RNF5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF5
Entrez Gene ID	6048
Full Name	ring finger protein 5
Synonyms	RING5,RMA1
General protein information	Preferred Names ring finger protein 5 Names E3 ubiquitin-protein ligase RNF5 RING-type E3 ubiquitin transferase RNF5 protein G16 ram1 homolog ring finger protein 5, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	The protein encoded by this gene contains a RING finger, which is a motif known to be involved in protein-protein interactions. This protein is a membrane-bound ubiquitin ligase. It can regulate cell motility by targeting paxillin ubiquitination and altering the distribution and localization of paxillin in cytoplasm and cell focal adhesions. [provided by RefSeq, Jul 2008].
Disorder MIM:	602677

mRNA and Protein(s)

mRNA	Protein	Name
NM_006913.3	NP_008844.1	E3 ubiquitin-protein ligase RNF5
NM_006913.4	NP_008844.1	E3 ubiquitin-protein ligase RNF5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RNF5	XP_001164301.1
Canis lupus familiaris (dog)	RNF5	XP_532092.1
Homo sapiens (human)	RNF5	NP_008844.1
Xenopus tropicalis (tropical clawed frog)	rnf5	NP_001017071.1
Bos taurus (cattle)	RNF5	NP_001098915.1
Rattus norvegicus (Norway rat)	Rnf5	NP_001102495.1
	RNF5	XP_001114771.1
Mus musculus (house mouse)	Rnf5	NP_062276.1
Danio rerio (zebrafish)	LOC100537822	XP_003200552.1

Gene Ontology
Bibliography

Related articles in PubMed

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

Genetic Inhibition Of The Ubiquitin Ligase Rnf5 Attenuates Phenotypes Associated To F508del Cystic Fibrosis Mutation.
Tomati V, Sondo E, Armirotti A, Caci E, Pesce E, Marini M, Gianotti A, Jeon YJ, Cilli M, Pistorio A, Mastracci L, Ravazzolo R, Scholte B, Ronai Z, Galietta LJ, Pedemonte N
Scientific reports512138(2015 Jul)

Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies.
Jeon YJ, Khelifa S, Ratnikov B, Scott DA, Feng Y, Parisi F, Ruller C, Lau E, Kim H, Brill LM, Jiang T, Rimm DL, Cardiff RD, Mills GB, Smith JW, Osterman AL, Kluger Y, Ronai ZA
Cancer cell27(3)354-69(2015 Mar)

RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
El Khouri E, Le Pavec G, Toledano MB, Delaunay-Moisan A
The Journal of biological chemistry288(43)31177-91(2013 Oct)

Novel, gel-free proteomics approach identifies RNF5 and JAMP as modulators of GPCR stability.
Roy SJ, Glazkova I, Fr?chette L, Iorio-Morin C, Binda C, P?trin D, Trieu P, Robitaille M, Angers S, H?bert TE, Parent JL
Molecular endocrinology (Baltimore, Md.)27(8)1245-66(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we found that RNF5 is differentially expressed in human bronchial epithelia from CF vs. control patients. Our results identify RNF5 as a target for therapeutic modalities to antagonize mutant CFTR proteins.
Title: Genetic Inhibition Of The Ubiquitin Ligase Rnf5 Attenuates Phenotypes Associated To F508del Cystic Fibrosis Mutation.

RNF5 is involved in the regulation of the L-glutamine carrier proteins SLC1A5 and SLC38A2.
Title: Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies.

Our data suggest that RNF5 regulates the turnover of specific G protein-coupled receptors by ubiquitinating JAMP and preventing proteasome recruitment.
Title: Novel, gel-free proteomics approach identifies RNF5 and JAMP as modulators of GPCR stability.

In addition to confirming one of the top findings in the meta-analysis, TRIM26, RNF5 and HLA-DRB3 have been identified as potential candidate genes for schizophrenia.
Title: Expression QTL analysis of top loci from GWAS meta-analysis highlights additional schizophrenia candidate genes.

Rma1, an E3 ubiquitin ligase localized in the ER membrane, is involved in Pendrin degradation
Title: Roles of 17-AAG-induced molecular chaperones and Rma1 E3 ubiquitin ligase in folding and degradation of Pendrin.

The following RNF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14875	NM_006913.4 Latest version!	Homo sapiens ring finger protein 5 (RNF5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu14875	NM_006913.3	Homo sapiens ring finger protein 5 (RNF5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF5 ( NM_006913.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_008844.1 Homo sapiens ring finger protein 5 (RNF5), mRNA.

Price & Availability↑

CloneID	OHu14875
Clone ID Related Accession (Same CDS sequence)	NM_006913.3 , NM_006913.4
Accession Version	NM_006913.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM712189.1, BC127652.1 and BC004155.2. On Aug 31, 2007 this sequence version replaced NM_006913.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111392.1, ERR279870.1946.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG CGGAGGAGGA GGACGGGGGC CCCGAAGGGC CAAATCGCGA GCGGGGCGGG 
GCGGGCGCGA CCTTCGAATG TAATATATGT TTGGAGACTG CTCGGGAAGC TGTGGTCAGT 
GTGTGTGGCC ACCTGTACTG TTGGCCATGT CTTCATCAGT GGCTGGAGAC ACGGCCAGAA 
CGGCAAGAGT GTCCAGTATG TAAAGCTGGG ATCAGCAGAG AGAAGGTTGT CCCGCTTTAT 
GGGCGAGGGA GCCAGAAGCC CCAGGATCCC AGATTAAAAA CTCCACCCCG CCCCCAGGGC 
CAGAGACCAG CTCCGGAGAG CAGAGGGGGA TTCCAGCCAT TTGGTGATAC CGGGGGCTTC 
CACTTCTCAT TTGGTGTTGG TGCTTTTCCC TTTGGCTTTT TCACCACCGT CTTCAATGCC 
CATGAGCCTT TCCGCCGGGG TACAGGTGTG GATCTGGGAC AGGGTCACCC AGCCTCCAGC 
TGGCAGGATT CCCTCTTCCT GTTTCTCGCC ATCTTCTTCT TTTTTTGGCT GCTCAGTATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008844.1
CDS	128..670
Translation	MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFNAHEPFRRGTGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI

Target ORF information:

RefSeq Version	NM_006913.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 5 (RNF5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006913.3

ORF Insert Sequence:

ATGGCAGCAG CGGAGGAGGA GGACGGGGGC CCCGAAGGGC CAAATCGCGA GCGGGGCGGG 
GCGGGCGCGA CCTTCGAATG TAATATATGT TTGGAGACTG CTCGGGAAGC TGTGGTCAGT 
GTGTGTGGCC ACCTGTACTG TTGGCCATGT CTTCATCAGT GGCTGGAGAC ACGGCCAGAA 
CGGCAAGAGT GTCCAGTATG TAAAGCTGGG ATCAGCAGAG AGAAGGTTGT CCCGCTTTAT 
GGGCGAGGGA GCCAGAAGCC CCAGGATCCC AGATTAAAAA CTCCACCCCG CCCCCAGGGC 
CAGAGACCAG CTCCGGAGAG CAGAGGGGGA TTCCAGCCAT TTGGTGATAC CGGGGGCTTC 
CACTTCTCAT TTGGTGTTGG TGCTTTTCCC TTTGGCTTTT TCACCACCGT CTTCAATGCC 
CATGAGCCTT TCCGCCGGGG TACAGGTGTG GATCTGGGAC AGGGTCACCC AGCCTCCAGC 
TGGCAGGATT CCCTCTTCCT GTTTCTCGCC ATCTTCTTCT TTTTTTGGCT GCTCAGTATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF5 ( NM_006913.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_008844.1 Homo sapiens ring finger protein 5 (RNF5), mRNA.

Price & Availability↑

CloneID	OHu14875
Clone ID Related Accession (Same CDS sequence)	NM_006913.3 , NM_006913.4
Accession Version	NM_006913.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RNF5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM712189.1, BC127652.1 and BC004155.2. On Nov 23, 2018 this sequence version replaced NM_006913.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111392.1, SRR5189667.389884.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000375094.4/ ENSP00000364235.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCAG CGGAGGAGGA GGACGGGGGC CCCGAAGGGC CAAATCGCGA GCGGGGCGGG 
GCGGGCGCGA CCTTCGAATG TAATATATGT TTGGAGACTG CTCGGGAAGC TGTGGTCAGT 
GTGTGTGGCC ACCTGTACTG TTGGCCATGT CTTCATCAGT GGCTGGAGAC ACGGCCAGAA 
CGGCAAGAGT GTCCAGTATG TAAAGCTGGG ATCAGCAGAG AGAAGGTTGT CCCGCTTTAT 
GGGCGAGGGA GCCAGAAGCC CCAGGATCCC AGATTAAAAA CTCCACCCCG CCCCCAGGGC 
CAGAGACCAG CTCCGGAGAG CAGAGGGGGA TTCCAGCCAT TTGGTGATAC CGGGGGCTTC 
CACTTCTCAT TTGGTGTTGG TGCTTTTCCC TTTGGCTTTT TCACCACCGT CTTCAATGCC 
CATGAGCCTT TCCGCCGGGG TACAGGTGTG GATCTGGGAC AGGGTCACCC AGCCTCCAGC 
TGGCAGGATT CCCTCTTCCT GTTTCTCGCC ATCTTCTTCT TTTTTTGGCT GCTCAGTATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008844.1
CDS	108..650
Translation	MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFGVGAFPFGFFTTVFNAHEPFRRGTGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI

Target ORF information:

RefSeq Version	NM_006913.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 5 (RNF5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006913.4

ORF Insert Sequence:

ATGGCAGCAG CGGAGGAGGA GGACGGGGGC CCCGAAGGGC CAAATCGCGA GCGGGGCGGG 
GCGGGCGCGA CCTTCGAATG TAATATATGT TTGGAGACTG CTCGGGAAGC TGTGGTCAGT 
GTGTGTGGCC ACCTGTACTG TTGGCCATGT CTTCATCAGT GGCTGGAGAC ACGGCCAGAA 
CGGCAAGAGT GTCCAGTATG TAAAGCTGGG ATCAGCAGAG AGAAGGTTGT CCCGCTTTAT 
GGGCGAGGGA GCCAGAAGCC CCAGGATCCC AGATTAAAAA CTCCACCCCG CCCCCAGGGC 
CAGAGACCAG CTCCGGAGAG CAGAGGGGGA TTCCAGCCAT TTGGTGATAC CGGGGGCTTC 
CACTTCTCAT TTGGTGTTGG TGCTTTTCCC TTTGGCTTTT TCACCACCGT CTTCAATGCC 
CATGAGCCTT TCCGCCGGGG TACAGGTGTG GATCTGGGAC AGGGTCACCC AGCCTCCAGC 
TGGCAGGATT CCCTCTTCCT GTTTCTCGCC ATCTTCTTCT TTTTTTGGCT GCTCAGTATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

Genetic Inhibition Of The Ubiquitin Ligase Rnf5 Attenuates Phenotypes Associated To F508del Cystic Fibrosis Mutation.
Scientific reports512138(2015 Jul)
Tomati V,Sondo E,Armirotti A,Caci E,Pesce E,Marini M,Gianotti A,Jeon YJ,Cilli M,Pistorio A,Mastracci L,Ravazzolo R,Scholte B,Ronai Z,Galietta LJ,Pedemonte N

Regulation of glutamine carrier proteins by RNF5 determines breast cancer response to ER stress-inducing chemotherapies.
Cancer cell27(3)354-69(2015 Mar)
Jeon YJ,Khelifa S,Ratnikov B,Scott DA,Feng Y,Parisi F,Ruller C,Lau E,Kim H,Brill LM,Jiang T,Rimm DL,Cardiff RD,Mills GB,Smith JW,Osterman AL,Kluger Y,Ronai ZA

RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
The Journal of biological chemistry288(43)31177-91(2013 Oct)
El Khouri E,Le Pavec G,Toledano MB,Delaunay-Moisan A

Novel, gel-free proteomics approach identifies RNF5 and JAMP as modulators of GPCR stability.
Molecular endocrinology (Baltimore, Md.)27(8)1245-66(2013 Aug)
Roy SJ,Glazkova I,Fr?chette L,Iorio-Morin C,Binda C,P?trin D,Trieu P,Robitaille M,Angers S,H?bert TE,Parent JL

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RNF5 cDNA ORF clone, Homo sapiens(human)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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