RING1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RING1
Entrez Gene ID	6015
Full Name	ring finger protein 1
Synonyms	RING1A,RNF1
General protein information	Preferred Names ring finger protein 1 Names E3 ubiquitin-protein ligase RING1 RING-type E3 ubiquitin transferase RING1 polycomb complex protein RING1 really interesting new gene 1 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.32
Summary	This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc-binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine-rich C-terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4. [provided by RefSeq, Jul 2008].
Disorder MIM:	602045

mRNA and Protein(s)

mRNA	Protein	Name
NM_002931.4	NP_002922.2	E3 ubiquitin-protein ligase RING1
NM_002931.3	NP_002922.2	E3 ubiquitin-protein ligase RING1

Pathways from BioSystems

Homologies

	RING1	NP_001108431.1
Bos taurus (cattle)	RING1	NP_001098521.1
Homo sapiens (human)	RING1	NP_002922.2
Canis lupus familiaris (dog)	RING1	NP_001041593.1
Mus musculus (house mouse)	Ring1	NP_033092.3
Rattus norvegicus (Norway rat)	Ring1	NP_997714.2
Xenopus tropicalis (tropical clawed frog)	ring1	NP_001090794.1
Pan troglodytes (chimpanzee)	RING1	NP_001074951.1

Gene Ontology
Bibliography

Related articles in PubMed

The E3 Ligase RING1 Targets p53 for Degradation and Promotes Cancer Cell Proliferation and Survival.
Shen J, Li P, Shao X, Yang Y, Liu X, Feng M, Yu Q, Hu R, Wang Z
Cancer research78(2)359-371(2018 Jan)

Nuclear envelope-distributed CD147 interacts with and inhibits the transcriptional function of RING1 and promotes melanoma cell motility.
Chen J, Peng C, Lei L, Zhang J, Zeng W, Chen X
PloS one12(8)e0183689(2017)

A High-Density Map for Navigating the Human Polycomb Complexome.
Hauri S, Comoglio F, Seimiya M, Gerstung M, Glatter T, Hansen K, Aebersold R, Paro R, Gstaiger M, Beisel C
Cell reports17(2)583-595(2016 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study identified novel interactions between CD147 and RING1, recovered CD147 nuclear envelope distribution in melanoma cells, and suggested a new mechanism underlying how cytoplasmic CD147 promotes melanoma development
Title: Nuclear envelope-distributed CD147 interacts with and inhibits the transcriptional function of RING1 and promotes melanoma cell motility.

The release of epigenetic repression by nitrosylation of RING1A is critical for effective cell transdifferentiation.
Title: Transdifferentiation Requires iNOS Activation: Role of RING1A S-Nitrosylation.

The polycomb complex proteins BMI1 and RING1A regulate the ubiquitination of chromatin associated proteins bound to promoters.
Title: RING1A and BMI1 bookmark active genes via ubiquitination of chromatin-associated proteins.

On the basis of these results, we proposed that the expression of Ring1 protein may be a novel indicator of hepatocellular carcinoma prognosis.
Title: Upregulated expression of polycomb protein Ring1 contributes to poor prognosis and accelerated proliferation in human hepatocellular carcinoma.

HSCARG interacts with RING1 and inhibits RING1 ubiquitination.
Title: HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation.

The following RING1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RING1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30158	NM_002931.4 Latest version!	Homo sapiens ring finger protein 1 (RING1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu30158	NM_002931.3	Homo sapiens ring finger protein 1 (RING1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RING1 ( NM_002931.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002922.2 Homo sapiens ring finger protein 1 (RING1), mRNA.

Price & Availability↑

CloneID	OHu30158
Clone ID Related Accession (Same CDS sequence)	NM_002931.4 , NM_002931.3
Accession Version	NM_002931.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RING1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL645940.4, BI259576.1 and BC051866.2. On Nov 23, 2018 this sequence version replaced NM_002931.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.6253.1, SRR3476690.734216.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374656.5/ ENSP00000363787.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGACGC CGGCGAATGC CCAGAATGCC AGCAAAACGT GGGAACTGAG TCTGTATGAG 
CTGCACCGGA CCCCGCAGGA AGCCATAATG GATGGCACAG AGATTGCTGT TTCCCCTCGG 
TCACTGCATT CAGAACTCAT GTGCCCTATC TGCCTGGACA TGCTGAAGAA TACGATGACC 
ACCAAGGAGT GCCTCCACAG ATTCTGCTCT GACTGCATTG TCACAGCCCT ACGGAGCGGG 
AACAAGGAGT GTCCTACCTG CCGAAAGAAG CTGGTGTCCA AGCGATCCCT ACGGCCAGAC 
CCCAACTTTG ATGCCCTGAT CTCTAAGATC TATCCTAGCC GGGAGGAATA CGAGGCCCAT 
CAAGACCGAG TGCTTATCCG CCTGAGCCGC CTGCACAACC AGCAGGCATT GAGCTCCAGC 
ATTGAGGAGG GGCTACGCAT GCAGGCCATG CACAGGGCCC AGCGTGTGAG GCGGCCGATA 
CCAGGGTCAG ATCAGACCAC AACGATGAGT GGGGGGGAAG GAGAGCCCGG GGAGGGAGAA 
GGGGATGGAG AAGATGTGAG CTCAGACTCC GCCCCTGACT CTGCCCCAGG CCCTGCTCCC 
AAGCGACCCC GTGGAGGGGG CGCAGGGGGG AGCAGTGTAG GGACAGGGGG AGGCGGCACT 
GGTGGGGTGG GTGGGGGTGC CGGTTCGGAA GACTCTGGTG ACCGGGGAGG GACTCTGGGA 
GGGGGAACGC TGGGCCCCCC AAGCCCTCCT GGGGCCCCCA GCCCCCCAGA GCCAGGTGGA 
GAAATTGAGC TCGTGTTCCG GCCCCACCCC CTGCTCGTGG AGAAGGGAGA ATACTGCCAG 
ACGAGGTATG TGAAGACAAC TGGGAATGCC ACAGTGGACC ACCTCTCCAA GTACTTGGCC 
CTGCGCATTG CCCTCGAGCG GAGGCAACAG CAGGAAGCAG GGGAGCCAGG AGGGCCTGGA 
GGGGGCGCCT CTGACACCGG AGGACCTGAT GGGTGTGGCG GGGAGGGTGG GGGTGCCGGA 
GGAGGTGACG GTCCTGAGGA GCCTGCTTTG CCCAGCCTGG AGGGCGTCAG TGAAAAGCAG 
TACACCATCT ACATCGCACC TGGAGGCGGG GCGTTCACGA CGTTGAATGG CTCGCTGACC 
CTGGAGCTGG TGAATGAGAA ATTCTGGAAG GTGTCCCGGC CACTGGAGCT GTGCTATGCT 
CCCACCAAGG ATCCAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002922.2
CDS	204..1424
Translation	MTTPANAQNASKTWELSLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVGTGGGGTGGVGGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYCQTRYVKTTGNATVDHLSKYLALRIALERRQQQEAGEPGGPGGGASDTGGPDGCGGEGGGAGGGDGPEEPALPSLEGVSEKQYTIYIAPGGGAFTTLNGSLTLELVNEKFWKVSRPLELCYAPTKDPK

Target ORF information:

RefSeq Version	NM_002931.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 1 (RING1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002931.4

ORF Insert Sequence:

ATGACGACGC CGGCGAATGC CCAGAATGCC AGCAAAACGT GGGAACTGAG TCTGTATGAG 
CTGCACCGGA CCCCGCAGGA AGCCATAATG GATGGCACAG AGATTGCTGT TTCCCCTCGG 
TCACTGCATT CAGAACTCAT GTGCCCTATC TGCCTGGACA TGCTGAAGAA TACGATGACC 
ACCAAGGAGT GCCTCCACAG ATTCTGCTCT GACTGCATTG TCACAGCCCT ACGGAGCGGG 
AACAAGGAGT GTCCTACCTG CCGAAAGAAG CTGGTGTCCA AGCGATCCCT ACGGCCAGAC 
CCCAACTTTG ATGCCCTGAT CTCTAAGATC TATCCTAGCC GGGAGGAATA CGAGGCCCAT 
CAAGACCGAG TGCTTATCCG CCTGAGCCGC CTGCACAACC AGCAGGCATT GAGCTCCAGC 
ATTGAGGAGG GGCTACGCAT GCAGGCCATG CACAGGGCCC AGCGTGTGAG GCGGCCGATA 
CCAGGGTCAG ATCAGACCAC AACGATGAGT GGGGGGGAAG GAGAGCCCGG GGAGGGAGAA 
GGGGATGGAG AAGATGTGAG CTCAGACTCC GCCCCTGACT CTGCCCCAGG CCCTGCTCCC 
AAGCGACCCC GTGGAGGGGG CGCAGGGGGG AGCAGTGTAG GGACAGGGGG AGGCGGCACT 
GGTGGGGTGG GTGGGGGTGC CGGTTCGGAA GACTCTGGTG ACCGGGGAGG GACTCTGGGA 
GGGGGAACGC TGGGCCCCCC AAGCCCTCCT GGGGCCCCCA GCCCCCCAGA GCCAGGTGGA 
GAAATTGAGC TCGTGTTCCG GCCCCACCCC CTGCTCGTGG AGAAGGGAGA ATACTGCCAG 
ACGAGGTATG TGAAGACAAC TGGGAATGCC ACAGTGGACC ACCTCTCCAA GTACTTGGCC 
CTGCGCATTG CCCTCGAGCG GAGGCAACAG CAGGAAGCAG GGGAGCCAGG AGGGCCTGGA 
GGGGGCGCCT CTGACACCGG AGGACCTGAT GGGTGTGGCG GGGAGGGTGG GGGTGCCGGA 
GGAGGTGACG GTCCTGAGGA GCCTGCTTTG CCCAGCCTGG AGGGCGTCAG TGAAAAGCAG 
TACACCATCT ACATCGCACC TGGAGGCGGG GCGTTCACGA CGTTGAATGG CTCGCTGACC 
CTGGAGCTGG TGAATGAGAA ATTCTGGAAG GTGTCCCGGC CACTGGAGCT GTGCTATGCT 
CCCACCAAGG ATCCAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RING1 ( NM_002931.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002922.2 Homo sapiens ring finger protein 1 (RING1), mRNA.

Price & Availability↑

CloneID	OHu30158
Clone ID Related Accession (Same CDS sequence)	NM_002931.4 , NM_002931.3
Accession Version	NM_002931.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase RING1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI259576.1, BC051866.2 and AI828228.1. On Aug 20, 2004 this sequence version replaced NM_002931.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.269681.1, AK289355.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGACGC CGGCGAATGC CCAGAATGCC AGCAAAACGT GGGAACTGAG TCTGTATGAG 
CTGCACCGGA CCCCGCAGGA AGCCATAATG GATGGCACAG AGATTGCTGT TTCCCCTCGG 
TCACTGCATT CAGAACTCAT GTGCCCTATC TGCCTGGACA TGCTGAAGAA TACGATGACC 
ACCAAGGAGT GCCTCCACAG ATTCTGCTCT GACTGCATTG TCACAGCCCT ACGGAGCGGG 
AACAAGGAGT GTCCTACCTG CCGAAAGAAG CTGGTGTCCA AGCGATCCCT ACGGCCAGAC 
CCCAACTTTG ATGCCCTGAT CTCTAAGATC TATCCTAGCC GGGAGGAATA CGAGGCCCAT 
CAAGACCGAG TGCTTATCCG CCTGAGCCGC CTGCACAACC AGCAGGCATT GAGCTCCAGC 
ATTGAGGAGG GGCTACGCAT GCAGGCCATG CACAGGGCCC AGCGTGTGAG GCGGCCGATA 
CCAGGGTCAG ATCAGACCAC AACGATGAGT GGGGGGGAAG GAGAGCCCGG GGAGGGAGAA 
GGGGATGGAG AAGATGTGAG CTCAGACTCC GCCCCTGACT CTGCCCCAGG CCCTGCTCCC 
AAGCGACCCC GTGGAGGGGG CGCAGGGGGG AGCAGTGTAG GGACAGGGGG AGGCGGCACT 
GGTGGGGTGG GTGGGGGTGC CGGTTCGGAA GACTCTGGTG ACCGGGGAGG GACTCTGGGA 
GGGGGAACGC TGGGCCCCCC AAGCCCTCCT GGGGCCCCCA GCCCCCCAGA GCCAGGTGGA 
GAAATTGAGC TCGTGTTCCG GCCCCACCCC CTGCTCGTGG AGAAGGGAGA ATACTGCCAG 
ACGAGGTATG TGAAGACAAC TGGGAATGCC ACAGTGGACC ACCTCTCCAA GTACTTGGCC 
CTGCGCATTG CCCTCGAGCG GAGGCAACAG CAGGAAGCAG GGGAGCCAGG AGGGCCTGGA 
GGGGGCGCCT CTGACACCGG AGGACCTGAT GGGTGTGGCG GGGAGGGTGG GGGTGCCGGA 
GGAGGTGACG GTCCTGAGGA GCCTGCTTTG CCCAGCCTGG AGGGCGTCAG TGAAAAGCAG 
TACACCATCT ACATCGCACC TGGAGGCGGG GCGTTCACGA CGTTGAATGG CTCGCTGACC 
CTGGAGCTGG TGAATGAGAA ATTCTGGAAG GTGTCCCGGC CACTGGAGCT GTGCTATGCT 
CCCACCAAGG ATCCAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002922.2
CDS	195..1415
Translation	MTTPANAQNASKTWELSLYELHRTPQEAIMDGTEIAVSPRSLHSELMCPICLDMLKNTMTTKECLHRFCSDCIVTALRSGNKECPTCRKKLVSKRSLRPDPNFDALISKIYPSREEYEAHQDRVLIRLSRLHNQQALSSSIEEGLRMQAMHRAQRVRRPIPGSDQTTTMSGGEGEPGEGEGDGEDVSSDSAPDSAPGPAPKRPRGGGAGGSSVGTGGGGTGGVGGGAGSEDSGDRGGTLGGGTLGPPSPPGAPSPPEPGGEIELVFRPHPLLVEKGEYCQTRYVKTTGNATVDHLSKYLALRIALERRQQQEAGEPGGPGGGASDTGGPDGCGGEGGGAGGGDGPEEPALPSLEGVSEKQYTIYIAPGGGAFTTLNGSLTLELVNEKFWKVSRPLELCYAPTKDPK

Target ORF information:

RefSeq Version	NM_002931.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger protein 1 (RING1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002931.3

ORF Insert Sequence:

ATGACGACGC CGGCGAATGC CCAGAATGCC AGCAAAACGT GGGAACTGAG TCTGTATGAG 
CTGCACCGGA CCCCGCAGGA AGCCATAATG GATGGCACAG AGATTGCTGT TTCCCCTCGG 
TCACTGCATT CAGAACTCAT GTGCCCTATC TGCCTGGACA TGCTGAAGAA TACGATGACC 
ACCAAGGAGT GCCTCCACAG ATTCTGCTCT GACTGCATTG TCACAGCCCT ACGGAGCGGG 
AACAAGGAGT GTCCTACCTG CCGAAAGAAG CTGGTGTCCA AGCGATCCCT ACGGCCAGAC 
CCCAACTTTG ATGCCCTGAT CTCTAAGATC TATCCTAGCC GGGAGGAATA CGAGGCCCAT 
CAAGACCGAG TGCTTATCCG CCTGAGCCGC CTGCACAACC AGCAGGCATT GAGCTCCAGC 
ATTGAGGAGG GGCTACGCAT GCAGGCCATG CACAGGGCCC AGCGTGTGAG GCGGCCGATA 
CCAGGGTCAG ATCAGACCAC AACGATGAGT GGGGGGGAAG GAGAGCCCGG GGAGGGAGAA 
GGGGATGGAG AAGATGTGAG CTCAGACTCC GCCCCTGACT CTGCCCCAGG CCCTGCTCCC 
AAGCGACCCC GTGGAGGGGG CGCAGGGGGG AGCAGTGTAG GGACAGGGGG AGGCGGCACT 
GGTGGGGTGG GTGGGGGTGC CGGTTCGGAA GACTCTGGTG ACCGGGGAGG GACTCTGGGA 
GGGGGAACGC TGGGCCCCCC AAGCCCTCCT GGGGCCCCCA GCCCCCCAGA GCCAGGTGGA 
GAAATTGAGC TCGTGTTCCG GCCCCACCCC CTGCTCGTGG AGAAGGGAGA ATACTGCCAG 
ACGAGGTATG TGAAGACAAC TGGGAATGCC ACAGTGGACC ACCTCTCCAA GTACTTGGCC 
CTGCGCATTG CCCTCGAGCG GAGGCAACAG CAGGAAGCAG GGGAGCCAGG AGGGCCTGGA 
GGGGGCGCCT CTGACACCGG AGGACCTGAT GGGTGTGGCG GGGAGGGTGG GGGTGCCGGA 
GGAGGTGACG GTCCTGAGGA GCCTGCTTTG CCCAGCCTGG AGGGCGTCAG TGAAAAGCAG 
TACACCATCT ACATCGCACC TGGAGGCGGG GCGTTCACGA CGTTGAATGG CTCGCTGACC 
CTGGAGCTGG TGAATGAGAA ATTCTGGAAG GTGTCCCGGC CACTGGAGCT GTGCTATGCT 
CCCACCAAGG ATCCAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The E3 Ligase RING1 Targets p53 for Degradation and Promotes Cancer Cell Proliferation and Survival.
Cancer research78(2)359-371(2018 Jan)
Shen J,Li P,Shao X,Yang Y,Liu X,Feng M,Yu Q,Hu R,Wang Z

Nuclear envelope-distributed CD147 interacts with and inhibits the transcriptional function of RING1 and promotes melanoma cell motility.
PloS one12(8)e0183689(2017)
Chen J,Peng C,Lei L,Zhang J,Zeng W,Chen X

A High-Density Map for Navigating the Human Polycomb Complexome.
Cell reports17(2)583-595(2016 10)
Hauri S,Comoglio F,Seimiya M,Gerstung M,Glatter T,Hansen K,Aebersold R,Paro R,Gstaiger M,Beisel C

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RING1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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