SCT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCT
Entrez Gene ID	6343
Full Name	secretin
General protein information	Preferred Names secretin Names secretin prepro-secretin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.5
Summary	This gene encodes a member of the glucagon family of peptides. The encoded preproprotein is secreted by endocrine S cells in the proximal small intestinal mucosa as a prohormone, then proteolytically processed to generate the mature peptide hormone. The release of this active peptide hormone is stimulated by either fatty acids or acidic pH in the duodenum. This hormone stimulates the secretion of bile and bicarbonate in the duodenum, pancreatic and biliary ducts. [provided by RefSeq, Feb 2016].
Disorder MIM:	182099

mRNA and Protein(s)

mRNA	Protein	Name
XM_017018109.1	XP_016873598.1	secretin isoform X1
NM_021920.4	NP_068739.1	secretin preproprotein
NM_021920.3	NP_068739.1	secretin preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCT	NP_068739.1
Pan troglodytes (chimpanzee)	SCT	XP_001149616.2

Gene Ontology
Bibliography

Related articles in PubMed

The physiological roles of secretin and its receptor.
Afroze S, Meng F, Jensen K, McDaniel K, Rahal K, Onori P, Gaudio E, Alpini G, Glaser SS
Annals of translational medicine1(3)29(2013 Oct)

CD36-dependent signaling mediates fatty acid-induced gut release of secretin and cholecystokinin.
Sundaresan S, Shahid R, Riehl TE, Chandra R, Nassir F, Stenson WF, Liddle RA, Abumrad NA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(3)1191-202(2013 Mar)

A major lineage of enteroendocrine cells coexpress CCK, secretin, GIP, GLP-1, PYY, and neurotensin but not somatostatin.
Egerod KL, Engelstoft MS, Grunddal KV, N?hr MK, Secher A, Sakata I, Pedersen J, Windel?v JA, F?chtbauer EM, Olsen J, Sundler F, Christensen JP, Wierup N, Olsen JV, Holst JJ, Zigman JM, Poulsen SS, Schwartz TW
Endocrinology153(12)5782-95(2012 Dec)

Mapping spatial approximations between the amino terminus of secretin and each of the extracellular loops of its receptor using cysteine trapping.
Dong M, Xu X, Ball AM, Makhoul JA, Lam PC, Pinon DI, Orry A, Sexton PM, Abagyan R, Miller LJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(12)5092-105(2012 Dec)

Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1.
Lee LT, Tan-Un KC, Chow BK
Annals of the New York Academy of Sciences1070393-8(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CD36 is a major mediator of FA-induced release of CCK and secretin. These peptides contribute to the role of CD36 in fat absorption and to its pleiotropic metabolic effects.
Title: CD36-dependent signaling mediates fatty acid-induced gut release of secretin and cholecystokinin.

a lineage of mature enteroendocrine cells have the ability to coexpress members of a group of functionally related peptides: CCK, secretin, GIP, GLP-1, PYY, and neurotensin
Title: A major lineage of enteroendocrine cells coexpress CCK, secretin, GIP, GLP-1, PYY, and neurotensin but not somatostatin.

Data suggest that the interaction between secretin peptide residue H1 and secretin receptor residue W274 is most compatible with a hydrophobic interaction.
Title: Mapping spatial approximations between the amino terminus of secretin and each of the extracellular loops of its receptor using cysteine trapping.

binding and activity of a series of 11 truncated and lactam-constrained secretin(5-27) analogues at the prototypic member of this family, the secretin receptor
Title: Lactam constraints provide insights into the receptor-bound conformation of secretin and stabilize a receptor antagonist.

Secretin effect on increase in transcription of the tyrosine hydroxylase (Th) gene and modulate catecholamine secretion, was tested.
Title: Catecholamine biosynthesis and secretion: physiological and pharmacological effects of secretin.

The following SCT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09497	NM_021920.4 Latest version!	Homo sapiens secretin (SCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu92419	XM_017018109.1 Latest version!	Homo sapiens secretin (SCT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu09497	NM_021920.3	Homo sapiens secretin (SCT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCT ( NM_021920.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068739.1 Homo sapiens secretin (SCT), mRNA.

Price & Availability↑

CloneID	OHu09497
Clone ID Related Accession (Same CDS sequence)	NM_021920.4 , NM_021920.3
Accession Version	NM_021920.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	secretin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY019819.1, HY243621.1 and AP006284.2. On Nov 23, 2018 this sequence version replaced NM_021920.3. CCDS Note: No human mRNA or EST accessions have been deposited in public databases to support this CCDS, but the gene, transcript and protein have been well-characterized in the literature, e.g., PMIDs:2315322, 12160732, 15706223 and 16888198. Secretin proteins in other species also support this CCDS. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY019819.1, HY055530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000176195.4/ ENSP00000176195.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCCCC GGCCCCTCCT GCTGCTGCTG CTGCTCCTCG GGGGCTCCGC CGCGCGCCCC 
GCGCCCCCCA GGGCCCGGCG ACACTCAGAC GGGACGTTCA CCAGCGAGCT CAGCCGCCTG 
CGGGAGGGCG CGCGGCTCCA GCGGCTGCTA CAGGGCCTGG TGGGGAAGCG CAGCGAGCAG 
GACGCAGAGA ACAGCATGGC CTGGACCAGG CTCAGCGCGG GTCTGCTCTG CCCGTCAGGG 
TCCAACATGC CCATCCTGCA GGCCTGGATG CCCCTGGACG GGACCTGGTC TCCCTGGCTG 
CCCCCTGGGC CTATGGTTTC AGAACCAGCT GGCGCTGCTG CAGAAGGAAC CTTGCGGCCC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068739.1
CDS	39..404
Translation	MAPRPLLLLLLLLGGSAARPAPPRARRHSDGTFTSELSRLREGARLQRLLQGLVGKRSEQDAENSMAWTRLSAGLLCPSGSNMPILQAWMPLDGTWSPWLPPGPMVSEPAGAAAEGTLRPR

Target ORF information:

RefSeq Version	NM_021920.4
Organism	Homo sapiens(human)
Definition	Homo sapiens secretin (SCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021920.4

ORF Insert Sequence:

ATGGCCCCCC GGCCCCTCCT GCTGCTGCTG CTGCTCCTCG GGGGCTCCGC CGCGCGCCCC 
GCGCCCCCCA GGGCCCGGCG ACACTCAGAC GGGACGTTCA CCAGCGAGCT CAGCCGCCTG 
CGGGAGGGCG CGCGGCTCCA GCGGCTGCTA CAGGGCCTGG TGGGGAAGCG CAGCGAGCAG 
GACGCAGAGA ACAGCATGGC CTGGACCAGG CTCAGCGCGG GTCTGCTCTG CCCGTCAGGG 
TCCAACATGC CCATCCTGCA GGCCTGGATG CCCCTGGACG GGACCTGGTC TCCCTGGCTG 
CCCCCTGGGC CTATGGTTTC AGAACCAGCT GGCGCTGCTG CAGAAGGAAC CTTGCGGCCC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCT ( XM_017018109.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016873598.1 Homo sapiens secretin (SCT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu92419
Clone ID Related Accession (Same CDS sequence)	XM_017018109.1
Accession Version	XM_017018109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	secretin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACAC CGGCCCCCCA ACAGTCACGC CACGCTGAAG CTGGAGACGA ACTCGCCCCA 
GCAAGGGCCC GGCGACACTC AGACGGGACG TTCACCAGCG AGCTCAGCCG CCTGCGGGAG 
GGCGCGCGGC TCCAGCGGCT GCTACAGGGC CTGGTGGGGA AGCGCAGCGA GCAGGACGCA 
GAGAACAGCA TGGCCTGGAC CAGGCTCAGC GCGGGTCTGC TCTGCCCGTC AGGGTCCAAC 
ATGCCCATCC TGCAGGCCTG GATGCCCCTG GACGGGACCT GGTCTCCCTG GCTGCCCCCT 
GGGCCTATGG TTTCAGAACC AGCTGGCGCT GCTGCAGAAG GAACCTTGCG GCCCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016873598.1
CDS	100..459
Translation	MATPAPQQSRHAEAGDELAPARARRHSDGTFTSELSRLREGARLQRLLQGLVGKRSEQDAENSMAWTRLSAGLLCPSGSNMPILQAWMPLDGTWSPWLPPGPMVSEPAGAAAEGTLRPR

Target ORF information:

RefSeq Version	XM_017018109.1
Organism	Homo sapiens(human)
Definition	Homo sapiens secretin (SCT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017018109.1

ORF Insert Sequence:

ATGGCAACAC CGGCCCCCCA ACAGTCACGC CACGCTGAAG CTGGAGACGA ACTCGCCCCA 
GCAAGGGCCC GGCGACACTC AGACGGGACG TTCACCAGCG AGCTCAGCCG CCTGCGGGAG 
GGCGCGCGGC TCCAGCGGCT GCTACAGGGC CTGGTGGGGA AGCGCAGCGA GCAGGACGCA 
GAGAACAGCA TGGCCTGGAC CAGGCTCAGC GCGGGTCTGC TCTGCCCGTC AGGGTCCAAC 
ATGCCCATCC TGCAGGCCTG GATGCCCCTG GACGGGACCT GGTCTCCCTG GCTGCCCCCT 
GGGCCTATGG TTTCAGAACC AGCTGGCGCT GCTGCAGAAG GAACCTTGCG GCCCAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCT ( NM_021920.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068739.1 Homo sapiens secretin (SCT), mRNA.

Price & Availability↑

CloneID	OHu09497
Clone ID Related Accession (Same CDS sequence)	NM_021920.4 , NM_021920.3
Accession Version	NM_021920.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	secretin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY019819.1 and HY243621.1. On Feb 12, 2016 this sequence version replaced NM_021920.2. CCDS Note: No human mRNA or EST accessions have been deposited in public databases to support this CCDS, but the gene, transcript and protein have been well-characterized in the literature, e.g., PMIDs:2315322, 12160732, 15706223 and 16888198. Secretin proteins in other species also support this CCDS. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY019819.1, HY055530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCCCCC GGCCCCTCCT GCTGCTGCTG CTGCTCCTCG GGGGCTCCGC CGCGCGCCCC 
GCGCCCCCCA GGGCCCGGCG ACACTCAGAC GGGACGTTCA CCAGCGAGCT CAGCCGCCTG 
CGGGAGGGCG CGCGGCTCCA GCGGCTGCTA CAGGGCCTGG TGGGGAAGCG CAGCGAGCAG 
GACGCAGAGA ACAGCATGGC CTGGACCAGG CTCAGCGCGG GTCTGCTCTG CCCGTCAGGG 
TCCAACATGC CCATCCTGCA GGCCTGGATG CCCCTGGACG GGACCTGGTC TCCCTGGCTG 
CCCCCTGGGC CTATGGTTTC AGAACCAGCT GGCGCTGCTG CAGAAGGAAC CTTGCGGCCC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068739.1
CDS	98..463
Translation	MAPRPLLLLLLLLGGSAARPAPPRARRHSDGTFTSELSRLREGARLQRLLQGLVGKRSEQDAENSMAWTRLSAGLLCPSGSNMPILQAWMPLDGTWSPWLPPGPMVSEPAGAAAEGTLRPR

Target ORF information:

RefSeq Version	NM_021920.3
Organism	Homo sapiens(human)
Definition	Homo sapiens secretin (SCT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021920.3

ORF Insert Sequence:

ATGGCCCCCC GGCCCCTCCT GCTGCTGCTG CTGCTCCTCG GGGGCTCCGC CGCGCGCCCC 
GCGCCCCCCA GGGCCCGGCG ACACTCAGAC GGGACGTTCA CCAGCGAGCT CAGCCGCCTG 
CGGGAGGGCG CGCGGCTCCA GCGGCTGCTA CAGGGCCTGG TGGGGAAGCG CAGCGAGCAG 
GACGCAGAGA ACAGCATGGC CTGGACCAGG CTCAGCGCGG GTCTGCTCTG CCCGTCAGGG 
TCCAACATGC CCATCCTGCA GGCCTGGATG CCCCTGGACG GGACCTGGTC TCCCTGGCTG 
CCCCCTGGGC CTATGGTTTC AGAACCAGCT GGCGCTGCTG CAGAAGGAAC CTTGCGGCCC 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The physiological roles of secretin and its receptor.
Annals of translational medicine1(3)29(2013 Oct)
Afroze S,Meng F,Jensen K,McDaniel K,Rahal K,Onori P,Gaudio E,Alpini G,Glaser SS

CD36-dependent signaling mediates fatty acid-induced gut release of secretin and cholecystokinin.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology27(3)1191-202(2013 Mar)
Sundaresan S,Shahid R,Riehl TE,Chandra R,Nassir F,Stenson WF,Liddle RA,Abumrad NA

A major lineage of enteroendocrine cells coexpress CCK, secretin, GIP, GLP-1, PYY, and neurotensin but not somatostatin.
Endocrinology153(12)5782-95(2012 Dec)
Egerod KL,Engelstoft MS,Grunddal KV,N?hr MK,Secher A,Sakata I,Pedersen J,Windel?v JA,F?chtbauer EM,Olsen J,Sundler F,Christensen JP,Wierup N,Olsen JV,Holst JJ,Zigman JM,Poulsen SS,Schwartz TW

Mapping spatial approximations between the amino terminus of secretin and each of the extracellular loops of its receptor using cysteine trapping.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(12)5092-105(2012 Dec)
Dong M,Xu X,Ball AM,Makhoul JA,Lam PC,Pinon DI,Orry A,Sexton PM,Abagyan R,Miller LJ

Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1.
Annals of the New York Academy of Sciences1070393-8(2006 Jul)
Lee LT,Tan-Un KC,Chow BK

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

SCT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報