AGL cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene
Clones
gRNAs

Summary

Gene Symbol	AGL
Entrez Gene ID	102354072
Full Name	amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase
Gene Type	protein-coding
Organism	Latimeria chalumnae(coelacanth)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014490776.1	XP_014346262.1	glycogen debranching enzyme isoform X2
XM_006000008.2	XP_006000070.1	glycogen debranching enzyme isoform X1
XM_006000007.2	XP_006000069.1	glycogen debranching enzyme isoform X1
XM_006000010.2	XP_006000072.1	glycogen debranching enzyme isoform X2
XM_006000005.2	XP_006000067.1	glycogen debranching enzyme isoform X1
XM_006000006.2	XP_006000068.1	glycogen debranching enzyme isoform X1
XM_006000009.2	XP_006000071.1	glycogen debranching enzyme isoform X1

Pathways from BioSystems
Gene Ontology

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa30929	XM_006000007.2 Latest version!	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa30929	XM_006000008.2 Latest version!	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa30929	XM_006000005.2 Latest version!	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa30929	XM_006000006.2 Latest version!	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa30929	XM_006000009.2 Latest version!	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa30930	XM_014490776.1 Latest version!	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OLa30930	XM_006000010.2 Latest version!	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AGL ( XM_006000008.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_006000070.1 Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OLa30929
Clone ID Related Accession (Same CDS sequence)	XM_006000008.2 , XM_006000007.2 , XM_006000005.2 , XM_006000006.2 , XM_006000009.2
Accession Version	XM_006000008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819578.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_006000008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006000070.1
CDS	224..4825
Translation	MMQNKQIRVLLLNDMEKLEKTVFKLEQGYELQFRLGPTLQGKAVTVTTNYPAPKEPFNRYKFRSLEWNNPTGREDDSDKYCKLDLQIAGSFQYFFGYGNDSKSGGGYIVVDPILCVGDNQVLPLDCITLVTYLAKCMGPFDEWEDRLKVAKESGYNMIHFTPLQTLGLSRSCYSLSDQLELNPDFSRSNEKYTWSDVGKLVEKLKKEWNVLCITDVVYNHTAANSKWIQEHPECGYNLVNSPHLKPAWIMDRALWHLTCDIADGKYIHKGLPALIENEQQLNNIRKVLWEDIYPKIKLWEFYQVDIDKAVQQFGTLLSQEKMMTKTKSDPKQHLKIIQDPEYKRFGCTVDMDVALESFIPHNNMPPAYVECCNWFRKRLEELNGERFREVNYHQEQAVNCILGNVLYERLAGHGPKLGPITRKHPLVTKYFAYRFQEMTLEQEELMMHQSNKACHFLAHNGWVMADDPLRNFAESDSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITAKHFYGVRLDNCHSTPLHVAEEMLAAARSVRPNLYVIAELFTGSEFLDNVFVNRLGITSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPCLRPLVPTIAHALFMDVTHDNECPILHRSAYDSLSSSAIVSLASCATGSTRGYDEHVPHQISVVSEERYYSKWNPAADPSNPGEVNFQSGIIAGKRALNRLHQELGAKGFIQVYVDQVDEDIVAVTRHCPSTHQSVVAVSRTAFRDPKASYYRPDVPPMCIPGKIEEVVLEAKTVERNAIPYKKDEKSINGLPNLTVEIREHIQLKDSKIIKQAEVTTKGSNEFVQEIEFESLTPGSVIVFRVSLDPKAQEAVGLLRHHLVQFSSHFKSGSLPDDKAVSILKTPFASIASKLSLADLNYVLYRCDAEEQEDGGGCYNVPNWTPLKYAGLQGLMSVMAEIRPKNDLGHPFCVNLRNGDWMIDYVSNRFIVHSGAPAEVGKWFQAMFTYLKQLPRYLIPCYFDAILTGVYTTLLDTSLKQMSEFIQKGSTFVKHLALGSLQMCGVGRFPALPPVSPSLQDVPYRINDIIKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFISLRGLMLVTGRHLEARNIILAFASTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYSKIVPNGIEILKCPVSRMYPSDSSPPLPAGTVEQPLYDVIQEAMQRHMQGIQYKERNAGPQIDRNMKDEGFNVTVGVDPVTGFVFGGNRFNCGTWMDKMGESERARNLGIPATPRDGSAVEIVGLSKSMVAWLMELHKKNVFPYKGVTVQKNEKSVIITYEEWNRKIQNNFEKLFYVSEDLIDLNEKHPNLIHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPDLFTPSKAWKALDMAEKKLLGPLGMKTLDPDDMVHCGVYDNALDSDNYNVAKGFNYHQGPEWLWPLGYFLRAKLYFSKLMGKETHAETIYLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATMLEVLYDL

Target ORF information:

RefSeq Version	XM_006000008.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006000008.2

ORF Insert Sequence:

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_006000007.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_006000069.1 Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OLa30929
Clone ID Related Accession (Same CDS sequence)	XM_006000008.2 , XM_006000007.2 , XM_006000005.2 , XM_006000006.2 , XM_006000009.2
Accession Version	XM_006000007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819578.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_006000007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006000069.1
CDS	367..4968
Translation	MMQNKQIRVLLLNDMEKLEKTVFKLEQGYELQFRLGPTLQGKAVTVTTNYPAPKEPFNRYKFRSLEWNNPTGREDDSDKYCKLDLQIAGSFQYFFGYGNDSKSGGGYIVVDPILCVGDNQVLPLDCITLVTYLAKCMGPFDEWEDRLKVAKESGYNMIHFTPLQTLGLSRSCYSLSDQLELNPDFSRSNEKYTWSDVGKLVEKLKKEWNVLCITDVVYNHTAANSKWIQEHPECGYNLVNSPHLKPAWIMDRALWHLTCDIADGKYIHKGLPALIENEQQLNNIRKVLWEDIYPKIKLWEFYQVDIDKAVQQFGTLLSQEKMMTKTKSDPKQHLKIIQDPEYKRFGCTVDMDVALESFIPHNNMPPAYVECCNWFRKRLEELNGERFREVNYHQEQAVNCILGNVLYERLAGHGPKLGPITRKHPLVTKYFAYRFQEMTLEQEELMMHQSNKACHFLAHNGWVMADDPLRNFAESDSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITAKHFYGVRLDNCHSTPLHVAEEMLAAARSVRPNLYVIAELFTGSEFLDNVFVNRLGITSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPCLRPLVPTIAHALFMDVTHDNECPILHRSAYDSLSSSAIVSLASCATGSTRGYDEHVPHQISVVSEERYYSKWNPAADPSNPGEVNFQSGIIAGKRALNRLHQELGAKGFIQVYVDQVDEDIVAVTRHCPSTHQSVVAVSRTAFRDPKASYYRPDVPPMCIPGKIEEVVLEAKTVERNAIPYKKDEKSINGLPNLTVEIREHIQLKDSKIIKQAEVTTKGSNEFVQEIEFESLTPGSVIVFRVSLDPKAQEAVGLLRHHLVQFSSHFKSGSLPDDKAVSILKTPFASIASKLSLADLNYVLYRCDAEEQEDGGGCYNVPNWTPLKYAGLQGLMSVMAEIRPKNDLGHPFCVNLRNGDWMIDYVSNRFIVHSGAPAEVGKWFQAMFTYLKQLPRYLIPCYFDAILTGVYTTLLDTSLKQMSEFIQKGSTFVKHLALGSLQMCGVGRFPALPPVSPSLQDVPYRINDIIKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFISLRGLMLVTGRHLEARNIILAFASTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYSKIVPNGIEILKCPVSRMYPSDSSPPLPAGTVEQPLYDVIQEAMQRHMQGIQYKERNAGPQIDRNMKDEGFNVTVGVDPVTGFVFGGNRFNCGTWMDKMGESERARNLGIPATPRDGSAVEIVGLSKSMVAWLMELHKKNVFPYKGVTVQKNEKSVIITYEEWNRKIQNNFEKLFYVSEDLIDLNEKHPNLIHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPDLFTPSKAWKALDMAEKKLLGPLGMKTLDPDDMVHCGVYDNALDSDNYNVAKGFNYHQGPEWLWPLGYFLRAKLYFSKLMGKETHAETIYLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATMLEVLYDL

Target ORF information:

RefSeq Version	XM_006000007.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006000007.2

ORF Insert Sequence:

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_006000005.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_006000067.1 Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

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CloneID	OLa30929
Clone ID Related Accession (Same CDS sequence)	XM_006000008.2 , XM_006000007.2 , XM_006000005.2 , XM_006000006.2 , XM_006000009.2
Accession Version	XM_006000005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819578.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_006000005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006000067.1
CDS	214..4815
Translation	MMQNKQIRVLLLNDMEKLEKTVFKLEQGYELQFRLGPTLQGKAVTVTTNYPAPKEPFNRYKFRSLEWNNPTGREDDSDKYCKLDLQIAGSFQYFFGYGNDSKSGGGYIVVDPILCVGDNQVLPLDCITLVTYLAKCMGPFDEWEDRLKVAKESGYNMIHFTPLQTLGLSRSCYSLSDQLELNPDFSRSNEKYTWSDVGKLVEKLKKEWNVLCITDVVYNHTAANSKWIQEHPECGYNLVNSPHLKPAWIMDRALWHLTCDIADGKYIHKGLPALIENEQQLNNIRKVLWEDIYPKIKLWEFYQVDIDKAVQQFGTLLSQEKMMTKTKSDPKQHLKIIQDPEYKRFGCTVDMDVALESFIPHNNMPPAYVECCNWFRKRLEELNGERFREVNYHQEQAVNCILGNVLYERLAGHGPKLGPITRKHPLVTKYFAYRFQEMTLEQEELMMHQSNKACHFLAHNGWVMADDPLRNFAESDSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITAKHFYGVRLDNCHSTPLHVAEEMLAAARSVRPNLYVIAELFTGSEFLDNVFVNRLGITSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPCLRPLVPTIAHALFMDVTHDNECPILHRSAYDSLSSSAIVSLASCATGSTRGYDEHVPHQISVVSEERYYSKWNPAADPSNPGEVNFQSGIIAGKRALNRLHQELGAKGFIQVYVDQVDEDIVAVTRHCPSTHQSVVAVSRTAFRDPKASYYRPDVPPMCIPGKIEEVVLEAKTVERNAIPYKKDEKSINGLPNLTVEIREHIQLKDSKIIKQAEVTTKGSNEFVQEIEFESLTPGSVIVFRVSLDPKAQEAVGLLRHHLVQFSSHFKSGSLPDDKAVSILKTPFASIASKLSLADLNYVLYRCDAEEQEDGGGCYNVPNWTPLKYAGLQGLMSVMAEIRPKNDLGHPFCVNLRNGDWMIDYVSNRFIVHSGAPAEVGKWFQAMFTYLKQLPRYLIPCYFDAILTGVYTTLLDTSLKQMSEFIQKGSTFVKHLALGSLQMCGVGRFPALPPVSPSLQDVPYRINDIIKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFISLRGLMLVTGRHLEARNIILAFASTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYSKIVPNGIEILKCPVSRMYPSDSSPPLPAGTVEQPLYDVIQEAMQRHMQGIQYKERNAGPQIDRNMKDEGFNVTVGVDPVTGFVFGGNRFNCGTWMDKMGESERARNLGIPATPRDGSAVEIVGLSKSMVAWLMELHKKNVFPYKGVTVQKNEKSVIITYEEWNRKIQNNFEKLFYVSEDLIDLNEKHPNLIHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPDLFTPSKAWKALDMAEKKLLGPLGMKTLDPDDMVHCGVYDNALDSDNYNVAKGFNYHQGPEWLWPLGYFLRAKLYFSKLMGKETHAETIYLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATMLEVLYDL

Target ORF information:

RefSeq Version	XM_006000005.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006000005.2

ORF Insert Sequence:

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_006000006.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_006000068.1 Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

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CloneID	OLa30929
Clone ID Related Accession (Same CDS sequence)	XM_006000008.2 , XM_006000007.2 , XM_006000005.2 , XM_006000006.2 , XM_006000009.2
Accession Version	XM_006000006.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819578.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_006000006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006000068.1
CDS	253..4854
Translation	MMQNKQIRVLLLNDMEKLEKTVFKLEQGYELQFRLGPTLQGKAVTVTTNYPAPKEPFNRYKFRSLEWNNPTGREDDSDKYCKLDLQIAGSFQYFFGYGNDSKSGGGYIVVDPILCVGDNQVLPLDCITLVTYLAKCMGPFDEWEDRLKVAKESGYNMIHFTPLQTLGLSRSCYSLSDQLELNPDFSRSNEKYTWSDVGKLVEKLKKEWNVLCITDVVYNHTAANSKWIQEHPECGYNLVNSPHLKPAWIMDRALWHLTCDIADGKYIHKGLPALIENEQQLNNIRKVLWEDIYPKIKLWEFYQVDIDKAVQQFGTLLSQEKMMTKTKSDPKQHLKIIQDPEYKRFGCTVDMDVALESFIPHNNMPPAYVECCNWFRKRLEELNGERFREVNYHQEQAVNCILGNVLYERLAGHGPKLGPITRKHPLVTKYFAYRFQEMTLEQEELMMHQSNKACHFLAHNGWVMADDPLRNFAESDSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITAKHFYGVRLDNCHSTPLHVAEEMLAAARSVRPNLYVIAELFTGSEFLDNVFVNRLGITSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPCLRPLVPTIAHALFMDVTHDNECPILHRSAYDSLSSSAIVSLASCATGSTRGYDEHVPHQISVVSEERYYSKWNPAADPSNPGEVNFQSGIIAGKRALNRLHQELGAKGFIQVYVDQVDEDIVAVTRHCPSTHQSVVAVSRTAFRDPKASYYRPDVPPMCIPGKIEEVVLEAKTVERNAIPYKKDEKSINGLPNLTVEIREHIQLKDSKIIKQAEVTTKGSNEFVQEIEFESLTPGSVIVFRVSLDPKAQEAVGLLRHHLVQFSSHFKSGSLPDDKAVSILKTPFASIASKLSLADLNYVLYRCDAEEQEDGGGCYNVPNWTPLKYAGLQGLMSVMAEIRPKNDLGHPFCVNLRNGDWMIDYVSNRFIVHSGAPAEVGKWFQAMFTYLKQLPRYLIPCYFDAILTGVYTTLLDTSLKQMSEFIQKGSTFVKHLALGSLQMCGVGRFPALPPVSPSLQDVPYRINDIIKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFISLRGLMLVTGRHLEARNIILAFASTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYSKIVPNGIEILKCPVSRMYPSDSSPPLPAGTVEQPLYDVIQEAMQRHMQGIQYKERNAGPQIDRNMKDEGFNVTVGVDPVTGFVFGGNRFNCGTWMDKMGESERARNLGIPATPRDGSAVEIVGLSKSMVAWLMELHKKNVFPYKGVTVQKNEKSVIITYEEWNRKIQNNFEKLFYVSEDLIDLNEKHPNLIHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPDLFTPSKAWKALDMAEKKLLGPLGMKTLDPDDMVHCGVYDNALDSDNYNVAKGFNYHQGPEWLWPLGYFLRAKLYFSKLMGKETHAETIYLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATMLEVLYDL

Target ORF information:

RefSeq Version	XM_006000006.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006000006.2

ORF Insert Sequence:

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_006000009.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_006000071.1 Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X5, mRNA.

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CloneID	OLa30929
Clone ID Related Accession (Same CDS sequence)	XM_006000008.2 , XM_006000007.2 , XM_006000005.2 , XM_006000006.2 , XM_006000009.2
Accession Version	XM_006000009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	glycogen debranching enzyme isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819578.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_006000009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006000071.1
CDS	365..4966
Translation	MMQNKQIRVLLLNDMEKLEKTVFKLEQGYELQFRLGPTLQGKAVTVTTNYPAPKEPFNRYKFRSLEWNNPTGREDDSDKYCKLDLQIAGSFQYFFGYGNDSKSGGGYIVVDPILCVGDNQVLPLDCITLVTYLAKCMGPFDEWEDRLKVAKESGYNMIHFTPLQTLGLSRSCYSLSDQLELNPDFSRSNEKYTWSDVGKLVEKLKKEWNVLCITDVVYNHTAANSKWIQEHPECGYNLVNSPHLKPAWIMDRALWHLTCDIADGKYIHKGLPALIENEQQLNNIRKVLWEDIYPKIKLWEFYQVDIDKAVQQFGTLLSQEKMMTKTKSDPKQHLKIIQDPEYKRFGCTVDMDVALESFIPHNNMPPAYVECCNWFRKRLEELNGERFREVNYHQEQAVNCILGNVLYERLAGHGPKLGPITRKHPLVTKYFAYRFQEMTLEQEELMMHQSNKACHFLAHNGWVMADDPLRNFAESDSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITAKHFYGVRLDNCHSTPLHVAEEMLAAARSVRPNLYVIAELFTGSEFLDNVFVNRLGITSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPCLRPLVPTIAHALFMDVTHDNECPILHRSAYDSLSSSAIVSLASCATGSTRGYDEHVPHQISVVSEERYYSKWNPAADPSNPGEVNFQSGIIAGKRALNRLHQELGAKGFIQVYVDQVDEDIVAVTRHCPSTHQSVVAVSRTAFRDPKASYYRPDVPPMCIPGKIEEVVLEAKTVERNAIPYKKDEKSINGLPNLTVEIREHIQLKDSKIIKQAEVTTKGSNEFVQEIEFESLTPGSVIVFRVSLDPKAQEAVGLLRHHLVQFSSHFKSGSLPDDKAVSILKTPFASIASKLSLADLNYVLYRCDAEEQEDGGGCYNVPNWTPLKYAGLQGLMSVMAEIRPKNDLGHPFCVNLRNGDWMIDYVSNRFIVHSGAPAEVGKWFQAMFTYLKQLPRYLIPCYFDAILTGVYTTLLDTSLKQMSEFIQKGSTFVKHLALGSLQMCGVGRFPALPPVSPSLQDVPYRINDIIKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFISLRGLMLVTGRHLEARNIILAFASTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYSKIVPNGIEILKCPVSRMYPSDSSPPLPAGTVEQPLYDVIQEAMQRHMQGIQYKERNAGPQIDRNMKDEGFNVTVGVDPVTGFVFGGNRFNCGTWMDKMGESERARNLGIPATPRDGSAVEIVGLSKSMVAWLMELHKKNVFPYKGVTVQKNEKSVIITYEEWNRKIQNNFEKLFYVSEDLIDLNEKHPNLIHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPDLFTPSKAWKALDMAEKKLLGPLGMKTLDPDDMVHCGVYDNALDSDNYNVAKGFNYHQGPEWLWPLGYFLRAKLYFSKLMGKETHAETIYLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATMLEVLYDL

Target ORF information:

RefSeq Version	XM_006000009.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006000009.2

ORF Insert Sequence:

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGGAGATG 
CTGGCAGCTG CCAGGTCAGT CAGGCCTAAT CTTTATGTGA TAGCAGAACT CTTCACAGGC 
AGCGAGTTCC TCGATAATGT ATTTGTCAAT CGCCTAGGAA TTACAAGTCT GATTAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_014490776.1 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_014346262.1 Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X7, mRNA.

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CloneID	OLa30930
Clone ID Related Accession (Same CDS sequence)	XM_014490776.1 , XM_006000010.2
Accession Version	XM_014490776.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819578.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGTATATG 
CTGGACACCG CCAGGATGTT GAGGCCTAAC TTGTACTTAA TGGCTGAGCT GTTTACAGGA 
AGTGAAGAGC TGGACAATGT CTTTGTAACT AAACTTGGCA TTAGCTCCTT AATAAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014346262.1
CDS	368..4969
Translation	MMQNKQIRVLLLNDMEKLEKTVFKLEQGYELQFRLGPTLQGKAVTVTTNYPAPKEPFNRYKFRSLEWNNPTGREDDSDKYCKLDLQIAGSFQYFFGYGNDSKSGGGYIVVDPILCVGDNQVLPLDCITLVTYLAKCMGPFDEWEDRLKVAKESGYNMIHFTPLQTLGLSRSCYSLSDQLELNPDFSRSNEKYTWSDVGKLVEKLKKEWNVLCITDVVYNHTAANSKWIQEHPECGYNLVNSPHLKPAWIMDRALWHLTCDIADGKYIHKGLPALIENEQQLNNIRKVLWEDIYPKIKLWEFYQVDIDKAVQQFGTLLSQEKMMTKTKSDPKQHLKIIQDPEYKRFGCTVDMDVALESFIPHNNMPPAYVECCNWFRKRLEELNGERFREVNYHQEQAVNCILGNVLYERLAGHGPKLGPITRKHPLVTKYFAYRFQEMTLEQEELMMHQSNKACHFLAHNGWVMADDPLRNFAESDSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITAKHFYGVRLDNCHSTPLHVAEYMLDTARMLRPNLYLMAELFTGSEELDNVFVTKLGISSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPCLRPLVPTIAHALFMDVTHDNECPILHRSAYDSLSSSAIVSLASCATGSTRGYDEHVPHQISVVSEERYYSKWNPAADPSNPGEVNFQSGIIAGKRALNRLHQELGAKGFIQVYVDQVDEDIVAVTRHCPSTHQSVVAVSRTAFRDPKASYYRPDVPPMCIPGKIEEVVLEAKTVERNAIPYKKDEKSINGLPNLTVEIREHIQLKDSKIIKQAEVTTKGSNEFVQEIEFESLTPGSVIVFRVSLDPKAQEAVGLLRHHLVQFSSHFKSGSLPDDKAVSILKTPFASIASKLSLADLNYVLYRCDAEEQEDGGGCYNVPNWTPLKYAGLQGLMSVMAEIRPKNDLGHPFCVNLRNGDWMIDYVSNRFIVHSGAPAEVGKWFQAMFTYLKQLPRYLIPCYFDAILTGVYTTLLDTSLKQMSEFIQKGSTFVKHLALGSLQMCGVGRFPALPPVSPSLQDVPYRINDIIKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFISLRGLMLVTGRHLEARNIILAFASTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYSKIVPNGIEILKCPVSRMYPSDSSPPLPAGTVEQPLYDVIQEAMQRHMQGIQYKERNAGPQIDRNMKDEGFNVTVGVDPVTGFVFGGNRFNCGTWMDKMGESERARNLGIPATPRDGSAVEIVGLSKSMVAWLMELHKKNVFPYKGVTVQKNEKSVIITYEEWNRKIQNNFEKLFYVSEDLIDLNEKHPNLIHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPDLFTPSKAWKALDMAEKKLLGPLGMKTLDPDDMVHCGVYDNALDSDNYNVAKGFNYHQGPEWLWPLGYFLRAKLYFSKLMGKETHAETIYLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATMLEVLYDL

Target ORF information:

RefSeq Version	XM_014490776.1
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014490776.1

ORF Insert Sequence:

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGTATATG 
CTGGACACCG CCAGGATGTT GAGGCCTAAC TTGTACTTAA TGGCTGAGCT GTTTACAGGA 
AGTGAAGAGC TGGACAATGT CTTTGTAACT AAACTTGGCA TTAGCTCCTT AATAAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AGL ( XM_006000010.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_006000072.1 Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X6, mRNA.

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CloneID	OLa30930
Clone ID Related Accession (Same CDS sequence)	XM_014490776.1 , XM_006000010.2
Accession Version	XM_006000010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4602bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	glycogen debranching enzyme isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819578.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_006000010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGTATATG 
CTGGACACCG CCAGGATGTT GAGGCCTAAC TTGTACTTAA TGGCTGAGCT GTTTACAGGA 
AGTGAAGAGC TGGACAATGT CTTTGTAACT AAACTTGGCA TTAGCTCCTT AATAAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006000072.1
CDS	215..4816
Translation	MMQNKQIRVLLLNDMEKLEKTVFKLEQGYELQFRLGPTLQGKAVTVTTNYPAPKEPFNRYKFRSLEWNNPTGREDDSDKYCKLDLQIAGSFQYFFGYGNDSKSGGGYIVVDPILCVGDNQVLPLDCITLVTYLAKCMGPFDEWEDRLKVAKESGYNMIHFTPLQTLGLSRSCYSLSDQLELNPDFSRSNEKYTWSDVGKLVEKLKKEWNVLCITDVVYNHTAANSKWIQEHPECGYNLVNSPHLKPAWIMDRALWHLTCDIADGKYIHKGLPALIENEQQLNNIRKVLWEDIYPKIKLWEFYQVDIDKAVQQFGTLLSQEKMMTKTKSDPKQHLKIIQDPEYKRFGCTVDMDVALESFIPHNNMPPAYVECCNWFRKRLEELNGERFREVNYHQEQAVNCILGNVLYERLAGHGPKLGPITRKHPLVTKYFAYRFQEMTLEQEELMMHQSNKACHFLAHNGWVMADDPLRNFAESDSNVYLRRELICWGDSVKLRYGNKPEDCPYLWAHMKKYTEITAKHFYGVRLDNCHSTPLHVAEYMLDTARMLRPNLYLMAELFTGSEELDNVFVTKLGISSLIREAMSAYNSHEEGRLVYRFGGEPVGSFVQPCLRPLVPTIAHALFMDVTHDNECPILHRSAYDSLSSSAIVSLASCATGSTRGYDEHVPHQISVVSEERYYSKWNPAADPSNPGEVNFQSGIIAGKRALNRLHQELGAKGFIQVYVDQVDEDIVAVTRHCPSTHQSVVAVSRTAFRDPKASYYRPDVPPMCIPGKIEEVVLEAKTVERNAIPYKKDEKSINGLPNLTVEIREHIQLKDSKIIKQAEVTTKGSNEFVQEIEFESLTPGSVIVFRVSLDPKAQEAVGLLRHHLVQFSSHFKSGSLPDDKAVSILKTPFASIASKLSLADLNYVLYRCDAEEQEDGGGCYNVPNWTPLKYAGLQGLMSVMAEIRPKNDLGHPFCVNLRNGDWMIDYVSNRFIVHSGAPAEVGKWFQAMFTYLKQLPRYLIPCYFDAILTGVYTTLLDTSLKQMSEFIQKGSTFVKHLALGSLQMCGVGRFPALPPVSPSLQDVPYRINDIIKEKEQCCVSLAAGLPHFSSGIFRCWGRDTFISLRGLMLVTGRHLEARNIILAFASTLRHGLIPNLLGQGTYARYNCRDAVWWWLQCIQDYSKIVPNGIEILKCPVSRMYPSDSSPPLPAGTVEQPLYDVIQEAMQRHMQGIQYKERNAGPQIDRNMKDEGFNVTVGVDPVTGFVFGGNRFNCGTWMDKMGESERARNLGIPATPRDGSAVEIVGLSKSMVAWLMELHKKNVFPYKGVTVQKNEKSVIITYEEWNRKIQNNFEKLFYVSEDLIDLNEKHPNLIHKRGIYKDSYGASSPWCDYQLRPNFTIAMVVAPDLFTPSKAWKALDMAEKKLLGPLGMKTLDPDDMVHCGVYDNALDSDNYNVAKGFNYHQGPEWLWPLGYFLRAKLYFSKLMGKETHAETIYLVKNVLSRHYVHLERSPWKGLPELTNENGQYCPFSCETQAWSIATMLEVLYDL

Target ORF information:

RefSeq Version	XM_006000010.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006000010.2

ORF Insert Sequence:

ATGATGCAAA ACAAACAAAT TAGAGTTTTG CTTCTAAATG ATATGGAGAA GCTTGAAAAG 
ACAGTTTTCA AGCTGGAACA AGGATATGAA CTCCAGTTTC GATTGGGTCC AACTTTACAA 
GGCAAAGCAG TGACTGTCAC CACTAATTAC CCTGCTCCCA AGGAGCCTTT TAATCGTTAC 
AAGTTCAGAT CATTAGAGTG GAACAACCCA ACAGGAAGAG AAGATGACTC TGATAAGTAC 
TGTAAACTTG ATCTTCAGAT AGCTGGGTCA TTCCAGTATT TTTTTGGTTA TGGAAATGAC 
AGCAAAAGTG GTGGAGGTTA TATAGTTGTA GACCCAATAC TTTGTGTTGG TGATAACCAG 
GTACTACCTT TGGACTGCAT CACTCTCGTG ACATATCTAG CAAAATGCAT GGGACCCTTT 
GATGAATGGG AGGATAGACT AAAAGTTGCC AAAGAATCAG GTTATAATAT GATCCACTTT 
ACTCCACTGC AGACACTTGG ATTGTCTAGA TCATGTTATT CCCTATCTGA CCAATTAGAA 
CTAAATCCAG ATTTCTCAAG ATCTAATGAA AAGTATACGT GGAGTGATGT TGGGAAGCTA 
GTGGAGAAAC TGAAGAAGGA ATGGAACGTG CTTTGTATTA CGGATGTTGT CTACAACCAT 
ACTGCTGCTA ATAGTAAGTG GATTCAGGAA CACCCAGAAT GTGGCTACAA CCTGGTAAAC 
TCTCCTCATC TGAAACCAGC CTGGATTATG GATAGAGCTT TGTGGCATTT GACCTGTGAT 
ATTGCTGATG GGAAATACAT ACATAAAGGG CTGCCTGCCC TTATTGAAAA TGAGCAACAG 
CTAAATAATA TTCGCAAAGT GCTGTGGGAA GATATATACC CAAAGATTAA ATTATGGGAG 
TTTTACCAAG TGGACATTGA CAAAGCTGTG CAGCAGTTTG GGACTCTCCT TTCTCAAGAG 
AAGATGATGA CTAAGACCAA ATCTGATCCC AAGCAGCATC TTAAGATTAT TCAAGACCCT 
GAATATAAAA GATTTGGTTG TACTGTGGAT ATGGACGTAG CACTGGAATC CTTTATACCA 
CATAACAATA TGCCACCTGC TTATGTGGAA TGTTGTAACT GGTTCCGAAA GAGACTTGAA 
GAGTTAAATG GCGAGAGATT CAGAGAAGTG AACTACCATC AGGAGCAGGC AGTCAACTGT 
ATTTTGGGAA ATGTTCTGTA TGAGCGTCTG GCAGGCCATG GTCCTAAGCT AGGTCCTATC 
ACTAGAAAAC ATCCATTAGT GACAAAATAT TTTGCATACA GATTCCAAGA AATGACCTTG 
GAGCAGGAAG AGTTAATGAT GCACCAGTCT AACAAAGCAT GTCACTTCCT GGCCCATAAT 
GGCTGGGTGA TGGCAGATGA TCCACTAAGA AATTTTGCTG AATCAGATTC AAATGTTTAC 
CTAAGGAGGG AGCTGATTTG CTGGGGTGAT AGTGTCAAAC TGCGCTATGG TAACAAACCA 
GAGGACTGTC CTTACCTCTG GGCGCACATG AAGAAGTACA CTGAAATCAC TGCAAAACAC 
TTCTATGGAG TACGACTGGA TAACTGTCAT TCAACTCCTC TTCATGTGGC TGAGTATATG 
CTGGACACCG CCAGGATGTT GAGGCCTAAC TTGTACTTAA TGGCTGAGCT GTTTACAGGA 
AGTGAAGAGC TGGACAATGT CTTTGTAACT AAACTTGGCA TTAGCTCCTT AATAAGAGAG 
GCGATGAGTG CTTACAACAG CCATGAAGAG GGAAGGCTAG TTTATCGTTT TGGAGGGGAG 
CCTGTTGGAT CATTTGTTCA GCCATGCTTG AGGCCTTTAG TTCCTACAAT TGCACATGCA 
CTGTTTATGG ATGTAACTCA TGACAATGAA TGCCCAATTC TGCATCGCTC TGCTTATGAT 
TCCCTCTCTA GTTCAGCAAT TGTGTCTTTG GCATCCTGTG CCACAGGAAG CACAAGAGGC 
TACGATGAAC ATGTACCTCA TCAGATCTCT GTTGTTTCTG AAGAAAGATA TTATAGCAAG 
TGGAATCCTG CAGCAGATCC ATCGAACCCC GGTGAGGTTA ATTTTCAGAG TGGGATTATA 
GCTGGAAAGC GGGCACTCAA TAGGCTGCAT CAAGAACTGG GAGCTAAAGG ATTTATCCAG 
GTGTATGTGG ATCAAGTTGA TGAAGACATA GTAGCAGTAA CCAGACACTG TCCAAGCACT 
CACCAGTCTG TAGTGGCTGT CTCTCGTACA GCTTTCCGGG ACCCTAAAGC CTCCTACTAT 
CGTCCAGATG TGCCTCCAAT GTGTATTCCA GGAAAAATTG AAGAGGTAGT GTTGGAGGCT 
AAGACTGTAG AGAGGAATGC TATTCCATAC AAAAAGGATG AGAAGAGTAT TAATGGGCTG 
CCAAACTTGA CAGTAGAAAT CAGAGAACAT ATTCAGCTTA AGGATAGCAA AATCATAAAA 
CAAGCTGAAG TAACAACAAA AGGAAGCAAT GAATTTGTGC AGGAAATAGA ATTTGAAAGT 
TTAACTCCTG GAAGCGTAAT TGTGTTCAGG GTTAGTCTCG ATCCAAAGGC TCAAGAAGCT 
GTAGGATTAC TTCGCCACCA CCTCGTCCAG TTCAGTTCTC ATTTTAAATC TGGCAGTTTG 
CCTGATGACA AAGCTGTATC CATTTTGAAG ACACCTTTTG CTTCTATTGC CTCTAAGTTA 
TCTTTGGCTG ACCTGAATTA TGTGCTGTAT CGCTGTGATG CAGAAGAACA AGAAGATGGT 
GGGGGATGCT ACAATGTACC AAACTGGACA CCTCTGAAAT ATGCAGGCCT GCAAGGGTTA 
ATGTCTGTTA TGGCTGAGAT CAGACCAAAA AATGACCTGG GGCATCCTTT CTGTGTTAAT 
TTGAGAAATG GAGATTGGAT GATTGATTAT GTCAGCAATC GTTTTATTGT CCATTCTGGA 
GCTCCTGCAG AGGTTGGTAA ATGGTTTCAA GCTATGTTTA CCTACTTGAA GCAGCTTCCT 
CGCTACCTTA TACCTTGTTA TTTTGATGCA ATCTTGACTG GAGTTTATAC AACTCTCTTG 
GATACTTCAT TGAAACAGAT GTCAGAATTT ATTCAGAAGG GGTCTACATT TGTGAAGCAT 
CTTGCCCTTG GTTCGCTCCA GATGTGTGGA GTAGGGAGGT TTCCTGCTCT TCCTCCTGTG 
TCTCCCTCCT TGCAGGATGT TCCTTATAGG ATTAATGATA TCATCAAAGA GAAGGAACAG 
TGTTGTGTAT CTTTAGCTGC TGGGTTGCCT CATTTTTCTT CTGGCATTTT CCGCTGCTGG 
GGAAGGGATA CGTTCATTTC ACTAAGAGGT TTGATGCTTG TTACTGGACG TCACTTGGAA 
GCCAGGAATA TAATTCTTGC CTTTGCCAGT ACCCTGAGAC ATGGACTTAT TCCAAACTTA 
CTTGGCCAGG GGACATATGC CAGATACAAT TGTCGTGATG CAGTATGGTG GTGGCTCCAG 
TGTATCCAGG ATTATAGTAA GATAGTACCA AATGGAATAG AAATCCTCAA GTGCCCTGTT 
TCAAGAATGT ATCCTTCAGA TTCTTCCCCA CCTCTGCCTG CAGGCACAGT GGAGCAGCCT 
CTCTATGATG TAATACAAGA AGCCATGCAG CGGCACATGC AGGGAATACA GTACAAGGAA 
AGGAATGCTG GACCTCAGAT AGACCGGAAT ATGAAGGATG AAGGTTTTAA TGTAACTGTA 
GGAGTGGACC CAGTAACTGG CTTTGTGTTT GGCGGAAACC GGTTTAACTG TGGCACATGG 
ATGGACAAAA TGGGAGAAAG TGAGAGGGCT CGCAACTTGG GTATTCCAGC AACACCAAGA 
GATGGGTCAG CTGTGGAAAT TGTGGGTCTG AGTAAATCCA TGGTTGCTTG GCTGATGGAG 
CTACATAAGA AAAATGTGTT TCCCTATAAA GGAGTCACAG TGCAAAAGAA TGAAAAATCT 
GTTATAATCA CCTATGAAGA GTGGAACAGA AAAATCCAGA ATAATTTTGA AAAGTTATTT 
TATGTTTCTG AGGACCTCAT TGATCTCAAC GAGAAGCACC CCAATTTGAT TCATAAGCGG 
GGAATTTACA AGGACAGCTA TGGAGCTTCA AGTCCATGGT GTGACTATCA GCTTAGACCA 
AACTTTACCA TAGCTATGGT TGTGGCTCCT GACTTGTTTA CACCAAGTAA AGCATGGAAG 
GCCTTAGACA TGGCAGAGAA AAAGCTCCTT GGGCCCCTGG GAATGAAAAC GTTGGACCCT 
GATGACATGG TACACTGTGG GGTTTATGAC AATGCTCTGG ACAGTGACAA CTACAACGTT 
GCTAAAGGTT TTAACTATCA CCAAGGACCT GAATGGCTGT GGCCACTCGG TTACTTCCTG 
CGGGCAAAAC TGTATTTCTC AAAGTTAATG GGAAAAGAAA CCCATGCGGA GACAATCTAC 
CTAGTAAAGA ATGTTCTTTC TCGTCATTAT GTTCATTTGG AGAGATCTCC TTGGAAGGGT 
CTTCCAGAGC TGACAAATGA AAATGGACAG TATTGCCCCT TCAGCTGCGA GACCCAGGCT 
TGGTCCATTG CTACTATGCT CGAAGTCCTT TACGACTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

AGL cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム