St8sia1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	St8sia1
Entrez Gene ID	25280
Full Name	ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1
Synonyms	Siat8,Siat8a
General protein information	Preferred Names ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 Names alpha-N-acetylneuraminide alpha-2,8-sialyltransferase GD3 synthase ST8 alpha-N-acetylneuraminate alpha-2,8-sialyltransferase 1 Sialyltransferase 8 (alpha-N-acetylneuraminate: alpha-28-sialytransferase GD3 synthase) Sialyltransferase 8 A (alpha-N-acetylneuraminate: alpha-28-sialytransferase GD3 synthase) GenBank no: U53883 alpha-2, 8-sialytransferase alpha-2,8-sialyltransferase alpha-N-acetylneuraminate: alpha-2,8-sialytransferase sialyltransferase 8 (alpha-2 8-sialytransferase) A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q44
Summary	an alpha-2,8-sialyltransferase that catalyzes the biosynthesis of GT1a and GQ1b as well as GD3 [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012813.2	NP_036945.2	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase

Pathways from BioSystems

Homologies

Homo sapiens (human)	ST8SIA1	NP_003025.1
	ST8SIA1	XP_001099511.1
Bos taurus (cattle)	ST8SIA1	NP_001027470.1
Gallus gallus (chicken)	ST8SIA1	NP_990339.1
Canis lupus familiaris (dog)	ST8SIA1	XP_534865.2
Pan troglodytes (chimpanzee)	ST8SIA1	NP_001032380.1
Mus musculus (house mouse)	St8sia1	NP_035504.2
Rattus norvegicus (Norway rat)	St8sia1	NP_036945.2
Danio rerio (zebrafish)	LOC100333872	XP_002661434.1

Gene Ontology
Bibliography

Related articles in PubMed

Analysis of HSF4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses.
Fujimoto M, Oshima K, Shinkawa T, Wang BB, Inouye S, Hayashida N, Takii R, Nakai A
The Journal of biological chemistry283(44)29961-70(2008 Oct)

GD3 synthase gene found expressed in dental epithelium and shown to regulate cell proliferation.
Yamada A, Fukumoto E, Kamasaki Y, Ida-Yonemochi H, Saku T, Fujiwara T, Fukumoto S
Archives of oral biology50(4)393-9(2005 Apr)

Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility.
Kamimura Y, Furukawa K, Kittaka D, Nishio M, Hamamura K, Fukumoto S, Furukawa K
International journal of oncology26(2)337-44(2005 Feb)

Beta-amyloid-induced synthesis of the ganglioside GD3 is a requisite for cell cycle reactivation and apoptosis in neurons.
Copani A, Melchiorri D, Caricasole A, Martini F, Sale P, Carnevale R, Gradini R, Sortino MA, Lenti L, De Maria R, Nicoletti F
The Journal of neuroscience : the official journal of the Society for Neuroscience22(10)3963-8(2002 May)

The molecular cloning and expression of alpha 2,8-sialyltransferase (GD3 synthase) in a rat brain.
Watanabe Y, Nara K, Takahashi H, Nagai Y, Sanai Y
Journal of biochemistry120(5)1020-7(1996 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

alpha2,8-sialyltransferase has roles in cell proliferation and mobility
Title: Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility.

The following St8sia1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the St8sia1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42018	NM_012813.2 Latest version!	Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

St8sia1 ( NM_012813.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036945.2 Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), mRNA.

Price & Availability↑

CloneID	ORa42018
Clone ID Related Accession (Same CDS sequence)	NM_012813.2
Accession Version	NM_012813.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	alpha-N-acetylneuraminide alpha-2,8-sialyltransferase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB556962.1 and D45255.1. On Jul 27, 2012 this sequence version replaced NM_012813.1. ##Evidence-Data-START## Transcript exon combination :: U53883.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCCCCT GCGGGCGGGC CCTACATACA TCCAGAGGGG CCATGGCCAT GCTGGCGCGG 
AAATTCCCGC GTACCCGGCT ACCCGTGGGA GCCAGTGCCC TCTGTGTGGT GGTCCTCTGT 
TGGCTCTATG TCTTCCCTGT CTACCGGCTG CCCAACGAGA AGGAGATCGT GCAAGGAGTG 
CTGGCGCAGC GCACAGCTTG GAGGAGGAAC CAGACGTCAG CCAGGCTCTT CAGGAAACAA 
ATGGAAGACT GCTGCAACCC CGCCCATCTC TTTGCAATGA CGAAGGTGAA CTCCCCCATG 
GGGAAGAGCC TGTGGTATGA CGGGGAGTTT CTATACTCGC TCACCATCGA CAACTCCACG 
TACTCCCTCT TCCCCCAGGC AACCCCCTTC CAGCTGCCAT TGAAGAAATG TGCGGTGGTG 
GGAAACGGTG GGATTCTGAA GATGAGTGGC TGTGGCCGTC AAATAGATGA AGCAAATTTT 
GTCATGCGGT GTAACCTTCC TCCCTTGTCA AGTGAATACA CCAGAGATGT GGGTTCCAAG 
ACTCAGTTGG TGACAGCTAA CCCCAGCATA ATTCGCCAGA GATTTGAAAA CCTGCTGTGG 
TCCAGAAAGA AGTTTGTGGA CAACATGAAG ATCTATAACC ACAGTTACAT ATACATGCCT 
GCCTTTTCTA TGAAGACGGG AACAGAGCCG TCTCTCCGTG TGTACTACAC CCTGAAAGAC 
GCTGGAGCCA ATCAAACAGT GCTTTTTGCT AACCCCAACT TTCTTCGAAA CATTGGAAAG 
TTCTGGAAGG GTAGGGGGAT CCATGCCAAG CGCCTGTCTA CAGGACTCTT TTTGGTGAGT 
GCAGCCTTGG GCCTTTGTGA GGAGGTGTCC ATCTACGGCT TCTGGCCCTT CTCCGTGAAC 
ATGCAAGGGG AACCTATCAG CCACCATTAC TATGACAACG TCTTGCCCTT CTCAGGCTTC 
CATGCCATGC CTGAGGAGTT TCTTCAACTC TGGTATCTTC ATAAAATGGG TGCACTGAGG 
ATGCAACTGG ACCCATGTGA GGAGCCGTCG CCGCCACAGC CCACTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036945.2
CDS	301..1371
Translation	MSPCGRALHTSRGAMAMLARKFPRTRLPVGASALCVVVLCWLYVFPVYRLPNEKEIVQGVLAQRTAWRRNQTSARLFRKQMEDCCNPAHLFAMTKVNSPMGKSLWYDGEFLYSLTIDNSTYSLFPQATPFQLPLKKCAVVGNGGILKMSGCGRQIDEANFVMRCNLPPLSSEYTRDVGSKTQLVTANPSIIRQRFENLLWSRKKFVDNMKIYNHSYIYMPAFSMKTGTEPSLRVYYTLKDAGANQTVLFANPNFLRNIGKFWKGRGIHAKRLSTGLFLVSAALGLCEEVSIYGFWPFSVNMQGEPISHHYYDNVLPFSGFHAMPEEFLQLWYLHKMGALRMQLDPCEEPSPPQPTS

Target ORF information:

RefSeq Version	NM_012813.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 1 (St8sia1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012813.2

ORF Insert Sequence:

ATGAGCCCCT GCGGGCGGGC CCTACATACA TCCAGAGGGG CCATGGCCAT GCTGGCGCGG 
AAATTCCCGC GTACCCGGCT ACCCGTGGGA GCCAGTGCCC TCTGTGTGGT GGTCCTCTGT 
TGGCTCTATG TCTTCCCTGT CTACCGGCTG CCCAACGAGA AGGAGATCGT GCAAGGAGTG 
CTGGCGCAGC GCACAGCTTG GAGGAGGAAC CAGACGTCAG CCAGGCTCTT CAGGAAACAA 
ATGGAAGACT GCTGCAACCC CGCCCATCTC TTTGCAATGA CGAAGGTGAA CTCCCCCATG 
GGGAAGAGCC TGTGGTATGA CGGGGAGTTT CTATACTCGC TCACCATCGA CAACTCCACG 
TACTCCCTCT TCCCCCAGGC AACCCCCTTC CAGCTGCCAT TGAAGAAATG TGCGGTGGTG 
GGAAACGGTG GGATTCTGAA GATGAGTGGC TGTGGCCGTC AAATAGATGA AGCAAATTTT 
GTCATGCGGT GTAACCTTCC TCCCTTGTCA AGTGAATACA CCAGAGATGT GGGTTCCAAG 
ACTCAGTTGG TGACAGCTAA CCCCAGCATA ATTCGCCAGA GATTTGAAAA CCTGCTGTGG 
TCCAGAAAGA AGTTTGTGGA CAACATGAAG ATCTATAACC ACAGTTACAT ATACATGCCT 
GCCTTTTCTA TGAAGACGGG AACAGAGCCG TCTCTCCGTG TGTACTACAC CCTGAAAGAC 
GCTGGAGCCA ATCAAACAGT GCTTTTTGCT AACCCCAACT TTCTTCGAAA CATTGGAAAG 
TTCTGGAAGG GTAGGGGGAT CCATGCCAAG CGCCTGTCTA CAGGACTCTT TTTGGTGAGT 
GCAGCCTTGG GCCTTTGTGA GGAGGTGTCC ATCTACGGCT TCTGGCCCTT CTCCGTGAAC 
ATGCAAGGGG AACCTATCAG CCACCATTAC TATGACAACG TCTTGCCCTT CTCAGGCTTC 
CATGCCATGC CTGAGGAGTT TCTTCAACTC TGGTATCTTC ATAAAATGGG TGCACTGAGG 
ATGCAACTGG ACCCATGTGA GGAGCCGTCG CCGCCACAGC CCACTTCCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Analysis of HSF4 binding regions reveals its necessity for gene regulation during development and heat shock response in mouse lenses.
The Journal of biological chemistry283(44)29961-70(2008 Oct)
Fujimoto M,Oshima K,Shinkawa T,Wang BB,Inouye S,Hayashida N,Takii R,Nakai A

GD3 synthase gene found expressed in dental epithelium and shown to regulate cell proliferation.
Archives of oral biology50(4)393-9(2005 Apr)
Yamada A,Fukumoto E,Kamasaki Y,Ida-Yonemochi H,Saku T,Fujiwara T,Fukumoto S

Differential enhancing effects of alpha2,8-sialyltransferase on the cell proliferation and mobility.
International journal of oncology26(2)337-44(2005 Feb)
Kamimura Y,Furukawa K,Kittaka D,Nishio M,Hamamura K,Fukumoto S,Furukawa K

Beta-amyloid-induced synthesis of the ganglioside GD3 is a requisite for cell cycle reactivation and apoptosis in neurons.
The Journal of neuroscience : the official journal of the Society for Neuroscience22(10)3963-8(2002 May)
Copani A,Melchiorri D,Caricasole A,Martini F,Sale P,Carnevale R,Gradini R,Sortino MA,Lenti L,De Maria R,Nicoletti F

The molecular cloning and expression of alpha 2,8-sialyltransferase (GD3 synthase) in a rat brain.
Journal of biochemistry120(5)1020-7(1996 Nov)
Watanabe Y,Nara K,Takahashi H,Nagai Y,Sanai Y