SPINK9 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPINK9
Entrez Gene ID	643394
Full Name	serine peptidase inhibitor, Kazal type 9
Synonyms	LEKTI2
General protein information	Preferred Names serine peptidase inhibitor, Kazal type 9 Names serine protease inhibitor Kazal-type 9 lymphoepithelial Kazal-type-related inhibitor 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q32
Summary	The protein encoded by this gene is a Kazal-type serine protease inhibitor that appears to specifically target kallikrein-related peptidase 5 (KLK5) in the palmo-plantar epidermis. KLK5 is an important initiator of skin desquamation, so the encoded protease inhibitor may regulate skin differentiation in the palms of hands and soles of feet. This cationic protein has also been shown to promote keratinocyte migration by activation of the epidermal growth factor receptor (EGFR). [provided by RefSeq, Dec 2015].
Disorder MIM:	613511

mRNA and Protein(s)

mRNA	Protein	Name
NM_001040433.1	NP_001035523.1	serine protease inhibitor Kazal-type 9 precursor
XM_017009709.1	XP_016865198.1	serine protease inhibitor Kazal-type 9 isoform X1
NM_001040433.2	NP_001035523.1	serine protease inhibitor Kazal-type 9 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPINK9	XP_005619511.1
Homo sapiens (human)	SPINK9	NP_001035523.1
Pan troglodytes (chimpanzee)	SPINK9	XP_001143757.1
	SPINK9	XP_001096727.1
Bos taurus (cattle)	SPINK9	XP_005209686.1

Gene Ontology
Bibliography

Related articles in PubMed

SPINK9 stimulates metalloprotease/EGFR-dependent keratinocyte migration via purinergic receptor activation.
Sperrhacke M, Fischer J, Wu Z, Kl?nder S, Sedlacek R, Schroeder JM, Meyer-Hoffert U, Reiss K
The Journal of investigative dermatology134(6)1645-1654(2014 Jun)

Characterization of SPINK9, a KLK5-specific inhibitor expressed in palmo-plantar epidermis.
Br?nnstr?m K, Ohman A, von Pawel Rammingen U, Olofsson A, Brattsand M
Biological chemistry393(5)369-77(2012 Apr)

Expression and regulation of murine SPINK12, a potential orthologue of human LEKTI2.
Reiss K, Meyer-Hoffert U, Fischer J, Sperrhacke M, Wu Z, Dimitrieva O, Krenek P, Suchanova S, Buryova H, Brauer R, Sedlacek R
Experimental dermatology20(11)905-10(2011 Nov)

SPINK9: a selective, skin-specific Kazal-type serine protease inhibitor.
Brattsand M, Stefansson K, Hubiche T, Nilsson SK, Egelrud T
The Journal of investigative dermatology129(7)1656-65(2009 Jul)

Identification of lympho-epithelial Kazal-type inhibitor 2 in human skin as a kallikrein-related peptidase 5-specific protease inhibitor.
Meyer-Hoffert U, Wu Z, Schr?der JM
PloS one4(2)e4372(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPINK9 has a role in stimulating metalloprotease/EGFR-dependent keratinocyte migration by purinergic receptor pathway activation
Title: SPINK9 stimulates metalloprotease/EGFR-dependent keratinocyte migration via purinergic receptor activation.

the inhibition and binding of different SPINK9 variants towards KLK5, KLK7, KLK8 and KLK14
Title: Characterization of SPINK9, a KLK5-specific inhibitor expressed in palmo-plantar epidermis.

mSPINK12 shows an inhibitory activity against murine keratinocyte-derived trypsin-like proteolytic activity, and thus, appears orthologous to human LEKTI2 and may play an role in the regulation of epithelial cell functions.
Title: Expression and regulation of murine SPINK12, a potential orthologue of human LEKTI2.

possible role for SPINK9 in the site-specific epidermal differentiation of palms and soles
Title: SPINK9: a selective, skin-specific Kazal-type serine protease inhibitor.

A new KLK5-inhibiting peptide encoded by a member of the Spink family, designated as Spink9 located on chromosome 5p33.1, was identified.
Title: Identification of lympho-epithelial Kazal-type inhibitor 2 in human skin as a kallikrein-related peptidase 5-specific protease inhibitor.

The following SPINK9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPINK9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25998	NM_001040433.2 Latest version!	Homo sapiens serine peptidase inhibitor Kazal type 9 (SPINK9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu95989	XM_017009709.1 Latest version!	Homo sapiens serine peptidase inhibitor Kazal type 9 (SPINK9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu25998	NM_001040433.1	Homo sapiens serine peptidase inhibitor, Kazal type 9 (SPINK9), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPINK9 ( NM_001040433.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035523.1 Homo sapiens serine peptidase inhibitor, Kazal type 9 (SPINK9), mRNA.

Price & Availability↑

CloneID	OHu25998
Clone ID Related Accession (Same CDS sequence)	NM_001040433.1 , NM_001040433.2
Accession Version	NM_001040433.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	serine protease inhibitor Kazal-type 9 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY396740.1. On or before May 5, 2006 this sequence version replaced XM_926728.1, XM_940086.1. ##Evidence-Data-START## Transcript exon combination :: AY396740.1, BC137530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465418 [ECO:0000348] ##Evidence-Data-END##

ATGAGAGCAA CAGCCATAGT CCTACTCTTG GCTCTGACAC TTGCAACCAT GTTCAGTATA 
GAATGTGCCA AACAGACGAA ACAGATGGTT GACTGCAGTC ATTATAAAAA GTTACCACCA 
GGACAACAGA GATTTTGTCA TCATATGTAT GATCCAATTT GTGGATCTGA TGGCAAAACT 
TATAAAAATG ATTGCTTCTT CTGTTCTAAA GTTAAGAAAA CTGACGGCAC ACTTAAATTT 
GTACATTTTG GAAAATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035523.1
CDS	56..316
Translation	MRATAIVLLLALTLATMFSIECAKQTKQMVDCSHYKKLPPGQQRFCHHMYDPICGSDGKTYKNDCFFCSKVKKTDGTLKFVHFGKC

Target ORF information:

RefSeq Version	NM_001040433.1
Organism	Homo sapiens(human)
Definition	Homo sapiens serine peptidase inhibitor, Kazal type 9 (SPINK9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040433.1

ORF Insert Sequence:

ATGAGAGCAA CAGCCATAGT CCTACTCTTG GCTCTGACAC TTGCAACCAT GTTCAGTATA 
GAATGTGCCA AACAGACGAA ACAGATGGTT GACTGCAGTC ATTATAAAAA GTTACCACCA 
GGACAACAGA GATTTTGTCA TCATATGTAT GATCCAATTT GTGGATCTGA TGGCAAAACT 
TATAAAAATG ATTGCTTCTT CTGTTCTAAA GTTAAGAAAA CTGACGGCAC ACTTAAATTT 
GTACATTTTG GAAAATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPINK9 ( NM_001040433.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001035523.1 Homo sapiens serine peptidase inhibitor Kazal type 9 (SPINK9), mRNA.

Price & Availability↑

CloneID	OHu25998
Clone ID Related Accession (Same CDS sequence)	NM_001040433.1 , NM_001040433.2
Accession Version	NM_001040433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	serine protease inhibitor Kazal-type 9 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY396740.1 and AC091948.3. On Nov 22, 2018 this sequence version replaced NM_001040433.1. ##Evidence-Data-START## Transcript exon combination :: AY396740.1, BC137530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465418 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000377906.2/ ENSP00000367139.1 RefSeq Select criteria :: based on computational evidence ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGAGCAA CAGCCATAGT CCTACTCTTG GCTCTGACAC TTGCAACCAT GTTCAGTATA 
GAATGTGCCA AACAGACGAA ACAGATGGTT GACTGCAGTC ATTATAAAAA GTTACCACCA 
GGACAACAGA GATTTTGTCA TCATATGTAT GATCCAATTT GTGGATCTGA TGGCAAAACT 
TATAAAAATG ATTGCTTCTT CTGTTCTAAA GTTAAGAAAA CTGACGGCAC ACTTAAATTT 
GTACATTTTG GAAAATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035523.1
CDS	56..316
Translation	MRATAIVLLLALTLATMFSIECAKQTKQMVDCSHYKKLPPGQQRFCHHMYDPICGSDGKTYKNDCFFCSKVKKTDGTLKFVHFGKC

Target ORF information:

RefSeq Version	NM_001040433.2
Organism	Homo sapiens(human)
Definition	Homo sapiens serine peptidase inhibitor Kazal type 9 (SPINK9), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040433.2

ORF Insert Sequence:

ATGAGAGCAA CAGCCATAGT CCTACTCTTG GCTCTGACAC TTGCAACCAT GTTCAGTATA 
GAATGTGCCA AACAGACGAA ACAGATGGTT GACTGCAGTC ATTATAAAAA GTTACCACCA 
GGACAACAGA GATTTTGTCA TCATATGTAT GATCCAATTT GTGGATCTGA TGGCAAAACT 
TATAAAAATG ATTGCTTCTT CTGTTCTAAA GTTAAGAAAA CTGACGGCAC ACTTAAATTT 
GTACATTTTG GAAAATGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPINK9 ( XM_017009709.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865198.1 Homo sapiens serine peptidase inhibitor Kazal type 9 (SPINK9), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu95989
Clone ID Related Accession (Same CDS sequence)	XM_017009709.1
Accession Version	XM_017009709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine protease inhibitor Kazal-type 9 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCTTTA TGAATCCTTC TCATCATTGT TTTCATTATA AAACTCCACC AAGCAGCTCA 
GGAGAACACA ATGAACCCAG GAGACTCATG GGCTTAACGC AACCTGAACA TTTACAAGGT 
ATAGAATGTG CCAAACAGAC GAAACAGATG GTTGACTGCA GTCATTATAA AAAGTTACCA 
CCAGGACAAC AGAGATTTTG TCATCATATG TATGATCCAA TTTGTGGATC TGATGGCAAA 
ACTTATAAAA ATGATTGCTT CTTCTGTTCT AAAGTTAAGA AAACTGACGG CACACTTAAA 
TTTGTACATT TTGGAAAATG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865198.1
CDS	533..856
Translation	MVFMNPSHHCFHYKTPPSSSGEHNEPRRLMGLTQPEHLQGIECAKQTKQMVDCSHYKKLPPGQQRFCHHMYDPICGSDGKTYKNDCFFCSKVKKTDGTLKFVHFGKC

Target ORF information:

RefSeq Version	XM_017009709.1
Organism	Homo sapiens(human)
Definition	Homo sapiens serine peptidase inhibitor Kazal type 9 (SPINK9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009709.1

ORF Insert Sequence:

ATGGTCTTTA TGAATCCTTC TCATCATTGT TTTCATTATA AAACTCCACC AAGCAGCTCA 
GGAGAACACA ATGAACCCAG GAGACTCATG GGCTTAACGC AACCTGAACA TTTACAAGGT 
ATAGAATGTG CCAAACAGAC GAAACAGATG GTTGACTGCA GTCATTATAA AAAGTTACCA 
CCAGGACAAC AGAGATTTTG TCATCATATG TATGATCCAA TTTGTGGATC TGATGGCAAA 
ACTTATAAAA ATGATTGCTT CTTCTGTTCT AAAGTTAAGA AAACTGACGG CACACTTAAA 
TTTGTACATT TTGGAAAATG TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPINK9 stimulates metalloprotease/EGFR-dependent keratinocyte migration via purinergic receptor activation.
The Journal of investigative dermatology134(6)1645-1654(2014 Jun)
Sperrhacke M,Fischer J,Wu Z,Kl?nder S,Sedlacek R,Schroeder JM,Meyer-Hoffert U,Reiss K

Characterization of SPINK9, a KLK5-specific inhibitor expressed in palmo-plantar epidermis.
Biological chemistry393(5)369-77(2012 Apr)
Br?nnstr?m K,Ohman A,von Pawel Rammingen U,Olofsson A,Brattsand M

Expression and regulation of murine SPINK12, a potential orthologue of human LEKTI2.
Experimental dermatology20(11)905-10(2011 Nov)
Reiss K,Meyer-Hoffert U,Fischer J,Sperrhacke M,Wu Z,Dimitrieva O,Krenek P,Suchanova S,Buryova H,Brauer R,Sedlacek R

SPINK9: a selective, skin-specific Kazal-type serine protease inhibitor.
The Journal of investigative dermatology129(7)1656-65(2009 Jul)
Brattsand M,Stefansson K,Hubiche T,Nilsson SK,Egelrud T

Identification of lympho-epithelial Kazal-type inhibitor 2 in human skin as a kallikrein-related peptidase 5-specific protease inhibitor.
PloS one4(2)e4372(2009)
Meyer-Hoffert U,Wu Z,Schr?der JM

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SPINK9 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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