LOC104930911 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104930911
Entrez Gene ID	104930911
Full Name	regulator of nonsense transcripts 1
Synonyms	upf1
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019268609.1	XP_019124154.1	LOW QUALITY PROTEIN: regulator of nonsense transcripts 1
XM_027290310.1	XP_027146111.1	regulator of nonsense transcripts 1

Pathways from BioSystems
Gene Ontology

The following LOC104930911 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104930911 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa101211	XM_019268609.1 Latest version!	Larimichthys crocea regulator of nonsense transcripts 1 (LOC104930911), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLa208671	XM_027290310.1 Latest version!	Larimichthys crocea regulator of nonsense transcripts 1 (LOC104930911), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104930911 ( XM_019268609.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019124154.1 Larimichthys crocea regulator of nonsense transcripts 1 (LOC104930911), mRNA.

Price & Availability↑

CloneID	OLa101211
Clone ID Related Accession (Same CDS sequence)	XM_019268609.1
Accession Version	XM_019268609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	LOW QUALITY PROTEIN: regulator of nonsense transcripts 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608950.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 2 genomic stop codon ##RefSeq-Attributes-END##

ATGAGTGTGG AGGCGTACGG GCCGAGCTCT CAGACCCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGTTGC TCGGAGCGGA CACCCAGGGC TCCGAGTACG ACTTCACCGA TTTTACCCTT 
CCCAGCCAGA CGCAAACTCA AGGCCAGACC CAGAGCCAGC TGGACAACCA GGTGAACGGT 
CCCGATGAAG GTCTTCACAA CGGCGGCGTG GATGACTCGG TGGCCAAAGC CAGCCAGCTG 
TTGGCCGAGC TGAACTTTGA GGAGGATGAA GAGGACACAT ACTACACCAA AGACCTGCCT 
TCACACGCGT GCAGCTACTG TGGTATCCAC GATCCAGCGT GTGTGGTGTA CTGCAATACC 
AGCAAGAAGT GGTTCTGTAA TGGACGTGGC AACACATCTG GCAGTCACAT TGTTAACCAC 
CTGGTGAGAG CCAAATGCAA AGAGGTGACA CTGCATAAAG ATGGGCCACT GGGCGAGACG 
GTGCTGGAGT GTTACAACTG TGGCTGTCGC AACGTCTTCC TCCTGGGCTT CATCCCGGCC 
AAGGCCGATT CTGTGGTGGT GCTACTCTGC AGGCAGCCCT GTGCCAGTCA GAGTAGCCTG 
AAGGACATCA ACTGGGACAG CTCGCAGTGG CAACCACTGA TCCAGGACCG CTGCTTTCTG 
TCCTGGCTGG TGAAGATCCC ATCAGAGCAG GAGCAGCTTC GGGCCCGTCA GATCACCGCC 
CAGCAGATCA ACAAGTTGGA GGAGCTCTGG AAGGACAACC CCAGCGCCAC CTTGGAAGAC 
CTGGAGAAAC CGGGAGTGGA CGAGGAGCCC CAGCATGTGC TGCTGCGATA TGAGGACGCC 
TACCAGTACC AAAACATCTT TGGCCCTTTG GTCAAACTGG AGGCCGACTA CGACAAGAAG 
CTTAAGGAGT CCCAGACCCA AGACAATATA ACAGTCAGGT GGGACCTGGG GCTGAATAAA 
AAGCGGATTG CCTATTTCAC ACTGCCCAAG ACGGATTCAG ATATGCGGCT GATGCAAGGT 
GACGAGATCT GCTTGCGGTA CAAGGGAGAC CTGGCTCCGC TGTGGAAAGG AATCGGCCAT 
GTCATCAAAG TCCCTGACAA CTACGGGGAT GAAATTGCCA TTGAGTTGAG GAGCAGTGTG 
GGAGCACCTG TGGAAATCCC CCACAACTTC CAGGTGGACT TTGTGTGGAA GTCCACTTCC 
TTTGACAGGA TGCAGAGTGC CCTGAAGACA TTTGCGGTGG ATGAAACCTC CGTCTCTGGC 
TATATTTACC ACAAACTGCT GGGCCACGAA GTCGAGGATG TTACCATCAA GTGCCAGCTG 
CCAAAGCGCT TCACTGCCCA AGGCCTGCCC GACCTCAATC ACTCACAGGT GTATGCTGTG 
AAGACGGTGC TGCAGAGGCC TCTCAGTCTG ATCCAGGGTC CTCCTGGCAC TGGGAAAACT 
GTCACCTCTG CTACCATTGT CTATCACCTG TCTAGACAAG GCAATGGACC AGTCCTGGTG 
TGTGCTCCCA GTAACATTGC TGTGGACCAG TTGACTGAGA AGATTGACAA GACTGGACTG 
AAGGTCGTCA GGCTGTGTGC CAAGAGCCGC GAGGCCATCG AGTCACCAGT GTCCTTCCTG 
GCTCTGCATA ACCAGATCAG CAACATGGAC AGCATGCCAG AGCTTCAGAA ACTGCAGCAG 
CTGAAGGATG AGACTGGCGA GCTGTCATCT GCTGATGAGA AGCGCTACAG GGCTTTGAAA 
CGCACTGCTG AGAGGGAGCT GCTCATGAAT GCTGATGTTA TCTGTTGTAC CTGCGTTGGG 
GCTGGTGATC CTCGTCTGGC CAAGATGCAG TTCCGCTCCA TCCTGATTGA CGAGAGCACC 
CAGGCCACTG AGCCAGAGTG TATGGTCCCT GTGGTGCTGG GAGCTAAGCA GCTCATTCTG 
GTGGGTGATC ACTGCCAGTT GGGTCCTGTG GTGATGTGTA AGAAGGCAGC CAAAGCAGGT 
CTGTCCCAGT CCCTGTTTGA ACGCTTGGTG GTTCTGGGGA TCCGGCCAAT CCGCCTGCAG 
GTCCAGTACC GCATGCACCC GGCTCTCAGT GCCTTCCCCT CCAACATCTT CTATGAGGGC 
TCCCTGCAGA ATGGCGTCAC TGCAGCCGAC CGCATCAAGA AAGGCTTTGA CTTCCAGTGG 
CCTCAGCCAG ACAAGCCCAT GTTTTTCTAC GTGACTCAGG GTCAGGAGGA GATTGCCAGC 
TCTGGAACAT CGTACCTTAA CAGGACTGAG GCTGCCAACG TAGAGAAAAT TACCACAAGG 
CTGCTGAAGG CTGGAGCCAA ACCTGACCAG ATTGGCATCA TCACCCCGTA CGAGGGACAG 
CGCTCCTATT TGGTGCAGTA CATGCAGTTC AGCGGCTCCC TGCACACCAA ACTCTATCAG 
CAAGTGGAAA TTGCCAGCGT GGACGCCTTC CAAGGCAGAG AGAAGGATTT CATCATCCTG 
TCTTGTGTTC GTGCCAATGA GCACCAGGGC ATCGGCTTTC TGAATGACCC ACGCCGTCTC 
AACGTGGCGT TGACCCGAGC CAAGTAAAAA CCTCAGAACT GTTCAGACAT CATAGTGGAA 
GCAAATCGAA TACAGCTAAC AACTAGACAG CTTTTTAATG TATTATGGTG ACTTCAGAAA 
GTCCTTGTGG AGGGACCTCT CAATAACCTG AGGGAGAGTC TCATGCAGTT CAGCAAGCCC 
CGCAAACTGG TCAACACCAT CAACCCTGGG GGACGTTTTA TGAGCACTGC GATGTACGAT 
GCCCGTGAAG CCCTCATCCC TGGCTCTGCC TACGATCGCA GCAACGCTGC TGGACGTCCG 
TCCAACATGT ACTTTCAAAC TCACGACCAG ATTGGGATGA TTGGAGCGGG GCCTGGTCAC 
ATGGCTGCCA TGAATATTCC CATACCCTTC AACCTGGTGA TGCCACCAAT GCCTCCCCCC 
AGTTACCTGG GTCAGACCAA TGGCCCTGCT GCAGGGCGTG GAGCTATGAA GGGTAAGCCT 
GGGCGTGGCG GGCGACAGAG GGTCCGTGGA GGAAACCAGG GTGCCAGTCA GGGTAATGGA 
CCAAACAGTC AGGCCAGCCA GGATGGGGCC TCCCAGTCCT TCTCCCAAGG GCCACTGACA 
CAGGGCTACA TCTCCATGAG CCAGCCTTCT CAGATGAGCC AACCTGGCCT CTCCCAACCG 
GAACTGTCCC AGGACAGCTA CCTGGGCGAC GAGTTCAAGT CCCAAATCGA TGTGGCTTTG 
TCCCAGGACT CGACTTACCA GGGCGAACGT GCATACCAGC ATGGTGGGGT AACTGGACTG 
TCACAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019124154.1
CDS	222..3533
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEYDFTDFTLPSQTQTQGQTQSQLDNQVNGPDEGLHNGGVDDSVAKASQLLAELNFEEDEEDTYYTKDLPSHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKDNPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEIPHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVTIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLSRQGNGPVLVCAPSNIAVDQLTEKIDKTGLKVVRLCAKSREAIESPVSFLALHNQISNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRIKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQQVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRAKKPQNCSDIIVEANRIQLTTRQLFNVLWLQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGGRFMSTAMYDAREALIPGSAYDRSNAAGRPSNMYFQTHDQIGMIGAGPGHMAAMNIPIPFNLVMPPMPPPSYLGQTNGPAAGRGAMKGKPGRGGRQRVRGGNQGASQGNGPNSQASQDGASQSFSQGPLTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_019268609.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea regulator of nonsense transcripts 1 (LOC104930911), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019268609.1

ORF Insert Sequence:

ATGAGTGTGG AGGCGTACGG GCCGAGCTCT CAGACCCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGTTGC TCGGAGCGGA CACCCAGGGC TCCGAGTACG ACTTCACCGA TTTTACCCTT 
CCCAGCCAGA CGCAAACTCA AGGCCAGACC CAGAGCCAGC TGGACAACCA GGTGAACGGT 
CCCGATGAAG GTCTTCACAA CGGCGGCGTG GATGACTCGG TGGCCAAAGC CAGCCAGCTG 
TTGGCCGAGC TGAACTTTGA GGAGGATGAA GAGGACACAT ACTACACCAA AGACCTGCCT 
TCACACGCGT GCAGCTACTG TGGTATCCAC GATCCAGCGT GTGTGGTGTA CTGCAATACC 
AGCAAGAAGT GGTTCTGTAA TGGACGTGGC AACACATCTG GCAGTCACAT TGTTAACCAC 
CTGGTGAGAG CCAAATGCAA AGAGGTGACA CTGCATAAAG ATGGGCCACT GGGCGAGACG 
GTGCTGGAGT GTTACAACTG TGGCTGTCGC AACGTCTTCC TCCTGGGCTT CATCCCGGCC 
AAGGCCGATT CTGTGGTGGT GCTACTCTGC AGGCAGCCCT GTGCCAGTCA GAGTAGCCTG 
AAGGACATCA ACTGGGACAG CTCGCAGTGG CAACCACTGA TCCAGGACCG CTGCTTTCTG 
TCCTGGCTGG TGAAGATCCC ATCAGAGCAG GAGCAGCTTC GGGCCCGTCA GATCACCGCC 
CAGCAGATCA ACAAGTTGGA GGAGCTCTGG AAGGACAACC CCAGCGCCAC CTTGGAAGAC 
CTGGAGAAAC CGGGAGTGGA CGAGGAGCCC CAGCATGTGC TGCTGCGATA TGAGGACGCC 
TACCAGTACC AAAACATCTT TGGCCCTTTG GTCAAACTGG AGGCCGACTA CGACAAGAAG 
CTTAAGGAGT CCCAGACCCA AGACAATATA ACAGTCAGGT GGGACCTGGG GCTGAATAAA 
AAGCGGATTG CCTATTTCAC ACTGCCCAAG ACGGATTCAG ATATGCGGCT GATGCAAGGT 
GACGAGATCT GCTTGCGGTA CAAGGGAGAC CTGGCTCCGC TGTGGAAAGG AATCGGCCAT 
GTCATCAAAG TCCCTGACAA CTACGGGGAT GAAATTGCCA TTGAGTTGAG GAGCAGTGTG 
GGAGCACCTG TGGAAATCCC CCACAACTTC CAGGTGGACT TTGTGTGGAA GTCCACTTCC 
TTTGACAGGA TGCAGAGTGC CCTGAAGACA TTTGCGGTGG ATGAAACCTC CGTCTCTGGC 
TATATTTACC ACAAACTGCT GGGCCACGAA GTCGAGGATG TTACCATCAA GTGCCAGCTG 
CCAAAGCGCT TCACTGCCCA AGGCCTGCCC GACCTCAATC ACTCACAGGT GTATGCTGTG 
AAGACGGTGC TGCAGAGGCC TCTCAGTCTG ATCCAGGGTC CTCCTGGCAC TGGGAAAACT 
GTCACCTCTG CTACCATTGT CTATCACCTG TCTAGACAAG GCAATGGACC AGTCCTGGTG 
TGTGCTCCCA GTAACATTGC TGTGGACCAG TTGACTGAGA AGATTGACAA GACTGGACTG 
AAGGTCGTCA GGCTGTGTGC CAAGAGCCGC GAGGCCATCG AGTCACCAGT GTCCTTCCTG 
GCTCTGCATA ACCAGATCAG CAACATGGAC AGCATGCCAG AGCTTCAGAA ACTGCAGCAG 
CTGAAGGATG AGACTGGCGA GCTGTCATCT GCTGATGAGA AGCGCTACAG GGCTTTGAAA 
CGCACTGCTG AGAGGGAGCT GCTCATGAAT GCTGATGTTA TCTGTTGTAC CTGCGTTGGG 
GCTGGTGATC CTCGTCTGGC CAAGATGCAG TTCCGCTCCA TCCTGATTGA CGAGAGCACC 
CAGGCCACTG AGCCAGAGTG TATGGTCCCT GTGGTGCTGG GAGCTAAGCA GCTCATTCTG 
GTGGGTGATC ACTGCCAGTT GGGTCCTGTG GTGATGTGTA AGAAGGCAGC CAAAGCAGGT 
CTGTCCCAGT CCCTGTTTGA ACGCTTGGTG GTTCTGGGGA TCCGGCCAAT CCGCCTGCAG 
GTCCAGTACC GCATGCACCC GGCTCTCAGT GCCTTCCCCT CCAACATCTT CTATGAGGGC 
TCCCTGCAGA ATGGCGTCAC TGCAGCCGAC CGCATCAAGA AAGGCTTTGA CTTCCAGTGG 
CCTCAGCCAG ACAAGCCCAT GTTTTTCTAC GTGACTCAGG GTCAGGAGGA GATTGCCAGC 
TCTGGAACAT CGTACCTTAA CAGGACTGAG GCTGCCAACG TAGAGAAAAT TACCACAAGG 
CTGCTGAAGG CTGGAGCCAA ACCTGACCAG ATTGGCATCA TCACCCCGTA CGAGGGACAG 
CGCTCCTATT TGGTGCAGTA CATGCAGTTC AGCGGCTCCC TGCACACCAA ACTCTATCAG 
CAAGTGGAAA TTGCCAGCGT GGACGCCTTC CAAGGCAGAG AGAAGGATTT CATCATCCTG 
TCTTGTGTTC GTGCCAATGA GCACCAGGGC ATCGGCTTTC TGAATGACCC ACGCCGTCTC 
AACGTGGCGT TGACCCGAGC CAAGTAAAAA CCTCAGAACT GTTCAGACAT CATAGTGGAA 
GCAAATCGAA TACAGCTAAC AACTAGACAG CTTTTTAATG TATTATGGTG ACTTCAGAAA 
GTCCTTGTGG AGGGACCTCT CAATAACCTG AGGGAGAGTC TCATGCAGTT CAGCAAGCCC 
CGCAAACTGG TCAACACCAT CAACCCTGGG GGACGTTTTA TGAGCACTGC GATGTACGAT 
GCCCGTGAAG CCCTCATCCC TGGCTCTGCC TACGATCGCA GCAACGCTGC TGGACGTCCG 
TCCAACATGT ACTTTCAAAC TCACGACCAG ATTGGGATGA TTGGAGCGGG GCCTGGTCAC 
ATGGCTGCCA TGAATATTCC CATACCCTTC AACCTGGTGA TGCCACCAAT GCCTCCCCCC 
AGTTACCTGG GTCAGACCAA TGGCCCTGCT GCAGGGCGTG GAGCTATGAA GGGTAAGCCT 
GGGCGTGGCG GGCGACAGAG GGTCCGTGGA GGAAACCAGG GTGCCAGTCA GGGTAATGGA 
CCAAACAGTC AGGCCAGCCA GGATGGGGCC TCCCAGTCCT TCTCCCAAGG GCCACTGACA 
CAGGGCTACA TCTCCATGAG CCAGCCTTCT CAGATGAGCC AACCTGGCCT CTCCCAACCG 
GAACTGTCCC AGGACAGCTA CCTGGGCGAC GAGTTCAAGT CCCAAATCGA TGTGGCTTTG 
TCCCAGGACT CGACTTACCA GGGCGAACGT GCATACCAGC ATGGTGGGGT AACTGGACTG 
TCACAGTACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104930911 ( XM_027290310.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027146111.1 Larimichthys crocea regulator of nonsense transcripts 1 (LOC104930911), mRNA.

Price & Availability↑

CloneID	OLa208671
Clone ID Related Accession (Same CDS sequence)	XM_027290310.1
Accession Version	XM_027290310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	regulator of nonsense transcripts 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040027.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG AGGCGTACGG GCCGAGCTCT CAGACCCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGTTGC TCGGAGCGGA CACCCAGGGC TCCGAGTACG ACTTCACCGA TTTTACCCTT 
CCCAGCCAGA CGCAAACTCA AGGCCAGACC CAGAGCCAGC TGGACAACCA GGTGAACGGT 
CCCGATGAAG GTCTTCACAA CGGCGGCGTG GAGGACTCGG TGGCCAAAGC CAGCCAGCTG 
TTGGCCGAGC TGAACTTTGA GGAGGATGAA GAGGACACAT ACTACACCAA AGACCTGCCT 
TCACACGCGT GCAGCTACTG TGGTATCCAC GATCCAGCGT GTGTGGTGTA CTGCAATACC 
AGCAAGAAGT GGTTCTGTAA TGGACGTGGC AACACATCTG GCAGTCACAT TGTTAACCAC 
CTGGTGAGAG CCAAATGCAA AGAGGTGACA CTGCATAAAG ATGGGCCACT GGGCGAGACG 
GTGCTGGAGT GTTACAACTG TGGCTGTCGC AACGTCTTCC TCCTGGGCTT CATCCCGGCC 
AAGGCCGATT CTGTGGTGGT GCTACTCTGC AGGCAGCCCT GTGCCAGTCA GAGTAGCCTG 
AAGGACATCA ACTGGGACAG CTCGCAGTGG CAACCACTGA TCCAGGACCG CTGCTTTCTG 
TCCTGGCTGG TGAAGATCCC ATCAGAGCAG GAGCAGCTTC GGGCCCGTCA GATCACCGCC 
CAGCAGATCA ACAAGTTGGA GGAGCTCTGG AAGGACAACC CCAGCGCCAC CTTGGAAGAC 
CTGGAGAAAC CGGGAGTGGA CGAGGAGCCC CAGCATGTGC TGCTGCGATA TGAGGACGCC 
TACCAGTACC AAAACATCTT TGGCCCTTTG GTCAAACTGG AGGCCGACTA CGACAAGAAG 
CTTAAGGAGT CCCAGACCCA AGACAATATA ACAGTCAGGT GGGACCTGGG GCTGAATAAA 
AAGCGGATTG CCTATTTCAC ACTGCCCAAG ACGGATTCAG GTGATATGCG GCTGATGCAA 
GGTGACGAGA TCTGCTTGCG GTACAAGGGA GACCTGGCTC CGCTGTGGAA AGGAATCGGC 
CATGTCATCA AAGTCCCTGA CAACTACGGG GATGAAATTG CCATTGAGTT GAGGAGCAGT 
GTGGGAGCAC CTGTGGAAAT CCCCCACAAC TTCCAGGTGG ACTTTGTGTG GAAGTCCACT 
TCCTTTGACA GGATGCAGAG TGCCCTGAAG ACATTTGCGG TGGATGAAAC CTCCGTCTCT 
GGCTATATTT ACCACAAACT GCTGGGCCAC GAAGTCGAGG ATGTTACCAT CAAGTGCCAG 
CTGCCAAAGC GCTTCACTGC CCAAGGCCTG CCCGACCTCA ATCACTCACA GGTGTATGCT 
GTGAAGACGG TGCTGCAGAG GCCTCTCAGT CTGATCCAGG GTCCTCCTGG CACTGGGAAA 
ACTGTCACTT CTGCTACCAT TGTCTATCAC CTGTCTAGAC AAGGCAATGG ACCAGTCCTG 
GTGTGTGCTC CCAGTAACAT TGCTGTGGAC CAGTTGACTG AGAAGATTGA CAAGACTGGA 
CTGAAGGTCG TCAGGCTGTG TGCCAAGAGC CGCGAGGCCA TCGAGTCACC AGTGTCCTTC 
CTGGCTCTGC ATAACCAGAT CAGCAACATG GACAGCATGC CAGAGCTTCA GAAACTGCAG 
CAGCTGAAGG ATGAGACTGG CGAGCTGTCA TCTGCTGATG AGAAGCGCTA CAGGGCTTTG 
AAACGCACTG CTGAGAGGGA GCTGCTCATG AATGCTGATG TTATCTGTTG TACCTGCGTT 
GGGGCTGGTG ATCCTCGTCT GGCCAAGATG CAGTTCCGCT CCATCCTGAT TGACGAGAGC 
ACCCAGGCCA CTGAGCCAGA GTGTATGGTC CCTGTGGTGC TGGGAGCTAA GCAGCTCATT 
CTGGTGGGTG ATCACTGCCA GTTGGGTCCT GTGGTGATGT GTAAGAAGGC AGCCAAAGCA 
GGTCTGTCCC AGTCCCTGTT TGAACGCTTG GTGGTTCTGG GGATCCGGCC AATCCGCCTG 
CAGGTCCAGT ACCGCATGCA CCCGGCTCTC AGTGCCTTCC CCTCCAACAT CTTCTATGAG 
GGCTCCCTGC AGAATGGCGT CACTGCAGCC GACCGCATCA AGAAAGGCTT TGACTTCCAG 
TGGCCTCAGC CAGACAAGCC CATGTTTTTC TACGTGACTC AGGGTCAGGA GGAGATTGCC 
AGCTCTGGAA CATCGTACCT TAACAGGACT GAGGCTGCCA ACGTAGAGAA AATTACCACA 
AGGCTGCTGA AGGCTGGAGC CAAACCTGAC CAGATTGGCA TCATCACCCC GTATGAGGGA 
CAGCGCTCCT ATTTGGTGCA GTACATGCAG TTCAGCGGCT CCCTGCACAC CAAACTCTAT 
CAGCAAGTGG AAATTGCCAG CGTGGACGCC TTCCAAGGCA GAGAGAAGGA TTTCATCATC 
CTGTCTTGTG TTCGTGCCAA TGAGCACCAG GGCATCGGCT TTCTGAATGA CCCACGCCGT 
CTCAACGTGG CGTTGACCCG AGCCAAGTAT GGTGTGATCA TCGTGGGGAA CCCCAAGGCG 
CTCTCCAAGC AGCCGCTCTG GAACAACCTG CTGAACAACT ACAAGGAGCA GAAAGTCCTT 
GTGGAGGGAC CTCTCAATAA CCTGAGGGAG AGTCTCATGC AGTTCAGCAA GCCCCGCAAA 
CTGGTCAACA CCATCAACCC TGGGGGACGT TTTATGAGCA CTGCGATGTA CGATGCCCGT 
GAAGCCCTCA TCCCTGGCTC TGCCTACGAT CGCAGCAACG CTGCTGGACG TCCGTCCAAC 
ATGTACTTTC AAACTCACGA CCAGATTGGG ATGATTGGAG CGGGGCCTGG TCACATGGCC 
GCCATGAATA TTCCCATACC CTTCAACCTG GTGATGCCAC CAATGCCCCC CCCCAGTTAC 
CTGGGTCAGA CCAACGGCCC TGCTGCAGGG CGTGGAGCTA TGAAGGGTAA GCCTGGGCGT 
GGCGGGCGAC AGAGGGTCCG TGGAGGAAAC CAGGGTGCCA GTCAGGGTAA TGGACCAAAC 
AGTCAGGCCA GCCAGGATGG GGCCTCCCAG TCCTTCTCCC AGGGGCCACT GACACAGGGC 
TACATCTCCA TGAGCCAGCC TTCTCAGATG AGCCAACCTG GCCTCTCCCA ACCGGAACTG 
TCCCAGGACA GCTACCTGGG CGACGAGTTC AAGTCCCAAA TCGATGTGGC TTTGTCCCAG 
GACTCGACTT ACCAGGGCGA ACGTGCATAC CAGCATGGTG GGGTAACTGG ACTGTCACAG 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027146111.1
CDS	277..3582
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEYDFTDFTLPSQTQTQGQTQSQLDNQVNGPDEGLHNGGVEDSVAKASQLLAELNFEEDEEDTYYTKDLPSHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKDNPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEIPHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVTIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLSRQGNGPVLVCAPSNIAVDQLTEKIDKTGLKVVRLCAKSREAIESPVSFLALHNQISNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRIKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQQVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRAKYGVIIVGNPKALSKQPLWNNLLNNYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGGRFMSTAMYDAREALIPGSAYDRSNAAGRPSNMYFQTHDQIGMIGAGPGHMAAMNIPIPFNLVMPPMPPPSYLGQTNGPAAGRGAMKGKPGRGGRQRVRGGNQGASQGNGPNSQASQDGASQSFSQGPLTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_027290310.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea regulator of nonsense transcripts 1 (LOC104930911), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027290310.1

ORF Insert Sequence:

ATGAGTGTGG AGGCGTACGG GCCGAGCTCT CAGACCCTCA CCTTCCTGGA CACCGAGGAA 
GCGGAGTTGC TCGGAGCGGA CACCCAGGGC TCCGAGTACG ACTTCACCGA TTTTACCCTT 
CCCAGCCAGA CGCAAACTCA AGGCCAGACC CAGAGCCAGC TGGACAACCA GGTGAACGGT 
CCCGATGAAG GTCTTCACAA CGGCGGCGTG GAGGACTCGG TGGCCAAAGC CAGCCAGCTG 
TTGGCCGAGC TGAACTTTGA GGAGGATGAA GAGGACACAT ACTACACCAA AGACCTGCCT 
TCACACGCGT GCAGCTACTG TGGTATCCAC GATCCAGCGT GTGTGGTGTA CTGCAATACC 
AGCAAGAAGT GGTTCTGTAA TGGACGTGGC AACACATCTG GCAGTCACAT TGTTAACCAC 
CTGGTGAGAG CCAAATGCAA AGAGGTGACA CTGCATAAAG ATGGGCCACT GGGCGAGACG 
GTGCTGGAGT GTTACAACTG TGGCTGTCGC AACGTCTTCC TCCTGGGCTT CATCCCGGCC 
AAGGCCGATT CTGTGGTGGT GCTACTCTGC AGGCAGCCCT GTGCCAGTCA GAGTAGCCTG 
AAGGACATCA ACTGGGACAG CTCGCAGTGG CAACCACTGA TCCAGGACCG CTGCTTTCTG 
TCCTGGCTGG TGAAGATCCC ATCAGAGCAG GAGCAGCTTC GGGCCCGTCA GATCACCGCC 
CAGCAGATCA ACAAGTTGGA GGAGCTCTGG AAGGACAACC CCAGCGCCAC CTTGGAAGAC 
CTGGAGAAAC CGGGAGTGGA CGAGGAGCCC CAGCATGTGC TGCTGCGATA TGAGGACGCC 
TACCAGTACC AAAACATCTT TGGCCCTTTG GTCAAACTGG AGGCCGACTA CGACAAGAAG 
CTTAAGGAGT CCCAGACCCA AGACAATATA ACAGTCAGGT GGGACCTGGG GCTGAATAAA 
AAGCGGATTG CCTATTTCAC ACTGCCCAAG ACGGATTCAG GTGATATGCG GCTGATGCAA 
GGTGACGAGA TCTGCTTGCG GTACAAGGGA GACCTGGCTC CGCTGTGGAA AGGAATCGGC 
CATGTCATCA AAGTCCCTGA CAACTACGGG GATGAAATTG CCATTGAGTT GAGGAGCAGT 
GTGGGAGCAC CTGTGGAAAT CCCCCACAAC TTCCAGGTGG ACTTTGTGTG GAAGTCCACT 
TCCTTTGACA GGATGCAGAG TGCCCTGAAG ACATTTGCGG TGGATGAAAC CTCCGTCTCT 
GGCTATATTT ACCACAAACT GCTGGGCCAC GAAGTCGAGG ATGTTACCAT CAAGTGCCAG 
CTGCCAAAGC GCTTCACTGC CCAAGGCCTG CCCGACCTCA ATCACTCACA GGTGTATGCT 
GTGAAGACGG TGCTGCAGAG GCCTCTCAGT CTGATCCAGG GTCCTCCTGG CACTGGGAAA 
ACTGTCACTT CTGCTACCAT TGTCTATCAC CTGTCTAGAC AAGGCAATGG ACCAGTCCTG 
GTGTGTGCTC CCAGTAACAT TGCTGTGGAC CAGTTGACTG AGAAGATTGA CAAGACTGGA 
CTGAAGGTCG TCAGGCTGTG TGCCAAGAGC CGCGAGGCCA TCGAGTCACC AGTGTCCTTC 
CTGGCTCTGC ATAACCAGAT CAGCAACATG GACAGCATGC CAGAGCTTCA GAAACTGCAG 
CAGCTGAAGG ATGAGACTGG CGAGCTGTCA TCTGCTGATG AGAAGCGCTA CAGGGCTTTG 
AAACGCACTG CTGAGAGGGA GCTGCTCATG AATGCTGATG TTATCTGTTG TACCTGCGTT 
GGGGCTGGTG ATCCTCGTCT GGCCAAGATG CAGTTCCGCT CCATCCTGAT TGACGAGAGC 
ACCCAGGCCA CTGAGCCAGA GTGTATGGTC CCTGTGGTGC TGGGAGCTAA GCAGCTCATT 
CTGGTGGGTG ATCACTGCCA GTTGGGTCCT GTGGTGATGT GTAAGAAGGC AGCCAAAGCA 
GGTCTGTCCC AGTCCCTGTT TGAACGCTTG GTGGTTCTGG GGATCCGGCC AATCCGCCTG 
CAGGTCCAGT ACCGCATGCA CCCGGCTCTC AGTGCCTTCC CCTCCAACAT CTTCTATGAG 
GGCTCCCTGC AGAATGGCGT CACTGCAGCC GACCGCATCA AGAAAGGCTT TGACTTCCAG 
TGGCCTCAGC CAGACAAGCC CATGTTTTTC TACGTGACTC AGGGTCAGGA GGAGATTGCC 
AGCTCTGGAA CATCGTACCT TAACAGGACT GAGGCTGCCA ACGTAGAGAA AATTACCACA 
AGGCTGCTGA AGGCTGGAGC CAAACCTGAC CAGATTGGCA TCATCACCCC GTATGAGGGA 
CAGCGCTCCT ATTTGGTGCA GTACATGCAG TTCAGCGGCT CCCTGCACAC CAAACTCTAT 
CAGCAAGTGG AAATTGCCAG CGTGGACGCC TTCCAAGGCA GAGAGAAGGA TTTCATCATC 
CTGTCTTGTG TTCGTGCCAA TGAGCACCAG GGCATCGGCT TTCTGAATGA CCCACGCCGT 
CTCAACGTGG CGTTGACCCG AGCCAAGTAT GGTGTGATCA TCGTGGGGAA CCCCAAGGCG 
CTCTCCAAGC AGCCGCTCTG GAACAACCTG CTGAACAACT ACAAGGAGCA GAAAGTCCTT 
GTGGAGGGAC CTCTCAATAA CCTGAGGGAG AGTCTCATGC AGTTCAGCAA GCCCCGCAAA 
CTGGTCAACA CCATCAACCC TGGGGGACGT TTTATGAGCA CTGCGATGTA CGATGCCCGT 
GAAGCCCTCA TCCCTGGCTC TGCCTACGAT CGCAGCAACG CTGCTGGACG TCCGTCCAAC 
ATGTACTTTC AAACTCACGA CCAGATTGGG ATGATTGGAG CGGGGCCTGG TCACATGGCC 
GCCATGAATA TTCCCATACC CTTCAACCTG GTGATGCCAC CAATGCCCCC CCCCAGTTAC 
CTGGGTCAGA CCAACGGCCC TGCTGCAGGG CGTGGAGCTA TGAAGGGTAA GCCTGGGCGT 
GGCGGGCGAC AGAGGGTCCG TGGAGGAAAC CAGGGTGCCA GTCAGGGTAA TGGACCAAAC 
AGTCAGGCCA GCCAGGATGG GGCCTCCCAG TCCTTCTCCC AGGGGCCACT GACACAGGGC 
TACATCTCCA TGAGCCAGCC TTCTCAGATG AGCCAACCTG GCCTCTCCCA ACCGGAACTG 
TCCCAGGACA GCTACCTGGG CGACGAGTTC AAGTCCCAAA TCGATGTGGC TTTGTCCCAG 
GACTCGACTT ACCAGGGCGA ACGTGCATAC CAGCATGGTG GGGTAACTGG ACTGTCACAG 
TACTAG