DDX46 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX46
Entrez Gene ID	9879
Full Name	DEAD-box helicase 46
Synonyms	PRPF5,Prp5
General protein information	Preferred Names DEAD-box helicase 46 Names probable ATP-dependent RNA helicase DDX46 DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 DEAD box protein 46 PRP5 homolog Prp5-like DEAD-box protein RNA helicase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.1
Summary	This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the 17S U2 snRNP complex; it plays an important role in pre-mRNA splicing. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2014].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011543758.2	XP_011542060.1	probable ATP-dependent RNA helicase DDX46 isoform X1
NM_001300860.2	NP_001287789.1	probable ATP-dependent RNA helicase DDX46 isoform 1
NM_014829.3	NP_055644.2	probable ATP-dependent RNA helicase DDX46 isoform 2
XM_011543758.1	XP_011542060.1	probable ATP-dependent RNA helicase DDX46 isoform X1
NM_001300860.1	NP_001287789.1	probable ATP-dependent RNA helicase DDX46 isoform 1
NM_014829.4	NP_055644.2	probable ATP-dependent RNA helicase DDX46 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX46	NP_055644.2
	DDX46	XP_002804561.1
Mus musculus (house mouse)	Ddx46	NP_001268984.1
Neurospora crassa	NCU02696	XP_965469.1
Canis lupus familiaris (dog)	DDX46	XP_531911.2
Rattus norvegicus (Norway rat)	Ddx46	NP_620798.1
Drosophila melanogaster (fruit fly)	CG6227	NP_573020.2
Oryza sativa (rice)	Os08g0159900	NP_001061045.1
Xenopus tropicalis (tropical clawed frog)	ddx46	XP_004912842.1
Pan troglodytes (chimpanzee)	DDX46	XP_517939.3
Danio rerio (zebrafish)	ddx46	NP_001019988.1
Magnaporthe oryzae (rice blast fungus)	MGG_15532	XP_003713486.1
Arabidopsis thaliana (thale cress)	AT3G09620	NP_187573.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010656	XP_311375.4
Caenorhabditis elegans (roundworm)	CELE_F53H1.1	NP_001033411.1
Arabidopsis thaliana (thale cress)	AT1G20920	NP_173516.1
Gallus gallus (chicken)	DDX46	XP_004945052.1
Schizosaccharomyces pombe (fission yeast)	prp11	NP_587856.1

Gene Ontology
Bibliography

Related articles in PubMed

Knockdown of DDX46 Inhibits the Invasion and Tumorigenesis in Osteosarcoma Cells.
Jiang F, Zhang D, Li G, Wang X
Oncology research25(3)417-425(2017 Mar)

Knockdown of DDX46 inhibits proliferation and induces apoptosis in esophageal squamous cell carcinoma cells.
Li B, Li YM, He WT, Chen H, Zhu HW, Liu T, Zhang JH, Song TN, Zhou YL
Oncology reports36(1)223-30(2016 Jul)

Lentiviral DDX46 knockdown inhibits growth and induces apoptosis in human colorectal cancer cells.
Li M, Ma Y, Huang P, Du A, Yang X, Zhang S, Xing C, Liu F, Cao J
Gene560(2)237-44(2015 Apr)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Changed profile of splicing regulator genes expression in response to exercise.
Tonevitsky EA, Trushkin EV, Shkurnikov MU, Akimov EB, Sakharov DA
Bulletin of experimental biology and medicine147(6)733-6(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of DDX46 inhibited osteosarcoma cell proliferation.
Title: Knockdown of DDX46 Inhibits the Invasion and Tumorigenesis in Osteosarcoma Cells.

DDX46 is critical for ESCC cells proliferation.
Title: Knockdown of DDX46 inhibits proliferation and induces apoptosis in esophageal squamous cell carcinoma cells.

DDX46 is critical for colorectal cancer (CRC) cell proliferation and is a potential therapeutic target for CRC treatment.
Title: Lentiviral DDX46 knockdown inhibits growth and induces apoptosis in human colorectal cancer cells.

Short-term exercise resulted in a significant increase of mRNA expression of genes encoding proteins involved in the formation of precatalytic splisosome: DDX46.
Title: Changed profile of splicing regulator genes expression in response to exercise.

Prp5 forms a bridge between U1 and U2 Small Nuclear Ribonucleoproteins at the time of pre-spliceosome formation
Title: Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA.

The following DDX46 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX46 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29752	NM_014829.4 Latest version!	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu63440	XM_011543758.2 Latest version!	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu39790	NM_001300860.2 Latest version!	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu63440	XM_011543758.1	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu39790	NM_001300860.1	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu29752	NM_014829.3	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX46 ( NM_014829.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055644.2 Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu29752
Clone ID Related Accession (Same CDS sequence)	NM_014829.3 , NM_014829.4
Accession Version	NM_014829.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX46 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF244218.1, BC012304.2, AC010301.7 and BU608108.1. On Jul 15, 2014 this sequence version replaced NM_014829.2. Transcript Variant: This variant (2) has an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.19111.1, SRR1660805.62513.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCCCAGGTCA TGCGCATCGT GGATAATGTT CGTCCTGATC GACAGACGGT TATGTTTTCA 
GCTACTTTCC CCAGAGCTAT GGAGGCTTTG GCTCGCAGGA TCCTCAGTAA ACCTATTGAA 
GTACAAGTTG GAGGCAGGAG TGTGGTTTGC TCAGATGTGG AGCAACAAGT GATTGTGATT 
GAAGAAGAAA AGAAATTCTT GAAGTTACTT GAGCTTCTAG GCCATTATCA AGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CATGCTGATG GTCTTCTTAA GGATTTAATG 
AGAGCATCTT ATCCTTGCAT GTCTCTTCAT GGAGGCATTG ATCAATATGA CAGAGATAGC 
ATCATAAATG ACTTTAAGAA TGGGACCTGC AAACTTCTTG TGGCTACCTC TGTTGCTGCC 
CGAGGTCTAG ATGTGAAACA TCTGATTCTT GTAGTAAATT ATAGCTGCCC CAACCATTAT 
GAGGATTATG TACACAGAGC AGGGCGGACT GGAAGAGCAG GAAACAAGGG TTATGCTTAT 
ACTTTTATCA CAGAGGATCA AGCTCGCTAT GCTGGTGACA TAATTAAAGC TCTTGAATTG 
TCAGGGACTG CAGTACCTCC TGATTTAGAG AAACTGTGGA GTGATTTCAA AGATCAGCAG 
AAAGCTGAGG GGAAAATAAT TAAAAAGAGT AGTGGGTTCT CTGGTAAGGG ATTCAAGTTT 
GATGAAACAG AACAAGCTTT GGCTAATGAG AGGAAGAAGT TACAAAAAGC AGCTCTTGGT 
CTACAAGATT CAGATGATGA GGATGCTGCA GTTGATATTG ATGAGCAAAT TGAAAGCATG 
TTTAATTCAA AGAAGAGAGT AAAGGATATG GCTGCTCCTG GAACATCAAG TGTTCCTGCT 
CCAACTGCAG GAAATGCTGA GAAATTAGAA ATTGCTAAGA GATTGGCTCT TAGAATCAAT 
GCCCAGAAGA ATTTGGGCAT CGAGTCTCAG GATGTGATGC AGCAGGCCAC CAATGCAATT 
CTTAGGGGTG GCACCATTCT GGCTCCCACT GTTTCTGCAA AAACCATTGC AGAACAACTT 
GCTGAAAAGA TCAATGCCAA GCTCAATTAT GTGCCGTTAG AGAAACAAGA AGAAGAGAGA 
CAGGATGGTG GACAGAATGA ATCTTTTAAG AGATATGAAG AAGAATTAGA GATCAATGAC 
TTCCCACAGA CTGCTAGGTG GAAAGTTACC TCTAAGGAAG CTCTGCAGAG AATCAGTGAA 
TACTCTGAAG CCGCAATTAC AATCAGAGGA ACCTACTTCC CTCCTGGCAA AGAACCCAAG 
GAAGGCGAGC GGAAGATTTA CTTGGCAATT GAAAGTGCCA ATGAACTGGC TGTGCAGAAA 
GCAAAGGCAG AAATCACCAG GCTCATAAAA GAAGAGCTGA TCCGGCTGCA AAATTCATAC 
CAACCAACAA ATAAAGGAAG ATACAAAGTC TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055644.2
CDS	206..3301
Translation	MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL

Target ORF information:

RefSeq Version	NM_014829.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014829.3

ORF Insert Sequence:

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCCCAGGTCA TGCGCATCGT GGATAATGTT CGTCCTGATC GACAGACGGT TATGTTTTCA 
GCTACTTTCC CCAGAGCTAT GGAGGCTTTG GCTCGCAGGA TCCTCAGTAA ACCTATTGAA 
GTACAAGTTG GAGGCAGGAG TGTGGTTTGC TCAGATGTGG AGCAACAAGT GATTGTGATT 
GAAGAAGAAA AGAAATTCTT GAAGTTACTT GAGCTTCTAG GCCATTATCA AGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CATGCTGATG GTCTTCTTAA GGATTTAATG 
AGAGCATCTT ATCCTTGCAT GTCTCTTCAT GGAGGCATTG ATCAATATGA CAGAGATAGC 
ATCATAAATG ACTTTAAGAA TGGGACCTGC AAACTTCTTG TGGCTACCTC TGTTGCTGCC 
CGAGGTCTAG ATGTGAAACA TCTGATTCTT GTAGTAAATT ATAGCTGCCC CAACCATTAT 
GAGGATTATG TACACAGAGC AGGGCGGACT GGAAGAGCAG GAAACAAGGG TTATGCTTAT 
ACTTTTATCA CAGAGGATCA AGCTCGCTAT GCTGGTGACA TAATTAAAGC TCTTGAATTG 
TCAGGGACTG CAGTACCTCC TGATTTAGAG AAACTGTGGA GTGATTTCAA AGATCAGCAG 
AAAGCTGAGG GGAAAATAAT TAAAAAGAGT AGTGGGTTCT CTGGTAAGGG ATTCAAGTTT 
GATGAAACAG AACAAGCTTT GGCTAATGAG AGGAAGAAGT TACAAAAAGC AGCTCTTGGT 
CTACAAGATT CAGATGATGA GGATGCTGCA GTTGATATTG ATGAGCAAAT TGAAAGCATG 
TTTAATTCAA AGAAGAGAGT AAAGGATATG GCTGCTCCTG GAACATCAAG TGTTCCTGCT 
CCAACTGCAG GAAATGCTGA GAAATTAGAA ATTGCTAAGA GATTGGCTCT TAGAATCAAT 
GCCCAGAAGA ATTTGGGCAT CGAGTCTCAG GATGTGATGC AGCAGGCCAC CAATGCAATT 
CTTAGGGGTG GCACCATTCT GGCTCCCACT GTTTCTGCAA AAACCATTGC AGAACAACTT 
GCTGAAAAGA TCAATGCCAA GCTCAATTAT GTGCCGTTAG AGAAACAAGA AGAAGAGAGA 
CAGGATGGTG GACAGAATGA ATCTTTTAAG AGATATGAAG AAGAATTAGA GATCAATGAC 
TTCCCACAGA CTGCTAGGTG GAAAGTTACC TCTAAGGAAG CTCTGCAGAG AATCAGTGAA 
TACTCTGAAG CCGCAATTAC AATCAGAGGA ACCTACTTCC CTCCTGGCAA AGAACCCAAG 
GAAGGCGAGC GGAAGATTTA CTTGGCAATT GAAAGTGCCA ATGAACTGGC TGTGCAGAAA 
GCAAAGGCAG AAATCACCAG GCTCATAAAA GAAGAGCTGA TCCGGCTGCA AAATTCATAC 
CAACCAACAA ATAAAGGAAG ATACAAAGTC TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX46 ( NM_014829.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055644.2 Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu29752
Clone ID Related Accession (Same CDS sequence)	NM_014829.3 , NM_014829.4
Accession Version	NM_014829.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX46 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF244218.1, BC012304.2, AC010301.7 and BU608108.1. On May 31, 2019 this sequence version replaced NM_014829.3. Transcript Variant: This variant (2) has an alternate splice site in the coding region but maintains the reading frame, compared to variant 1. The resulting isoform (2) lacks an internal aa, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.62513.1, SRR1803616.19111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCCCAGGTCA TGCGCATCGT GGATAATGTT CGTCCTGATC GACAGACGGT TATGTTTTCA 
GCTACTTTCC CCAGAGCTAT GGAGGCTTTG GCTCGCAGGA TCCTCAGTAA ACCTATTGAA 
GTACAAGTTG GAGGCAGGAG TGTGGTTTGC TCAGATGTGG AGCAACAAGT GATTGTGATT 
GAAGAAGAAA AGAAATTCTT GAAGTTACTT GAGCTTCTAG GCCATTATCA AGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CATGCTGATG GTCTTCTTAA GGATTTAATG 
AGAGCATCTT ATCCTTGCAT GTCTCTTCAT GGAGGCATTG ATCAATATGA CAGAGATAGC 
ATCATAAATG ACTTTAAGAA TGGGACCTGC AAACTTCTTG TGGCTACCTC TGTTGCTGCC 
CGAGGTCTAG ATGTGAAACA TCTGATTCTT GTAGTAAATT ATAGCTGCCC CAACCATTAT 
GAGGATTATG TACACAGAGC AGGGCGGACT GGAAGAGCAG GAAACAAGGG TTATGCTTAT 
ACTTTTATCA CAGAGGATCA AGCTCGCTAT GCTGGTGACA TAATTAAAGC TCTTGAATTG 
TCAGGGACTG CAGTACCTCC TGATTTAGAG AAACTGTGGA GTGATTTCAA AGATCAGCAG 
AAAGCTGAGG GGAAAATAAT TAAAAAGAGT AGTGGGTTCT CTGGTAAGGG ATTCAAGTTT 
GATGAAACAG AACAAGCTTT GGCTAATGAG AGGAAGAAGT TACAAAAAGC AGCTCTTGGT 
CTACAAGATT CAGATGATGA GGATGCTGCA GTTGATATTG ATGAGCAAAT TGAAAGCATG 
TTTAATTCAA AGAAGAGAGT AAAGGATATG GCTGCTCCTG GAACATCAAG TGTTCCTGCT 
CCAACTGCAG GAAATGCTGA GAAATTAGAA ATTGCTAAGA GATTGGCTCT TAGAATCAAT 
GCCCAGAAGA ATTTGGGCAT CGAGTCTCAG GATGTGATGC AGCAGGCCAC CAATGCAATT 
CTTAGGGGTG GCACCATTCT GGCTCCCACT GTTTCTGCAA AAACCATTGC AGAACAACTT 
GCTGAAAAGA TCAATGCCAA GCTCAATTAT GTGCCGTTAG AGAAACAAGA AGAAGAGAGA 
CAGGATGGTG GACAGAATGA ATCTTTTAAG AGATATGAAG AAGAATTAGA GATCAATGAC 
TTCCCACAGA CTGCTAGGTG GAAAGTTACC TCTAAGGAAG CTCTGCAGAG AATCAGTGAA 
TACTCTGAAG CCGCAATTAC AATCAGAGGA ACCTACTTCC CTCCTGGCAA AGAACCCAAG 
GAAGGCGAGC GGAAGATTTA CTTGGCAATT GAAAGTGCCA ATGAACTGGC TGTGCAGAAA 
GCAAAGGCAG AAATCACCAG GCTCATAAAA GAAGAGCTGA TCCGGCTGCA AAATTCATAC 
CAACCAACAA ATAAAGGAAG ATACAAAGTC TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055644.2
CDS	161..3256
Translation	MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL

Target ORF information:

RefSeq Version	NM_014829.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014829.4

ORF Insert Sequence:

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCCCAGGTCA TGCGCATCGT GGATAATGTT CGTCCTGATC GACAGACGGT TATGTTTTCA 
GCTACTTTCC CCAGAGCTAT GGAGGCTTTG GCTCGCAGGA TCCTCAGTAA ACCTATTGAA 
GTACAAGTTG GAGGCAGGAG TGTGGTTTGC TCAGATGTGG AGCAACAAGT GATTGTGATT 
GAAGAAGAAA AGAAATTCTT GAAGTTACTT GAGCTTCTAG GCCATTATCA AGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CATGCTGATG GTCTTCTTAA GGATTTAATG 
AGAGCATCTT ATCCTTGCAT GTCTCTTCAT GGAGGCATTG ATCAATATGA CAGAGATAGC 
ATCATAAATG ACTTTAAGAA TGGGACCTGC AAACTTCTTG TGGCTACCTC TGTTGCTGCC 
CGAGGTCTAG ATGTGAAACA TCTGATTCTT GTAGTAAATT ATAGCTGCCC CAACCATTAT 
GAGGATTATG TACACAGAGC AGGGCGGACT GGAAGAGCAG GAAACAAGGG TTATGCTTAT 
ACTTTTATCA CAGAGGATCA AGCTCGCTAT GCTGGTGACA TAATTAAAGC TCTTGAATTG 
TCAGGGACTG CAGTACCTCC TGATTTAGAG AAACTGTGGA GTGATTTCAA AGATCAGCAG 
AAAGCTGAGG GGAAAATAAT TAAAAAGAGT AGTGGGTTCT CTGGTAAGGG ATTCAAGTTT 
GATGAAACAG AACAAGCTTT GGCTAATGAG AGGAAGAAGT TACAAAAAGC AGCTCTTGGT 
CTACAAGATT CAGATGATGA GGATGCTGCA GTTGATATTG ATGAGCAAAT TGAAAGCATG 
TTTAATTCAA AGAAGAGAGT AAAGGATATG GCTGCTCCTG GAACATCAAG TGTTCCTGCT 
CCAACTGCAG GAAATGCTGA GAAATTAGAA ATTGCTAAGA GATTGGCTCT TAGAATCAAT 
GCCCAGAAGA ATTTGGGCAT CGAGTCTCAG GATGTGATGC AGCAGGCCAC CAATGCAATT 
CTTAGGGGTG GCACCATTCT GGCTCCCACT GTTTCTGCAA AAACCATTGC AGAACAACTT 
GCTGAAAAGA TCAATGCCAA GCTCAATTAT GTGCCGTTAG AGAAACAAGA AGAAGAGAGA 
CAGGATGGTG GACAGAATGA ATCTTTTAAG AGATATGAAG AAGAATTAGA GATCAATGAC 
TTCCCACAGA CTGCTAGGTG GAAAGTTACC TCTAAGGAAG CTCTGCAGAG AATCAGTGAA 
TACTCTGAAG CCGCAATTAC AATCAGAGGA ACCTACTTCC CTCCTGGCAA AGAACCCAAG 
GAAGGCGAGC GGAAGATTTA CTTGGCAATT GAAAGTGCCA ATGAACTGGC TGTGCAGAAA 
GCAAAGGCAG AAATCACCAG GCTCATAAAA GAAGAGCTGA TCCGGCTGCA AAATTCATAC 
CAACCAACAA ATAAAGGAAG ATACAAAGTC TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX46 ( XM_011543758.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542060.1 Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant X2, mRNA.

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CloneID	OHu63440
Clone ID Related Accession (Same CDS sequence)	XM_011543758.2 , XM_011543758.1
Accession Version	XM_011543758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX46 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543758.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGCCCTTC AAGATTTATA 
GAACTGGGCT TCCGAGACTT AAACCATCAA AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542060.1
CDS	162..1757
Translation	MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGPSRFIELGFRDLNHQR

Target ORF information:

RefSeq Version	XM_011543758.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543758.2

ORF Insert Sequence:

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGCCCTTC AAGATTTATA 
GAACTGGGCT TCCGAGACTT AAACCATCAA AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX46 ( XM_011543758.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542060.1 Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant X2, mRNA.

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CloneID	OHu63440
Clone ID Related Accession (Same CDS sequence)	XM_011543758.2 , XM_011543758.1
Accession Version	XM_011543758.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX46 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGCCCTTC AAGATTTATA 
GAACTGGGCT TCCGAGACTT AAACCATCAA AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542060.1
CDS	169..1764
Translation	MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGPSRFIELGFRDLNHQR

Target ORF information:

RefSeq Version	XM_011543758.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543758.1

ORF Insert Sequence:

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGCCCTTC AAGATTTATA 
GAACTGGGCT TCCGAGACTT AAACCATCAA AGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX46 ( NM_001300860.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287789.1 Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu39790
Clone ID Related Accession (Same CDS sequence)	NM_001300860.2 , NM_001300860.1
Accession Version	NM_001300860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX46 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF244218.1, BK000565.1, AC010301.7 and BU608108.1. On Nov 22, 2018 this sequence version replaced NM_001300860.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.182204.1, SRR1803614.104240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000452510.7/ ENSP00000416534.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCCCAGGTCA TGCGCATCGT GGATAATGTT CGTCCTGATC GACAGACGGT TATGTTTTCA 
GCTACTTTCC CCAGAGCTAT GGAGGCTTTG GCTCGCAGGA TCCTCAGTAA ACCTATTGAA 
GTACAAGTTG GAGGCAGGAG TGTGGTTTGC TCAGATGTGG AGCAACAAGT GATTGTGATT 
GAAGAAGAAA AGAAATTCTT GAAGTTACTT GAGCTTCTAG GCCATTATCA AGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CATGCTGATG GTCTTCTTAA GGATTTAATG 
AGAGCATCTT ATCCTTGCAT GTCTCTTCAT GGAGGCATTG ATCAATATGA CAGAGATAGC 
ATCATAAATG ACTTTAAGAA TGGGACCTGC AAACTTCTTG TGGCTACCTC TGTTGCTGCC 
CGAGGTCTAG ATGTGAAACA TCTGATTCTT GTAGTAAATT ATAGCTGCCC CAACCATTAT 
GAGGATTATG TACACAGAGC AGGGCGGACT GGAAGAGCAG GAAACAAGGG TTATGCTTAT 
ACTTTTATCA CAGAGGATCA AGCTCGCTAT GCTGGTGACA TAATTAAAGC TCTTGAATTG 
TCAGGGACTG CAGTACCTCC TGATTTAGAG AAACTGTGGA GTGATTTCAA AGATCAGCAG 
AAAGCTGAGG GGAAAATAAT TAAAAAGAGT AGTGGGTTCT CTGGTAAGGG ATTCAAGTTT 
GATGAAACAG AACAAGCTTT GGCTAATGAG AGGAAGAAGT TACAAAAAGC AGCTCTTGGT 
CTACAAGATT CAGATGATGA GGATGCTGCA GTTGATATTG ATGAGCAAAT TGAAAGCATG 
TTTAATTCAA AGAAGAGAGT AAAGGATATG GCTGCTCCTG GAACATCAAG TGTTCCTGCT 
CCAACTGCAG GAAATGCTGA GAAATTAGAA ATTGCTAAGA GATTGGCTCT TAGAATCAAT 
GCCCAGAAGA ATTTGGGCAT CGAGTCTCAG GTAGATGTGA TGCAGCAGGC CACCAATGCA 
ATTCTTAGGG GTGGCACCAT TCTGGCTCCC ACTGTTTCTG CAAAAACCAT TGCAGAACAA 
CTTGCTGAAA AGATCAATGC CAAGCTCAAT TATGTGCCGT TAGAGAAACA AGAAGAAGAG 
AGACAGGATG GTGGACAGAA TGAATCTTTT AAGAGATATG AAGAAGAATT AGAGATCAAT 
GACTTCCCAC AGACTGCTAG GTGGAAAGTT ACCTCTAAGG AAGCTCTGCA GAGAATCAGT 
GAATACTCTG AAGCCGCAAT TACAATCAGA GGAACCTACT TCCCTCCTGG CAAAGAACCC 
AAGGAAGGCG AGCGGAAGAT TTACTTGGCA ATTGAAAGTG CCAATGAACT GGCTGTGCAG 
AAAGCAAAGG CAGAAATCAC CAGGCTCATA AAAGAAGAGC TGATCCGGCT GCAAAATTCA 
TACCAACCAA CAAATAAAGG AAGATACAAA GTCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287789.1
CDS	161..3259
Translation	MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQVDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL

Target ORF information:

RefSeq Version	NM_001300860.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300860.2

ORF Insert Sequence:

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCCCAGGTCA TGCGCATCGT GGATAATGTT CGTCCTGATC GACAGACGGT TATGTTTTCA 
GCTACTTTCC CCAGAGCTAT GGAGGCTTTG GCTCGCAGGA TCCTCAGTAA ACCTATTGAA 
GTACAAGTTG GAGGCAGGAG TGTGGTTTGC TCAGATGTGG AGCAACAAGT GATTGTGATT 
GAAGAAGAAA AGAAATTCTT GAAGTTACTT GAGCTTCTAG GCCATTATCA AGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CATGCTGATG GTCTTCTTAA GGATTTAATG 
AGAGCATCTT ATCCTTGCAT GTCTCTTCAT GGAGGCATTG ATCAATATGA CAGAGATAGC 
ATCATAAATG ACTTTAAGAA TGGGACCTGC AAACTTCTTG TGGCTACCTC TGTTGCTGCC 
CGAGGTCTAG ATGTGAAACA TCTGATTCTT GTAGTAAATT ATAGCTGCCC CAACCATTAT 
GAGGATTATG TACACAGAGC AGGGCGGACT GGAAGAGCAG GAAACAAGGG TTATGCTTAT 
ACTTTTATCA CAGAGGATCA AGCTCGCTAT GCTGGTGACA TAATTAAAGC TCTTGAATTG 
TCAGGGACTG CAGTACCTCC TGATTTAGAG AAACTGTGGA GTGATTTCAA AGATCAGCAG 
AAAGCTGAGG GGAAAATAAT TAAAAAGAGT AGTGGGTTCT CTGGTAAGGG ATTCAAGTTT 
GATGAAACAG AACAAGCTTT GGCTAATGAG AGGAAGAAGT TACAAAAAGC AGCTCTTGGT 
CTACAAGATT CAGATGATGA GGATGCTGCA GTTGATATTG ATGAGCAAAT TGAAAGCATG 
TTTAATTCAA AGAAGAGAGT AAAGGATATG GCTGCTCCTG GAACATCAAG TGTTCCTGCT 
CCAACTGCAG GAAATGCTGA GAAATTAGAA ATTGCTAAGA GATTGGCTCT TAGAATCAAT 
GCCCAGAAGA ATTTGGGCAT CGAGTCTCAG GTAGATGTGA TGCAGCAGGC CACCAATGCA 
ATTCTTAGGG GTGGCACCAT TCTGGCTCCC ACTGTTTCTG CAAAAACCAT TGCAGAACAA 
CTTGCTGAAA AGATCAATGC CAAGCTCAAT TATGTGCCGT TAGAGAAACA AGAAGAAGAG 
AGACAGGATG GTGGACAGAA TGAATCTTTT AAGAGATATG AAGAAGAATT AGAGATCAAT 
GACTTCCCAC AGACTGCTAG GTGGAAAGTT ACCTCTAAGG AAGCTCTGCA GAGAATCAGT 
GAATACTCTG AAGCCGCAAT TACAATCAGA GGAACCTACT TCCCTCCTGG CAAAGAACCC 
AAGGAAGGCG AGCGGAAGAT TTACTTGGCA ATTGAAAGTG CCAATGAACT GGCTGTGCAG 
AAAGCAAAGG CAGAAATCAC CAGGCTCATA AAAGAAGAGC TGATCCGGCT GCAAAATTCA 
TACCAACCAA CAAATAAAGG AAGATACAAA GTCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX46 ( NM_001300860.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287789.1 Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu39790
Clone ID Related Accession (Same CDS sequence)	NM_001300860.2 , NM_001300860.1
Accession Version	NM_001300860.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX46 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BF244218.1, BK000565.1, AC010301.7 and BU608108.1. On Jul 15, 2014 this sequence version replaced XM_005272142.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.182204.1, SRR1803614.104240.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCCCAGGTCA TGCGCATCGT GGATAATGTT CGTCCTGATC GACAGACGGT TATGTTTTCA 
GCTACTTTCC CCAGAGCTAT GGAGGCTTTG GCTCGCAGGA TCCTCAGTAA ACCTATTGAA 
GTACAAGTTG GAGGCAGGAG TGTGGTTTGC TCAGATGTGG AGCAACAAGT GATTGTGATT 
GAAGAAGAAA AGAAATTCTT GAAGTTACTT GAGCTTCTAG GCCATTATCA AGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CATGCTGATG GTCTTCTTAA GGATTTAATG 
AGAGCATCTT ATCCTTGCAT GTCTCTTCAT GGAGGCATTG ATCAATATGA CAGAGATAGC 
ATCATAAATG ACTTTAAGAA TGGGACCTGC AAACTTCTTG TGGCTACCTC TGTTGCTGCC 
CGAGGTCTAG ATGTGAAACA TCTGATTCTT GTAGTAAATT ATAGCTGCCC CAACCATTAT 
GAGGATTATG TACACAGAGC AGGGCGGACT GGAAGAGCAG GAAACAAGGG TTATGCTTAT 
ACTTTTATCA CAGAGGATCA AGCTCGCTAT GCTGGTGACA TAATTAAAGC TCTTGAATTG 
TCAGGGACTG CAGTACCTCC TGATTTAGAG AAACTGTGGA GTGATTTCAA AGATCAGCAG 
AAAGCTGAGG GGAAAATAAT TAAAAAGAGT AGTGGGTTCT CTGGTAAGGG ATTCAAGTTT 
GATGAAACAG AACAAGCTTT GGCTAATGAG AGGAAGAAGT TACAAAAAGC AGCTCTTGGT 
CTACAAGATT CAGATGATGA GGATGCTGCA GTTGATATTG ATGAGCAAAT TGAAAGCATG 
TTTAATTCAA AGAAGAGAGT AAAGGATATG GCTGCTCCTG GAACATCAAG TGTTCCTGCT 
CCAACTGCAG GAAATGCTGA GAAATTAGAA ATTGCTAAGA GATTGGCTCT TAGAATCAAT 
GCCCAGAAGA ATTTGGGCAT CGAGTCTCAG GTAGATGTGA TGCAGCAGGC CACCAATGCA 
ATTCTTAGGG GTGGCACCAT TCTGGCTCCC ACTGTTTCTG CAAAAACCAT TGCAGAACAA 
CTTGCTGAAA AGATCAATGC CAAGCTCAAT TATGTGCCGT TAGAGAAACA AGAAGAAGAG 
AGACAGGATG GTGGACAGAA TGAATCTTTT AAGAGATATG AAGAAGAATT AGAGATCAAT 
GACTTCCCAC AGACTGCTAG GTGGAAAGTT ACCTCTAAGG AAGCTCTGCA GAGAATCAGT 
GAATACTCTG AAGCCGCAAT TACAATCAGA GGAACCTACT TCCCTCCTGG CAAAGAACCC 
AAGGAAGGCG AGCGGAAGAT TTACTTGGCA ATTGAAAGTG CCAATGAACT GGCTGTGCAG 
AAAGCAAAGG CAGAAATCAC CAGGCTCATA AAAGAAGAGC TGATCCGGCT GCAAAATTCA 
TACCAACCAA CAAATAAAGG AAGATACAAA GTCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287789.1
CDS	206..3304
Translation	MGRESRHYRKRSASRGRSGSRSRSRSPSDKRSKRGDDRRSRSRDRDRRRERSRSRDKRRSRSRDRKRLRRSRSRERDRSRERRRSRSRDRRRSRSRSRGRRSRSSSPGNKSKKTENRSRSKEKTDGGESSKEKKKDKDDKEDEKEKDAGNFDQNKLEEEMRKRKERVEKWREEQRKKAMENIGELKKEIEEMKQGKKWSLEDDDDDEDDPAEAEKEGNEMEGEELDPLDAYMEEVKEEVKKFNMRSVKGGGGNEKKSGPTVTKVVTVVTTKKAVVDSDKKKGELMENDQDAMEYSSEEEEVDLQTALTGYQTKQRKLLEPVDHGKIEYEPFRKNFYVEVPELAKMSQEEVNVFRLEMEGITVKGKGCPKPIKSWVQCGISMKILNSLKKHGYEKPTPIQTQAIPAIMSGRDLIGIAKTGSGKTIAFLLPMFRHIMDQRSLEEGEGPIAVIMTPTRELALQITKECKKFSKTLGLRVVCVYGGTGISEQIAELKRGAEIIVCTPGRMIDMLAANSGRVTNLRRVTYVVLDEADRMFDMGFEPQVMRIVDNVRPDRQTVMFSATFPRAMEALARRILSKPIEVQVGGRSVVCSDVEQQVIVIEEEKKFLKLLELLGHYQESGSVIIFVDKQEHADGLLKDLMRASYPCMSLHGGIDQYDRDSIINDFKNGTCKLLVATSVAARGLDVKHLILVVNYSCPNHYEDYVHRAGRTGRAGNKGYAYTFITEDQARYAGDIIKALELSGTAVPPDLEKLWSDFKDQQKAEGKIIKKSSGFSGKGFKFDETEQALANERKKLQKAALGLQDSDDEDAAVDIDEQIESMFNSKKRVKDMAAPGTSSVPAPTAGNAEKLEIAKRLALRINAQKNLGIESQVDVMQQATNAILRGGTILAPTVSAKTIAEQLAEKINAKLNYVPLEKQEEERQDGGQNESFKRYEEELEINDFPQTARWKVTSKEALQRISEYSEAAITIRGTYFPPGKEPKEGERKIYLAIESANELAVQKAKAEITRLIKEELIRLQNSYQPTNKGRYKVL

Target ORF information:

RefSeq Version	NM_001300860.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 46 (DDX46), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300860.1

ORF Insert Sequence:

ATGGGTCGGG AGTCACGCCA CTATCGAAAA CGATCGGCAT CCCGGGGTCG CTCTGGAAGT 
CGGTCTAGAA GTCGCTCACC CTCAGACAAA AGAAGTAAAC GTGGAGATGA CAGACGGTCT 
AGAAGTAGAG ATAGAGATAG GAGGAGAGAG AGGTCTCGTA GCAGGGATAA AAGAAGATCT 
CGGTCAAGGG ACAGGAAGCG TCTGAGACGT TCCAGAAGTA GAGAGAGAGA CAGAAGCCGA 
GAGCGAAGAA GATCTCGAAG TAGAGACAGG AGACGCTCAA GGAGTAGAAG CCGGGGCCGG 
CGATCCCGAT CCTCCAGTCC TGGAAATAAA AGCAAGAAAA CTGAGAATAG ATCTAGGTCC 
AAAGAGAAAA CTGATGGTGG GGAAAGTTCT AAAGAGAAGA AAAAAGACAA AGATGACAAG 
GAGGATGAAA AAGAAAAAGA TGCTGGCAAC TTTGACCAGA ATAAGCTGGA AGAAGAAATG 
AGAAAGCGAA AAGAAAGAGT AGAAAAATGG CGAGAAGAGC AACGTAAAAA GGCTATGGAA 
AACATAGGAG AACTGAAAAA GGAAATCGAA GAGATGAAAC AAGGGAAAAA GTGGAGTTTA 
GAGGACGATG ATGATGACGA AGATGATCCT GCAGAAGCTG AAAAGGAGGG AAATGAAATG 
GAGGGTGAGG AGTTAGATCC ATTAGATGCT TACATGGAAG AAGTGAAAGA GGAAGTAAAA 
AAATTTAACA TGAGAAGTGT AAAAGGTGGT GGGGGAAATG AAAAGAAGTC TGGGCCAACG 
GTCACAAAAG TTGTCACTGT TGTGACAACC AAAAAAGCAG TTGTGGATTC TGATAAGAAG 
AAAGGTGAGC TGATGGAGAA TGACCAGGAT GCCATGGAGT ATTCTTCAGA GGAGGAAGAA 
GTTGATCTTC AGACAGCCCT TACAGGGTAT CAAACAAAAC AGCGAAAGCT TCTAGAACCA 
GTTGATCATG GAAAAATTGA GTATGAGCCA TTTAGGAAAA ACTTCTATGT TGAAGTTCCA 
GAACTAGCAA AAATGTCTCA AGAAGAGGTA AATGTGTTTC GATTGGAAAT GGAGGGCATT 
ACAGTTAAAG GAAAAGGTTG CCCCAAACCA ATTAAATCCT GGGTCCAGTG TGGAATTTCC 
ATGAAGATCT TAAATTCCCT CAAGAAGCAT GGCTATGAAA AGCCCACGCC CATCCAAACC 
CAAGCTATTC CTGCTATAAT GTCTGGACGA GATTTGATTG GCATTGCCAA AACAGGAAGT 
GGAAAGACCA TTGCTTTTCT GTTGCCCATG TTTAGACACA TCATGGATCA GAGGTCATTA 
GAGGAAGGAG AGGGGCCAAT AGCTGTCATC ATGACTCCAA CTCGAGAACT GGCTTTACAG 
ATTACTAAAG AGTGTAAGAA GTTTTCCAAG ACTTTGGGAC TTAGAGTGGT CTGTGTTTAC 
GGAGGAACAG GAATCAGTGA GCAGATTGCT GAGCTGAAAA GAGGTGCTGA AATTATTGTT 
TGCACACCTG GTCGAATGAT TGACATGTTA GCCGCTAACA GTGGTCGGGT CACAAATCTT 
CGAAGAGTGA CATATGTTGT TTTAGATGAA GCAGACAGAA TGTTTGACAT GGGTTTTGAA 
CCCCAGGTCA TGCGCATCGT GGATAATGTT CGTCCTGATC GACAGACGGT TATGTTTTCA 
GCTACTTTCC CCAGAGCTAT GGAGGCTTTG GCTCGCAGGA TCCTCAGTAA ACCTATTGAA 
GTACAAGTTG GAGGCAGGAG TGTGGTTTGC TCAGATGTGG AGCAACAAGT GATTGTGATT 
GAAGAAGAAA AGAAATTCTT GAAGTTACTT GAGCTTCTAG GCCATTATCA AGAGTCAGGA 
TCTGTCATTA TATTTGTGGA TAAGCAGGAA CATGCTGATG GTCTTCTTAA GGATTTAATG 
AGAGCATCTT ATCCTTGCAT GTCTCTTCAT GGAGGCATTG ATCAATATGA CAGAGATAGC 
ATCATAAATG ACTTTAAGAA TGGGACCTGC AAACTTCTTG TGGCTACCTC TGTTGCTGCC 
CGAGGTCTAG ATGTGAAACA TCTGATTCTT GTAGTAAATT ATAGCTGCCC CAACCATTAT 
GAGGATTATG TACACAGAGC AGGGCGGACT GGAAGAGCAG GAAACAAGGG TTATGCTTAT 
ACTTTTATCA CAGAGGATCA AGCTCGCTAT GCTGGTGACA TAATTAAAGC TCTTGAATTG 
TCAGGGACTG CAGTACCTCC TGATTTAGAG AAACTGTGGA GTGATTTCAA AGATCAGCAG 
AAAGCTGAGG GGAAAATAAT TAAAAAGAGT AGTGGGTTCT CTGGTAAGGG ATTCAAGTTT 
GATGAAACAG AACAAGCTTT GGCTAATGAG AGGAAGAAGT TACAAAAAGC AGCTCTTGGT 
CTACAAGATT CAGATGATGA GGATGCTGCA GTTGATATTG ATGAGCAAAT TGAAAGCATG 
TTTAATTCAA AGAAGAGAGT AAAGGATATG GCTGCTCCTG GAACATCAAG TGTTCCTGCT 
CCAACTGCAG GAAATGCTGA GAAATTAGAA ATTGCTAAGA GATTGGCTCT TAGAATCAAT 
GCCCAGAAGA ATTTGGGCAT CGAGTCTCAG GTAGATGTGA TGCAGCAGGC CACCAATGCA 
ATTCTTAGGG GTGGCACCAT TCTGGCTCCC ACTGTTTCTG CAAAAACCAT TGCAGAACAA 
CTTGCTGAAA AGATCAATGC CAAGCTCAAT TATGTGCCGT TAGAGAAACA AGAAGAAGAG 
AGACAGGATG GTGGACAGAA TGAATCTTTT AAGAGATATG AAGAAGAATT AGAGATCAAT 
GACTTCCCAC AGACTGCTAG GTGGAAAGTT ACCTCTAAGG AAGCTCTGCA GAGAATCAGT 
GAATACTCTG AAGCCGCAAT TACAATCAGA GGAACCTACT TCCCTCCTGG CAAAGAACCC 
AAGGAAGGCG AGCGGAAGAT TTACTTGGCA ATTGAAAGTG CCAATGAACT GGCTGTGCAG 
AAAGCAAAGG CAGAAATCAC CAGGCTCATA AAAGAAGAGC TGATCCGGCT GCAAAATTCA 
TACCAACCAA CAAATAAAGG AAGATACAAA GTCTTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knockdown of DDX46 Inhibits the Invasion and Tumorigenesis in Osteosarcoma Cells.
Oncology research25(3)417-425(2017 Mar)
Jiang F,Zhang D,Li G,Wang X

Knockdown of DDX46 inhibits proliferation and induces apoptosis in esophageal squamous cell carcinoma cells.
Oncology reports36(1)223-30(2016 Jul)
Li B,Li YM,He WT,Chen H,Zhu HW,Liu T,Zhang JH,Song TN,Zhou YL

Lentiviral DDX46 knockdown inhibits growth and induces apoptosis in human colorectal cancer cells.
Gene560(2)237-44(2015 Apr)
Li M,Ma Y,Huang P,Du A,Yang X,Zhang S,Xing C,Liu F,Cao J

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Changed profile of splicing regulator genes expression in response to exercise.
Bulletin of experimental biology and medicine147(6)733-6(2009 Jun)
Tonevitsky EA,Trushkin EV,Shkurnikov MU,Akimov EB,Sakharov DA

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DDX46 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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