KLHL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KLHL1
Entrez Gene ID	57626
Full Name	kelch like family member 1
Synonyms	MRP2
General protein information	Preferred Names kelch like family member 1 Names kelch-like protein 1 Mayven-related protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q21.33
Summary	The KLHL1 protein belongs to a family of actin-organizing proteins related to Drosophila Kelch (Nemes et al., 2000 [PubMed 10888605]).[supplied by OMIM, Feb 2010].
Disorder MIM:	605332

mRNA and Protein(s)

mRNA	Protein	Name
XM_017020678.1	XP_016876167.1	kelch-like protein 1 isoform X1
XM_017020678.2	XP_016876167.1	kelch-like protein 1 isoform X1
NM_001286725.1	NP_001273654.1	kelch-like protein 1 isoform 2
NM_020866.2	NP_065917.1	kelch-like protein 1 isoform 1
NM_020866.3	NP_065917.1	kelch-like protein 1 isoform 1
XM_017020679.1	XP_016876168.1	kelch-like protein 1 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Klhl1	NP_444335.2
Gallus gallus (chicken)	KLHL1	XP_416994.2
Homo sapiens (human)	KLHL1	NP_065917.1
Pan troglodytes (chimpanzee)	KLHL1	XP_509677.2
Canis lupus familiaris (dog)	KLHL1	XP_542606.4
Bos taurus (cattle)	KLHL1	NP_001179984.1
Rattus norvegicus (Norway rat)	Klhl1	NP_001099524.1

Gene Ontology
Bibliography

Related articles in PubMed

Update on the Kelch-like (KLHL) gene family.
Dhanoa BS, Cogliati T, Satish AG, Bruford EA, Friedman JS
Human genomics713(2013 May)

Genome-wide contribution of genotype by environment interaction to variation of diabetes-related traits.
Zheng JS, Arnett DK, Lee YC, Shen J, Parnell LD, Smith CE, Richardson K, Li D, Borecki IB, Ordov?s JM, Lai CQ
PloS one8(10)e77442(2013)

Genome-wide association study of d-amphetamine response in healthy volunteers identifies putative associations, including cadherin 13 (CDH13).
Hart AB, Engelhardt BE, Wardle MC, Sokoloff G, Stephens M, de Wit H, Palmer AA
PloS one7(8)e42646(2012)

Influence of MRP2 on MPA pharmacokinetics in renal transplant recipients-results of the Pharmacogenomic Substudy within the Symphony Study.
Lloberas N, Torras J, Cruzado JM, Andreu F, Oppenheimer F, S?nchez-Plumed J, Gentil M?, Brunet M, Ekberg H, Griny? JM,
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association26(11)3784-93(2011 Nov)

T-type current modulation by the actin-binding protein Kelch-like 1.
Aromolaran KA, Benzow KA, Cribbs LL, Koob MD, Piedras-Renter?a ES
American journal of physiology. Cell physiology298(6)C1353-62(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Renal transplant recipients T carriers of C24T MRP2 with macrolides treatment were associated with reduced mycophenolic acid area under curve in steady-state conditions
Title: Influence of MRP2 on MPA pharmacokinetics in renal transplant recipients-results of the Pharmacogenomic Substudy within the Symphony Study.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

KLHL1 interacts with Cav3.2 and modulates T-type calcium currents by increasing current deactivation kinetics and current density and calcium influx.
Title: T-type current modulation by the actin-binding protein Kelch-like 1.

KLHL1 was expressed in human brain and kidney, but was also in other tissues, such as prostate gland, small intestine, colon, testis (but not the ovaries), liver and pancreas.
Title: SCA8 mRNA expression suggests an antisense regulation of KLHL1 and correlates to SCA8 pathology.

modulatory effect of KLHL1 on the P/Q-type calcium channel function, suggesting a possible novel role for KLHL1 in cellular excitability.
Title: The Kelch-like protein 1 modulates P/Q-type calcium current density.

The following KLHL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KLHL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25041	NM_020866.3 Latest version!	Homo sapiens kelch like family member 1 (KLHL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu25282	NM_001286725.1 Latest version!	Homo sapiens kelch like family member 1 (KLHL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu84597	XM_017020678.2 Latest version!	Homo sapiens kelch like family member 1 (KLHL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu84598	XM_017020679.1 Latest version!	Homo sapiens kelch like family member 1 (KLHL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu84597	XM_017020678.1	Homo sapiens kelch like family member 1 (KLHL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu25041	NM_020866.2	Homo sapiens kelch like family member 1 (KLHL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KLHL1 ( NM_020866.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065917.1 Homo sapiens kelch like family member 1 (KLHL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25041
Clone ID Related Accession (Same CDS sequence)	NM_020866.2 , NM_020866.3
Accession Version	NM_020866.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	kelch-like protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK291435.1, AF252283.1 and BC022460.1. On Jan 24, 2009 this sequence version replaced NM_020866.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF252283.1, AB040923.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGGCT CTGGGCGAAA AGACTTCGAT GTGAAGCACA TTCTGCGACT CCGCTGGAAA 
CTCTTCAGCC ACCCGTCTCC TTCCACCGGC GGCCCGGCGG GGGGAGGCTG CCTGCAACAG 
GACGGCAGTG GCAGCTTTGA GCACTGGGGA CCCAGCCAGA GTCGCCTGCT CAAAAGCCAA 
GAGAGAAGCG GTGTGAGCAC TTTCTGGAAG AAGCCTTCCT CCTCTTCCTC TTCATCGTCC 
TCCCCGTCCT CTTCCTCCTC TTCCTTCAAT CCGCTGAATG GCACCCTGCT TCCAGTTGCC 
ACGAGGCTGC AGCAAGGGGC TCCTGGGCAG GGCACTCAGC AGCCAGCCAG GACTCTCTTC 
TACGTGGAGT CACTAGAGGA GGAGGTGGTG CCAGGCATGG ACTTTCCTGG ACCACACGAA 
AAAGGGCTGG TTCTGCAAGA GCTCAAAGTG GAGCCAGACA ACTCTAGCCA GGCAACAGGT 
GAAGGATGTG GACACAGGCT GTCATCAACC GGCCATTCAA TGACACCTCA AAGTGATTTG 
GACTCCAGTA GCTCTGAAGA ATTCTATCAA GCTGTTCATC ATGCTGAGCA AACCTTCAGA 
AAGATGGAAA GTTATTTGAA GCAGCAGCAA CTTTGTGATG TTATCCTGAT TGTTGGGAAC 
CGAAAGATAC CTGCACATAG GCTTGTTCTG AGTTCAGTCT CCGACTATTT TGCGGCCATG 
TTTACAAGTG ATGTTTGTGA AGCCAAGCAA GAGGAGATCA AAATGGAAGG CATAGACCCC 
AATGCTCTCT GGGACCTTGT CCAATTTGCA TATACAGGCT GCTTGGAATT AAAAGAGGAC 
ACCATTGAGA ACCTTCTTGC TGCAGCGTGC CTTCTTCAGC TTCCACAGGT GGTGGAAGTG 
TGCTGCCACT TCCTCATGAA GCTTTTGCAT CCATCTAACT GTTTAGGAAT TCGAGCCTTC 
GCAGATGCTC AAGGATGCAT TGAGTTAATG AAGGTGGCCC ACAGCTACAC AATGGAAAAC 
ATAATGGAAG TTATCAGAAA TCAAGAGTTT TTACTCCTTC CAGCTGAGGA GCTCCATAAA 
CTACTGGCCA GTGATGATGT CAATGTTCCT GATGAAGAAA CCATCTTCCA TGCATTGATG 
ATGTGGGTCA AGTATGACAT GCAGAGTAGA TGCAATGACC TGAGCATGCT TCTTGCCTTT 
ATAAGACTGC CACTGCTTCC ACCACAGATA TTGGCTGACC TAGAAAATCA TGCATTATTT 
AAGAATGATC TGGAATGTCA AAAGCTGATT CTAGAAGCAA TGAAATACCA TCTATTGCCA 
GAAAGAAGAA CTTTAATGCA AAGTCCGAGA ACTAAACCCA GAAAATCTAC AGTCGGAACT 
TTGTATGCTG TAGGAGGAAT GGATAACAAC AAAGGAGCTA CAACTATAGA GAAATATGAT 
CTGAGAACAA ATCTGTGGAT CCAGGCAGGG ATGATGAATG GCAGAAGGCT GCAGTTTGGT 
GTGGCTGTTA TTGATGACAA ACTCTTTGTA ATTGGAGGTC GAGATGGCTT AAAGACATTG 
AACACTGTTG AATGTTACAA TCCCAAAACC AAGACATGGA CTGTCTTACC ACCAATGTCA 
ACACACAGAC ATGGTCTAGG TGTAACAGTA CTTGAAGGCC CTATTTATGC TGTGGGAGGC 
CATGATGGCT GGAGCTATCT GAATACAGTG GAAAGGTGGG ATCCACAGAG TCAACAATGG 
ACATTTGTAG CCAGTATGTC AATTGCTCGG AGCACAGTTG GTGTAGCAGC ATTGAATGGC 
AAGTTGTATT CAGTTGGAGG TCGTGATGGA AGTTCCTGTT TGAGTTCAAT GGAATATTAT 
GATCCTCATA CAAATAAGTG GAACATGTGT GCTCCCATGT GTAAGAGGAG AGGGGGTGTC 
GGAGTGGCCA CATGTGACGG TTTTCTTTAT GCAGTAGGAG GTCATGATGC TCCTGCTTCA 
AATCACTGTT CCCGGCTACT GGATTATGTA GAAAGATATG ATCCCAAAAC AGACACTTGG 
ACCATGGTGG CTCCTTTGAG TATGCCCAGA GATGCTGTTG GGGTCTGTCT CCTTGGTGAC 
AGATTATATG CTGTTGGTGG CTATGATGGA CAGACATACC TCAACACTAT GGAATCCTAT 
GACCCACAAA CTAATGAGTG GACACAGATG GCTTCCTTGA ATATTGGGAG AGCAGGTGCC 
TGTGTGGTAG TCATCAAGCA ACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065917.1
CDS	795..3041
Translation	MSGSGRKDFDVKHILRLRWKLFSHPSPSTGGPAGGGCLQQDGSGSFEHWGPSQSRLLKSQERSGVSTFWKKPSSSSSSSSSPSSSSSSFNPLNGTLLPVATRLQQGAPGQGTQQPARTLFYVESLEEEVVPGMDFPGPHEKGLVLQELKVEPDNSSQATGEGCGHRLSSTGHSMTPQSDLDSSSSEEFYQAVHHAEQTFRKMESYLKQQQLCDVILIVGNRKIPAHRLVLSSVSDYFAAMFTSDVCEAKQEEIKMEGIDPNALWDLVQFAYTGCLELKEDTIENLLAAACLLQLPQVVEVCCHFLMKLLHPSNCLGIRAFADAQGCIELMKVAHSYTMENIMEVIRNQEFLLLPAEELHKLLASDDVNVPDEETIFHALMMWVKYDMQSRCNDLSMLLAFIRLPLLPPQILADLENHALFKNDLECQKLILEAMKYHLLPERRTLMQSPRTKPRKSTVGTLYAVGGMDNNKGATTIEKYDLRTNLWIQAGMMNGRRLQFGVAVIDDKLFVIGGRDGLKTLNTVECYNPKTKTWTVLPPMSTHRHGLGVTVLEGPIYAVGGHDGWSYLNTVERWDPQSQQWTFVASMSIARSTVGVAALNGKLYSVGGRDGSSCLSSMEYYDPHTNKWNMCAPMCKRRGGVGVATCDGFLYAVGGHDAPASNHCSRLLDYVERYDPKTDTWTMVAPLSMPRDAVGVCLLGDRLYAVGGYDGQTYLNTMESYDPQTNEWTQMASLNIGRAGACVVVIKQP

Target ORF information:

RefSeq Version	NM_020866.2
Organism	Homo sapiens(human)
Definition	Homo sapiens kelch like family member 1 (KLHL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020866.2

ORF Insert Sequence:

ATGTCAGGCT CTGGGCGAAA AGACTTCGAT GTGAAGCACA TTCTGCGACT CCGCTGGAAA 
CTCTTCAGCC ACCCGTCTCC TTCCACCGGC GGCCCGGCGG GGGGAGGCTG CCTGCAACAG 
GACGGCAGTG GCAGCTTTGA GCACTGGGGA CCCAGCCAGA GTCGCCTGCT CAAAAGCCAA 
GAGAGAAGCG GTGTGAGCAC TTTCTGGAAG AAGCCTTCCT CCTCTTCCTC TTCATCGTCC 
TCCCCGTCCT CTTCCTCCTC TTCCTTCAAT CCGCTGAATG GCACCCTGCT TCCAGTTGCC 
ACGAGGCTGC AGCAAGGGGC TCCTGGGCAG GGCACTCAGC AGCCAGCCAG GACTCTCTTC 
TACGTGGAGT CACTAGAGGA GGAGGTGGTG CCAGGCATGG ACTTTCCTGG ACCACACGAA 
AAAGGGCTGG TTCTGCAAGA GCTCAAAGTG GAGCCAGACA ACTCTAGCCA GGCAACAGGT 
GAAGGATGTG GACACAGGCT GTCATCAACC GGCCATTCAA TGACACCTCA AAGTGATTTG 
GACTCCAGTA GCTCTGAAGA ATTCTATCAA GCTGTTCATC ATGCTGAGCA AACCTTCAGA 
AAGATGGAAA GTTATTTGAA GCAGCAGCAA CTTTGTGATG TTATCCTGAT TGTTGGGAAC 
CGAAAGATAC CTGCACATAG GCTTGTTCTG AGTTCAGTCT CCGACTATTT TGCGGCCATG 
TTTACAAGTG ATGTTTGTGA AGCCAAGCAA GAGGAGATCA AAATGGAAGG CATAGACCCC 
AATGCTCTCT GGGACCTTGT CCAATTTGCA TATACAGGCT GCTTGGAATT AAAAGAGGAC 
ACCATTGAGA ACCTTCTTGC TGCAGCGTGC CTTCTTCAGC TTCCACAGGT GGTGGAAGTG 
TGCTGCCACT TCCTCATGAA GCTTTTGCAT CCATCTAACT GTTTAGGAAT TCGAGCCTTC 
GCAGATGCTC AAGGATGCAT TGAGTTAATG AAGGTGGCCC ACAGCTACAC AATGGAAAAC 
ATAATGGAAG TTATCAGAAA TCAAGAGTTT TTACTCCTTC CAGCTGAGGA GCTCCATAAA 
CTACTGGCCA GTGATGATGT CAATGTTCCT GATGAAGAAA CCATCTTCCA TGCATTGATG 
ATGTGGGTCA AGTATGACAT GCAGAGTAGA TGCAATGACC TGAGCATGCT TCTTGCCTTT 
ATAAGACTGC CACTGCTTCC ACCACAGATA TTGGCTGACC TAGAAAATCA TGCATTATTT 
AAGAATGATC TGGAATGTCA AAAGCTGATT CTAGAAGCAA TGAAATACCA TCTATTGCCA 
GAAAGAAGAA CTTTAATGCA AAGTCCGAGA ACTAAACCCA GAAAATCTAC AGTCGGAACT 
TTGTATGCTG TAGGAGGAAT GGATAACAAC AAAGGAGCTA CAACTATAGA GAAATATGAT 
CTGAGAACAA ATCTGTGGAT CCAGGCAGGG ATGATGAATG GCAGAAGGCT GCAGTTTGGT 
GTGGCTGTTA TTGATGACAA ACTCTTTGTA ATTGGAGGTC GAGATGGCTT AAAGACATTG 
AACACTGTTG AATGTTACAA TCCCAAAACC AAGACATGGA CTGTCTTACC ACCAATGTCA 
ACACACAGAC ATGGTCTAGG TGTAACAGTA CTTGAAGGCC CTATTTATGC TGTGGGAGGC 
CATGATGGCT GGAGCTATCT GAATACAGTG GAAAGGTGGG ATCCACAGAG TCAACAATGG 
ACATTTGTAG CCAGTATGTC AATTGCTCGG AGCACAGTTG GTGTAGCAGC ATTGAATGGC 
AAGTTGTATT CAGTTGGAGG TCGTGATGGA AGTTCCTGTT TGAGTTCAAT GGAATATTAT 
GATCCTCATA CAAATAAGTG GAACATGTGT GCTCCCATGT GTAAGAGGAG AGGGGGTGTC 
GGAGTGGCCA CATGTGACGG TTTTCTTTAT GCAGTAGGAG GTCATGATGC TCCTGCTTCA 
AATCACTGTT CCCGGCTACT GGATTATGTA GAAAGATATG ATCCCAAAAC AGACACTTGG 
ACCATGGTGG CTCCTTTGAG TATGCCCAGA GATGCTGTTG GGGTCTGTCT CCTTGGTGAC 
AGATTATATG CTGTTGGTGG CTATGATGGA CAGACATACC TCAACACTAT GGAATCCTAT 
GACCCACAAA CTAATGAGTG GACACAGATG GCTTCCTTGA ATATTGGGAG AGCAGGTGCC 
TGTGTGGTAG TCATCAAGCA ACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KLHL1 ( NM_020866.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065917.1 Homo sapiens kelch like family member 1 (KLHL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25041
Clone ID Related Accession (Same CDS sequence)	NM_020866.2 , NM_020866.3
Accession Version	NM_020866.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	kelch-like protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK291435.1, AF252283.1 and BC022460.1. On Nov 23, 2018 this sequence version replaced NM_020866.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC022460.1, AB040923.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465404 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000377844.9/ ENSP00000367075.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCAGGCT CTGGGCGAAA AGACTTCGAT GTGAAGCACA TTCTGCGACT CCGCTGGAAA 
CTCTTCAGCC ACCCGTCTCC TTCCACCGGC GGCCCGGCGG GGGGAGGCTG CCTGCAACAG 
GACGGCAGTG GCAGCTTTGA GCACTGGGGA CCCAGCCAGA GTCGCCTGCT CAAAAGCCAA 
GAGAGAAGCG GTGTGAGCAC TTTCTGGAAG AAGCCTTCCT CCTCTTCCTC TTCATCGTCC 
TCCCCGTCCT CTTCCTCCTC TTCCTTCAAT CCGCTGAATG GCACCCTGCT TCCAGTTGCC 
ACGAGGCTGC AGCAAGGGGC TCCTGGGCAG GGCACTCAGC AGCCAGCCAG GACTCTCTTC 
TACGTGGAGT CACTAGAGGA GGAGGTGGTG CCAGGCATGG ACTTTCCTGG ACCACACGAA 
AAAGGGCTGG TTCTGCAAGA GCTCAAAGTG GAGCCAGACA ACTCTAGCCA GGCAACAGGT 
GAAGGATGTG GACACAGGCT GTCATCAACC GGCCATTCAA TGACACCTCA AAGTGATTTG 
GACTCCAGTA GCTCTGAAGA ATTCTATCAA GCTGTTCATC ATGCTGAGCA AACCTTCAGA 
AAGATGGAAA GTTATTTGAA GCAGCAGCAA CTTTGTGATG TTATCCTGAT TGTTGGGAAC 
CGAAAGATAC CTGCACATAG GCTTGTTCTG AGTTCAGTCT CCGACTATTT TGCGGCCATG 
TTTACAAGTG ATGTTTGTGA AGCCAAGCAA GAGGAGATCA AAATGGAAGG CATAGACCCC 
AATGCTCTCT GGGACCTTGT CCAATTTGCA TATACAGGCT GCTTGGAATT AAAAGAGGAC 
ACCATTGAGA ACCTTCTTGC TGCAGCGTGC CTTCTTCAGC TTCCACAGGT GGTGGAAGTG 
TGCTGCCACT TCCTCATGAA GCTTTTGCAT CCATCTAACT GTTTAGGAAT TCGAGCCTTC 
GCAGATGCTC AAGGATGCAT TGAGTTAATG AAGGTGGCCC ACAGCTACAC AATGGAAAAC 
ATAATGGAAG TTATCAGAAA TCAAGAGTTT TTACTCCTTC CAGCTGAGGA GCTCCATAAA 
CTACTGGCCA GTGATGATGT CAATGTTCCT GATGAAGAAA CCATCTTCCA TGCATTGATG 
ATGTGGGTCA AGTATGACAT GCAGAGTAGA TGCAATGACC TGAGCATGCT TCTTGCCTTT 
ATAAGACTGC CACTGCTTCC ACCACAGATA TTGGCTGACC TAGAAAATCA TGCATTATTT 
AAGAATGATC TGGAATGTCA AAAGCTGATT CTAGAAGCAA TGAAATACCA TCTATTGCCA 
GAAAGAAGAA CTTTAATGCA AAGTCCGAGA ACTAAACCCA GAAAATCTAC AGTCGGAACT 
TTGTATGCTG TAGGAGGAAT GGATAACAAC AAAGGAGCTA CAACTATAGA GAAATATGAT 
CTGAGAACAA ATCTGTGGAT CCAGGCAGGG ATGATGAATG GCAGAAGGCT GCAGTTTGGT 
GTGGCTGTTA TTGATGACAA ACTCTTTGTA ATTGGAGGTC GAGATGGCTT AAAGACATTG 
AACACTGTTG AATGTTACAA TCCCAAAACC AAGACATGGA CTGTCTTACC ACCAATGTCA 
ACACACAGAC ATGGTCTAGG TGTAACAGTA CTTGAAGGCC CTATTTATGC TGTGGGAGGC 
CATGATGGCT GGAGCTATCT GAATACAGTG GAAAGGTGGG ATCCACAGAG TCAACAATGG 
ACATTTGTAG CCAGTATGTC AATTGCTCGG AGCACAGTTG GTGTAGCAGC ATTGAATGGC 
AAGTTGTATT CAGTTGGAGG TCGTGATGGA AGTTCCTGTT TGAGTTCAAT GGAATATTAT 
GATCCTCATA CAAATAAGTG GAACATGTGT GCTCCCATGT GTAAGAGGAG AGGGGGTGTC 
GGAGTGGCCA CATGTGACGG TTTTCTTTAT GCAGTAGGAG GTCATGATGC TCCTGCTTCA 
AATCACTGTT CCCGGCTACT GGATTATGTA GAAAGATATG ATCCCAAAAC AGACACTTGG 
ACCATGGTGG CTCCTTTGAG TATGCCCAGA GATGCTGTTG GGGTCTGTCT CCTTGGTGAC 
AGATTATATG CTGTTGGTGG CTATGATGGA CAGACATACC TCAACACTAT GGAATCCTAT 
GACCCACAAA CTAATGAGTG GACACAGATG GCTTCCTTGA ATATTGGGAG AGCAGGTGCC 
TGTGTGGTAG TCATCAAGCA ACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065917.1
CDS	754..3000
Translation	MSGSGRKDFDVKHILRLRWKLFSHPSPSTGGPAGGGCLQQDGSGSFEHWGPSQSRLLKSQERSGVSTFWKKPSSSSSSSSSPSSSSSSFNPLNGTLLPVATRLQQGAPGQGTQQPARTLFYVESLEEEVVPGMDFPGPHEKGLVLQELKVEPDNSSQATGEGCGHRLSSTGHSMTPQSDLDSSSSEEFYQAVHHAEQTFRKMESYLKQQQLCDVILIVGNRKIPAHRLVLSSVSDYFAAMFTSDVCEAKQEEIKMEGIDPNALWDLVQFAYTGCLELKEDTIENLLAAACLLQLPQVVEVCCHFLMKLLHPSNCLGIRAFADAQGCIELMKVAHSYTMENIMEVIRNQEFLLLPAEELHKLLASDDVNVPDEETIFHALMMWVKYDMQSRCNDLSMLLAFIRLPLLPPQILADLENHALFKNDLECQKLILEAMKYHLLPERRTLMQSPRTKPRKSTVGTLYAVGGMDNNKGATTIEKYDLRTNLWIQAGMMNGRRLQFGVAVIDDKLFVIGGRDGLKTLNTVECYNPKTKTWTVLPPMSTHRHGLGVTVLEGPIYAVGGHDGWSYLNTVERWDPQSQQWTFVASMSIARSTVGVAALNGKLYSVGGRDGSSCLSSMEYYDPHTNKWNMCAPMCKRRGGVGVATCDGFLYAVGGHDAPASNHCSRLLDYVERYDPKTDTWTMVAPLSMPRDAVGVCLLGDRLYAVGGYDGQTYLNTMESYDPQTNEWTQMASLNIGRAGACVVVIKQP

Target ORF information:

RefSeq Version	NM_020866.3
Organism	Homo sapiens(human)
Definition	Homo sapiens kelch like family member 1 (KLHL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020866.3

ORF Insert Sequence:

ATGTCAGGCT CTGGGCGAAA AGACTTCGAT GTGAAGCACA TTCTGCGACT CCGCTGGAAA 
CTCTTCAGCC ACCCGTCTCC TTCCACCGGC GGCCCGGCGG GGGGAGGCTG CCTGCAACAG 
GACGGCAGTG GCAGCTTTGA GCACTGGGGA CCCAGCCAGA GTCGCCTGCT CAAAAGCCAA 
GAGAGAAGCG GTGTGAGCAC TTTCTGGAAG AAGCCTTCCT CCTCTTCCTC TTCATCGTCC 
TCCCCGTCCT CTTCCTCCTC TTCCTTCAAT CCGCTGAATG GCACCCTGCT TCCAGTTGCC 
ACGAGGCTGC AGCAAGGGGC TCCTGGGCAG GGCACTCAGC AGCCAGCCAG GACTCTCTTC 
TACGTGGAGT CACTAGAGGA GGAGGTGGTG CCAGGCATGG ACTTTCCTGG ACCACACGAA 
AAAGGGCTGG TTCTGCAAGA GCTCAAAGTG GAGCCAGACA ACTCTAGCCA GGCAACAGGT 
GAAGGATGTG GACACAGGCT GTCATCAACC GGCCATTCAA TGACACCTCA AAGTGATTTG 
GACTCCAGTA GCTCTGAAGA ATTCTATCAA GCTGTTCATC ATGCTGAGCA AACCTTCAGA 
AAGATGGAAA GTTATTTGAA GCAGCAGCAA CTTTGTGATG TTATCCTGAT TGTTGGGAAC 
CGAAAGATAC CTGCACATAG GCTTGTTCTG AGTTCAGTCT CCGACTATTT TGCGGCCATG 
TTTACAAGTG ATGTTTGTGA AGCCAAGCAA GAGGAGATCA AAATGGAAGG CATAGACCCC 
AATGCTCTCT GGGACCTTGT CCAATTTGCA TATACAGGCT GCTTGGAATT AAAAGAGGAC 
ACCATTGAGA ACCTTCTTGC TGCAGCGTGC CTTCTTCAGC TTCCACAGGT GGTGGAAGTG 
TGCTGCCACT TCCTCATGAA GCTTTTGCAT CCATCTAACT GTTTAGGAAT TCGAGCCTTC 
GCAGATGCTC AAGGATGCAT TGAGTTAATG AAGGTGGCCC ACAGCTACAC AATGGAAAAC 
ATAATGGAAG TTATCAGAAA TCAAGAGTTT TTACTCCTTC CAGCTGAGGA GCTCCATAAA 
CTACTGGCCA GTGATGATGT CAATGTTCCT GATGAAGAAA CCATCTTCCA TGCATTGATG 
ATGTGGGTCA AGTATGACAT GCAGAGTAGA TGCAATGACC TGAGCATGCT TCTTGCCTTT 
ATAAGACTGC CACTGCTTCC ACCACAGATA TTGGCTGACC TAGAAAATCA TGCATTATTT 
AAGAATGATC TGGAATGTCA AAAGCTGATT CTAGAAGCAA TGAAATACCA TCTATTGCCA 
GAAAGAAGAA CTTTAATGCA AAGTCCGAGA ACTAAACCCA GAAAATCTAC AGTCGGAACT 
TTGTATGCTG TAGGAGGAAT GGATAACAAC AAAGGAGCTA CAACTATAGA GAAATATGAT 
CTGAGAACAA ATCTGTGGAT CCAGGCAGGG ATGATGAATG GCAGAAGGCT GCAGTTTGGT 
GTGGCTGTTA TTGATGACAA ACTCTTTGTA ATTGGAGGTC GAGATGGCTT AAAGACATTG 
AACACTGTTG AATGTTACAA TCCCAAAACC AAGACATGGA CTGTCTTACC ACCAATGTCA 
ACACACAGAC ATGGTCTAGG TGTAACAGTA CTTGAAGGCC CTATTTATGC TGTGGGAGGC 
CATGATGGCT GGAGCTATCT GAATACAGTG GAAAGGTGGG ATCCACAGAG TCAACAATGG 
ACATTTGTAG CCAGTATGTC AATTGCTCGG AGCACAGTTG GTGTAGCAGC ATTGAATGGC 
AAGTTGTATT CAGTTGGAGG TCGTGATGGA AGTTCCTGTT TGAGTTCAAT GGAATATTAT 
GATCCTCATA CAAATAAGTG GAACATGTGT GCTCCCATGT GTAAGAGGAG AGGGGGTGTC 
GGAGTGGCCA CATGTGACGG TTTTCTTTAT GCAGTAGGAG GTCATGATGC TCCTGCTTCA 
AATCACTGTT CCCGGCTACT GGATTATGTA GAAAGATATG ATCCCAAAAC AGACACTTGG 
ACCATGGTGG CTCCTTTGAG TATGCCCAGA GATGCTGTTG GGGTCTGTCT CCTTGGTGAC 
AGATTATATG CTGTTGGTGG CTATGATGGA CAGACATACC TCAACACTAT GGAATCCTAT 
GACCCACAAA CTAATGAGTG GACACAGATG GCTTCCTTGA ATATTGGGAG AGCAGGTGCC 
TGTGTGGTAG TCATCAAGCA ACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KLHL1 ( NM_001286725.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273654.1 Homo sapiens kelch like family member 1 (KLHL1), transcript variant 2, mRNA.

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CloneID	OHu25282
Clone ID Related Accession (Same CDS sequence)	NM_001286725.1
Accession Version	NM_001286725.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Homo sapiens(human)
Product	kelch-like protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK291435.1, AF252283.1, AK295939.1 and BC022460.1. On Nov 14, 2013 this sequence version replaced XM_005266484.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.10979.1, SRR1803616.233759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGGCT CTGGGCGAAA AGACTTCGAT GTGAAGCACA TTCTGCGACT CCGCTGGAAA 
CTCTTCAGCC ACCCGTCTCC TTCCACCGGC GGCCCGGCGG GGGGAGGCTG CCTGCAACAG 
GACGGCAGTG GCAGCTTTGA GCACTGGGGA CCCAGCCAGA GTCGCCTGCT CAAAAGCCAA 
GAGAGAAGCG GTGTGAGCAC TTTCTGGAAG AAGCCTTCCT CCTCTTCCTC TTCATCGTCC 
TCCCCGTCCT CTTCCTCCTC TTCCTTCAAT CCGCTGAATG GCACCCTGCT TCCAGTTGCC 
ACGAGGCTGC AGCAAGGGGC TCCTGGGCAG GGCACTCAGC AGCCAGCCAG GACTCTCTTC 
TACGTGGAGT CACTAGAGGA GGAGGTGGTG CCAGGCATGG ACTTTCCTGG ACCACACGAA 
AAAGGGCTGG TTCTGCAAGA GCTCAAAGTG GAGCCAGACA ACTCTAGCCA GGCAACAGGT 
GAAGGATGTG GACACAGGCT TGTTCTGAGT TCAGTCTCCG ACTATTTTGC GGCCATGTTT 
ACAAGTGATG TTTGTGAAGC CAAGCAAGAG GAGATCAAAA TGGAAGGCAT AGACCCCAAT 
GCTCTCTGGG ACCTTGTCCA ATTTGCATAT ACAGGCTGCT TGGAATTAAA AGAGGACACC 
ATTGAGAACC TTCTTGCTGC AGCGTGCCTT CTTCAGCTTC CACAGGTGGT GGAAGTGTGC 
TGCCACTTCC TCATGAAGCT TTTGCATCCA TCTAACTGTT TAGGAATTCG AGCCTTCGCA 
GATGCTCAAG GATGCATTGA GTTAATGAAG GTGGCCCACA GCTACACAAT GGAAAACATA 
ATGGAAGTTA TCAGAAATCA AGAGTTTTTA CTCCTTCCAG CTGAGGAGCT CCATAAACTA 
CTGGCCAGTG ATGATGTCAA TGTTCCTGAT GAAGAAACCA TCTTCCATGC ATTGATGATG 
TGGGTCAAGT ATGACATGCA GAGTAGATGC AATGACCTGA GCATGCTTCT TGCCTTTATA 
AGACTGCCAC TGCTTCCACC ACAGATATTG GCTGACCTAG AAAATCATGC ATTATTTAAG 
AATGATCTGG AATGTCAAAA GCTGATTCTA GAAGCAATGA AATACCATCT ATTGCCAGAA 
AGAAGAACTT TAATGCAAAG TCCGAGAACT AAACCCAGAA AATCTACAGT CGGAACTTTG 
TATGCTGTAG GAGGAATGGA TAACAACAAA GGAGCTACAA CTATAGAGAA ATATGATCTG 
AGAACAAATC TGTGGATCCA GGCAGGGATG ATGAATGGCA GAAGGCTGCA GTTTGGTGTG 
GCTGTTATTG ATGACAAACT CTTTGTAATT GGAGGTCGAG ATGGCTTAAA GACATTGAAC 
ACTGTTGAAT GTTACAATCC CAAAACCAAG ACATGGACTG TCTTACCACC AATGTCAACA 
CACAGACATG GTCTAGGTGT AACAGTACTT GAAGGCCCTA TTTATGCTGT GGGAGGCCAT 
GATGGCTGGA GCTATCTGAA TACAGTGGAA AGGTGGGATC CACAGAGTCA ACAATGGACA 
TTTGTAGCCA GTATGTCAAT TGCTCGGAGC ACAGTTGGTG TAGCAGCATT GAATGGCAAG 
TTGTATTCAG TTGGAGGTCG TGATGGAAGT TCCTGTTTGA GTTCAATGGA ATATTATGAT 
CCTCATACAA ATAAGTGGAA CATGTGTGCT CCCATGTGTA AGAGGAGAGG GGGTGTCGGA 
GTGGCCACAT GTGACGGTTT TCTTTATGCA GTAGGAGGTC ATGATGCTCC TGCTTCAAAT 
CACTGTTCCC GGCTACTGGA TTATGTAGAA AGATATGATC CCAAAACAGA CACTTGGACC 
ATGGTGGCTC CTTTGAGTAT GCCCAGAGAT GCTGTTGGGG TCTGTCTCCT TGGTGACAGA 
TTATATGCTG TTGGTGGCTA TGATGGACAG ACATACCTCA ACACTATGGA ATCCTATGAC 
CCACAAACTA ATGAGTGGAC ACAGATGGCT TCCTTGAATA TTGGGAGAGC AGGTGCCTGT 
GTGGTAGTCA TCAAGCAACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273654.1
CDS	795..2858
Translation	MSGSGRKDFDVKHILRLRWKLFSHPSPSTGGPAGGGCLQQDGSGSFEHWGPSQSRLLKSQERSGVSTFWKKPSSSSSSSSSPSSSSSSFNPLNGTLLPVATRLQQGAPGQGTQQPARTLFYVESLEEEVVPGMDFPGPHEKGLVLQELKVEPDNSSQATGEGCGHRLVLSSVSDYFAAMFTSDVCEAKQEEIKMEGIDPNALWDLVQFAYTGCLELKEDTIENLLAAACLLQLPQVVEVCCHFLMKLLHPSNCLGIRAFADAQGCIELMKVAHSYTMENIMEVIRNQEFLLLPAEELHKLLASDDVNVPDEETIFHALMMWVKYDMQSRCNDLSMLLAFIRLPLLPPQILADLENHALFKNDLECQKLILEAMKYHLLPERRTLMQSPRTKPRKSTVGTLYAVGGMDNNKGATTIEKYDLRTNLWIQAGMMNGRRLQFGVAVIDDKLFVIGGRDGLKTLNTVECYNPKTKTWTVLPPMSTHRHGLGVTVLEGPIYAVGGHDGWSYLNTVERWDPQSQQWTFVASMSIARSTVGVAALNGKLYSVGGRDGSSCLSSMEYYDPHTNKWNMCAPMCKRRGGVGVATCDGFLYAVGGHDAPASNHCSRLLDYVERYDPKTDTWTMVAPLSMPRDAVGVCLLGDRLYAVGGYDGQTYLNTMESYDPQTNEWTQMASLNIGRAGACVVVIKQP

Target ORF information:

RefSeq Version	NM_001286725.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kelch like family member 1 (KLHL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286725.1

ORF Insert Sequence:

ATGTCAGGCT CTGGGCGAAA AGACTTCGAT GTGAAGCACA TTCTGCGACT CCGCTGGAAA 
CTCTTCAGCC ACCCGTCTCC TTCCACCGGC GGCCCGGCGG GGGGAGGCTG CCTGCAACAG 
GACGGCAGTG GCAGCTTTGA GCACTGGGGA CCCAGCCAGA GTCGCCTGCT CAAAAGCCAA 
GAGAGAAGCG GTGTGAGCAC TTTCTGGAAG AAGCCTTCCT CCTCTTCCTC TTCATCGTCC 
TCCCCGTCCT CTTCCTCCTC TTCCTTCAAT CCGCTGAATG GCACCCTGCT TCCAGTTGCC 
ACGAGGCTGC AGCAAGGGGC TCCTGGGCAG GGCACTCAGC AGCCAGCCAG GACTCTCTTC 
TACGTGGAGT CACTAGAGGA GGAGGTGGTG CCAGGCATGG ACTTTCCTGG ACCACACGAA 
AAAGGGCTGG TTCTGCAAGA GCTCAAAGTG GAGCCAGACA ACTCTAGCCA GGCAACAGGT 
GAAGGATGTG GACACAGGCT TGTTCTGAGT TCAGTCTCCG ACTATTTTGC GGCCATGTTT 
ACAAGTGATG TTTGTGAAGC CAAGCAAGAG GAGATCAAAA TGGAAGGCAT AGACCCCAAT 
GCTCTCTGGG ACCTTGTCCA ATTTGCATAT ACAGGCTGCT TGGAATTAAA AGAGGACACC 
ATTGAGAACC TTCTTGCTGC AGCGTGCCTT CTTCAGCTTC CACAGGTGGT GGAAGTGTGC 
TGCCACTTCC TCATGAAGCT TTTGCATCCA TCTAACTGTT TAGGAATTCG AGCCTTCGCA 
GATGCTCAAG GATGCATTGA GTTAATGAAG GTGGCCCACA GCTACACAAT GGAAAACATA 
ATGGAAGTTA TCAGAAATCA AGAGTTTTTA CTCCTTCCAG CTGAGGAGCT CCATAAACTA 
CTGGCCAGTG ATGATGTCAA TGTTCCTGAT GAAGAAACCA TCTTCCATGC ATTGATGATG 
TGGGTCAAGT ATGACATGCA GAGTAGATGC AATGACCTGA GCATGCTTCT TGCCTTTATA 
AGACTGCCAC TGCTTCCACC ACAGATATTG GCTGACCTAG AAAATCATGC ATTATTTAAG 
AATGATCTGG AATGTCAAAA GCTGATTCTA GAAGCAATGA AATACCATCT ATTGCCAGAA 
AGAAGAACTT TAATGCAAAG TCCGAGAACT AAACCCAGAA AATCTACAGT CGGAACTTTG 
TATGCTGTAG GAGGAATGGA TAACAACAAA GGAGCTACAA CTATAGAGAA ATATGATCTG 
AGAACAAATC TGTGGATCCA GGCAGGGATG ATGAATGGCA GAAGGCTGCA GTTTGGTGTG 
GCTGTTATTG ATGACAAACT CTTTGTAATT GGAGGTCGAG ATGGCTTAAA GACATTGAAC 
ACTGTTGAAT GTTACAATCC CAAAACCAAG ACATGGACTG TCTTACCACC AATGTCAACA 
CACAGACATG GTCTAGGTGT AACAGTACTT GAAGGCCCTA TTTATGCTGT GGGAGGCCAT 
GATGGCTGGA GCTATCTGAA TACAGTGGAA AGGTGGGATC CACAGAGTCA ACAATGGACA 
TTTGTAGCCA GTATGTCAAT TGCTCGGAGC ACAGTTGGTG TAGCAGCATT GAATGGCAAG 
TTGTATTCAG TTGGAGGTCG TGATGGAAGT TCCTGTTTGA GTTCAATGGA ATATTATGAT 
CCTCATACAA ATAAGTGGAA CATGTGTGCT CCCATGTGTA AGAGGAGAGG GGGTGTCGGA 
GTGGCCACAT GTGACGGTTT TCTTTATGCA GTAGGAGGTC ATGATGCTCC TGCTTCAAAT 
CACTGTTCCC GGCTACTGGA TTATGTAGAA AGATATGATC CCAAAACAGA CACTTGGACC 
ATGGTGGCTC CTTTGAGTAT GCCCAGAGAT GCTGTTGGGG TCTGTCTCCT TGGTGACAGA 
TTATATGCTG TTGGTGGCTA TGATGGACAG ACATACCTCA ACACTATGGA ATCCTATGAC 
CCACAAACTA ATGAGTGGAC ACAGATGGCT TCCTTGAATA TTGGGAGAGC AGGTGCCTGT 
GTGGTAGTCA TCAAGCAACC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KLHL1 ( XM_017020678.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876167.1 Homo sapiens kelch like family member 1 (KLHL1), transcript variant X1, mRNA.

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CloneID	OHu84597
Clone ID Related Accession (Same CDS sequence)	XM_017020678.1 , XM_017020678.2
Accession Version	XM_017020678.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	kelch-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACCTC AAAGTGATTT GGACTCCAGT AGCTCTGAAG AATTCTATCA AGCTGTTCAT 
CATGCTGAGC AAACCTTCAG AAAGATGGAA AGTTATTTGA AGCAGCAGCA ACTTTGTGAT 
GTTATCCTGA TTGTTGGGAA CCGAAAGATA CCTGCACATA GGCTTGTTCT GAGTTCAGTC 
TCCGACTATT TTGCGGCCAT GTTTACAAGT GATGTTTGTG AAGCCAAGCA AGAGGAGATC 
AAAATGGAAG GCATAGACCC CAATGCTCTC TGGGACCTTG TCCAATTTGC ATATACAGGC 
TGCTTGGAAT TAAAAGAGGA CACCATTGAG AACCTTCTTG CTGCAGCGTG CCTTCTTCAG 
CTTCCACAGG TGGTGGAAGT GTGCTGCCAC TTCCTCATGA AGCTTTTGCA TCCATCTAAC 
TGTTTAGGAA TTCGAGCCTT CGCAGATGCT CAAGGATGCA TTGAGTTAAT GAAGGTGGCC 
CACAGCTACA CAATGGAAAA CATAATGGAA GTTATCAGAA ATCAAGAGTT TTTACTCCTT 
CCAGCTGAGG AGCTCCATAA ACTACTGGCC AGTGATGATG TCAATGTTCC TGATGAAGAA 
ACCATCTTCC ATGCATTGAT GATGTGGGTC AAGTATGACA TGCAGAGTAG ATGCAATGAC 
CTGAGCATGC TTCTTGCCTT TATAAGACTG CCACTGCTTC CACCACAGAT ATTGGCTGAC 
CTAGAAAATC ATGCATTATT TAAGAATGAT CTGGAATGTC AAAAGCTGAT TCTAGAAGCA 
ATGAAATACC ATCTATTGCC AGAAAGAAGA ACTTTAATGC AAAGTCCGAG AACTAAACCC 
AGAAAATCTA CAGTCGGAAC TTTGTATGCT GTAGGAGGAA TGGATAACAA CAAAGGAGCT 
ACAACTATAG AGAAATATGA TCTGAGAACA AATCTGTGGA TCCAGGCAGG GATGATGAAT 
GGCAGAAGGC TGCAGTTTGG TGTGGCTGTT ATTGATGACA AACTCTTTGT AATTGGAGGT 
CGAGATGGCT TAAAGACATT GAACACTGTT GAATGTTACA ATCCCAAAAC CAAGACATGG 
ACTGTCTTAC CACCAATGTC AACACACAGA CATGGTCTAG GTGTAACAGT ACTTGAAGGC 
CCTATTTATG CTGTGGGAGG CCATGATGGC TGGAGCTATC TGAATACAGT GGAAAGGTGG 
GATCCACAGA GTCAACAATG GACATTTGTA GCCAGTATGT CAATTGCTCG GAGCACAGTT 
GGTGTAGCAG CATTGAATGG CAAGTTGTAT TCAGTTGGAG GTCGTGATGG AAGTTCCTGT 
TTGAGTTCAA TGGAATATTA TGATCCTCAT ACAAATAAGT GGAACATGTG TGCTCCCATG 
TGTAAGAGGA GAGGGGGTGT CGGAGTGGCC ACATGTGACG GTTTTCTTTA TGCAGTAGGA 
GGTCATGATG CTCCTGCTTC AAATCACTGT TCCCGGCTAC TGGATTATGT AGAAAGATAT 
GATCCCAAAA CAGACACTTG GACCATGGTG GCTCCTTTGA GTATGCCCAG AGATGCTGTT 
GGGGTCTGTC TCCTTGGTGA CAGATTATAT GCTGTTGGTG GCTATGATGG ACAGACATAC 
CTCAACACTA TGGAATCCTA TGACCCACAA ACTAATGAGT GGACACAGAT GGCTTCCTTG 
AATATTGGGA GAGCAGGTGC CTGTGTGGTA GTCATCAAGC AACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876167.1
CDS	234..1961
Translation	MTPQSDLDSSSSEEFYQAVHHAEQTFRKMESYLKQQQLCDVILIVGNRKIPAHRLVLSSVSDYFAAMFTSDVCEAKQEEIKMEGIDPNALWDLVQFAYTGCLELKEDTIENLLAAACLLQLPQVVEVCCHFLMKLLHPSNCLGIRAFADAQGCIELMKVAHSYTMENIMEVIRNQEFLLLPAEELHKLLASDDVNVPDEETIFHALMMWVKYDMQSRCNDLSMLLAFIRLPLLPPQILADLENHALFKNDLECQKLILEAMKYHLLPERRTLMQSPRTKPRKSTVGTLYAVGGMDNNKGATTIEKYDLRTNLWIQAGMMNGRRLQFGVAVIDDKLFVIGGRDGLKTLNTVECYNPKTKTWTVLPPMSTHRHGLGVTVLEGPIYAVGGHDGWSYLNTVERWDPQSQQWTFVASMSIARSTVGVAALNGKLYSVGGRDGSSCLSSMEYYDPHTNKWNMCAPMCKRRGGVGVATCDGFLYAVGGHDAPASNHCSRLLDYVERYDPKTDTWTMVAPLSMPRDAVGVCLLGDRLYAVGGYDGQTYLNTMESYDPQTNEWTQMASLNIGRAGACVVVIKQP

Target ORF information:

RefSeq Version	XM_017020678.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kelch like family member 1 (KLHL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020678.1

ORF Insert Sequence:

ATGACACCTC AAAGTGATTT GGACTCCAGT AGCTCTGAAG AATTCTATCA AGCTGTTCAT 
CATGCTGAGC AAACCTTCAG AAAGATGGAA AGTTATTTGA AGCAGCAGCA ACTTTGTGAT 
GTTATCCTGA TTGTTGGGAA CCGAAAGATA CCTGCACATA GGCTTGTTCT GAGTTCAGTC 
TCCGACTATT TTGCGGCCAT GTTTACAAGT GATGTTTGTG AAGCCAAGCA AGAGGAGATC 
AAAATGGAAG GCATAGACCC CAATGCTCTC TGGGACCTTG TCCAATTTGC ATATACAGGC 
TGCTTGGAAT TAAAAGAGGA CACCATTGAG AACCTTCTTG CTGCAGCGTG CCTTCTTCAG 
CTTCCACAGG TGGTGGAAGT GTGCTGCCAC TTCCTCATGA AGCTTTTGCA TCCATCTAAC 
TGTTTAGGAA TTCGAGCCTT CGCAGATGCT CAAGGATGCA TTGAGTTAAT GAAGGTGGCC 
CACAGCTACA CAATGGAAAA CATAATGGAA GTTATCAGAA ATCAAGAGTT TTTACTCCTT 
CCAGCTGAGG AGCTCCATAA ACTACTGGCC AGTGATGATG TCAATGTTCC TGATGAAGAA 
ACCATCTTCC ATGCATTGAT GATGTGGGTC AAGTATGACA TGCAGAGTAG ATGCAATGAC 
CTGAGCATGC TTCTTGCCTT TATAAGACTG CCACTGCTTC CACCACAGAT ATTGGCTGAC 
CTAGAAAATC ATGCATTATT TAAGAATGAT CTGGAATGTC AAAAGCTGAT TCTAGAAGCA 
ATGAAATACC ATCTATTGCC AGAAAGAAGA ACTTTAATGC AAAGTCCGAG AACTAAACCC 
AGAAAATCTA CAGTCGGAAC TTTGTATGCT GTAGGAGGAA TGGATAACAA CAAAGGAGCT 
ACAACTATAG AGAAATATGA TCTGAGAACA AATCTGTGGA TCCAGGCAGG GATGATGAAT 
GGCAGAAGGC TGCAGTTTGG TGTGGCTGTT ATTGATGACA AACTCTTTGT AATTGGAGGT 
CGAGATGGCT TAAAGACATT GAACACTGTT GAATGTTACA ATCCCAAAAC CAAGACATGG 
ACTGTCTTAC CACCAATGTC AACACACAGA CATGGTCTAG GTGTAACAGT ACTTGAAGGC 
CCTATTTATG CTGTGGGAGG CCATGATGGC TGGAGCTATC TGAATACAGT GGAAAGGTGG 
GATCCACAGA GTCAACAATG GACATTTGTA GCCAGTATGT CAATTGCTCG GAGCACAGTT 
GGTGTAGCAG CATTGAATGG CAAGTTGTAT TCAGTTGGAG GTCGTGATGG AAGTTCCTGT 
TTGAGTTCAA TGGAATATTA TGATCCTCAT ACAAATAAGT GGAACATGTG TGCTCCCATG 
TGTAAGAGGA GAGGGGGTGT CGGAGTGGCC ACATGTGACG GTTTTCTTTA TGCAGTAGGA 
GGTCATGATG CTCCTGCTTC AAATCACTGT TCCCGGCTAC TGGATTATGT AGAAAGATAT 
GATCCCAAAA CAGACACTTG GACCATGGTG GCTCCTTTGA GTATGCCCAG AGATGCTGTT 
GGGGTCTGTC TCCTTGGTGA CAGATTATAT GCTGTTGGTG GCTATGATGG ACAGACATAC 
CTCAACACTA TGGAATCCTA TGACCCACAA ACTAATGAGT GGACACAGAT GGCTTCCTTG 
AATATTGGGA GAGCAGGTGC CTGTGTGGTA GTCATCAAGC AACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KLHL1 ( XM_017020678.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876167.1 Homo sapiens kelch like family member 1 (KLHL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu84597
Clone ID Related Accession (Same CDS sequence)	XM_017020678.1 , XM_017020678.2
Accession Version	XM_017020678.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kelch-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACCTC AAAGTGATTT GGACTCCAGT AGCTCTGAAG AATTCTATCA AGCTGTTCAT 
CATGCTGAGC AAACCTTCAG AAAGATGGAA AGTTATTTGA AGCAGCAGCA ACTTTGTGAT 
GTTATCCTGA TTGTTGGGAA CCGAAAGATA CCTGCACATA GGCTTGTTCT GAGTTCAGTC 
TCCGACTATT TTGCGGCCAT GTTTACAAGT GATGTTTGTG AAGCCAAGCA AGAGGAGATC 
AAAATGGAAG GCATAGACCC CAATGCTCTC TGGGACCTTG TCCAATTTGC ATATACAGGC 
TGCTTGGAAT TAAAAGAGGA CACCATTGAG AACCTTCTTG CTGCAGCGTG CCTTCTTCAG 
CTTCCACAGG TGGTGGAAGT GTGCTGCCAC TTCCTCATGA AGCTTTTGCA TCCATCTAAC 
TGTTTAGGAA TTCGAGCCTT CGCAGATGCT CAAGGATGCA TTGAGTTAAT GAAGGTGGCC 
CACAGCTACA CAATGGAAAA CATAATGGAA GTTATCAGAA ATCAAGAGTT TTTACTCCTT 
CCAGCTGAGG AGCTCCATAA ACTACTGGCC AGTGATGATG TCAATGTTCC TGATGAAGAA 
ACCATCTTCC ATGCATTGAT GATGTGGGTC AAGTATGACA TGCAGAGTAG ATGCAATGAC 
CTGAGCATGC TTCTTGCCTT TATAAGACTG CCACTGCTTC CACCACAGAT ATTGGCTGAC 
CTAGAAAATC ATGCATTATT TAAGAATGAT CTGGAATGTC AAAAGCTGAT TCTAGAAGCA 
ATGAAATACC ATCTATTGCC AGAAAGAAGA ACTTTAATGC AAAGTCCGAG AACTAAACCC 
AGAAAATCTA CAGTCGGAAC TTTGTATGCT GTAGGAGGAA TGGATAACAA CAAAGGAGCT 
ACAACTATAG AGAAATATGA TCTGAGAACA AATCTGTGGA TCCAGGCAGG GATGATGAAT 
GGCAGAAGGC TGCAGTTTGG TGTGGCTGTT ATTGATGACA AACTCTTTGT AATTGGAGGT 
CGAGATGGCT TAAAGACATT GAACACTGTT GAATGTTACA ATCCCAAAAC CAAGACATGG 
ACTGTCTTAC CACCAATGTC AACACACAGA CATGGTCTAG GTGTAACAGT ACTTGAAGGC 
CCTATTTATG CTGTGGGAGG CCATGATGGC TGGAGCTATC TGAATACAGT GGAAAGGTGG 
GATCCACAGA GTCAACAATG GACATTTGTA GCCAGTATGT CAATTGCTCG GAGCACAGTT 
GGTGTAGCAG CATTGAATGG CAAGTTGTAT TCAGTTGGAG GTCGTGATGG AAGTTCCTGT 
TTGAGTTCAA TGGAATATTA TGATCCTCAT ACAAATAAGT GGAACATGTG TGCTCCCATG 
TGTAAGAGGA GAGGGGGTGT CGGAGTGGCC ACATGTGACG GTTTTCTTTA TGCAGTAGGA 
GGTCATGATG CTCCTGCTTC AAATCACTGT TCCCGGCTAC TGGATTATGT AGAAAGATAT 
GATCCCAAAA CAGACACTTG GACCATGGTG GCTCCTTTGA GTATGCCCAG AGATGCTGTT 
GGGGTCTGTC TCCTTGGTGA CAGATTATAT GCTGTTGGTG GCTATGATGG ACAGACATAC 
CTCAACACTA TGGAATCCTA TGACCCACAA ACTAATGAGT GGACACAGAT GGCTTCCTTG 
AATATTGGGA GAGCAGGTGC CTGTGTGGTA GTCATCAAGC AACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876167.1
CDS	235..1962
Translation	MTPQSDLDSSSSEEFYQAVHHAEQTFRKMESYLKQQQLCDVILIVGNRKIPAHRLVLSSVSDYFAAMFTSDVCEAKQEEIKMEGIDPNALWDLVQFAYTGCLELKEDTIENLLAAACLLQLPQVVEVCCHFLMKLLHPSNCLGIRAFADAQGCIELMKVAHSYTMENIMEVIRNQEFLLLPAEELHKLLASDDVNVPDEETIFHALMMWVKYDMQSRCNDLSMLLAFIRLPLLPPQILADLENHALFKNDLECQKLILEAMKYHLLPERRTLMQSPRTKPRKSTVGTLYAVGGMDNNKGATTIEKYDLRTNLWIQAGMMNGRRLQFGVAVIDDKLFVIGGRDGLKTLNTVECYNPKTKTWTVLPPMSTHRHGLGVTVLEGPIYAVGGHDGWSYLNTVERWDPQSQQWTFVASMSIARSTVGVAALNGKLYSVGGRDGSSCLSSMEYYDPHTNKWNMCAPMCKRRGGVGVATCDGFLYAVGGHDAPASNHCSRLLDYVERYDPKTDTWTMVAPLSMPRDAVGVCLLGDRLYAVGGYDGQTYLNTMESYDPQTNEWTQMASLNIGRAGACVVVIKQP

Target ORF information:

RefSeq Version	XM_017020678.2
Organism	Homo sapiens(human)
Definition	Homo sapiens kelch like family member 1 (KLHL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020678.2

ORF Insert Sequence:

ATGACACCTC AAAGTGATTT GGACTCCAGT AGCTCTGAAG AATTCTATCA AGCTGTTCAT 
CATGCTGAGC AAACCTTCAG AAAGATGGAA AGTTATTTGA AGCAGCAGCA ACTTTGTGAT 
GTTATCCTGA TTGTTGGGAA CCGAAAGATA CCTGCACATA GGCTTGTTCT GAGTTCAGTC 
TCCGACTATT TTGCGGCCAT GTTTACAAGT GATGTTTGTG AAGCCAAGCA AGAGGAGATC 
AAAATGGAAG GCATAGACCC CAATGCTCTC TGGGACCTTG TCCAATTTGC ATATACAGGC 
TGCTTGGAAT TAAAAGAGGA CACCATTGAG AACCTTCTTG CTGCAGCGTG CCTTCTTCAG 
CTTCCACAGG TGGTGGAAGT GTGCTGCCAC TTCCTCATGA AGCTTTTGCA TCCATCTAAC 
TGTTTAGGAA TTCGAGCCTT CGCAGATGCT CAAGGATGCA TTGAGTTAAT GAAGGTGGCC 
CACAGCTACA CAATGGAAAA CATAATGGAA GTTATCAGAA ATCAAGAGTT TTTACTCCTT 
CCAGCTGAGG AGCTCCATAA ACTACTGGCC AGTGATGATG TCAATGTTCC TGATGAAGAA 
ACCATCTTCC ATGCATTGAT GATGTGGGTC AAGTATGACA TGCAGAGTAG ATGCAATGAC 
CTGAGCATGC TTCTTGCCTT TATAAGACTG CCACTGCTTC CACCACAGAT ATTGGCTGAC 
CTAGAAAATC ATGCATTATT TAAGAATGAT CTGGAATGTC AAAAGCTGAT TCTAGAAGCA 
ATGAAATACC ATCTATTGCC AGAAAGAAGA ACTTTAATGC AAAGTCCGAG AACTAAACCC 
AGAAAATCTA CAGTCGGAAC TTTGTATGCT GTAGGAGGAA TGGATAACAA CAAAGGAGCT 
ACAACTATAG AGAAATATGA TCTGAGAACA AATCTGTGGA TCCAGGCAGG GATGATGAAT 
GGCAGAAGGC TGCAGTTTGG TGTGGCTGTT ATTGATGACA AACTCTTTGT AATTGGAGGT 
CGAGATGGCT TAAAGACATT GAACACTGTT GAATGTTACA ATCCCAAAAC CAAGACATGG 
ACTGTCTTAC CACCAATGTC AACACACAGA CATGGTCTAG GTGTAACAGT ACTTGAAGGC 
CCTATTTATG CTGTGGGAGG CCATGATGGC TGGAGCTATC TGAATACAGT GGAAAGGTGG 
GATCCACAGA GTCAACAATG GACATTTGTA GCCAGTATGT CAATTGCTCG GAGCACAGTT 
GGTGTAGCAG CATTGAATGG CAAGTTGTAT TCAGTTGGAG GTCGTGATGG AAGTTCCTGT 
TTGAGTTCAA TGGAATATTA TGATCCTCAT ACAAATAAGT GGAACATGTG TGCTCCCATG 
TGTAAGAGGA GAGGGGGTGT CGGAGTGGCC ACATGTGACG GTTTTCTTTA TGCAGTAGGA 
GGTCATGATG CTCCTGCTTC AAATCACTGT TCCCGGCTAC TGGATTATGT AGAAAGATAT 
GATCCCAAAA CAGACACTTG GACCATGGTG GCTCCTTTGA GTATGCCCAG AGATGCTGTT 
GGGGTCTGTC TCCTTGGTGA CAGATTATAT GCTGTTGGTG GCTATGATGG ACAGACATAC 
CTCAACACTA TGGAATCCTA TGACCCACAA ACTAATGAGT GGACACAGAT GGCTTCCTTG 
AATATTGGGA GAGCAGGTGC CTGTGTGGTA GTCATCAAGC AACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KLHL1 ( XM_017020679.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876168.1 Homo sapiens kelch like family member 1 (KLHL1), transcript variant X2, mRNA.

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CloneID	OHu84598
Clone ID Related Accession (Same CDS sequence)	XM_017020679.1
Accession Version	XM_017020679.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kelch-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCAGA GGCTTGTTCT GAGTTCAGTC TCCGACTATT TTGCGGCCAT GTTTACAAGT 
GATGTTTGTG AAGCCAAGCA AGAGGAGATC AAAATGGAAG GCATAGACCC CAATGCTCTC 
TGGGACCTTG TCCAATTTGC ATATACAGGC TGCTTGGAAT TAAAAGAGGA CACCATTGAG 
AACCTTCTTG CTGCAGCGTG CCTTCTTCAG CTTCCACAGG TGGTGGAAGT GTGCTGCCAC 
TTCCTCATGA AGCTTTTGCA TCCATCTAAC TGTTTAGGAA TTCGAGCCTT CGCAGATGCT 
CAAGGATGCA TTGAGTTAAT GAAGGTGGCC CACAGCTACA CAATGGAAAA CATAATGGAA 
GTTATCAGAA ATCAAGAGTT TTTACTCCTT CCAGCTGAGG AGCTCCATAA ACTACTGGCC 
AGTGATGATG TCAATGTTCC TGATGAAGAA ACCATCTTCC ATGCATTGAT GATGTGGGTC 
AAGTATGACA TGCAGAGTAG ATGCAATGAC CTGAGCATGC TTCTTGCCTT TATAAGACTG 
CCACTGCTTC CACCACAGAT ATTGGCTGAC CTAGAAAATC ATGCATTATT TAAGAATGAT 
CTGGAATGTC AAAAGCTGAT TCTAGAAGCA ATGAAATACC ATCTATTGCC AGAAAGAAGA 
ACTTTAATGC AAAGTCCGAG AACTAAACCC AGAAAATCTA CAGTCGGAAC TTTGTATGCT 
GTAGGAGGAA TGGATAACAA CAAAGGAGCT ACAACTATAG AGAAATATGA TCTGAGAACA 
AATCTGTGGA TCCAGGCAGG GATGATGAAT GGCAGAAGGC TGCAGTTTGG TGTGGCTGTT 
ATTGATGACA AACTCTTTGT AATTGGAGGT CGAGATGGCT TAAAGACATT GAACACTGTT 
GAATGTTACA ATCCCAAAAC CAAGACATGG ACTGTCTTAC CACCAATGTC AACACACAGA 
CATGGTCTAG GTGTAACAGT ACTTGAAGGC CCTATTTATG CTGTGGGAGG CCATGATGGC 
TGGAGCTATC TGAATACAGT GGAAAGGTGG GATCCACAGA GTCAACAATG GACATTTGTA 
GCCAGTATGT CAATTGCTCG GAGCACAGTT GGTGTAGCAG CATTGAATGG CAAGTTGTAT 
TCAGTTGGAG GTCGTGATGG AAGTTCCTGT TTGAGTTCAA TGGAATATTA TGATCCTCAT 
ACAAATAAGT GGAACATGTG TGCTCCCATG TGTAAGAGGA GAGGGGGTGT CGGAGTGGCC 
ACATGTGACG GTTTTCTTTA TGCAGTAGGA GGTCATGATG CTCCTGCTTC AAATCACTGT 
TCCCGGCTAC TGGATTATGT AGAAAGATAT GATCCCAAAA CAGACACTTG GACCATGGTG 
GCTCCTTTGA GTATGCCCAG AGATGCTGTT GGGGTCTGTC TCCTTGGTGA CAGATTATAT 
GCTGTTGGTG GCTATGATGG ACAGACATAC CTCAACACTA TGGAATCCTA TGACCCACAA 
ACTAATGAGT GGACACAGAT GGCTTCCTTG AATATTGGGA GAGCAGGTGC CTGTGTGGTA 
GTCATCAAGC AACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876168.1
CDS	971..2548
Translation	MNQRLVLSSVSDYFAAMFTSDVCEAKQEEIKMEGIDPNALWDLVQFAYTGCLELKEDTIENLLAAACLLQLPQVVEVCCHFLMKLLHPSNCLGIRAFADAQGCIELMKVAHSYTMENIMEVIRNQEFLLLPAEELHKLLASDDVNVPDEETIFHALMMWVKYDMQSRCNDLSMLLAFIRLPLLPPQILADLENHALFKNDLECQKLILEAMKYHLLPERRTLMQSPRTKPRKSTVGTLYAVGGMDNNKGATTIEKYDLRTNLWIQAGMMNGRRLQFGVAVIDDKLFVIGGRDGLKTLNTVECYNPKTKTWTVLPPMSTHRHGLGVTVLEGPIYAVGGHDGWSYLNTVERWDPQSQQWTFVASMSIARSTVGVAALNGKLYSVGGRDGSSCLSSMEYYDPHTNKWNMCAPMCKRRGGVGVATCDGFLYAVGGHDAPASNHCSRLLDYVERYDPKTDTWTMVAPLSMPRDAVGVCLLGDRLYAVGGYDGQTYLNTMESYDPQTNEWTQMASLNIGRAGACVVVIKQP

Target ORF information:

RefSeq Version	XM_017020679.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kelch like family member 1 (KLHL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020679.1

ORF Insert Sequence:

ATGAATCAGA GGCTTGTTCT GAGTTCAGTC TCCGACTATT TTGCGGCCAT GTTTACAAGT 
GATGTTTGTG AAGCCAAGCA AGAGGAGATC AAAATGGAAG GCATAGACCC CAATGCTCTC 
TGGGACCTTG TCCAATTTGC ATATACAGGC TGCTTGGAAT TAAAAGAGGA CACCATTGAG 
AACCTTCTTG CTGCAGCGTG CCTTCTTCAG CTTCCACAGG TGGTGGAAGT GTGCTGCCAC 
TTCCTCATGA AGCTTTTGCA TCCATCTAAC TGTTTAGGAA TTCGAGCCTT CGCAGATGCT 
CAAGGATGCA TTGAGTTAAT GAAGGTGGCC CACAGCTACA CAATGGAAAA CATAATGGAA 
GTTATCAGAA ATCAAGAGTT TTTACTCCTT CCAGCTGAGG AGCTCCATAA ACTACTGGCC 
AGTGATGATG TCAATGTTCC TGATGAAGAA ACCATCTTCC ATGCATTGAT GATGTGGGTC 
AAGTATGACA TGCAGAGTAG ATGCAATGAC CTGAGCATGC TTCTTGCCTT TATAAGACTG 
CCACTGCTTC CACCACAGAT ATTGGCTGAC CTAGAAAATC ATGCATTATT TAAGAATGAT 
CTGGAATGTC AAAAGCTGAT TCTAGAAGCA ATGAAATACC ATCTATTGCC AGAAAGAAGA 
ACTTTAATGC AAAGTCCGAG AACTAAACCC AGAAAATCTA CAGTCGGAAC TTTGTATGCT 
GTAGGAGGAA TGGATAACAA CAAAGGAGCT ACAACTATAG AGAAATATGA TCTGAGAACA 
AATCTGTGGA TCCAGGCAGG GATGATGAAT GGCAGAAGGC TGCAGTTTGG TGTGGCTGTT 
ATTGATGACA AACTCTTTGT AATTGGAGGT CGAGATGGCT TAAAGACATT GAACACTGTT 
GAATGTTACA ATCCCAAAAC CAAGACATGG ACTGTCTTAC CACCAATGTC AACACACAGA 
CATGGTCTAG GTGTAACAGT ACTTGAAGGC CCTATTTATG CTGTGGGAGG CCATGATGGC 
TGGAGCTATC TGAATACAGT GGAAAGGTGG GATCCACAGA GTCAACAATG GACATTTGTA 
GCCAGTATGT CAATTGCTCG GAGCACAGTT GGTGTAGCAG CATTGAATGG CAAGTTGTAT 
TCAGTTGGAG GTCGTGATGG AAGTTCCTGT TTGAGTTCAA TGGAATATTA TGATCCTCAT 
ACAAATAAGT GGAACATGTG TGCTCCCATG TGTAAGAGGA GAGGGGGTGT CGGAGTGGCC 
ACATGTGACG GTTTTCTTTA TGCAGTAGGA GGTCATGATG CTCCTGCTTC AAATCACTGT 
TCCCGGCTAC TGGATTATGT AGAAAGATAT GATCCCAAAA CAGACACTTG GACCATGGTG 
GCTCCTTTGA GTATGCCCAG AGATGCTGTT GGGGTCTGTC TCCTTGGTGA CAGATTATAT 
GCTGTTGGTG GCTATGATGG ACAGACATAC CTCAACACTA TGGAATCCTA TGACCCACAA 
ACTAATGAGT GGACACAGAT GGCTTCCTTG AATATTGGGA GAGCAGGTGC CTGTGTGGTA 
GTCATCAAGC AACCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Update on the Kelch-like (KLHL) gene family.
Human genomics713(2013 May)
Dhanoa BS,Cogliati T,Satish AG,Bruford EA,Friedman JS

Genome-wide contribution of genotype by environment interaction to variation of diabetes-related traits.
PloS one8(10)e77442(2013)
Zheng JS,Arnett DK,Lee YC,Shen J,Parnell LD,Smith CE,Richardson K,Li D,Borecki IB,Ordov?s JM,Lai CQ

Genome-wide association study of d-amphetamine response in healthy volunteers identifies putative associations, including cadherin 13 (CDH13).
PloS one7(8)e42646(2012)
Hart AB,Engelhardt BE,Wardle MC,Sokoloff G,Stephens M,de Wit H,Palmer AA

Influence of MRP2 on MPA pharmacokinetics in renal transplant recipients-results of the Pharmacogenomic Substudy within the Symphony Study.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association26(11)3784-93(2011 Nov)
Lloberas N,Torras J,Cruzado JM,Andreu F,Oppenheimer F,S?nchez-Plumed J,Gentil M?,Brunet M,Ekberg H,Griny? JM,

T-type current modulation by the actin-binding protein Kelch-like 1.
American journal of physiology. Cell physiology298(6)C1353-62(2010 Jun)
Aromolaran KA,Benzow KA,Cribbs LL,Koob MD,Piedras-Renter?a ES

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KLHL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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