PSME1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSME1
Entrez Gene ID	5720
Full Name	proteasome activator subunit 1
Synonyms	HEL-S-129m,IFI5111,PA28A,PA28alpha,REGalpha
General protein information	Preferred Names proteasome activator subunit 1 Names proteasome activator complex subunit 1 11S regulator complex subunit alpha 29-kD MCP activator subunit IGUP I-5111 activator of multicatalytic protease subunit 1 epididymis secretory sperm binding protein Li 129m interferon gamma up-regulated I-5111 protein interferon-gamma IEF SSP 5111 interferon-gamma-inducible protein 5111 proteasome (prosome, macropain) activator subunit 1 (PA28 alpha)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q12
Summary	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the alpha subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three alpha and three beta subunits combine to form a heterohexameric ring. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013].
Disorder MIM:	600654

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281529.1	NP_001268458.1	proteasome activator complex subunit 1 isoform 4
NM_001281529.2	NP_001268458.1	proteasome activator complex subunit 1 isoform 4
NM_006263.4	NP_006254.1	proteasome activator complex subunit 1 isoform 1
NM_001281528.2	NP_001268457.1	proteasome activator complex subunit 1 isoform 3
NM_006263.3	NP_006254.1	proteasome activator complex subunit 1 isoform 1
NM_001281528.1	NP_001268457.1	proteasome activator complex subunit 1 isoform 3
NM_176783.2	NP_788955.1	proteasome activator complex subunit 1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	PSME1	NP_006254.1
	PSME1	XP_001104073.1
Canis lupus familiaris (dog)	PSME1	XP_537380.1
Bos taurus (cattle)	PSME1	NP_001019680.2
Pan troglodytes (chimpanzee)	PSME1	XP_509864.3
Danio rerio (zebrafish)	psme1	NP_571450.1
Mus musculus (house mouse)	Psme1	NP_035319.1
Rattus norvegicus (Norway rat)	Psme1	NP_058960.2
Xenopus tropicalis (tropical clawed frog)	psme1	NP_001011302.2

Gene Ontology
Bibliography

Related articles in PubMed

Circadian modulation of proteasome activity and accumulation of oxidized protein in human embryonic kidney HEK 293 cells and primary dermal fibroblasts.
Desvergne A, Ugarte N, Radjei S, Gareil M, Petropoulos I, Friguet B
Free radical biology & medicine94195-207(2016 05)

Overexpression of proteasomal activator PA28? serves as a prognostic factor in oral squamous cell carcinoma.
Feng X, Jiang Y, Xie L, Jiang L, Li J, Sun C, Xu H, Wang R, Zhou M, Zhou Y, Dan H, Wang Z, Ji N, Deng P, Liao G, Geng N, Wang Y, Zhang D, Lin Y, Ye L, Liang X, Li L, Luo G, Feng M, Fang J, Zeng X, Wang Z, Chen Q
Journal of experimental & clinical cancer research : CR3535(2016 Feb)

The proteasome immunosubunits, PA28 and ER-aminopeptidase 1 protect melanoma cells from efficient MART-126-35 -specific T-cell recognition.
Keller M, Ebstein F, B?rger E, Textoris-Taube K, Gorny X, Urban S, Zhao F, Dannenberg T, Sucker A, Keller C, Saveanu L, Kr?ger E, Rothk?tter HJ, Dahlmann B, Henklein P, Voigt A, Kuckelkorn U, Paschen A, Kloetzel PM, Seifert U
European journal of immunology45(12)3257-68(2015 Dec)

Proteasome activator complex PA28 identified as an accessible target in prostate cancer by in vivo selection of human antibodies.
S?nchez-Mart?n D, Mart?nez-Torrecuadrada J, Teesalu T, Sugahara KN, Alvarez-Cienfuegos A, Xim?nez-Emb?n P, Fern?ndez-Peri??ez R, Mart?n MT, Molina-Privado I, Ruppen-Ca??s I, Blanco-Toribio A, Ca?amero M, Cuesta AM, Compte M, Kremer L, Bellas C, Alonso-Camino V, Guijarro-Mu?oz I, Sanz L, Ruoslahti E, Alvarez-Vallina L
Proceedings of the National Academy of Sciences of the United States of America110(34)13791-6(2013 Aug)

Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFN? in mouse pancreatic ?-cells.
Freudenburg W, Gautam M, Chakraborty P, James J, Richards J, Salvatori AS, Baldwin A, Schriewer J, Buller RM, Corbett JA, Skowyra D
PloS one8(2)e52408(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Such a modulation of proteasome activity is explained, at least in part, by the circadian expression of both Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) and the proteasome activator PA28ab
Title: Circadian modulation of proteasome activity and accumulation of oxidized protein in human embryonic kidney HEK 293 cells and primary dermal fibroblasts.

Results show that PA28alpha was found to be overexpressed in oral squamous cell carcinoma cell lines and tumor tissues. High expression of PA28alpha was significantly associated with recurrence and poorer overall survival.
Title: Overexpression of proteasomal activator PA28? serves as a prognostic factor in oral squamous cell carcinoma.

Constitutive expression of PA28 and ERAP1 in melanoma cells indicate that both interfere with MART-1(26-35) epitope generation even in the absence of IFN-gamma
Title: The proteasome immunosubunits, PA28 and ER-aminopeptidase 1 protect melanoma cells from efficient MART-126-35 -specific T-cell recognition.

PA28 is a tumor marker and a potential target for therapeutic intervention in prostate cancer.
Title: Proteasome activator complex PA28 identified as an accessible target in prostate cancer by in vivo selection of human antibodies.

Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFNbeta in mouse pancreatic beta-cells.
Title: Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFN? in mouse pancreatic ?-cells.

The following PSME1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSME1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09760	NM_176783.2 Latest version!	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09777	NM_001281528.2 Latest version!	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu10027	NM_006263.4 Latest version!	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09693	NM_001281529.2 Latest version!	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu09693	NM_001281529.1	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu10027	NM_006263.3	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09777	NM_001281528.1	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSME1 ( NM_176783.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_788955.1 Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09760
Clone ID Related Accession (Same CDS sequence)	NM_176783.2
Accession Version	NM_176783.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	proteasome activator complex subunit 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BJ993879.1, BQ060125.1, BX248293.1 and BM981616.1. On Jul 31, 2013 this sequence version replaced NM_176783.1. Transcript Variant: This variant (2) contains an alternate 3' structure, and thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (3) has a distinct and longer C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.337966.1, SRR1660807.136982.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG TGAGGAGGCA AGGGCAGGGC AGGGGTGGGC AGAGGCAGCT TTCCCAGGCC 
ACCCACTCCC TGACCCTGCA GGCTAGGGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_788955.1
CDS	106..858
Translation	MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYVRRQGQGRGGQRQLSQATHSLTLQARG

Target ORF information:

RefSeq Version	NM_176783.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176783.2

ORF Insert Sequence:

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG TGAGGAGGCA AGGGCAGGGC AGGGGTGGGC AGAGGCAGCT TTCCCAGGCC 
ACCCACTCCC TGACCCTGCA GGCTAGGGGT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSME1 ( NM_001281528.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268457.1 Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09777
Clone ID Related Accession (Same CDS sequence)	NM_001281528.2 , NM_001281528.1
Accession Version	NM_001281528.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	proteasome activator complex subunit 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BJ993879.1, BU192084.1 and BM981616.1. On May 31, 2019 this sequence version replaced NM_001281528.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 3, which has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.456110.1, SRR5189655.25938.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG TGAGGAGGCT GTGTTATATG ACATCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268457.1
CDS	70..771
Translation	MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYVRRLCYMTSS

Target ORF information:

RefSeq Version	NM_001281528.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281528.2

ORF Insert Sequence:

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG TGAGGAGGCT GTGTTATATG ACATCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSME1 ( NM_001281528.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268457.1 Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu09777
Clone ID Related Accession (Same CDS sequence)	NM_001281528.2 , NM_001281528.1
Accession Version	NM_001281528.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	proteasome activator complex subunit 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BJ993879.1, BU192084.1 and BM981616.1. Transcript Variant: This variant (3) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 3, which has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.182976.1, SRR1163657.456110.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG TGAGGAGGCT GTGTTATATG ACATCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268457.1
CDS	106..807
Translation	MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYVRRLCYMTSS

Target ORF information:

RefSeq Version	NM_001281528.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281528.1

ORF Insert Sequence:

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG TGAGGAGGCT GTGTTATATG ACATCATCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSME1 ( NM_006263.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006254.1 Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10027
Clone ID Related Accession (Same CDS sequence)	NM_006263.4 , NM_006263.3
Accession Version	NM_006263.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	proteasome activator complex subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L07633.1 and BM981616.1. On Nov 23, 2018 this sequence version replaced NM_006263.3. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.87432.1, SRR5189655.21876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000206451.11/ ENSP00000206451.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG CTGTGTTATA TGACATCATC CTGAAGAACT TCGAGAAGCT CAAGAAGCCC 
AGGGGAGAAA CAAAGGGAAT GATCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006254.1
CDS	70..819
Translation	MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

Target ORF information:

RefSeq Version	NM_006263.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006263.4

ORF Insert Sequence:

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG CTGTGTTATA TGACATCATC CTGAAGAACT TCGAGAAGCT CAAGAAGCCC 
AGGGGAGAAA CAAAGGGAAT GATCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSME1 ( NM_006263.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006254.1 Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 1, mRNA.

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CloneID	OHu10027
Clone ID Related Accession (Same CDS sequence)	NM_006263.4 , NM_006263.3
Accession Version	NM_006263.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	proteasome activator complex subunit 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BJ993879.1, L07633.1 and BM981616.1. On Jul 31, 2013 this sequence version replaced NM_006263.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.382506.1, SRR1163657.84374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG CTGTGTTATA TGACATCATC CTGAAGAACT TCGAGAAGCT CAAGAAGCCC 
AGGGGAGAAA CAAAGGGAAT GATCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006254.1
CDS	106..855
Translation	MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQEKVFELMTSLHTKLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

Target ORF information:

RefSeq Version	NM_006263.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006263.3

ORF Insert Sequence:

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGG AGAAGGTGTT TGAGCTGATG 
ACCAGCCTCC ACACCAAGCT AGAAGGCTTC CACACTCAAA TCTCTAAGTA TTTCTCTGAG 
CGTGGTGATG CAGTGACTAA AGCAGCCAAG CAGCCCCATG TGGGTGATTA TCGGCAGCTG 
GTGCACGAGC TGGATGAGGC AGAGTACCGG GACATCCGGC TGATGGTCAT GGAGATCCGC 
AATGCTTATG CTGTGTTATA TGACATCATC CTGAAGAACT TCGAGAAGCT CAAGAAGCCC 
AGGGGAGAAA CAAAGGGAAT GATCTATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSME1 ( NM_001281529.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268458.1 Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu09693
Clone ID Related Accession (Same CDS sequence)	NM_001281529.1 , NM_001281529.2
Accession Version	NM_001281529.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	proteasome activator complex subunit 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BJ993879.1, BM470561.1, AI445328.1 and BM981616.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 4 which is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM470561.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGC TAGAAGGCTT CCACACTCAA 
ATCTCTAAGT ATTTCTCTGA GCGTGGTGAT GCAGTGACTA AAGCAGCCAA GCAGCCCCAT 
GTGGGTGATT ATCGGCAGCT GGTGCACGAG CTGGATGAGG CAGAGTACCG GGACATCCGG 
CTGATGGTCA TGGAGATCCG CAATGCTTAT GCTGTGTTAT ATGACATCAT CCTGAAGAAC 
TTCGAGAAGC TCAAGAAGCC CAGGGGAGAA ACAAAGGGAA TGATCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268458.1
CDS	106..816
Translation	MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

Target ORF information:

RefSeq Version	NM_001281529.1
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281529.1

ORF Insert Sequence:

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGC TAGAAGGCTT CCACACTCAA 
ATCTCTAAGT ATTTCTCTGA GCGTGGTGAT GCAGTGACTA AAGCAGCCAA GCAGCCCCAT 
GTGGGTGATT ATCGGCAGCT GGTGCACGAG CTGGATGAGG CAGAGTACCG GGACATCCGG 
CTGATGGTCA TGGAGATCCG CAATGCTTAT GCTGTGTTAT ATGACATCAT CCTGAAGAAC 
TTCGAGAAGC TCAAGAAGCC CAGGGGAGAA ACAAAGGGAA TGATCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSME1 ( NM_001281529.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001268458.1 Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu09693
Clone ID Related Accession (Same CDS sequence)	NM_001281529.1 , NM_001281529.2
Accession Version	NM_001281529.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	proteasome activator complex subunit 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BJ993879.1, BM470561.1, AI445328.1 and BM981616.1. On May 31, 2019 this sequence version replaced NM_001281529.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. It encodes isoform 4 which is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM470561.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGC TAGAAGGCTT CCACACTCAA 
ATCTCTAAGT ATTTCTCTGA GCGTGGTGAT GCAGTGACTA AAGCAGCCAA GCAGCCCCAT 
GTGGGTGATT ATCGGCAGCT GGTGCACGAG CTGGATGAGG CAGAGTACCG GGACATCCGG 
CTGATGGTCA TGGAGATCCG CAATGCTTAT GCTGTGTTAT ATGACATCAT CCTGAAGAAC 
TTCGAGAAGC TCAAGAAGCC CAGGGGAGAA ACAAAGGGAA TGATCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268458.1
CDS	70..780
Translation	MAMLRVQPEAQAKVDVFREDLCTKTENLLGSYFPKKISELDAFLKEPALNEANLSNLKAPLDIPVPDPVKEKEKEERKKQQEKEDKDEKKKGEDEDKGPPCGPVNCNEKIVVLLQRLKPEIKDVIEQLNLVTTWLQLQIPRIEDGNNFGVAVQLEGFHTQISKYFSERGDAVTKAAKQPHVGDYRQLVHELDEAEYRDIRLMVMEIRNAYAVLYDIILKNFEKLKKPRGETKGMIY

Target ORF information:

RefSeq Version	NM_001281529.2
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome activator subunit 1 (PSME1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281529.2

ORF Insert Sequence:

ATGGCCATGC TCAGGGTCCA GCCCGAGGCC CAAGCCAAGG TGGATGTGTT TCGTGAAGAC 
CTCTGTACCA AGACAGAGAA CCTGCTCGGG AGCTATTTCC CCAAGAAGAT TTCTGAGCTG 
GATGCATTTT TAAAGGAGCC AGCTCTCAAT GAAGCCAACT TGAGCAATCT GAAGGCCCCA 
TTGGACATCC CAGTGCCTGA TCCAGTCAAG GAGAAAGAGA AAGAGGAGCG GAAGAAACAG 
CAGGAGAAGG AAGACAAGGA TGAAAAGAAG AAGGGGGAGG ATGAAGACAA AGGTCCTCCC 
TGTGGCCCAG TGAACTGCAA TGAAAAGATC GTGGTCCTTC TGCAGCGCTT GAAGCCTGAG 
ATCAAGGATG TCATTGAGCA GCTCAACCTG GTCACCACCT GGTTGCAGCT GCAGATACCT 
CGGATTGAGG ATGGTAACAA TTTTGGAGTG GCTGTCCAGC TAGAAGGCTT CCACACTCAA 
ATCTCTAAGT ATTTCTCTGA GCGTGGTGAT GCAGTGACTA AAGCAGCCAA GCAGCCCCAT 
GTGGGTGATT ATCGGCAGCT GGTGCACGAG CTGGATGAGG CAGAGTACCG GGACATCCGG 
CTGATGGTCA TGGAGATCCG CAATGCTTAT GCTGTGTTAT ATGACATCAT CCTGAAGAAC 
TTCGAGAAGC TCAAGAAGCC CAGGGGAGAA ACAAAGGGAA TGATCTATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Circadian modulation of proteasome activity and accumulation of oxidized protein in human embryonic kidney HEK 293 cells and primary dermal fibroblasts.
Free radical biology & medicine94195-207(2016 05)
Desvergne A,Ugarte N,Radjei S,Gareil M,Petropoulos I,Friguet B

Overexpression of proteasomal activator PA28? serves as a prognostic factor in oral squamous cell carcinoma.
Journal of experimental & clinical cancer research : CR3535(2016 Feb)
Feng X,Jiang Y,Xie L,Jiang L,Li J,Sun C,Xu H,Wang R,Zhou M,Zhou Y,Dan H,Wang Z,Ji N,Deng P,Liao G,Geng N,Wang Y,Zhang D,Lin Y,Ye L,Liang X,Li L,Luo G,Feng M,Fang J,Zeng X,Wang Z,Chen Q

The proteasome immunosubunits, PA28 and ER-aminopeptidase 1 protect melanoma cells from efficient MART-126-35 -specific T-cell recognition.
European journal of immunology45(12)3257-68(2015 Dec)
Keller M,Ebstein F,B?rger E,Textoris-Taube K,Gorny X,Urban S,Zhao F,Dannenberg T,Sucker A,Keller C,Saveanu L,Kr?ger E,Rothk?tter HJ,Dahlmann B,Henklein P,Voigt A,Kuckelkorn U,Paschen A,Kloetzel PM,Seifert U

Proteasome activator complex PA28 identified as an accessible target in prostate cancer by in vivo selection of human antibodies.
Proceedings of the National Academy of Sciences of the United States of America110(34)13791-6(2013 Aug)
S?nchez-Mart?n D,Mart?nez-Torrecuadrada J,Teesalu T,Sugahara KN,Alvarez-Cienfuegos A,Xim?nez-Emb?n P,Fern?ndez-Peri??ez R,Mart?n MT,Molina-Privado I,Ruppen-Ca??s I,Blanco-Toribio A,Ca?amero M,Cuesta AM,Compte M,Kremer L,Bellas C,Alonso-Camino V,Guijarro-Mu?oz I,Sanz L,Ruoslahti E,Alvarez-Vallina L

Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFN? in mouse pancreatic ?-cells.
PloS one8(2)e52408(2013)
Freudenburg W,Gautam M,Chakraborty P,James J,Richards J,Salvatori AS,Baldwin A,Schriewer J,Buller RM,Corbett JA,Skowyra D

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PSME1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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