gRNAs
The following SPATA31A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPATA31A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OHu15519 |
|
| Clone ID Related Accession (Same CDS sequence) | NM_001085452.2 | |
| Accession Version | NM_001085452.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4086bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-25 | |
| Organism | Homo sapiens(human) | |
| Product | spermatogenesis-associated protein 31A1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK302569.1 and BX005214.7. On Apr 11, 2014 this sequence version replaced NM_001085452.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK302569.1, BC146855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGCCCAGAG CTCAGTTGCT TGAAAGCAAC GCGCCTATTC ACATGGAGAA TCTTCCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001078921.2 |
| CDS | 21..4106 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001085452.2 |
1 | ATGCCCAGAG CTCAGTTGCT TGAAAGCAAC GCGCCTATTC ACATGGAGAA TCTTCCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu106807 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001085452.3 , NM_001085452.4 | |
| Accession Version | NM_001085452.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-02-27 | |
| Organism | Homo sapiens(human) | |
| Product | spermatogenesis-associated protein 31A1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005214.7 and AI023217.1. On Jul 21, 2018 this sequence version replaced NM_001085452.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation. ##Evidence-Data-START## Transcript exon combination :: AK302569.1, BC146855.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## | |
1 | ATGGAGAATC TTCCCTTTCC TTTAAAATTA CTTAGTGCCT CATCGCTAAA CGCCCCCAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001078921.3 |
| CDS | 63..4106 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001085452.3 |
1 | ATGGAGAATC TTCCCTTTCC TTTAAAATTA CTTAGTGCCT CATCGCTAAA CGCCCCCAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu106807 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001085452.3 , NM_001085452.4 | |
| Accession Version | NM_001085452.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4044bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-25 | |
| Organism | Homo sapiens(human) | |
| Product | spermatogenesis-associated protein 31A1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX005214.7 and AI023217.1. On May 17, 2019 this sequence version replaced NM_001085452.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation. ##Evidence-Data-START## Transcript exon combination :: AK302569.1, BC146855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000377647.6/ ENSP00000366875.5 RefSeq Select criteria :: based on expression ##RefSeq-Attributes-END## | |
1 | ATGGAGAATC TTCCCTTTCC TTTAAAATTA CTTAGTGCCT CATCGCTAAA CGCCCCCAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001078921.3 |
| CDS | 63..4106 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001085452.4 |
1 | ATGGAGAATC TTCCCTTTCC TTTAAAATTA CTTAGTGCCT CATCGCTAAA CGCCCCCAGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu99987 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017030503.1 | |
| Accession Version | XM_017030503.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-05 | |
| Organism | Homo sapiens(human) | |
| Product | LOW QUALITY PROTEIN: spermatogenesis-associated protein 31A1 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_018920.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END## | |
1 | ATGCCCAGAG CTCAGTTGCT TGAAAGCAAC GCGCCTATTC ACATGGAGAA TCTTCCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_016885992.1 |
| CDS | 23..3403 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017030503.1 |
1 | ATGCCCAGAG CTCAGTTGCT TGAAAGCAAC GCGCCTATTC ACATGGAGAA TCTTCCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu80619 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011518006.2 , XM_011518006.1 | |
| Accession Version | XM_011518006.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-05 | |
| Organism | Homo sapiens(human) | |
| Product | spermatogenesis-associated protein 31A1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011518006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCCAGAG CTCAGTTGCT TGAAAGCAAC GCGCCTATTC ACATGGAGAA TCTTCCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011516308.1 |
| CDS | 23..4144 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_011518006.2 |
1 | ATGCCCAGAG CTCAGTTGCT TGAAAGCAAC GCGCCTATTC ACATGGAGAA TCTTCCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OHu80619 | |
| Clone ID Related Accession (Same CDS sequence) | XM_011518006.2 , XM_011518006.1 | |
| Accession Version | XM_011518006.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4122bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-05 | |
| Organism | Homo sapiens(human) | |
| Product | spermatogenesis-associated protein 31A1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCCCAGAG CTCAGTTGCT TGAAAGCAAC GCGCCTATTC ACATGGAGAA TCTTCCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_011516308.1 |
| CDS | 23..4144 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_011518006.1 |
1 | ATGCCCAGAG CTCAGTTGCT TGAAAGCAAC GCGCCTATTC ACATGGAGAA TCTTCCCTTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The testis-specific VAD1.3/AEP1 interacts with ?-actin and syntaxin 1 and directs peri-nuclear/Golgi expression with bipartite nucleus localization (BNL) sequence. |
