KIRREL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KIRREL1
Entrez Gene ID	55243
Full Name	kirre like nephrin family adhesion molecule 1
Synonyms	KIRREL,NEPH1
General protein information	Preferred Names kirre like nephrin family adhesion molecule 1 Names kin of IRRE-like protein 1 kin of IRRE like kin of irregular chiasm-like protein 1 nephrin related nephrin-like protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q23.1
Summary	NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2; MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM, Mar 2008].
Disorder MIM:	607428

mRNA and Protein(s)

mRNA	Protein	Name
XM_005245305.5	XP_005245362.1	kin of IRRE-like protein 1 isoform X1
NM_018240.7	NP_060710.3	kin of IRRE-like protein 1 isoform 1 precursor
XM_005245305.4	XP_005245362.1	kin of IRRE-like protein 1 isoform X1
NM_018240.6	NP_060710.3	kin of IRRE-like protein 1 isoform 1 precursor
NM_001286349.1	NP_001273278.1	kin of IRRE-like protein 1 isoform 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KIRREL	XP_005640973.1
	KIRREL	XP_001116954.1
Danio rerio (zebrafish)	kirrelb	NP_001124123.1
Pan troglodytes (chimpanzee)	KIRREL	XP_003308572.1
Bos taurus (cattle)	KIRREL	XP_003585870.2
Rattus norvegicus (Norway rat)	Kirrel	NP_997489.1
Danio rerio (zebrafish)	kirrela	NP_998033.1
Xenopus tropicalis (tropical clawed frog)	kirrel	XP_002938994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000218	XP_310916.5
Homo sapiens (human)	KIRREL	NP_060710.3
Mus musculus (house mouse)	Kirrel	NP_570937.2
Gallus gallus (chicken)	KIRREL	XP_004948232.1
Drosophila melanogaster (fruit fly)	rst	NP_525058.2

Gene Ontology
Bibliography

Related articles in PubMed

Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1.
Arif E, Sharma P, Solanki A, Mallik L, Rathore YS, Twal WO, Nath SK, Gandhi D, Holzman LB, Ostap EM, Ashish, Nihalani D
Molecular and cellular biology36(11)1639-54(2016 06)

Slit diaphragm protein Neph1 and its signaling: a novel therapeutic target for protection of podocytes against glomerular injury.
Arif E, Rathore YS, Kumari B, Ashish F, Wong HN, Holzman LB, Nihalani D
The Journal of biological chemistry289(14)9502-18(2014 Apr)

Solution structure analysis of cytoplasmic domain of podocyte protein Neph1 using small/wide angle x-ray scattering (SWAXS).
Mallik L, Arif E, Sharma P, Rathore YS, Wong HN, Holzman LB, Ashish, Nihalani D
The Journal of biological chemistry287(12)9441-53(2012 Mar)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

Motor protein Myo1c is a podocyte protein that facilitates the transport of slit diaphragm protein Neph1 to the podocyte membrane.
Arif E, Wagner MC, Johnstone DB, Wong HN, George B, Pruthi PA, Lazzara MJ, Nihalani D
Molecular and cellular biology31(10)2134-50(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Analysis of interaction surfaces between NEPH1 and MYO1c led to the identification of a critical residue in Neph1 involved in binding to Myo1c. Indeed, a point mutant from this site abolished interaction between Neph1 and Myo1c.
Title: Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1.

maintaining high levels of Neph1 at the membrane using a podocyte cell line overexpressing chimeric Neph1 increased the ability of podocytes to resist PAN-induced injury and PAN-induced albumin leakage
Title: Slit diaphragm protein Neph1 and its signaling: a novel therapeutic target for protection of podocytes against glomerular injury.

Neph1-CD adopts a global shape in solution, and its interaction with ZO-1 involves multiple sites.
Title: Solution structure analysis of cytoplasmic domain of podocyte protein Neph1 using small/wide angle x-ray scattering (SWAXS).

Localization of Neph1 to the podocyte cell membrane is altered in the presence of mutant Myo1c, and is actin dependent. Knockdown of Myo1c inhibits Neph1 membrane localization. Neph1 is critical for the maintenance of glomerular function.
Title: Motor protein Myo1c is a podocyte protein that facilitates the transport of slit diaphragm protein Neph1 to the podocyte membrane.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genotype-phenotype correlations in non-Finnish congenital nephrotic syndrome.

The following KIRREL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KIRREL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11848	NM_018240.7 Latest version!	Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu10967	NM_001286349.1 Latest version!	Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu34525	XM_005245305.5 Latest version!	Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu34525	XM_005245305.4	Homo sapiens kin of IRRE like (Drosophila) (KIRREL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu11848	NM_018240.6	Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KIRREL1 ( NM_018240.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_060710.3 Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11848
Clone ID Related Accession (Same CDS sequence)	NM_018240.7 , NM_018240.6
Accession Version	NM_018240.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	kin of IRRE-like protein 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC109192.1, AL139010.29 and AA628934.1. On Nov 23, 2018 this sequence version replaced NM_018240.6. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359209.11/ ENSP00000352138.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGGGACCCAG 
ACCCGCTTCA GCCAGGAGCC AGCTGACCAG ACGGTGGTGG CTGGACAGCG GGCCGTGCTC 
CCCTGTGTGC TGCTCAACTA CTCTGGAATT GTGCAATGGA CCAAGGACGG GCTGGCCCTG 
GGCATGGGCC AGGGCCTCAA AGCCTGGCCA CGGTACCGGG TTGTGGGCTC CGCAGACGCT 
GGGCAGTACA ACCTGGAGAT CACAGATGCT GAGCTCTCTG ACGACGCCTC TTACGAGTGC 
CAGGCCACGG AGGCCGCCCT GCGCTCTCGG CGGGCCAAAC TCACCGTGCT CATCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGTCCTG AGTAACAGCA ACCAGCTGCT GCTGAAGTCG 
GTGACTCAGG CAGACGCTGG CACCTACACC TGCCGGGCCA TCGTGCCTCG AATCGGAGTG 
GCTGAGCGGG AGGTGCCGCT CTATGTGAAC GGGCCCCCCA TCATCTCCAG TGAGGCAGTG 
CAGTATGCTG TGAGGGGTGA CGGTGGCAAG GTGGAGTGTT TCATTGGGAG CACACCACCC 
CCAGACCGCA TAGCATGGGC CTGGAAGGAG AACTTCTTGG AGGTGGGGAC CCTGGAACGC 
TATACAGTGG AGAGGACCAA CTCAGGCAGT GGGGTGCTAT CCACGCTCAC CATCAACAAT 
GTCATGGAGG CCGACTTTCA GACTCACTAC AACTGCACCG CCTGGAACAG CTTCGGGCCA 
GGCACAGCCA TCATCCAGCT GGAAGAGCGA GAGGTGTTAC CTGTGGGCAT CATAGCTGGG 
GCCACCATCG GCGCGAGCAT CCTGCTCATC TTCTTCTTCA TCGCCTTGGT ATTCTTCCTC 
TACCGGCGCC GCAAAGGCAG TCGCAAAGAC GTGACCCTGA GGAAGCTGGA TATCAAGGTG 
GAGACAGTGA ACCGAGAGCC ACTTACGATG CATTCTGACC GGGAGGATGA CACCGCCAGC 
GTCTCCACAG CAACCCGGGT CATGAAGGCC ATCTACTCGT CGTTTAAGGA TGATGTGGAT 
CTGAAGCAGG ACCTGCGCTG CGACACCATC GACACCCGGG AGGAGTATGA GATGAAGGAC 
CCCACCAATG GCTACTACAA CGTGCGTGCC CATGAAGACC GCCCGTCTTC CAGGGCAGTG 
CTCTATGCTG ACTACCGTGC CCCTGGCCCT GCCCGCTTCG ACGGCCGCCC CTCATCCCGT 
CTCTCCCACT CCAGCGGCTA TGCCCAGCTC AACACCTATA GCCGGGGCCC TGCCTCTGAC 
TATGGCCCTG AGCCCACACC CCCTGGCCCT GCTGCCCCAG CTGGCACTGA CACAACCAGC 
CAGCTGTCCT ACGAGAACTA TGAGAAGTTC AACTCCCATC CCTTCCCTGG GGCAGCTGGG 
TACCCCACCT ACCGACTGGG CTACCCCCAG GCCCCACCCT CTGGCCTGGA GCGGACCCCA 
TATGAGGCGT ATGACCCCAT TGGCAAGTAC GCCACAGCCA CTCGATTCTC CTACACCTCC 
CAGCACTCGG ACTACGGCCA GCGATTCCAG CAGCGCATGC AGACTCACGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060710.3
CDS	33..2306
Translation	MLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV

Target ORF information:

RefSeq Version	NM_018240.7
Organism	Homo sapiens(human)
Definition	Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018240.7

ORF Insert Sequence:

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGGGACCCAG 
ACCCGCTTCA GCCAGGAGCC AGCTGACCAG ACGGTGGTGG CTGGACAGCG GGCCGTGCTC 
CCCTGTGTGC TGCTCAACTA CTCTGGAATT GTGCAATGGA CCAAGGACGG GCTGGCCCTG 
GGCATGGGCC AGGGCCTCAA AGCCTGGCCA CGGTACCGGG TTGTGGGCTC CGCAGACGCT 
GGGCAGTACA ACCTGGAGAT CACAGATGCT GAGCTCTCTG ACGACGCCTC TTACGAGTGC 
CAGGCCACGG AGGCCGCCCT GCGCTCTCGG CGGGCCAAAC TCACCGTGCT CATCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGTCCTG AGTAACAGCA ACCAGCTGCT GCTGAAGTCG 
GTGACTCAGG CAGACGCTGG CACCTACACC TGCCGGGCCA TCGTGCCTCG AATCGGAGTG 
GCTGAGCGGG AGGTGCCGCT CTATGTGAAC GGGCCCCCCA TCATCTCCAG TGAGGCAGTG 
CAGTATGCTG TGAGGGGTGA CGGTGGCAAG GTGGAGTGTT TCATTGGGAG CACACCACCC 
CCAGACCGCA TAGCATGGGC CTGGAAGGAG AACTTCTTGG AGGTGGGGAC CCTGGAACGC 
TATACAGTGG AGAGGACCAA CTCAGGCAGT GGGGTGCTAT CCACGCTCAC CATCAACAAT 
GTCATGGAGG CCGACTTTCA GACTCACTAC AACTGCACCG CCTGGAACAG CTTCGGGCCA 
GGCACAGCCA TCATCCAGCT GGAAGAGCGA GAGGTGTTAC CTGTGGGCAT CATAGCTGGG 
GCCACCATCG GCGCGAGCAT CCTGCTCATC TTCTTCTTCA TCGCCTTGGT ATTCTTCCTC 
TACCGGCGCC GCAAAGGCAG TCGCAAAGAC GTGACCCTGA GGAAGCTGGA TATCAAGGTG 
GAGACAGTGA ACCGAGAGCC ACTTACGATG CATTCTGACC GGGAGGATGA CACCGCCAGC 
GTCTCCACAG CAACCCGGGT CATGAAGGCC ATCTACTCGT CGTTTAAGGA TGATGTGGAT 
CTGAAGCAGG ACCTGCGCTG CGACACCATC GACACCCGGG AGGAGTATGA GATGAAGGAC 
CCCACCAATG GCTACTACAA CGTGCGTGCC CATGAAGACC GCCCGTCTTC CAGGGCAGTG 
CTCTATGCTG ACTACCGTGC CCCTGGCCCT GCCCGCTTCG ACGGCCGCCC CTCATCCCGT 
CTCTCCCACT CCAGCGGCTA TGCCCAGCTC AACACCTATA GCCGGGGCCC TGCCTCTGAC 
TATGGCCCTG AGCCCACACC CCCTGGCCCT GCTGCCCCAG CTGGCACTGA CACAACCAGC 
CAGCTGTCCT ACGAGAACTA TGAGAAGTTC AACTCCCATC CCTTCCCTGG GGCAGCTGGG 
TACCCCACCT ACCGACTGGG CTACCCCCAG GCCCCACCCT CTGGCCTGGA GCGGACCCCA 
TATGAGGCGT ATGACCCCAT TGGCAAGTAC GCCACAGCCA CTCGATTCTC CTACACCTCC 
CAGCACTCGG ACTACGGCCA GCGATTCCAG CAGCGCATGC AGACTCACGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIRREL1 ( NM_018240.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060710.3 Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11848
Clone ID Related Accession (Same CDS sequence)	NM_018240.7 , NM_018240.6
Accession Version	NM_018240.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	kin of IRRE-like protein 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY302131.1, BC109192.1, AL139010.29 and AA628934.1. On Nov 2, 2013 this sequence version replaced NM_018240.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC109192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGGGACCCAG 
ACCCGCTTCA GCCAGGAGCC AGCTGACCAG ACGGTGGTGG CTGGACAGCG GGCCGTGCTC 
CCCTGTGTGC TGCTCAACTA CTCTGGAATT GTGCAATGGA CCAAGGACGG GCTGGCCCTG 
GGCATGGGCC AGGGCCTCAA AGCCTGGCCA CGGTACCGGG TTGTGGGCTC CGCAGACGCT 
GGGCAGTACA ACCTGGAGAT CACAGATGCT GAGCTCTCTG ACGACGCCTC TTACGAGTGC 
CAGGCCACGG AGGCCGCCCT GCGCTCTCGG CGGGCCAAAC TCACCGTGCT CATCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGTCCTG AGTAACAGCA ACCAGCTGCT GCTGAAGTCG 
GTGACTCAGG CAGACGCTGG CACCTACACC TGCCGGGCCA TCGTGCCTCG AATCGGAGTG 
GCTGAGCGGG AGGTGCCGCT CTATGTGAAC GGGCCCCCCA TCATCTCCAG TGAGGCAGTG 
CAGTATGCTG TGAGGGGTGA CGGTGGCAAG GTGGAGTGTT TCATTGGGAG CACACCACCC 
CCAGACCGCA TAGCATGGGC CTGGAAGGAG AACTTCTTGG AGGTGGGGAC CCTGGAACGC 
TATACAGTGG AGAGGACCAA CTCAGGCAGT GGGGTGCTAT CCACGCTCAC CATCAACAAT 
GTCATGGAGG CCGACTTTCA GACTCACTAC AACTGCACCG CCTGGAACAG CTTCGGGCCA 
GGCACAGCCA TCATCCAGCT GGAAGAGCGA GAGGTGTTAC CTGTGGGCAT CATAGCTGGG 
GCCACCATCG GCGCGAGCAT CCTGCTCATC TTCTTCTTCA TCGCCTTGGT ATTCTTCCTC 
TACCGGCGCC GCAAAGGCAG TCGCAAAGAC GTGACCCTGA GGAAGCTGGA TATCAAGGTG 
GAGACAGTGA ACCGAGAGCC ACTTACGATG CATTCTGACC GGGAGGATGA CACCGCCAGC 
GTCTCCACAG CAACCCGGGT CATGAAGGCC ATCTACTCGT CGTTTAAGGA TGATGTGGAT 
CTGAAGCAGG ACCTGCGCTG CGACACCATC GACACCCGGG AGGAGTATGA GATGAAGGAC 
CCCACCAATG GCTACTACAA CGTGCGTGCC CATGAAGACC GCCCGTCTTC CAGGGCAGTG 
CTCTATGCTG ACTACCGTGC CCCTGGCCCT GCCCGCTTCG ACGGCCGCCC CTCATCCCGT 
CTCTCCCACT CCAGCGGCTA TGCCCAGCTC AACACCTATA GCCGGGGCCC TGCCTCTGAC 
TATGGCCCTG AGCCCACACC CCCTGGCCCT GCTGCCCCAG CTGGCACTGA CACAACCAGC 
CAGCTGTCCT ACGAGAACTA TGAGAAGTTC AACTCCCATC CCTTCCCTGG GGCAGCTGGG 
TACCCCACCT ACCGACTGGG CTACCCCCAG GCCCCACCCT CTGGCCTGGA GCGGACCCCA 
TATGAGGCGT ATGACCCCAT TGGCAAGTAC GCCACAGCCA CTCGATTCTC CTACACCTCC 
CAGCACTCGG ACTACGGCCA GCGATTCCAG CAGCGCATGC AGACTCACGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060710.3
CDS	405..2678
Translation	MLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV

Target ORF information:

RefSeq Version	NM_018240.6
Organism	Homo sapiens(human)
Definition	Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018240.6

ORF Insert Sequence:

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGGGACCCAG 
ACCCGCTTCA GCCAGGAGCC AGCTGACCAG ACGGTGGTGG CTGGACAGCG GGCCGTGCTC 
CCCTGTGTGC TGCTCAACTA CTCTGGAATT GTGCAATGGA CCAAGGACGG GCTGGCCCTG 
GGCATGGGCC AGGGCCTCAA AGCCTGGCCA CGGTACCGGG TTGTGGGCTC CGCAGACGCT 
GGGCAGTACA ACCTGGAGAT CACAGATGCT GAGCTCTCTG ACGACGCCTC TTACGAGTGC 
CAGGCCACGG AGGCCGCCCT GCGCTCTCGG CGGGCCAAAC TCACCGTGCT CATCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGTCCTG AGTAACAGCA ACCAGCTGCT GCTGAAGTCG 
GTGACTCAGG CAGACGCTGG CACCTACACC TGCCGGGCCA TCGTGCCTCG AATCGGAGTG 
GCTGAGCGGG AGGTGCCGCT CTATGTGAAC GGGCCCCCCA TCATCTCCAG TGAGGCAGTG 
CAGTATGCTG TGAGGGGTGA CGGTGGCAAG GTGGAGTGTT TCATTGGGAG CACACCACCC 
CCAGACCGCA TAGCATGGGC CTGGAAGGAG AACTTCTTGG AGGTGGGGAC CCTGGAACGC 
TATACAGTGG AGAGGACCAA CTCAGGCAGT GGGGTGCTAT CCACGCTCAC CATCAACAAT 
GTCATGGAGG CCGACTTTCA GACTCACTAC AACTGCACCG CCTGGAACAG CTTCGGGCCA 
GGCACAGCCA TCATCCAGCT GGAAGAGCGA GAGGTGTTAC CTGTGGGCAT CATAGCTGGG 
GCCACCATCG GCGCGAGCAT CCTGCTCATC TTCTTCTTCA TCGCCTTGGT ATTCTTCCTC 
TACCGGCGCC GCAAAGGCAG TCGCAAAGAC GTGACCCTGA GGAAGCTGGA TATCAAGGTG 
GAGACAGTGA ACCGAGAGCC ACTTACGATG CATTCTGACC GGGAGGATGA CACCGCCAGC 
GTCTCCACAG CAACCCGGGT CATGAAGGCC ATCTACTCGT CGTTTAAGGA TGATGTGGAT 
CTGAAGCAGG ACCTGCGCTG CGACACCATC GACACCCGGG AGGAGTATGA GATGAAGGAC 
CCCACCAATG GCTACTACAA CGTGCGTGCC CATGAAGACC GCCCGTCTTC CAGGGCAGTG 
CTCTATGCTG ACTACCGTGC CCCTGGCCCT GCCCGCTTCG ACGGCCGCCC CTCATCCCGT 
CTCTCCCACT CCAGCGGCTA TGCCCAGCTC AACACCTATA GCCGGGGCCC TGCCTCTGAC 
TATGGCCCTG AGCCCACACC CCCTGGCCCT GCTGCCCCAG CTGGCACTGA CACAACCAGC 
CAGCTGTCCT ACGAGAACTA TGAGAAGTTC AACTCCCATC CCTTCCCTGG GGCAGCTGGG 
TACCCCACCT ACCGACTGGG CTACCCCCAG GCCCCACCCT CTGGCCTGGA GCGGACCCCA 
TATGAGGCGT ATGACCCCAT TGGCAAGTAC GCCACAGCCA CTCGATTCTC CTACACCTCC 
CAGCACTCGG ACTACGGCCA GCGATTCCAG CAGCGCATGC AGACTCACGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIRREL1 ( NM_001286349.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273278.1 Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 2, mRNA.

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CloneID	OHu10967
Clone ID Related Accession (Same CDS sequence)	NM_001286349.1
Accession Version	NM_001286349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	kin of IRRE-like protein 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY302131.1, AK297788.1, AL139010.29 and AA628934.1. Transcript Variant: This variant (2) lacks two in-frame exons in the 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297788.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END##

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGTCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGTCCTG AGTAACAGCA ACCAGCTGCT GCTGAAGTCG 
GTGACTCAGG CAGACGCTGG CACCTACACC TGCCGGGCCA TCGTGCCTCG AATCGGAGTG 
GCTGAGCGGG AGGTGCCGCT CTATGTGAAC GGGCCCCCCA TCATCTCCAG TGAGGCAGTG 
CAGTATGCTG TGAGGGGTGA CGGTGGCAAG GTGGAGTGTT TCATTGGGAG CACACCACCC 
CCAGACCGCA TAGCATGGGC CTGGAAGGAG AACTTCTTGG AGGTGGGGAC CCTGGAACGC 
TATACAGTGG AGAGGACCAA CTCAGGCAGT GGGGTGCTAT CCACGCTCAC CATCAACAAT 
GTCATGGAGG CCGACTTTCA GACTCACTAC AACTGCACCG CCTGGAACAG CTTCGGGCCA 
GGCACAGCCA TCATCCAGCT GGAAGAGCGA GAGGTGTTAC CTGTGGGCAT CATAGCTGGG 
GCCACCATCG GCGCGAGCAT CCTGCTCATC TTCTTCTTCA TCGCCTTGGT ATTCTTCCTC 
TACCGGCGCC GCAAAGGCAG TCGCAAAGAC GTGACCCTGA GGAAGCTGGA TATCAAGGTG 
GAGACAGTGA ACCGAGAGCC ACTTACGATG CATTCTGACC GGGAGGATGA CACCGCCAGC 
GTCTCCACAG CAACCCGGGT CATGAAGGCC ATCTACTCGT CGTTTAAGGA TGATGTGGAT 
CTGAAGCAGG ACCTGCGCTG CGACACCATC GACACCCGGG AGGAGTATGA GATGAAGGAC 
CCCACCAATG GCTACTACAA CGTGCGTGCC CATGAAGACC GCCCGTCTTC CAGGGCAGTG 
CTCTATGCTG ACTACCGTGC CCCTGGCCCT GCCCGCTTCG ACGGCCGCCC CTCATCCCGT 
CTCTCCCACT CCAGCGGCTA TGCCCAGCTC AACACCTATA GCCGGGGCCC TGCCTCTGAC 
TATGGCCCTG AGCCCACACC CCCTGGCCCT GCTGCCCCAG CTGGCACTGA CACAACCAGC 
CAGCTGTCCT ACGAGAACTA TGAGAAGTTC AACTCCCATC CCTTCCCTGG GGCAGCTGGG 
TACCCCACCT ACCGACTGGG CTACCCCCAG GCCCCACCCT CTGGCCTGGA GCGGACCCCA 
TATGAGGCGT ATGACCCCAT TGGCAAGTAC GCCACAGCCA CTCGATTCTC CTACACCTCC 
CAGCACTCGG ACTACGGCCA GCGATTCCAG CAGCGCATGC AGACTCACGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273278.1
CDS	405..2378
Translation	MLSLLVWILTLSDTFSQVPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV

Target ORF information:

RefSeq Version	NM_001286349.1
Organism	Homo sapiens(human)
Definition	Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286349.1

ORF Insert Sequence:

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGTCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGTCCTG AGTAACAGCA ACCAGCTGCT GCTGAAGTCG 
GTGACTCAGG CAGACGCTGG CACCTACACC TGCCGGGCCA TCGTGCCTCG AATCGGAGTG 
GCTGAGCGGG AGGTGCCGCT CTATGTGAAC GGGCCCCCCA TCATCTCCAG TGAGGCAGTG 
CAGTATGCTG TGAGGGGTGA CGGTGGCAAG GTGGAGTGTT TCATTGGGAG CACACCACCC 
CCAGACCGCA TAGCATGGGC CTGGAAGGAG AACTTCTTGG AGGTGGGGAC CCTGGAACGC 
TATACAGTGG AGAGGACCAA CTCAGGCAGT GGGGTGCTAT CCACGCTCAC CATCAACAAT 
GTCATGGAGG CCGACTTTCA GACTCACTAC AACTGCACCG CCTGGAACAG CTTCGGGCCA 
GGCACAGCCA TCATCCAGCT GGAAGAGCGA GAGGTGTTAC CTGTGGGCAT CATAGCTGGG 
GCCACCATCG GCGCGAGCAT CCTGCTCATC TTCTTCTTCA TCGCCTTGGT ATTCTTCCTC 
TACCGGCGCC GCAAAGGCAG TCGCAAAGAC GTGACCCTGA GGAAGCTGGA TATCAAGGTG 
GAGACAGTGA ACCGAGAGCC ACTTACGATG CATTCTGACC GGGAGGATGA CACCGCCAGC 
GTCTCCACAG CAACCCGGGT CATGAAGGCC ATCTACTCGT CGTTTAAGGA TGATGTGGAT 
CTGAAGCAGG ACCTGCGCTG CGACACCATC GACACCCGGG AGGAGTATGA GATGAAGGAC 
CCCACCAATG GCTACTACAA CGTGCGTGCC CATGAAGACC GCCCGTCTTC CAGGGCAGTG 
CTCTATGCTG ACTACCGTGC CCCTGGCCCT GCCCGCTTCG ACGGCCGCCC CTCATCCCGT 
CTCTCCCACT CCAGCGGCTA TGCCCAGCTC AACACCTATA GCCGGGGCCC TGCCTCTGAC 
TATGGCCCTG AGCCCACACC CCCTGGCCCT GCTGCCCCAG CTGGCACTGA CACAACCAGC 
CAGCTGTCCT ACGAGAACTA TGAGAAGTTC AACTCCCATC CCTTCCCTGG GGCAGCTGGG 
TACCCCACCT ACCGACTGGG CTACCCCCAG GCCCCACCCT CTGGCCTGGA GCGGACCCCA 
TATGAGGCGT ATGACCCCAT TGGCAAGTAC GCCACAGCCA CTCGATTCTC CTACACCTCC 
CAGCACTCGG ACTACGGCCA GCGATTCCAG CAGCGCATGC AGACTCACGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIRREL1 ( XM_005245305.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245362.1 Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant X1, mRNA.

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CloneID	OHu34525
Clone ID Related Accession (Same CDS sequence)	XM_005245305.5 , XM_005245305.4
Accession Version	XM_005245305.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	kin of IRRE-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245305.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGGGACCCAG 
ACCCGCTTCA GCCAGGAGCC AGCTGACCAG ACGGTGGTGG CTGGACAGCG GGCCGTGCTC 
CCCTGTGTGC TGCTCAACTA CTCTGGAATT GTGCAATGGA CCAAGGACGG GCTGGCCCTG 
GGCATGGGCC AGGGCCTCAA AGCCTGGCCA CGGTACCGGG TTGTGGGCTC CGCAGACGCT 
GGGCAGTACA ACCTGGAGAT CACAGATGCT GAGCTCTCTG ACGACGCCTC TTACGAGTGC 
CAGGCCACGG AGGCCGCCCT GCGCTCTCGG CGGGCCAAAC TCACCGTGCT CATCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGGGCCC AGGCCTCCTG GCTCCCCACC CGAGGCTGCT 
CTCTCTGCCC AGGTCCTGAG TAACAGCAAC CAGCTGCTGC TGAAGTCGGT GACTCAGGCA 
GACGCTGGCA CCTACACCTG CCGGGCCATC GTGCCTCGAA TCGGAGTGGC TGAGCGGGAG 
GTGCCGCTCT ATGTGAACGG GCCCCCCATC ATCTCCAGTG AGGCAGTGCA GTATGCTGTG 
AGGGGTGACG GTGGCAAGGT GGAGTGTTTC ATTGGGAGCA CACCACCCCC AGACCGCATA 
GCATGGGCCT GGAAGGAGAA CTTCTTGGAG GTGGGGACCC TGGAACGCTA TACAGTGGAG 
AGGACCAACT CAGGCAGTGG GGTGCTATCC ACGCTCACCA TCAACAATGT CATGGAGGCC 
GACTTTCAGA CTCACTACAA CTGCACCGCC TGGAACAGCT TCGGGCCAGG CACAGCCATC 
ATCCAGCTGG AAGAGCGAGA GGTGTTACCT GTGGGCATCA TAGCTGGGGC CACCATCGGC 
GCGAGCATCC TGCTCATCTT CTTCTTCATC GCCTTGGTAT TCTTCCTCTA CCGGCGCCGC 
AAAGGCAGTC GCAAAGACGT GACCCTGAGG AAGCTGGATA TCAAGGTGGA GACAGTGAAC 
CGAGAGCCAC TTACGATGCA TTCTGACCGG GAGGATGACA CCGCCAGCGT CTCCACAGCA 
ACCCGGGTCA TGAAGGCCAT CTACTCGTCG TTTAAGGATG ATGTGGATCT GAAGCAGGAC 
CTGCGCTGCG ACACCATCGA CACCCGGGAG GAGTATGAGA TGAAGGACCC CACCAATGGC 
TACTACAACG TGCGTGCCCA TGAAGACCGC CCGTCTTCCA GGGCAGTGCT CTATGCTGAC 
TACCGTGCCC CTGGCCCTGC CCGCTTCGAC GGCCGCCCCT CATCCCGTCT CTCCCACTCC 
AGCGGCTATG CCCAGCTCAA CACCTATAGC CGGGGCCCTG CCTCTGACTA TGGCCCTGAG 
CCCACACCCC CTGGCCCTGC TGCCCCAGCT GGCACTGACA CAACCAGCCA GCTGTCCTAC 
GAGAACTATG AGAAGTTCAA CTCCCATCCC TTCCCTGGGG CAGCTGGGTA CCCCACCTAC 
CGACTGGGCT ACCCCCAGGC CCCACCCTCT GGCCTGGAGC GGACCCCATA TGAGGCGTAT 
GACCCCATTG GCAAGTACGC CACAGCCACT CGATTCTCCT ACACCTCCCA GCACTCGGAC 
TACGGCCAGC GATTCCAGCA GCGCATGCAG ACTCACGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245362.1
CDS	65..2386
Translation	MLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMGPRPPGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV

Target ORF information:

RefSeq Version	XM_005245305.5
Organism	Homo sapiens(human)
Definition	Homo sapiens kirre like nephrin family adhesion molecule 1 (KIRREL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245305.5

ORF Insert Sequence:

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGGGACCCAG 
ACCCGCTTCA GCCAGGAGCC AGCTGACCAG ACGGTGGTGG CTGGACAGCG GGCCGTGCTC 
CCCTGTGTGC TGCTCAACTA CTCTGGAATT GTGCAATGGA CCAAGGACGG GCTGGCCCTG 
GGCATGGGCC AGGGCCTCAA AGCCTGGCCA CGGTACCGGG TTGTGGGCTC CGCAGACGCT 
GGGCAGTACA ACCTGGAGAT CACAGATGCT GAGCTCTCTG ACGACGCCTC TTACGAGTGC 
CAGGCCACGG AGGCCGCCCT GCGCTCTCGG CGGGCCAAAC TCACCGTGCT CATCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGGGCCC AGGCCTCCTG GCTCCCCACC CGAGGCTGCT 
CTCTCTGCCC AGGTCCTGAG TAACAGCAAC CAGCTGCTGC TGAAGTCGGT GACTCAGGCA 
GACGCTGGCA CCTACACCTG CCGGGCCATC GTGCCTCGAA TCGGAGTGGC TGAGCGGGAG 
GTGCCGCTCT ATGTGAACGG GCCCCCCATC ATCTCCAGTG AGGCAGTGCA GTATGCTGTG 
AGGGGTGACG GTGGCAAGGT GGAGTGTTTC ATTGGGAGCA CACCACCCCC AGACCGCATA 
GCATGGGCCT GGAAGGAGAA CTTCTTGGAG GTGGGGACCC TGGAACGCTA TACAGTGGAG 
AGGACCAACT CAGGCAGTGG GGTGCTATCC ACGCTCACCA TCAACAATGT CATGGAGGCC 
GACTTTCAGA CTCACTACAA CTGCACCGCC TGGAACAGCT TCGGGCCAGG CACAGCCATC 
ATCCAGCTGG AAGAGCGAGA GGTGTTACCT GTGGGCATCA TAGCTGGGGC CACCATCGGC 
GCGAGCATCC TGCTCATCTT CTTCTTCATC GCCTTGGTAT TCTTCCTCTA CCGGCGCCGC 
AAAGGCAGTC GCAAAGACGT GACCCTGAGG AAGCTGGATA TCAAGGTGGA GACAGTGAAC 
CGAGAGCCAC TTACGATGCA TTCTGACCGG GAGGATGACA CCGCCAGCGT CTCCACAGCA 
ACCCGGGTCA TGAAGGCCAT CTACTCGTCG TTTAAGGATG ATGTGGATCT GAAGCAGGAC 
CTGCGCTGCG ACACCATCGA CACCCGGGAG GAGTATGAGA TGAAGGACCC CACCAATGGC 
TACTACAACG TGCGTGCCCA TGAAGACCGC CCGTCTTCCA GGGCAGTGCT CTATGCTGAC 
TACCGTGCCC CTGGCCCTGC CCGCTTCGAC GGCCGCCCCT CATCCCGTCT CTCCCACTCC 
AGCGGCTATG CCCAGCTCAA CACCTATAGC CGGGGCCCTG CCTCTGACTA TGGCCCTGAG 
CCCACACCCC CTGGCCCTGC TGCCCCAGCT GGCACTGACA CAACCAGCCA GCTGTCCTAC 
GAGAACTATG AGAAGTTCAA CTCCCATCCC TTCCCTGGGG CAGCTGGGTA CCCCACCTAC 
CGACTGGGCT ACCCCCAGGC CCCACCCTCT GGCCTGGAGC GGACCCCATA TGAGGCGTAT 
GACCCCATTG GCAAGTACGC CACAGCCACT CGATTCTCCT ACACCTCCCA GCACTCGGAC 
TACGGCCAGC GATTCCAGCA GCGCATGCAG ACTCACGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIRREL1 ( XM_005245305.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245362.1 Homo sapiens kin of IRRE like (Drosophila) (KIRREL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu34525
Clone ID Related Accession (Same CDS sequence)	XM_005245305.5 , XM_005245305.4
Accession Version	XM_005245305.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	kin of IRRE-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245305.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGGGACCCAG 
ACCCGCTTCA GCCAGGAGCC AGCTGACCAG ACGGTGGTGG CTGGACAGCG GGCCGTGCTC 
CCCTGTGTGC TGCTCAACTA CTCTGGAATT GTGCAATGGA CCAAGGACGG GCTGGCCCTG 
GGCATGGGCC AGGGCCTCAA AGCCTGGCCA CGGTACCGGG TTGTGGGCTC CGCAGACGCT 
GGGCAGTACA ACCTGGAGAT CACAGATGCT GAGCTCTCTG ACGACGCCTC TTACGAGTGC 
CAGGCCACGG AGGCCGCCCT GCGCTCTCGG CGGGCCAAAC TCACCGTGCT CATCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGGGCCC AGGCCTCCTG GCTCCCCACC CGAGGCTGCT 
CTCTCTGCCC AGGTCCTGAG TAACAGCAAC CAGCTGCTGC TGAAGTCGGT GACTCAGGCA 
GACGCTGGCA CCTACACCTG CCGGGCCATC GTGCCTCGAA TCGGAGTGGC TGAGCGGGAG 
GTGCCGCTCT ATGTGAACGG GCCCCCCATC ATCTCCAGTG AGGCAGTGCA GTATGCTGTG 
AGGGGTGACG GTGGCAAGGT GGAGTGTTTC ATTGGGAGCA CACCACCCCC AGACCGCATA 
GCATGGGCCT GGAAGGAGAA CTTCTTGGAG GTGGGGACCC TGGAACGCTA TACAGTGGAG 
AGGACCAACT CAGGCAGTGG GGTGCTATCC ACGCTCACCA TCAACAATGT CATGGAGGCC 
GACTTTCAGA CTCACTACAA CTGCACCGCC TGGAACAGCT TCGGGCCAGG CACAGCCATC 
ATCCAGCTGG AAGAGCGAGA GGTGTTACCT GTGGGCATCA TAGCTGGGGC CACCATCGGC 
GCGAGCATCC TGCTCATCTT CTTCTTCATC GCCTTGGTAT TCTTCCTCTA CCGGCGCCGC 
AAAGGCAGTC GCAAAGACGT GACCCTGAGG AAGCTGGATA TCAAGGTGGA GACAGTGAAC 
CGAGAGCCAC TTACGATGCA TTCTGACCGG GAGGATGACA CCGCCAGCGT CTCCACAGCA 
ACCCGGGTCA TGAAGGCCAT CTACTCGTCG TTTAAGGATG ATGTGGATCT GAAGCAGGAC 
CTGCGCTGCG ACACCATCGA CACCCGGGAG GAGTATGAGA TGAAGGACCC CACCAATGGC 
TACTACAACG TGCGTGCCCA TGAAGACCGC CCGTCTTCCA GGGCAGTGCT CTATGCTGAC 
TACCGTGCCC CTGGCCCTGC CCGCTTCGAC GGCCGCCCCT CATCCCGTCT CTCCCACTCC 
AGCGGCTATG CCCAGCTCAA CACCTATAGC CGGGGCCCTG CCTCTGACTA TGGCCCTGAG 
CCCACACCCC CTGGCCCTGC TGCCCCAGCT GGCACTGACA CAACCAGCCA GCTGTCCTAC 
GAGAACTATG AGAAGTTCAA CTCCCATCCC TTCCCTGGGG CAGCTGGGTA CCCCACCTAC 
CGACTGGGCT ACCCCCAGGC CCCACCCTCT GGCCTGGAGC GGACCCCATA TGAGGCGTAT 
GACCCCATTG GCAAGTACGC CACAGCCACT CGATTCTCCT ACACCTCCCA GCACTCGGAC 
TACGGCCAGC GATTCCAGCA GCGCATGCAG ACTCACGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245362.1
CDS	62..2383
Translation	MLSLLVWILTLSDTFSQGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEDTRIDGGPVILLQAGTPHNLTCRAFNAKPAATIIWFRDGTQQEGAVASTELLKDGKRETTVSQLLINPTDLDIGRVFTCRSMNEAIPSGKETSIELDVHHPPTVTLSIEPQTVQEGERVVFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVHNKVGSTNVSTLVNVHFAPRIVVDPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMGPRPPGSPPEAALSAQVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQYAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVLPVGIIAGATIGASILLIFFFIALVFFLYRRRKGSRKDVTLRKLDIKVETVNREPLTMHSDREDDTASVSTATRVMKAIYSSFKDDVDLKQDLRCDTIDTREEYEMKDPTNGYYNVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV

Target ORF information:

RefSeq Version	XM_005245305.4
Organism	Homo sapiens(human)
Definition	Homo sapiens kin of IRRE like (Drosophila) (KIRREL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245305.4

ORF Insert Sequence:

ATGCTGAGCC TCCTCGTCTG GATCCTCACT CTCTCCGATA CTTTCTCCCA AGGGACCCAG 
ACCCGCTTCA GCCAGGAGCC AGCTGACCAG ACGGTGGTGG CTGGACAGCG GGCCGTGCTC 
CCCTGTGTGC TGCTCAACTA CTCTGGAATT GTGCAATGGA CCAAGGACGG GCTGGCCCTG 
GGCATGGGCC AGGGCCTCAA AGCCTGGCCA CGGTACCGGG TTGTGGGCTC CGCAGACGCT 
GGGCAGTACA ACCTGGAGAT CACAGATGCT GAGCTCTCTG ACGACGCCTC TTACGAGTGC 
CAGGCCACGG AGGCCGCCCT GCGCTCTCGG CGGGCCAAAC TCACCGTGCT CATCCCCCCA 
GAGGACACCA GGATTGACGG AGGCCCTGTG ATTCTACTGC AGGCAGGCAC CCCCCACAAC 
CTCACATGCC GGGCCTTCAA TGCGAAGCCT GCTGCCACCA TCATCTGGTT CCGGGACGGG 
ACGCAGCAGG AGGGCGCTGT GGCCAGCACG GAATTGCTGA AGGATGGGAA GAGGGAGACC 
ACCGTGAGCC AACTGCTTAT TAACCCCACG GACCTGGACA TAGGGCGTGT CTTCACTTGC 
CGAAGCATGA ACGAAGCCAT CCCTAGTGGC AAGGAGACTT CCATCGAGCT GGATGTGCAC 
CACCCTCCTA CAGTGACCCT GTCCATTGAG CCACAGACGG TGCAGGAGGG TGAGCGTGTT 
GTCTTTACCT GCCAGGCCAC AGCCAACCCC GAGATCTTGG GCTACAGGTG GGCCAAAGGG 
GGTTTCTTGA TTGAAGACGC CCACGAGAGT CGCTATGAGA CAAATGTGGA TTATTCCTTT 
TTCACGGAGC CTGTGTCTTG TGAGGTTCAC AACAAAGTGG GAAGCACCAA TGTCAGCACT 
TTAGTAAATG TCCACTTTGC TCCCCGGATT GTAGTTGACC CCAAACCCAC AACCACAGAC 
ATTGGCTCTG ATGTGACCCT TACCTGTGTC TGGGTTGGGA ATCCCCCCCT CACTCTCACC 
TGGACCAAAA AGGACTCAAA TATGGGGCCC AGGCCTCCTG GCTCCCCACC CGAGGCTGCT 
CTCTCTGCCC AGGTCCTGAG TAACAGCAAC CAGCTGCTGC TGAAGTCGGT GACTCAGGCA 
GACGCTGGCA CCTACACCTG CCGGGCCATC GTGCCTCGAA TCGGAGTGGC TGAGCGGGAG 
GTGCCGCTCT ATGTGAACGG GCCCCCCATC ATCTCCAGTG AGGCAGTGCA GTATGCTGTG 
AGGGGTGACG GTGGCAAGGT GGAGTGTTTC ATTGGGAGCA CACCACCCCC AGACCGCATA 
GCATGGGCCT GGAAGGAGAA CTTCTTGGAG GTGGGGACCC TGGAACGCTA TACAGTGGAG 
AGGACCAACT CAGGCAGTGG GGTGCTATCC ACGCTCACCA TCAACAATGT CATGGAGGCC 
GACTTTCAGA CTCACTACAA CTGCACCGCC TGGAACAGCT TCGGGCCAGG CACAGCCATC 
ATCCAGCTGG AAGAGCGAGA GGTGTTACCT GTGGGCATCA TAGCTGGGGC CACCATCGGC 
GCGAGCATCC TGCTCATCTT CTTCTTCATC GCCTTGGTAT TCTTCCTCTA CCGGCGCCGC 
AAAGGCAGTC GCAAAGACGT GACCCTGAGG AAGCTGGATA TCAAGGTGGA GACAGTGAAC 
CGAGAGCCAC TTACGATGCA TTCTGACCGG GAGGATGACA CCGCCAGCGT CTCCACAGCA 
ACCCGGGTCA TGAAGGCCAT CTACTCGTCG TTTAAGGATG ATGTGGATCT GAAGCAGGAC 
CTGCGCTGCG ACACCATCGA CACCCGGGAG GAGTATGAGA TGAAGGACCC CACCAATGGC 
TACTACAACG TGCGTGCCCA TGAAGACCGC CCGTCTTCCA GGGCAGTGCT CTATGCTGAC 
TACCGTGCCC CTGGCCCTGC CCGCTTCGAC GGCCGCCCCT CATCCCGTCT CTCCCACTCC 
AGCGGCTATG CCCAGCTCAA CACCTATAGC CGGGGCCCTG CCTCTGACTA TGGCCCTGAG 
CCCACACCCC CTGGCCCTGC TGCCCCAGCT GGCACTGACA CAACCAGCCA GCTGTCCTAC 
GAGAACTATG AGAAGTTCAA CTCCCATCCC TTCCCTGGGG CAGCTGGGTA CCCCACCTAC 
CGACTGGGCT ACCCCCAGGC CCCACCCTCT GGCCTGGAGC GGACCCCATA TGAGGCGTAT 
GACCCCATTG GCAAGTACGC CACAGCCACT CGATTCTCCT ACACCTCCCA GCACTCGGAC 
TACGGCCAGC GATTCCAGCA GCGCATGCAG ACTCACGTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural Analysis of the Myo1c and Neph1 Complex Provides Insight into the Intracellular Movement of Neph1.
Molecular and cellular biology36(11)1639-54(2016 06)
Arif E,Sharma P,Solanki A,Mallik L,Rathore YS,Twal WO,Nath SK,Gandhi D,Holzman LB,Ostap EM,Ashish,Nihalani D

Slit diaphragm protein Neph1 and its signaling: a novel therapeutic target for protection of podocytes against glomerular injury.
The Journal of biological chemistry289(14)9502-18(2014 Apr)
Arif E,Rathore YS,Kumari B,Ashish F,Wong HN,Holzman LB,Nihalani D

Solution structure analysis of cytoplasmic domain of podocyte protein Neph1 using small/wide angle x-ray scattering (SWAXS).
The Journal of biological chemistry287(12)9441-53(2012 Mar)
Mallik L,Arif E,Sharma P,Rathore YS,Wong HN,Holzman LB,Ashish,Nihalani D

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

Motor protein Myo1c is a podocyte protein that facilitates the transport of slit diaphragm protein Neph1 to the podocyte membrane.
Molecular and cellular biology31(10)2134-50(2011 May)
Arif E,Wagner MC,Johnstone DB,Wong HN,George B,Pruthi PA,Lazzara MJ,Nihalani D

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KIRREL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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