DEGS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DEGS1
Entrez Gene ID	8560
Full Name	delta 4-desaturase, sphingolipid 1
Synonyms	DEGS,DEGS-1,DES1,Des-1,FADS7,MIG15,MLD
General protein information	Preferred Names delta 4-desaturase, sphingolipid 1 Names sphingolipid delta(4)-desaturase DES1 cell migration-inducing gene 15 protein degenerative spermatocyte homolog 1, lipid desaturase degenerative spermatocyte homolog, lipid desaturase dihydroceramide desaturase 1 membrane fatty acid (lipid) desaturase membrane lipid desaturase migration-inducing gene 15 protein sphingolipid delta 4 desaturase sphingolipid delta(4)-desaturase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.11
Summary	This gene encodes a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. This protein contains three His-containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It is predicted to be a multiple membrane-spanning protein localized to the endoplasmic reticulum. Overexpression of this gene inhibited biosynthesis of the EGF receptor, suggesting a possible role of a fatty acid desaturase in regulating biosynthetic processing of the EGF receptor. [provided by RefSeq, Mar 2010].
Disorder MIM:	615843

mRNA and Protein(s)

mRNA	Protein	Name
NM_001321541.1	NP_001308470.1	sphingolipid delta(4)-desaturase DES1 isoform 2
XM_017002648.2	XP_016858137.1	sphingolipid delta(4)-desaturase DES1 isoform X1
NM_001321542.1	NP_001308471.1	sphingolipid delta(4)-desaturase DES1 isoform 3
NM_003676.3	NP_003667.1	sphingolipid delta(4)-desaturase DES1 isoform 1
XM_017002648.1	XP_016858137.1	sphingolipid delta(4)-desaturase DES1 isoform X1
NM_001321541.2	NP_001308470.1	sphingolipid delta(4)-desaturase DES1 isoform 2
NM_001321542.2	NP_001308471.1	sphingolipid delta(4)-desaturase DES1 isoform 3
NM_003676.4	NP_003667.1	sphingolipid delta(4)-desaturase DES1 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DEGS1	NP_001029461.1
Rattus norvegicus (Norway rat)	Degs1	NP_445775.2
Gallus gallus (chicken)	DEGS1	NP_001012583.1
Pan troglodytes (chimpanzee)	DEGS1	XP_514228.3
	DEGS1	XP_001096845.1
Xenopus tropicalis (tropical clawed frog)	degs1	NP_001007485.1
Homo sapiens (human)	DEGS1	NP_003667.1
Canis lupus familiaris (dog)	DEGS1	XP_005623032.1
Mus musculus (house mouse)	Degs1	NP_031879.1
Danio rerio (zebrafish)	degs1	NP_997865.1

Gene Ontology
Bibliography

Related articles in PubMed

Dihydroceramide-desaturase-1-mediated caspase 9 activation through ceramide plays a pivotal role in palmitic acid-induced HepG2 cell apoptosis.
Zhu Q, Yang J, Zhu R, Jiang X, Li W, He S, Jin J
Apoptosis : an international journal on programmed cell death21(9)1033-44(2016 Sep)

The Sphingosine Kinase 2 Inhibitor ABC294640 Reduces the Growth of Prostate Cancer Cells and Results in Accumulation of Dihydroceramides In Vitro and In Vivo.
Venant H, Rahmaniyan M, Jones EE, Lu P, Lilly MB, Garrett-Mayer E, Drake RR, Kraveka JM, Smith CD, Voelkel-Johnson C
Molecular cancer therapeutics14(12)2744-52(2015 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ABC294640 may reduce the proliferative capacity of castration-resistant prostate cancer cells through inhibition of both sphingosine kinase 2 and dihydroceramide desaturase.
Title: The Sphingosine Kinase 2 Inhibitor ABC294640 Reduces the Growth of Prostate Cancer Cells and Results in Accumulation of Dihydroceramides In Vitro and In Vivo.

SKI II is a noncompetitive inhibitor (Ki = 0.3 muM) of Des1 activity with effect also in intact cells without modifying Des1 protein levels. Molecular modeling studies support that the SKI II-induced decrease in Des1 activity could result from inhibition of NADH-cytochrome b5 reductase.
Title: Inhibition of dihydroceramide desaturase activity by the sphingosine kinase inhibitor SKI II.

Dihydroceramide desaturase 1 (DES) is a potential molecular target for regulating apoptotic resistance to photodynamic therapy
Title: Dihydroceramide desaturase knockdown impacts sphingolipids and apoptosis after photodamage in human head and neck squamous carcinoma cells.

changes in lipid homeostasis and gene expression in Huh7 hepatocytes when the synthesis of ceramide is perturbed by knocking down serine pal mitoyltransferase subunits 1, 2, and 3 (SPTLC123) or dihydroceramide desaturase 1 (DEGS1)
Title: Silencing of enzymes involved in ceramide biosynthesis causes distinct global alterations of lipid homeostasis and gene expression.

a role of the sphingolipid pathway, dihydroceramides desaturase in particular, in confluence-induced growth arrest in neuroblastoma cells
Title: Cell density-dependent reduction of dihydroceramide desaturase activity in neuroblastoma cells.

The following DEGS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEGS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27921	NM_003676.4 Latest version!	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu35153	NM_001321541.2 Latest version!	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu61983	NM_001321542.2 Latest version!	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu61983	XM_017002648.2 Latest version!	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu61983	XM_017002648.1	Homo sapiens delta(4)-desaturase, sphingolipid 1 (DEGS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu35153	NM_001321541.1	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu61983	NM_001321542.1	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu27921	NM_003676.3	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DEGS1 ( NM_003676.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003667.1 Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27921
Clone ID Related Accession (Same CDS sequence)	NM_003676.3 , NM_003676.4
Accession Version	NM_003676.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	sphingolipid delta(4)-desaturase DES1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU126996.1, BC000961.2 and AY423730.1. On Dec 13, 2011 this sequence version replaced NM_003676.2. ##Evidence-Data-START## Transcript exon combination :: BC000961.2, SRR1803614.236843.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGCC GCGTCTCGCG GGAAGACTTC GAGTGGGTCT ACACCGACCA GCCGCACGCC 
GACCGGCGCC GGGAGATCCT GGCAAAGTAT CCAGAGATAA AGTCCTTGAT GAAACCTGAT 
CCCAATTTGA TATGGATTAT AATTATGATG GTTCTCACCC AGTTGGGTGC ATTTTACATA 
GTAAAAGACT TGGACTGGAA ATGGGTCATA TTTGGGGCCT ATGCGTTTGG CAGTTGCATT 
AACCACTCAA TGACTCTGGC TATTCATGAG ATTGCCCACA ATGCTGCCTT TGGCAACTGC 
AAAGCAATGT GGAATCGCTG GTTTGGAATG TTTGCTAATC TTCCTATTGG GATTCCATAT 
TCAATTTCCT TTAAGAGGTA TCACATGGAT CATCATCGGT ACCTTGGAGC TGATGGCGTC 
GATGTAGATA TTCCTACCGA TTTTGAGGGC TGGTTCTTCT GTACCGCTTT CAGAAAGTTT 
ATATGGGTTA TTCTTCAGCC TCTCTTTTAT GCCTTTCGAC CTCTGTTCAT CAACCCCAAA 
CCAATTACGT ATCTGGAAGT TATCAATACC GTGGCACAGG TCACTTTTGA CATTTTAATT 
TATTACTTTT TGGGAATTAA ATCCTTAGTC TACATGTTGG CAGCATCTTT ACTTGGCCTG 
GGTTTGCACC CAATTTCTGG ACATTTTATA GCTGAGCATT ACATGTTCTT AAAGGGTCAT 
GAAACTTACT CATATTATGG GCCTCTGAAT TTACTTACCT TCAATGTGGG TTATCATAAT 
GAACATCATG ATTTCCCCAA CATTCCTGGA AAAAGTCTTC CACTGGTGAG GAAAATAGCA 
GCTGAATACT ATGACAACCT CCCTCACTAC AATTCCTGGA TAAAAGTACT GTATGATTTT 
GTGATGGATG ATACAATAAG TCCCTACTCA AGAATGAAGA GGCACCAAAA AGGAGAGATG 
GTGCTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003667.1
CDS	130..1101
Translation	MGSRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQKGEMVLE

Target ORF information:

RefSeq Version	NM_003676.3
Organism	Homo sapiens(human)
Definition	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003676.3

ORF Insert Sequence:

ATGGGGAGCC GCGTCTCGCG GGAAGACTTC GAGTGGGTCT ACACCGACCA GCCGCACGCC 
GACCGGCGCC GGGAGATCCT GGCAAAGTAT CCAGAGATAA AGTCCTTGAT GAAACCTGAT 
CCCAATTTGA TATGGATTAT AATTATGATG GTTCTCACCC AGTTGGGTGC ATTTTACATA 
GTAAAAGACT TGGACTGGAA ATGGGTCATA TTTGGGGCCT ATGCGTTTGG CAGTTGCATT 
AACCACTCAA TGACTCTGGC TATTCATGAG ATTGCCCACA ATGCTGCCTT TGGCAACTGC 
AAAGCAATGT GGAATCGCTG GTTTGGAATG TTTGCTAATC TTCCTATTGG GATTCCATAT 
TCAATTTCCT TTAAGAGGTA TCACATGGAT CATCATCGGT ACCTTGGAGC TGATGGCGTC 
GATGTAGATA TTCCTACCGA TTTTGAGGGC TGGTTCTTCT GTACCGCTTT CAGAAAGTTT 
ATATGGGTTA TTCTTCAGCC TCTCTTTTAT GCCTTTCGAC CTCTGTTCAT CAACCCCAAA 
CCAATTACGT ATCTGGAAGT TATCAATACC GTGGCACAGG TCACTTTTGA CATTTTAATT 
TATTACTTTT TGGGAATTAA ATCCTTAGTC TACATGTTGG CAGCATCTTT ACTTGGCCTG 
GGTTTGCACC CAATTTCTGG ACATTTTATA GCTGAGCATT ACATGTTCTT AAAGGGTCAT 
GAAACTTACT CATATTATGG GCCTCTGAAT TTACTTACCT TCAATGTGGG TTATCATAAT 
GAACATCATG ATTTCCCCAA CATTCCTGGA AAAAGTCTTC CACTGGTGAG GAAAATAGCA 
GCTGAATACT ATGACAACCT CCCTCACTAC AATTCCTGGA TAAAAGTACT GTATGATTTT 
GTGATGGATG ATACAATAAG TCCCTACTCA AGAATGAAGA GGCACCAAAA AGGAGAGATG 
GTGCTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEGS1 ( NM_003676.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003667.1 Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27921
Clone ID Related Accession (Same CDS sequence)	NM_003676.3 , NM_003676.4
Accession Version	NM_003676.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	sphingolipid delta(4)-desaturase DES1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC000961.2 and AY423730.1. On Nov 22, 2018 this sequence version replaced NM_003676.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000961.2, SRR3476690.245725.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000323699.9/ ENSP00000316476.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAGCC GCGTCTCGCG GGAAGACTTC GAGTGGGTCT ACACCGACCA GCCGCACGCC 
GACCGGCGCC GGGAGATCCT GGCAAAGTAT CCAGAGATAA AGTCCTTGAT GAAACCTGAT 
CCCAATTTGA TATGGATTAT AATTATGATG GTTCTCACCC AGTTGGGTGC ATTTTACATA 
GTAAAAGACT TGGACTGGAA ATGGGTCATA TTTGGGGCCT ATGCGTTTGG CAGTTGCATT 
AACCACTCAA TGACTCTGGC TATTCATGAG ATTGCCCACA ATGCTGCCTT TGGCAACTGC 
AAAGCAATGT GGAATCGCTG GTTTGGAATG TTTGCTAATC TTCCTATTGG GATTCCATAT 
TCAATTTCCT TTAAGAGGTA TCACATGGAT CATCATCGGT ACCTTGGAGC TGATGGCGTC 
GATGTAGATA TTCCTACCGA TTTTGAGGGC TGGTTCTTCT GTACCGCTTT CAGAAAGTTT 
ATATGGGTTA TTCTTCAGCC TCTCTTTTAT GCCTTTCGAC CTCTGTTCAT CAACCCCAAA 
CCAATTACGT ATCTGGAAGT TATCAATACC GTGGCACAGG TCACTTTTGA CATTTTAATT 
TATTACTTTT TGGGAATTAA ATCCTTAGTC TACATGTTGG CAGCATCTTT ACTTGGCCTG 
GGTTTGCACC CAATTTCTGG ACATTTTATA GCTGAGCATT ACATGTTCTT AAAGGGTCAT 
GAAACTTACT CATATTATGG GCCTCTGAAT TTACTTACCT TCAATGTGGG TTATCATAAT 
GAACATCATG ATTTCCCCAA CATTCCTGGA AAAAGTCTTC CACTGGTGAG GAAAATAGCA 
GCTGAATACT ATGACAACCT CCCTCACTAC AATTCCTGGA TAAAAGTACT GTATGATTTT 
GTGATGGATG ATACAATAAG TCCCTACTCA AGAATGAAGA GGCACCAAAA AGGAGAGATG 
GTGCTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003667.1
CDS	98..1069
Translation	MGSRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQKGEMVLE

Target ORF information:

RefSeq Version	NM_003676.4
Organism	Homo sapiens(human)
Definition	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003676.4

ORF Insert Sequence:

ATGGGGAGCC GCGTCTCGCG GGAAGACTTC GAGTGGGTCT ACACCGACCA GCCGCACGCC 
GACCGGCGCC GGGAGATCCT GGCAAAGTAT CCAGAGATAA AGTCCTTGAT GAAACCTGAT 
CCCAATTTGA TATGGATTAT AATTATGATG GTTCTCACCC AGTTGGGTGC ATTTTACATA 
GTAAAAGACT TGGACTGGAA ATGGGTCATA TTTGGGGCCT ATGCGTTTGG CAGTTGCATT 
AACCACTCAA TGACTCTGGC TATTCATGAG ATTGCCCACA ATGCTGCCTT TGGCAACTGC 
AAAGCAATGT GGAATCGCTG GTTTGGAATG TTTGCTAATC TTCCTATTGG GATTCCATAT 
TCAATTTCCT TTAAGAGGTA TCACATGGAT CATCATCGGT ACCTTGGAGC TGATGGCGTC 
GATGTAGATA TTCCTACCGA TTTTGAGGGC TGGTTCTTCT GTACCGCTTT CAGAAAGTTT 
ATATGGGTTA TTCTTCAGCC TCTCTTTTAT GCCTTTCGAC CTCTGTTCAT CAACCCCAAA 
CCAATTACGT ATCTGGAAGT TATCAATACC GTGGCACAGG TCACTTTTGA CATTTTAATT 
TATTACTTTT TGGGAATTAA ATCCTTAGTC TACATGTTGG CAGCATCTTT ACTTGGCCTG 
GGTTTGCACC CAATTTCTGG ACATTTTATA GCTGAGCATT ACATGTTCTT AAAGGGTCAT 
GAAACTTACT CATATTATGG GCCTCTGAAT TTACTTACCT TCAATGTGGG TTATCATAAT 
GAACATCATG ATTTCCCCAA CATTCCTGGA AAAAGTCTTC CACTGGTGAG GAAAATAGCA 
GCTGAATACT ATGACAACCT CCCTCACTAC AATTCCTGGA TAAAAGTACT GTATGATTTT 
GTGATGGATG ATACAATAAG TCCCTACTCA AGAATGAAGA GGCACCAAAA AGGAGAGATG 
GTGCTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEGS1 ( NM_001321541.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308470.1 Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu35153
Clone ID Related Accession (Same CDS sequence)	NM_001321541.1 , NM_001321541.2
Accession Version	NM_001321541.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	sphingolipid delta(4)-desaturase DES1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092809.2. On Mar 26, 2016 this sequence version replaced XM_011544317.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.228118.1, SRR1803617.126676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGCC GCGTCTCGCG GGAAGACTTC GAGTGGGTCT ACACCGACCA GCCGCACGCC 
GACCGGCGCC GGGAGATCCT GGCAAAGTAT CCAGAGATAA AGTCCTTGAT GAAACCTGAT 
CCCAATTTGA TATGGATTAT AATTATGATG GTTCTCACCC AGTTGGGTGC ATTTTACATA 
GTAAAAGACT TGGACTGGAA ATGGGTCATA TTTGGGGCCT ATGCGTTTGG CAGTTGCATT 
AACCACTCAA TGACTCTGGC TATTCATGAG ATTGCCCACA ATGCTGCCTT TGGCAACTGC 
AAAGCAATGT GGAATCGCTG GTTTGGAATG TTTGCTAATC TTCCTATTGG GATTCCATAT 
TCAATTTCCT TTAAGAGGTA TCACATGGAT CATCATCGGT ACCTTGGAGC TGATGGCGTC 
GATGTAGATA TTCCTACCGA TTTTGAGGGC TGGTTCTTCT GTACCGCTTT CAGAAAGTTT 
ATATGGGTTA TTCTTCAGCC TCTCTTTTAT GCCTTTCGAC CTCTGTTCAT CAACCCCAAA 
CCAATTACGT ATCTGGAAGT TATCAATACC GTGGCACAGG TCACTTTTGA CATTTTAATT 
TATTACTTTT TGGGAATTAA ATCCTTAGTC TACATGTTGG CAGCATCTTT ACTTGGCCTG 
GGTTTGCACC CAATTTCTGG ACATTTTATA GCTGAGCATT ACATGTTCTT AAAGGGTCAT 
GAAACTTACT CATATTATGG GCCTCTGAAT TTACTTACCT TCAATGTGGG TTATCATAAT 
GAACATCATG ATTTCCCCAA CATTCCTGGA AAAAGTCTTC CACTGAGCTG GAGTTTCCTG 
GATGGGTGCA GTGGCTCACA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308470.1
CDS	130..996
Translation	MGSRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLSWSFLDGCSGSHL

Target ORF information:

RefSeq Version	NM_001321541.1
Organism	Homo sapiens(human)
Definition	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321541.1

ORF Insert Sequence:

ATGGGGAGCC GCGTCTCGCG GGAAGACTTC GAGTGGGTCT ACACCGACCA GCCGCACGCC 
GACCGGCGCC GGGAGATCCT GGCAAAGTAT CCAGAGATAA AGTCCTTGAT GAAACCTGAT 
CCCAATTTGA TATGGATTAT AATTATGATG GTTCTCACCC AGTTGGGTGC ATTTTACATA 
GTAAAAGACT TGGACTGGAA ATGGGTCATA TTTGGGGCCT ATGCGTTTGG CAGTTGCATT 
AACCACTCAA TGACTCTGGC TATTCATGAG ATTGCCCACA ATGCTGCCTT TGGCAACTGC 
AAAGCAATGT GGAATCGCTG GTTTGGAATG TTTGCTAATC TTCCTATTGG GATTCCATAT 
TCAATTTCCT TTAAGAGGTA TCACATGGAT CATCATCGGT ACCTTGGAGC TGATGGCGTC 
GATGTAGATA TTCCTACCGA TTTTGAGGGC TGGTTCTTCT GTACCGCTTT CAGAAAGTTT 
ATATGGGTTA TTCTTCAGCC TCTCTTTTAT GCCTTTCGAC CTCTGTTCAT CAACCCCAAA 
CCAATTACGT ATCTGGAAGT TATCAATACC GTGGCACAGG TCACTTTTGA CATTTTAATT 
TATTACTTTT TGGGAATTAA ATCCTTAGTC TACATGTTGG CAGCATCTTT ACTTGGCCTG 
GGTTTGCACC CAATTTCTGG ACATTTTATA GCTGAGCATT ACATGTTCTT AAAGGGTCAT 
GAAACTTACT CATATTATGG GCCTCTGAAT TTACTTACCT TCAATGTGGG TTATCATAAT 
GAACATCATG ATTTCCCCAA CATTCCTGGA AAAAGTCTTC CACTGAGCTG GAGTTTCCTG 
GATGGGTGCA GTGGCTCACA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEGS1 ( NM_001321541.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308470.1 Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu35153
Clone ID Related Accession (Same CDS sequence)	NM_001321541.1 , NM_001321541.2
Accession Version	NM_001321541.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	sphingolipid delta(4)-desaturase DES1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092809.2. On May 31, 2019 this sequence version replaced NM_001321541.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.126676.1, SRR1660803.238931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGAGCC GCGTCTCGCG GGAAGACTTC GAGTGGGTCT ACACCGACCA GCCGCACGCC 
GACCGGCGCC GGGAGATCCT GGCAAAGTAT CCAGAGATAA AGTCCTTGAT GAAACCTGAT 
CCCAATTTGA TATGGATTAT AATTATGATG GTTCTCACCC AGTTGGGTGC ATTTTACATA 
GTAAAAGACT TGGACTGGAA ATGGGTCATA TTTGGGGCCT ATGCGTTTGG CAGTTGCATT 
AACCACTCAA TGACTCTGGC TATTCATGAG ATTGCCCACA ATGCTGCCTT TGGCAACTGC 
AAAGCAATGT GGAATCGCTG GTTTGGAATG TTTGCTAATC TTCCTATTGG GATTCCATAT 
TCAATTTCCT TTAAGAGGTA TCACATGGAT CATCATCGGT ACCTTGGAGC TGATGGCGTC 
GATGTAGATA TTCCTACCGA TTTTGAGGGC TGGTTCTTCT GTACCGCTTT CAGAAAGTTT 
ATATGGGTTA TTCTTCAGCC TCTCTTTTAT GCCTTTCGAC CTCTGTTCAT CAACCCCAAA 
CCAATTACGT ATCTGGAAGT TATCAATACC GTGGCACAGG TCACTTTTGA CATTTTAATT 
TATTACTTTT TGGGAATTAA ATCCTTAGTC TACATGTTGG CAGCATCTTT ACTTGGCCTG 
GGTTTGCACC CAATTTCTGG ACATTTTATA GCTGAGCATT ACATGTTCTT AAAGGGTCAT 
GAAACTTACT CATATTATGG GCCTCTGAAT TTACTTACCT TCAATGTGGG TTATCATAAT 
GAACATCATG ATTTCCCCAA CATTCCTGGA AAAAGTCTTC CACTGAGCTG GAGTTTCCTG 
GATGGGTGCA GTGGCTCACA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308470.1
CDS	98..964
Translation	MGSRVSREDFEWVYTDQPHADRRREILAKYPEIKSLMKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLSWSFLDGCSGSHL

Target ORF information:

RefSeq Version	NM_001321541.2
Organism	Homo sapiens(human)
Definition	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321541.2

ORF Insert Sequence:

ATGGGGAGCC GCGTCTCGCG GGAAGACTTC GAGTGGGTCT ACACCGACCA GCCGCACGCC 
GACCGGCGCC GGGAGATCCT GGCAAAGTAT CCAGAGATAA AGTCCTTGAT GAAACCTGAT 
CCCAATTTGA TATGGATTAT AATTATGATG GTTCTCACCC AGTTGGGTGC ATTTTACATA 
GTAAAAGACT TGGACTGGAA ATGGGTCATA TTTGGGGCCT ATGCGTTTGG CAGTTGCATT 
AACCACTCAA TGACTCTGGC TATTCATGAG ATTGCCCACA ATGCTGCCTT TGGCAACTGC 
AAAGCAATGT GGAATCGCTG GTTTGGAATG TTTGCTAATC TTCCTATTGG GATTCCATAT 
TCAATTTCCT TTAAGAGGTA TCACATGGAT CATCATCGGT ACCTTGGAGC TGATGGCGTC 
GATGTAGATA TTCCTACCGA TTTTGAGGGC TGGTTCTTCT GTACCGCTTT CAGAAAGTTT 
ATATGGGTTA TTCTTCAGCC TCTCTTTTAT GCCTTTCGAC CTCTGTTCAT CAACCCCAAA 
CCAATTACGT ATCTGGAAGT TATCAATACC GTGGCACAGG TCACTTTTGA CATTTTAATT 
TATTACTTTT TGGGAATTAA ATCCTTAGTC TACATGTTGG CAGCATCTTT ACTTGGCCTG 
GGTTTGCACC CAATTTCTGG ACATTTTATA GCTGAGCATT ACATGTTCTT AAAGGGTCAT 
GAAACTTACT CATATTATGG GCCTCTGAAT TTACTTACCT TCAATGTGGG TTATCATAAT 
GAACATCATG ATTTCCCCAA CATTCCTGGA AAAAGTCTTC CACTGAGCTG GAGTTTCCTG 
GATGGGTGCA GTGGCTCACA CCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEGS1 ( NM_001321542.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308471.1 Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu61983
Clone ID Related Accession (Same CDS sequence)	XM_017002648.2 , NM_001321542.1 , XM_017002648.1 , NM_001321542.2
Accession Version	NM_001321542.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	sphingolipid delta(4)-desaturase DES1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092809.2. On Mar 26, 2016 this sequence version replaced XM_011544318.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.114778.1, SRR1660803.192123.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCTG ATCCCAATTT GATATGGATT ATAATTATGA TGGTTCTCAC CCAGTTGGGT 
GCATTTTACA TAGTAAAAGA CTTGGACTGG AAATGGGTCA TATTTGGGGC CTATGCGTTT 
GGCAGTTGCA TTAACCACTC AATGACTCTG GCTATTCATG AGATTGCCCA CAATGCTGCC 
TTTGGCAACT GCAAAGCAAT GTGGAATCGC TGGTTTGGAA TGTTTGCTAA TCTTCCTATT 
GGGATTCCAT ATTCAATTTC CTTTAAGAGG TATCACATGG ATCATCATCG GTACCTTGGA 
GCTGATGGCG TCGATGTAGA TATTCCTACC GATTTTGAGG GCTGGTTCTT CTGTACCGCT 
TTCAGAAAGT TTATATGGGT TATTCTTCAG CCTCTCTTTT ATGCCTTTCG ACCTCTGTTC 
ATCAACCCCA AACCAATTAC GTATCTGGAA GTTATCAATA CCGTGGCACA GGTCACTTTT 
GACATTTTAA TTTATTACTT TTTGGGAATT AAATCCTTAG TCTACATGTT GGCAGCATCT 
TTACTTGGCC TGGGTTTGCA CCCAATTTCT GGACATTTTA TAGCTGAGCA TTACATGTTC 
TTAAAGGGTC ATGAAACTTA CTCATATTAT GGGCCTCTGA ATTTACTTAC CTTCAATGTG 
GGTTATCATA ATGAACATCA TGATTTCCCC AACATTCCTG GAAAAAGTCT TCCACTGGTG 
AGGAAAATAG CAGCTGAATA CTATGACAAC CTCCCTCACT ACAATTCCTG GATAAAAGTA 
CTGTATGATT TTGTGATGGA TGATACAATA AGTCCCTACT CAAGAATGAA GAGGCACCAA 
AAAGGAGAGA TGGTGCTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308471.1
CDS	348..1211
Translation	MKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQKGEMVLE

Target ORF information:

RefSeq Version	NM_001321542.1
Organism	Homo sapiens(human)
Definition	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321542.1

ORF Insert Sequence:

ATGAAACCTG ATCCCAATTT GATATGGATT ATAATTATGA TGGTTCTCAC CCAGTTGGGT 
GCATTTTACA TAGTAAAAGA CTTGGACTGG AAATGGGTCA TATTTGGGGC CTATGCGTTT 
GGCAGTTGCA TTAACCACTC AATGACTCTG GCTATTCATG AGATTGCCCA CAATGCTGCC 
TTTGGCAACT GCAAAGCAAT GTGGAATCGC TGGTTTGGAA TGTTTGCTAA TCTTCCTATT 
GGGATTCCAT ATTCAATTTC CTTTAAGAGG TATCACATGG ATCATCATCG GTACCTTGGA 
GCTGATGGCG TCGATGTAGA TATTCCTACC GATTTTGAGG GCTGGTTCTT CTGTACCGCT 
TTCAGAAAGT TTATATGGGT TATTCTTCAG CCTCTCTTTT ATGCCTTTCG ACCTCTGTTC 
ATCAACCCCA AACCAATTAC GTATCTGGAA GTTATCAATA CCGTGGCACA GGTCACTTTT 
GACATTTTAA TTTATTACTT TTTGGGAATT AAATCCTTAG TCTACATGTT GGCAGCATCT 
TTACTTGGCC TGGGTTTGCA CCCAATTTCT GGACATTTTA TAGCTGAGCA TTACATGTTC 
TTAAAGGGTC ATGAAACTTA CTCATATTAT GGGCCTCTGA ATTTACTTAC CTTCAATGTG 
GGTTATCATA ATGAACATCA TGATTTCCCC AACATTCCTG GAAAAAGTCT TCCACTGGTG 
AGGAAAATAG CAGCTGAATA CTATGACAAC CTCCCTCACT ACAATTCCTG GATAAAAGTA 
CTGTATGATT TTGTGATGGA TGATACAATA AGTCCCTACT CAAGAATGAA GAGGCACCAA 
AAAGGAGAGA TGGTGCTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEGS1 ( NM_001321542.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308471.1 Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu61983
Clone ID Related Accession (Same CDS sequence)	XM_017002648.2 , NM_001321542.1 , XM_017002648.1 , NM_001321542.2
Accession Version	NM_001321542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	sphingolipid delta(4)-desaturase DES1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092809.2. On Jun 1, 2019 this sequence version replaced NM_001321542.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.189799.1, SRR6380199.74748.7 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAACCTG ATCCCAATTT GATATGGATT ATAATTATGA TGGTTCTCAC CCAGTTGGGT 
GCATTTTACA TAGTAAAAGA CTTGGACTGG AAATGGGTCA TATTTGGGGC CTATGCGTTT 
GGCAGTTGCA TTAACCACTC AATGACTCTG GCTATTCATG AGATTGCCCA CAATGCTGCC 
TTTGGCAACT GCAAAGCAAT GTGGAATCGC TGGTTTGGAA TGTTTGCTAA TCTTCCTATT 
GGGATTCCAT ATTCAATTTC CTTTAAGAGG TATCACATGG ATCATCATCG GTACCTTGGA 
GCTGATGGCG TCGATGTAGA TATTCCTACC GATTTTGAGG GCTGGTTCTT CTGTACCGCT 
TTCAGAAAGT TTATATGGGT TATTCTTCAG CCTCTCTTTT ATGCCTTTCG ACCTCTGTTC 
ATCAACCCCA AACCAATTAC GTATCTGGAA GTTATCAATA CCGTGGCACA GGTCACTTTT 
GACATTTTAA TTTATTACTT TTTGGGAATT AAATCCTTAG TCTACATGTT GGCAGCATCT 
TTACTTGGCC TGGGTTTGCA CCCAATTTCT GGACATTTTA TAGCTGAGCA TTACATGTTC 
TTAAAGGGTC ATGAAACTTA CTCATATTAT GGGCCTCTGA ATTTACTTAC CTTCAATGTG 
GGTTATCATA ATGAACATCA TGATTTCCCC AACATTCCTG GAAAAAGTCT TCCACTGGTG 
AGGAAAATAG CAGCTGAATA CTATGACAAC CTCCCTCACT ACAATTCCTG GATAAAAGTA 
CTGTATGATT TTGTGATGGA TGATACAATA AGTCCCTACT CAAGAATGAA GAGGCACCAA 
AAAGGAGAGA TGGTGCTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308471.1
CDS	179..1042
Translation	MKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQKGEMVLE

Target ORF information:

RefSeq Version	NM_001321542.2
Organism	Homo sapiens(human)
Definition	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321542.2

ORF Insert Sequence:

ATGAAACCTG ATCCCAATTT GATATGGATT ATAATTATGA TGGTTCTCAC CCAGTTGGGT 
GCATTTTACA TAGTAAAAGA CTTGGACTGG AAATGGGTCA TATTTGGGGC CTATGCGTTT 
GGCAGTTGCA TTAACCACTC AATGACTCTG GCTATTCATG AGATTGCCCA CAATGCTGCC 
TTTGGCAACT GCAAAGCAAT GTGGAATCGC TGGTTTGGAA TGTTTGCTAA TCTTCCTATT 
GGGATTCCAT ATTCAATTTC CTTTAAGAGG TATCACATGG ATCATCATCG GTACCTTGGA 
GCTGATGGCG TCGATGTAGA TATTCCTACC GATTTTGAGG GCTGGTTCTT CTGTACCGCT 
TTCAGAAAGT TTATATGGGT TATTCTTCAG CCTCTCTTTT ATGCCTTTCG ACCTCTGTTC 
ATCAACCCCA AACCAATTAC GTATCTGGAA GTTATCAATA CCGTGGCACA GGTCACTTTT 
GACATTTTAA TTTATTACTT TTTGGGAATT AAATCCTTAG TCTACATGTT GGCAGCATCT 
TTACTTGGCC TGGGTTTGCA CCCAATTTCT GGACATTTTA TAGCTGAGCA TTACATGTTC 
TTAAAGGGTC ATGAAACTTA CTCATATTAT GGGCCTCTGA ATTTACTTAC CTTCAATGTG 
GGTTATCATA ATGAACATCA TGATTTCCCC AACATTCCTG GAAAAAGTCT TCCACTGGTG 
AGGAAAATAG CAGCTGAATA CTATGACAAC CTCCCTCACT ACAATTCCTG GATAAAAGTA 
CTGTATGATT TTGTGATGGA TGATACAATA AGTCCCTACT CAAGAATGAA GAGGCACCAA 
AAAGGAGAGA TGGTGCTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEGS1 ( XM_017002648.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858137.1 Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61983
Clone ID Related Accession (Same CDS sequence)	XM_017002648.2 , NM_001321542.1 , XM_017002648.1 , NM_001321542.2
Accession Version	XM_017002648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	sphingolipid delta(4)-desaturase DES1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTG ATCCCAATTT GATATGGATT ATAATTATGA TGGTTCTCAC CCAGTTGGGT 
GCATTTTACA TAGTAAAAGA CTTGGACTGG AAATGGGTCA TATTTGGGGC CTATGCGTTT 
GGCAGTTGCA TTAACCACTC AATGACTCTG GCTATTCATG AGATTGCCCA CAATGCTGCC 
TTTGGCAACT GCAAAGCAAT GTGGAATCGC TGGTTTGGAA TGTTTGCTAA TCTTCCTATT 
GGGATTCCAT ATTCAATTTC CTTTAAGAGG TATCACATGG ATCATCATCG GTACCTTGGA 
GCTGATGGCG TCGATGTAGA TATTCCTACC GATTTTGAGG GCTGGTTCTT CTGTACCGCT 
TTCAGAAAGT TTATATGGGT TATTCTTCAG CCTCTCTTTT ATGCCTTTCG ACCTCTGTTC 
ATCAACCCCA AACCAATTAC GTATCTGGAA GTTATCAATA CCGTGGCACA GGTCACTTTT 
GACATTTTAA TTTATTACTT TTTGGGAATT AAATCCTTAG TCTACATGTT GGCAGCATCT 
TTACTTGGCC TGGGTTTGCA CCCAATTTCT GGACATTTTA TAGCTGAGCA TTACATGTTC 
TTAAAGGGTC ATGAAACTTA CTCATATTAT GGGCCTCTGA ATTTACTTAC CTTCAATGTG 
GGTTATCATA ATGAACATCA TGATTTCCCC AACATTCCTG GAAAAAGTCT TCCACTGGTG 
AGGAAAATAG CAGCTGAATA CTATGACAAC CTCCCTCACT ACAATTCCTG GATAAAAGTA 
CTGTATGATT TTGTGATGGA TGATACAATA AGTCCCTACT CAAGAATGAA GAGGCACCAA 
AAAGGAGAGA TGGTGCTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858137.1
CDS	278..1141
Translation	MKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQKGEMVLE

Target ORF information:

RefSeq Version	XM_017002648.2
Organism	Homo sapiens(human)
Definition	Homo sapiens delta 4-desaturase, sphingolipid 1 (DEGS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002648.2

ORF Insert Sequence:

ATGAAACCTG ATCCCAATTT GATATGGATT ATAATTATGA TGGTTCTCAC CCAGTTGGGT 
GCATTTTACA TAGTAAAAGA CTTGGACTGG AAATGGGTCA TATTTGGGGC CTATGCGTTT 
GGCAGTTGCA TTAACCACTC AATGACTCTG GCTATTCATG AGATTGCCCA CAATGCTGCC 
TTTGGCAACT GCAAAGCAAT GTGGAATCGC TGGTTTGGAA TGTTTGCTAA TCTTCCTATT 
GGGATTCCAT ATTCAATTTC CTTTAAGAGG TATCACATGG ATCATCATCG GTACCTTGGA 
GCTGATGGCG TCGATGTAGA TATTCCTACC GATTTTGAGG GCTGGTTCTT CTGTACCGCT 
TTCAGAAAGT TTATATGGGT TATTCTTCAG CCTCTCTTTT ATGCCTTTCG ACCTCTGTTC 
ATCAACCCCA AACCAATTAC GTATCTGGAA GTTATCAATA CCGTGGCACA GGTCACTTTT 
GACATTTTAA TTTATTACTT TTTGGGAATT AAATCCTTAG TCTACATGTT GGCAGCATCT 
TTACTTGGCC TGGGTTTGCA CCCAATTTCT GGACATTTTA TAGCTGAGCA TTACATGTTC 
TTAAAGGGTC ATGAAACTTA CTCATATTAT GGGCCTCTGA ATTTACTTAC CTTCAATGTG 
GGTTATCATA ATGAACATCA TGATTTCCCC AACATTCCTG GAAAAAGTCT TCCACTGGTG 
AGGAAAATAG CAGCTGAATA CTATGACAAC CTCCCTCACT ACAATTCCTG GATAAAAGTA 
CTGTATGATT TTGTGATGGA TGATACAATA AGTCCCTACT CAAGAATGAA GAGGCACCAA 
AAAGGAGAGA TGGTGCTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DEGS1 ( XM_017002648.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858137.1 Homo sapiens delta(4)-desaturase, sphingolipid 1 (DEGS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu61983
Clone ID Related Accession (Same CDS sequence)	XM_017002648.2 , NM_001321542.1 , XM_017002648.1 , NM_001321542.2
Accession Version	XM_017002648.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sphingolipid delta(4)-desaturase DES1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACCTG ATCCCAATTT GATATGGATT ATAATTATGA TGGTTCTCAC CCAGTTGGGT 
GCATTTTACA TAGTAAAAGA CTTGGACTGG AAATGGGTCA TATTTGGGGC CTATGCGTTT 
GGCAGTTGCA TTAACCACTC AATGACTCTG GCTATTCATG AGATTGCCCA CAATGCTGCC 
TTTGGCAACT GCAAAGCAAT GTGGAATCGC TGGTTTGGAA TGTTTGCTAA TCTTCCTATT 
GGGATTCCAT ATTCAATTTC CTTTAAGAGG TATCACATGG ATCATCATCG GTACCTTGGA 
GCTGATGGCG TCGATGTAGA TATTCCTACC GATTTTGAGG GCTGGTTCTT CTGTACCGCT 
TTCAGAAAGT TTATATGGGT TATTCTTCAG CCTCTCTTTT ATGCCTTTCG ACCTCTGTTC 
ATCAACCCCA AACCAATTAC GTATCTGGAA GTTATCAATA CCGTGGCACA GGTCACTTTT 
GACATTTTAA TTTATTACTT TTTGGGAATT AAATCCTTAG TCTACATGTT GGCAGCATCT 
TTACTTGGCC TGGGTTTGCA CCCAATTTCT GGACATTTTA TAGCTGAGCA TTACATGTTC 
TTAAAGGGTC ATGAAACTTA CTCATATTAT GGGCCTCTGA ATTTACTTAC CTTCAATGTG 
GGTTATCATA ATGAACATCA TGATTTCCCC AACATTCCTG GAAAAAGTCT TCCACTGGTG 
AGGAAAATAG CAGCTGAATA CTATGACAAC CTCCCTCACT ACAATTCCTG GATAAAAGTA 
CTGTATGATT TTGTGATGGA TGATACAATA AGTCCCTACT CAAGAATGAA GAGGCACCAA 
AAAGGAGAGA TGGTGCTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858137.1
CDS	278..1141
Translation	MKPDPNLIWIIIMMVLTQLGAFYIVKDLDWKWVIFGAYAFGSCINHSMTLAIHEIAHNAAFGNCKAMWNRWFGMFANLPIGIPYSISFKRYHMDHHRYLGADGVDVDIPTDFEGWFFCTAFRKFIWVILQPLFYAFRPLFINPKPITYLEVINTVAQVTFDILIYYFLGIKSLVYMLAASLLGLGLHPISGHFIAEHYMFLKGHETYSYYGPLNLLTFNVGYHNEHHDFPNIPGKSLPLVRKIAAEYYDNLPHYNSWIKVLYDFVMDDTISPYSRMKRHQKGEMVLE

Target ORF information:

RefSeq Version	XM_017002648.1
Organism	Homo sapiens(human)
Definition	Homo sapiens delta(4)-desaturase, sphingolipid 1 (DEGS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002648.1

ORF Insert Sequence:

ATGAAACCTG ATCCCAATTT GATATGGATT ATAATTATGA TGGTTCTCAC CCAGTTGGGT 
GCATTTTACA TAGTAAAAGA CTTGGACTGG AAATGGGTCA TATTTGGGGC CTATGCGTTT 
GGCAGTTGCA TTAACCACTC AATGACTCTG GCTATTCATG AGATTGCCCA CAATGCTGCC 
TTTGGCAACT GCAAAGCAAT GTGGAATCGC TGGTTTGGAA TGTTTGCTAA TCTTCCTATT 
GGGATTCCAT ATTCAATTTC CTTTAAGAGG TATCACATGG ATCATCATCG GTACCTTGGA 
GCTGATGGCG TCGATGTAGA TATTCCTACC GATTTTGAGG GCTGGTTCTT CTGTACCGCT 
TTCAGAAAGT TTATATGGGT TATTCTTCAG CCTCTCTTTT ATGCCTTTCG ACCTCTGTTC 
ATCAACCCCA AACCAATTAC GTATCTGGAA GTTATCAATA CCGTGGCACA GGTCACTTTT 
GACATTTTAA TTTATTACTT TTTGGGAATT AAATCCTTAG TCTACATGTT GGCAGCATCT 
TTACTTGGCC TGGGTTTGCA CCCAATTTCT GGACATTTTA TAGCTGAGCA TTACATGTTC 
TTAAAGGGTC ATGAAACTTA CTCATATTAT GGGCCTCTGA ATTTACTTAC CTTCAATGTG 
GGTTATCATA ATGAACATCA TGATTTCCCC AACATTCCTG GAAAAAGTCT TCCACTGGTG 
AGGAAAATAG CAGCTGAATA CTATGACAAC CTCCCTCACT ACAATTCCTG GATAAAAGTA 
CTGTATGATT TTGTGATGGA TGATACAATA AGTCCCTACT CAAGAATGAA GAGGCACCAA 
AAAGGAGAGA TGGTGCTGGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dihydroceramide-desaturase-1-mediated caspase 9 activation through ceramide plays a pivotal role in palmitic acid-induced HepG2 cell apoptosis.
Apoptosis : an international journal on programmed cell death21(9)1033-44(2016 Sep)
Zhu Q,Yang J,Zhu R,Jiang X,Li W,He S,Jin J

The Sphingosine Kinase 2 Inhibitor ABC294640 Reduces the Growth of Prostate Cancer Cells and Results in Accumulation of Dihydroceramides In Vitro and In Vivo.
Molecular cancer therapeutics14(12)2744-52(2015 Dec)
Venant H,Rahmaniyan M,Jones EE,Lu P,Lilly MB,Garrett-Mayer E,Drake RR,Kraveka JM,Smith CD,Voelkel-Johnson C

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DEGS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

オンラインフォーム