CD3E cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CD3E
Entrez Gene ID	281054
Full Name	CD3e molecule
General protein information	Preferred Names CD3e molecule Names T-cell surface glycoprotein CD3 epsilon chain CD3e molecule, epsilon (CD3-TCR complex) antigen CD3E, epsilon polypeptide (TiT3 complex)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024974979.1	XP_024830747.1	T-cell surface glycoprotein CD3 epsilon chain isoform X1
NM_174011.3	NP_776436.1	T-cell surface glycoprotein CD3 epsilon chain precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CD3E	NP_996787.1
Homo sapiens (human)	CD3E	NP_000724.1
Canis lupus familiaris (dog)	CD3E	NP_001003379.1
Mus musculus (house mouse)	Cd3e	NP_031674.1
Xenopus tropicalis (tropical clawed frog)	cd3e	XP_002932946.1
Pan troglodytes (chimpanzee)	CD3E	XP_001160742.1
	CD3E	XP_001097204.1
Bos taurus (cattle)	CD3E	NP_776436.1
Rattus norvegicus (Norway rat)	Cd3e	NP_001101610.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cloning, sequencing and expression of the bovine CD3 epsilon and TCR-zeta chains, two invariant components of the T-cell receptor complex.
Hagens G, Galley Y, Glaser I, Davis WC, Baldwin CL, Clevers H, Dobbelaere DA
Gene169(2)165-71(1996 Mar)

Identification of a bovine surface antigen uniquely expressed on CD4-CD8- T cell receptor gamma/delta+ T lymphocytes.
Clevers H, MacHugh ND, Bensaid A, Dunlap S, Baldwin CL, Kaushal A, Iams K, Howard CJ, Morrison WI
European journal of immunology20(4)809-17(1990 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CD3E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CD3E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa26203	NM_174011.3 Latest version!	Bos taurus CD3e molecule (CD3E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa26203	XM_024974979.1 Latest version!	Bos taurus CD3e molecule (CD3E), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CD3E ( NM_174011.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776436.1 Bos taurus CD3e molecule (CD3E), mRNA.

Price & Availability↑

CloneID	OBa26203
Clone ID Related Accession (Same CDS sequence)	XM_024974979.1 , NM_174011.3
Accession Version	NM_174011.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Bos taurus(cattle)
Product	T-cell surface glycoprotein CD3 epsilon chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC102243.1. On Aug 28, 2005 this sequence version replaced NM_174011.2. ##Evidence-Data-START## Transcript exon combination :: BC102243.1, EH172081.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGCAGTCGG GGAATCTCTG GCGAGCTCTG GGACTCTGCC TCTTATTAGT TGGTGCTTGG 
GCACAAGATG CTGATGAACA GAAACCATAT GAAGTCTCCA TCTCTGGAAA CACTGTTGAG 
CTGACATGCC CTAGAGAGTT TGAAGGTGAA ATACATTGGA AACAAAATGA TGAACAAATG 
AAAGGCTATA CTGGGAAACA GCTGTTACTG GAAAACTTTT CAGAAATGGA TAACAGTGGT 
TATTATCAGT GCTACATGAC CGAAGGCAAC AAGGAGGCCG CACACACGCT GTACCTGAAA 
GCAAGAGTGT GTCAGAACTG CATGGAGGTG AATCTGATGG AGGTGGCCAC AATCATCGTG 
GTTGACATCT GTGTCACTCT GGGCTTGCTG CTGCTGGTGT ATTACTGGAG CAAGAGCCGA 
AAGGCCAAAG CCTCGCCTAT GACGAGAGGA GCGGGTGCTG GCGGTAGGCC TCGGGGACAA 
AACAAGGGGA GGCCACCCCC TGTACCCAAT CCAGACTATG AGCCCATCCG GAAAGGCCAG 
CGGGACCTGT ATGCTGGCCT GAATCAGAGA GGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776436.1
CDS	108..686
Translation	MQSGNLWRALGLCLLLVGAWAQDADEQKPYEVSISGNTVELTCPREFEGEIHWKQNDEQMKGYTGKQLLLENFSEMDNSGYYQCYMTEGNKEAAHTLYLKARVCQNCMEVNLMEVATIIVVDICVTLGLLLLVYYWSKSRKAKASPMTRGAGAGGRPRGQNKGRPPPVPNPDYEPIRKGQRDLYAGLNQRGV

Target ORF information:

RefSeq Version	NM_174011.3
Organism	Bos taurus(cattle)
Definition	Bos taurus CD3e molecule (CD3E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174011.3

ORF Insert Sequence:

ATGCAGTCGG GGAATCTCTG GCGAGCTCTG GGACTCTGCC TCTTATTAGT TGGTGCTTGG 
GCACAAGATG CTGATGAACA GAAACCATAT GAAGTCTCCA TCTCTGGAAA CACTGTTGAG 
CTGACATGCC CTAGAGAGTT TGAAGGTGAA ATACATTGGA AACAAAATGA TGAACAAATG 
AAAGGCTATA CTGGGAAACA GCTGTTACTG GAAAACTTTT CAGAAATGGA TAACAGTGGT 
TATTATCAGT GCTACATGAC CGAAGGCAAC AAGGAGGCCG CACACACGCT GTACCTGAAA 
GCAAGAGTGT GTCAGAACTG CATGGAGGTG AATCTGATGG AGGTGGCCAC AATCATCGTG 
GTTGACATCT GTGTCACTCT GGGCTTGCTG CTGCTGGTGT ATTACTGGAG CAAGAGCCGA 
AAGGCCAAAG CCTCGCCTAT GACGAGAGGA GCGGGTGCTG GCGGTAGGCC TCGGGGACAA 
AACAAGGGGA GGCCACCCCC TGTACCCAAT CCAGACTATG AGCCCATCCG GAAAGGCCAG 
CGGGACCTGT ATGCTGGCCT GAATCAGAGA GGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CD3E ( XM_024974979.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024830747.1 Bos taurus CD3e molecule (CD3E), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26203
Clone ID Related Accession (Same CDS sequence)	XM_024974979.1 , NM_174011.3
Accession Version	XM_024974979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	T-cell surface glycoprotein CD3 epsilon chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCGG GGAATCTCTG GCGAGCTCTG GGACTCTGCC TCTTATTAGT TGGTGCTTGG 
GCACAAGATG CTGATGAACA GAAACCATAT GAAGTCTCCA TCTCTGGAAA CACTGTTGAG 
CTGACATGCC CTAGAGAGTT TGAAGGTGAA ATACATTGGA AACAAAATGA TGAACAAATG 
AAAGGCTATA CTGGGAAACA GCTGTTACTG GAAAACTTTT CAGAAATGGA TAACAGTGGT 
TATTATCAGT GCTACATGAC CGAAGGCAAC AAGGAGGCCG CACACACGCT GTACCTGAAA 
GCAAGAGTGT GTCAGAACTG CATGGAGGTG AATCTGATGG AGGTGGCCAC AATCATCGTG 
GTTGACATCT GTGTCACTCT GGGCTTGCTG CTGCTGGTGT ATTACTGGAG CAAGAGCCGA 
AAGGCCAAAG CCTCGCCTAT GACGAGAGGA GCGGGTGCTG GCGGTAGGCC TCGGGGACAA 
AACAAGGGGA GGCCACCCCC TGTACCCAAT CCAGACTATG AGCCCATCCG GAAAGGCCAG 
CGGGACCTGT ATGCTGGCCT GAATCAGAGA GGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024830747.1
CDS	130..708
Translation	MQSGNLWRALGLCLLLVGAWAQDADEQKPYEVSISGNTVELTCPREFEGEIHWKQNDEQMKGYTGKQLLLENFSEMDNSGYYQCYMTEGNKEAAHTLYLKARVCQNCMEVNLMEVATIIVVDICVTLGLLLLVYYWSKSRKAKASPMTRGAGAGGRPRGQNKGRPPPVPNPDYEPIRKGQRDLYAGLNQRGV

Target ORF information:

RefSeq Version	XM_024974979.1
Organism	Bos taurus(cattle)
Definition	Bos taurus CD3e molecule (CD3E), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024974979.1

ORF Insert Sequence:

ATGCAGTCGG GGAATCTCTG GCGAGCTCTG GGACTCTGCC TCTTATTAGT TGGTGCTTGG 
GCACAAGATG CTGATGAACA GAAACCATAT GAAGTCTCCA TCTCTGGAAA CACTGTTGAG 
CTGACATGCC CTAGAGAGTT TGAAGGTGAA ATACATTGGA AACAAAATGA TGAACAAATG 
AAAGGCTATA CTGGGAAACA GCTGTTACTG GAAAACTTTT CAGAAATGGA TAACAGTGGT 
TATTATCAGT GCTACATGAC CGAAGGCAAC AAGGAGGCCG CACACACGCT GTACCTGAAA 
GCAAGAGTGT GTCAGAACTG CATGGAGGTG AATCTGATGG AGGTGGCCAC AATCATCGTG 
GTTGACATCT GTGTCACTCT GGGCTTGCTG CTGCTGGTGT ATTACTGGAG CAAGAGCCGA 
AAGGCCAAAG CCTCGCCTAT GACGAGAGGA GCGGGTGCTG GCGGTAGGCC TCGGGGACAA 
AACAAGGGGA GGCCACCCCC TGTACCCAAT CCAGACTATG AGCCCATCCG GAAAGGCCAG 
CGGGACCTGT ATGCTGGCCT GAATCAGAGA GGCGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cloning, sequencing and expression of the bovine CD3 epsilon and TCR-zeta chains, two invariant components of the T-cell receptor complex.
Gene169(2)165-71(1996 Mar)
Hagens G,Galley Y,Glaser I,Davis WC,Baldwin CL,Clevers H,Dobbelaere DA

Identification of a bovine surface antigen uniquely expressed on CD4-CD8- T cell receptor gamma/delta+ T lymphocytes.
European journal of immunology20(4)809-17(1990 Apr)
Clevers H,MacHugh ND,Bensaid A,Dunlap S,Baldwin CL,Kaushal A,Iams K,Howard CJ,Morrison WI

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CD3E cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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