LRAT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LRAT
Entrez Gene ID	9227
Full Name	lecithin retinol acyltransferase
Synonyms	LCA14
General protein information	Preferred Names lecithin retinol acyltransferase Names lecithin retinol acyltransferase lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q32.1
Summary	The protein encoded by this gene localizes to the endoplasmic reticulum, where it catalyzes the esterification of all-trans-retinol into all-trans-retinyl ester. This reaction is an important step in vitamin A metabolism in the visual system. Mutations in this gene have been associated with early-onset severe retinal dystrophy and Leber congenital amaurosis 14. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014].
Disorder MIM:	604863
Disorder Html:	Retinal dystrophy, early-onset severe, 613341 (3); Leber congenital amaurosis 14, 613341 (3); Retinitis pigmentosa, juvenile, 613341 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004744.4	NP_004735.2	lecithin retinol acyltransferase precursor
XM_006714412.2	XP_006714475.1	lecithin retinol acyltransferase isoform X1
NM_004744.5	NP_004735.2	lecithin retinol acyltransferase precursor
NM_001301645.1	NP_001288574.1	lecithin retinol acyltransferase precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	LRAT	XP_539777.2
Gallus gallus (chicken)	LRAT	XP_420371.2
Xenopus tropicalis (tropical clawed frog)	lrat	NP_989141.1
Bos taurus (cattle)	LRAT	NP_803469.1
Rattus norvegicus (Norway rat)	Lrat	NP_071616.1
Danio rerio (zebrafish)	lrata	NP_001191060.1
	LRAT	XP_001092118.1
Xenopus tropicalis (tropical clawed frog)	LOC100485002	XP_002933533.1
Homo sapiens (human)	LRAT	NP_004735.2
Pan troglodytes (chimpanzee)	LRAT	XP_526710.2
Mus musculus (house mouse)	Lrat	NP_076113.1
Danio rerio (zebrafish)	lratb	XP_001921190.2

Gene Ontology
Bibliography

Related articles in PubMed

Impact of LCA-Associated E14L LRAT Mutation on Protein Stability and Retinoid Homeostasis.
Chelstowska S, Widjaja-Adhi MAK, Silvaroli JA, Golczak M
Biochemistry56(34)4489-4499(2017 08)

LRAT-specific domain facilitates vitamin A metabolism by domain swapping in HRASLS3.
Golczak M, Sears AE, Kiser PD, Palczewski K
Nature chemical biology11(1)26-32(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

instability of LRAT(E14L) did not abrogate the production of the visual chromophore in a cell-based assay. Instead, expression of LRAT(E14L) led to a rapid increase in cellular levels of retinoic acid upon retinoid supplementation.
Title: Impact of LCA-Associated E14L LRAT Mutation on Protein Stability and Retinoid Homeostasis.

These findings reveal structural adaptation that facilitates selective catalysis and mechanism responsible for diverse substrate specificity within the LRAT-like enzyme family
Title: LRAT-specific domain facilitates vitamin A metabolism by domain swapping in HRASLS3.

LRAT hypermethylation was associated with decreased mRNA levels in colorectal cancer clinical specimens.
Title: High incidence of LRAT promoter hypermethylation in colorectal cancer correlates with tumor stage.

lecithin retinol acyltransferase affects all-trans retinoic acid levels and has a role in retinoid sensitivity in malignant melanoma cells.
Title: Knockdown of lecithin retinol acyltransferase increases all-trans retinoic acid levels and restores retinoid sensitivity in malignant melanoma cells.

Lecithin-retinol acyltransferase is a thermostable and highly active enzyme with a likely mode of interfacial activation.
Title: Enzymatic activity of Lecithin:retinol acyltransferase: a thermostable and highly active enzyme with a likely mode of interfacial activation.

The following LRAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LRAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18303	NM_004744.5 Latest version!	Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18303	NM_001301645.1 Latest version!	Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu18303	XM_006714412.2 Latest version!	Homo sapiens lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) (LRAT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18303	NM_004744.4	Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LRAT ( NM_004744.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004735.2 Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18303
Clone ID Related Accession (Same CDS sequence)	NM_004744.4 , XM_006714412.2 , NM_004744.5 , NM_001301645.1
Accession Version	NM_004744.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	lecithin retinol acyltransferase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292598.1, AY546086.1 and DB364008.1. This sequence is a reference standard in the RefSeqGene project. On Aug 27, 2014 this sequence version replaced NM_004744.3. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145313, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC 
AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT 
GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC 
TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC 
CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC 
GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT 
AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG 
GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC 
TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG 
TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC 
TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT 
TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004735.2
CDS	254..946
Translation	MKNPMLEVVSLLLEKLLLISNFTLFSSGAAGEDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVLGLASIVCTGLVSYTTLPAIFIPFFLWMAG

Target ORF information:

RefSeq Version	NM_004744.4
Organism	Homo sapiens(human)
Definition	Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004744.4

ORF Insert Sequence:

ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC 
AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT 
GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC 
TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC 
CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC 
GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT 
AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG 
GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC 
TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG 
TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC 
TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT 
TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRAT ( NM_004744.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004735.2 Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18303
Clone ID Related Accession (Same CDS sequence)	NM_004744.4 , XM_006714412.2 , NM_004744.5 , NM_001301645.1
Accession Version	NM_004744.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	lecithin retinol acyltransferase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292598.1, AY546086.1, DB364008.1 and AC009567.8. This sequence is a reference standard in the RefSeqGene project. On Nov 24, 2018 this sequence version replaced NM_004744.4. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY546086.1, AF071510.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145313, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336356.4/ ENSP00000337224.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC 
AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT 
GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC 
TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC 
CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC 
GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT 
AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG 
GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC 
TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG 
TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC 
TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT 
TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004735.2
CDS	225..917
Translation	MKNPMLEVVSLLLEKLLLISNFTLFSSGAAGEDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVLGLASIVCTGLVSYTTLPAIFIPFFLWMAG

Target ORF information:

RefSeq Version	NM_004744.5
Organism	Homo sapiens(human)
Definition	Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004744.5

ORF Insert Sequence:

ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC 
AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT 
GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC 
TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC 
CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC 
GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT 
AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG 
GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC 
TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG 
TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC 
TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT 
TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRAT ( NM_001301645.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288574.1 Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu18303
Clone ID Related Accession (Same CDS sequence)	NM_004744.4 , XM_006714412.2 , NM_004744.5 , NM_001301645.1
Accession Version	NM_001301645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	lecithin retinol acyltransferase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC031053.1, AY546086.1 and DB364008.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031053.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC 
AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT 
GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC 
TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC 
CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC 
GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT 
AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG 
GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC 
TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG 
TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC 
TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT 
TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288574.1
CDS	60..752
Translation	MKNPMLEVVSLLLEKLLLISNFTLFSSGAAGEDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVLGLASIVCTGLVSYTTLPAIFIPFFLWMAG

Target ORF information:

RefSeq Version	NM_001301645.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lecithin retinol acyltransferase (LRAT), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301645.1

ORF Insert Sequence:

ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC 
AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT 
GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC 
TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC 
CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC 
GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT 
AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG 
GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC 
TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG 
TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC 
TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT 
TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LRAT ( XM_006714412.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714475.1 Homo sapiens lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) (LRAT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu18303
Clone ID Related Accession (Same CDS sequence)	NM_004744.4 , XM_006714412.2 , NM_004744.5 , NM_001301645.1
Accession Version	XM_006714412.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	lecithin retinol acyltransferase isoform X1
Comment

ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC 
AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT 
GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC 
TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC 
CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC 
GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT 
AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG 
GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC 
TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG 
TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC 
TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT 
TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714475.1
Translation	MKNPMLEVVSLLLEKLLLISNFTLFSSGAAGEDKGRNSFYETSSFHRGDVLEVPRTHLTHYGIYLGDNRVAHMMPDILLALTDDMGRTQKVVSNKRLILGVIVKVASIRVDTVEDFAYGANILVNHLDESLQKKALLNEEVARRAEKLLGFTPYSLLWNNCEHFVTYCRYGTPISPQSDKFCETVKIIIRDQRSVLASAVLGLASIVCTGLVSYTTLPAIFIPFFLWMAG

Target ORF information:

RefSeq Version	XM_006714412.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase) (LRAT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714412.2

ORF Insert Sequence:

ATGAAGAACC CCATGCTGGA GGTGGTGTCT TTACTACTGG AGAAGCTGCT CCTCATCTCC 
AACTTCACGC TCTTTAGTTC GGGCGCCGCG GGCGAAGACA AAGGGAGGAA CAGTTTTTAT 
GAAACCAGCT CTTTCCACCG AGGCGACGTG CTGGAGGTGC CCCGGACCCA CCTGACCCAC 
TATGGCATCT ACCTAGGAGA CAACCGTGTT GCCCACATGA TGCCCGACAT CCTGTTGGCC 
CTGACAGACG ACATGGGGCG CACGCAGAAG GTGGTCTCCA ACAAGCGTCT CATCCTGGGC 
GTTATTGTCA AAGTGGCCAG CATCCGCGTG GACACAGTGG AGGACTTCGC CTACGGAGCT 
AACATCCTGG TCAATCACCT GGACGAGTCC CTCCAGAAAA AGGCACTGCT CAACGAGGAG 
GTGGCGCGGA GGGCTGAAAA GCTGCTGGGC TTTACCCCCT ACAGCCTGCT GTGGAACAAC 
TGCGAGCACT TCGTGACCTA CTGCAGATAT GGCACCCCGA TCAGTCCCCA GTCCGACAAG 
TTTTGTGAGA CTGTGAAGAT AATTATTCGT GATCAGAGAA GTGTTCTTGC TTCAGCAGTC 
TTGGGATTGG CGTCTATAGT CTGTACGGGC TTGGTATCAT ACACTACCCT TCCTGCAATT 
TTTATTCCAT TCTTCCTATG GATGGCTGGC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impact of LCA-Associated E14L LRAT Mutation on Protein Stability and Retinoid Homeostasis.
Biochemistry56(34)4489-4499(2017 08)
Chelstowska S,Widjaja-Adhi MAK,Silvaroli JA,Golczak M

LRAT-specific domain facilitates vitamin A metabolism by domain swapping in HRASLS3.
Nature chemical biology11(1)26-32(2015 Jan)
Golczak M,Sears AE,Kiser PD,Palczewski K

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LRAT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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