Spata31 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spata31
Entrez Gene ID	78124
Full Name	spermatogenesis associated 31
Synonyms	4930458L03Rik,Aep1,Fam75a,Spata31a,Vad1.3
General protein information	Preferred Names spermatogenesis associated 31 Names spermatogenesis-associated protein 31 acrosome expressed protein 1 acrosome-specific protein VAD1.3 family with sequence similarity 75, member A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 13 Map Location: 13\|13 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_030047.2	NP_084323.2	spermatogenesis-associated protein 31

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC749578	XP_001169917.2
Homo sapiens (human)	SPATA31A6	NP_001138668.1
	LOC719765	XP_001114434.2
Homo sapiens (human)	SPATA31A3	NP_001076593.1
Homo sapiens (human)	SPATA31A7	NP_056482.2
Homo sapiens (human)	SPATA31A1	NP_001078921.1
Pan troglodytes (chimpanzee)	LOC749550	XP_001169768.2
Bos taurus (cattle)	SPATA31A3	XP_005210502.1
Rattus norvegicus (Norway rat)	Spata31a5	NP_001008360.2
Homo sapiens (human)	SPATA31A5	NP_001107013.1
Canis lupus familiaris (dog)	SPATA31A3	XP_005615835.1
Mus musculus (house mouse)	Spata31	NP_084323.2

Gene Ontology
Bibliography

Related articles in PubMed

Targeted disruption of the spermatid-specific gene Spata31 causes male infertility.
Wu YY, Yang Y, Xu YD, Yu HL
Molecular reproduction and development82(6)432-40(2015 Jun)

The testis-specific VAD1.3/AEP1 interacts with ?-actin and syntaxin 1 and directs peri-nuclear/Golgi expression with bipartite nucleus localization (BNL) sequence.
Zuo Y, Gao J, Yeung WS, Lee KF
Biochemical and biophysical research communications401(2)275-80(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Targeted disruption of the spermatid-specific gene Spata31 causes male infertility.
Title: Targeted disruption of the spermatid-specific gene Spata31 causes male infertility.

VAD1.3 interacts with beta-actin and syntaxin 1 in vitro.
Title: The testis-specific VAD1.3/AEP1 interacts with ?-actin and syntaxin 1 and directs peri-nuclear/Golgi expression with bipartite nucleus localization (BNL) sequence.

The following Spata31 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spata31 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu07849	NM_030047.2 Latest version!	Mus musculus spermatogenesis associated 31 (Spata31), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spata31 ( NM_030047.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084323.2 Mus musculus spermatogenesis associated 31 (Spata31), mRNA.

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CloneID	OMu07849
Clone ID Related Accession (Same CDS sequence)	NM_030047.2
Accession Version	NM_030047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	spermatogenesis-associated protein 31
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK133249.1 and AC159190.2. On Dec 19, 2009 this sequence version replaced NM_030047.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY842249.1, AK133249.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAGCC TTCCCTCTCT TCTGGAAAGC ATTTACACCA CATGGCTGAG CCTCAGCTCC 
ACTATATGGG CAATGGACAT GATCCTTGCC TTTGTGTGTG GACTGGGGCT CTACCACCTG 
TTACTTCCTT TTCTTGAGAG TCATCTGTCT TCACCTCCAT CAAATATAAA GTTCACCAGG 
AAGCCTCAGA TACAGATGAC CTGGCAGAGC CAGTTCAAGA AAAAGTTTAG GAATCATTGT 
AGGAATGATG CAAAAGCTTG GGGAGAATGC CTGAAAAAGC TGAAAGAAAA AGAAAAGGAT 
AAATTATTTC TAGAAGAAAT GAGCCCAGGA CACCATTTGA ACTCTTTAGG GAATATATTT 
AACTCATCAA GTGCTAAACA AGACAGCACT ACCCTGTCAC CCTTTTGGAA CCTGAAAGAA 
AAATCAGAAG AGCAGGTGGC CACTCAGAAA CTATCTTATC CCAAGATCTC AGAAGACCAT 
TTTCAACAGA AATGTGACCA GCTTTTCTGG GGTCTCCCTT CTCTCCACAG TGAGTCTCTG 
GTAGCTGCCG CCTGGATCCC TCAGACAACG TCAACTCTCC CTTCTCCCTT TTTCTTATTC 
AATGTCATCT CAAGTGTTTA TCCAATTCAA CTGCAGGATA AAATGTCACC AATGCTTCCC 
CATACACATC CTCTGTCCTA TCTGGACCTC CAATCTCCAC ACTTAATTCT GTCTCCACTT 
GAATTCCAGA CCCCAGCTTT GAATCTCCCA GTCCTACTAC CCTCTTCTCT ACCCTATAAT 
AGTGATTTTG GTACATCTTA TTCTCAATCA CAAAGTAAGC CACAATACCT CCCAACTGAA 
ATAAAATACA ATAAAAAACC CTCACTGGCA AAACAAATAG AAAATAGATC AACTTTACCC 
TTGATGGTCC AGAAACCTCA AGAAGCCTAT GACATCTTGG CCCCCAACCC TTCCCAAGAC 
TGGGTAGTCT CCATCCTTCC TGATAATTTT CCCATCTGCC GTGAGCTCCG GGAGAAACTG 
GAGCAACACA TTCAAAAGTG GCTCATTCAA CACCAATGGA ATTTTCCCCA TAAGATCCAA 
GTGTCTGAGA AAATGAAGGA GCTTCAGAAC ACAGTAATAG GAAATTGTCA AACCAGAGAC 
AAGCCTGGCA CCTTACAAGC CCTTGGTGAA CACAGCAATG AGGGCCAGAA GAGAAAATTC 
CAGCTAGAAA AGGAATCTGG CAAGAATCTG GGACCTATTC TAGGAAAGAT CTCAAAAGAT 
CCAATCAGGG GCTTGGAAAA AACTACAGCA ATCAAAGATT TAGAAAACAA CTTGAAAGCT 
CATTTGGGTA CAAAGTCAGG GCAGATAGAT CAAGGTCTGA CCCCTCTAAG TATGCGACAA 
TCCTGGCTTG CTGTGGATGA TAGTTTTTAC GAGATGGAAA ACAATTTAAC ATCCTTAAAG 
AGTTCAGCAA AGTCCATGTG CTCTTCAGAG AAGCTTGCCT TTCTCAAACC AGAAACTCGA 
CAGGCTCTGG AGGCACATAT TGTAAGGTTT TGGGCAAAGC ACAGGTGGAG TCTACCCCTT 
AAGATACTCA AGCCCGTAAA ACTCTTTCAG TTAAGTATTG AGTCCTTGCC TGTTGTACTC 
TGTGCCCAGA CCTCCTCAAC CACTTCTGTA CATAGGACCA GATCAGCAGC TGAAGTAGTC 
AGATTCCTAG GAAAACCATG CTTGAGACAG ATGATAATTG AGGATTCTTC TCCATCACCA 
CAGAATCTTC TCCTTGTCTC ATCTCCTTCC TGTAAGAGGG CCAGGAGAAG GCTTCCCTTT 
GGTGTTGACC ATGAGCCTTC TACAGCCCTG CCAACCAAAC CAGAGTGTGG GCATATTTCA 
GAAGATCTCA CTTATAATTT CATGAACACA ACCTCTCAGA CTAGGACTAT CTTCAAGAAA 
GAGATAGAGA CTCGAGAAGT CGTTTTACTG CCTAGAAGGA CGAGTGACCA AAATTTGGAG 
GCATACAAAC ATCAGGAAAA AGTTGTTAGT GAGTTTCCAC ATAATGTGGA GACAGAATTA 
GCAGGCCAGC CACAAATCTA TACCACTACT GTGCTCCCTC CAAAACGTTC CAGGAGCATG 
CCTCTTCCTG TAGACACTTT GACTTCACAT GTATTAGGTG ACATTGTGGT GGCAGACATG 
GACAATAGCC TGGTGCAGCA GAGACCCAGT ACTCCAAAGC ATCTAGTCTC ACAGAAGAGC 
CAAATCAAGA TGTTGGCCCC TACTTATCAA AGTGAGGGCA CTAAGAGACA GAGTGAAATA 
AAGTATGAAG ACAGACCTAA GCTCACCCCA GTCACAGAGA AAAAAGCCAA CTTTGGCAGC 
CAATACTACC AGACCCTTCC AAAAATCGCA CAAGTGCTTC CAGGAAGGCC CCCCCAAAGA 
CATTTAGGTC GCTTTCTACA GTGGATTCAT CCTAAGAAAA CAATCAAAGG GCATGAATTT 
CATCCCCTAA AAGGTAATCC TACAGCAGCC ATTGTCCAGA ACCAACGAAG GCAAGTGAGA 
AAAATACCAT ATATGGACAA CAATGTGACT GAGGCCCAGG AACTTATGAC AACTGTTGGA 
CAGATGTTAG AAAAGAAAAT GATGCTGCAA CATCAACCCT ATGCTTCAAA ATTCAACCAG 
CACAGGGAAG TTCCTCCAGC CCCCATGTCT CGGTTTTCCC ATGGCCACAT GCCAGTCTCC 
TACTTACAGC AAAGGAGAGC ACCCAGTTAC CCTGGCAGTT GCTCATGTCA GAGGTGTTCT 
GTACAGAACA GGCACATCAG AAACCAACTG CCTCAGAGAA GTGTACGCTT TAGCAAGAAA 
CCACAGAACC CACGGAACCC CAGCCACAAG AAACCACAGA ACCCACGGAA CCCCAGCCGC 
TTGTCCCGTA ACGCAGCTTT GAACCTAGTG AATTCTCAGA ACAGAACAAT AGTGCCAGGA 
AATTCAAATC ACCATCTTTA TTGTCCTAGG CACTGTGCCC TCCAGAGAGA TGTCTGTAGA 
GAACTAGGAC ATTCCTCTGT AGTTTTTCCT AATAGGAAAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084323.2
CDS	78..3122
Translation	MESLPSLLESIYTTWLSLSSTIWAMDMILAFVCGLGLYHLLLPFLESHLSSPPSNIKFTRKPQIQMTWQSQFKKKFRNHCRNDAKAWGECLKKLKEKEKDKLFLEEMSPGHHLNSLGNIFNSSSAKQDSTTLSPFWNLKEKSEEQVATQKLSYPKISEDHFQQKCDQLFWGLPSLHSESLVAAAWIPQTTSTLPSPFFLFNVISSVYPIQLQDKMSPMLPHTHPLSYLDLQSPHLILSPLEFQTPALNLPVLLPSSLPYNSDFGTSYSQSQSKPQYLPTEIKYNKKPSLAKQIENRSTLPLMVQKPQEAYDILAPNPSQDWVVSILPDNFPICRELREKLEQHIQKWLIQHQWNFPHKIQVSEKMKELQNTVIGNCQTRDKPGTLQALGEHSNEGQKRKFQLEKESGKNLGPILGKISKDPIRGLEKTTAIKDLENNLKAHLGTKSGQIDQGLTPLSMRQSWLAVDDSFYEMENNLTSLKSSAKSMCSSEKLAFLKPETRQALEAHIVRFWAKHRWSLPLKILKPVKLFQLSIESLPVVLCAQTSSTTSVHRTRSAAEVVRFLGKPCLRQMIIEDSSPSPQNLLLVSSPSCKRARRRLPFGVDHEPSTALPTKPECGHISEDLTYNFMNTTSQTRTIFKKEIETREVVLLPRRTSDQNLEAYKHQEKVVSEFPHNVETELAGQPQIYTTTVLPPKRSRSMPLPVDTLTSHVLGDIVVADMDNSLVQQRPSTPKHLVSQKSQIKMLAPTYQSEGTKRQSEIKYEDRPKLTPVTEKKANFGSQYYQTLPKIAQVLPGRPPQRHLGRFLQWIHPKKTIKGHEFHPLKGNPTAAIVQNQRRQVRKIPYMDNNVTEAQELMTTVGQMLEKKMMLQHQPYASKFNQHREVPPAPMSRFSHGHMPVSYLQQRRAPSYPGSCSCQRCSVQNRHIRNQLPQRSVRFSKKPQNPRNPSHKKPQNPRNPSRLSRNAALNLVNSQNRTIVPGNSNHHLYCPRHCALQRDVCRELGHSSVVFPNRKT

Target ORF information:

RefSeq Version	NM_030047.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus spermatogenesis associated 31 (Spata31), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030047.2

ORF Insert Sequence:

ATGGAGAGCC TTCCCTCTCT TCTGGAAAGC ATTTACACCA CATGGCTGAG CCTCAGCTCC 
ACTATATGGG CAATGGACAT GATCCTTGCC TTTGTGTGTG GACTGGGGCT CTACCACCTG 
TTACTTCCTT TTCTTGAGAG TCATCTGTCT TCACCTCCAT CAAATATAAA GTTCACCAGG 
AAGCCTCAGA TACAGATGAC CTGGCAGAGC CAGTTCAAGA AAAAGTTTAG GAATCATTGT 
AGGAATGATG CAAAAGCTTG GGGAGAATGC CTGAAAAAGC TGAAAGAAAA AGAAAAGGAT 
AAATTATTTC TAGAAGAAAT GAGCCCAGGA CACCATTTGA ACTCTTTAGG GAATATATTT 
AACTCATCAA GTGCTAAACA AGACAGCACT ACCCTGTCAC CCTTTTGGAA CCTGAAAGAA 
AAATCAGAAG AGCAGGTGGC CACTCAGAAA CTATCTTATC CCAAGATCTC AGAAGACCAT 
TTTCAACAGA AATGTGACCA GCTTTTCTGG GGTCTCCCTT CTCTCCACAG TGAGTCTCTG 
GTAGCTGCCG CCTGGATCCC TCAGACAACG TCAACTCTCC CTTCTCCCTT TTTCTTATTC 
AATGTCATCT CAAGTGTTTA TCCAATTCAA CTGCAGGATA AAATGTCACC AATGCTTCCC 
CATACACATC CTCTGTCCTA TCTGGACCTC CAATCTCCAC ACTTAATTCT GTCTCCACTT 
GAATTCCAGA CCCCAGCTTT GAATCTCCCA GTCCTACTAC CCTCTTCTCT ACCCTATAAT 
AGTGATTTTG GTACATCTTA TTCTCAATCA CAAAGTAAGC CACAATACCT CCCAACTGAA 
ATAAAATACA ATAAAAAACC CTCACTGGCA AAACAAATAG AAAATAGATC AACTTTACCC 
TTGATGGTCC AGAAACCTCA AGAAGCCTAT GACATCTTGG CCCCCAACCC TTCCCAAGAC 
TGGGTAGTCT CCATCCTTCC TGATAATTTT CCCATCTGCC GTGAGCTCCG GGAGAAACTG 
GAGCAACACA TTCAAAAGTG GCTCATTCAA CACCAATGGA ATTTTCCCCA TAAGATCCAA 
GTGTCTGAGA AAATGAAGGA GCTTCAGAAC ACAGTAATAG GAAATTGTCA AACCAGAGAC 
AAGCCTGGCA CCTTACAAGC CCTTGGTGAA CACAGCAATG AGGGCCAGAA GAGAAAATTC 
CAGCTAGAAA AGGAATCTGG CAAGAATCTG GGACCTATTC TAGGAAAGAT CTCAAAAGAT 
CCAATCAGGG GCTTGGAAAA AACTACAGCA ATCAAAGATT TAGAAAACAA CTTGAAAGCT 
CATTTGGGTA CAAAGTCAGG GCAGATAGAT CAAGGTCTGA CCCCTCTAAG TATGCGACAA 
TCCTGGCTTG CTGTGGATGA TAGTTTTTAC GAGATGGAAA ACAATTTAAC ATCCTTAAAG 
AGTTCAGCAA AGTCCATGTG CTCTTCAGAG AAGCTTGCCT TTCTCAAACC AGAAACTCGA 
CAGGCTCTGG AGGCACATAT TGTAAGGTTT TGGGCAAAGC ACAGGTGGAG TCTACCCCTT 
AAGATACTCA AGCCCGTAAA ACTCTTTCAG TTAAGTATTG AGTCCTTGCC TGTTGTACTC 
TGTGCCCAGA CCTCCTCAAC CACTTCTGTA CATAGGACCA GATCAGCAGC TGAAGTAGTC 
AGATTCCTAG GAAAACCATG CTTGAGACAG ATGATAATTG AGGATTCTTC TCCATCACCA 
CAGAATCTTC TCCTTGTCTC ATCTCCTTCC TGTAAGAGGG CCAGGAGAAG GCTTCCCTTT 
GGTGTTGACC ATGAGCCTTC TACAGCCCTG CCAACCAAAC CAGAGTGTGG GCATATTTCA 
GAAGATCTCA CTTATAATTT CATGAACACA ACCTCTCAGA CTAGGACTAT CTTCAAGAAA 
GAGATAGAGA CTCGAGAAGT CGTTTTACTG CCTAGAAGGA CGAGTGACCA AAATTTGGAG 
GCATACAAAC ATCAGGAAAA AGTTGTTAGT GAGTTTCCAC ATAATGTGGA GACAGAATTA 
GCAGGCCAGC CACAAATCTA TACCACTACT GTGCTCCCTC CAAAACGTTC CAGGAGCATG 
CCTCTTCCTG TAGACACTTT GACTTCACAT GTATTAGGTG ACATTGTGGT GGCAGACATG 
GACAATAGCC TGGTGCAGCA GAGACCCAGT ACTCCAAAGC ATCTAGTCTC ACAGAAGAGC 
CAAATCAAGA TGTTGGCCCC TACTTATCAA AGTGAGGGCA CTAAGAGACA GAGTGAAATA 
AAGTATGAAG ACAGACCTAA GCTCACCCCA GTCACAGAGA AAAAAGCCAA CTTTGGCAGC 
CAATACTACC AGACCCTTCC AAAAATCGCA CAAGTGCTTC CAGGAAGGCC CCCCCAAAGA 
CATTTAGGTC GCTTTCTACA GTGGATTCAT CCTAAGAAAA CAATCAAAGG GCATGAATTT 
CATCCCCTAA AAGGTAATCC TACAGCAGCC ATTGTCCAGA ACCAACGAAG GCAAGTGAGA 
AAAATACCAT ATATGGACAA CAATGTGACT GAGGCCCAGG AACTTATGAC AACTGTTGGA 
CAGATGTTAG AAAAGAAAAT GATGCTGCAA CATCAACCCT ATGCTTCAAA ATTCAACCAG 
CACAGGGAAG TTCCTCCAGC CCCCATGTCT CGGTTTTCCC ATGGCCACAT GCCAGTCTCC 
TACTTACAGC AAAGGAGAGC ACCCAGTTAC CCTGGCAGTT GCTCATGTCA GAGGTGTTCT 
GTACAGAACA GGCACATCAG AAACCAACTG CCTCAGAGAA GTGTACGCTT TAGCAAGAAA 
CCACAGAACC CACGGAACCC CAGCCACAAG AAACCACAGA ACCCACGGAA CCCCAGCCGC 
TTGTCCCGTA ACGCAGCTTT GAACCTAGTG AATTCTCAGA ACAGAACAAT AGTGCCAGGA 
AATTCAAATC ACCATCTTTA TTGTCCTAGG CACTGTGCCC TCCAGAGAGA TGTCTGTAGA 
GAACTAGGAC ATTCCTCTGT AGTTTTTCCT AATAGGAAAA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeted disruption of the spermatid-specific gene Spata31 causes male infertility.
Molecular reproduction and development82(6)432-40(2015 Jun)
Wu YY,Yang Y,Xu YD,Yu HL

The testis-specific VAD1.3/AEP1 interacts with ?-actin and syntaxin 1 and directs peri-nuclear/Golgi expression with bipartite nucleus localization (BNL) sequence.
Biochemical and biophysical research communications401(2)275-80(2010 Oct)
Zuo Y,Gao J,Yeung WS,Lee KF

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Spata31 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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