ST8SIA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ST8SIA1
Entrez Gene ID	6489
Full Name	ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
Synonyms	GD3S,SIAT8,SIAT8-A,SIAT8A,ST8SiaI
General protein information	Preferred Names ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 Names alpha-N-acetylneuraminide alpha-2,8-sialyltransferase ST8 alpha-N-acetylneuraminate alpha-2,8-sialyltransferase 1 alpha-2,8-sialyltransferase 8A disialoganglioside (GD3) synthase ganglioside GD3 synthase ganglioside GT3 synthase ganglioside-specific alpha-2,8-polysialyltransferase sialyltransferase 8 (alpha-N-acetylneuraminate: alpha-2,8-sialytransferase, GD3 synthase) A sialyltransferase 8A (alpha-N-acetylneuraminate: alpha-2,8-sialyltransferase, GD3 synthase) sialyltransferase St8Sia I sialytransferase St8Sia I
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p12.1
Summary	Gangliosides are membrane-bound glycosphingolipids containing sialic acid. Ganglioside GD3 is known to be important for cell adhesion and growth of cultured malignant cells. The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce gangliosides GD3 and GT3. The encoded protein may be found in the Golgi apparatus and is a member of glycosyltransferase family 29. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2015].
Disorder MIM:	601123

mRNA and Protein(s)

mRNA	Protein	Name
NM_003034.3	NP_003025.1	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase isoform 1
NM_001304450.1	NP_001291379.1	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ST8SIA1	NP_003025.1
	ST8SIA1	XP_001099511.1
Bos taurus (cattle)	ST8SIA1	NP_001027470.1
Gallus gallus (chicken)	ST8SIA1	NP_990339.1
Canis lupus familiaris (dog)	ST8SIA1	XP_534865.2
Pan troglodytes (chimpanzee)	ST8SIA1	NP_001032380.1
Mus musculus (house mouse)	St8sia1	NP_035504.2
Rattus norvegicus (Norway rat)	St8sia1	NP_036945.2
Danio rerio (zebrafish)	LOC100333872	XP_002661434.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification and Functional Characterization of ST3GAL5 and ST8SIA1 Variants in Patients with Thyroid-Associated Ophthalmopathy.
Park HJ, Kim JH, Yoon JS, Choi YJ, Choi YH, Kook KH, Choi JH
Yonsei medical journal58(6)1160-1169(2017 Nov)

MicroRNA-33a and let-7e inhibit human colorectal cancer progression by targeting ST8SIA1.
Shan Y, Liu Y, Zhao L, Liu B, Li Y, Jia L
The international journal of biochemistry & cell biology9048-58(2017 Sep)

Ganglioside glycosyltransferases are S-acylated at conserved cysteine residues involved in homodimerisation.
Chumpen Ramirez S, Ruggiero FM, Daniotti JL, Valdez Taubas J
The Biochemical journal474(16)2803-2816(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results from genotype-phenotype analysis might suggest a possible link between the ST8SIA1 functional promoter haplotype and the clinical severity of TAO [thyroid-associated ophthalmopathy].
Title: Identification and Functional Characterization of ST3GAL5 and ST8SIA1 Variants in Patients with Thyroid-Associated Ophthalmopathy.

altered expression of miR-33a/let-7e was correlated with ST8SIA1 level, which might contribute to CRC progression
Title: MicroRNA-33a and let-7e inhibit human colorectal cancer progression by targeting ST8SIA1.

Data suggest that ganglioside glycosyltransferases ST3GAL5, ST8SIA1, and B4GALNT1 are S-acylated at conserved cysteine residues located close to cytoplasmic border of their transmembrane domains; ST3Gal-II is acylated at conserved cysteine residue in N-terminal cytoplasmic tail; for B4GALNT1 and ST3Gal-II, dimer formation controls their S-acylation status.
Title: Ganglioside glycosyltransferases are S-acylated at conserved cysteine residues involved in homodimerisation.

GD3 and GD3S are highly expressed in glioblastoma multiforme (GBM) stem cell, play a key role in glioblastoma tumorigenicity, and are potential therapeutic targets against GBM.
Title: Glycolipid GD3 and GD3 synthase are key drivers for glioblastoma stem cells and tumorigenicity.

Though results are limited by the small sample size, variants in BDNF and ST8SIA may slow down the early response to antidepressants in subjects non-exposed to stressors at the illness onset, with a remarkable gene-environment interaction
Title: Genes involved in neuroplasticity and stressful life events act on the short-term response to antidepressant treatment: a complex interplay between genetics and environment.

The following ST8SIA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ST8SIA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu04050	NM_003034.3 Latest version!	Homo sapiens ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (ST8SIA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu46170	NM_001304450.1 Latest version!	Homo sapiens ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (ST8SIA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ST8SIA1 ( NM_003034.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003025.1 Homo sapiens ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (ST8SIA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04050
Clone ID Related Accession (Same CDS sequence)	NM_003034.3
Accession Version	NM_003034.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X77922.1, BC117285.1, AC007671.7 and BM929838.1. On Dec 20, 2007 this sequence version replaced NM_003034.2. Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X77922.1, SRR1660809.3107.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCCCCT GCGGGCGGGC CCGGCGACAA ACGTCCAGAG GGGCCATGGC TGTACTGGCG 
TGGAAGTTCC CGCGGACCCG GCTGCCCATG GGAGCCAGTG CCCTCTGTGT CGTGGTCCTC 
TGTTGGCTCT ACATCTTCCC CGTCTACCGG CTGCCCAACG AGAAAGAGAT CGTGCAGGGG 
GTGCTGCAAC AGGGCACGGC GTGGAGGAGG AACCAGACCG CGGCCAGAGC GTTCAGGAAA 
CAAATGGAAG ACTGCTGCGA CCCTGCCCAT CTCTTTGCTA TGACTAAAAT GAATTCCCCT 
ATGGGGAAGA GCATGTGGTA TGACGGGGAG TTTTTATACT CATTCACCAT TGACAATTCA 
ACTTACTCTC TCTTCCCACA GGCAACCCCA TTCCAGCTGC CATTGAAGAA ATGCGCGGTG 
GTGGGAAATG GTGGGATTCT GAAGAAGAGT GGCTGTGGCC GTCAAATAGA TGAAGCAAAT 
TTTGTCATGC GATGCAATCT CCCTCCTTTG TCAAGTGAAT ACACTAAGGA TGTTGGATCC 
AAAAGTCAGT TAGTGACAGC TAATCCCAGC ATAATTCGGC AAAGGTTTCA GAACCTTCTG 
TGGTCCAGAA AGACATTTGT GGACAACATG AAAATTTATA ACCACAGTTA CATCTACATG 
CCTGCCTTTT CTATGAAGAC AGGAACAGAG CCATCTTTGA GGGTTTATTA TACACTGTCA 
GATGTTGGTG CCAATCAAAC AGTGCTGTTT GCCAACCCCA ACTTTCTGCG TAGCATTGGA 
AAGTTCTGGA AAAGTAGAGG AATCCATGCC AAGCGCCTGT CCACAGGACT TTTTCTGGTG 
AGCGCAGCTC TGGGTCTCTG TGAAGAGGTG GCCATCTATG GCTTCTGGCC CTTCTCTGTG 
AATATGCATG AGCAGCCCAT CAGCCACCAC TACTATGACA ACGTCTTACC CTTTTCTGGC 
TTCCATGCCA TGCCCGAGGA ATTTCTCCAA CTCTGGTATC TTCATAAAAT CGGTGCACTG 
AGAATGCAGC TGGACCCATG TGAAGATACC TCACTCCAGC CCACTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003025.1
CDS	483..1553
Translation	MSPCGRARRQTSRGAMAVLAWKFPRTRLPMGASALCVVVLCWLYIFPVYRLPNEKEIVQGVLQQGTAWRRNQTAARAFRKQMEDCCDPAHLFAMTKMNSPMGKSMWYDGEFLYSFTIDNSTYSLFPQATPFQLPLKKCAVVGNGGILKKSGCGRQIDEANFVMRCNLPPLSSEYTKDVGSKSQLVTANPSIIRQRFQNLLWSRKTFVDNMKIYNHSYIYMPAFSMKTGTEPSLRVYYTLSDVGANQTVLFANPNFLRSIGKFWKSRGIHAKRLSTGLFLVSAALGLCEEVAIYGFWPFSVNMHEQPISHHYYDNVLPFSGFHAMPEEFLQLWYLHKIGALRMQLDPCEDTSLQPTS

Target ORF information:

RefSeq Version	NM_003034.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (ST8SIA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003034.3

ORF Insert Sequence:

ATGAGCCCCT GCGGGCGGGC CCGGCGACAA ACGTCCAGAG GGGCCATGGC TGTACTGGCG 
TGGAAGTTCC CGCGGACCCG GCTGCCCATG GGAGCCAGTG CCCTCTGTGT CGTGGTCCTC 
TGTTGGCTCT ACATCTTCCC CGTCTACCGG CTGCCCAACG AGAAAGAGAT CGTGCAGGGG 
GTGCTGCAAC AGGGCACGGC GTGGAGGAGG AACCAGACCG CGGCCAGAGC GTTCAGGAAA 
CAAATGGAAG ACTGCTGCGA CCCTGCCCAT CTCTTTGCTA TGACTAAAAT GAATTCCCCT 
ATGGGGAAGA GCATGTGGTA TGACGGGGAG TTTTTATACT CATTCACCAT TGACAATTCA 
ACTTACTCTC TCTTCCCACA GGCAACCCCA TTCCAGCTGC CATTGAAGAA ATGCGCGGTG 
GTGGGAAATG GTGGGATTCT GAAGAAGAGT GGCTGTGGCC GTCAAATAGA TGAAGCAAAT 
TTTGTCATGC GATGCAATCT CCCTCCTTTG TCAAGTGAAT ACACTAAGGA TGTTGGATCC 
AAAAGTCAGT TAGTGACAGC TAATCCCAGC ATAATTCGGC AAAGGTTTCA GAACCTTCTG 
TGGTCCAGAA AGACATTTGT GGACAACATG AAAATTTATA ACCACAGTTA CATCTACATG 
CCTGCCTTTT CTATGAAGAC AGGAACAGAG CCATCTTTGA GGGTTTATTA TACACTGTCA 
GATGTTGGTG CCAATCAAAC AGTGCTGTTT GCCAACCCCA ACTTTCTGCG TAGCATTGGA 
AAGTTCTGGA AAAGTAGAGG AATCCATGCC AAGCGCCTGT CCACAGGACT TTTTCTGGTG 
AGCGCAGCTC TGGGTCTCTG TGAAGAGGTG GCCATCTATG GCTTCTGGCC CTTCTCTGTG 
AATATGCATG AGCAGCCCAT CAGCCACCAC TACTATGACA ACGTCTTACC CTTTTCTGGC 
TTCCATGCCA TGCCCGAGGA ATTTCTCCAA CTCTGGTATC TTCATAAAAT CGGTGCACTG 
AGAATGCAGC TGGACCCATG TGAAGATACC TCACTCCAGC CCACTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ST8SIA1 ( NM_001304450.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001291379.1 Homo sapiens ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (ST8SIA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu46170
Clone ID Related Accession (Same CDS sequence)	NM_001304450.1
Accession Version	NM_001304450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EU041716.1, AC007671.7 and BM929838.1. On Jan 27, 2015 this sequence version replaced XM_005253469.1. Transcript Variant: This variant (2) lacks an internal exon, which results in translation initiation at an alternate downstream AUG codon, compared to variant 1. The resulting isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EU041716.1, SRR1660805.15635.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGACGGGGA GTTTTTATAC TCATTCACCA TTGACAATTC AACTTACTCT CTCTTCCCAC 
AGATGCAATC TCCCTCCTTT GTCAAGTGAA TACACTAAGG ATGTTGGATC CAAAAGTCAG 
TTAGTGACAG CTAATCCCAG CATAATTCGG CAAAGGTTTC AGAACCTTCT GTGGTCCAGA 
AAGACATTTG TGGACAACAT GAAAATTTAT AACCACAGTT ACATCTACAT GCCTGCCTTT 
TCTATGAAGA CAGGAACAGA GCCATCTTTG AGGGTTTATT ATACACTGTC AGATGTTGGT 
GCCAATCAAA CAGTGCTGTT TGCCAACCCC AACTTTCTGC GTAGCATTGG AAAGTTCTGG 
AAAAGTAGAG GAATCCATGC CAAGCGCCTG TCCACAGGAC TTTTTCTGGT GAGCGCAGCT 
CTGGGTCTCT GTGAAGAGGT GGCCATCTAT GGCTTCTGGC CCTTCTCTGT GAATATGCAT 
GAGCAGCCCA TCAGCCACCA CTACTATGAC AACGTCTTAC CCTTTTCTGG CTTCCATGCC 
ATGCCCGAGG AATTTCTCCA ACTCTGGTAT CTTCATAAAA TCGGTGCACT GAGAATGCAG 
CTGGACCCAT GTGAAGATAC CTCACTCCAG CCCACTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001291379.1
CDS	802..1443
Translation	MTGSFYTHSPLTIQLTLSSHRCNLPPLSSEYTKDVGSKSQLVTANPSIIRQRFQNLLWSRKTFVDNMKIYNHSYIYMPAFSMKTGTEPSLRVYYTLSDVGANQTVLFANPNFLRSIGKFWKSRGIHAKRLSTGLFLVSAALGLCEEVAIYGFWPFSVNMHEQPISHHYYDNVLPFSGFHAMPEEFLQLWYLHKIGALRMQLDPCEDTSLQPTS

Target ORF information:

RefSeq Version	NM_001304450.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (ST8SIA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001304450.1

ORF Insert Sequence:

ATGACGGGGA GTTTTTATAC TCATTCACCA TTGACAATTC AACTTACTCT CTCTTCCCAC 
AGATGCAATC TCCCTCCTTT GTCAAGTGAA TACACTAAGG ATGTTGGATC CAAAAGTCAG 
TTAGTGACAG CTAATCCCAG CATAATTCGG CAAAGGTTTC AGAACCTTCT GTGGTCCAGA 
AAGACATTTG TGGACAACAT GAAAATTTAT AACCACAGTT ACATCTACAT GCCTGCCTTT 
TCTATGAAGA CAGGAACAGA GCCATCTTTG AGGGTTTATT ATACACTGTC AGATGTTGGT 
GCCAATCAAA CAGTGCTGTT TGCCAACCCC AACTTTCTGC GTAGCATTGG AAAGTTCTGG 
AAAAGTAGAG GAATCCATGC CAAGCGCCTG TCCACAGGAC TTTTTCTGGT GAGCGCAGCT 
CTGGGTCTCT GTGAAGAGGT GGCCATCTAT GGCTTCTGGC CCTTCTCTGT GAATATGCAT 
GAGCAGCCCA TCAGCCACCA CTACTATGAC AACGTCTTAC CCTTTTCTGG CTTCCATGCC 
ATGCCCGAGG AATTTCTCCA ACTCTGGTAT CTTCATAAAA TCGGTGCACT GAGAATGCAG 
CTGGACCCAT GTGAAGATAC CTCACTCCAG CCCACTTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification and Functional Characterization of ST3GAL5 and ST8SIA1 Variants in Patients with Thyroid-Associated Ophthalmopathy.
Yonsei medical journal58(6)1160-1169(2017 Nov)
Park HJ,Kim JH,Yoon JS,Choi YJ,Choi YH,Kook KH,Choi JH

MicroRNA-33a and let-7e inhibit human colorectal cancer progression by targeting ST8SIA1.
The international journal of biochemistry & cell biology9048-58(2017 Sep)
Shan Y,Liu Y,Zhao L,Liu B,Li Y,Jia L

Ganglioside glycosyltransferases are S-acylated at conserved cysteine residues involved in homodimerisation.
The Biochemical journal474(16)2803-2816(2017 08)
Chumpen Ramirez S,Ruggiero FM,Daniotti JL,Valdez Taubas J