NPFF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NPFF
Entrez Gene ID	8620
Full Name	neuropeptide FF-amide peptide precursor
Synonyms	FMRFAL
General protein information	Preferred Names neuropeptide FF-amide peptide precursor Names pro-FMRFamide-related neuropeptide FF
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13
Summary	This gene encodes a member of the FMRFamide related peptide (FARP) family of neuropeptides. The encoded preproprotein is proteolytically processed to generate multiple amidated peptides. These peptides may play a role in the regulation of heart rate and blood pressure and the modulation of morphine-induced antinociception. Patients with hypertension exhibit decreased expression of the encoded protein. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016].
Disorder MIM:	604643

mRNA and Protein(s)

mRNA	Protein	Name
NM_001320296.2	NP_001307225.1	pro-FMRFamide-related neuropeptide FF isoform 2
NM_003717.3	NP_003708.1	pro-FMRFamide-related neuropeptide FF isoform 1 preproprotein
XM_024449248.1	XP_024305016.1	pro-FMRFamide-related neuropeptide FF isoform X1
NM_003717.4	NP_003708.1	pro-FMRFamide-related neuropeptide FF isoform 1 preproprotein
NM_001320296.1	NP_001307225.1	pro-FMRFamide-related neuropeptide FF isoform 2

Pathways from BioSystems

Homologies

	NPFF	XP_001106733.1
Mus musculus (house mouse)	Npff	NP_061257.1
Pan troglodytes (chimpanzee)	NPFF	XP_001138078.1
Rattus norvegicus (Norway rat)	Npff	NP_072108.1
Homo sapiens (human)	NPFF	NP_003708.1
Bos taurus (cattle)	NPFF	NP_776548.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene-based pleiotropy across migraine with aura and migraine without aura patient groups.
Zhao H, Eising E, de Vries B, Vijfhuizen LS, , Anttila V, Winsvold BS, Kurth T, Stefansson H, Kallela M, Malik R, Stam AH, Ikram MA, Ligthart L, Freilinger T, Alexander M, M?ller-Myhsok B, Schreiber S, Meitinger T, Aromas A, Eriksson JG, Boomsma DI, van Duijn CM, Zwart JA, Quaye L, Kubisch C, Dichgans M, Wessman M, Stefansson K, Chasman DI, Palotie A, Martin NG, Montgomery GW, Ferrari MD, Terwindt GM, van den Maagdenberg AM, Nyholt DR
Cephalalgia : an international journal of headache36(7)648-57(2016 Jun)

The hypothalamic neuropeptide FF network is impaired in hypertensive patients.
Goncharuk VD, Buijs RM, Jhamandas JH, Swaab DF
Brain and behavior4(4)453-67(2014 Jul)

RFamide Peptides in Early Vertebrate Development.
Sandvik GK, Hodne K, Haug TM, Okubo K, Weltzien FA
Frontiers in endocrinology5203(2014)

Vasopressin (VP) and neuropeptide FF (NPFF) systems in the normal and hypertensive human brainstem.
Goncharuk VD, Buijs RM, Jhamandas JH, Swaab DF
The Journal of comparative neurology519(1)93-124(2011 Jan)

Modulatory role of neuropeptide FF system in nociception and opiate analgesia.
Yang HY, Tao T, Iadarola MJ
Neuropeptides42(1)1-18(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results...highlight the neuropeptide FF-amide peptide encoding gene (NPFF) as a novel candidate risk gene for both migraine with aura and migraine without aura
Title: Gene-based pleiotropy across migraine with aura and migraine without aura patient groups.

reduction of NPFF release within the dorsal motor nucleus and ambiguus nucleus could serve as a possible cause of the impairment of cardiac vagal control in hypertensive patients
Title: Vasopressin (VP) and neuropeptide FF (NPFF) systems in the normal and hypertensive human brainstem.

the findings from the early studies on the analgesic and morphine modulating activities suggested a role for NPFF in pain modulation--review
Title: Modulatory role of neuropeptide FF system in nociception and opiate analgesia.

neuropeptide FF and micro-opioid receptor association could be a possible molecular basis for anti-opioid activity
Title: Physical association between neuropeptide FF and micro-opioid receptors as a possible molecular basis for anti-opioid activity.

expression of hNPFF2 receptor mRNA was up-regulated by neuropeptide FF
Title: Regulation of endogenous human NPFF2 receptor by neuropeptide FF in SK-N-MC neuroblastoma cell line.

The following NPFF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NPFF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu101739	NM_001320296.2 Latest version!	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu31553	NM_003717.4 Latest version!	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu103794	XM_024449248.1 Latest version!	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu31553	NM_003717.3	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu101739	NM_001320296.1	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NPFF ( NM_001320296.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307225.1 Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101739
Clone ID Related Accession (Same CDS sequence)	NM_001320296.2 , NM_001320296.1
Accession Version	NM_001320296.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	pro-FMRFamide-related neuropeptide FF isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC104234.1 and BC035758.1. On Jun 2, 2019 this sequence version replaced NM_001320296.1. Transcript Variant: This variant (2) retains an intron compared to variant 1. The encoded isoform (2) is longer, has a distinct N-terminus, and lacks a predicted signal peptide compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035758.1, BU073391.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTTCCGC AGCCTCCTAC CACTTGCCCC TGGAAGCCAG TCCCTTCCCC TTGTGACTTA 
CGTGTCCAGG GTATTTGCCC ATCTTCCTTC CCTGATACCC CCTTGGCACA GGAGGAAGAC 
AGCGAACCCC TCCCACCACA GGATGCCCAG ACCTCTGGGT CACTGTTGCA CTACCTGCTC 
CAGGCAATGG AGAGACCTGG CCGGAGCCAA GCCTTCCTGT TTCAGCCCCA GAGGTTTGGC 
AGAAATACCC AGGGATCCTG GAGGAATGAA TGGCTGAGTC CCCGGGCTGG AGAGGGGCTG 
AATTCCCAGT TCTGGAGCCT GGCTGCCCCT CAACGCTTTG GGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307225.1
CDS	232..582
Translation	MVPQPPTTCPWKPVPSPCDLRVQGICPSSFPDTPLAQEEDSEPLPPQDAQTSGSLLHYLLQAMERPGRSQAFLFQPQRFGRNTQGSWRNEWLSPRAGEGLNSQFWSLAAPQRFGKK

Target ORF information:

RefSeq Version	NM_001320296.2
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320296.2

ORF Insert Sequence:

ATGGTTCCGC AGCCTCCTAC CACTTGCCCC TGGAAGCCAG TCCCTTCCCC TTGTGACTTA 
CGTGTCCAGG GTATTTGCCC ATCTTCCTTC CCTGATACCC CCTTGGCACA GGAGGAAGAC 
AGCGAACCCC TCCCACCACA GGATGCCCAG ACCTCTGGGT CACTGTTGCA CTACCTGCTC 
CAGGCAATGG AGAGACCTGG CCGGAGCCAA GCCTTCCTGT TTCAGCCCCA GAGGTTTGGC 
AGAAATACCC AGGGATCCTG GAGGAATGAA TGGCTGAGTC CCCGGGCTGG AGAGGGGCTG 
AATTCCCAGT TCTGGAGCCT GGCTGCCCCT CAACGCTTTG GGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPFF ( NM_001320296.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307225.1 Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101739
Clone ID Related Accession (Same CDS sequence)	NM_001320296.2 , NM_001320296.1
Accession Version	NM_001320296.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	pro-FMRFamide-related neuropeptide FF isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF005271.1, BM314163.1, BC104234.1 and BC035758.1. Transcript Variant: This variant (2) retains an intron compared to variant 1. The encoded isoform (2) is longer, has a distinct N-terminus, and lacks a predicted signal peptide compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035758.1, ERR279841.4689.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTCCGC AGCCTCCTAC CACTTGCCCC TGGAAGCCAG TCCCTTCCCC TTGTGACTTA 
CGTGTCCAGG GTATTTGCCC ATCTTCCTTC CCTGATACCC CCTTGGCACA GGAGGAAGAC 
AGCGAACCCC TCCCACCACA GGATGCCCAG ACCTCTGGGT CACTGTTGCA CTACCTGCTC 
CAGGCAATGG AGAGACCTGG CCGGAGCCAA GCCTTCCTGT TTCAGCCCCA GAGGTTTGGC 
AGAAATACCC AGGGATCCTG GAGGAATGAA TGGCTGAGTC CCCGGGCTGG AGAGGGGCTG 
AATTCCCAGT TCTGGAGCCT GGCTGCCCCT CAACGCTTTG GGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307225.1
CDS	386..736
Translation	MVPQPPTTCPWKPVPSPCDLRVQGICPSSFPDTPLAQEEDSEPLPPQDAQTSGSLLHYLLQAMERPGRSQAFLFQPQRFGRNTQGSWRNEWLSPRAGEGLNSQFWSLAAPQRFGKK

Target ORF information:

RefSeq Version	NM_001320296.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320296.1

ORF Insert Sequence:

ATGGTTCCGC AGCCTCCTAC CACTTGCCCC TGGAAGCCAG TCCCTTCCCC TTGTGACTTA 
CGTGTCCAGG GTATTTGCCC ATCTTCCTTC CCTGATACCC CCTTGGCACA GGAGGAAGAC 
AGCGAACCCC TCCCACCACA GGATGCCCAG ACCTCTGGGT CACTGTTGCA CTACCTGCTC 
CAGGCAATGG AGAGACCTGG CCGGAGCCAA GCCTTCCTGT TTCAGCCCCA GAGGTTTGGC 
AGAAATACCC AGGGATCCTG GAGGAATGAA TGGCTGAGTC CCCGGGCTGG AGAGGGGCTG 
AATTCCCAGT TCTGGAGCCT GGCTGCCCCT CAACGCTTTG GGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPFF ( NM_003717.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003708.1 Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31553
Clone ID Related Accession (Same CDS sequence)	NM_003717.3 , NM_003717.4
Accession Version	NM_003717.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	pro-FMRFamide-related neuropeptide FF isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF005271.1, BM314163.1 and BC035758.1. On Feb 19, 2016 this sequence version replaced NM_003717.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF005271.1, AA335098.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCTA GGCAGGCTGC TGCACTGCTG GTGCTGCTGC TGTTAATAGA CGGGGGCTGT 
GCTGAAGGGC CAGGAGGCCA GCAGGAAGAC CAGCTCTCCG CGGAGGAAGA CAGCGAACCC 
CTCCCACCAC AGGATGCCCA GACCTCTGGG TCACTGTTGC ACTACCTGCT CCAGGCAATG 
GAGAGACCTG GCCGGAGCCA AGCCTTCCTG TTTCAGCCCC AGAGGTTTGG CAGAAATACC 
CAGGGATCCT GGAGGAATGA ATGGCTGAGT CCCCGGGCTG GAGAGGGGCT GAATTCCCAG 
TTCTGGAGCC TGGCTGCCCC TCAACGCTTT GGGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003708.1
CDS	165..506
Translation	MDSRQAAALLVLLLLIDGGCAEGPGGQQEDQLSAEEDSEPLPPQDAQTSGSLLHYLLQAMERPGRSQAFLFQPQRFGRNTQGSWRNEWLSPRAGEGLNSQFWSLAAPQRFGKK

Target ORF information:

RefSeq Version	NM_003717.3
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003717.3

ORF Insert Sequence:

ATGGATTCTA GGCAGGCTGC TGCACTGCTG GTGCTGCTGC TGTTAATAGA CGGGGGCTGT 
GCTGAAGGGC CAGGAGGCCA GCAGGAAGAC CAGCTCTCCG CGGAGGAAGA CAGCGAACCC 
CTCCCACCAC AGGATGCCCA GACCTCTGGG TCACTGTTGC ACTACCTGCT CCAGGCAATG 
GAGAGACCTG GCCGGAGCCA AGCCTTCCTG TTTCAGCCCC AGAGGTTTGG CAGAAATACC 
CAGGGATCCT GGAGGAATGA ATGGCTGAGT CCCCGGGCTG GAGAGGGGCT GAATTCCCAG 
TTCTGGAGCC TGGCTGCCCC TCAACGCTTT GGGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPFF ( NM_003717.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003708.1 Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31553
Clone ID Related Accession (Same CDS sequence)	NM_003717.3 , NM_003717.4
Accession Version	NM_003717.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 342bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	pro-FMRFamide-related neuropeptide FF isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF005271.1 and BC035758.1. On Jun 2, 2019 this sequence version replaced NM_003717.3. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF005271.1, AA336101.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCTA GGCAGGCTGC TGCACTGCTG GTGCTGCTGC TGTTAATAGA CGGGGGCTGT 
GCTGAAGGGC CAGGAGGCCA GCAGGAAGAC CAGCTCTCCG CGGAGGAAGA CAGCGAACCC 
CTCCCACCAC AGGATGCCCA GACCTCTGGG TCACTGTTGC ACTACCTGCT CCAGGCAATG 
GAGAGACCTG GCCGGAGCCA AGCCTTCCTG TTTCAGCCCC AGAGGTTTGG CAGAAATACC 
CAGGGATCCT GGAGGAATGA ATGGCTGAGT CCCCGGGCTG GAGAGGGGCT GAATTCCCAG 
TTCTGGAGCC TGGCTGCCCC TCAACGCTTT GGGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003708.1
CDS	11..352
Translation	MDSRQAAALLVLLLLIDGGCAEGPGGQQEDQLSAEEDSEPLPPQDAQTSGSLLHYLLQAMERPGRSQAFLFQPQRFGRNTQGSWRNEWLSPRAGEGLNSQFWSLAAPQRFGKK

Target ORF information:

RefSeq Version	NM_003717.4
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003717.4

ORF Insert Sequence:

ATGGATTCTA GGCAGGCTGC TGCACTGCTG GTGCTGCTGC TGTTAATAGA CGGGGGCTGT 
GCTGAAGGGC CAGGAGGCCA GCAGGAAGAC CAGCTCTCCG CGGAGGAAGA CAGCGAACCC 
CTCCCACCAC AGGATGCCCA GACCTCTGGG TCACTGTTGC ACTACCTGCT CCAGGCAATG 
GAGAGACCTG GCCGGAGCCA AGCCTTCCTG TTTCAGCCCC AGAGGTTTGG CAGAAATACC 
CAGGGATCCT GGAGGAATGA ATGGCTGAGT CCCCGGGCTG GAGAGGGGCT GAATTCCCAG 
TTCTGGAGCC TGGCTGCCCC TCAACGCTTT GGGAAGAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NPFF ( XM_024449248.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305016.1 Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu103794
Clone ID Related Accession (Same CDS sequence)	XM_024449248.1
Accession Version	XM_024449248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	pro-FMRFamide-related neuropeptide FF isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTCT TGTGCTTTAG TCTACAGGAG GAAGACAGCG AACCCCTCCC ACCACAGGAT 
GCCCAGACCT CTGGGTCACT GTTGCACTAC CTGCTCCAGG CAATGGAGAG ACCTGGCCGG 
AGCCAAGCCT TCCTGTTTCA GCCCCAGAGG TTTGGCAGAA ATACCCAGGG ATCCTGGAGG 
AATGAATGGC TGAGTCCCCG GGCTGGAGAG GGGCTGAATT CCCAGTTCTG GAGCCTGGCT 
GCCCCTCAAC GCTTTGGGAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305016.1
CDS	54..320
Translation	MSFLCFSLQEEDSEPLPPQDAQTSGSLLHYLLQAMERPGRSQAFLFQPQRFGRNTQGSWRNEWLSPRAGEGLNSQFWSLAAPQRFGKK

Target ORF information:

RefSeq Version	XM_024449248.1
Organism	Homo sapiens(human)
Definition	Homo sapiens neuropeptide FF-amide peptide precursor (NPFF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449248.1

ORF Insert Sequence:

ATGAGTTTCT TGTGCTTTAG TCTACAGGAG GAAGACAGCG AACCCCTCCC ACCACAGGAT 
GCCCAGACCT CTGGGTCACT GTTGCACTAC CTGCTCCAGG CAATGGAGAG ACCTGGCCGG 
AGCCAAGCCT TCCTGTTTCA GCCCCAGAGG TTTGGCAGAA ATACCCAGGG ATCCTGGAGG 
AATGAATGGC TGAGTCCCCG GGCTGGAGAG GGGCTGAATT CCCAGTTCTG GAGCCTGGCT 
GCCCCTCAAC GCTTTGGGAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene-based pleiotropy across migraine with aura and migraine without aura patient groups.
Cephalalgia : an international journal of headache36(7)648-57(2016 Jun)
Zhao H,Eising E,de Vries B,Vijfhuizen LS,,Anttila V,Winsvold BS,Kurth T,Stefansson H,Kallela M,Malik R,Stam AH,Ikram MA,Ligthart L,Freilinger T,Alexander M,M?ller-Myhsok B,Schreiber S,Meitinger T,Aromas A,Eriksson JG,Boomsma DI,van Duijn CM,Zwart JA,Quaye L,Kubisch C,Dichgans M,Wessman M,Stefansson K,Chasman DI,Palotie A,Martin NG,Montgomery GW,Ferrari MD,Terwindt GM,van den Maagdenberg AM,Nyholt DR

The hypothalamic neuropeptide FF network is impaired in hypertensive patients.
Brain and behavior4(4)453-67(2014 Jul)
Goncharuk VD,Buijs RM,Jhamandas JH,Swaab DF

RFamide Peptides in Early Vertebrate Development.
Frontiers in endocrinology5203(2014)
Sandvik GK,Hodne K,Haug TM,Okubo K,Weltzien FA

Vasopressin (VP) and neuropeptide FF (NPFF) systems in the normal and hypertensive human brainstem.
The Journal of comparative neurology519(1)93-124(2011 Jan)
Goncharuk VD,Buijs RM,Jhamandas JH,Swaab DF

Modulatory role of neuropeptide FF system in nociception and opiate analgesia.
Neuropeptides42(1)1-18(2008 Feb)
Yang HY,Tao T,Iadarola MJ

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NPFF cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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