTTTCT CATTCCTCCC ATTCCTCCTA TTCTTCCAGC 
AAGAAGAGCT CATCAGTTCA CTCTATCCCA TCAACAGCAA ATCGACCGAA CCGCACCAAG 
ACCAGGGATT CCCGGGAAAA ACAGAAAAAA GAAATGGTTT ACAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

KCNMA1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: