PRKG1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_006258.3	NP_006249.1	cGMP-dependent protein kinase 1 isoform 2
NM_001374781.1	NP_001361710.1	cGMP-dependent protein kinase 1 isoform 3
XM_024448078.1	XP_024303846.1	cGMP-dependent protein kinase 1 isoform X4
XM_017016412.1	XP_016871901.1	cGMP-dependent protein kinase 1 isoform X1
XM_017016413.1	XP_016871902.1	cGMP-dependent protein kinase 1 isoform X2
NM_006258.4	NP_006249.1	cGMP-dependent protein kinase 1 isoform 2
XM_011539952.2	XP_011538254.1	cGMP-dependent protein kinase 1 isoform X3
NM_001098512.3	NP_001091982.1	cGMP-dependent protein kinase 1 isoform 1
NM_001374782.1	NP_001361711.1	cGMP-dependent protein kinase 1 isoform 4
NM_001098512.2	NP_001091982.1	cGMP-dependent protein kinase 1 isoform 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26943	NM_006258.4 Latest version!	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu59938	XM_011539952.2 Latest version!	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu106536	NM_001374781.1 Latest version!	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu108897	NM_001374782.1 Latest version!	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu106536	XM_024448078.1 Latest version!	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu26843	NM_001098512.3 Latest version!	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu91517	XM_017016412.1 Latest version!	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu91518	XM_017016413.1 Latest version!	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu26943	NM_006258.3	Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu26843	NM_001098512.2	Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

PRKG1 ( NM_006258.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006249.1 Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26943
Clone ID Related Accession (Same CDS sequence)	NM_006258.3 , NM_006258.4
Accession Version	NM_006258.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA150150.1, BC127090.1, AL599933.1, BX952280.1, DB138350.1, DA915933.1, DA646702.1, CD723759.1, BX097700.1 and BQ445850.1. This sequence is a reference standard in the RefSeqGene project. On Sep 9, 2011 this sequence version replaced NM_006258.2. Transcript Variant: This variant (2) uses an alternate exon at its 5' end which result in the use of a distinct translation initiation site, compared to variant 1. This difference results in an isoform (2; also known as beta or 1beta) with a longer and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.196058.1, SRR1803614.29052.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCACCT TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GACCAGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAGGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCCGAGCCCA CCGCCTTCGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGCCCAC AGTCCAAGGA TCTTATAAAG GAAGCTATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCGCAGAT CCAGGAGATT GTGGATTGTA TGTACCCGGT GGAGTATGGC 
AAGGACAGTT GCATCATCAA AGAAGGAGAC GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGTGTG AAGTTGTGTA CCATGGGTCC AGGAAAAGTG 
TTTGGGGAAT TGGCTATTCT TTACAACTGT ACCCGGACAG CGACCGTCAA GACTCTTGTA 
AATGTAAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAAA CAATAATGAT GAGGACAGGA 
CTCATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGCAA GCTTGCTGAT GTCCTTGAAG AGACCCACTA TGAAAATGGA 
GAATATATTA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG CAAAGGAACG 
GTAAATGTCA CTCGTGAAGA CTCACCGAGT GAAGACCCAG TCTTTCTTAG AACTTTAGGA 
AAAGGAGACT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCAAACGTA 
ATTGCTGCAG AAGCTGTAAC CTGCCTTGTG ATTGACAGAG ACTCTTTTAA ACATTTGATT 
GGAGGGCTGG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAATAT 
GAAGCTGAAG CGGCTTTCTT CGCCAACCTG AAGCTGTCTG ATTTCAACAT CATTGATACC 
CTTGGAGTTG GAGGTTTCGG ACGAGTAGAA CTGGTCCAGT TGAAAAGTGA AGAATCCAAA 
ACGTTTGCAA TGAAGATTCT CAAGAAACGT CACATTGTGG ACACAAGACA GCAGGAGCAC 
ATCCGCTCAG AGAAGCAGAT CATGCAGGGG GCTCATTCCG ATTTCATAGT GAGACTGTAC 
AGAACATTTA AGGACAGCAA ATATTTGTAT ATGTTGATGG AAGCTTGTCT AGGTGGAGAG 
CTCTGGACCA TTCTCAGGGA TAGAGGTTCG TTTGAAGATT CTACAACCAG ATTTTACACA 
GCATGTGTGG TAGAAGCTTT TGCCTATCTG CATTCCAAAG GAATCATTTA CAGGGACCTC 
AAGCCAGAAA ATCTCATCCT AGATCACCGA GGTTATGCCA AACTGGTTGA TTTTGGCTTT 
GCAAAGAAAA TAGGATTTGG AAAGAAAACA TGGACTTTTT GTGGGACTCC AGAGTATGTA 
GCCCCAGAGA TCATCCTGAA CAAAGGCCAT GACATTTCAG CCGACTACTG GTCACTGGGA 
ATCCTAATGT ATGAACTCCT GACTGGCAGC CCACCTTTCT CAGGCCCAGA TCCTATGAAA 
ACCTATAACA TCATATTGAG GGGGATTGAC ATGATAGAAT TTCCAAAGAA GATTGCCAAA 
AATGCTGCTA ATTTAATTAA AAAACTATGC AGGGACAATC CATCAGAAAG ATTAGGGAAT 
TTGAAAAATG GAGTAAAAGA CATTCAAAAG CACAAATGGT TTGAGGGCTT TAACTGGGAA 
GGCTTAAGAA AAGGTACCTT GACACCTCCT ATAATACCAA GTGTTGCATC ACCCACAGAC 
ACAAGTAATT TTGACAGTTT CCCTGAGGAC AACGATGAAC CACCACCTGA TGACAACTCA 
GGATGGGATA TAGACTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006249.1
CDS	118..2178
Translation	MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	NM_006258.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006258.3

ORF Insert Sequence:

ATGGGCACCT TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GACCAGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAGGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCCGAGCCCA CCGCCTTCGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGCCCAC AGTCCAAGGA TCTTATAAAG GAAGCTATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCGCAGAT CCAGGAGATT GTGGATTGTA TGTACCCGGT GGAGTATGGC 
AAGGACAGTT GCATCATCAA AGAAGGAGAC GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGTGTG AAGTTGTGTA CCATGGGTCC AGGAAAAGTG 
TTTGGGGAAT TGGCTATTCT TTACAACTGT ACCCGGACAG CGACCGTCAA GACTCTTGTA 
AATGTAAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAAA CAATAATGAT GAGGACAGGA 
CTCATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGCAA GCTTGCTGAT GTCCTTGAAG AGACCCACTA TGAAAATGGA 
GAATATATTA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG CAAAGGAACG 
GTAAATGTCA CTCGTGAAGA CTCACCGAGT GAAGACCCAG TCTTTCTTAG AACTTTAGGA 
AAAGGAGACT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCAAACGTA 
ATTGCTGCAG AAGCTGTAAC CTGCCTTGTG ATTGACAGAG ACTCTTTTAA ACATTTGATT 
GGAGGGCTGG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAATAT 
GAAGCTGAAG CGGCTTTCTT CGCCAACCTG AAGCTGTCTG ATTTCAACAT CATTGATACC 
CTTGGAGTTG GAGGTTTCGG ACGAGTAGAA CTGGTCCAGT TGAAAAGTGA AGAATCCAAA 
ACGTTTGCAA TGAAGATTCT CAAGAAACGT CACATTGTGG ACACAAGACA GCAGGAGCAC 
ATCCGCTCAG AGAAGCAGAT CATGCAGGGG GCTCATTCCG ATTTCATAGT GAGACTGTAC 
AGAACATTTA AGGACAGCAA ATATTTGTAT ATGTTGATGG AAGCTTGTCT AGGTGGAGAG 
CTCTGGACCA TTCTCAGGGA TAGAGGTTCG TTTGAAGATT CTACAACCAG ATTTTACACA 
GCATGTGTGG TAGAAGCTTT TGCCTATCTG CATTCCAAAG GAATCATTTA CAGGGACCTC 
AAGCCAGAAA ATCTCATCCT AGATCACCGA GGTTATGCCA AACTGGTTGA TTTTGGCTTT 
GCAAAGAAAA TAGGATTTGG AAAGAAAACA TGGACTTTTT GTGGGACTCC AGAGTATGTA 
GCCCCAGAGA TCATCCTGAA CAAAGGCCAT GACATTTCAG CCGACTACTG GTCACTGGGA 
ATCCTAATGT ATGAACTCCT GACTGGCAGC CCACCTTTCT CAGGCCCAGA TCCTATGAAA 
ACCTATAACA TCATATTGAG GGGGATTGAC ATGATAGAAT TTCCAAAGAA GATTGCCAAA 
AATGCTGCTA ATTTAATTAA AAAACTATGC AGGGACAATC CATCAGAAAG ATTAGGGAAT 
TTGAAAAATG GAGTAAAAGA CATTCAAAAG CACAAATGGT TTGAGGGCTT TAACTGGGAA 
GGCTTAAGAA AAGGTACCTT GACACCTCCT ATAATACCAA GTGTTGCATC ACCCACAGAC 
ACAAGTAATT TTGACAGTTT CCCTGAGGAC AACGATGAAC CACCACCTGA TGACAACTCA 
GGATGGGATA TAGACTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( NM_006258.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006249.1 Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26943
Clone ID Related Accession (Same CDS sequence)	NM_006258.3 , NM_006258.4
Accession Version	NM_006258.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-15
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA150150.1, BC127090.1, AL599933.1, BX952280.1, DB138350.1, DA915933.1, DA646702.1, CD723759.1, BX097700.1 and BQ445850.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2018 this sequence version replaced NM_006258.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.196058.1, SRR1803614.29052.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCACCT TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GACCAGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAGGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCCGAGCCCA CCGCCTTCGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGCCCAC AGTCCAAGGA TCTTATAAAG GAAGCTATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCGCAGAT CCAGGAGATT GTGGATTGTA TGTACCCGGT GGAGTATGGC 
AAGGACAGTT GCATCATCAA AGAAGGAGAC GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGTGTG AAGTTGTGTA CCATGGGTCC AGGAAAAGTG 
TTTGGGGAAT TGGCTATTCT TTACAACTGT ACCCGGACAG CGACCGTCAA GACTCTTGTA 
AATGTAAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAAA CAATAATGAT GAGGACAGGA 
CTCATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGCAA GCTTGCTGAT GTCCTTGAAG AGACCCACTA TGAAAATGGA 
GAATATATTA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG CAAAGGAACG 
GTAAATGTCA CTCGTGAAGA CTCACCGAGT GAAGACCCAG TCTTTCTTAG AACTTTAGGA 
AAAGGAGACT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCAAACGTA 
ATTGCTGCAG AAGCTGTAAC CTGCCTTGTG ATTGACAGAG ACTCTTTTAA ACATTTGATT 
GGAGGGCTGG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAATAT 
GAAGCTGAAG CGGCTTTCTT CGCCAACCTG AAGCTGTCTG ATTTCAACAT CATTGATACC 
CTTGGAGTTG GAGGTTTCGG ACGAGTAGAA CTGGTCCAGT TGAAAAGTGA AGAATCCAAA 
ACGTTTGCAA TGAAGATTCT CAAGAAACGT CACATTGTGG ACACAAGACA GCAGGAGCAC 
ATCCGCTCAG AGAAGCAGAT CATGCAGGGG GCTCATTCCG ATTTCATAGT GAGACTGTAC 
AGAACATTTA AGGACAGCAA ATATTTGTAT ATGTTGATGG AAGCTTGTCT AGGTGGAGAG 
CTCTGGACCA TTCTCAGGGA TAGAGGTTCG TTTGAAGATT CTACAACCAG ATTTTACACA 
GCATGTGTGG TAGAAGCTTT TGCCTATCTG CATTCCAAAG GAATCATTTA CAGGGACCTC 
AAGCCAGAAA ATCTCATCCT AGATCACCGA GGTTATGCCA AACTGGTTGA TTTTGGCTTT 
GCAAAGAAAA TAGGATTTGG AAAGAAAACA TGGACTTTTT GTGGGACTCC AGAGTATGTA 
GCCCCAGAGA TCATCCTGAA CAAAGGCCAT GACATTTCAG CCGACTACTG GTCACTGGGA 
ATCCTAATGT ATGAACTCCT GACTGGCAGC CCACCTTTCT CAGGCCCAGA TCCTATGAAA 
ACCTATAACA TCATATTGAG GGGGATTGAC ATGATAGAAT TTCCAAAGAA GATTGCCAAA 
AATGCTGCTA ATTTAATTAA AAAACTATGC AGGGACAATC CATCAGAAAG ATTAGGGAAT 
TTGAAAAATG GAGTAAAAGA CATTCAAAAG CACAAATGGT TTGAGGGCTT TAACTGGGAA 
GGCTTAAGAA AAGGTACCTT GACACCTCCT ATAATACCAA GTGTTGCATC ACCCACAGAC 
ACAAGTAATT TTGACAGTTT CCCTGAGGAC AACGATGAAC CACCACCTGA TGACAACTCA 
GGATGGGATA TAGACTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006249.1
CDS	105..2165
Translation	MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	NM_006258.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006258.4

ORF Insert Sequence:

ATGGGCACCT TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GACCAGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAGGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCCGAGCCCA CCGCCTTCGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGCCCAC AGTCCAAGGA TCTTATAAAG GAAGCTATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCGCAGAT CCAGGAGATT GTGGATTGTA TGTACCCGGT GGAGTATGGC 
AAGGACAGTT GCATCATCAA AGAAGGAGAC GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGTGTG AAGTTGTGTA CCATGGGTCC AGGAAAAGTG 
TTTGGGGAAT TGGCTATTCT TTACAACTGT ACCCGGACAG CGACCGTCAA GACTCTTGTA 
AATGTAAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAAA CAATAATGAT GAGGACAGGA 
CTCATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGCAA GCTTGCTGAT GTCCTTGAAG AGACCCACTA TGAAAATGGA 
GAATATATTA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG CAAAGGAACG 
GTAAATGTCA CTCGTGAAGA CTCACCGAGT GAAGACCCAG TCTTTCTTAG AACTTTAGGA 
AAAGGAGACT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCAAACGTA 
ATTGCTGCAG AAGCTGTAAC CTGCCTTGTG ATTGACAGAG ACTCTTTTAA ACATTTGATT 
GGAGGGCTGG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAATAT 
GAAGCTGAAG CGGCTTTCTT CGCCAACCTG AAGCTGTCTG ATTTCAACAT CATTGATACC 
CTTGGAGTTG GAGGTTTCGG ACGAGTAGAA CTGGTCCAGT TGAAAAGTGA AGAATCCAAA 
ACGTTTGCAA TGAAGATTCT CAAGAAACGT CACATTGTGG ACACAAGACA GCAGGAGCAC 
ATCCGCTCAG AGAAGCAGAT CATGCAGGGG GCTCATTCCG ATTTCATAGT GAGACTGTAC 
AGAACATTTA AGGACAGCAA ATATTTGTAT ATGTTGATGG AAGCTTGTCT AGGTGGAGAG 
CTCTGGACCA TTCTCAGGGA TAGAGGTTCG TTTGAAGATT CTACAACCAG ATTTTACACA 
GCATGTGTGG TAGAAGCTTT TGCCTATCTG CATTCCAAAG GAATCATTTA CAGGGACCTC 
AAGCCAGAAA ATCTCATCCT AGATCACCGA GGTTATGCCA AACTGGTTGA TTTTGGCTTT 
GCAAAGAAAA TAGGATTTGG AAAGAAAACA TGGACTTTTT GTGGGACTCC AGAGTATGTA 
GCCCCAGAGA TCATCCTGAA CAAAGGCCAT GACATTTCAG CCGACTACTG GTCACTGGGA 
ATCCTAATGT ATGAACTCCT GACTGGCAGC CCACCTTTCT CAGGCCCAGA TCCTATGAAA 
ACCTATAACA TCATATTGAG GGGGATTGAC ATGATAGAAT TTCCAAAGAA GATTGCCAAA 
AATGCTGCTA ATTTAATTAA AAAACTATGC AGGGACAATC CATCAGAAAG ATTAGGGAAT 
TTGAAAAATG GAGTAAAAGA CATTCAAAAG CACAAATGGT TTGAGGGCTT TAACTGGGAA 
GGCTTAAGAA AAGGTACCTT GACACCTCCT ATAATACCAA GTGTTGCATC ACCCACAGAC 
ACAAGTAATT TTGACAGTTT CCCTGAGGAC AACGATGAAC CACCACCTGA TGACAACTCA 
GGATGGGATA TAGACTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( XM_011539952.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538254.1 Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu59938
Clone ID Related Accession (Same CDS sequence)	XM_011539952.2
Accession Version	XM_011539952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1221bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539952.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCT TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GACCAGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAGGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCCGAGCCCA CCGCCTTCGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGCCCAC AGTCCAAGGA TCTTATAAAG GAAGCTATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCGCAGAT CCAGGAGATT GTGGATTGTA TGTACCCGGT GGAGTATGGC 
AAGGACAGTT GCATCATCAA AGAAGGAGAC GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGTGTG AAGTTGTGTA CCATGGGTCC AGGAAAAGTG 
TTTGGGGAAT TGGCTATTCT TTACAACTGT ACCCGGACAG CGACCGTCAA GACTCTTGTA 
AATGTAAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAAA CAATAATGAT GAGGACAGGA 
CTCATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGCAA GCTTGCTGAT GTCCTTGAAG AGACCCACTA TGAAAATGGA 
GAATATATTA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG CAAAGGAACG 
GTAAATGTCA CTCGTGAAGA CTCACCGAGT GAAGACCCAG TCTTTCTTAG AACTTTAGGA 
AAAGGAGACT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCAAACGTA 
ATTGCTGCAG AAGCTGTAAC CTGCCTTGTG ATTGACAGAG ACTCTTTTAA ACATTTGATT 
GGAGGGCTGG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAGTTC 
ATCAGGGAGC ATAATGCTGG CATCTGCTAC TGCTGTGGCC CTCAGAAAGC TTCCAATTAT 
GGCAGAAGGC AAAGAAGGAG CAGGCATATC ACATGGCAAG AGCAGAAACA AGAGAGGGTG 
GGAGAGGTCC CAGACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538254.1
CDS	462..1682
Translation	MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKFIREHNAGICYCCGPQKASNYGRRQRRSRHITWQEQKQERVGEVPDF

Target ORF information:

RefSeq Version	XM_011539952.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011539952.2

ORF Insert Sequence:

ATGGGCACCT TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GACCAGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAGGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCCGAGCCCA CCGCCTTCGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGCCCAC AGTCCAAGGA TCTTATAAAG GAAGCTATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCGCAGAT CCAGGAGATT GTGGATTGTA TGTACCCGGT GGAGTATGGC 
AAGGACAGTT GCATCATCAA AGAAGGAGAC GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGTGTG AAGTTGTGTA CCATGGGTCC AGGAAAAGTG 
TTTGGGGAAT TGGCTATTCT TTACAACTGT ACCCGGACAG CGACCGTCAA GACTCTTGTA 
AATGTAAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAAA CAATAATGAT GAGGACAGGA 
CTCATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGCAA GCTTGCTGAT GTCCTTGAAG AGACCCACTA TGAAAATGGA 
GAATATATTA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG CAAAGGAACG 
GTAAATGTCA CTCGTGAAGA CTCACCGAGT GAAGACCCAG TCTTTCTTAG AACTTTAGGA 
AAAGGAGACT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCAAACGTA 
ATTGCTGCAG AAGCTGTAAC CTGCCTTGTG ATTGACAGAG ACTCTTTTAA ACATTTGATT 
GGAGGGCTGG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAGTTC 
ATCAGGGAGC ATAATGCTGG CATCTGCTAC TGCTGTGGCC CTCAGAAAGC TTCCAATTAT 
GGCAGAAGGC AAAGAAGGAG CAGGCATATC ACATGGCAAG AGCAGAAACA AGAGAGGGTG 
GGAGAGGTCC CAGACTTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( NM_001374781.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361710.1 Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 3, mRNA.

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CloneID	OHu106536
Clone ID Related Accession (Same CDS sequence)	NM_001374781.1 , XM_024448078.1
Accession Version	NM_001374781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-14
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022025.8, AC027118.9, AL928686.2 and AC009986.10. On Oct 15, 2019 this sequence version replaced XM_024448078.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.197021.1 [ECO:0000332] ##Evidence-Data-END##

ATGAAGATTC TCAAGAAACG TCACATTGTG GACACAAGAC AGCAGGAGCA CATCCGCTCA 
GAGAAGCAGA TCATGCAGGG GGCTCATTCC GATTTCATAG TGAGACTGTA CAGAACATTT 
AAGGACAGCA AATATTTGTA TATGTTGATG GAAGCTTGTC TAGGTGGAGA GCTCTGGACC 
ATTCTCAGGG ATAGAGGTTC GTTTGAAGAT TCTACAACCA GATTTTACAC AGCATGTGTG 
GTAGAAGCTT TTGCCTATCT GCATTCCAAA GGAATCATTT ACAGGGACCT CAAGCCAGAA 
AATCTCATCC TAGATCACCG AGGTTATGCC AAACTGGTTG ATTTTGGCTT TGCAAAGAAA 
ATAGGATTTG GAAAGAAAAC ATGGACTTTT TGTGGGACTC CAGAGTATGT AGCCCCAGAG 
ATCATCCTGA ACAAAGGCCA TGACATTTCA GCCGACTACT GGTCACTGGG AATCCTAATG 
TATGAACTCC TGACTGGCAG CCCACCTTTC TCAGGCCCAG ATCCTATGAA AACCTATAAC 
ATCATATTGA GGGGGATTGA CATGATAGAA TTTCCAAAGA AGATTGCCAA AAATGCTGCT 
AATTTAATTA AAAAACTATG CAGGGACAAT CCATCAGAAA GATTAGGGAA TTTGAAAAAT 
GGAGTAAAAG ACATTCAAAA GCACAAATGG TTTGAGGGCT TTAACTGGGA AGGCTTAAGA 
AAAGGTACCT TGACACCTCC TATAATACCA AGTGTTGCAT CACCCACAGA CACAAGTAAT 
TTTGACAGTT TCCCTGAGGA CAACGATGAA CCACCACCTG ATGACAACTC AGGATGGGAT 
ATAGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361710.1
CDS	619..1470
Translation	MKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	NM_001374781.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374781.1

ORF Insert Sequence:

ATGAAGATTC TCAAGAAACG TCACATTGTG GACACAAGAC AGCAGGAGCA CATCCGCTCA 
GAGAAGCAGA TCATGCAGGG GGCTCATTCC GATTTCATAG TGAGACTGTA CAGAACATTT 
AAGGACAGCA AATATTTGTA TATGTTGATG GAAGCTTGTC TAGGTGGAGA GCTCTGGACC 
ATTCTCAGGG ATAGAGGTTC GTTTGAAGAT TCTACAACCA GATTTTACAC AGCATGTGTG 
GTAGAAGCTT TTGCCTATCT GCATTCCAAA GGAATCATTT ACAGGGACCT CAAGCCAGAA 
AATCTCATCC TAGATCACCG AGGTTATGCC AAACTGGTTG ATTTTGGCTT TGCAAAGAAA 
ATAGGATTTG GAAAGAAAAC ATGGACTTTT TGTGGGACTC CAGAGTATGT AGCCCCAGAG 
ATCATCCTGA ACAAAGGCCA TGACATTTCA GCCGACTACT GGTCACTGGG AATCCTAATG 
TATGAACTCC TGACTGGCAG CCCACCTTTC TCAGGCCCAG ATCCTATGAA AACCTATAAC 
ATCATATTGA GGGGGATTGA CATGATAGAA TTTCCAAAGA AGATTGCCAA AAATGCTGCT 
AATTTAATTA AAAAACTATG CAGGGACAAT CCATCAGAAA GATTAGGGAA TTTGAAAAAT 
GGAGTAAAAG ACATTCAAAA GCACAAATGG TTTGAGGGCT TTAACTGGGA AGGCTTAAGA 
AAAGGTACCT TGACACCTCC TATAATACCA AGTGTTGCAT CACCCACAGA CACAAGTAAT 
TTTGACAGTT TCCCTGAGGA CAACGATGAA CCACCACCTG ATGACAACTC AGGATGGGAT 
ATAGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( NM_001374782.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001361711.1 Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 4, mRNA.

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CloneID	OHu108897
Clone ID Related Accession (Same CDS sequence)	NM_001374782.1
Accession Version	NM_001374782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-14
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731537.8, AC022537.8, AL157399.14, AL391378.10, AC026228.6 and AC022025.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC062688.1 [ECO:0000332] ##Evidence-Data-END##

ATGGGCACCT TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GACCAGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAGGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCCGAGCCCA CCGCCTTCGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGCCCAC AGTCCAAGGA TCTTATAAAG GAAGCTATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCGCAGAT CCAGGAGATT GTGGATTGTA TGTACCCGGT GGAGTATGGC 
AAGGACAGTT GCATCATCAA AGAAGGAGAC GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGTGTG AAGTTGTGTA CCATGGGTCC AGGAAAAGTG 
TTTGGGGAAT TGGCTATTCT TTACAACTGT ACCCGGACAG CGACCGTCAA GACTCTTGTA 
AATGTAAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAAA CAATAATGAT GAGGACAGGA 
CTCATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGCAA GCTTGCTGAT GTCCTTGAAG AGACCCACTA TGAAAATGGA 
GAATATATTA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG CAAAGGAACG 
GTAAATGTCA CTCGTGAAGA CTCACCGAGT GAAGACCCAG TCTTTCTTAG AACTTTAGGA 
AAAGGAGACT GGTTTGGAGA GAAAGCCTTG CAGGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001361711.1
CDS	105..1043
Translation	MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQG

Target ORF information:

RefSeq Version	NM_001374782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001374782.1

ORF Insert Sequence:

ATGGGCACCT TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GACCAGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAGGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCCGAGCCCA CCGCCTTCGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGCCCAC AGTCCAAGGA TCTTATAAAG GAAGCTATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCGCAGAT CCAGGAGATT GTGGATTGTA TGTACCCGGT GGAGTATGGC 
AAGGACAGTT GCATCATCAA AGAAGGAGAC GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGTGTG AAGTTGTGTA CCATGGGTCC AGGAAAAGTG 
TTTGGGGAAT TGGCTATTCT TTACAACTGT ACCCGGACAG CGACCGTCAA GACTCTTGTA 
AATGTAAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAAA CAATAATGAT GAGGACAGGA 
CTCATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGCAA GCTTGCTGAT GTCCTTGAAG AGACCCACTA TGAAAATGGA 
GAATATATTA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG CAAAGGAACG 
GTAAATGTCA CTCGTGAAGA CTCACCGAGT GAAGACCCAG TCTTTCTTAG AACTTTAGGA 
AAAGGAGACT GGTTTGGAGA GAAAGCCTTG CAGGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( XM_024448078.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303846.1 Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu106536
Clone ID Related Accession (Same CDS sequence)	NM_001374781.1 , XM_024448078.1
Accession Version	XM_024448078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAAGATTC TCAAGAAACG TCACATTGTG GACACAAGAC AGCAGGAGCA CATCCGCTCA 
GAGAAGCAGA TCATGCAGGG GGCTCATTCC GATTTCATAG TGAGACTGTA CAGAACATTT 
AAGGACAGCA AATATTTGTA TATGTTGATG GAAGCTTGTC TAGGTGGAGA GCTCTGGACC 
ATTCTCAGGG ATAGAGGTTC GTTTGAAGAT TCTACAACCA GATTTTACAC AGCATGTGTG 
GTAGAAGCTT TTGCCTATCT GCATTCCAAA GGAATCATTT ACAGGGACCT CAAGCCAGAA 
AATCTCATCC TAGATCACCG AGGTTATGCC AAACTGGTTG ATTTTGGCTT TGCAAAGAAA 
ATAGGATTTG GAAAGAAAAC ATGGACTTTT TGTGGGACTC CAGAGTATGT AGCCCCAGAG 
ATCATCCTGA ACAAAGGCCA TGACATTTCA GCCGACTACT GGTCACTGGG AATCCTAATG 
TATGAACTCC TGACTGGCAG CCCACCTTTC TCAGGCCCAG ATCCTATGAA AACCTATAAC 
ATCATATTGA GGGGGATTGA CATGATAGAA TTTCCAAAGA AGATTGCCAA AAATGCTGCT 
AATTTAATTA AAAAACTATG CAGGGACAAT CCATCAGAAA GATTAGGGAA TTTGAAAAAT 
GGAGTAAAAG ACATTCAAAA GCACAAATGG TTTGAGGGCT TTAACTGGGA AGGCTTAAGA 
AAAGGTACCT TGACACCTCC TATAATACCA AGTGTTGCAT CACCCACAGA CACAAGTAAT 
TTTGACAGTT TCCCTGAGGA CAACGATGAA CCACCACCTG ATGACAACTC AGGATGGGAT 
ATAGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303846.1
CDS	826..1677
Translation	MKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	XM_024448078.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448078.1

ORF Insert Sequence:

ATGAAGATTC TCAAGAAACG TCACATTGTG GACACAAGAC AGCAGGAGCA CATCCGCTCA 
GAGAAGCAGA TCATGCAGGG GGCTCATTCC GATTTCATAG TGAGACTGTA CAGAACATTT 
AAGGACAGCA AATATTTGTA TATGTTGATG GAAGCTTGTC TAGGTGGAGA GCTCTGGACC 
ATTCTCAGGG ATAGAGGTTC GTTTGAAGAT TCTACAACCA GATTTTACAC AGCATGTGTG 
GTAGAAGCTT TTGCCTATCT GCATTCCAAA GGAATCATTT ACAGGGACCT CAAGCCAGAA 
AATCTCATCC TAGATCACCG AGGTTATGCC AAACTGGTTG ATTTTGGCTT TGCAAAGAAA 
ATAGGATTTG GAAAGAAAAC ATGGACTTTT TGTGGGACTC CAGAGTATGT AGCCCCAGAG 
ATCATCCTGA ACAAAGGCCA TGACATTTCA GCCGACTACT GGTCACTGGG AATCCTAATG 
TATGAACTCC TGACTGGCAG CCCACCTTTC TCAGGCCCAG ATCCTATGAA AACCTATAAC 
ATCATATTGA GGGGGATTGA CATGATAGAA TTTCCAAAGA AGATTGCCAA AAATGCTGCT 
AATTTAATTA AAAAACTATG CAGGGACAAT CCATCAGAAA GATTAGGGAA TTTGAAAAAT 
GGAGTAAAAG ACATTCAAAA GCACAAATGG TTTGAGGGCT TTAACTGGGA AGGCTTAAGA 
AAAGGTACCT TGACACCTCC TATAATACCA AGTGTTGCAT CACCCACAGA CACAAGTAAT 
TTTGACAGTT TCCCTGAGGA CAACGATGAA CCACCACCTG ATGACAACTC AGGATGGGAT 
ATAGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( NM_001098512.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091982.1 Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26843
Clone ID Related Accession (Same CDS sequence)	NM_001098512.3 , NM_001098512.2
Accession Version	NM_001098512.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX117079.1, AL731537.8, DC381245.1, D45864.1, BC127090.1, AL599933.1, BX952280.1, DB138350.1, DA915933.1, DA646702.1, CD723759.1, BX097700.1 and BQ445850.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2018 this sequence version replaced NM_001098512.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D45864.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGC TAGAGGAAGA CTTTGCCAAG ATTCTCATGC TCAAGGAGGA GAGGATCAAA 
GAGCTGGAGA AGCGGCTGTC AGAGAAGGAG GAAGAAATTC AGGAGCTGAA GAGGAAACTC 
CACAAATGCC AGTCGGTGCT CCCAGTGCCC TCGACCCACA TCGGCCCCCG GACCACCCGG 
GCGCAGGGCA TCTCGGCCGA GCCGCAGACG TACAGGTCCT TCCACGACCT CCGACAGGCA 
TTCCGGAAGT TCACCAAGTC CGAAAGGTCC AAGGATCTTA TAAAGGAAGC TATCCTTGAC 
AATGACTTTA TGAAGAACTT GGAGCTGTCG CAGATCCAGG AGATTGTGGA TTGTATGTAC 
CCGGTGGAGT ATGGCAAGGA CAGTTGCATC ATCAAAGAAG GAGACGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGTAA GGTTGAAGTT ACAAAAGAAG GTGTGAAGTT GTGTACCATG 
GGTCCAGGAA AAGTGTTTGG GGAATTGGCT ATTCTTTACA ACTGTACCCG GACAGCGACC 
GTCAAGACTC TTGTAAATGT AAAACTCTGG GCCATTGATC GACAATGTTT TCAAACAATA 
ATGATGAGGA CAGGACTCAT CAAGCATACC GAGTATATGG AATTTTTAAA AAGCGTTCCA 
ACATTCCAGA GCCTTCCTGA AGAGATCCTC AGCAAGCTTG CTGATGTCCT TGAAGAGACC 
CACTATGAAA ATGGAGAATA TATTATCAGG CAAGGTGCAA GAGGGGACAC CTTCTTTATC 
ATCAGCAAAG GAACGGTAAA TGTCACTCGT GAAGACTCAC CGAGTGAAGA CCCAGTCTTT 
CTTAGAACTT TAGGAAAAGG AGACTGGTTT GGAGAGAAAG CCTTGCAGGG GGAAGATGTG 
AGAACAGCAA ACGTAATTGC TGCAGAAGCT GTAACCTGCC TTGTGATTGA CAGAGACTCT 
TTTAAACATT TGATTGGAGG GCTGGATGAT GTTTCTAATA AAGCATATGA AGATGCAGAA 
GCTAAAGCAA AATATGAAGC TGAAGCGGCT TTCTTCGCCA ACCTGAAGCT GTCTGATTTC 
AACATCATTG ATACCCTTGG AGTTGGAGGT TTCGGACGAG TAGAACTGGT CCAGTTGAAA 
AGTGAAGAAT CCAAAACGTT TGCAATGAAG ATTCTCAAGA AACGTCACAT TGTGGACACA 
AGACAGCAGG AGCACATCCG CTCAGAGAAG CAGATCATGC AGGGGGCTCA TTCCGATTTC 
ATAGTGAGAC TGTACAGAAC ATTTAAGGAC AGCAAATATT TGTATATGTT GATGGAAGCT 
TGTCTAGGTG GAGAGCTCTG GACCATTCTC AGGGATAGAG GTTCGTTTGA AGATTCTACA 
ACCAGATTTT ACACAGCATG TGTGGTAGAA GCTTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC AGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGATTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACTCCAGAGT ATGTAGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCAGCCGAC 
TACTGGTCAC TGGGAATCCT AATGTATGAA CTCCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA TAACATCATA TTGAGGGGGA TTGACATGAT AGAATTTCCA 
AAGAAGATTG CCAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA CAATCCATCA 
GAAAGATTAG GGAATTTGAA AAATGGAGTA AAAGACATTC AAAAGCACAA ATGGTTTGAG 
GGCTTTAACT GGGAAGGCTT AAGAAAAGGT ACCTTGACAC CTCCTATAAT ACCAAGTGTT 
GCATCACCCA CAGACACAAG TAATTTTGAC AGTTTCCCTG AGGACAACGA TGAACCACCA 
CCTGATGACA ACTCAGGATG GGATATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091982.1
CDS	492..2507
Translation	MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	NM_001098512.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098512.3

ORF Insert Sequence:

ATGAGCGAGC TAGAGGAAGA CTTTGCCAAG ATTCTCATGC TCAAGGAGGA GAGGATCAAA 
GAGCTGGAGA AGCGGCTGTC AGAGAAGGAG GAAGAAATTC AGGAGCTGAA GAGGAAACTC 
CACAAATGCC AGTCGGTGCT CCCAGTGCCC TCGACCCACA TCGGCCCCCG GACCACCCGG 
GCGCAGGGCA TCTCGGCCGA GCCGCAGACG TACAGGTCCT TCCACGACCT CCGACAGGCA 
TTCCGGAAGT TCACCAAGTC CGAAAGGTCC AAGGATCTTA TAAAGGAAGC TATCCTTGAC 
AATGACTTTA TGAAGAACTT GGAGCTGTCG CAGATCCAGG AGATTGTGGA TTGTATGTAC 
CCGGTGGAGT ATGGCAAGGA CAGTTGCATC ATCAAAGAAG GAGACGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGTAA GGTTGAAGTT ACAAAAGAAG GTGTGAAGTT GTGTACCATG 
GGTCCAGGAA AAGTGTTTGG GGAATTGGCT ATTCTTTACA ACTGTACCCG GACAGCGACC 
GTCAAGACTC TTGTAAATGT AAAACTCTGG GCCATTGATC GACAATGTTT TCAAACAATA 
ATGATGAGGA CAGGACTCAT CAAGCATACC GAGTATATGG AATTTTTAAA AAGCGTTCCA 
ACATTCCAGA GCCTTCCTGA AGAGATCCTC AGCAAGCTTG CTGATGTCCT TGAAGAGACC 
CACTATGAAA ATGGAGAATA TATTATCAGG CAAGGTGCAA GAGGGGACAC CTTCTTTATC 
ATCAGCAAAG GAACGGTAAA TGTCACTCGT GAAGACTCAC CGAGTGAAGA CCCAGTCTTT 
CTTAGAACTT TAGGAAAAGG AGACTGGTTT GGAGAGAAAG CCTTGCAGGG GGAAGATGTG 
AGAACAGCAA ACGTAATTGC TGCAGAAGCT GTAACCTGCC TTGTGATTGA CAGAGACTCT 
TTTAAACATT TGATTGGAGG GCTGGATGAT GTTTCTAATA AAGCATATGA AGATGCAGAA 
GCTAAAGCAA AATATGAAGC TGAAGCGGCT TTCTTCGCCA ACCTGAAGCT GTCTGATTTC 
AACATCATTG ATACCCTTGG AGTTGGAGGT TTCGGACGAG TAGAACTGGT CCAGTTGAAA 
AGTGAAGAAT CCAAAACGTT TGCAATGAAG ATTCTCAAGA AACGTCACAT TGTGGACACA 
AGACAGCAGG AGCACATCCG CTCAGAGAAG CAGATCATGC AGGGGGCTCA TTCCGATTTC 
ATAGTGAGAC TGTACAGAAC ATTTAAGGAC AGCAAATATT TGTATATGTT GATGGAAGCT 
TGTCTAGGTG GAGAGCTCTG GACCATTCTC AGGGATAGAG GTTCGTTTGA AGATTCTACA 
ACCAGATTTT ACACAGCATG TGTGGTAGAA GCTTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC AGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGATTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACTCCAGAGT ATGTAGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCAGCCGAC 
TACTGGTCAC TGGGAATCCT AATGTATGAA CTCCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA TAACATCATA TTGAGGGGGA TTGACATGAT AGAATTTCCA 
AAGAAGATTG CCAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA CAATCCATCA 
GAAAGATTAG GGAATTTGAA AAATGGAGTA AAAGACATTC AAAAGCACAA ATGGTTTGAG 
GGCTTTAACT GGGAAGGCTT AAGAAAAGGT ACCTTGACAC CTCCTATAAT ACCAAGTGTT 
GCATCACCCA CAGACACAAG TAATTTTGAC AGTTTCCCTG AGGACAACGA TGAACCACCA 
CCTGATGACA ACTCAGGATG GGATATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( NM_001098512.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091982.1 Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 1, mRNA.

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CloneID	OHu26843
Clone ID Related Accession (Same CDS sequence)	NM_001098512.3 , NM_001098512.2
Accession Version	NM_001098512.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC381245.1, D45864.1, BC127090.1, AL599933.1, BX952280.1, DB138350.1, DA915933.1, DA646702.1, CD723759.1, BX097700.1 and BQ445850.1. This sequence is a reference standard in the RefSeqGene project. On Sep 9, 2011 this sequence version replaced NM_001098512.1. Transcript Variant: This variant (1) encodes the shorter isoform (1; also known as alpha or 1alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D45864.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGC TAGAGGAAGA CTTTGCCAAG ATTCTCATGC TCAAGGAGGA GAGGATCAAA 
GAGCTGGAGA AGCGGCTGTC AGAGAAGGAG GAAGAAATTC AGGAGCTGAA GAGGAAACTC 
CACAAATGCC AGTCGGTGCT CCCAGTGCCC TCGACCCACA TCGGCCCCCG GACCACCCGG 
GCGCAGGGCA TCTCGGCCGA GCCGCAGACG TACAGGTCCT TCCACGACCT CCGACAGGCA 
TTCCGGAAGT TCACCAAGTC CGAAAGGTCC AAGGATCTTA TAAAGGAAGC TATCCTTGAC 
AATGACTTTA TGAAGAACTT GGAGCTGTCG CAGATCCAGG AGATTGTGGA TTGTATGTAC 
CCGGTGGAGT ATGGCAAGGA CAGTTGCATC ATCAAAGAAG GAGACGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGTAA GGTTGAAGTT ACAAAAGAAG GTGTGAAGTT GTGTACCATG 
GGTCCAGGAA AAGTGTTTGG GGAATTGGCT ATTCTTTACA ACTGTACCCG GACAGCGACC 
GTCAAGACTC TTGTAAATGT AAAACTCTGG GCCATTGATC GACAATGTTT TCAAACAATA 
ATGATGAGGA CAGGACTCAT CAAGCATACC GAGTATATGG AATTTTTAAA AAGCGTTCCA 
ACATTCCAGA GCCTTCCTGA AGAGATCCTC AGCAAGCTTG CTGATGTCCT TGAAGAGACC 
CACTATGAAA ATGGAGAATA TATTATCAGG CAAGGTGCAA GAGGGGACAC CTTCTTTATC 
ATCAGCAAAG GAACGGTAAA TGTCACTCGT GAAGACTCAC CGAGTGAAGA CCCAGTCTTT 
CTTAGAACTT TAGGAAAAGG AGACTGGTTT GGAGAGAAAG CCTTGCAGGG GGAAGATGTG 
AGAACAGCAA ACGTAATTGC TGCAGAAGCT GTAACCTGCC TTGTGATTGA CAGAGACTCT 
TTTAAACATT TGATTGGAGG GCTGGATGAT GTTTCTAATA AAGCATATGA AGATGCAGAA 
GCTAAAGCAA AATATGAAGC TGAAGCGGCT TTCTTCGCCA ACCTGAAGCT GTCTGATTTC 
AACATCATTG ATACCCTTGG AGTTGGAGGT TTCGGACGAG TAGAACTGGT CCAGTTGAAA 
AGTGAAGAAT CCAAAACGTT TGCAATGAAG ATTCTCAAGA AACGTCACAT TGTGGACACA 
AGACAGCAGG AGCACATCCG CTCAGAGAAG CAGATCATGC AGGGGGCTCA TTCCGATTTC 
ATAGTGAGAC TGTACAGAAC ATTTAAGGAC AGCAAATATT TGTATATGTT GATGGAAGCT 
TGTCTAGGTG GAGAGCTCTG GACCATTCTC AGGGATAGAG GTTCGTTTGA AGATTCTACA 
ACCAGATTTT ACACAGCATG TGTGGTAGAA GCTTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC AGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGATTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACTCCAGAGT ATGTAGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCAGCCGAC 
TACTGGTCAC TGGGAATCCT AATGTATGAA CTCCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA TAACATCATA TTGAGGGGGA TTGACATGAT AGAATTTCCA 
AAGAAGATTG CCAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA CAATCCATCA 
GAAAGATTAG GGAATTTGAA AAATGGAGTA AAAGACATTC AAAAGCACAA ATGGTTTGAG 
GGCTTTAACT GGGAAGGCTT AAGAAAAGGT ACCTTGACAC CTCCTATAAT ACCAAGTGTT 
GCATCACCCA CAGACACAAG TAATTTTGAC AGTTTCCCTG AGGACAACGA TGAACCACCA 
CCTGATGACA ACTCAGGATG GGATATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091982.1
CDS	229..2244
Translation	MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	NM_001098512.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001098512.2

ORF Insert Sequence:

ATGAGCGAGC TAGAGGAAGA CTTTGCCAAG ATTCTCATGC TCAAGGAGGA GAGGATCAAA 
GAGCTGGAGA AGCGGCTGTC AGAGAAGGAG GAAGAAATTC AGGAGCTGAA GAGGAAACTC 
CACAAATGCC AGTCGGTGCT CCCAGTGCCC TCGACCCACA TCGGCCCCCG GACCACCCGG 
GCGCAGGGCA TCTCGGCCGA GCCGCAGACG TACAGGTCCT TCCACGACCT CCGACAGGCA 
TTCCGGAAGT TCACCAAGTC CGAAAGGTCC AAGGATCTTA TAAAGGAAGC TATCCTTGAC 
AATGACTTTA TGAAGAACTT GGAGCTGTCG CAGATCCAGG AGATTGTGGA TTGTATGTAC 
CCGGTGGAGT ATGGCAAGGA CAGTTGCATC ATCAAAGAAG GAGACGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGTAA GGTTGAAGTT ACAAAAGAAG GTGTGAAGTT GTGTACCATG 
GGTCCAGGAA AAGTGTTTGG GGAATTGGCT ATTCTTTACA ACTGTACCCG GACAGCGACC 
GTCAAGACTC TTGTAAATGT AAAACTCTGG GCCATTGATC GACAATGTTT TCAAACAATA 
ATGATGAGGA CAGGACTCAT CAAGCATACC GAGTATATGG AATTTTTAAA AAGCGTTCCA 
ACATTCCAGA GCCTTCCTGA AGAGATCCTC AGCAAGCTTG CTGATGTCCT TGAAGAGACC 
CACTATGAAA ATGGAGAATA TATTATCAGG CAAGGTGCAA GAGGGGACAC CTTCTTTATC 
ATCAGCAAAG GAACGGTAAA TGTCACTCGT GAAGACTCAC CGAGTGAAGA CCCAGTCTTT 
CTTAGAACTT TAGGAAAAGG AGACTGGTTT GGAGAGAAAG CCTTGCAGGG GGAAGATGTG 
AGAACAGCAA ACGTAATTGC TGCAGAAGCT GTAACCTGCC TTGTGATTGA CAGAGACTCT 
TTTAAACATT TGATTGGAGG GCTGGATGAT GTTTCTAATA AAGCATATGA AGATGCAGAA 
GCTAAAGCAA AATATGAAGC TGAAGCGGCT TTCTTCGCCA ACCTGAAGCT GTCTGATTTC 
AACATCATTG ATACCCTTGG AGTTGGAGGT TTCGGACGAG TAGAACTGGT CCAGTTGAAA 
AGTGAAGAAT CCAAAACGTT TGCAATGAAG ATTCTCAAGA AACGTCACAT TGTGGACACA 
AGACAGCAGG AGCACATCCG CTCAGAGAAG CAGATCATGC AGGGGGCTCA TTCCGATTTC 
ATAGTGAGAC TGTACAGAAC ATTTAAGGAC AGCAAATATT TGTATATGTT GATGGAAGCT 
TGTCTAGGTG GAGAGCTCTG GACCATTCTC AGGGATAGAG GTTCGTTTGA AGATTCTACA 
ACCAGATTTT ACACAGCATG TGTGGTAGAA GCTTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC AGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGATTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACTCCAGAGT ATGTAGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCAGCCGAC 
TACTGGTCAC TGGGAATCCT AATGTATGAA CTCCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA TAACATCATA TTGAGGGGGA TTGACATGAT AGAATTTCCA 
AAGAAGATTG CCAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA CAATCCATCA 
GAAAGATTAG GGAATTTGAA AAATGGAGTA AAAGACATTC AAAAGCACAA ATGGTTTGAG 
GGCTTTAACT GGGAAGGCTT AAGAAAAGGT ACCTTGACAC CTCCTATAAT ACCAAGTGTT 
GCATCACCCA CAGACACAAG TAATTTTGAC AGTTTCCCTG AGGACAACGA TGAACCACCA 
CCTGATGACA ACTCAGGATG GGATATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( XM_017016412.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871901.1 Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X1, mRNA.

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CloneID	OHu91517
Clone ID Related Accession (Same CDS sequence)	XM_017016412.1
Accession Version	XM_017016412.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATAGAC AATACTGTAG GCCAAGGTCC AAGGATCTTA TAAAGGAAGC TATCCTTGAC 
AATGACTTTA TGAAGAACTT GGAGCTGTCG CAGATCCAGG AGATTGTGGA TTGTATGTAC 
CCGGTGGAGT ATGGCAAGGA CAGTTGCATC ATCAAAGAAG GAGACGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGTAA GGTTGAAGTT ACAAAAGAAG GTGTGAAGTT GTGTACCATG 
GGTCCAGGAA AAGTGTTTGG GGAATTGGCT ATTCTTTACA ACTGTACCCG GACAGCGACC 
GTCAAGACTC TTGTAAATGT AAAACTCTGG GCCATTGATC GACAATGTTT TCAAACAATA 
ATGATGAGGA CAGGACTCAT CAAGCATACC GAGTATATGG AATTTTTAAA AAGCGTTCCA 
ACATTCCAGA GCCTTCCTGA AGAGATCCTC AGCAAGCTTG CTGATGTCCT TGAAGAGACC 
CACTATGAAA ATGGAGAATA TATTATCAGG CAAGGTGCAA GAGGGGACAC CTTCTTTATC 
ATCAGCAAAG GAACGGTAAA TGTCACTCGT GAAGACTCAC CGAGTGAAGA CCCAGTCTTT 
CTTAGAACTT TAGGAAAAGG AGACTGGTTT GGAGAGAAAG CCTTGCAGGG GGAAGATGTG 
AGAACAGCAA ACGTAATTGC TGCAGAAGCT GTAACCTGCC TTGTGATTGA CAGAGACTCT 
TTTAAACATT TGATTGGAGG GCTGGATGAT GTTTCTAATA AAGCATATGA AGATGCAGAA 
GCTAAAGCAA AATATGAAGC TGAAGCGGCT TTCTTCGCCA ACCTGAAGCT GTCTGATTTC 
AACATCATTG ATACCCTTGG AGTTGGAGGT TTCGGACGAG TAGAACTGGT CCAGTTGAAA 
AGTGAAGAAT CCAAAACGTT TGCAATGAAG ATTCTCAAGA AACGTCACAT TGTGGACACA 
AGACAGCAGG AGCACATCCG CTCAGAGAAG CAGATCATGC AGGGGGCTCA TTCCGATTTC 
ATAGTGAGAC TGTACAGAAC ATTTAAGGAC AGCAAATATT TGTATATGTT GATGGAAGCT 
TGTCTAGGTG GAGAGCTCTG GACCATTCTC AGGGATAGAG GTTCGTTTGA AGATTCTACA 
ACCAGATTTT ACACAGCATG TGTGGTAGAA GCTTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC AGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGATTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACTCCAGAGT ATGTAGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCAGCCGAC 
TACTGGTCAC TGGGAATCCT AATGTATGAA CTCCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA TAACATCATA TTGAGGGGGA TTGACATGAT AGAATTTCCA 
AAGAAGATTG CCAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA CAATCCATCA 
GAAAGATTAG GGAATTTGAA AAATGGAGTA AAAGACATTC AAAAGCACAA ATGGTTTGAG 
GGCTTTAACT GGGAAGGCTT AAGAAAAGGT ACCTTGACAC CTCCTATAAT ACCAAGTGTT 
GCATCACCCA CAGACACAAG TAATTTTGAC AGTTTCCCTG AGGACAACGA TGAACCACCA 
CCTGATGACA ACTCAGGATG GGATATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871901.1
CDS	955..2730
Translation	MDRQYCRPRSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	XM_017016412.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016412.1

ORF Insert Sequence:

ATGGATAGAC AATACTGTAG GCCAAGGTCC AAGGATCTTA TAAAGGAAGC TATCCTTGAC 
AATGACTTTA TGAAGAACTT GGAGCTGTCG CAGATCCAGG AGATTGTGGA TTGTATGTAC 
CCGGTGGAGT ATGGCAAGGA CAGTTGCATC ATCAAAGAAG GAGACGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGTAA GGTTGAAGTT ACAAAAGAAG GTGTGAAGTT GTGTACCATG 
GGTCCAGGAA AAGTGTTTGG GGAATTGGCT ATTCTTTACA ACTGTACCCG GACAGCGACC 
GTCAAGACTC TTGTAAATGT AAAACTCTGG GCCATTGATC GACAATGTTT TCAAACAATA 
ATGATGAGGA CAGGACTCAT CAAGCATACC GAGTATATGG AATTTTTAAA AAGCGTTCCA 
ACATTCCAGA GCCTTCCTGA AGAGATCCTC AGCAAGCTTG CTGATGTCCT TGAAGAGACC 
CACTATGAAA ATGGAGAATA TATTATCAGG CAAGGTGCAA GAGGGGACAC CTTCTTTATC 
ATCAGCAAAG GAACGGTAAA TGTCACTCGT GAAGACTCAC CGAGTGAAGA CCCAGTCTTT 
CTTAGAACTT TAGGAAAAGG AGACTGGTTT GGAGAGAAAG CCTTGCAGGG GGAAGATGTG 
AGAACAGCAA ACGTAATTGC TGCAGAAGCT GTAACCTGCC TTGTGATTGA CAGAGACTCT 
TTTAAACATT TGATTGGAGG GCTGGATGAT GTTTCTAATA AAGCATATGA AGATGCAGAA 
GCTAAAGCAA AATATGAAGC TGAAGCGGCT TTCTTCGCCA ACCTGAAGCT GTCTGATTTC 
AACATCATTG ATACCCTTGG AGTTGGAGGT TTCGGACGAG TAGAACTGGT CCAGTTGAAA 
AGTGAAGAAT CCAAAACGTT TGCAATGAAG ATTCTCAAGA AACGTCACAT TGTGGACACA 
AGACAGCAGG AGCACATCCG CTCAGAGAAG CAGATCATGC AGGGGGCTCA TTCCGATTTC 
ATAGTGAGAC TGTACAGAAC ATTTAAGGAC AGCAAATATT TGTATATGTT GATGGAAGCT 
TGTCTAGGTG GAGAGCTCTG GACCATTCTC AGGGATAGAG GTTCGTTTGA AGATTCTACA 
ACCAGATTTT ACACAGCATG TGTGGTAGAA GCTTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC AGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGATTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACTCCAGAGT ATGTAGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCAGCCGAC 
TACTGGTCAC TGGGAATCCT AATGTATGAA CTCCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA TAACATCATA TTGAGGGGGA TTGACATGAT AGAATTTCCA 
AAGAAGATTG CCAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA CAATCCATCA 
GAAAGATTAG GGAATTTGAA AAATGGAGTA AAAGACATTC AAAAGCACAA ATGGTTTGAG 
GGCTTTAACT GGGAAGGCTT AAGAAAAGGT ACCTTGACAC CTCCTATAAT ACCAAGTGTT 
GCATCACCCA CAGACACAAG TAATTTTGAC AGTTTCCCTG AGGACAACGA TGAACCACCA 
CCTGATGACA ACTCAGGATG GGATATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( XM_017016413.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016871902.1 Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X2, mRNA.

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CloneID	OHu91518
Clone ID Related Accession (Same CDS sequence)	XM_017016413.1
Accession Version	XM_017016413.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cGMP-dependent protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAGGT CCAAGGATCT TATAAAGGAA GCTATCCTTG ACAATGACTT TATGAAGAAC 
TTGGAGCTGT CGCAGATCCA GGAGATTGTG GATTGTATGT ACCCGGTGGA GTATGGCAAG 
GACAGTTGCA TCATCAAAGA AGGAGACGTG GGGTCACTGG TGTATGTCAT GGAAGATGGT 
AAGGTTGAAG TTACAAAAGA AGGTGTGAAG TTGTGTACCA TGGGTCCAGG AAAAGTGTTT 
GGGGAATTGG CTATTCTTTA CAACTGTACC CGGACAGCGA CCGTCAAGAC TCTTGTAAAT 
GTAAAACTCT GGGCCATTGA TCGACAATGT TTTCAAACAA TAATGATGAG GACAGGACTC 
ATCAAGCATA CCGAGTATAT GGAATTTTTA AAAAGCGTTC CAACATTCCA GAGCCTTCCT 
GAAGAGATCC TCAGCAAGCT TGCTGATGTC CTTGAAGAGA CCCACTATGA AAATGGAGAA 
TATATTATCA GGCAAGGTGC AAGAGGGGAC ACCTTCTTTA TCATCAGCAA AGGAACGGTA 
AATGTCACTC GTGAAGACTC ACCGAGTGAA GACCCAGTCT TTCTTAGAAC TTTAGGAAAA 
GGAGACTGGT TTGGAGAGAA AGCCTTGCAG GGGGAAGATG TGAGAACAGC AAACGTAATT 
GCTGCAGAAG CTGTAACCTG CCTTGTGATT GACAGAGACT CTTTTAAACA TTTGATTGGA 
GGGCTGGATG ATGTTTCTAA TAAAGCATAT GAAGATGCAG AAGCTAAAGC AAAATATGAA 
GCTGAAGCGG CTTTCTTCGC CAACCTGAAG CTGTCTGATT TCAACATCAT TGATACCCTT 
GGAGTTGGAG GTTTCGGACG AGTAGAACTG GTCCAGTTGA AAAGTGAAGA ATCCAAAACG 
TTTGCAATGA AGATTCTCAA GAAACGTCAC ATTGTGGACA CAAGACAGCA GGAGCACATC 
CGCTCAGAGA AGCAGATCAT GCAGGGGGCT CATTCCGATT TCATAGTGAG ACTGTACAGA 
ACATTTAAGG ACAGCAAATA TTTGTATATG TTGATGGAAG CTTGTCTAGG TGGAGAGCTC 
TGGACCATTC TCAGGGATAG AGGTTCGTTT GAAGATTCTA CAACCAGATT TTACACAGCA 
TGTGTGGTAG AAGCTTTTGC CTATCTGCAT TCCAAAGGAA TCATTTACAG GGACCTCAAG 
CCAGAAAATC TCATCCTAGA TCACCGAGGT TATGCCAAAC TGGTTGATTT TGGCTTTGCA 
AAGAAAATAG GATTTGGAAA GAAAACATGG ACTTTTTGTG GGACTCCAGA GTATGTAGCC 
CCAGAGATCA TCCTGAACAA AGGCCATGAC ATTTCAGCCG ACTACTGGTC ACTGGGAATC 
CTAATGTATG AACTCCTGAC TGGCAGCCCA CCTTTCTCAG GCCCAGATCC TATGAAAACC 
TATAACATCA TATTGAGGGG GATTGACATG ATAGAATTTC CAAAGAAGAT TGCCAAAAAT 
GCTGCTAATT TAATTAAAAA ACTATGCAGG GACAATCCAT CAGAAAGATT AGGGAATTTG 
AAAAATGGAG TAAAAGACAT TCAAAAGCAC AAATGGTTTG AGGGCTTTAA CTGGGAAGGC 
TTAAGAAAAG GTACCTTGAC ACCTCCTATA ATACCAAGTG TTGCATCACC CACAGACACA 
AGTAATTTTG ACAGTTTCCC TGAGGACAAC GATGAACCAC CACCTGATGA CAACTCAGGA 
TGGGATATAG ACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016871902.1
CDS	180..1937
Translation	MSRSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGTVNVTREDSPSEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	XM_017016413.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase cGMP-dependent 1 (PRKG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016413.1

ORF Insert Sequence:

ATGTCAAGGT CCAAGGATCT TATAAAGGAA GCTATCCTTG ACAATGACTT TATGAAGAAC 
TTGGAGCTGT CGCAGATCCA GGAGATTGTG GATTGTATGT ACCCGGTGGA GTATGGCAAG 
GACAGTTGCA TCATCAAAGA AGGAGACGTG GGGTCACTGG TGTATGTCAT GGAAGATGGT 
AAGGTTGAAG TTACAAAAGA AGGTGTGAAG TTGTGTACCA TGGGTCCAGG AAAAGTGTTT 
GGGGAATTGG CTATTCTTTA CAACTGTACC CGGACAGCGA CCGTCAAGAC TCTTGTAAAT 
GTAAAACTCT GGGCCATTGA TCGACAATGT TTTCAAACAA TAATGATGAG GACAGGACTC 
ATCAAGCATA CCGAGTATAT GGAATTTTTA AAAAGCGTTC CAACATTCCA GAGCCTTCCT 
GAAGAGATCC TCAGCAAGCT TGCTGATGTC CTTGAAGAGA CCCACTATGA AAATGGAGAA 
TATATTATCA GGCAAGGTGC AAGAGGGGAC ACCTTCTTTA TCATCAGCAA AGGAACGGTA 
AATGTCACTC GTGAAGACTC ACCGAGTGAA GACCCAGTCT TTCTTAGAAC TTTAGGAAAA 
GGAGACTGGT TTGGAGAGAA AGCCTTGCAG GGGGAAGATG TGAGAACAGC AAACGTAATT 
GCTGCAGAAG CTGTAACCTG CCTTGTGATT GACAGAGACT CTTTTAAACA TTTGATTGGA 
GGGCTGGATG ATGTTTCTAA TAAAGCATAT GAAGATGCAG AAGCTAAAGC AAAATATGAA 
GCTGAAGCGG CTTTCTTCGC CAACCTGAAG CTGTCTGATT TCAACATCAT TGATACCCTT 
GGAGTTGGAG GTTTCGGACG AGTAGAACTG GTCCAGTTGA AAAGTGAAGA ATCCAAAACG 
TTTGCAATGA AGATTCTCAA GAAACGTCAC ATTGTGGACA CAAGACAGCA GGAGCACATC 
CGCTCAGAGA AGCAGATCAT GCAGGGGGCT CATTCCGATT TCATAGTGAG ACTGTACAGA 
ACATTTAAGG ACAGCAAATA TTTGTATATG TTGATGGAAG CTTGTCTAGG TGGAGAGCTC 
TGGACCATTC TCAGGGATAG AGGTTCGTTT GAAGATTCTA CAACCAGATT TTACACAGCA 
TGTGTGGTAG AAGCTTTTGC CTATCTGCAT TCCAAAGGAA TCATTTACAG GGACCTCAAG 
CCAGAAAATC TCATCCTAGA TCACCGAGGT TATGCCAAAC TGGTTGATTT TGGCTTTGCA 
AAGAAAATAG GATTTGGAAA GAAAACATGG ACTTTTTGTG GGACTCCAGA GTATGTAGCC 
CCAGAGATCA TCCTGAACAA AGGCCATGAC ATTTCAGCCG ACTACTGGTC ACTGGGAATC 
CTAATGTATG AACTCCTGAC TGGCAGCCCA CCTTTCTCAG GCCCAGATCC TATGAAAACC 
TATAACATCA TATTGAGGGG GATTGACATG ATAGAATTTC CAAAGAAGAT TGCCAAAAAT 
GCTGCTAATT TAATTAAAAA ACTATGCAGG GACAATCCAT CAGAAAGATT AGGGAATTTG 
AAAAATGGAG TAAAAGACAT TCAAAAGCAC AAATGGTTTG AGGGCTTTAA CTGGGAAGGC 
TTAAGAAAAG GTACCTTGAC ACCTCCTATA ATACCAAGTG TTGCATCACC CACAGACACA 
AGTAATTTTG ACAGTTTCCC TGAGGACAAC GATGAACCAC CACCTGATGA CAACTCAGGA 
TGGGATATAG ACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PRKG1
Entrez Gene ID	5592
Full Name	protein kinase, cGMP-dependent, type I
Synonyms	AAT8,PKG,PRKG1B,PRKGR1B,cGK,cGK 1,cGK1,cGKI,cGKI-BETA,cGKI-alpha
General protein information	Preferred Names protein kinase, cGMP-dependent, type I Names cGMP-dependent protein kinase 1 protein kinase, cGMP-dependent, regulatory, type I, beta
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q11.23-q21.1
Summary	Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011].
Disorder MIM:	176894
Disorder Html:	Aortic aneurysm, familial thoracic 8, 615436 (3)

Homo sapiens (human)	PRKG1	NP_006249.1
Mus musculus (house mouse)	Prkg1	NP_001013855.1
Gallus gallus (chicken)	PRKG1	XP_003641507.2
	PRKG1	XP_001099460.1
Rattus norvegicus (Norway rat)	Prkg1	NP_001099201.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008863	XP_319605.4
Canis lupus familiaris (dog)	PRKG1	XP_851997.2
Bos taurus (cattle)	PRKG1	NP_776861.1
Danio rerio (zebrafish)	prkg1a	NP_957324.1
Drosophila melanogaster (fruit fly)	for	NP_477488.1
Xenopus tropicalis (tropical clawed frog)	LOC100494278	XP_002935474.2
Pan troglodytes (chimpanzee)	PRKG1	XP_001162858.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PRKG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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