LOC104920975 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104920975
Entrez Gene ID	104920975
Full Name	regulator of nonsense transcripts 1
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019260030.1	XP_019115575.1	regulator of nonsense transcripts 1
XM_019260030.2	XP_019115575.1	regulator of nonsense transcripts 1

Pathways from BioSystems
Gene Ontology

The following LOC104920975 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104920975 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa201944	XM_019260030.2 Latest version!	Larimichthys crocea regulator of nonsense transcripts 1 (LOC104920975), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OLa90792	XM_019260030.1 Latest version!	Larimichthys crocea regulator of nonsense transcripts 1 (LOC104920975), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104920975 ( XM_019260030.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019115575.1 Larimichthys crocea regulator of nonsense transcripts 1 (LOC104920975), mRNA.

Price & Availability↑

CloneID	OLa201944
Clone ID Related Accession (Same CDS sequence)	XM_019260030.2
Accession Version	XM_019260030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	regulator of nonsense transcripts 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040022.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019260030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTCG AGGCGTACGG GCCGAGCTCG CAGACTCTCA CGTTCCTCGA CACCGAGGAG 
ACCGAGCTGC TGGGAGCGGA CACCCAGGGC TCCGAGTACG ACTTCACCGA CTTCACCCTG 
CCGAGCCAGA CCCAGACCCA AGGCCAGACC CAGAGCCAGC TGGACGGACA GGTTAATGGT 
CCTGATGGTG TGCTGCAGAA TGGAGACGAC CCTGTGGTGA AAGCCAGTCA GCTGCTGGCA 
GAGTTGAACT TTGAGGAAGA TGAGGAGGAC ACCTACTACA CCAAGGACCT ACCTTCACAC 
GCCTGCAGTT ACTGTGGGAT TCATGATCCA GCCTGCGTGG TCTACTGCAA CACCAGCAAG 
AAGTGGTTCT GTAATGGCCG TGGAAACACG TCCGGAAGCC ACATTGTAAA CCACCTGGTG 
AGAGCAAAGT CCAAAGAGGT GACCCTACAT AAAGATGGCC CTCTGGGGGA AACTGTATTG 
GAGTGCTACA ACTGTGGCTG TCGCAATGTC TTCCTTCTGG GCTTCATCCC TGCCAAAGCT 
GACTCAGTAG TGGTGTTACT GTGCAGGCAG CCATGTGCGA GCCAGAGCAG CCTGAAGGAC 
ATCAACTGGG ACAGCTCACA GTGGCAGCCT CTCATCCAGG ACCGCTGCTT TCTGTCCTGG 
CTCGTCAAAA TCCCTTCAGA GCAGGAGCAG CTGAGGGCTC GTCAAATCAC CGCCCAGCAG 
ATCAACAAGC TGGAGGAACT CTGGAAGGAA AATCCCACTG CCACTTTGGA GGATCTGGAG 
AAGCCTGGTG TGGACGAGGA GCCTCAACAT GTGCTGCTCC GCTATGAGGA TGCCTACCAG 
TACCAGAACA TCTTTGGTCC TCTTGTGAAG CTGGAGGCTG ACTATGATAA GAAACTCAAA 
GAGTCTCAGA CCCAAGACAA CATCACAGTC AGATGGGACT TGGGCCTGAA TAAAAAGAGG 
ATTGCTTATT TCACTCTTCC CAAGACAGAC TCTGACATGC GACTGATGCA GGGAGATGAG 
ATTTGCCTGA GGTACAAAGG AGACCTGGCC CCTCCGTGGA AAGGCATTGG ACATGTCATC 
AAAGTCCCTG ATAACTATGG TGATGAGATT GCCATAGAGC TGAGGAGCAG TGCCGGCGCT 
CCAGTGGAGA TCCCACACAA CTTTCAGGTT GACTTTGTGT GGAAGTCCAC TTCCTTTGAC 
AGGATGCAAA GTGCGCTAAA GACATTTGCT GTGGATGAGA CTTCAGTGTC GGGCTACATC 
TACCACAAAC TGCTCGGACA TGAGGTTGAG GATGTGGTCA TCAAGTGCCA GCTGCCCAAA 
CGCTTCACTG CTCAAGGCCT GCCTGACCTT AACCACTCAC AGGTGTATGC TGTGAAGACA 
GTCCTGCAGC GTCCCCTCAG TCTGATCCAG GGTCCTCCTG GCACCGGGAA GACTGTAACC 
TCTGCGACCA TCGTCTACCA CCTGGCTAGA CAGGGCAACG GGCCTGTGCT GGTGTGTGCT 
CCCAGTAACA TCGCTGTCGA CCAGCTGACA GAAAAGATCC ACCAGACAGG CCTCAAGGTG 
GTGAGGCTGT GTGCCAAGAG CAGAGAGGCC ATCGACTCAC CGGTTTCCTT CCTGGCTCTG 
CACAACCAGA CCCGTAACAT GGACAGTATG CCCGAACTTC AGAAGCTCCA GCAGCTGAAG 
GACGAGACTG GAGAGCTGTC CTCTTCGGAC GAGAAGCGCT ACAGAGCTCT GAGACGCACT 
GCTGAGAGGG AGCTGCTCAT GAATGCCGAT GTGATCTGCT GTACCTGTGT TGGGGCGGGT 
GATCCACGTC TGGCCAAGAT GCAGTTCCGC TCAATCCTGA TCGATGAAAG CACCCAGGCC 
ACCGAACCGG AGTGCATGGT GCCTGTTGTC CTCGGGGCTA AACAGTTGAT TCTGGTGGGT 
GATCACTGCC AGCTGGGCCC CGTGGTGATG TGTAAGAAGG CAGCTAAAGC AGGTCTGTCT 
CAGTCTCTGT TTGAGCGTCT GGTAGTTTTG GGGATCCGGC CAATCCGTCT GCAGGTCCAG 
TACCGCATGC ACCCGGCACT CAGCGCCTTC CCTTCCAACA TCTTCTACGA GGGCTCTTTG 
CAGAACGGAG TCACTGCCGC GGACCGTGTG AAAAAAGGCT TCGACTTCCA GTGGCCACAG 
CCTGACAAGC CGATGTTCTT TTATGTTACC CAAGGCCAAG AGGAAATTGC CAGCTCTGGA 
ACATCCTATC TCAACAGGAC GGAGGCTGCC AATGTGGAGA AAATAACCAC AAGGTTGCTG 
AAGGCCGGAG CAAAACCTGA TCAGATCGGT ATCATCACAC CCTACGAGGG GCAGAGGTCC 
TACCTGGTCC AGTACATGCA GTTCAGTGGC TCCCTCCATA CCAAACTCTA CCAGGAGGTG 
GAAATAGCCA GCGTGGACGC CTTTCAAGGC AGAGAGAAGG ACTTCATCAT CCTGTCCTGT 
GTCAGGGCCA ATGAGCACCA GGGCATCGGC TTCCTCAATG ATCCCAGACG TCTCAATGTG 
GCGCTCACTA GGGCCAGGTA CGGTGTGATC ATTGTGGGAA ACCCGAAAGC TCTGTCCAAG 
CAGCCTCTGT GGAACCACCT GCTGAACTAC TACAAGGAGC AGAAGGTCCT GGTCGAAGGT 
CCCCTTAACA ACCTGAGAGA GAGCCTCATG CAGTTCAGCA AGCCTCGCAA GCTAGTCAAC 
ACCATCAACC CCGGCGGTCG ATTTATGAGC ACAGCCATGT ATGACGCCAG GGAGGCCCTC 
ATTCCTGGAT CTGTTTATGA CCGCAGCAGC AACGGACGTA CGTCCAATAT GTATTTTCAG 
ACCCACGACC AGATCGGTAT GATTGGCGCA GGCACAAATC CCATGAACTC CATGAACATC 
CCCATCCCCT TCAACCTTGT AATGCCCCCC ATACCTCCAC CTGGTTACAT GGGACAGGTC 
AATGGACCCA CATCAGGTCG TGGTGGAGGT ATGAAAGGCA AGGCAGGTAG CGGAGGAGGA 
GGTGGGACTC GAGGTGGCCG TCAAAGAAAC CGCGGCAACA TGGGGAATCA CAGTGGAGGA 
GGAGACCGCC ACATGAGTGG CAGCCAGGCC AGCCAGGACC TGGGCTCCCA GCCTTTCTCC 
CAGGGGCCTC TGACGCAGGG CTACATCAAC ATGAGCCAGC CATCACAGAT GAGCCAGCCT 
GGGCTCTCCC AGCCTGAACT CTCACAGGAC AGTTACCTAG GAGACGAGTT CAAGTCCCAG 
ATTGATGTGG CTTTGTCCCA GGACTCCACC TACCAGGGGG AGCGGGCGTA CCAACACGGT 
GGTGTGACTG GACTGTCCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019115575.1
CDS	1..3327
Translation	MSVEAYGPSSQTLTFLDTEETELLGADTQGSEYDFTDFTLPSQTQTQGQTQSQLDGQVNGPDGVLQNGDDPVVKASQLLAELNFEEDEEDTYYTKDLPSHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKSKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPTATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPPWKGIGHVIKVPDNYGDEIAIELRSSAGAPVEIPHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVVIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQTRNMDSMPELQKLQQLKDETGELSSSDEKRYRALRRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGGRFMSTAMYDAREALIPGSVYDRSSNGRTSNMYFQTHDQIGMIGAGTNPMNSMNIPIPFNLVMPPIPPPGYMGQVNGPTSGRGGGMKGKAGSGGGGGTRGGRQRNRGNMGNHSGGGDRHMSGSQASQDLGSQPFSQGPLTQGYINMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_019260030.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea regulator of nonsense transcripts 1 (LOC104920975), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019260030.2

ORF Insert Sequence:

ATGAGTGTCG AGGCGTACGG GCCGAGCTCG CAGACTCTCA CGTTCCTCGA CACCGAGGAG 
ACCGAGCTGC TGGGAGCGGA CACCCAGGGC TCCGAGTACG ACTTCACCGA CTTCACCCTG 
CCGAGCCAGA CCCAGACCCA AGGCCAGACC CAGAGCCAGC TGGACGGACA GGTTAATGGT 
CCTGATGGTG TGCTGCAGAA TGGAGACGAC CCTGTGGTGA AAGCCAGTCA GCTGCTGGCA 
GAGTTGAACT TTGAGGAAGA TGAGGAGGAC ACCTACTACA CCAAGGACCT ACCTTCACAC 
GCCTGCAGTT ACTGTGGGAT TCATGATCCA GCCTGCGTGG TCTACTGCAA CACCAGCAAG 
AAGTGGTTCT GTAATGGCCG TGGAAACACG TCCGGAAGCC ACATTGTAAA CCACCTGGTG 
AGAGCAAAGT CCAAAGAGGT GACCCTACAT AAAGATGGCC CTCTGGGGGA AACTGTATTG 
GAGTGCTACA ACTGTGGCTG TCGCAATGTC TTCCTTCTGG GCTTCATCCC TGCCAAAGCT 
GACTCAGTAG TGGTGTTACT GTGCAGGCAG CCATGTGCGA GCCAGAGCAG CCTGAAGGAC 
ATCAACTGGG ACAGCTCACA GTGGCAGCCT CTCATCCAGG ACCGCTGCTT TCTGTCCTGG 
CTCGTCAAAA TCCCTTCAGA GCAGGAGCAG CTGAGGGCTC GTCAAATCAC CGCCCAGCAG 
ATCAACAAGC TGGAGGAACT CTGGAAGGAA AATCCCACTG CCACTTTGGA GGATCTGGAG 
AAGCCTGGTG TGGACGAGGA GCCTCAACAT GTGCTGCTCC GCTATGAGGA TGCCTACCAG 
TACCAGAACA TCTTTGGTCC TCTTGTGAAG CTGGAGGCTG ACTATGATAA GAAACTCAAA 
GAGTCTCAGA CCCAAGACAA CATCACAGTC AGATGGGACT TGGGCCTGAA TAAAAAGAGG 
ATTGCTTATT TCACTCTTCC CAAGACAGAC TCTGACATGC GACTGATGCA GGGAGATGAG 
ATTTGCCTGA GGTACAAAGG AGACCTGGCC CCTCCGTGGA AAGGCATTGG ACATGTCATC 
AAAGTCCCTG ATAACTATGG TGATGAGATT GCCATAGAGC TGAGGAGCAG TGCCGGCGCT 
CCAGTGGAGA TCCCACACAA CTTTCAGGTT GACTTTGTGT GGAAGTCCAC TTCCTTTGAC 
AGGATGCAAA GTGCGCTAAA GACATTTGCT GTGGATGAGA CTTCAGTGTC GGGCTACATC 
TACCACAAAC TGCTCGGACA TGAGGTTGAG GATGTGGTCA TCAAGTGCCA GCTGCCCAAA 
CGCTTCACTG CTCAAGGCCT GCCTGACCTT AACCACTCAC AGGTGTATGC TGTGAAGACA 
GTCCTGCAGC GTCCCCTCAG TCTGATCCAG GGTCCTCCTG GCACCGGGAA GACTGTAACC 
TCTGCGACCA TCGTCTACCA CCTGGCTAGA CAGGGCAACG GGCCTGTGCT GGTGTGTGCT 
CCCAGTAACA TCGCTGTCGA CCAGCTGACA GAAAAGATCC ACCAGACAGG CCTCAAGGTG 
GTGAGGCTGT GTGCCAAGAG CAGAGAGGCC ATCGACTCAC CGGTTTCCTT CCTGGCTCTG 
CACAACCAGA CCCGTAACAT GGACAGTATG CCCGAACTTC AGAAGCTCCA GCAGCTGAAG 
GACGAGACTG GAGAGCTGTC CTCTTCGGAC GAGAAGCGCT ACAGAGCTCT GAGACGCACT 
GCTGAGAGGG AGCTGCTCAT GAATGCCGAT GTGATCTGCT GTACCTGTGT TGGGGCGGGT 
GATCCACGTC TGGCCAAGAT GCAGTTCCGC TCAATCCTGA TCGATGAAAG CACCCAGGCC 
ACCGAACCGG AGTGCATGGT GCCTGTTGTC CTCGGGGCTA AACAGTTGAT TCTGGTGGGT 
GATCACTGCC AGCTGGGCCC CGTGGTGATG TGTAAGAAGG CAGCTAAAGC AGGTCTGTCT 
CAGTCTCTGT TTGAGCGTCT GGTAGTTTTG GGGATCCGGC CAATCCGTCT GCAGGTCCAG 
TACCGCATGC ACCCGGCACT CAGCGCCTTC CCTTCCAACA TCTTCTACGA GGGCTCTTTG 
CAGAACGGAG TCACTGCCGC GGACCGTGTG AAAAAAGGCT TCGACTTCCA GTGGCCACAG 
CCTGACAAGC CGATGTTCTT TTATGTTACC CAAGGCCAAG AGGAAATTGC CAGCTCTGGA 
ACATCCTATC TCAACAGGAC GGAGGCTGCC AATGTGGAGA AAATAACCAC AAGGTTGCTG 
AAGGCCGGAG CAAAACCTGA TCAGATCGGT ATCATCACAC CCTACGAGGG GCAGAGGTCC 
TACCTGGTCC AGTACATGCA GTTCAGTGGC TCCCTCCATA CCAAACTCTA CCAGGAGGTG 
GAAATAGCCA GCGTGGACGC CTTTCAAGGC AGAGAGAAGG ACTTCATCAT CCTGTCCTGT 
GTCAGGGCCA ATGAGCACCA GGGCATCGGC TTCCTCAATG ATCCCAGACG TCTCAATGTG 
GCGCTCACTA GGGCCAGGTA CGGTGTGATC ATTGTGGGAA ACCCGAAAGC TCTGTCCAAG 
CAGCCTCTGT GGAACCACCT GCTGAACTAC TACAAGGAGC AGAAGGTCCT GGTCGAAGGT 
CCCCTTAACA ACCTGAGAGA GAGCCTCATG CAGTTCAGCA AGCCTCGCAA GCTAGTCAAC 
ACCATCAACC CCGGCGGTCG ATTTATGAGC ACAGCCATGT ATGACGCCAG GGAGGCCCTC 
ATTCCTGGAT CTGTTTATGA CCGCAGCAGC AACGGACGTA CGTCCAATAT GTATTTTCAG 
ACCCACGACC AGATCGGTAT GATTGGCGCA GGCACAAATC CCATGAACTC CATGAACATC 
CCCATCCCCT TCAACCTTGT AATGCCCCCC ATACCTCCAC CTGGTTACAT GGGACAGGTC 
AATGGACCCA CATCAGGTCG TGGTGGAGGT ATGAAAGGCA AGGCAGGTAG CGGAGGAGGA 
GGTGGGACTC GAGGTGGCCG TCAAAGAAAC CGCGGCAACA TGGGGAATCA CAGTGGAGGA 
GGAGACCGCC ACATGAGTGG CAGCCAGGCC AGCCAGGACC TGGGCTCCCA GCCTTTCTCC 
CAGGGGCCTC TGACGCAGGG CTACATCAAC ATGAGCCAGC CATCACAGAT GAGCCAGCCT 
GGGCTCTCCC AGCCTGAACT CTCACAGGAC AGTTACCTAG GAGACGAGTT CAAGTCCCAG 
ATTGATGTGG CTTTGTCCCA GGACTCCACC TACCAGGGGG AGCGGGCGTA CCAACACGGT 
GGTGTGACTG GACTGTCCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104920975 ( XM_019260030.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019115575.1 Larimichthys crocea regulator of nonsense transcripts 1 (LOC104920975), mRNA.

Price & Availability↑

CloneID	OLa90792
Clone ID Related Accession (Same CDS sequence)	XM_019260030.1
Accession Version	XM_019260030.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	regulator of nonsense transcripts 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608313.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTCG AGGCGTACGG GCCGAGCTCG CAGACTCTCA CGTTCCTCGA CACCGAGGAG 
ACCGAGCTGC TGGGAGCGGA CACCCAGGGC TCCGAGTACG ACTTCACCGA CTTCACCCTG 
CCGAGCCAGA CCCAGACCCA AGGCCAGACC CAGAGCCAGC TGGACGGACA GGTTAATGGT 
CCTGATGGTG TGCTGCAGAA TGGAGACGAC CCTGTGGTGA AAGCCAGTCA GCTGCTGGCA 
GAGTTGAACT TTGAGGAAGA TGAGGAGGAC ACCTACTACA CCAAGGACCT ACCTTCACAC 
GCCTGCAGTT ACTGTGGGAT TCATGATCCA GCCTGCGTGG TCTACTGCAA CACCAGCAAG 
AAGTGGTTCT GTAATGGCCG TGGAAACACG TCCGGAAGCC ACATTGTAAA CCACCTGGTG 
AGAGCAAAGT CCAAAGAGGT GACCCTACAT AAAGATGGCC CTCTGGGGGA AACTGTATTG 
GAGTGCTACA ACTGTGGCTG TCGCAATGTC TTCCTTCTGG GCTTCATCCC TGCCAAAGCT 
GACTCAGTAG TGGTGTTACT GTGCAGGCAG CCATGTGCGA GCCAGAGCAG CCTGAAGGAC 
ATCAACTGGG ACAGCTCACA GTGGCAGCCT CTCATCCAGG ACCGCTGCTT TCTGTCCTGG 
CTCGTCAAAA TCCCTTCAGA GCAGGAGCAG CTGAGGGCTC GTCAAATCAC CGCCCAGCAG 
ATCAACAAGC TGGAGGAACT CTGGAAGGAA AATCCCACTG CCACTTTGGA GGATCTGGAG 
AAGCCTGGTG TGGACGAGGA GCCTCAACAT GTGCTGCTCC GCTATGAGGA TGCCTACCAG 
TACCAGAACA TCTTTGGTCC TCTTGTGAAG CTGGAGGCTG ACTATGATAA GAAACTCAAA 
GAGTCTCAGA CCCAAGACAA CATCACAGTC AGATGGGACT TGGGCCTGAA TAAAAAGAGG 
ATTGCTTATT TCACTCTTCC CAAGACAGAC TCTGACATGC GACTGATGCA GGGAGATGAG 
ATTTGCCTGA GGTACAAAGG AGACCTGGCC CCTCCGTGGA AAGGCATTGG ACATGTCATC 
AAAGTCCCTG ATAACTATGG TGATGAGATT GCCATAGAGC TGAGGAGCAG TGCCGGCGCT 
CCAGTGGAGA TCCCACACAA CTTTCAGGTT GACTTTGTGT GGAAGTCCAC TTCCTTTGAC 
AGGATGCAAA GTGCCCTAAA GACATTTGCT GTGGATGAGA CTTCAGTGTC GGGCTACATC 
TACCACAAAC TGCTCGGACA TGAGGTTGAG GATGTGGTCA TCAAGTGCCA GCTGCCCAAA 
CGCTTCACTG CTCAAGGCCT GCCTGACCTT AACCACTCAC AGGTGTATGC TGTGAAGACA 
GTGCTGCAGC GTCCCCTCAG TCTGATCCAG GGTCCTCCTG GCACCGGGAA GACTGTAACC 
TCTGCGACCA TCGTCTACCA CCTGGCTAGA CAGGGCAACG GGCCTGTGCT GGTGTGTGCT 
CCCAGTAACA TCGCTGTCGA CCAGCTGACA GAAAAGATCC ACCAGACAGG CCTCAAGGTG 
GTGAGGCTGT GTGCCAAGAG CAGAGAGGCC ATCGACTCAC CGGTTTCCTT CCTGGCTCTG 
CACAACCAGA CCCGTAACAT GGACAGTATG CCCGAACTTC AGAAGCTCCA GCAGCTGAAG 
GACGAGACTG GAGAGCTGTC CTCTTCGGAC GAGAAGCGCT ACAGAGCTCT GAGACGCACT 
GCTGAGAGAG AGCTGCTCAT GAATGCCGAT GTGATCTGCT GTACCTGTGT TGGGGCGGGT 
GATCCACGTC TGGCCAAGAT GCAGTTCCGC TCAATCCTGA TCGATGAAAG CACCCAGGCC 
ACCGAACCGG AGTGCATGGT GCCTGTTGTC CTCGGGGCTA AACAGTTGAT TCTGGTGGGT 
GATCACTGCC AGCTGGGCCC CGTGGTGATG TGTAAGAAGG CAGCTAAAGC AGGTCTGTCT 
CAGTCTCTGT TTGAGCGTCT GGTAGTTTTG GGGATCCGGC CAATCCGTCT GCAGGTCCAG 
TACCGCATGC ACCCGGCACT CAGCGCCTTC CCTTCCAACA TCTTCTACGA GGGCTCTTTG 
CAGAACGGAG TCACTGCCGC GGACCGTGTG AAAAAAGGCT TCGACTTCCA GTGGCCACAG 
CCTGACAAGC CGATGTTCTT TTATGTTACC CAAGGCCAAG AGGAAATTGC CAGCTCTGGA 
ACATCCTATC TCAACAGGAC GGAGGCTGCC AATGTGGAGA AAATAACCAC AAGGTTGCTG 
AAGGCCGGAG CAAAACCTGA TCAGATCGGT ATCATCACAC CCTACGAGGG GCAGAGGTCC 
TACCTGGTCC AGTACATGCA GTTCAGTGGC TCCCTCCATA CCAAACTCTA CCAGGAGGTG 
GAAATAGCCA GCGTGGACGC CTTTCAAGGC AGAGAGAAGG ACTTCATCAT CCTGTCCTGT 
GTCAGGGCCA ATGAGCACCA GGGCATCGGC TTCCTCAATG ATCCCAGACG TCTCAATGTG 
GCGCTCACTA GGGCCAGGTA CGGTGTGATC ATTGTGGGAA ACCCGAAAGC TCTGTCCAAG 
CAGCCTCTGT GGAACCACCT GCTGAACTAC TACAAGGAGC AGAAGGTCCT GGTCGAAGGT 
CCCCTTAACA ACCTGAGAGA GAGCCTCATG CAGTTCAGCA AGCCTCGCAA GCTAGTCAAC 
ACCATCAACC CTGGCGGTCG ATTTATGAGC ACAGCCATGT ATGACGCCAG GGAGGCCCTC 
ATTCCTGGAT CTGTTTATGA CCGCAGCAGC AACGGACGTA CGTCCAATAT GTATTTTCAG 
ACCCACGACC AGATCGGTAT GATTGGCGCA GGCACAAATC CCATGAACTC CATGAACATC 
CCCATCCCCT TCAACCTTGT AATGCCCCCC ATACCTCCAC CTGGTTACAT GGGACAGGTC 
AATGGACCCA CATCAGGTCG TGGTGGAGGT ATGAAAGGCA AGGCAGGTAG CGGAGGAGGA 
GGCGGGACTC GAGGTGGCCG TCAAAGAAAC CGCGGCAACA TGGGGAATCA CAGTGGAGGA 
GGAGACCGCC ACATGAGTGG CAGCCAGGCC AGCCAGGACC TGGGCTCCCA GCCTTTCTCC 
CAGGGGCCTC TGACGCAGGG CTACATCAAC ATGAGCCAGC CATCACAGAT GAGCCAGCCT 
GGGCTCTCCC AGCCTGAACT CTCACAGGAC AGTTACCTAG GAGACGAGTT CAAGTCCCAG 
ATTGATGTGG CTTTGTCCCA GGACTCCACC TACCAGGGGG AGCGGGCGTA CCAACACGGT 
GGTGTGACTG GACTGTCCCA GTACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019115575.1
CDS	195..3521
Translation	MSVEAYGPSSQTLTFLDTEETELLGADTQGSEYDFTDFTLPSQTQTQGQTQSQLDGQVNGPDGVLQNGDDPVVKASQLLAELNFEEDEEDTYYTKDLPSHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKSKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPTATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPPWKGIGHVIKVPDNYGDEIAIELRSSAGAPVEIPHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVVIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQTRNMDSMPELQKLQQLKDETGELSSSDEKRYRALRRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTRLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGGRFMSTAMYDAREALIPGSVYDRSSNGRTSNMYFQTHDQIGMIGAGTNPMNSMNIPIPFNLVMPPIPPPGYMGQVNGPTSGRGGGMKGKAGSGGGGGTRGGRQRNRGNMGNHSGGGDRHMSGSQASQDLGSQPFSQGPLTQGYINMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_019260030.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea regulator of nonsense transcripts 1 (LOC104920975), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019260030.1

ORF Insert Sequence:

ATGAGTGTCG AGGCGTACGG GCCGAGCTCG CAGACTCTCA CGTTCCTCGA CACCGAGGAG 
ACCGAGCTGC TGGGAGCGGA CACCCAGGGC TCCGAGTACG ACTTCACCGA CTTCACCCTG 
CCGAGCCAGA CCCAGACCCA AGGCCAGACC CAGAGCCAGC TGGACGGACA GGTTAATGGT 
CCTGATGGTG TGCTGCAGAA TGGAGACGAC CCTGTGGTGA AAGCCAGTCA GCTGCTGGCA 
GAGTTGAACT TTGAGGAAGA TGAGGAGGAC ACCTACTACA CCAAGGACCT ACCTTCACAC 
GCCTGCAGTT ACTGTGGGAT TCATGATCCA GCCTGCGTGG TCTACTGCAA CACCAGCAAG 
AAGTGGTTCT GTAATGGCCG TGGAAACACG TCCGGAAGCC ACATTGTAAA CCACCTGGTG 
AGAGCAAAGT CCAAAGAGGT GACCCTACAT AAAGATGGCC CTCTGGGGGA AACTGTATTG 
GAGTGCTACA ACTGTGGCTG TCGCAATGTC TTCCTTCTGG GCTTCATCCC TGCCAAAGCT 
GACTCAGTAG TGGTGTTACT GTGCAGGCAG CCATGTGCGA GCCAGAGCAG CCTGAAGGAC 
ATCAACTGGG ACAGCTCACA GTGGCAGCCT CTCATCCAGG ACCGCTGCTT TCTGTCCTGG 
CTCGTCAAAA TCCCTTCAGA GCAGGAGCAG CTGAGGGCTC GTCAAATCAC CGCCCAGCAG 
ATCAACAAGC TGGAGGAACT CTGGAAGGAA AATCCCACTG CCACTTTGGA GGATCTGGAG 
AAGCCTGGTG TGGACGAGGA GCCTCAACAT GTGCTGCTCC GCTATGAGGA TGCCTACCAG 
TACCAGAACA TCTTTGGTCC TCTTGTGAAG CTGGAGGCTG ACTATGATAA GAAACTCAAA 
GAGTCTCAGA CCCAAGACAA CATCACAGTC AGATGGGACT TGGGCCTGAA TAAAAAGAGG 
ATTGCTTATT TCACTCTTCC CAAGACAGAC TCTGACATGC GACTGATGCA GGGAGATGAG 
ATTTGCCTGA GGTACAAAGG AGACCTGGCC CCTCCGTGGA AAGGCATTGG ACATGTCATC 
AAAGTCCCTG ATAACTATGG TGATGAGATT GCCATAGAGC TGAGGAGCAG TGCCGGCGCT 
CCAGTGGAGA TCCCACACAA CTTTCAGGTT GACTTTGTGT GGAAGTCCAC TTCCTTTGAC 
AGGATGCAAA GTGCCCTAAA GACATTTGCT GTGGATGAGA CTTCAGTGTC GGGCTACATC 
TACCACAAAC TGCTCGGACA TGAGGTTGAG GATGTGGTCA TCAAGTGCCA GCTGCCCAAA 
CGCTTCACTG CTCAAGGCCT GCCTGACCTT AACCACTCAC AGGTGTATGC TGTGAAGACA 
GTGCTGCAGC GTCCCCTCAG TCTGATCCAG GGTCCTCCTG GCACCGGGAA GACTGTAACC 
TCTGCGACCA TCGTCTACCA CCTGGCTAGA CAGGGCAACG GGCCTGTGCT GGTGTGTGCT 
CCCAGTAACA TCGCTGTCGA CCAGCTGACA GAAAAGATCC ACCAGACAGG CCTCAAGGTG 
GTGAGGCTGT GTGCCAAGAG CAGAGAGGCC ATCGACTCAC CGGTTTCCTT CCTGGCTCTG 
CACAACCAGA CCCGTAACAT GGACAGTATG CCCGAACTTC AGAAGCTCCA GCAGCTGAAG 
GACGAGACTG GAGAGCTGTC CTCTTCGGAC GAGAAGCGCT ACAGAGCTCT GAGACGCACT 
GCTGAGAGAG AGCTGCTCAT GAATGCCGAT GTGATCTGCT GTACCTGTGT TGGGGCGGGT 
GATCCACGTC TGGCCAAGAT GCAGTTCCGC TCAATCCTGA TCGATGAAAG CACCCAGGCC 
ACCGAACCGG AGTGCATGGT GCCTGTTGTC CTCGGGGCTA AACAGTTGAT TCTGGTGGGT 
GATCACTGCC AGCTGGGCCC CGTGGTGATG TGTAAGAAGG CAGCTAAAGC AGGTCTGTCT 
CAGTCTCTGT TTGAGCGTCT GGTAGTTTTG GGGATCCGGC CAATCCGTCT GCAGGTCCAG 
TACCGCATGC ACCCGGCACT CAGCGCCTTC CCTTCCAACA TCTTCTACGA GGGCTCTTTG 
CAGAACGGAG TCACTGCCGC GGACCGTGTG AAAAAAGGCT TCGACTTCCA GTGGCCACAG 
CCTGACAAGC CGATGTTCTT TTATGTTACC CAAGGCCAAG AGGAAATTGC CAGCTCTGGA 
ACATCCTATC TCAACAGGAC GGAGGCTGCC AATGTGGAGA AAATAACCAC AAGGTTGCTG 
AAGGCCGGAG CAAAACCTGA TCAGATCGGT ATCATCACAC CCTACGAGGG GCAGAGGTCC 
TACCTGGTCC AGTACATGCA GTTCAGTGGC TCCCTCCATA CCAAACTCTA CCAGGAGGTG 
GAAATAGCCA GCGTGGACGC CTTTCAAGGC AGAGAGAAGG ACTTCATCAT CCTGTCCTGT 
GTCAGGGCCA ATGAGCACCA GGGCATCGGC TTCCTCAATG ATCCCAGACG TCTCAATGTG 
GCGCTCACTA GGGCCAGGTA CGGTGTGATC ATTGTGGGAA ACCCGAAAGC TCTGTCCAAG 
CAGCCTCTGT GGAACCACCT GCTGAACTAC TACAAGGAGC AGAAGGTCCT GGTCGAAGGT 
CCCCTTAACA ACCTGAGAGA GAGCCTCATG CAGTTCAGCA AGCCTCGCAA GCTAGTCAAC 
ACCATCAACC CTGGCGGTCG ATTTATGAGC ACAGCCATGT ATGACGCCAG GGAGGCCCTC 
ATTCCTGGAT CTGTTTATGA CCGCAGCAGC AACGGACGTA CGTCCAATAT GTATTTTCAG 
ACCCACGACC AGATCGGTAT GATTGGCGCA GGCACAAATC CCATGAACTC CATGAACATC 
CCCATCCCCT TCAACCTTGT AATGCCCCCC ATACCTCCAC CTGGTTACAT GGGACAGGTC 
AATGGACCCA CATCAGGTCG TGGTGGAGGT ATGAAAGGCA AGGCAGGTAG CGGAGGAGGA 
GGCGGGACTC GAGGTGGCCG TCAAAGAAAC CGCGGCAACA TGGGGAATCA CAGTGGAGGA 
GGAGACCGCC ACATGAGTGG CAGCCAGGCC AGCCAGGACC TGGGCTCCCA GCCTTTCTCC 
CAGGGGCCTC TGACGCAGGG CTACATCAAC ATGAGCCAGC CATCACAGAT GAGCCAGCCT 
GGGCTCTCCC AGCCTGAACT CTCACAGGAC AGTTACCTAG GAGACGAGTT CAAGTCCCAG 
ATTGATGTGG CTTTGTCCCA GGACTCCACC TACCAGGGGG AGCGGGCGTA CCAACACGGT 
GGTGTGACTG GACTGTCCCA GTACTAG