CD3E cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD3E
Entrez Gene ID	916
Full Name	CD3e molecule
Synonyms	IMD18,T3E,TCRE
General protein information	Preferred Names CD3e molecule Names T-cell surface glycoprotein CD3 epsilon chain CD3-epsilon CD3e antigen, epsilon polypeptide (TiT3 complex) CD3e molecule, epsilon (CD3-TCR complex) T-cell antigen receptor complex, epsilon subunit of T3 T-cell surface antigen T3/Leu-4 epsilon chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q23.3
Summary	The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008].
Disorder MIM:	186830
Disorder Html:	Immunodeficiency 18, 615615 (3); Immunodeficiency 18, SCID variant, 615615 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000733.4	NP_000724.1	T-cell surface glycoprotein CD3 epsilon chain precursor
NM_000733.3	NP_000724.1	T-cell surface glycoprotein CD3 epsilon chain precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CD3E	NP_996787.1
Homo sapiens (human)	CD3E	NP_000724.1
Canis lupus familiaris (dog)	CD3E	NP_001003379.1
Mus musculus (house mouse)	Cd3e	NP_031674.1
Xenopus tropicalis (tropical clawed frog)	cd3e	XP_002932946.1
Pan troglodytes (chimpanzee)	CD3E	XP_001160742.1
	CD3E	XP_001097204.1
Bos taurus (cattle)	CD3E	NP_776436.1
Rattus norvegicus (Norway rat)	Cd3e	NP_001101610.1

Gene Ontology
Bibliography

Related articles in PubMed

A novel pathogenic frameshift variant of CD3E gene in two T-B+ NK+ SCID patients from Turkey.
Firtina S, Ng YY, Ng OH, Nepesov S, Yesilbas O, Kilercik M, Burtecene N, Cinar S, Camcioglu Y, Ozbek U, Sayitoglu M
Immunogenetics69(10)653-659(2017 10)

Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling.
Li L, Guo X, Shi X, Li C, Wu W, Yan C, Wang H, Li H, Xu C
Proceedings of the National Academy of Sciences of the United States of America114(29)E5891-E5899(2017 Jul)

The Stalk Domain of NKp30 Contributes to Ligand Binding and Signaling of a Preassembled NKp30-CD3? Complex.
Memmer S, Weil S, Beyer S, Z?ller T, Peters E, Hartmann J, Steinle A, Koch J
The Journal of biological chemistry291(49)25427-25438(2016 Dec)

Change in CD3?-chain expression is an independent predictor of disease status in head and neck cancer patients.
Upreti D, Zhang ML, Bykova E, Kung SK, Pathak KA
International journal of cancer139(1)122-9(2016 Jul)

The Transmembrane Domain of HIV-1 gp41 Inhibits T-Cell Activation by Targeting Multiple T-Cell Receptor Complex Components through Its GxxxG Motif.
Rotem E, Reuven EM, Klug YA, Shai Y
Biochemistry55(7)1049-57(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel pathogenic frameshift variant of CD3E gene has been found in two unrelated T-B+ NK+ severe combined immunodeficiency infants from Turkey.
Title: A novel pathogenic frameshift variant of CD3E gene in two T-B+ NK+ SCID patients from Turkey.

the stalk domains of NKp30 and NKp46, another NCR employing CD3zeta for signaling, were not exchangeable without drastic deficiencies in folding, plasma membrane targeting, and/or ligand-induced receptor signaling.
Title: The Stalk Domain of NKp30 Contributes to Ligand Binding and Signaling of a Preassembled NKp30-CD3? Complex.

increase. Our results indicate that the change in CD3zeta-chain expression from the baseline is an independent predictor of residual and recurrent head and neck squamous cell carcinoma.
Title: Change in CD3?-chain expression is an independent predictor of disease status in head and neck cancer patients.

Data suggest that HIV-1 gp41 transmembrane domain (TMD) directly interacts with TMDs of the T-cell receptor and it's CD3-antigen co-receptors (delta, gamma, and epsilon); these interactions appear to be involved in immune evasion mechanism of HIV-1.
Title: The Transmembrane Domain of HIV-1 gp41 Inhibits T-Cell Activation by Targeting Multiple T-Cell Receptor Complex Components through Its GxxxG Motif.

The inducible recruitment of WASp to the TCR-CD3 complex is partially dependent of tyrosine phosphorylation of Cd3e.
Title: Evidence for inducible recruitment of Wiskott-Aldrich syndrome protein to T cell receptor-CD3 complex in Jurkat T cells.

The following CD3E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD3E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19406	NM_000733.4 Latest version!	Homo sapiens CD3e molecule (CD3E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu19406	NM_000733.3	Homo sapiens CD3e molecule (CD3E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD3E ( NM_000733.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000724.1 Homo sapiens CD3e molecule (CD3E), mRNA.

Price & Availability↑

CloneID	OHu19406
Clone ID Related Accession (Same CDS sequence)	NM_000733.4 , NM_000733.3
Accession Version	NM_000733.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	T-cell surface glycoprotein CD3 epsilon chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP366535.1, AK292612.1 and BC049847.1. On Aug 31, 2019 this sequence version replaced NM_000733.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292612.1, BC049847.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCGG GCACTCACTG GAGAGTTCTG GGCCTCTGCC TCTTATCAGT TGGCGTTTGG 
GGGCAAGATG GTAATGAAGA AATGGGTGGT ATTACACAGA CACCATATAA AGTCTCCATC 
TCTGGAACCA CAGTAATATT GACATGCCCT CAGTATCCTG GATCTGAAAT ACTATGGCAA 
CACAATGATA AAAACATAGG CGGTGATGAG GATGATAAAA ACATAGGCAG TGATGAGGAT 
CACCTGTCAC TGAAGGAATT TTCAGAATTG GAGCAAAGTG GTTATTATGT CTGCTACCCC 
AGAGGAAGCA AACCAGAAGA TGCGAACTTT TATCTCTACC TGAGGGCAAG AGTGTGTGAG 
AACTGCATGG AGATGGATGT GATGTCGGTG GCCACAATTG TCATAGTGGA CATCTGCATC 
ACTGGGGGCT TGCTGCTGCT GGTTTACTAC TGGAGCAAGA ATAGAAAGGC CAAGGCCAAG 
CCTGTGACAC GAGGAGCGGG TGCTGGCGGC AGGCAAAGGG GACAAAACAA GGAGAGGCCA 
CCACCTGTTC CCAACCCAGA CTATGAGCCC ATCCGGAAAG GCCAGCGGGA CCTGTATTCT 
GGCCTGAATC AGAGACGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000724.1
CDS	107..730
Translation	MQSGTHWRVLGLCLLSVGVWGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMDVMSVATIVIVDICITGGLLLLVYYWSKNRKAKAKPVTRGAGAGGRQRGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRRI

Target ORF information:

RefSeq Version	NM_000733.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CD3e molecule (CD3E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000733.4

ORF Insert Sequence:

ATGCAGTCGG GCACTCACTG GAGAGTTCTG GGCCTCTGCC TCTTATCAGT TGGCGTTTGG 
GGGCAAGATG GTAATGAAGA AATGGGTGGT ATTACACAGA CACCATATAA AGTCTCCATC 
TCTGGAACCA CAGTAATATT GACATGCCCT CAGTATCCTG GATCTGAAAT ACTATGGCAA 
CACAATGATA AAAACATAGG CGGTGATGAG GATGATAAAA ACATAGGCAG TGATGAGGAT 
CACCTGTCAC TGAAGGAATT TTCAGAATTG GAGCAAAGTG GTTATTATGT CTGCTACCCC 
AGAGGAAGCA AACCAGAAGA TGCGAACTTT TATCTCTACC TGAGGGCAAG AGTGTGTGAG 
AACTGCATGG AGATGGATGT GATGTCGGTG GCCACAATTG TCATAGTGGA CATCTGCATC 
ACTGGGGGCT TGCTGCTGCT GGTTTACTAC TGGAGCAAGA ATAGAAAGGC CAAGGCCAAG 
CCTGTGACAC GAGGAGCGGG TGCTGGCGGC AGGCAAAGGG GACAAAACAA GGAGAGGCCA 
CCACCTGTTC CCAACCCAGA CTATGAGCCC ATCCGGAAAG GCCAGCGGGA CCTGTATTCT 
GGCCTGAATC AGAGACGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD3E ( NM_000733.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000724.1 Homo sapiens CD3e molecule (CD3E), mRNA.

Price & Availability↑

CloneID	OHu19406
Clone ID Related Accession (Same CDS sequence)	NM_000733.4 , NM_000733.3
Accession Version	NM_000733.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Homo sapiens(human)
Product	T-cell surface glycoprotein CD3 epsilon chain precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP366535.1, AK292612.1 and BC049847.1. This sequence is a reference standard in the RefSeqGene project. On Jan 31, 2008 this sequence version replaced NM_000733.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC049847.1, AK292612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGTCGG GCACTCACTG GAGAGTTCTG GGCCTCTGCC TCTTATCAGT TGGCGTTTGG 
GGGCAAGATG GTAATGAAGA AATGGGTGGT ATTACACAGA CACCATATAA AGTCTCCATC 
TCTGGAACCA CAGTAATATT GACATGCCCT CAGTATCCTG GATCTGAAAT ACTATGGCAA 
CACAATGATA AAAACATAGG CGGTGATGAG GATGATAAAA ACATAGGCAG TGATGAGGAT 
CACCTGTCAC TGAAGGAATT TTCAGAATTG GAGCAAAGTG GTTATTATGT CTGCTACCCC 
AGAGGAAGCA AACCAGAAGA TGCGAACTTT TATCTCTACC TGAGGGCAAG AGTGTGTGAG 
AACTGCATGG AGATGGATGT GATGTCGGTG GCCACAATTG TCATAGTGGA CATCTGCATC 
ACTGGGGGCT TGCTGCTGCT GGTTTACTAC TGGAGCAAGA ATAGAAAGGC CAAGGCCAAG 
CCTGTGACAC GAGGAGCGGG TGCTGGCGGC AGGCAAAGGG GACAAAACAA GGAGAGGCCA 
CCACCTGTTC CCAACCCAGA CTATGAGCCC ATCCGGAAAG GCCAGCGGGA CCTGTATTCT 
GGCCTGAATC AGAGACGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000724.1
CDS	257..880
Translation	MQSGTHWRVLGLCLLSVGVWGQDGNEEMGGITQTPYKVSISGTTVILTCPQYPGSEILWQHNDKNIGGDEDDKNIGSDEDHLSLKEFSELEQSGYYVCYPRGSKPEDANFYLYLRARVCENCMEMDVMSVATIVIVDICITGGLLLLVYYWSKNRKAKAKPVTRGAGAGGRQRGQNKERPPPVPNPDYEPIRKGQRDLYSGLNQRRI

Target ORF information:

RefSeq Version	NM_000733.3
Organism	Homo sapiens(human)
Definition	Homo sapiens CD3e molecule (CD3E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000733.3

ORF Insert Sequence:

ATGCAGTCGG GCACTCACTG GAGAGTTCTG GGCCTCTGCC TCTTATCAGT TGGCGTTTGG 
GGGCAAGATG GTAATGAAGA AATGGGTGGT ATTACACAGA CACCATATAA AGTCTCCATC 
TCTGGAACCA CAGTAATATT GACATGCCCT CAGTATCCTG GATCTGAAAT ACTATGGCAA 
CACAATGATA AAAACATAGG CGGTGATGAG GATGATAAAA ACATAGGCAG TGATGAGGAT 
CACCTGTCAC TGAAGGAATT TTCAGAATTG GAGCAAAGTG GTTATTATGT CTGCTACCCC 
AGAGGAAGCA AACCAGAAGA TGCGAACTTT TATCTCTACC TGAGGGCAAG AGTGTGTGAG 
AACTGCATGG AGATGGATGT GATGTCGGTG GCCACAATTG TCATAGTGGA CATCTGCATC 
ACTGGGGGCT TGCTGCTGCT GGTTTACTAC TGGAGCAAGA ATAGAAAGGC CAAGGCCAAG 
CCTGTGACAC GAGGAGCGGG TGCTGGCGGC AGGCAAAGGG GACAAAACAA GGAGAGGCCA 
CCACCTGTTC CCAACCCAGA CTATGAGCCC ATCCGGAAAG GCCAGCGGGA CCTGTATTCT 
GGCCTGAATC AGAGACGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel pathogenic frameshift variant of CD3E gene in two T-B+ NK+ SCID patients from Turkey.
Immunogenetics69(10)653-659(2017 10)
Firtina S,Ng YY,Ng OH,Nepesov S,Yesilbas O,Kilercik M,Burtecene N,Cinar S,Camcioglu Y,Ozbek U,Sayitoglu M

Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling.
Proceedings of the National Academy of Sciences of the United States of America114(29)E5891-E5899(2017 Jul)
Li L,Guo X,Shi X,Li C,Wu W,Yan C,Wang H,Li H,Xu C

The Stalk Domain of NKp30 Contributes to Ligand Binding and Signaling of a Preassembled NKp30-CD3? Complex.
The Journal of biological chemistry291(49)25427-25438(2016 Dec)
Memmer S,Weil S,Beyer S,Z?ller T,Peters E,Hartmann J,Steinle A,Koch J

Change in CD3?-chain expression is an independent predictor of disease status in head and neck cancer patients.
International journal of cancer139(1)122-9(2016 Jul)
Upreti D,Zhang ML,Bykova E,Kung SK,Pathak KA

The Transmembrane Domain of HIV-1 gp41 Inhibits T-Cell Activation by Targeting Multiple T-Cell Receptor Complex Components through Its GxxxG Motif.
Biochemistry55(7)1049-57(2016 Feb)
Rotem E,Reuven EM,Klug YA,Shai Y

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CD3E cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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