RAG2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAG2
Entrez Gene ID	5897
Full Name	recombination activating 2
Synonyms	RAG-2
General protein information	Preferred Names recombination activating 2 Names V(D)J recombination-activating protein 2 recombination activating gene 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p12
Summary	This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq, Jul 2008].
Disorder MIM:	179616
Disorder Html:	Severe combined immunodeficiency, B cell-negative, 601457 (3); Omenn syndrome, 603554 (3); Combined cellular and humoral immune defects with granulomas, 233650 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001243785.1	NP_001230714.1	V(D)J recombination-activating protein 2
NM_000536.3	NP_000527.2	V(D)J recombination-activating protein 2
NM_000536.4	NP_000527.2	V(D)J recombination-activating protein 2
NM_001243786.1	NP_001230715.1	V(D)J recombination-activating protein 2
NM_001243785.2	NP_001230714.1	V(D)J recombination-activating protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RAG2	XP_005631162.1
	RAG2	XP_001114734.2
Bos taurus (cattle)	RAG2	NP_001075938.1
Rattus norvegicus (Norway rat)	Rag2	NP_001093998.1
Gallus gallus (chicken)	RAG2	XP_004941607.1
Homo sapiens (human)	RAG2	NP_001230714.1
Mus musculus (house mouse)	Rag2	NP_033046.1
Xenopus tropicalis (tropical clawed frog)	rag2	XP_002937337.2
Pan troglodytes (chimpanzee)	RAG2	XP_521889.1
Danio rerio (zebrafish)	rag2	NP_571460.2

Gene Ontology
Bibliography

Related articles in PubMed

The Conserved ATM Kinase RAG2-S365 Phosphorylation Site Limits Cleavage Events in Individual Cells Independent of Any Repair Defect.
Hewitt SL, Wong JB, Lee JH, Nishana M, Chen H, Coussens M, Arnal SM, Blumenberg LM, Roth DB, Paull TT, Skok JA
Cell reports21(4)979-993(2017 Oct)

The RAG recombinase: Beyond breaking.
Lescale C, Deriano L
Mechanisms of ageing and development165(Pt A)3-9(2017 Jul)

Homozygous deletion of RAG1, RAG2 and 5' region TRAF6 causes severe immune suppression and atypical osteopetrosis.
Weisz Hubshman M, Basel-Vanagaite L, Krauss A, Konen O, Levy Y, Garty BZ, Smirin-Yosef P, Maya I, Lagovsky I, Taub E, Marom D, Gaash D, Shichrur K, Avigad S, Hayman-Manzur L, Villa A, Sobacchi C, Shohat M, Yaniv I, Stein J
Clinical genetics91(6)902-907(2017 Jun)

Estimated disease incidence of RAG1/2 mutations: A?case report and querying the Exome Aggregation Consortium.
Kum?novics A, Lee YN, Close DW, Coonrod EM, Ujhazi B, Chen K, MacArthur DG, Krivan G, Notarangelo LD, Walter JE
The Journal of allergy and clinical immunology139(2)690-692.e3(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We report two siblings with SCID and an atypical phenotype of osteopetrosis (OP). A biallelic microdeletion encompassing the 5' region of TRAF6, RAG1 and RAG2 genes was identified. TRAF6, a tumor necrosis factor receptor-associated family member, plays an important role in T cell signaling and in RANKL-dependent osteoclast differentiation and activation but its role in human OP has not been previously reported
Title: Homozygous deletion of RAG1, RAG2 and 5' region TRAF6 causes severe immune suppression and atypical osteopetrosis.

The show that DNA damage caused by RAG2 activity in pre-B cells was able to downmodulate RAG2 expression and activity, confirming the existence of a negative feedback regulatory mechanism.
Title: The DNA Damage Response Regulates RAG1/2 Expression in Pre-B Cells through ATM-FOXO1 Signaling.

molecular and cellular mechanisms that account for the expanding range of clinical and immunological phenotypes of human RAG deficiency (review)
Title: Human RAG mutations: biochemistry and clinical implications.

gene deficiency results in late onset autoimmune hemolytic anemia
Title: Late Onset Hypomorphic RAG2 Deficiency Presentation with Fatal Vaccine-Strain VZV Infection.

This study reports on the prevalence of RAG1 and RAG2 mutations in ten severe combined immunodeficiency disorder patients in Egypt.
Title: Mutations in Recombination Activating Gene 1 and 2 in patients with severe combined immunodeficiency disorders in Egypt.

The following RAG2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAG2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02396	NM_001243785.2 Latest version!	Homo sapiens recombination activating 2 (RAG2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02396	NM_000536.4 Latest version!	Homo sapiens recombination activating 2 (RAG2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02396	NM_001243786.1 Latest version!	Homo sapiens recombination activating 2 (RAG2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu02396	NM_000536.3	Homo sapiens recombination activating 2 (RAG2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02396	NM_001243785.1	Homo sapiens recombination activating 2 (RAG2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAG2 ( NM_001243785.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230714.1 Homo sapiens recombination activating 2 (RAG2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02396
Clone ID Related Accession (Same CDS sequence)	NM_001243785.1 , NM_000536.3 , NM_000536.4 , NM_001243786.1 , NM_001243785.2
Accession Version	NM_001243785.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-17
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, DB124002.1, AK292664.1, AW058148.1 and BC022397.1. Transcript Variant: This variant (3) includes an alternate exon in the 5' UTR compared to variant 1. Variants 1, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB124002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230714.1
CDS	401..1984
Translation	MSLQMVTVSNNIALIQPGFSLMNFDGQVFFFGQKGWPKRSCPTGVFHLDVKHNHVKLKPTIFSKDSCYLPPLRYPATCTFKGSLESEKHQYIIHGGKTPNNEVSDKIYVMSIVCKNNKKVTFRCTEKDLVGDVPEARYGHSINVVYSRGKSMGVLFGGRSYMPSTHRTTEKWNSVADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSIAKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNMGNGTVFLGIPGDNKQVVSEGFYFYMLKCAEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD

Target ORF information:

RefSeq Version	NM_001243785.1
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 2 (RAG2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243785.1

ORF Insert Sequence:

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG2 ( NM_000536.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000527.2 Homo sapiens recombination activating 2 (RAG2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02396
Clone ID Related Accession (Same CDS sequence)	NM_001243785.1 , NM_000536.3 , NM_000536.4 , NM_001243786.1 , NM_001243785.2
Accession Version	NM_000536.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-17
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, AK292664.1, AW058148.1 and BC022397.1. On May 14, 2010 this sequence version replaced NM_000536.2. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022397.1, AK292664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000527.2
CDS	206..1789
Translation	MSLQMVTVSNNIALIQPGFSLMNFDGQVFFFGQKGWPKRSCPTGVFHLDVKHNHVKLKPTIFSKDSCYLPPLRYPATCTFKGSLESEKHQYIIHGGKTPNNEVSDKIYVMSIVCKNNKKVTFRCTEKDLVGDVPEARYGHSINVVYSRGKSMGVLFGGRSYMPSTHRTTEKWNSVADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSIAKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNMGNGTVFLGIPGDNKQVVSEGFYFYMLKCAEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD

Target ORF information:

RefSeq Version	NM_000536.3
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 2 (RAG2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000536.3

ORF Insert Sequence:

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG2 ( NM_000536.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000527.2 Homo sapiens recombination activating 2 (RAG2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02396
Clone ID Related Accession (Same CDS sequence)	NM_001243785.1 , NM_000536.3 , NM_000536.4 , NM_001243786.1 , NM_001243785.2
Accession Version	NM_000536.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, AK292664.1, AW058148.1 and BC022397.1. On Jun 2, 2019 this sequence version replaced NM_000536.3. Transcript Variant: This variant (1) represents the shortest transcript. Variants 1, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022397.1, AK292664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000527.2
CDS	163..1746
Translation	MSLQMVTVSNNIALIQPGFSLMNFDGQVFFFGQKGWPKRSCPTGVFHLDVKHNHVKLKPTIFSKDSCYLPPLRYPATCTFKGSLESEKHQYIIHGGKTPNNEVSDKIYVMSIVCKNNKKVTFRCTEKDLVGDVPEARYGHSINVVYSRGKSMGVLFGGRSYMPSTHRTTEKWNSVADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSIAKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNMGNGTVFLGIPGDNKQVVSEGFYFYMLKCAEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD

Target ORF information:

RefSeq Version	NM_000536.4
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 2 (RAG2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000536.4

ORF Insert Sequence:

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG2 ( NM_001243786.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230715.1 Homo sapiens recombination activating 2 (RAG2), transcript variant 4, mRNA.

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CloneID	OHu02396
Clone ID Related Accession (Same CDS sequence)	NM_001243785.1 , NM_000536.3 , NM_000536.4 , NM_001243786.1 , NM_001243785.2
Accession Version	NM_001243786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB146077.1, DB143931.1, AC139427.3, AK292664.1, AW058148.1 and BC022397.1. Transcript Variant: This variant (4) includes an alternate exon in the 5' UTR compared to variant 1. Variants 1, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB143931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2159931 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230715.1
CDS	595..2178
Translation	MSLQMVTVSNNIALIQPGFSLMNFDGQVFFFGQKGWPKRSCPTGVFHLDVKHNHVKLKPTIFSKDSCYLPPLRYPATCTFKGSLESEKHQYIIHGGKTPNNEVSDKIYVMSIVCKNNKKVTFRCTEKDLVGDVPEARYGHSINVVYSRGKSMGVLFGGRSYMPSTHRTTEKWNSVADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSIAKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNMGNGTVFLGIPGDNKQVVSEGFYFYMLKCAEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD

Target ORF information:

RefSeq Version	NM_001243786.1
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 2 (RAG2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243786.1

ORF Insert Sequence:

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG2 ( NM_001243785.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230714.1 Homo sapiens recombination activating 2 (RAG2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02396
Clone ID Related Accession (Same CDS sequence)	NM_001243785.1 , NM_000536.3 , NM_000536.4 , NM_001243786.1 , NM_001243785.2
Accession Version	NM_001243785.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB124002.1, AK292664.1, AW058148.1 and BC022397.1. On Jun 2, 2019 this sequence version replaced NM_001243785.1. Transcript Variant: This variant (3) includes an alternate exon in the 5' UTR compared to variant 1. Variants 1, 3 and 4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB124002.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2154529 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230714.1
CDS	358..1941
Translation	MSLQMVTVSNNIALIQPGFSLMNFDGQVFFFGQKGWPKRSCPTGVFHLDVKHNHVKLKPTIFSKDSCYLPPLRYPATCTFKGSLESEKHQYIIHGGKTPNNEVSDKIYVMSIVCKNNKKVTFRCTEKDLVGDVPEARYGHSINVVYSRGKSMGVLFGGRSYMPSTHRTTEKWNSVADCLPCVFLVDFEFGCATSYILPELQDGLSFHVSIAKNDTIYILGGHSLANNIRPANLYRIRVDLPLGSPAVNCTVLPGGISVSSAILTQTNNDEFVIVGGYQLENQKRMICNIISLEDNKIEIREMETPDWTPDIKHSKIWFGSNMGNGTVFLGIPGDNKQVVSEGFYFYMLKCAEDDTNEEQTTFTNSQTSTEDPGDSTPFEDSEEFCFSAEANSFDGDDEFDTYNEDDEEDESETGYWITCCPTCDVDINTWVPFYSTELNKPAMIYCSHGDGHWVHAQCMDLAERTLIHLSAGSNKYYCNEHVEIARALHTPQRVLPLKKPPMKSLRKKGSGKILTPAKKSFLRRLFD

Target ORF information:

RefSeq Version	NM_001243785.2
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 2 (RAG2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243785.2

ORF Insert Sequence:

ATGTCTCTGC AGATGGTAAC AGTCAGTAAT AACATAGCCT TAATTCAGCC AGGCTTCTCA 
CTGATGAATT TTGATGGACA AGTTTTCTTC TTTGGACAAA AAGGCTGGCC CAAAAGATCC 
TGCCCCACTG GAGTTTTCCA TCTGGATGTA AAGCATAACC ATGTCAAACT GAAGCCTACA 
ATTTTCTCTA AGGATTCCTG CTACCTCCCT CCTCTTCGCT ACCCAGCCAC TTGCACATTC 
AAAGGCAGCT TGGAGTCTGA AAAGCATCAA TACATCATCC ATGGAGGGAA AACACCAAAC 
AATGAGGTTT CAGATAAGAT TTATGTCATG TCTATTGTTT GCAAGAACAA CAAAAAGGTT 
ACTTTTCGCT GCACAGAGAA AGACTTGGTA GGAGATGTTC CTGAAGCCAG ATATGGTCAT 
TCCATTAATG TGGTGTACAG CCGAGGGAAA AGTATGGGTG TTCTCTTTGG AGGACGCTCA 
TACATGCCTT CTACCCACAG AACCACAGAA AAATGGAATA GTGTAGCTGA CTGCCTGCCC 
TGTGTTTTCC TGGTGGATTT TGAATTTGGG TGTGCTACAT CATACATTCT TCCAGAACTT 
CAGGATGGGC TATCTTTTCA TGTCTCTATT GCCAAAAATG ACACCATCTA TATTTTAGGA 
GGACATTCAC TTGCCAATAA TATCCGGCCT GCCAACCTGT ACAGAATAAG GGTTGATCTT 
CCCCTGGGTA GCCCAGCTGT GAATTGCACA GTCTTGCCAG GAGGAATCTC TGTCTCCAGT 
GCAATCCTGA CTCAAACTAA CAATGATGAA TTTGTTATTG TTGGTGGCTA TCAGCTTGAA 
AATCAAAAAA GAATGATCTG CAACATCATC TCTTTAGAGG ACAACAAGAT AGAAATTCGT 
GAGATGGAGA CCCCAGATTG GACCCCAGAC ATTAAGCACA GCAAGATATG GTTTGGAAGC 
AACATGGGAA ATGGAACTGT TTTTCTTGGC ATACCAGGAG ACAATAAACA AGTTGTTTCA 
GAAGGATTCT ATTTCTATAT GTTGAAATGT GCTGAAGATG ATACTAATGA AGAGCAGACA 
ACATTCACAA ACAGTCAAAC ATCAACAGAA GATCCAGGGG ATTCCACTCC CTTTGAAGAC 
TCTGAAGAAT TTTGTTTCAG TGCAGAAGCA AATAGTTTTG ATGGTGATGA TGAATTTGAC 
ACCTATAATG AAGATGATGA AGAAGATGAG TCTGAGACAG GCTACTGGAT TACATGCTGC 
CCTACTTGTG ATGTGGATAT CAACACTTGG GTACCATTCT ATTCAACTGA GCTCAACAAA 
CCCGCCATGA TCTACTGCTC TCATGGGGAT GGGCACTGGG TCCATGCTCA GTGCATGGAT 
CTGGCAGAAC GCACACTCAT CCATCTGTCA GCAGGAAGCA ACAAGTATTA CTGCAATGAG 
CATGTGGAGA TAGCAAGAGC TCTACACACT CCCCAAAGAG TCCTACCCTT AAAAAAGCCT 
CCAATGAAAT CCCTCCGTAA AAAAGGTTCT GGAAAAATCT TGACTCCTGC CAAGAAATCC 
TTTCTTAGAA GGTTGTTTGA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Conserved ATM Kinase RAG2-S365 Phosphorylation Site Limits Cleavage Events in Individual Cells Independent of Any Repair Defect.
Cell reports21(4)979-993(2017 Oct)
Hewitt SL,Wong JB,Lee JH,Nishana M,Chen H,Coussens M,Arnal SM,Blumenberg LM,Roth DB,Paull TT,Skok JA

The RAG recombinase: Beyond breaking.
Mechanisms of ageing and development165(Pt A)3-9(2017 Jul)
Lescale C,Deriano L

Homozygous deletion of RAG1, RAG2 and 5' region TRAF6 causes severe immune suppression and atypical osteopetrosis.
Clinical genetics91(6)902-907(2017 Jun)
Weisz Hubshman M,Basel-Vanagaite L,Krauss A,Konen O,Levy Y,Garty BZ,Smirin-Yosef P,Maya I,Lagovsky I,Taub E,Marom D,Gaash D,Shichrur K,Avigad S,Hayman-Manzur L,Villa A,Sobacchi C,Shohat M,Yaniv I,Stein J

Estimated disease incidence of RAG1/2 mutations: A?case report and querying the Exome Aggregation Consortium.
The Journal of allergy and clinical immunology139(2)690-692.e3(2017 Feb)
Kum?novics A,Lee YN,Close DW,Coonrod EM,Ujhazi B,Chen K,MacArthur DG,Krivan G,Notarangelo LD,Walter JE

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RAG2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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