PURA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PURA
Entrez Gene ID	5813
Full Name	purine rich element binding protein A
Synonyms	MRD31,PUR-ALPHA,PUR1,PURALPHA
General protein information	Preferred Names purine rich element binding protein A Names transcriptional activator protein Pur-alpha purine-rich single-stranded DNA-binding protein alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.3
Summary	This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008].
Disorder MIM:	600473
Disorder Html:	Mental retardation, autosomal dominant 31, 616158 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005859.5	NP_005850.1	transcriptional activator protein Pur-alpha
NM_005859.4	NP_005850.1	transcriptional activator protein Pur-alpha

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PURA	XP_527027.1
Homo sapiens (human)	PURA	NP_005850.1
	PURA	XP_001085737.2
Canis lupus familiaris (dog)	PURA	XP_005617331.1
Mus musculus (house mouse)	Pura	NP_033015.1
Gallus gallus (chicken)	PURB	XP_003642090.1
Danio rerio (zebrafish)	pura	NP_001001846.1
Bos taurus (cattle)	PURA	NP_001192363.1
Rattus norvegicus (Norway rat)	Pura	XP_006254682.1
Xenopus tropicalis (tropical clawed frog)	pura	NP_001107341.1

Gene Ontology
Bibliography

Related articles in PubMed

Diagnostic exome sequencing provides a molecular diagnosis for a significant proportion of patients with epilepsy.
Helbig KL, Farwell Hagman KD, Shinde DN, Mroske C, Powis Z, Li S, Tang S, Helbig I
Genetics in medicine : official journal of the American College of Medical Genetics18(9)898-905(2016 Sep)

Potential diagnostic implications of miR-144 overexpression in human oesophageal cancer.
Sharma P, Saraya A, Sharma R
The Indian journal of medical research143(Supplement)S91-S103(2016 May)

Pur-alpha regulates cytoplasmic stress granule dynamics and ameliorates FUS toxicity.
Daigle JG, Krishnamurthy K, Ramesh N, Casci I, Monaghan J, McAvoy K, Godfrey EW, Daniel DC, Johnson EM, Monahan Z, Shewmaker F, Pasinelli P, Pandey UB
Acta neuropathologica131(4)605-20(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Three genes in our epilepsy cohort (COQ4, DNM1, and PURA), accounting for 14% (3/21) of all novel genetic etiologies identified in patients with epilepsy, were subsequently confirmed in independent publications.
Title: Diagnostic exome sequencing provides a molecular diagnosis for a significant proportion of patients with epilepsy.

PURA may be a potential target of miR-144 and observed downregulation of PURA may be caused by increased expression of miR-144. The other predicted target of miR-144 SPRED1, was found to be downregulated in 69 per cent EC tissues as compared to matched distant non-malignant tissues.
Title: Potential diagnostic implications of miR-144 overexpression in human oesophageal cancer.

The results of this study emphasizes the importance of stress granules in ALS pathogenesis and identifies Pur-alpha as a novel regulator of cytoplasmic stress granules dynamics.
Title: Pur-alpha regulates cytoplasmic stress granule dynamics and ameliorates FUS toxicity.

These findings provide definitive evidence for the role of PURA in causing a variable syndrome of neurodevelopmental delay, learning disability, neonatal hypotonia, feeding difficulties, abnormal movements and epilepsy in humans
Title: Whole exome sequencing in family trios reveals de novo mutations in PURA as a cause of severe neurodevelopmental delay and learning disability.

Mutations in PURA cause profound neonatal hypotonia, seizures, and encephalopathy in 5q31.3 microdeletion syndrome.
Title: Mutations in PURA cause profound neonatal hypotonia, seizures, and encephalopathy in 5q31.3 microdeletion syndrome.

The following PURA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PURA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00789	NM_005859.5 Latest version!	Homo sapiens purine rich element binding protein A (PURA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu00789	NM_005859.4	Homo sapiens purine rich element binding protein A (PURA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PURA ( NM_005859.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005850.1 Homo sapiens purine rich element binding protein A (PURA), mRNA.

Price & Availability↑

CloneID	OHu00789
Clone ID Related Accession (Same CDS sequence)	NM_005859.5 , NM_005859.4
Accession Version	NM_005859.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	transcriptional activator protein Pur-alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011379.7, M96684.1 and CK819077.1. On May 17, 2019 this sequence version replaced NM_005859.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: BC036087.1, DRR138524.233110.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331327.5/ ENSP00000332706.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACC GAGACAGCGG CAGCGAGCAG GGTGGTGCGG CGCTGGGTTC GGGCGGCTCC 
CTGGGGCACC CCGGCTCGGG CTCAGGCTCC GGCGGGGGCG GTGGTGGCGG CGGGGGCGGC 
GGCGGCAGTG GCGGCGGCGG CGGCGGGGCC CCAGGGGGGC TGCAGCACGA GACGCAGGAG 
CTGGCCTCCA AGCGGGTGGA CATCCAGAAC AAGCGCTTCT ACCTGGACGT GAAGCAGAAC 
GCCAAGGGCC GCTTCCTGAA GATCGCCGAG GTGGGCGCGG GCGGCAACAA GAGCCGCCTT 
ACTCTCTCCA TGTCAGTGGC CGTGGAGTTC CGCGACTACC TGGGCGACTT CATCGAGCAC 
TACGCGCAGC TGGGCCCCAG CCAGCCGCCG GACCTGGCCC AGGCGCAGGA CGAGCCGCGC 
CGGGCGCTCA AAAGCGAGTT CCTGGTGCGC GAGAACCGCA AGTACTACAT GGATCTCAAG 
GAGAACCAGC GCGGCCGCTT CCTGCGCATC CGCCAGACGG TCAACCGGGG GCCTGGCCTG 
GGCTCCACGC AGGGCCAGAC CATTGCGCTG CCCGCGCAGG GGCTCATCGA GTTCCGTGAC 
GCTCTGGCCA AGCTCATCGA CGACTACGGA GTGGAGGAGG AGCCGGCCGA GCTGCCCGAG 
GGCACCTCCT TGACTGTGGA CAACAAGCGC TTCTTCTTCG ATGTGGGCTC CAACAAGTAC 
GGCGTGTTTA TGCGAGTGAG CGAGGTGAAG CCCACCTATC GCAACTCCAT CACCGTGCCC 
TACAAGGTGT GGGCCAAGTT CGGACACACC TTCTGCAAGT ACTCGGAGGA GATGAAGAAG 
ATTCAAGAGA AGCAGAGGGA GAAGCGGGCT GCCTGTGAGC AGCTTCACCA GCAGCAACAG 
CAGCAGCAGG AGGAGACCGC CGCTGCCACC CTGCTACTGC AGGGTGAGGA AGAAGGGGAA 
GAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005850.1
CDS	74..1042
Translation	MADRDSGSEQGGAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGGAPGGLQHETQELASKRVDIQNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEEMKKIQEKQREKRAACEQLHQQQQQQQEETAAATLLLQGEEEGEED

Target ORF information:

RefSeq Version	NM_005859.5
Organism	Homo sapiens(human)
Definition	Homo sapiens purine rich element binding protein A (PURA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005859.5

ORF Insert Sequence:

ATGGCGGACC GAGACAGCGG CAGCGAGCAG GGTGGTGCGG CGCTGGGTTC GGGCGGCTCC 
CTGGGGCACC CCGGCTCGGG CTCAGGCTCC GGCGGGGGCG GTGGTGGCGG CGGGGGCGGC 
GGCGGCAGTG GCGGCGGCGG CGGCGGGGCC CCAGGGGGGC TGCAGCACGA GACGCAGGAG 
CTGGCCTCCA AGCGGGTGGA CATCCAGAAC AAGCGCTTCT ACCTGGACGT GAAGCAGAAC 
GCCAAGGGCC GCTTCCTGAA GATCGCCGAG GTGGGCGCGG GCGGCAACAA GAGCCGCCTT 
ACTCTCTCCA TGTCAGTGGC CGTGGAGTTC CGCGACTACC TGGGCGACTT CATCGAGCAC 
TACGCGCAGC TGGGCCCCAG CCAGCCGCCG GACCTGGCCC AGGCGCAGGA CGAGCCGCGC 
CGGGCGCTCA AAAGCGAGTT CCTGGTGCGC GAGAACCGCA AGTACTACAT GGATCTCAAG 
GAGAACCAGC GCGGCCGCTT CCTGCGCATC CGCCAGACGG TCAACCGGGG GCCTGGCCTG 
GGCTCCACGC AGGGCCAGAC CATTGCGCTG CCCGCGCAGG GGCTCATCGA GTTCCGTGAC 
GCTCTGGCCA AGCTCATCGA CGACTACGGA GTGGAGGAGG AGCCGGCCGA GCTGCCCGAG 
GGCACCTCCT TGACTGTGGA CAACAAGCGC TTCTTCTTCG ATGTGGGCTC CAACAAGTAC 
GGCGTGTTTA TGCGAGTGAG CGAGGTGAAG CCCACCTATC GCAACTCCAT CACCGTGCCC 
TACAAGGTGT GGGCCAAGTT CGGACACACC TTCTGCAAGT ACTCGGAGGA GATGAAGAAG 
ATTCAAGAGA AGCAGAGGGA GAAGCGGGCT GCCTGTGAGC AGCTTCACCA GCAGCAACAG 
CAGCAGCAGG AGGAGACCGC CGCTGCCACC CTGCTACTGC AGGGTGAGGA AGAAGGGGAA 
GAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PURA ( NM_005859.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005850.1 Homo sapiens purine rich element binding protein A (PURA), mRNA.

Price & Availability↑

CloneID	OHu00789
Clone ID Related Accession (Same CDS sequence)	NM_005859.5 , NM_005859.4
Accession Version	NM_005859.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 969bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	transcriptional activator protein Pur-alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M96684.1, AC011379.7 and CK819077.1. This sequence is a reference standard in the RefSeqGene project. On Dec 28, 2010 this sequence version replaced NM_005859.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGGCGGACC GAGACAGCGG CAGCGAGCAG GGTGGTGCGG CGCTGGGTTC GGGCGGCTCC 
CTGGGGCACC CCGGCTCGGG CTCAGGCTCC GGCGGGGGCG GTGGTGGCGG CGGGGGCGGC 
GGCGGCAGTG GCGGCGGCGG CGGCGGGGCC CCAGGGGGGC TGCAGCACGA GACGCAGGAG 
CTGGCCTCCA AGCGGGTGGA CATCCAGAAC AAGCGCTTCT ACCTGGACGT GAAGCAGAAC 
GCCAAGGGCC GCTTCCTGAA GATCGCCGAG GTGGGCGCGG GCGGCAACAA GAGCCGCCTT 
ACTCTCTCCA TGTCAGTGGC CGTGGAGTTC CGCGACTACC TGGGCGACTT CATCGAGCAC 
TACGCGCAGC TGGGCCCCAG CCAGCCGCCG GACCTGGCCC AGGCGCAGGA CGAGCCGCGC 
CGGGCGCTCA AAAGCGAGTT CCTGGTGCGC GAGAACCGCA AGTACTACAT GGATCTCAAG 
GAGAACCAGC GCGGCCGCTT CCTGCGCATC CGCCAGACGG TCAACCGGGG GCCTGGCCTG 
GGCTCCACGC AGGGCCAGAC CATTGCGCTG CCCGCGCAGG GGCTCATCGA GTTCCGTGAC 
GCTCTGGCCA AGCTCATCGA CGACTACGGA GTGGAGGAGG AGCCGGCCGA GCTGCCCGAG 
GGCACCTCCT TGACTGTGGA CAACAAGCGC TTCTTCTTCG ATGTGGGCTC CAACAAGTAC 
GGCGTGTTTA TGCGAGTGAG CGAGGTGAAG CCCACCTATC GCAACTCCAT CACCGTGCCC 
TACAAGGTGT GGGCCAAGTT CGGACACACC TTCTGCAAGT ACTCGGAGGA GATGAAGAAG 
ATTCAAGAGA AGCAGAGGGA GAAGCGGGCT GCCTGTGAGC AGCTTCACCA GCAGCAACAG 
CAGCAGCAGG AGGAGACCGC CGCTGCCACC CTGCTACTGC AGGGTGAGGA AGAAGGGGAA 
GAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005850.1
CDS	60..1028
Translation	MADRDSGSEQGGAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGGAPGGLQHETQELASKRVDIQNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEEMKKIQEKQREKRAACEQLHQQQQQQQEETAAATLLLQGEEEGEED

Target ORF information:

RefSeq Version	NM_005859.4
Organism	Homo sapiens(human)
Definition	Homo sapiens purine rich element binding protein A (PURA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005859.4

ORF Insert Sequence:

ATGGCGGACC GAGACAGCGG CAGCGAGCAG GGTGGTGCGG CGCTGGGTTC GGGCGGCTCC 
CTGGGGCACC CCGGCTCGGG CTCAGGCTCC GGCGGGGGCG GTGGTGGCGG CGGGGGCGGC 
GGCGGCAGTG GCGGCGGCGG CGGCGGGGCC CCAGGGGGGC TGCAGCACGA GACGCAGGAG 
CTGGCCTCCA AGCGGGTGGA CATCCAGAAC AAGCGCTTCT ACCTGGACGT GAAGCAGAAC 
GCCAAGGGCC GCTTCCTGAA GATCGCCGAG GTGGGCGCGG GCGGCAACAA GAGCCGCCTT 
ACTCTCTCCA TGTCAGTGGC CGTGGAGTTC CGCGACTACC TGGGCGACTT CATCGAGCAC 
TACGCGCAGC TGGGCCCCAG CCAGCCGCCG GACCTGGCCC AGGCGCAGGA CGAGCCGCGC 
CGGGCGCTCA AAAGCGAGTT CCTGGTGCGC GAGAACCGCA AGTACTACAT GGATCTCAAG 
GAGAACCAGC GCGGCCGCTT CCTGCGCATC CGCCAGACGG TCAACCGGGG GCCTGGCCTG 
GGCTCCACGC AGGGCCAGAC CATTGCGCTG CCCGCGCAGG GGCTCATCGA GTTCCGTGAC 
GCTCTGGCCA AGCTCATCGA CGACTACGGA GTGGAGGAGG AGCCGGCCGA GCTGCCCGAG 
GGCACCTCCT TGACTGTGGA CAACAAGCGC TTCTTCTTCG ATGTGGGCTC CAACAAGTAC 
GGCGTGTTTA TGCGAGTGAG CGAGGTGAAG CCCACCTATC GCAACTCCAT CACCGTGCCC 
TACAAGGTGT GGGCCAAGTT CGGACACACC TTCTGCAAGT ACTCGGAGGA GATGAAGAAG 
ATTCAAGAGA AGCAGAGGGA GAAGCGGGCT GCCTGTGAGC AGCTTCACCA GCAGCAACAG 
CAGCAGCAGG AGGAGACCGC CGCTGCCACC CTGCTACTGC AGGGTGAGGA AGAAGGGGAA 
GAAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diagnostic exome sequencing provides a molecular diagnosis for a significant proportion of patients with epilepsy.
Genetics in medicine : official journal of the American College of Medical Genetics18(9)898-905(2016 Sep)
Helbig KL,Farwell Hagman KD,Shinde DN,Mroske C,Powis Z,Li S,Tang S,Helbig I

Potential diagnostic implications of miR-144 overexpression in human oesophageal cancer.
The Indian journal of medical research143(Supplement)S91-S103(2016 May)
Sharma P,Saraya A,Sharma R

Pur-alpha regulates cytoplasmic stress granule dynamics and ameliorates FUS toxicity.
Acta neuropathologica131(4)605-20(2016 Apr)
Daigle JG,Krishnamurthy K,Ramesh N,Casci I,Monaghan J,McAvoy K,Godfrey EW,Daniel DC,Johnson EM,Monahan Z,Shewmaker F,Pasinelli P,Pandey UB

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PURA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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