ASF1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASF1B
Entrez Gene ID	55723
Full Name	anti-silencing function 1B histone chaperone
Synonyms	CIA-II
General protein information	Preferred Names anti-silencing function 1B histone chaperone Names histone chaperone ASF1B ASF1 anti-silencing function 1 homolog B CCG1-interacting factor A-II anti-silencing function protein 1 homolog B hAsf1 hAsf1b hCIA-II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.12
Summary	This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008].
Disorder MIM:	609190

mRNA and Protein(s)

mRNA	Protein	Name
NM_018154.3	NP_060624.1	histone chaperone ASF1B
NM_018154.2	NP_060624.1	histone chaperone ASF1B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ASF1B	XP_001171489.1
	LOC717646	XP_001111289.1
Mus musculus (house mouse)	Asf1b	NP_077146.1
Magnaporthe oryzae (rice blast fungus)	MGG_14874	XP_003716703.1
Homo sapiens (human)	ASF1B	NP_060624.1
Canis lupus familiaris (dog)	ASF1B	XP_542021.1
Bos taurus (cattle)	ASF1B	NP_001068921.1
Rattus norvegicus (Norway rat)	Asf1b	NP_001100630.1
Neurospora crassa	NCU09436	XP_958588.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Histone chaperone ASF1B promotes human ?-cell proliferation via recruitment of histone H3.3.
Paul PK, Rabaglia ME, Wang CY, Stapleton DS, Leng N, Kendziorski C, Lewis PW, Keller MP, Attie AD
Cell cycle (Georgetown, Tex.)15(23)3191-3202(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

histone binding to ASF1B is required for the induction of beta-cell proliferation.
Title: Histone chaperone ASF1B promotes human ?-cell proliferation via recruitment of histone H3.3.

Data indicate Tousled-like kinases (TLK1) phosphorylation has an impact on cell cycle proteins Asf1a and Asf1b function.
Title: Tousled-like kinases phosphorylate Asf1 to promote histone supply during DNA replication.

Co-depletion of the histone chaperones ASF1a and ASF1b in human cells induces all hallmarks of alternative lengthening of telomeres in both primary and cancer cells.
Title: Rapid induction of alternative lengthening of telomeres by depletion of the histone chaperone ASF1.

Data indicate that miR-214 down-regulated the expression of PSMD10 (gankyrin) and ASF1B, and gankyrin inhibition induced an increase of P53 mRNA levels.
Title: Restoration of microRNA-214 expression reduces growth of myeloma cells through positive regulation of P53 and inhibition of DNA replication.

study of the interaction of the histone H3-H4 complex with the RbAp48 and their exchange with a second histone chaperone, anti-silencing function protein 1 (ASF1); exchange of histones H3-H4 between these two histone chaperones has a central role in the assembly of new nucleosomes
Title: Structural plasticity of histones H3-H4 facilitates their allosteric exchange between RbAp48 and ASF1.

The following ASF1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASF1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16495	NM_018154.3 Latest version!	Homo sapiens anti-silencing function 1B histone chaperone (ASF1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu16495	NM_018154.2	Homo sapiens anti-silencing function 1B histone chaperone (ASF1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASF1B ( NM_018154.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060624.1 Homo sapiens anti-silencing function 1B histone chaperone (ASF1B), mRNA.

Price & Availability↑

CloneID	OHu16495
Clone ID Related Accession (Same CDS sequence)	NM_018154.3 , NM_018154.2
Accession Version	NM_018154.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	histone chaperone ASF1B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK001466.1 and BC036521.1. On Nov 22, 2018 this sequence version replaced NM_018154.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.93938.1, BC036521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263382.8/ ENSP00000263382.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAAGG TGTCGGTGCT GAACGTGGCG GTCCTGGAGA ACCCGAGCCC TTTCCACAGC 
CCCTTCCGGT TCGAGATCAG CTTCGAGTGC AGTGAAGCCC TGGCGGACGA CCTGGAGTGG 
AAGATCATTT ATGTTGGCTC GGCTGAGAGT GAGGAATTTG ATCAGATCCT AGACTCGGTG 
CTGGTGGGCC CTGTGCCAGC AGGGAGACAC ATGTTTGTCT TTCAGGCCGA CGCCCCCAAC 
CCATCCCTCA TCCCAGAGAC TGATGCCGTG GGTGTGACTG TGGTCCTCAT CACCTGCACC 
TACCATGGAC AGGAGTTCAT CCGAGTGGGC TACTACGTCA ACAACGAGTA CCTCAACCCT 
GAGCTGCGTG AGAACCCGCC CATGAAGCCA GATTTCTCCC AGCTCCAGCG GAACATCTTG 
GCCTCGAACC CCCGGGTGAC CCGCTTCCAT ATCAACTGGG ACAACAACAT GGACAGGCTG 
GAGGCCATAG AGACCCAGGA CCCCTCCCTG GGCTGCGGCC TCCCACTCAA CTGCACTCCT 
ATCAAGGGCT TGGGGCTCCC TGGCTGCATC CCTGGCCTCC TCCCTGAGAA CTCCATGGAC 
TGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060624.1
CDS	134..742
Translation	MAKVSVLNVAVLENPSPFHSPFRFEISFECSEALADDLEWKIIYVGSAESEEFDQILDSVLVGPVPAGRHMFVFQADAPNPSLIPETDAVGVTVVLITCTYHGQEFIRVGYYVNNEYLNPELRENPPMKPDFSQLQRNILASNPRVTRFHINWDNNMDRLEAIETQDPSLGCGLPLNCTPIKGLGLPGCIPGLLPENSMDCI

Target ORF information:

RefSeq Version	NM_018154.3
Organism	Homo sapiens(human)
Definition	Homo sapiens anti-silencing function 1B histone chaperone (ASF1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018154.3

ORF Insert Sequence:

ATGGCCAAGG TGTCGGTGCT GAACGTGGCG GTCCTGGAGA ACCCGAGCCC TTTCCACAGC 
CCCTTCCGGT TCGAGATCAG CTTCGAGTGC AGTGAAGCCC TGGCGGACGA CCTGGAGTGG 
AAGATCATTT ATGTTGGCTC GGCTGAGAGT GAGGAATTTG ATCAGATCCT AGACTCGGTG 
CTGGTGGGCC CTGTGCCAGC AGGGAGACAC ATGTTTGTCT TTCAGGCCGA CGCCCCCAAC 
CCATCCCTCA TCCCAGAGAC TGATGCCGTG GGTGTGACTG TGGTCCTCAT CACCTGCACC 
TACCATGGAC AGGAGTTCAT CCGAGTGGGC TACTACGTCA ACAACGAGTA CCTCAACCCT 
GAGCTGCGTG AGAACCCGCC CATGAAGCCA GATTTCTCCC AGCTCCAGCG GAACATCTTG 
GCCTCGAACC CCCGGGTGAC CCGCTTCCAT ATCAACTGGG ACAACAACAT GGACAGGCTG 
GAGGCCATAG AGACCCAGGA CCCCTCCCTG GGCTGCGGCC TCCCACTCAA CTGCACTCCT 
ATCAAGGGCT TGGGGCTCCC TGGCTGCATC CCTGGCCTCC TCCCTGAGAA CTCCATGGAC 
TGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASF1B ( NM_018154.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_060624.1 Homo sapiens anti-silencing function 1B histone chaperone (ASF1B), mRNA.

Price & Availability↑

CloneID	OHu16495
Clone ID Related Accession (Same CDS sequence)	NM_018154.3 , NM_018154.2
Accession Version	NM_018154.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 609bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-28
Organism	Homo sapiens(human)
Product	histone chaperone ASF1B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN426445.1, AK001466.1 and BC036521.1. On Jun 15, 2005 this sequence version replaced NM_018154.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC036521.1, SRR1163658.173006.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG TGTCGGTGCT GAACGTGGCG GTCCTGGAGA ACCCGAGCCC TTTCCACAGC 
CCCTTCCGGT TCGAGATCAG CTTCGAGTGC AGTGAAGCCC TGGCGGACGA CCTGGAGTGG 
AAGATCATTT ATGTTGGCTC GGCTGAGAGT GAGGAATTTG ATCAGATCCT AGACTCGGTG 
CTGGTGGGCC CTGTGCCAGC AGGGAGACAC ATGTTTGTCT TTCAGGCCGA CGCCCCCAAC 
CCATCCCTCA TCCCAGAGAC TGATGCCGTG GGTGTGACTG TGGTCCTCAT CACCTGCACC 
TACCATGGAC AGGAGTTCAT CCGAGTGGGC TACTACGTCA ACAACGAGTA CCTCAACCCT 
GAGCTGCGTG AGAACCCGCC CATGAAGCCA GATTTCTCCC AGCTCCAGCG GAACATCTTG 
GCCTCGAACC CCCGGGTGAC CCGCTTCCAT ATCAACTGGG ACAACAACAT GGACAGGCTG 
GAGGCCATAG AGACCCAGGA CCCCTCCCTG GGCTGCGGCC TCCCACTCAA CTGCACTCCT 
ATCAAGGGCT TGGGGCTCCC TGGCTGCATC CCTGGCCTCC TCCCTGAGAA CTCCATGGAC 
TGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060624.1
CDS	173..781
Translation	MAKVSVLNVAVLENPSPFHSPFRFEISFECSEALADDLEWKIIYVGSAESEEFDQILDSVLVGPVPAGRHMFVFQADAPNPSLIPETDAVGVTVVLITCTYHGQEFIRVGYYVNNEYLNPELRENPPMKPDFSQLQRNILASNPRVTRFHINWDNNMDRLEAIETQDPSLGCGLPLNCTPIKGLGLPGCIPGLLPENSMDCI

Target ORF information:

RefSeq Version	NM_018154.2
Organism	Homo sapiens(human)
Definition	Homo sapiens anti-silencing function 1B histone chaperone (ASF1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018154.2

ORF Insert Sequence:

ATGGCCAAGG TGTCGGTGCT GAACGTGGCG GTCCTGGAGA ACCCGAGCCC TTTCCACAGC 
CCCTTCCGGT TCGAGATCAG CTTCGAGTGC AGTGAAGCCC TGGCGGACGA CCTGGAGTGG 
AAGATCATTT ATGTTGGCTC GGCTGAGAGT GAGGAATTTG ATCAGATCCT AGACTCGGTG 
CTGGTGGGCC CTGTGCCAGC AGGGAGACAC ATGTTTGTCT TTCAGGCCGA CGCCCCCAAC 
CCATCCCTCA TCCCAGAGAC TGATGCCGTG GGTGTGACTG TGGTCCTCAT CACCTGCACC 
TACCATGGAC AGGAGTTCAT CCGAGTGGGC TACTACGTCA ACAACGAGTA CCTCAACCCT 
GAGCTGCGTG AGAACCCGCC CATGAAGCCA GATTTCTCCC AGCTCCAGCG GAACATCTTG 
GCCTCGAACC CCCGGGTGAC CCGCTTCCAT ATCAACTGGG ACAACAACAT GGACAGGCTG 
GAGGCCATAG AGACCCAGGA CCCCTCCCTG GGCTGCGGCC TCCCACTCAA CTGCACTCCT 
ATCAAGGGCT TGGGGCTCCC TGGCTGCATC CCTGGCCTCC TCCCTGAGAA CTCCATGGAC 
TGCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Histone chaperone ASF1B promotes human ?-cell proliferation via recruitment of histone H3.3.
Cell cycle (Georgetown, Tex.)15(23)3191-3202(2016 Dec)
Paul PK,Rabaglia ME,Wang CY,Stapleton DS,Leng N,Kendziorski C,Lewis PW,Keller MP,Attie AD

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ASF1B cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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