TPSB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TPSB2
Entrez Gene ID	64499
Full Name	tryptase beta 2 (gene/pseudogene)
Synonyms	TPS2,tryptaseB,tryptaseC
General protein information	Preferred Names tryptase beta 2 (gene/pseudogene) Names tryptase beta-2 mast cell tryptase beta II mast cell tryptase beta III testicular tissue protein Li 163
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, beta II and beta III. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha-tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008].
Disorder MIM:	191081

mRNA and Protein(s)

mRNA	Protein	Name
NM_024164.5	NP_077078.5	tryptase beta-2 preproprotein
NM_024164.6	NP_077078.5	tryptase beta-2 preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LOC786948	XP_003587843.1
	TPSB2	XP_001088175.1
Canis lupus familiaris (dog)	LOC100049001	NP_001091024.1
Mus musculus (house mouse)	Tpsb2	NP_034911.3
Homo sapiens (human)	TPSB2	NP_077078.5
Homo sapiens (human)	TPSAB1	NP_003285.2
Bos taurus (cattle)	TPSB1	XP_005224652.1
Pan troglodytes (chimpanzee)	TPSB2	NP_001129130.1
Rattus norvegicus (Norway rat)	Tpsb2	NP_062053.2

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Allosteric control of ?II-tryptase by a redox active disulfide bond.
Cook KM, McNeil HP, Hogg PJ
The Journal of biological chemistry288(48)34920-9(2013 Nov)

Identification and analysis of unitary pseudogenes: historic and contemporary gene losses in humans and other primates.
Zhang ZD, Frankish A, Hunt T, Harrow J, Gerstein M
Genome biology11(3)R26(2010)

Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutations.
Trivedi NN, Tamraz B, Chu C, Kwok PY, Caughey GH
The Journal of allergy and clinical immunology124(5)1099-105.e1-4(2009 Nov)

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening.
Wu C, Ma MH, Brown KR, Geisler M, Li L, Tzeng E, Jia CY, Jurisica I, Li SS
Proteomics7(11)1775-85(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These observations indicate that betaII-tryptase activity is post-translationally regulated by an allosteric disulfide bond.
Title: Allosteric control of ?II-tryptase by a redox active disulfide bond.

Strong linkage of TPSAB1 and TPSB2 and pairing of deficiency alleles with functional alleles in observed haplotypes protect human subjects from "knockout" genomes and indeed from inheritance of fewer than 2 active alleles.
Title: Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutations.

The disease severity and plasma tryptase levels were not affected by the number of alpha or beta tryptase alleles in mastocytosis patients.
Title: Tryptase haplotype in mastocytosis: relationship to disease variant and diagnostic utility of total tryptase levels.

Results indicate a key role for heparin in the activation of human betaI- and beta2-tryptase.
Title: Structural requirements and mechanism for heparin-dependent activation and tetramerization of human betaI- and betaII-tryptase.

The following TPSB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPSB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18064	NM_024164.6 Latest version!	Homo sapiens tryptase beta 2 (gene/pseudogene) (TPSB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu18064	NM_024164.5	Homo sapiens tryptase beta 2 (gene/pseudogene) (TPSB2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPSB2 ( NM_024164.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_077078.5 Homo sapiens tryptase beta 2 (gene/pseudogene) (TPSB2), mRNA.

Price & Availability↑

CloneID	OHu18064
Clone ID Related Accession (Same CDS sequence)	NM_024164.5 , NM_024164.6
Accession Version	NM_024164.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	tryptase beta-2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF099143.1. This sequence is a reference standard in the RefSeqGene project. On Jul 1, 2005 this sequence version replaced NM_024164.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028059.1, BM919268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## polymorphic pseudogene :: PMID: 20210993 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAATC TGCTGCTGCT GGCGCTGCCC GTCCTGGCGA GCCGCGCCTA CGCGGCCCCT 
GCCCCAGGCC AGGCCCTGCA GCGAGTGGGC ATCGTTGGGG GTCAGGAGGC CCCCAGGAGC 
AAGTGGCCCT GGCAGGTGAG CCTGAGAGTC CGCGACCGAT ACTGGATGCA CTTCTGCGGG 
GGCTCCCTCA TCCACCCCCA GTGGGTGCTG ACCGCAGCGC ACTGCGTGGG ACCGGACGTC 
AAGGATCTGG CCGCCCTCAG GGTGCAACTG CGGGAGCAGC ACCTCTACTA CCAGGACCAG 
CTGCTGCCGG TCAGCAGGAT CATCGTGCAC CCACAGTTCT ACACCGCCCA GATCGGAGCG 
GACATCGCCC TGCTGGAGCT GGAGGAGCCG GTGAACGTCT CCAGCCACGT CCACACGGTC 
ACCCTGCCCC CTGCCTCAGA GACCTTCCCC CCGGGGATGC CGTGCTGGGT CACTGGCTGG 
GGCGATGTGG ACAATGATGA GCGCCTCCCA CCGCCATTTC CTCTGAAGCA GGTGAAGGTC 
CCCATAATGG AAAACCACAT TTGTGACGCA AAATACCACC TTGGCGCCTA CACGGGAGAC 
GACGTCCGCA TCGTCCGTGA CGACATGCTG TGTGCCGGGA ACACCCGGAG GGACTCATGC 
CAGGGCGACT CCGGAGGGCC CCTGGTGTGC AAGGTGAATG GCACCTGGCT GCAGGCGGGC 
GTGGTCAGCT GGGGCGAGGG CTGTGCCCAG CCCAACCGGC CTGGCATCTA CACCCGTGTC 
ACCTACTACT TGGACTGGAT CCACCACTAT GTCCCCAAAA AGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077078.5
CDS	24..851
Translation	MLNLLLLALPVLASRAYAAPAPGQALQRVGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYTAQIGADIALLELEEPVNVSSHVHTVTLPPASETFPPGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP

Target ORF information:

RefSeq Version	NM_024164.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tryptase beta 2 (gene/pseudogene) (TPSB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024164.5

ORF Insert Sequence:

ATGCTGAATC TGCTGCTGCT GGCGCTGCCC GTCCTGGCGA GCCGCGCCTA CGCGGCCCCT 
GCCCCAGGCC AGGCCCTGCA GCGAGTGGGC ATCGTTGGGG GTCAGGAGGC CCCCAGGAGC 
AAGTGGCCCT GGCAGGTGAG CCTGAGAGTC CGCGACCGAT ACTGGATGCA CTTCTGCGGG 
GGCTCCCTCA TCCACCCCCA GTGGGTGCTG ACCGCAGCGC ACTGCGTGGG ACCGGACGTC 
AAGGATCTGG CCGCCCTCAG GGTGCAACTG CGGGAGCAGC ACCTCTACTA CCAGGACCAG 
CTGCTGCCGG TCAGCAGGAT CATCGTGCAC CCACAGTTCT ACACCGCCCA GATCGGAGCG 
GACATCGCCC TGCTGGAGCT GGAGGAGCCG GTGAACGTCT CCAGCCACGT CCACACGGTC 
ACCCTGCCCC CTGCCTCAGA GACCTTCCCC CCGGGGATGC CGTGCTGGGT CACTGGCTGG 
GGCGATGTGG ACAATGATGA GCGCCTCCCA CCGCCATTTC CTCTGAAGCA GGTGAAGGTC 
CCCATAATGG AAAACCACAT TTGTGACGCA AAATACCACC TTGGCGCCTA CACGGGAGAC 
GACGTCCGCA TCGTCCGTGA CGACATGCTG TGTGCCGGGA ACACCCGGAG GGACTCATGC 
CAGGGCGACT CCGGAGGGCC CCTGGTGTGC AAGGTGAATG GCACCTGGCT GCAGGCGGGC 
GTGGTCAGCT GGGGCGAGGG CTGTGCCCAG CCCAACCGGC CTGGCATCTA CACCCGTGTC 
ACCTACTACT TGGACTGGAT CCACCACTAT GTCCCCAAAA AGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPSB2 ( NM_024164.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_077078.5 Homo sapiens tryptase beta 2 (gene/pseudogene) (TPSB2), mRNA.

Price & Availability↑

CloneID	OHu18064
Clone ID Related Accession (Same CDS sequence)	NM_024164.5 , NM_024164.6
Accession Version	NM_024164.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	tryptase beta-2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC120498.2 and AMYH02031223.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_024164.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DRR138524.388092.1, BC028059.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000606293.5/ ENSP00000482743.1 polymorphic pseudogene :: PMID: 20210993 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGAATC TGCTGCTGCT GGCGCTGCCC GTCCTGGCGA GCCGCGCCTA CGCGGCCCCT 
GCCCCAGGCC AGGCCCTGCA GCGAGTGGGC ATCGTTGGGG GTCAGGAGGC CCCCAGGAGC 
AAGTGGCCCT GGCAGGTGAG CCTGAGAGTC CGCGACCGAT ACTGGATGCA CTTCTGCGGG 
GGCTCCCTCA TCCACCCCCA GTGGGTGCTG ACCGCAGCGC ACTGCGTGGG ACCGGACGTC 
AAGGATCTGG CCGCCCTCAG GGTGCAACTG CGGGAGCAGC ACCTCTACTA CCAGGACCAG 
CTGCTGCCGG TCAGCAGGAT CATCGTGCAC CCACAGTTCT ACACCGCCCA GATCGGAGCG 
GACATCGCCC TGCTGGAGCT GGAGGAGCCG GTGAACGTCT CCAGCCACGT CCACACGGTC 
ACCCTGCCCC CTGCCTCAGA GACCTTCCCC CCGGGGATGC CGTGCTGGGT CACTGGCTGG 
GGCGATGTGG ACAATGATGA GCGCCTCCCA CCGCCATTTC CTCTGAAGCA GGTGAAGGTC 
CCCATAATGG AAAACCACAT TTGTGACGCA AAATACCACC TTGGCGCCTA CACGGGAGAC 
GACGTCCGCA TCGTCCGTGA CGACATGCTG TGTGCCGGGA ACACCCGGAG GGACTCATGC 
CAGGGCGACT CCGGAGGGCC CCTGGTGTGC AAGGTGAATG GCACCTGGCT GCAGGCGGGC 
GTGGTCAGCT GGGGCGAGGG CTGTGCCCAG CCCAACCGGC CTGGCATCTA CACCCGTGTC 
ACCTACTACT TGGACTGGAT CCACCACTAT GTCCCCAAAA AGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077078.5
CDS	24..851
Translation	MLNLLLLALPVLASRAYAAPAPGQALQRVGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCVGPDVKDLAALRVQLREQHLYYQDQLLPVSRIIVHPQFYTAQIGADIALLELEEPVNVSSHVHTVTLPPASETFPPGMPCWVTGWGDVDNDERLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIVRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWGEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP

Target ORF information:

RefSeq Version	NM_024164.6
Organism	Homo sapiens(human)
Definition	Homo sapiens tryptase beta 2 (gene/pseudogene) (TPSB2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024164.6

ORF Insert Sequence:

ATGCTGAATC TGCTGCTGCT GGCGCTGCCC GTCCTGGCGA GCCGCGCCTA CGCGGCCCCT 
GCCCCAGGCC AGGCCCTGCA GCGAGTGGGC ATCGTTGGGG GTCAGGAGGC CCCCAGGAGC 
AAGTGGCCCT GGCAGGTGAG CCTGAGAGTC CGCGACCGAT ACTGGATGCA CTTCTGCGGG 
GGCTCCCTCA TCCACCCCCA GTGGGTGCTG ACCGCAGCGC ACTGCGTGGG ACCGGACGTC 
AAGGATCTGG CCGCCCTCAG GGTGCAACTG CGGGAGCAGC ACCTCTACTA CCAGGACCAG 
CTGCTGCCGG TCAGCAGGAT CATCGTGCAC CCACAGTTCT ACACCGCCCA GATCGGAGCG 
GACATCGCCC TGCTGGAGCT GGAGGAGCCG GTGAACGTCT CCAGCCACGT CCACACGGTC 
ACCCTGCCCC CTGCCTCAGA GACCTTCCCC CCGGGGATGC CGTGCTGGGT CACTGGCTGG 
GGCGATGTGG ACAATGATGA GCGCCTCCCA CCGCCATTTC CTCTGAAGCA GGTGAAGGTC 
CCCATAATGG AAAACCACAT TTGTGACGCA AAATACCACC TTGGCGCCTA CACGGGAGAC 
GACGTCCGCA TCGTCCGTGA CGACATGCTG TGTGCCGGGA ACACCCGGAG GGACTCATGC 
CAGGGCGACT CCGGAGGGCC CCTGGTGTGC AAGGTGAATG GCACCTGGCT GCAGGCGGGC 
GTGGTCAGCT GGGGCGAGGG CTGTGCCCAG CCCAACCGGC CTGGCATCTA CACCCGTGTC 
ACCTACTACT TGGACTGGAT CCACCACTAT GTCCCCAAAA AGCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Allosteric control of ?II-tryptase by a redox active disulfide bond.
The Journal of biological chemistry288(48)34920-9(2013 Nov)
Cook KM,McNeil HP,Hogg PJ

Identification and analysis of unitary pseudogenes: historic and contemporary gene losses in humans and other primates.
Genome biology11(3)R26(2010)
Zhang ZD,Frankish A,Hunt T,Harrow J,Gerstein M

Human subjects are protected from mast cell tryptase deficiency despite frequent inheritance of loss-of-function mutations.
The Journal of allergy and clinical immunology124(5)1099-105.e1-4(2009 Nov)
Trivedi NN,Tamraz B,Chu C,Kwok PY,Caughey GH

Systematic identification of SH3 domain-mediated human protein-protein interactions by peptide array target screening.
Proteomics7(11)1775-85(2007 Jun)
Wu C,Ma MH,Brown KR,Geisler M,Li L,Tzeng E,Jia CY,Jurisica I,Li SS

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TPSB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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