SNAP29 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SNAP29
Entrez Gene ID	9342
Full Name	synaptosome associated protein 29
Synonyms	CEDNIK,SNAP-29
General protein information	Preferred Names synaptosome associated protein 29 Names synaptosomal-associated protein 29 soluble 29 kDa NSF attachment protein synaptosomal-associated protein, 29kD synaptosomal-associated protein, 29kDa synaptosome associated protein 29kDa vesicle-membrane fusion protein SNAP-29
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q11.21
Summary	This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	604202
Disorder Html:	Cerebral dysgenesis, neuropathy, ichthyosis, and palmoplantar keratoderma syndrome, 609528 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004782.3	NP_004773.1	synaptosomal-associated protein 29
NM_004782.4	NP_004773.1	synaptosomal-associated protein 29

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SNAP29	NP_001069427.1
Homo sapiens (human)	SNAP29	NP_004773.1
Mus musculus (house mouse)	Snap29	NP_075837.3
Danio rerio (zebrafish)	snap29	NP_001243185.1
Drosophila melanogaster (fruit fly)	usnp	NP_523831.1
Caenorhabditis elegans (roundworm)	snap-29	NP_498940.1
Xenopus tropicalis (tropical clawed frog)	snap29	NP_988988.1
	SNAP29	XP_001086227.1
Rattus norvegicus (Norway rat)	Snap29	NP_446262.3
Gallus gallus (chicken)	SNAP29	NP_001025823.1
Pan troglodytes (chimpanzee)	SNAP29	XP_514997.2
Canis lupus familiaris (dog)	SNAP29	XP_543568.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011135	XP_564390.3

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

An essential step of kinetochore formation controlled by the SNARE protein Snap29.
Morelli E, Mastrodonato V, Beznoussenko GV, Mironov AA, Tognon E, Vaccari T
The EMBO journal35(20)2223-2237(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Here, we identify a novel role for Snap29, an unconventional SNARE, in promoting kinetochore assembly during mitosis in Drosophila and human cells. Snap29 localizes to the outer kinetochore and prevents chromosome mis-segregation and the formation of cells with fragmented nuclei.
Title: An essential step of kinetochore formation controlled by the SNARE protein Snap29.

phenotypic variability in Arab families with c.223delG mutation affected by cerebral dysgenesis, neuropathy, ichthyosis and keratoderma syndrome
Title: New Arab family with cerebral dysgenesis, neuropathy, ichthyosis and keratoderma syndrome suggests a possible founder effect for the c.223delG mutation.

In mammalian cells, mutating the O-GlcNAc sites in SNAP-29, promotes the formation of a SNAP-29-containing SNARE complex, increases fusion between autophagosomes and endosomes/lysosomes, and promotes autophagic flux.
Title: O-GlcNAc-modification of SNAP-29 regulates autophagosome maturation.

support a role of Snap29 at key steps of membrane trafficking, and predict that signaling defects may contribute to the pathogenesis of cerebral dysgenesis
Title: Multiple functions of the SNARE protein Snap29 in autophagy, endocytic, and exocytic trafficking during epithelial formation in Drosophila.

This work implicates SNAP29 as a major modifier of variable expressivity in 22q11.2 DS patients.
Title: Hemizygous mutations in SNAP29 unmask autosomal recessive conditions and contribute to atypical findings in patients with 22q11.2DS.

The following SNAP29 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNAP29 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26530	NM_004782.4 Latest version!	Homo sapiens synaptosome associated protein 29 (SNAP29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu26530	NM_004782.3	Homo sapiens synaptosome associated protein 29 (SNAP29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SNAP29 ( NM_004782.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004773.1 Homo sapiens synaptosome associated protein 29 (SNAP29), mRNA.

Price & Availability↑

CloneID	OHu26530
Clone ID Related Accession (Same CDS sequence)	NM_004782.3 , NM_004782.4
Accession Version	NM_004782.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	synaptosomal-associated protein 29
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG773022.1, AF115436.1, AC007308.13 and CA427899.1. This sequence is a reference standard in the RefSeqGene project. On Oct 8, 2008 this sequence version replaced NM_004782.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.243742.1, SRR1660805.24095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGCTT ACCCTAAAAG CTACAATCCG TTCGACGACG ACGGGGAGGA CGAAGGCGCC 
CGGCCGGCCC CTTGGAGGGA CGCCCGAGAC CTCCCCGACG GGCCCGACGC GCCCGCGGAC 
AGGCAGCAGT ACTTGCGGCA GGAGGTCCTC CGCAGGGCTG AGGCCACGGC CGCCAGCACC 
AGCAGGTCCC TGGCCCTCAT GTACGAGTCC GAGAAGGTTG GGGTCGCCTC TTCCGAGGAG 
CTCGCCCGTC AGCGAGGAGT CCTGGAGCGC ACAGAGAAGA TGGTGGACAA GATGGACCAA 
GATTTGAAGA TCAGCCAGAA ACACATCAAT AGCATTAAGA GCGTGTTTGG GGGGCTGGTC 
AATTACTTCA AATCCAAACC AGTAGAGACC CCACCTGAAC AGAATGGCAC CCTCACCTCC 
CAGCCCAACA ACAGATTGAA AGAAGCTATA AGTACAAGTA AAGAACAGGA AGCAAAGTAC 
CAGGCCAGCC ACCCAAACCT TAGAAAGCTG GATGATACAG ACCCTGTCCC CAGAGGGGCT 
GGTTCTGCCA TGAGTACTGA TGCTTACCCA AAGAACCCAC ACCTTCGAGC CTATCACCAG 
AAGATCGACA GCAACCTAGA TGAGCTGTCC ATGGGACTGG GTCGTCTGAA GGACATAGCC 
CTGGGGATGC AGACAGAAAT TGAGGAGCAA GATGACATTC TTGACCGGCT GACAACCAAA 
GTGGACAAGT TAGATGTCAA CATAAAAAGC ACAGAAAGAA AAGTTCGACA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004773.1
CDS	108..884
Translation	MSAYPKSYNPFDDDGEDEGARPAPWRDARDLPDGPDAPADRQQYLRQEVLRRAEATAASTSRSLALMYESEKVGVASSEELARQRGVLERTEKMVDKMDQDLKISQKHINSIKSVFGGLVNYFKSKPVETPPEQNGTLTSQPNNRLKEAISTSKEQEAKYQASHPNLRKLDDTDPVPRGAGSAMSTDAYPKNPHLRAYHQKIDSNLDELSMGLGRLKDIALGMQTEIEEQDDILDRLTTKVDKLDVNIKSTERKVRQL

Target ORF information:

RefSeq Version	NM_004782.3
Organism	Homo sapiens(human)
Definition	Homo sapiens synaptosome associated protein 29 (SNAP29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004782.3

ORF Insert Sequence:

ATGTCAGCTT ACCCTAAAAG CTACAATCCG TTCGACGACG ACGGGGAGGA CGAAGGCGCC 
CGGCCGGCCC CTTGGAGGGA CGCCCGAGAC CTCCCCGACG GGCCCGACGC GCCCGCGGAC 
AGGCAGCAGT ACTTGCGGCA GGAGGTCCTC CGCAGGGCTG AGGCCACGGC CGCCAGCACC 
AGCAGGTCCC TGGCCCTCAT GTACGAGTCC GAGAAGGTTG GGGTCGCCTC TTCCGAGGAG 
CTCGCCCGTC AGCGAGGAGT CCTGGAGCGC ACAGAGAAGA TGGTGGACAA GATGGACCAA 
GATTTGAAGA TCAGCCAGAA ACACATCAAT AGCATTAAGA GCGTGTTTGG GGGGCTGGTC 
AATTACTTCA AATCCAAACC AGTAGAGACC CCACCTGAAC AGAATGGCAC CCTCACCTCC 
CAGCCCAACA ACAGATTGAA AGAAGCTATA AGTACAAGTA AAGAACAGGA AGCAAAGTAC 
CAGGCCAGCC ACCCAAACCT TAGAAAGCTG GATGATACAG ACCCTGTCCC CAGAGGGGCT 
GGTTCTGCCA TGAGTACTGA TGCTTACCCA AAGAACCCAC ACCTTCGAGC CTATCACCAG 
AAGATCGACA GCAACCTAGA TGAGCTGTCC ATGGGACTGG GTCGTCTGAA GGACATAGCC 
CTGGGGATGC AGACAGAAAT TGAGGAGCAA GATGACATTC TTGACCGGCT GACAACCAAA 
GTGGACAAGT TAGATGTCAA CATAAAAAGC ACAGAAAGAA AAGTTCGACA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNAP29 ( NM_004782.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004773.1 Homo sapiens synaptosome associated protein 29 (SNAP29), mRNA.

Price & Availability↑

CloneID	OHu26530
Clone ID Related Accession (Same CDS sequence)	NM_004782.3 , NM_004782.4
Accession Version	NM_004782.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	synaptosomal-associated protein 29
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG773022.1, AF115436.1, AC007308.13 and CA427899.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004782.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.113951.1, SRR1660809.243742.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000215730.12/ ENSP00000215730.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCAGCTT ACCCTAAAAG CTACAATCCG TTCGACGACG ACGGGGAGGA CGAAGGCGCC 
CGGCCGGCCC CTTGGAGGGA CGCCCGAGAC CTCCCCGACG GGCCCGACGC GCCCGCGGAC 
AGGCAGCAGT ACTTGCGGCA GGAGGTCCTC CGCAGGGCTG AGGCCACGGC CGCCAGCACC 
AGCAGGTCCC TGGCCCTCAT GTACGAGTCC GAGAAGGTTG GGGTCGCCTC TTCCGAGGAG 
CTCGCCCGTC AGCGAGGAGT CCTGGAGCGC ACAGAGAAGA TGGTGGACAA GATGGACCAA 
GATTTGAAGA TCAGCCAGAA ACACATCAAT AGCATTAAGA GCGTGTTTGG GGGGCTGGTC 
AATTACTTCA AATCCAAACC AGTAGAGACC CCACCTGAAC AGAATGGCAC CCTCACCTCC 
CAGCCCAACA ACAGATTGAA AGAAGCTATA AGTACAAGTA AAGAACAGGA AGCAAAGTAC 
CAGGCCAGCC ACCCAAACCT TAGAAAGCTG GATGATACAG ACCCTGTCCC CAGAGGGGCT 
GGTTCTGCCA TGAGTACTGA TGCTTACCCA AAGAACCCAC ACCTTCGAGC CTATCACCAG 
AAGATCGACA GCAACCTAGA TGAGCTGTCC ATGGGACTGG GTCGTCTGAA GGACATAGCC 
CTGGGGATGC AGACAGAAAT TGAGGAGCAA GATGACATTC TTGACCGGCT GACAACCAAA 
GTGGACAAGT TAGATGTCAA CATAAAAAGC ACAGAAAGAA AAGTTCGACA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004773.1
CDS	105..881
Translation	MSAYPKSYNPFDDDGEDEGARPAPWRDARDLPDGPDAPADRQQYLRQEVLRRAEATAASTSRSLALMYESEKVGVASSEELARQRGVLERTEKMVDKMDQDLKISQKHINSIKSVFGGLVNYFKSKPVETPPEQNGTLTSQPNNRLKEAISTSKEQEAKYQASHPNLRKLDDTDPVPRGAGSAMSTDAYPKNPHLRAYHQKIDSNLDELSMGLGRLKDIALGMQTEIEEQDDILDRLTTKVDKLDVNIKSTERKVRQL

Target ORF information:

RefSeq Version	NM_004782.4
Organism	Homo sapiens(human)
Definition	Homo sapiens synaptosome associated protein 29 (SNAP29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004782.4

ORF Insert Sequence:

ATGTCAGCTT ACCCTAAAAG CTACAATCCG TTCGACGACG ACGGGGAGGA CGAAGGCGCC 
CGGCCGGCCC CTTGGAGGGA CGCCCGAGAC CTCCCCGACG GGCCCGACGC GCCCGCGGAC 
AGGCAGCAGT ACTTGCGGCA GGAGGTCCTC CGCAGGGCTG AGGCCACGGC CGCCAGCACC 
AGCAGGTCCC TGGCCCTCAT GTACGAGTCC GAGAAGGTTG GGGTCGCCTC TTCCGAGGAG 
CTCGCCCGTC AGCGAGGAGT CCTGGAGCGC ACAGAGAAGA TGGTGGACAA GATGGACCAA 
GATTTGAAGA TCAGCCAGAA ACACATCAAT AGCATTAAGA GCGTGTTTGG GGGGCTGGTC 
AATTACTTCA AATCCAAACC AGTAGAGACC CCACCTGAAC AGAATGGCAC CCTCACCTCC 
CAGCCCAACA ACAGATTGAA AGAAGCTATA AGTACAAGTA AAGAACAGGA AGCAAAGTAC 
CAGGCCAGCC ACCCAAACCT TAGAAAGCTG GATGATACAG ACCCTGTCCC CAGAGGGGCT 
GGTTCTGCCA TGAGTACTGA TGCTTACCCA AAGAACCCAC ACCTTCGAGC CTATCACCAG 
AAGATCGACA GCAACCTAGA TGAGCTGTCC ATGGGACTGG GTCGTCTGAA GGACATAGCC 
CTGGGGATGC AGACAGAAAT TGAGGAGCAA GATGACATTC TTGACCGGCT GACAACCAAA 
GTGGACAAGT TAGATGTCAA CATAAAAAGC ACAGAAAGAA AAGTTCGACA ACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

An essential step of kinetochore formation controlled by the SNARE protein Snap29.
The EMBO journal35(20)2223-2237(2016 10)
Morelli E,Mastrodonato V,Beznoussenko GV,Mironov AA,Tognon E,Vaccari T

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SNAP29 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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