EEF1E1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EEF1E1
Entrez Gene ID	9521
Full Name	eukaryotic translation elongation factor 1 epsilon 1
Synonyms	AIMP3,P18
General protein information	Preferred Names eukaryotic translation elongation factor 1 epsilon 1 Names eukaryotic translation elongation factor 1 epsilon-1 ARS-interacting multifunctional protein 3 aminoacyl tRNA synthetase complex-interacting multifunctional protein 3 multisynthase complex auxiliary component p18 p18 component of aminoacyl-tRNA synthetase complex
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p24.3
Summary	This gene encodes a multifunctional protein that localizes to both the cytoplasm and nucleus. In the cytoplasm, the encoded protein is an auxiliary component of the macromolecular aminoacyl-tRNA synthase complex. However, its mouse homolog has been shown to translocate to the nucleus in response to DNA damage, and it plays a positive role in ATM/ATR-mediated p53 activation. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring downstream MUTED (muted homolog) gene. An EEF1E1-related pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2010].
Disorder MIM:	609206

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135650.1	NP_001129122.1	eukaryotic translation elongation factor 1 epsilon-1 isoform 2
NM_004280.4	NP_004271.1	eukaryotic translation elongation factor 1 epsilon-1 isoform 1
NM_004280.5	NP_004271.1	eukaryotic translation elongation factor 1 epsilon-1 isoform 1
NM_001135650.2	NP_001129122.1	eukaryotic translation elongation factor 1 epsilon-1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	EEF1E1	NP_004271.1
Danio rerio (zebrafish)	eef1e1	XP_692806.3
Mus musculus (house mouse)	Eef1e1	NP_079656.1
Rattus norvegicus (Norway rat)	Eef1e1	NP_001099576.1
Pan troglodytes (chimpanzee)	EEF1E1	XP_001166234.1
	EEF1E1	XP_001086399.1
Canis lupus familiaris (dog)	EEF1E1	XP_535882.1
Bos taurus (cattle)	EEF1E1	NP_001035689.1
Gallus gallus (chicken)	EEF1E1	XP_418953.4
Xenopus tropicalis (tropical clawed frog)	eef1e1	NP_001107137.1

Gene Ontology
Bibliography

Related articles in PubMed

Mapping the contact surfaces in the Lamin A:AIMP3 complex by hydrogen/deuterium exchange FT-ICR mass spectrometry.
Tao Y, Fang P, Kim S, Guo M, Young NL, Marshall AG
PloS one12(8)e0181869(2017)

Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric Glutathione Transferase-homology Domains.
Cho HY, Maeng SJ, Cho HJ, Choi YS, Chung JM, Lee S, Kim HK, Kim JH, Eom CY, Kim YG, Guo M, Jung HS, Kang BS, Kim S
The Journal of biological chemistry290(49)29313-28(2015 Dec)

miR-543 and miR-590-3p regulate human mesenchymal stem cell aging via direct targeting of AIMP3/p18.
Lee S, Yu KR, Ryu YS, Oh YS, Hong IS, Kim HS, Lee JY, Kim S, Seo KW, Kang KS
Age (Dordrecht, Netherlands)36(6)9724(2014)

Identification of two novel BRCA1-partner genes in the DNA double-strand break repair pathway.
Guglielmi C, Cerri I, Evangelista M, Collavoli A, Tancredi M, Aretini P, Caligo MA
Breast cancer research and treatment141(3)515-22(2013 Oct)

Genetics of coronary artery calcification among African Americans, a meta-analysis.
Wojczynski MK, Li M, Bielak LF, Kerr KF, Reiner AP, Wong ND, Yanek LR, Qu L, White CC, Lange LA, Ferguson JF, He J, Young T, Mosley TH, Smith JA, Kral BG, Guo X, Wong Q, Ganesh SK, Heckbert SR, Griswold ME, O'Leary DH, Budoff M, Carr JJ, Taylor HA Jr, Bluemke DA, Demissie S, Hwang SJ, Paltoo DN, Polak JF, Psaty BM, Becker DM, Province MA, Post WS, O'Donnell CJ, Wilson JG, Harris TB, Kavousi M, Cupples LA, Rotter JI, Fornage M, Becker LC, Peyser PA, Borecki IB, Reilly MP
BMC medical genetics1475(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LmnA binds AIMP3 via its extreme C-terminus. Together these findings provide a structural insight for understanding the interaction between AIMP3 and LmnA in AIMP3 degradation.
Title: Mapping the contact surfaces in the Lamin A:AIMP3 complex by hydrogen/deuterium exchange FT-ICR mass spectrometry.

analysis of the heterotetrameric complex structure of the glutathione transferase (GST) domains shared among the four MSC components, methionyl-tRNA synthetase (MRS), glutaminyl-prolyl-tRNA synthetase (EPRS), AIMP2 and AIMP3
Title: Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric Glutathione Transferase-homology Domains.

AIMP3 was shown to be involved in the cellular aging of human mesenchymal stem cells.
Title: miR-543 and miR-590-3p regulate human mesenchymal stem cell aging via direct targeting of AIMP3/p18.

Identification of EEf1E1 and OBFC2B as novel BRCA1-partner genes in the DNA double-strand break repair pathway.
Title: Identification of two novel BRCA1-partner genes in the DNA double-strand break repair pathway.

AIMP3 stabilizes and protects methionyl-tRNA synthetase and eIF2gamma through the binding interactions.
Title: Contributions of aminoacyl-tRNA synthetase-interacting multifunctional protein-3 to mammalian translation initiation.

The following EEF1E1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EEF1E1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04663	NM_004280.5 Latest version!	Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu07328	NM_001135650.2 Latest version!	Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu04663	NM_004280.4	Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu07328	NM_001135650.1	Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EEF1E1 ( NM_004280.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004271.1 Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04663
Clone ID Related Accession (Same CDS sequence)	NM_004280.4 , NM_004280.5
Accession Version	NM_004280.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-08
Organism	Homo sapiens(human)
Product	eukaryotic translation elongation factor 1 epsilon-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV714140.2, BP235196.1, BC005291.1, AF054186.1, BG183264.1 and AI374688.1. On Oct 7, 2008 this sequence version replaced NM_004280.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.250310.1, SRR1163657.80401.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMN03267753 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG CCGCAGAGTT GTCGCTACTG GAGAAGTCCC TGGGACTGAG TAAGGGGAAT 
AAATACAGTG CTCAGGGCGA GCGACAGATT CCAGTTCTTC AGACAAACAA TGGTCCAAGT 
CTAACAGGAT TGACTACTAT AGCAGCTCAT CTAGTCAAGC AAGCCAACAA AGAATATTTG 
CTGGGGAGTA CTGCAGAAGA AAAAGCAATC GTTCAGCAGT GGTTAGAATA CAGGGTCACT 
CAAGTAGATG GGCACTCCAG TAAAAATGAC ATCCACACAC TGTTGAAGGA TCTTAATTCA 
TATCTTGAAG ATAAAGTCTA CCTTACAGGG TATAACTTTA CATTAGCAGA TATACTATTG 
TACTATGGAC TTCATCGCTT TATAGTTGAC CTGACAGTTC AAGAAAAGGA GAAATATCTT 
AATGTGTCTC GCTGGTTTTG TCACATTCAG CATTATCCAG GCATCAGGCA ACATCTGTCT 
AGTGTTGTCT TCATCAAGAA CAGACTATAT ACTAATTCCC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004271.1
CDS	75..599
Translation	MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNSYLEDKVYLTGYNFTLADILLYYGLHRFIVDLTVQEKEKYLNVSRWFCHIQHYPGIRQHLSSVVFIKNRLYTNSH

Target ORF information:

RefSeq Version	NM_004280.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004280.4

ORF Insert Sequence:

ATGGCGGCGG CCGCAGAGTT GTCGCTACTG GAGAAGTCCC TGGGACTGAG TAAGGGGAAT 
AAATACAGTG CTCAGGGCGA GCGACAGATT CCAGTTCTTC AGACAAACAA TGGTCCAAGT 
CTAACAGGAT TGACTACTAT AGCAGCTCAT CTAGTCAAGC AAGCCAACAA AGAATATTTG 
CTGGGGAGTA CTGCAGAAGA AAAAGCAATC GTTCAGCAGT GGTTAGAATA CAGGGTCACT 
CAAGTAGATG GGCACTCCAG TAAAAATGAC ATCCACACAC TGTTGAAGGA TCTTAATTCA 
TATCTTGAAG ATAAAGTCTA CCTTACAGGG TATAACTTTA CATTAGCAGA TATACTATTG 
TACTATGGAC TTCATCGCTT TATAGTTGAC CTGACAGTTC AAGAAAAGGA GAAATATCTT 
AATGTGTCTC GCTGGTTTTG TCACATTCAG CATTATCCAG GCATCAGGCA ACATCTGTCT 
AGTGTTGTCT TCATCAAGAA CAGACTATAT ACTAATTCCC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1E1 ( NM_004280.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004271.1 Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04663
Clone ID Related Accession (Same CDS sequence)	NM_004280.4 , NM_004280.5
Accession Version	NM_004280.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	eukaryotic translation elongation factor 1 epsilon-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005291.1, AF054186.1, BG183264.1 and AI374688.1. On Nov 22, 2018 this sequence version replaced NM_004280.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.45773.1, SRR1660805.40425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144835, SAMN03267753 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379715.10/ ENSP00000369038.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCGG CCGCAGAGTT GTCGCTACTG GAGAAGTCCC TGGGACTGAG TAAGGGGAAT 
AAATACAGTG CTCAGGGCGA GCGACAGATT CCAGTTCTTC AGACAAACAA TGGTCCAAGT 
CTAACAGGAT TGACTACTAT AGCAGCTCAT CTAGTCAAGC AAGCCAACAA AGAATATTTG 
CTGGGGAGTA CTGCAGAAGA AAAAGCAATC GTTCAGCAGT GGTTAGAATA CAGGGTCACT 
CAAGTAGATG GGCACTCCAG TAAAAATGAC ATCCACACAC TGTTGAAGGA TCTTAATTCA 
TATCTTGAAG ATAAAGTCTA CCTTACAGGG TATAACTTTA CATTAGCAGA TATACTATTG 
TACTATGGAC TTCATCGCTT TATAGTTGAC CTGACAGTTC AAGAAAAGGA GAAATATCTT 
AATGTGTCTC GCTGGTTTTG TCACATTCAG CATTATCCAG GCATCAGGCA ACATCTGTCT 
AGTGTTGTCT TCATCAAGAA CAGACTATAT ACTAATTCCC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004271.1
CDS	28..552
Translation	MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNSYLEDKVYLTGYNFTLADILLYYGLHRFIVDLTVQEKEKYLNVSRWFCHIQHYPGIRQHLSSVVFIKNRLYTNSH

Target ORF information:

RefSeq Version	NM_004280.5
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004280.5

ORF Insert Sequence:

ATGGCGGCGG CCGCAGAGTT GTCGCTACTG GAGAAGTCCC TGGGACTGAG TAAGGGGAAT 
AAATACAGTG CTCAGGGCGA GCGACAGATT CCAGTTCTTC AGACAAACAA TGGTCCAAGT 
CTAACAGGAT TGACTACTAT AGCAGCTCAT CTAGTCAAGC AAGCCAACAA AGAATATTTG 
CTGGGGAGTA CTGCAGAAGA AAAAGCAATC GTTCAGCAGT GGTTAGAATA CAGGGTCACT 
CAAGTAGATG GGCACTCCAG TAAAAATGAC ATCCACACAC TGTTGAAGGA TCTTAATTCA 
TATCTTGAAG ATAAAGTCTA CCTTACAGGG TATAACTTTA CATTAGCAGA TATACTATTG 
TACTATGGAC TTCATCGCTT TATAGTTGAC CTGACAGTTC AAGAAAAGGA GAAATATCTT 
AATGTGTCTC GCTGGTTTTG TCACATTCAG CATTATCCAG GCATCAGGCA ACATCTGTCT 
AGTGTTGTCT TCATCAAGAA CAGACTATAT ACTAATTCCC ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1E1 ( NM_001135650.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129122.1 Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07328
Clone ID Related Accession (Same CDS sequence)	NM_001135650.1 , NM_001135650.2
Accession Version	NM_001135650.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	eukaryotic translation elongation factor 1 epsilon-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AV714140.2, BP235196.1, CK002875.1 and AI394376.1. Transcript Variant: This variant (2) includes an alternate 3' terminal exon, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CK002875.1, SRR1163658.268590.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267756 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG CCGCAGAGTT GTCGCTACTG GAGAAGTCCC TGGGACTGAG TAAGGGGAAT 
AAATACAGTG CTCAGGGCGA GCGACAGATT CCAGTTCTTC AGACAAACAA TGGTCCAAGT 
CTAACAGGAT TGACTACTAT AGCAGCTCAT CTAGTCAAGC AAGCCAACAA AGAATATTTG 
CTGGGGAGTA CTGCAGAAGA AAAAGCAATC GTTCAGCAGT GGTTAGAATA CAGGGTCACT 
CAAGTAGATG GGCACTCCAG TAAAAATGAC ATCCACACAC TGTTGAAGGA TCTTAATTCA 
TATCTTGAAG ATAAAGTCTA CCTTACAGGG TATAACTTTA CATTAGCAGA TATACTATTG 
TACTATGGAC TTCATCGCTT TATAATAAGG AAGCTGAGGC ACACAGAGGT AGGGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129122.1
CDS	75..494
Translation	MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNSYLEDKVYLTGYNFTLADILLYYGLHRFIIRKLRHTEVGN

Target ORF information:

RefSeq Version	NM_001135650.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135650.1

ORF Insert Sequence:

ATGGCGGCGG CCGCAGAGTT GTCGCTACTG GAGAAGTCCC TGGGACTGAG TAAGGGGAAT 
AAATACAGTG CTCAGGGCGA GCGACAGATT CCAGTTCTTC AGACAAACAA TGGTCCAAGT 
CTAACAGGAT TGACTACTAT AGCAGCTCAT CTAGTCAAGC AAGCCAACAA AGAATATTTG 
CTGGGGAGTA CTGCAGAAGA AAAAGCAATC GTTCAGCAGT GGTTAGAATA CAGGGTCACT 
CAAGTAGATG GGCACTCCAG TAAAAATGAC ATCCACACAC TGTTGAAGGA TCTTAATTCA 
TATCTTGAAG ATAAAGTCTA CCTTACAGGG TATAACTTTA CATTAGCAGA TATACTATTG 
TACTATGGAC TTCATCGCTT TATAATAAGG AAGCTGAGGC ACACAGAGGT AGGGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EEF1E1 ( NM_001135650.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129122.1 Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07328
Clone ID Related Accession (Same CDS sequence)	NM_001135650.1 , NM_001135650.2
Accession Version	NM_001135650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 420bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	eukaryotic translation elongation factor 1 epsilon-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP235196.1, CK002875.1 and AI394376.1. On May 31, 2019 this sequence version replaced NM_001135650.1. Transcript Variant: This variant (2) includes an alternate 3' terminal exon, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138512.266434.1, SRR1163658.268590.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267756 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG CCGCAGAGTT GTCGCTACTG GAGAAGTCCC TGGGACTGAG TAAGGGGAAT 
AAATACAGTG CTCAGGGCGA GCGACAGATT CCAGTTCTTC AGACAAACAA TGGTCCAAGT 
CTAACAGGAT TGACTACTAT AGCAGCTCAT CTAGTCAAGC AAGCCAACAA AGAATATTTG 
CTGGGGAGTA CTGCAGAAGA AAAAGCAATC GTTCAGCAGT GGTTAGAATA CAGGGTCACT 
CAAGTAGATG GGCACTCCAG TAAAAATGAC ATCCACACAC TGTTGAAGGA TCTTAATTCA 
TATCTTGAAG ATAAAGTCTA CCTTACAGGG TATAACTTTA CATTAGCAGA TATACTATTG 
TACTATGGAC TTCATCGCTT TATAATAAGG AAGCTGAGGC ACACAGAGGT AGGGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129122.1
CDS	28..447
Translation	MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNSYLEDKVYLTGYNFTLADILLYYGLHRFIIRKLRHTEVGN

Target ORF information:

RefSeq Version	NM_001135650.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation elongation factor 1 epsilon 1 (EEF1E1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135650.2

ORF Insert Sequence:

ATGGCGGCGG CCGCAGAGTT GTCGCTACTG GAGAAGTCCC TGGGACTGAG TAAGGGGAAT 
AAATACAGTG CTCAGGGCGA GCGACAGATT CCAGTTCTTC AGACAAACAA TGGTCCAAGT 
CTAACAGGAT TGACTACTAT AGCAGCTCAT CTAGTCAAGC AAGCCAACAA AGAATATTTG 
CTGGGGAGTA CTGCAGAAGA AAAAGCAATC GTTCAGCAGT GGTTAGAATA CAGGGTCACT 
CAAGTAGATG GGCACTCCAG TAAAAATGAC ATCCACACAC TGTTGAAGGA TCTTAATTCA 
TATCTTGAAG ATAAAGTCTA CCTTACAGGG TATAACTTTA CATTAGCAGA TATACTATTG 
TACTATGGAC TTCATCGCTT TATAATAAGG AAGCTGAGGC ACACAGAGGT AGGGAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mapping the contact surfaces in the Lamin A:AIMP3 complex by hydrogen/deuterium exchange FT-ICR mass spectrometry.
PloS one12(8)e0181869(2017)
Tao Y,Fang P,Kim S,Guo M,Young NL,Marshall AG

Assembly of Multi-tRNA Synthetase Complex via Heterotetrameric Glutathione Transferase-homology Domains.
The Journal of biological chemistry290(49)29313-28(2015 Dec)
Cho HY,Maeng SJ,Cho HJ,Choi YS,Chung JM,Lee S,Kim HK,Kim JH,Eom CY,Kim YG,Guo M,Jung HS,Kang BS,Kim S

miR-543 and miR-590-3p regulate human mesenchymal stem cell aging via direct targeting of AIMP3/p18.
Age (Dordrecht, Netherlands)36(6)9724(2014)
Lee S,Yu KR,Ryu YS,Oh YS,Hong IS,Kim HS,Lee JY,Kim S,Seo KW,Kang KS

Identification of two novel BRCA1-partner genes in the DNA double-strand break repair pathway.
Breast cancer research and treatment141(3)515-22(2013 Oct)
Guglielmi C,Cerri I,Evangelista M,Collavoli A,Tancredi M,Aretini P,Caligo MA

Genetics of coronary artery calcification among African Americans, a meta-analysis.
BMC medical genetics1475(2013 Jul)
Wojczynski MK,Li M,Bielak LF,Kerr KF,Reiner AP,Wong ND,Yanek LR,Qu L,White CC,Lange LA,Ferguson JF,He J,Young T,Mosley TH,Smith JA,Kral BG,Guo X,Wong Q,Ganesh SK,Heckbert SR,Griswold ME,O'Leary DH,Budoff M,Carr JJ,Taylor HA Jr,Bluemke DA,Demissie S,Hwang SJ,Paltoo DN,Polak JF,Psaty BM,Becker DM,Province MA,Post WS,O'Donnell CJ,Wilson JG,Harris TB,Kavousi M,Cupples LA,Rotter JI,Fornage M,Becker LC,Peyser PA,Borecki IB,Reilly MP

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EEF1E1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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