SNRPA1 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SNRPA1
Entrez Gene ID	6627
Full Name	small nuclear ribonucleoprotein polypeptide A'
Synonyms	Lea1
General protein information	Preferred Names small nuclear ribonucleoprotein polypeptide A' Names U2 small nuclear ribonucleoprotein A' U2 small nuclear ribonucleoprotein polypeptide A' U2 snRNP A'
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 15 Map Location: 15q26.3
Disorder MIM:	603521

mRNA and Protein(s)

mRNA	Protein	Name
NM_003090.4	NP_003081.2	U2 small nuclear ribonucleoprotein A'
NM_003090.3	NP_003081.2	U2 small nuclear ribonucleoprotein A'
NM_003090.2	NP_003081.2	U2 small nuclear ribonucleoprotein A'

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SNRPA1	XP_510625.2
Mus musculus (house mouse)	Snrpa1	NP_067311.4
Drosophila melanogaster (fruit fly)	U2A	NP_610315.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007670	XP_001687866.1
Neurospora crassa	NCU06622	XP_962364.1
Arabidopsis thaliana (thale cress)	U2A'	NP_172447.1
Canis lupus familiaris (dog)	SNRPA1	XP_848923.1
Gallus gallus (chicken)	SNRPA1	NP_001005823.1
Magnaporthe oryzae (rice blast fungus)	MGG_13209	XP_003711971.1
	SNRPA1	XP_001085262.1
Bos taurus (cattle)	SNRPA1	NP_001092418.1
Caenorhabditis elegans (roundworm)	mog-2	NP_494763.1
Schizosaccharomyces pombe (fission yeast)	lea1	NP_596725.1
Xenopus tropicalis (tropical clawed frog)	snrpa1	XP_002939312.2
Homo sapiens (human)	SNRPA1	NP_003081.2
Rattus norvegicus (Norway rat)	Snrpa1	NP_001258252.1
Danio rerio (zebrafish)	snrpa1	NP_001007384.2
Oryza sativa (rice)	Os02g0234800	NP_001046383.1

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

The unique spliceosome signature of human pluripotent stem cells is mediated by SNRPA1, SNRPD1, and PNN.
Kim YD, Lee J, Kim HS, Lee MO, Son MY, Yoo CH, Choi JK, Lee SC, Cho YS
Stem cell research2243-53(2017 Jul)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

Major spliceosome defects cause male infertility and are associated with nonobstructive azoospermia in humans.
Wu H, Sun L, Wen Y, Liu Y, Yu J, Mao F, Wang Y, Tong C, Guo X, Hu Z, Sha J, Liu M, Xia L
Proceedings of the National Academy of Sciences of the United States of America113(15)4134-9(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that protein-protein interactions localize U2A' (SNRPA1) to the U2 snRNP (U2 small nuclear ribonucleoprotein) and exclude it from the U1 snRNP, rather than to enhance U2 snRNA (U2 small nuclear RNA; RNU2-1) binding of U2B'' (SNRPB2).
Title: Binding affinity and cooperativity control U2B?/snRNA/U2A' RNP formation.

PMID:10811961 pertains mostly to salmon, but has some reference to human.
Title: Gene Structure of the U2 snRNP-Specific A' Protein Gene from Salmo salar: Alternative Transcripts Observed.

The following SNRPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SNRPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14681	NM_003090.4 Latest version!	Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu14681	NM_003090.3	Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14681	NM_003090.2	Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SNRPA1 ( NM_003090.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003081.2 Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14681
Clone ID Related Accession (Same CDS sequence)	NM_003090.4 , NM_003090.3 , NM_003090.2
Accession Version	NM_003090.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	U2 small nuclear ribonucleoprotein A'
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC023024.6, AK312200.1 and BC022816.1. On Nov 23, 2018 this sequence version replaced NM_003090.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.21692.1, SRR1163658.513763.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000254193.11/ ENSP00000254193.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTCAAGC TGACGGCGGA GCTGATCGAG CAGGCGGCGC AGTACACCAA CGCGGTGCGC 
GACCGGGAGC TGGACCTCCG GGGGTATAAA ATTCCCGTCA TTGAAAATCT AGGTGCTACG 
TTAGACCAGT TTGATGCTAT TGATTTTTCT GACAATGAGA TCAGGAAACT GGATGGTTTT 
CCTTTGTTGA GAAGACTGAA AACATTGTTA GTGAACAACA ACAGAATATG CCGTATAGGT 
GAGGGACTTG ATCAGGCTCT GCCCTGTCTG ACAGAACTCA TTCTCACCAA TAATAGTCTC 
GTGGAACTGG GTGATCTGGA CCCTCTGGCA TCTCTCAAAT CGCTGACTTA CCTAAGTATC 
CTAAGAAATC CGGTAACCAA TAAGAAGCAT TACAGATTGT ATGTGATTTA TAAAGTTCCG 
CAAGTCAGAG TACTGGATTT CCAGAAAGTG AAACTAAAAG AGCGTCAGGA AGCAGAGAAA 
ATGTTCAAGG GCAAACGGGG TGCACAGCTT GCAAAGGATA TTGCCAGGAG AAGCAAAACT 
TTTAATCCAG GTGCTGGTTT GCCAACTGAC AAAAAGAAAG GTGGGCCATC TCCAGGGGAT 
GTAGAAGCAA TCAAGAATGC CATAGCAAAT GCTTCAACTC TGGCTGAAGT GGAGAGGCTG 
AAGGGGTTGC TGCAGTCTGG TCAGATCCCT GGCAGAGAAC GCAGATCAGG GCCCACTGAT 
GATGGTGAAG AAGAGATGGA AGAAGACACA GTCACAAACG GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003081.2
CDS	71..838
Translation	MVKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIGEGLDQALPCLTELILTNNSLVELGDLDPLASLKSLTYLSILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKTFNPGAGLPTDKKKGGPSPGDVEAIKNAIANASTLAEVERLKGLLQSGQIPGRERRSGPTDDGEEEMEEDTVTNGS

Target ORF information:

RefSeq Version	NM_003090.4
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003090.4

ORF Insert Sequence:

ATGGTCAAGC TGACGGCGGA GCTGATCGAG CAGGCGGCGC AGTACACCAA CGCGGTGCGC 
GACCGGGAGC TGGACCTCCG GGGGTATAAA ATTCCCGTCA TTGAAAATCT AGGTGCTACG 
TTAGACCAGT TTGATGCTAT TGATTTTTCT GACAATGAGA TCAGGAAACT GGATGGTTTT 
CCTTTGTTGA GAAGACTGAA AACATTGTTA GTGAACAACA ACAGAATATG CCGTATAGGT 
GAGGGACTTG ATCAGGCTCT GCCCTGTCTG ACAGAACTCA TTCTCACCAA TAATAGTCTC 
GTGGAACTGG GTGATCTGGA CCCTCTGGCA TCTCTCAAAT CGCTGACTTA CCTAAGTATC 
CTAAGAAATC CGGTAACCAA TAAGAAGCAT TACAGATTGT ATGTGATTTA TAAAGTTCCG 
CAAGTCAGAG TACTGGATTT CCAGAAAGTG AAACTAAAAG AGCGTCAGGA AGCAGAGAAA 
ATGTTCAAGG GCAAACGGGG TGCACAGCTT GCAAAGGATA TTGCCAGGAG AAGCAAAACT 
TTTAATCCAG GTGCTGGTTT GCCAACTGAC AAAAAGAAAG GTGGGCCATC TCCAGGGGAT 
GTAGAAGCAA TCAAGAATGC CATAGCAAAT GCTTCAACTC TGGCTGAAGT GGAGAGGCTG 
AAGGGGTTGC TGCAGTCTGG TCAGATCCCT GGCAGAGAAC GCAGATCAGG GCCCACTGAT 
GATGGTGAAG AAGAGATGGA AGAAGACACA GTCACAAACG GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPA1 ( NM_003090.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003081.2 Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14681
Clone ID Related Accession (Same CDS sequence)	NM_003090.4 , NM_003090.3 , NM_003090.2
Accession Version	NM_003090.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	U2 small nuclear ribonucleoprotein A'
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC023024.6, AK312200.1 and BC022816.1. On Mar 12, 2016 this sequence version replaced NM_003090.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.143899.1, SRR1163658.513763.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGTCAAGC TGACGGCGGA GCTGATCGAG CAGGCGGCGC AGTACACCAA CGCGGTGCGC 
GACCGGGAGC TGGACCTCCG GGGGTATAAA ATTCCCGTCA TTGAAAATCT AGGTGCTACG 
TTAGACCAGT TTGATGCTAT TGATTTTTCT GACAATGAGA TCAGGAAACT GGATGGTTTT 
CCTTTGTTGA GAAGACTGAA AACATTGTTA GTGAACAACA ACAGAATATG CCGTATAGGT 
GAGGGACTTG ATCAGGCTCT GCCCTGTCTG ACAGAACTCA TTCTCACCAA TAATAGTCTC 
GTGGAACTGG GTGATCTGGA CCCTCTGGCA TCTCTCAAAT CGCTGACTTA CCTAAGTATC 
CTAAGAAATC CGGTAACCAA TAAGAAGCAT TACAGATTGT ATGTGATTTA TAAAGTTCCG 
CAAGTCAGAG TACTGGATTT CCAGAAAGTG AAACTAAAAG AGCGTCAGGA AGCAGAGAAA 
ATGTTCAAGG GCAAACGGGG TGCACAGCTT GCAAAGGATA TTGCCAGGAG AAGCAAAACT 
TTTAATCCAG GTGCTGGTTT GCCAACTGAC AAAAAGAAAG GTGGGCCATC TCCAGGGGAT 
GTAGAAGCAA TCAAGAATGC CATAGCAAAT GCTTCAACTC TGGCTGAAGT GGAGAGGCTG 
AAGGGGTTGC TGCAGTCTGG TCAGATCCCT GGCAGAGAAC GCAGATCAGG GCCCACTGAT 
GATGGTGAAG AAGAGATGGA AGAAGACACA GTCACAAACG GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003081.2
CDS	105..872
Translation	MVKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIGEGLDQALPCLTELILTNNSLVELGDLDPLASLKSLTYLSILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKTFNPGAGLPTDKKKGGPSPGDVEAIKNAIANASTLAEVERLKGLLQSGQIPGRERRSGPTDDGEEEMEEDTVTNGS

Target ORF information:

RefSeq Version	NM_003090.3
Organism	Homo sapiens(human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003090.3

ORF Insert Sequence:

ATGGTCAAGC TGACGGCGGA GCTGATCGAG CAGGCGGCGC AGTACACCAA CGCGGTGCGC 
GACCGGGAGC TGGACCTCCG GGGGTATAAA ATTCCCGTCA TTGAAAATCT AGGTGCTACG 
TTAGACCAGT TTGATGCTAT TGATTTTTCT GACAATGAGA TCAGGAAACT GGATGGTTTT 
CCTTTGTTGA GAAGACTGAA AACATTGTTA GTGAACAACA ACAGAATATG CCGTATAGGT 
GAGGGACTTG ATCAGGCTCT GCCCTGTCTG ACAGAACTCA TTCTCACCAA TAATAGTCTC 
GTGGAACTGG GTGATCTGGA CCCTCTGGCA TCTCTCAAAT CGCTGACTTA CCTAAGTATC 
CTAAGAAATC CGGTAACCAA TAAGAAGCAT TACAGATTGT ATGTGATTTA TAAAGTTCCG 
CAAGTCAGAG TACTGGATTT CCAGAAAGTG AAACTAAAAG AGCGTCAGGA AGCAGAGAAA 
ATGTTCAAGG GCAAACGGGG TGCACAGCTT GCAAAGGATA TTGCCAGGAG AAGCAAAACT 
TTTAATCCAG GTGCTGGTTT GCCAACTGAC AAAAAGAAAG GTGGGCCATC TCCAGGGGAT 
GTAGAAGCAA TCAAGAATGC CATAGCAAAT GCTTCAACTC TGGCTGAAGT GGAGAGGCTG 
AAGGGGTTGC TGCAGTCTGG TCAGATCCCT GGCAGAGAAC GCAGATCAGG GCCCACTGAT 
GATGGTGAAG AAGAGATGGA AGAAGACACA GTCACAAACG GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SNRPA1 ( NM_003090.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_003081.2 Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), mRNA.

Price & Availability↑

CloneID	OHu14681
Clone ID Related Accession (Same CDS sequence)	NM_003090.4 , NM_003090.3 , NM_003090.2
Accession Version	NM_003090.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 768bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	U2 small nuclear ribonucleoprotein A'
Comment

ATGGTCAAGC TGACGGCGGA GCTGATCGAG CAGGCGGCGC AGTACACCAA CGCGGTGCGC 
GACCGGGAGC TGGACCTCCG GGGGTATAAA ATTCCCGTCA TTGAAAATCT AGGTGCTACG 
TTAGACCAGT TTGATGCTAT TGATTTTTCT GACAATGAGA TCAGGAAACT GGATGGTTTT 
CCTTTGTTGA GAAGACTGAA AACATTGTTA GTGAACAACA ACAGAATATG CCGTATAGGT 
GAGGGACTTG ATCAGGCTCT GCCCTGTCTG ACAGAACTCA TTCTCACCAA TAATAGTCTC 
GTGGAACTGG GTGATCTGGA CCCTCTGGCA TCTCTCAAAT CGCTGACTTA CCTAAGTATC 
CTAAGAAATC CGGTAACCAA TAAGAAGCAT TACAGATTGT ATGTGATTTA TAAAGTTCCG 
CAAGTCAGAG TACTGGATTT CCAGAAAGTG AAACTAAAAG AGCGTCAGGA AGCAGAGAAA 
ATGTTCAAGG GCAAACGGGG TGCACAGCTT GCAAAGGATA TTGCCAGGAG AAGCAAAACT 
TTTAATCCAG GTGCTGGTTT GCCAACTGAC AAAAAGAAAG GTGGGCCATC TCCAGGGGAT 
GTAGAAGCAA TCAAGAATGC CATAGCAAAT GCTTCAACTC TGGCTGAAGT GGAGAGGCTG 
AAGGGGTTGC TGCAGTCTGG TCAGATCCCT GGCAGAGAAC GCAGATCAGG GCCCACTGAT 
GATGGTGAAG AAGAGATGGA AGAAGACACA GTCACAAACG GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003081.2
Translation	MVKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIGEGLDQALPCLTELILTNNSLVELGDLDPLASLKSLTYLSILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKTFNPGAGLPTDKKKGGPSPGDVEAIKNAIANASTLAEVERLKGLLQSGQIPGRERRSGPTDDGEEEMEEDTVTNGS

Target ORF information:

RefSeq Version	NM_003090.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens small nuclear ribonucleoprotein polypeptide A' (SNRPA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003090.2

ORF Insert Sequence:

ATGGTCAAGC TGACGGCGGA GCTGATCGAG CAGGCGGCGC AGTACACCAA CGCGGTGCGC 
GACCGGGAGC TGGACCTCCG GGGGTATAAA ATTCCCGTCA TTGAAAATCT AGGTGCTACG 
TTAGACCAGT TTGATGCTAT TGATTTTTCT GACAATGAGA TCAGGAAACT GGATGGTTTT 
CCTTTGTTGA GAAGACTGAA AACATTGTTA GTGAACAACA ACAGAATATG CCGTATAGGT 
GAGGGACTTG ATCAGGCTCT GCCCTGTCTG ACAGAACTCA TTCTCACCAA TAATAGTCTC 
GTGGAACTGG GTGATCTGGA CCCTCTGGCA TCTCTCAAAT CGCTGACTTA CCTAAGTATC 
CTAAGAAATC CGGTAACCAA TAAGAAGCAT TACAGATTGT ATGTGATTTA TAAAGTTCCG 
CAAGTCAGAG TACTGGATTT CCAGAAAGTG AAACTAAAAG AGCGTCAGGA AGCAGAGAAA 
ATGTTCAAGG GCAAACGGGG TGCACAGCTT GCAAAGGATA TTGCCAGGAG AAGCAAAACT 
TTTAATCCAG GTGCTGGTTT GCCAACTGAC AAAAAGAAAG GTGGGCCATC TCCAGGGGAT 
GTAGAAGCAA TCAAGAATGC CATAGCAAAT GCTTCAACTC TGGCTGAAGT GGAGAGGCTG 
AAGGGGTTGC TGCAGTCTGG TCAGATCCCT GGCAGAGAAC GCAGATCAGG GCCCACTGAT 
GATGGTGAAG AAGAGATGGA AGAAGACACA GTCACAAACG GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H

The unique spliceosome signature of human pluripotent stem cells is mediated by SNRPA1, SNRPD1, and PNN.
Stem cell research2243-53(2017 Jul)
Kim YD,Lee J,Kim HS,Lee MO,Son MY,Yoo CH,Choi JK,Lee SC,Cho YS

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R

Major spliceosome defects cause male infertility and are associated with nonobstructive azoospermia in humans.
Proceedings of the National Academy of Sciences of the United States of America113(15)4134-9(2016 Apr)
Wu H,Sun L,Wen Y,Liu Y,Yu J,Mao F,Wang Y,Tong C,Guo X,Hu Z,Sha J,Liu M,Xia L

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SNRPA1 cDNA ORF clone, Homo sapiens(Human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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