gemin4 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	gemin4
Entrez Gene ID	104920869
Full Name	gem nuclear organelle associated protein 4
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010733251.3	XP_010731553.3	gem-associated protein 4
XM_010733251.2	XP_010731553.2	gem-associated protein 4

Pathways from BioSystems
Gene Ontology

The following gemin4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gemin4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa100810	XM_010733251.2 Latest version!	Larimichthys crocea gem nuclear organelle associated protein 4 (gemin4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OLa222367	XM_010733251.3 Latest version!	Larimichthys crocea gem nuclear organelle associated protein 4 (gemin4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gemin4 ( XM_010733251.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010731553.2 Larimichthys crocea gem nuclear organelle associated protein 4 (gemin4), mRNA.

Price & Availability↑

CloneID	OLa100810
Clone ID Related Accession (Same CDS sequence)	XM_010733251.2
Accession Version	XM_010733251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608919.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 29, 2016 this sequence version replaced XM_010733251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACA GAGACTCGGC CGTCCTTCAG GGGGCTTTCC TCCTGGCTGA TCAGCTGTGT 
CTCCCCTCCT CCCTGTCCTC CCTTCAGAAA GCTGACTGGA GCAGGGTGGG ACGTCCAGTC 
CTGGAGGCAC TTGGAGAAAT CTGTAGACTC GGTGCACACC CCACGACGAC AACTATCAGC 
TGGACGAAGA AAGTCATTTG CGTTGTGTGG CTGAAGCTGC TGTGCAGGGA GACTGGAGAG 
GACCTGGAGA CGGGCTGGAG GGAGAACCCC TTTTTCCCCC TCCAGAACGG CCTCCCTGAC 
GTCAACTCCG CCGTCCTGCT GGAAGTGGTG AAGTCGACGG CGGCCGCTCA GACGTTTGCC 
CATTTCCTCT TGTGTCTTCC TCAGTCTCAG ATCTGTGCCG AGCTCGAGAG GCTGGTGCAG 
CACGTGAGGA GCAGCTCCAC CGCAGAGGAC GATGTCCGGC TTCTCCTGGA GGTGTGGTGG 
GAGCTGTGGA AAGGCAGAGA TGAGCAGAAA GCCGGAGAAG GGGAGAGCAT AGAGGCGATG 
TTTGCTCGAC AATACACTCG TCTCCAGTCT TCCAGTGTCC CCCCACAGGC TGCCAAGAGG 
TTAAAACTGG ACCCATCAGA ACTGAAGGCA TCGTCTCCAA CCTCAGATGT CCTGCACATT 
CTGCTTCATG CACTGAAAGA CATTAAAGAT CACATAACTA CAACAGAGCT TTGTCTGCAG 
GCCCTCTCGG TTTCTCTTGA TGCTCTTTAC ACATCTTGCC TGATAGACCA GGCAGTCGCA 
CTTCCACCCA AAGAGAAGAT CCGCGTCCTG TCTGTAATTG TCAGTATCAG AGAAAGGAAC 
AACGAGAAAC TGAGTCCTGA ACTTATTCAG GAGGCCGTGA GAGACCTGCA CGCTTCTCAC 
ACACCATCTC AGTTTCGATA CAGCAGGATG AAGCTTGGCG AAGCCTTGAA AAATATAACG 
GAACTGGCTC GGTTTTGGCA AAGCAGCGAG CTCCTCAAAG TGTGTGACAG CACTAATCCG 
AGCTACTCAG CATTCAGACT GGAACAGAGT GTCCGTAGAG TCCTTGCAGC TCTACAGAAA 
GTGCCTGAAG CTATGACTGA AACAGACGAT CAAGAGGCTG AGAAGGACAC GCTGAGAGGT 
TTGCTAGAGT CACTGGCTCT CCCTGTCACA GAGAGCACCC CAGAGGTGAG CGCGAGTGTC 
ACCGCGATCA TCATCAGCCA CCGCCTGGAC GACTATCAGA ACTTTGCGGC GTTATTTGCC 
AGCGAGGAAA GCTGGGCTGC CTGCGAACAG CACTGGATGG ACTGCCTGGA AAAGAACCAA 
GCAGCCTTCC AGCAGTGCGA CACGCTCATC AGACTGACCT CCACCCTCAT GCGTAAACTC 
CAGAGCGAGA GCGCCAACGT GAGCCACTGC AAGAAGCTGA TGAAAGTCAT CACAGACATT 
TTCTCTGCAC TTTCTTTAAA AGACAAGAAC ACAGCGTTGG CTGCTATGTT AAGGCTGTCC 
AGCAGGGGGT TCTTCGGACG CTGCGTCCCC TCTGCTGTGA CTGAAGGGTT CGAACAGGAG 
CTCAGCATGG CCTTCAACTG CATCATCCAA GGACGGGGTG GAGCGTCAGC AGCGGCGCTG 
CAGGGCAATC TGAACACGGC GGCTTCTTTG GTGGCGAGAG TTGCGTTTCA GAACCCGGAG 
GCCGCTCTGA GGTCTTGCTG TCATTCAGCT ATTTTCAACA AAGGTGCCTT CTCTCTCATG 
GCGAAGATCC TGCAGCAGCT GCCTGGACTC AGGGGCGAAG AGGAAAGTAT AGATGAAGGA 
AATGATGCTC TGAGTGGTGG TAGTCTGCTC TGCAGGTGTC TACAGGAGAT CATCAACACC 
AAAGCACTGT CAGCCAATGA AAAAGAGCAG TTCCTCAAGT ACGTGGGTCT GCTGATGAGG 
CCTGTCATGA CAGGCGAGGT AGAAGGAAGA AGGCGAAGCT TTCTGCCACC TCAGGAGGTC 
GTGAACATCT TTGTCCTGCC GAATCTCTCC ACCACGGGTC AAAGCTCCTT CGACATAGAG 
CTGAGTCTGC AGCTCCTCCA CACTGCTCTG TCTGTGGACG TCCAGGAACC AGACGGGTCG 
CCTCACTGGG TGTTGGACTG CTCTCCCTTT CCCCTCCTCT ATGTCCTGGC TCAGTTCCAC 
AGCCAGACTC TCAGTTGTTG GGAGCAGCCA CCAGATGGTG CTGTCTATCA CTGGTCAATG 
GACCTGAAGG AGCTGCTGGT GTCTGTGCTG ACCACCCTGG GGAACGTGGT GGGTGCAGAG 
GTGGCGGCGG TCCCCAGCAG CTGGTCCAGA GCTCTGTTCT GGCTTTACAA GAAAATGGAG 
GATCTGGACT GGACCGTTCG TTTCCACCTG AAGGCTGTTT GGGGGGAACA CTTTAAAAAC 
GAGGTTCCCT CGTCCCTGCT GGCTGTGTGT GATCTACCTG AGCAGGAGTG GTCCAGTTTG 
GACCTGCCTC AGTATGGTCA GGGTACAGGT CTTCTGGCCT GGATGGAGTG TTGTTCCATA 
TCCGACTCCC TGCAGTCCAC CATGCTGTCC CGTCTCTCTC TGGATCAGCG TCAGCTCGAC 
CACGTCAGTA TGTTCAGCAA AGGCCTGCTG GTGGCTCTGG CCCAGATCCT GCCCTGGTGC 
TCCATCTCCC AGTGGACCAG GCTGCTGAGA GCCCTGAGGG AGCTGATCAC CTCAGGCCGC 
CTCCATGTGC CCTTCTCACT CGAGTATGTG GACTACCTGC CCCTGCTGGA TCTAAGGAGG 
TTTTCATGTG AGCTCCGCCT GTCTGTCCTC CTGCTTCGAG TCCTTCAGCT GCTCTGCGGG 
TCCAGCTGCT CCGACTGGCT GTCTGCGGAC GGCTGGGCAC ACGTCGGCAG GTTATATTCC 
CACGCTGTGA GGGAGATGAT AAACTCAGTA AGAGACAAGC TGCCTCTTCC TTCATCCGGT 
GCTTTAACGT CTCCTAAAAC ACCAGCGTCT AAAGACTCTG ACCCATCGTG CACTTCCATC 
ACATCTCCTA AAATGTCCAA AGACTCTCAA AAAGACCTGA ATCAAGCAGA CGGCTCACAG 
GTGGAGGAGG AGGTGGGGAC AGTTCCCAGC CAGGAAGTCC TTTTTGTGCT CAGCCAGCTC 
TTTTGCCATG TTCAGCACAT TCAGGTGATG ATGCCAGGAG GTCAGTGTGA ACCACTCTTC 
CTGTCCAGTC TGGAGATCCT CAGCCACTAC GAGGCCATCA TGGCCGCTTT CCCCGACAGC 
AGCAGCCCGC TGGAGAGCGA CAACACCCGG CACTTCTTCT CCACCATCAC AGACAACCTG 
CAGAGCCAGG AGATGAAGTC TGTGCTGCAG CAGAAGATTG CTCAGCTCGT GTCAAAGGCA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010731553.2
CDS	237..3602
Translation	MMDRDSAVLQGAFLLADQLCLPSSLSSLQKADWSRVGRPVLEALGEICRLGAHPTTTTISWTKKVICVVWLKLLCRETGEDLETGWRENPFFPLQNGLPDVNSAVLLEVVKSTAAAQTFAHFLLCLPQSQICAELERLVQHVRSSSTAEDDVRLLLEVWWELWKGRDEQKAGEGESIEAMFARQYTRLQSSSVPPQAAKRLKLDPSELKASSPTSDVLHILLHALKDIKDHITTTELCLQALSVSLDALYTSCLIDQAVALPPKEKIRVLSVIVSIRERNNEKLSPELIQEAVRDLHASHTPSQFRYSRMKLGEALKNITELARFWQSSELLKVCDSTNPSYSAFRLEQSVRRVLAALQKVPEAMTETDDQEAEKDTLRGLLESLALPVTESTPEVSASVTAIIISHRLDDYQNFAALFASEESWAACEQHWMDCLEKNQAAFQQCDTLIRLTSTLMRKLQSESANVSHCKKLMKVITDIFSALSLKDKNTALAAMLRLSSRGFFGRCVPSAVTEGFEQELSMAFNCIIQGRGGASAAALQGNLNTAASLVARVAFQNPEAALRSCCHSAIFNKGAFSLMAKILQQLPGLRGEEESIDEGNDALSGGSLLCRCLQEIINTKALSANEKEQFLKYVGLLMRPVMTGEVEGRRRSFLPPQEVVNIFVLPNLSTTGQSSFDIELSLQLLHTALSVDVQEPDGSPHWVLDCSPFPLLYVLAQFHSQTLSCWEQPPDGAVYHWSMDLKELLVSVLTTLGNVVGAEVAAVPSSWSRALFWLYKKMEDLDWTVRFHLKAVWGEHFKNEVPSSLLAVCDLPEQEWSSLDLPQYGQGTGLLAWMECCSISDSLQSTMLSRLSLDQRQLDHVSMFSKGLLVALAQILPWCSISQWTRLLRALRELITSGRLHVPFSLEYVDYLPLLDLRRFSCELRLSVLLLRVLQLLCGSSCSDWLSADGWAHVGRLYSHAVREMINSVRDKLPLPSSGALTSPKTPASKDSDPSCTSITSPKMSKDSQKDLNQADGSQVEEEVGTVPSQEVLFVLSQLFCHVQHIQVMMPGGQCEPLFLSSLEILSHYEAIMAAFPDSSSPLESDNTRHFFSTITDNLQSQEMKSVLQQKIAQLVSKAA

Target ORF information:

RefSeq Version	XM_010733251.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea gem nuclear organelle associated protein 4 (gemin4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010733251.2

ORF Insert Sequence:

ATGATGGACA GAGACTCGGC CGTCCTTCAG GGGGCTTTCC TCCTGGCTGA TCAGCTGTGT 
CTCCCCTCCT CCCTGTCCTC CCTTCAGAAA GCTGACTGGA GCAGGGTGGG ACGTCCAGTC 
CTGGAGGCAC TTGGAGAAAT CTGTAGACTC GGTGCACACC CCACGACGAC AACTATCAGC 
TGGACGAAGA AAGTCATTTG CGTTGTGTGG CTGAAGCTGC TGTGCAGGGA GACTGGAGAG 
GACCTGGAGA CGGGCTGGAG GGAGAACCCC TTTTTCCCCC TCCAGAACGG CCTCCCTGAC 
GTCAACTCCG CCGTCCTGCT GGAAGTGGTG AAGTCGACGG CGGCCGCTCA GACGTTTGCC 
CATTTCCTCT TGTGTCTTCC TCAGTCTCAG ATCTGTGCCG AGCTCGAGAG GCTGGTGCAG 
CACGTGAGGA GCAGCTCCAC CGCAGAGGAC GATGTCCGGC TTCTCCTGGA GGTGTGGTGG 
GAGCTGTGGA AAGGCAGAGA TGAGCAGAAA GCCGGAGAAG GGGAGAGCAT AGAGGCGATG 
TTTGCTCGAC AATACACTCG TCTCCAGTCT TCCAGTGTCC CCCCACAGGC TGCCAAGAGG 
TTAAAACTGG ACCCATCAGA ACTGAAGGCA TCGTCTCCAA CCTCAGATGT CCTGCACATT 
CTGCTTCATG CACTGAAAGA CATTAAAGAT CACATAACTA CAACAGAGCT TTGTCTGCAG 
GCCCTCTCGG TTTCTCTTGA TGCTCTTTAC ACATCTTGCC TGATAGACCA GGCAGTCGCA 
CTTCCACCCA AAGAGAAGAT CCGCGTCCTG TCTGTAATTG TCAGTATCAG AGAAAGGAAC 
AACGAGAAAC TGAGTCCTGA ACTTATTCAG GAGGCCGTGA GAGACCTGCA CGCTTCTCAC 
ACACCATCTC AGTTTCGATA CAGCAGGATG AAGCTTGGCG AAGCCTTGAA AAATATAACG 
GAACTGGCTC GGTTTTGGCA AAGCAGCGAG CTCCTCAAAG TGTGTGACAG CACTAATCCG 
AGCTACTCAG CATTCAGACT GGAACAGAGT GTCCGTAGAG TCCTTGCAGC TCTACAGAAA 
GTGCCTGAAG CTATGACTGA AACAGACGAT CAAGAGGCTG AGAAGGACAC GCTGAGAGGT 
TTGCTAGAGT CACTGGCTCT CCCTGTCACA GAGAGCACCC CAGAGGTGAG CGCGAGTGTC 
ACCGCGATCA TCATCAGCCA CCGCCTGGAC GACTATCAGA ACTTTGCGGC GTTATTTGCC 
AGCGAGGAAA GCTGGGCTGC CTGCGAACAG CACTGGATGG ACTGCCTGGA AAAGAACCAA 
GCAGCCTTCC AGCAGTGCGA CACGCTCATC AGACTGACCT CCACCCTCAT GCGTAAACTC 
CAGAGCGAGA GCGCCAACGT GAGCCACTGC AAGAAGCTGA TGAAAGTCAT CACAGACATT 
TTCTCTGCAC TTTCTTTAAA AGACAAGAAC ACAGCGTTGG CTGCTATGTT AAGGCTGTCC 
AGCAGGGGGT TCTTCGGACG CTGCGTCCCC TCTGCTGTGA CTGAAGGGTT CGAACAGGAG 
CTCAGCATGG CCTTCAACTG CATCATCCAA GGACGGGGTG GAGCGTCAGC AGCGGCGCTG 
CAGGGCAATC TGAACACGGC GGCTTCTTTG GTGGCGAGAG TTGCGTTTCA GAACCCGGAG 
GCCGCTCTGA GGTCTTGCTG TCATTCAGCT ATTTTCAACA AAGGTGCCTT CTCTCTCATG 
GCGAAGATCC TGCAGCAGCT GCCTGGACTC AGGGGCGAAG AGGAAAGTAT AGATGAAGGA 
AATGATGCTC TGAGTGGTGG TAGTCTGCTC TGCAGGTGTC TACAGGAGAT CATCAACACC 
AAAGCACTGT CAGCCAATGA AAAAGAGCAG TTCCTCAAGT ACGTGGGTCT GCTGATGAGG 
CCTGTCATGA CAGGCGAGGT AGAAGGAAGA AGGCGAAGCT TTCTGCCACC TCAGGAGGTC 
GTGAACATCT TTGTCCTGCC GAATCTCTCC ACCACGGGTC AAAGCTCCTT CGACATAGAG 
CTGAGTCTGC AGCTCCTCCA CACTGCTCTG TCTGTGGACG TCCAGGAACC AGACGGGTCG 
CCTCACTGGG TGTTGGACTG CTCTCCCTTT CCCCTCCTCT ATGTCCTGGC TCAGTTCCAC 
AGCCAGACTC TCAGTTGTTG GGAGCAGCCA CCAGATGGTG CTGTCTATCA CTGGTCAATG 
GACCTGAAGG AGCTGCTGGT GTCTGTGCTG ACCACCCTGG GGAACGTGGT GGGTGCAGAG 
GTGGCGGCGG TCCCCAGCAG CTGGTCCAGA GCTCTGTTCT GGCTTTACAA GAAAATGGAG 
GATCTGGACT GGACCGTTCG TTTCCACCTG AAGGCTGTTT GGGGGGAACA CTTTAAAAAC 
GAGGTTCCCT CGTCCCTGCT GGCTGTGTGT GATCTACCTG AGCAGGAGTG GTCCAGTTTG 
GACCTGCCTC AGTATGGTCA GGGTACAGGT CTTCTGGCCT GGATGGAGTG TTGTTCCATA 
TCCGACTCCC TGCAGTCCAC CATGCTGTCC CGTCTCTCTC TGGATCAGCG TCAGCTCGAC 
CACGTCAGTA TGTTCAGCAA AGGCCTGCTG GTGGCTCTGG CCCAGATCCT GCCCTGGTGC 
TCCATCTCCC AGTGGACCAG GCTGCTGAGA GCCCTGAGGG AGCTGATCAC CTCAGGCCGC 
CTCCATGTGC CCTTCTCACT CGAGTATGTG GACTACCTGC CCCTGCTGGA TCTAAGGAGG 
TTTTCATGTG AGCTCCGCCT GTCTGTCCTC CTGCTTCGAG TCCTTCAGCT GCTCTGCGGG 
TCCAGCTGCT CCGACTGGCT GTCTGCGGAC GGCTGGGCAC ACGTCGGCAG GTTATATTCC 
CACGCTGTGA GGGAGATGAT AAACTCAGTA AGAGACAAGC TGCCTCTTCC TTCATCCGGT 
GCTTTAACGT CTCCTAAAAC ACCAGCGTCT AAAGACTCTG ACCCATCGTG CACTTCCATC 
ACATCTCCTA AAATGTCCAA AGACTCTCAA AAAGACCTGA ATCAAGCAGA CGGCTCACAG 
GTGGAGGAGG AGGTGGGGAC AGTTCCCAGC CAGGAAGTCC TTTTTGTGCT CAGCCAGCTC 
TTTTGCCATG TTCAGCACAT TCAGGTGATG ATGCCAGGAG GTCAGTGTGA ACCACTCTTC 
CTGTCCAGTC TGGAGATCCT CAGCCACTAC GAGGCCATCA TGGCCGCTTT CCCCGACAGC 
AGCAGCCCGC TGGAGAGCGA CAACACCCGG CACTTCTTCT CCACCATCAC AGACAACCTG 
CAGAGCCAGG AGATGAAGTC TGTGCTGCAG CAGAAGATTG CTCAGCTCGT GTCAAAGGCA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gemin4 ( XM_010733251.3 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_010731553.3 Larimichthys crocea gem nuclear organelle associated protein 4 (gemin4), mRNA.

Price & Availability↑

CloneID	OLa222367
Clone ID Related Accession (Same CDS sequence)	XM_010733251.3
Accession Version	XM_010733251.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	gem-associated protein 4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040017.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_010733251.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACA GAGACTCGGC CGTCCTTCAG GGGGCTTTCC TCCTGGCTGA TCAGCTGTGT 
CTCCCCTCCT CCCTGTCCTC CCTTCAGAAA GCTGACTGGA GCAGGGTGGG ACGTCCAGTC 
CTGGAGGCAC TTGGAGAAAT CTGTAGACTC GGTGCACACC CCACGACGAC AACTATCAGC 
TGGACGAAGA AAGTCATTTG CGTTGTGTGG CTGAAGCTGC TGTGCAGGGA GACTGGAGAG 
GACCTGGAGA CGGGCTGGAG GGAGAACCCC TTTTTCCCCC TCCAGAACGG CCTCCCTGAC 
GTCAACTCCG CCGTCCTGCT GGAAGTGGTG AAGTCGACGG CGGCCGCTCA GACGTTTGCC 
CATTTCCTCT TGTGTCTTCC TCAGTCTCAG ATCTGTGCCG AGCTCGAGAG GCTGGTGCAG 
CACGTGAGGA GCAGCTCCAC CGCAGAGGAC GATGTCCGGC TTCTCCTGGA GGTGTGGTGG 
GAGCTGTGGA AAGGCAGAGA TGAGCAGAAA GCCGGAGAAG GGGAGAGCAT AGAGGCGATG 
TTTGCTCGAC AATACACTCG TCTCCAGTCT TCCAGTGTCC CCCCACAGGC TGCCAAGAGG 
TTAAAACTGG ACCCATCAGA ACTGAAGGCA TCGTCTCCAA CCTCAGATGT CCTGCACATT 
CTGCTTCATG CACTGAAAGA CATTAAAGAT CACATAACTA CAACAGAGCT TTGTCTGCAG 
GCCCTCTCGG TTTCTCTTGA TGCTCTTTAC ACATCTTGCC TGATAGACCA GGCAGTCGCA 
CTTCCACCCA AAGAGAAGAT CCGCGTCCTG TCTGTAATTG TCAGTATCAG AGAAAGGAAC 
AACGAGAAAC TGAGTCCTGA ACTTATTCAG GAGGCCGTGA GAGACCTGCA CGCTTCTCAC 
ACACCATCTC AGTTTCGATA CAGCAGGATG AAGCTTGGCG AAGCCTTGAA AAATATAACG 
GAACTGGCTC GGTTTTGGCA AAGCAGCGAG CTCCTCAAAG TGTGTGACAG CACTAATCCG 
AGCTACTCAG CATTCAGACT GGAACAGAGT GTCCGTAGAG TCCTTGCAGC TCTACAGAAA 
GTGCCTGAAG CTATGACTGA AACAGACGAT CAAGAGGCTG AGAAGGACAC GCTGAGAGGT 
TTGCTAGAGT CACTGGCTCT CCCTGTCACA GAGAGCACCC CAGAGGTGAG CGCGAGTGTC 
ACCGCGATCA TCATCAGCCA CCGCCTGGAC GACTATCAGA ACTTTGCGGC GTTATTTGCC 
AGCGAGGAAA GCTGGGCTGC CTGCGAACAG CACTGGATGG ACTGCCTGGA AAAGAACCAA 
GCAGCCTTCC AGCAGTGCGA CACGCTCATC AGACTGACCT CCACCCTCAT GCGTAAACTC 
CAGAGCGAGA GCGCCAACGT GAGCCACTGC AAGAAGCTGA TGAAAGTCAT CACAGACATT 
TTCTCTGCAC TTTCTTTAAA AGACAAGAAC ACAGCGTTGG CTGCTATGTT AAGGCTCTCC 
ATAAGGGGGT TCTTCGGACG CTGCGTCCCC TCTGCTGTGA CTGAAGGGTT CGAACAGGAG 
CTCAGCATGG CCTTCAACTG CATCATCCAA GGACGGGGTG GAGCGTCAGC AGCGGCGCTG 
CAGGGCAATC TGAACACGGC GGCTTCTTTG GTGGCGAGAG TTGCGTTTCA GAACCCGGAG 
GCCGCTCTGA GGTCTTGCTG TCATTCAGCT ATTTTCAACA AAGGTGCCTT CTCTCTCATG 
GCGAAGATCC TGCAGCAGCT GCCTGGACTC AGGGGCGAAG AGGAAAGTAT AGATGAAGGA 
AATGATGCTC TGAGTGGTGG TAGTCTGCTC TGCAGGTGTC TACAGGAGAT CATCAACACC 
AAAGCACTGT CGGCCAATGA AAAAGAGCAG TTCCTCAAGT ACGTGGGTCT GCTGATGAGG 
CCTGTCATGA CAGGCGAGGT AGAAGGAAGA AGGCGAAGCT TTCTGCCACC TCAGGAGGTC 
GTGAACATCT TTGTCCTGCC GAATCTCTCC ACCACGGGTC AAAGCTCCTT CGACATAGAG 
CTGAGTCTGC AGCTCCTCCA CACTGCTCTG TCTGTGGACG TCCAGGAACC AGACGGGTCG 
CCTCACTGGG TGTTGGACTG CTCTCCCTTT CCCCTCCTCT ATGTCCTGGC TCAGTTCCAC 
AGCCAGACTC TCAGTTGTTG GGAGCAGCCA CCAGATGGTG CTGTCTATCA CTGGTCAATG 
GACCTGAAGG AGCTGCTGGT GTCTGTGCTG ACCACCCTGG GGAACGTGGT GGGTGCAGAG 
GTGGCGGCGG TCCCCAGCAG CTGGTCCAGA GCTCTGTTCT GGCTTTACAA GAAAATGGAG 
GATCTGGACT GGACCGTTCG TTTCCACCTG AAGGCTGTTT GGGGGGAACA CTTTAAAAAC 
GAGGTTCCCT CGTCCCTGCT GGCTGTGTGT GATCTACCTG AGCAGGAGTG GTCCAGTTTG 
GACCTGCCTC AGTATGGTCA GGGTACAGGT CTTCTGGCCT GGATGGAGTG TTGTTCCATA 
TCCGACTCCC TGCAGTCCAC CATGCTGTCC CGTCTCTCTC TGGATCAGCG TCAGCTCGAC 
CACGTCAGTA TGTTCAGCAA AGGCCTGCTG GTGGCTCTGG CCCAGATCCT GCCCTGGTGC 
TCCATCTCCC AGTGGACCAG GCTGCTGAGA GCCCTGAGGG AGCTGATCAC CTCAGGCCGC 
CTCCATGTGC CCTTCTCACT CGAGTATGTG GACTACCTGC CCCTGCTGGA TCTAAGGAGG 
TTTTCATGTG AGCTCCGCCT GTCTGTCCTC CTGCTTCGAG TCCTTCAGCT GCTCTGCGGG 
TCCAGCTGCT CCGACTGGCT GTCTGCGGAC GGCTGGGCAC ACGTCGGCAG GTTATATTCC 
CACGCTGTGA GGGAGATGAT AAACTCAGTA AGAGACAAGC TGCCTCTTCC TTCATCCGGT 
GCTTTAACGT CTCCTAAAAC ACCAGCGTCT AAAGACTCTG ACCCATCGTG CACTTCCATC 
ACATCTCCTA AAATGTCCAA AGACTCTCAA AAAGACCTGA ATCAAGCAGA CGGCTCACAG 
GTGGAGGAGG AGGTGGGGAC AGTTCCCAGC CAGGAAGTCC TTTTTGTGCT CAGCCAGCTC 
TTTTGCCATG TTCAGCACAT TCAGGTGATG ATGCCAGGAG GTCAGTGTGA ACCACTCTTC 
CTGTCCAGTC TGGAGATCCT CAGCCACTAC GAGGCCATCA TGGCCGCTTT CCCCGACAGC 
AGCAGCCCGC TGGAGAGCGA CAACACCCGG CACTTCTTCT CCACCATCAC AGACAACCTG 
CAGAGCCAGG AGATGAAGTC TGTGCTGCAG CAGAAGATTG CTCAGCTCGT GTCAAAGGCA 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010731553.3
CDS	206..3571
Translation	MMDRDSAVLQGAFLLADQLCLPSSLSSLQKADWSRVGRPVLEALGEICRLGAHPTTTTISWTKKVICVVWLKLLCRETGEDLETGWRENPFFPLQNGLPDVNSAVLLEVVKSTAAAQTFAHFLLCLPQSQICAELERLVQHVRSSSTAEDDVRLLLEVWWELWKGRDEQKAGEGESIEAMFARQYTRLQSSSVPPQAAKRLKLDPSELKASSPTSDVLHILLHALKDIKDHITTTELCLQALSVSLDALYTSCLIDQAVALPPKEKIRVLSVIVSIRERNNEKLSPELIQEAVRDLHASHTPSQFRYSRMKLGEALKNITELARFWQSSELLKVCDSTNPSYSAFRLEQSVRRVLAALQKVPEAMTETDDQEAEKDTLRGLLESLALPVTESTPEVSASVTAIIISHRLDDYQNFAALFASEESWAACEQHWMDCLEKNQAAFQQCDTLIRLTSTLMRKLQSESANVSHCKKLMKVITDIFSALSLKDKNTALAAMLRLSIRGFFGRCVPSAVTEGFEQELSMAFNCIIQGRGGASAAALQGNLNTAASLVARVAFQNPEAALRSCCHSAIFNKGAFSLMAKILQQLPGLRGEEESIDEGNDALSGGSLLCRCLQEIINTKALSANEKEQFLKYVGLLMRPVMTGEVEGRRRSFLPPQEVVNIFVLPNLSTTGQSSFDIELSLQLLHTALSVDVQEPDGSPHWVLDCSPFPLLYVLAQFHSQTLSCWEQPPDGAVYHWSMDLKELLVSVLTTLGNVVGAEVAAVPSSWSRALFWLYKKMEDLDWTVRFHLKAVWGEHFKNEVPSSLLAVCDLPEQEWSSLDLPQYGQGTGLLAWMECCSISDSLQSTMLSRLSLDQRQLDHVSMFSKGLLVALAQILPWCSISQWTRLLRALRELITSGRLHVPFSLEYVDYLPLLDLRRFSCELRLSVLLLRVLQLLCGSSCSDWLSADGWAHVGRLYSHAVREMINSVRDKLPLPSSGALTSPKTPASKDSDPSCTSITSPKMSKDSQKDLNQADGSQVEEEVGTVPSQEVLFVLSQLFCHVQHIQVMMPGGQCEPLFLSSLEILSHYEAIMAAFPDSSSPLESDNTRHFFSTITDNLQSQEMKSVLQQKIAQLVSKAA

Target ORF information:

RefSeq Version	XM_010733251.3
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea gem nuclear organelle associated protein 4 (gemin4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010733251.3

ORF Insert Sequence:

ATGATGGACA GAGACTCGGC CGTCCTTCAG GGGGCTTTCC TCCTGGCTGA TCAGCTGTGT 
CTCCCCTCCT CCCTGTCCTC CCTTCAGAAA GCTGACTGGA GCAGGGTGGG ACGTCCAGTC 
CTGGAGGCAC TTGGAGAAAT CTGTAGACTC GGTGCACACC CCACGACGAC AACTATCAGC 
TGGACGAAGA AAGTCATTTG CGTTGTGTGG CTGAAGCTGC TGTGCAGGGA GACTGGAGAG 
GACCTGGAGA CGGGCTGGAG GGAGAACCCC TTTTTCCCCC TCCAGAACGG CCTCCCTGAC 
GTCAACTCCG CCGTCCTGCT GGAAGTGGTG AAGTCGACGG CGGCCGCTCA GACGTTTGCC 
CATTTCCTCT TGTGTCTTCC TCAGTCTCAG ATCTGTGCCG AGCTCGAGAG GCTGGTGCAG 
CACGTGAGGA GCAGCTCCAC CGCAGAGGAC GATGTCCGGC TTCTCCTGGA GGTGTGGTGG 
GAGCTGTGGA AAGGCAGAGA TGAGCAGAAA GCCGGAGAAG GGGAGAGCAT AGAGGCGATG 
TTTGCTCGAC AATACACTCG TCTCCAGTCT TCCAGTGTCC CCCCACAGGC TGCCAAGAGG 
TTAAAACTGG ACCCATCAGA ACTGAAGGCA TCGTCTCCAA CCTCAGATGT CCTGCACATT 
CTGCTTCATG CACTGAAAGA CATTAAAGAT CACATAACTA CAACAGAGCT TTGTCTGCAG 
GCCCTCTCGG TTTCTCTTGA TGCTCTTTAC ACATCTTGCC TGATAGACCA GGCAGTCGCA 
CTTCCACCCA AAGAGAAGAT CCGCGTCCTG TCTGTAATTG TCAGTATCAG AGAAAGGAAC 
AACGAGAAAC TGAGTCCTGA ACTTATTCAG GAGGCCGTGA GAGACCTGCA CGCTTCTCAC 
ACACCATCTC AGTTTCGATA CAGCAGGATG AAGCTTGGCG AAGCCTTGAA AAATATAACG 
GAACTGGCTC GGTTTTGGCA AAGCAGCGAG CTCCTCAAAG TGTGTGACAG CACTAATCCG 
AGCTACTCAG CATTCAGACT GGAACAGAGT GTCCGTAGAG TCCTTGCAGC TCTACAGAAA 
GTGCCTGAAG CTATGACTGA AACAGACGAT CAAGAGGCTG AGAAGGACAC GCTGAGAGGT 
TTGCTAGAGT CACTGGCTCT CCCTGTCACA GAGAGCACCC CAGAGGTGAG CGCGAGTGTC 
ACCGCGATCA TCATCAGCCA CCGCCTGGAC GACTATCAGA ACTTTGCGGC GTTATTTGCC 
AGCGAGGAAA GCTGGGCTGC CTGCGAACAG CACTGGATGG ACTGCCTGGA AAAGAACCAA 
GCAGCCTTCC AGCAGTGCGA CACGCTCATC AGACTGACCT CCACCCTCAT GCGTAAACTC 
CAGAGCGAGA GCGCCAACGT GAGCCACTGC AAGAAGCTGA TGAAAGTCAT CACAGACATT 
TTCTCTGCAC TTTCTTTAAA AGACAAGAAC ACAGCGTTGG CTGCTATGTT AAGGCTCTCC 
ATAAGGGGGT TCTTCGGACG CTGCGTCCCC TCTGCTGTGA CTGAAGGGTT CGAACAGGAG 
CTCAGCATGG CCTTCAACTG CATCATCCAA GGACGGGGTG GAGCGTCAGC AGCGGCGCTG 
CAGGGCAATC TGAACACGGC GGCTTCTTTG GTGGCGAGAG TTGCGTTTCA GAACCCGGAG 
GCCGCTCTGA GGTCTTGCTG TCATTCAGCT ATTTTCAACA AAGGTGCCTT CTCTCTCATG 
GCGAAGATCC TGCAGCAGCT GCCTGGACTC AGGGGCGAAG AGGAAAGTAT AGATGAAGGA 
AATGATGCTC TGAGTGGTGG TAGTCTGCTC TGCAGGTGTC TACAGGAGAT CATCAACACC 
AAAGCACTGT CGGCCAATGA AAAAGAGCAG TTCCTCAAGT ACGTGGGTCT GCTGATGAGG 
CCTGTCATGA CAGGCGAGGT AGAAGGAAGA AGGCGAAGCT TTCTGCCACC TCAGGAGGTC 
GTGAACATCT TTGTCCTGCC GAATCTCTCC ACCACGGGTC AAAGCTCCTT CGACATAGAG 
CTGAGTCTGC AGCTCCTCCA CACTGCTCTG TCTGTGGACG TCCAGGAACC AGACGGGTCG 
CCTCACTGGG TGTTGGACTG CTCTCCCTTT CCCCTCCTCT ATGTCCTGGC TCAGTTCCAC 
AGCCAGACTC TCAGTTGTTG GGAGCAGCCA CCAGATGGTG CTGTCTATCA CTGGTCAATG 
GACCTGAAGG AGCTGCTGGT GTCTGTGCTG ACCACCCTGG GGAACGTGGT GGGTGCAGAG 
GTGGCGGCGG TCCCCAGCAG CTGGTCCAGA GCTCTGTTCT GGCTTTACAA GAAAATGGAG 
GATCTGGACT GGACCGTTCG TTTCCACCTG AAGGCTGTTT GGGGGGAACA CTTTAAAAAC 
GAGGTTCCCT CGTCCCTGCT GGCTGTGTGT GATCTACCTG AGCAGGAGTG GTCCAGTTTG 
GACCTGCCTC AGTATGGTCA GGGTACAGGT CTTCTGGCCT GGATGGAGTG TTGTTCCATA 
TCCGACTCCC TGCAGTCCAC CATGCTGTCC CGTCTCTCTC TGGATCAGCG TCAGCTCGAC 
CACGTCAGTA TGTTCAGCAA AGGCCTGCTG GTGGCTCTGG CCCAGATCCT GCCCTGGTGC 
TCCATCTCCC AGTGGACCAG GCTGCTGAGA GCCCTGAGGG AGCTGATCAC CTCAGGCCGC 
CTCCATGTGC CCTTCTCACT CGAGTATGTG GACTACCTGC CCCTGCTGGA TCTAAGGAGG 
TTTTCATGTG AGCTCCGCCT GTCTGTCCTC CTGCTTCGAG TCCTTCAGCT GCTCTGCGGG 
TCCAGCTGCT CCGACTGGCT GTCTGCGGAC GGCTGGGCAC ACGTCGGCAG GTTATATTCC 
CACGCTGTGA GGGAGATGAT AAACTCAGTA AGAGACAAGC TGCCTCTTCC TTCATCCGGT 
GCTTTAACGT CTCCTAAAAC ACCAGCGTCT AAAGACTCTG ACCCATCGTG CACTTCCATC 
ACATCTCCTA AAATGTCCAA AGACTCTCAA AAAGACCTGA ATCAAGCAGA CGGCTCACAG 
GTGGAGGAGG AGGTGGGGAC AGTTCCCAGC CAGGAAGTCC TTTTTGTGCT CAGCCAGCTC 
TTTTGCCATG TTCAGCACAT TCAGGTGATG ATGCCAGGAG GTCAGTGTGA ACCACTCTTC 
CTGTCCAGTC TGGAGATCCT CAGCCACTAC GAGGCCATCA TGGCCGCTTT CCCCGACAGC 
AGCAGCCCGC TGGAGAGCGA CAACACCCGG CACTTCTTCT CCACCATCAC AGACAACCTG 
CAGAGCCAGG AGATGAAGTC TGTGCTGCAG CAGAAGATTG CTCAGCTCGT GTCAAAGGCA 
GCCTGA