Rnf5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rnf5
Entrez Gene ID	407784
Full Name	ring finger protein 5
General protein information	Preferred Names ring finger protein 5 Names E3 ubiquitin-protein ligase RNF5 RING-type E3 ubiquitin transferase RNF5 ing finger protein 5 pseudogene ring finger protein 5, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001109025.2	NP_001102495.1	E3 ubiquitin-protein ligase RNF5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RNF5	XP_001164301.1
Canis lupus familiaris (dog)	RNF5	XP_532092.1
Homo sapiens (human)	RNF5	NP_008844.1
Xenopus tropicalis (tropical clawed frog)	rnf5	NP_001017071.1
Bos taurus (cattle)	RNF5	NP_001098915.1
Rattus norvegicus (Norway rat)	Rnf5	NP_001102495.1
	RNF5	XP_001114771.1
Mus musculus (house mouse)	Rnf5	NP_062276.1
Danio rerio (zebrafish)	LOC100537822	XP_003200552.1

Gene Ontology
Bibliography

Related articles in PubMed

RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
El Khouri E, Le Pavec G, Toledano MB, Delaunay-Moisan A
The Journal of biological chemistry288(43)31177-91(2013 Oct)

Regulation of ATG4B stability by RNF5 limits basal levels of autophagy and influences susceptibility to bacterial infection.
Kuang E, Okumura CY, Sheffy-Levin S, Varsano T, Shu VC, Qi J, Niesman IR, Yang HJ, L?pez-Ot?n C, Yang WY, Reed JC, Broday L, Nizet V, Ronai ZA
PLoS genetics8(10)e1003007(2012)

Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP).
Tcherpakov M, Delaunay A, Toth J, Kadoya T, Petroski MD, Ronai ZA
The Journal of biological chemistry284(18)12099-109(2009 May)

The ubiquitin ligase RNF5 regulates antiviral responses by mediating degradation of the adaptor protein MITA.
Zhong B, Zhang L, Lei C, Li Y, Mao AP, Yang Y, Wang YY, Zhang XL, Shu HB
Immunity30(3)397-407(2009 Mar)

The genomic sequence and comparative analysis of the rat major histocompatibility complex.
Hurt P, Walter L, Sudbrak R, Klages S, M?ller I, Shiina T, Inoko H, Lehrach H, G?nther E, Reinhardt R, Himmelbauer H
Genome research14(4)631-9(2004 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rnf5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rnf5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00626	NM_001109025.2 Latest version!	Rattus norvegicus ring finger protein 5 (Rnf5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rnf5 ( NM_001109025.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102495.1 Rattus norvegicus ring finger protein 5 (Rnf5), mRNA.

Price & Availability↑

CloneID	ORa00626
Clone ID Related Accession (Same CDS sequence)	NM_001109025.2
Accession Version	NM_001109025.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase RNF5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DY573537.1 and BC088341.1. On Dec 27, 2007 this sequence version replaced NM_001109025.1. ##Evidence-Data-START## Transcript exon combination :: BC088341.1, DV721801.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCAGCAG CAGAGGAAGA GGACGGGGGC CCCGAAGGGC CAAATCGCGA GCGGGGCGGG 
GCGAGCGCGA CCTTCGAATG TAATATATGT TTGGAGACAG CTCGCGAAGC TGTGGTCAGC 
GTGTGTGGCC ACCTCTATTG TTGGCCCTGT CTCCATCAGT GGCTGGAGAC ACGGCCAGAC 
CGGCAAGAAT GCCCGGTGTG TAAAGCCGGA ATAAGCAGGG AGAAGGTCGT CCCTCTTTAT 
GGTCGAGGGA GCCAGAAGCC ACAGGATCCC AGATTGAAAA CCCCACCCCG CCCTCAGGGC 
CAGCGGCCAG CTCCGGAGAG CAGAGGGGGG TTCCAGCCAT TCGGTGATGC AGGGGGATTT 
CACTTCTCCT TTGGTGTTGG CGCCTTCCCC TTTGGCTTCT TTACCACCGT GTTCAATGCC 
CACGAGCCTT TCCGAAGGGG TGCAGGTGTG GATCTGGGGC AGGGTCACCC AGCCTCCAGC 
TGGCAAGATT CCTTGTTCCT GTTCCTCGCC ATCTTTTTCT TTTTCTGGCT GCTCAGTATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102495.1
CDS	99..641
Translation	MAAAEEEDGGPEGPNRERGGASATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPDRQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDAGGFHFSFGVGAFPFGFFTTVFNAHEPFRRGAGVDLGQGHPASSWQDSLFLFLAIFFFFWLLSI

Target ORF information:

RefSeq Version	NM_001109025.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ring finger protein 5 (Rnf5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109025.2

ORF Insert Sequence:

ATGGCAGCAG CAGAGGAAGA GGACGGGGGC CCCGAAGGGC CAAATCGCGA GCGGGGCGGG 
GCGAGCGCGA CCTTCGAATG TAATATATGT TTGGAGACAG CTCGCGAAGC TGTGGTCAGC 
GTGTGTGGCC ACCTCTATTG TTGGCCCTGT CTCCATCAGT GGCTGGAGAC ACGGCCAGAC 
CGGCAAGAAT GCCCGGTGTG TAAAGCCGGA ATAAGCAGGG AGAAGGTCGT CCCTCTTTAT 
GGTCGAGGGA GCCAGAAGCC ACAGGATCCC AGATTGAAAA CCCCACCCCG CCCTCAGGGC 
CAGCGGCCAG CTCCGGAGAG CAGAGGGGGG TTCCAGCCAT TCGGTGATGC AGGGGGATTT 
CACTTCTCCT TTGGTGTTGG CGCCTTCCCC TTTGGCTTCT TTACCACCGT GTTCAATGCC 
CACGAGCCTT TCCGAAGGGG TGCAGGTGTG GATCTGGGGC AGGGTCACCC AGCCTCCAGC 
TGGCAAGATT CCTTGTTCCT GTTCCTCGCC ATCTTTTTCT TTTTCTGGCT GCTCAGTATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RNF185 is a novel E3 ligase of endoplasmic reticulum-associated degradation (ERAD) that targets cystic fibrosis transmembrane conductance regulator (CFTR).
The Journal of biological chemistry288(43)31177-91(2013 Oct)
El Khouri E,Le Pavec G,Toledano MB,Delaunay-Moisan A

Regulation of ATG4B stability by RNF5 limits basal levels of autophagy and influences susceptibility to bacterial infection.
PLoS genetics8(10)e1003007(2012)
Kuang E,Okumura CY,Sheffy-Levin S,Varsano T,Shu VC,Qi J,Niesman IR,Yang HJ,L?pez-Ot?n C,Yang WY,Reed JC,Broday L,Nizet V,Ronai ZA

Regulation of endoplasmic reticulum-associated degradation by RNF5-dependent ubiquitination of JNK-associated membrane protein (JAMP).
The Journal of biological chemistry284(18)12099-109(2009 May)
Tcherpakov M,Delaunay A,Toth J,Kadoya T,Petroski MD,Ronai ZA

The ubiquitin ligase RNF5 regulates antiviral responses by mediating degradation of the adaptor protein MITA.
Immunity30(3)397-407(2009 Mar)
Zhong B,Zhang L,Lei C,Li Y,Mao AP,Yang Y,Wang YY,Zhang XL,Shu HB

The genomic sequence and comparative analysis of the rat major histocompatibility complex.
Genome research14(4)631-9(2004 Apr)
Hurt P,Walter L,Sudbrak R,Klages S,M?ller I,Shiina T,Inoko H,Lehrach H,G?nther E,Reinhardt R,Himmelbauer H

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Rnf5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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