rst cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	rst
Entrez Gene ID	31290
Full Name	roughest
Synonyms	CG4125,CT13684,Dmel\CG4125,IREE-C,IRREC/RST,Irre-C,IrreC,IrreC-Rst,IrreC-rst,IrreC/Rst,IrrecC,Rst,Rst/IrreC,UB883,irreC,irreC-rst,irrecC-rst,rst-irreC,rst-irrecC,rst/irrecC
General protein information	Preferred Names roughest Names CG4125 gene product from transcript CG4125-RB C-Roughest CG4125-PA CG4125-PB ChiasmC-roughest Irregular-chiasm-C irreC-roughest irregular chiasm C-roughest irregular chiasm C-roughest protein irregular chiasm-C-roughest irregular chiasmC-roughest irregular optic chiasma C irregular optic chiasma C/Roughest rst-PA rst-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 3C3-3C4\|1-2.2 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001297906.1	NP_001284835.1	roughest, isoform B
NM_080319.5	NP_525058.2	roughest, isoform A

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KIRREL	XP_005640973.1
	KIRREL	XP_001116954.1
Danio rerio (zebrafish)	kirrelb	NP_001124123.1
Pan troglodytes (chimpanzee)	KIRREL	XP_003308572.1
Bos taurus (cattle)	KIRREL	XP_003585870.2
Rattus norvegicus (Norway rat)	Kirrel	NP_997489.1
Danio rerio (zebrafish)	kirrela	NP_998033.1
Xenopus tropicalis (tropical clawed frog)	kirrel	XP_002938994.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000218	XP_310916.5
Homo sapiens (human)	KIRREL	NP_060710.3
Mus musculus (house mouse)	Kirrel	NP_570937.2
Gallus gallus (chicken)	KIRREL	XP_004948232.1
Drosophila melanogaster (fruit fly)	rst	NP_525058.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the roles of Rst, Hbs, Kirre and SNS in the spacing of bristles of the anterior wing margin, are explored.
Title: The Cell Adhesion Molecules Roughest, Hibris, Kin of Irre and Sticks and Stones Are Required for Long Range Spacing of the Drosophila Wing Disc Sensory Sensilla.

Cindr recapitulates both proposed functions of its mammalian orthologs Cd2ap and Cin85: targeting the IgCAMs Rst and Hbs for endocytosis and stabilizing these heterophilic IgCAM complexes.
Title: Interactions between Drosophila IgCAM adhesion receptors and cindr, the Cd2ap/Cin85 ortholog.

Analysis of a cytoplasmic motif present in Kirre and IrreC/Rst revealed a functionally required, conserved 12 AA motif (KIN1 motif).
Title: Functional study of mammalian Neph proteins in Drosophila melanogaster.

Findings suggest a general mechanism by which rst and kirre expression could be fine tuned to optimize their redundant roles during development.
Title: rst transcriptional activity influences kirre mRNA concentration in the Drosophila pupal retina during the final steps of ommatidial patterning.

identified two cell-adhesion molecules: Hibris, which is expressed in post-mitotic lamina precursor cells; and Roughest, which is expressed on retinal axons.
Title: Recognition of pre- and postsynaptic neurons via nephrin/NEPH1 homologs is a basis for the formation of the Drosophila retinotopic map.

The following rst gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rst cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa00674	NM_080319.5 Latest version!	Drosophila melanogaster roughest (rst), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa00674	NM_001297906.1 Latest version!	Drosophila melanogaster roughest (rst), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rst ( NM_001297906.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284835.1 Drosophila melanogaster roughest (rst), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa00674
Clone ID Related Accession (Same CDS sequence)	NM_001297906.1 , NM_080319.5
Accession Version	NM_001297906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roughest, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCACA CGATGCAGCT GCTCCTGCTG GCCACTATTG TGGGCATGGT CAGGAGCTCG 
CCGTACACGA GCTATCAGAA CCAACGGTTC GCCATGGAGC CGCAGGATCA GACGGCAGTG 
GTTGGGGCCA GGGTAACGCT ACCCTGCCGA GTGATCAACA AACAGGGCAC GCTCCAATGG 
ACCAAGGATG ACTTTGGACT TGGCACGTCC CGGGATCTGA GTGGATTCGA ACGCTACGCG 
ATGGTGGGCA GTGACGAGGA GGGTGACTAC TCCCTGGACA TTTATCCAGT GATGCTGGAC 
GACGATGCTC GTTACCAGTG CCAAGTGAGC CCAGGTCCCG AGGGCCAACC AGCCATTAGG 
TCCACATTCG CCGGATTGAC GGTGCTCGTT CCGCCCGAGG CGCCCAAAAT CACACAGGGC 
GACGTCATCT ATGCCACCGA GGATCGCAAA GTGGAGATCG AGTGCGTTTC GGTTGGCGGA 
AAGCCGGCTG CTGAGATTAC CTGGATTGAT GGCCTGGGCA ATGTCCTTAC GGATAACATT 
GAGTACACGG TGATACCGCT GCCCGATCAG CGACGCTTTA CGGCCAAGTC CGTCCTGCGA 
TTGACCCCCA AAAAGGAACA CCACAACACG AACTTCAGTT GCCAGGCGCA GAACACGGCG 
GACCGCACCT ATCGCTCGGC GAAAATACGT GTCGAGGTGA AATATGCCCC CAAGGTGAAG 
GTGAATGTGA TGGGCTCGCT TCCCGGCGGT GCAGGTGGTT CGGTTGGTGG TGCAGGCGGT 
GGCAGTGTCC ACATGAGCAC CGGTTCCCGT ATTGTGGAGC ATTCGCAGGT GCGCTTGGAG 
TGCCGGGCAG ATGCGAATCC CAGCGATGTC CGGTACCGCT GGTTCATAAA CGACGAACCG 
ATCATCGGCG GCCAGAAGAC AGAGATGGTG ATACGCAATG TGACTCGCAA GTTCCACGAT 
GCGATTGTCA AGTGCGAGGT GCAGAATTCC GTGGGCAAGA GTGAGGACAG CGAAACCCTT 
GACATAAGCT ATGCTCCCAG TTTCCGGCAG CGACCACAGT CAATGGAGGC GGACGTTGGC 
AGCGTGGTGT CCCTAACCTG CGAGGTGGAC AGCAATCCGC AGCCGGAGAT CGTCTGGATA 
CAGCATCCCA GTGACCGGGT GGTCGGCACC AGCACAAATC TCACATTCAG TGTGAGCAAT 
GAGACGGCTG GAAGGTACTA CTGCAAGGCC AATGTACCCG GATACGCTGA GATTTCGGCA 
GATGCCTATG TCTATCTGAA GGGCTCGCCG GCGATTGGAT CGCAGAGGAC ACAGTACGGA 
CTGGTGGGAG ATACGGCTCG GATCGAATGC TTTGCCAGCA GTGTTCCTCG AGCCCGTCAC 
GTCTCGTGGA CGTTCAACGG CCAGGAGATC AGCTCGGAAT CGGGACACGA CTATTCGATT 
CTGGTTGATG CCGTGCCGGG GGGCGTGAAG AGCACGCTCA TCATTAGGGA CAGCCAGGCC 
TACCACTACG GAAAGTACAA CTGCACGGTG GTCAACGATT ACGGCAACGA CGTGGCCGAG 
ATTCAGCTGC AAGCCAAGAA GAGCGTTTCC CTGCTGATGA CAATTGTGGG CGGCATTTCG 
GTGGTGGCCT TCCTGCTGGT GCTGACCATT TTGGTTGTGG TCTACATCAA GTGTAAGAAG 
CGCACCAAGC TGCCGCCAGC GGATGTGATA AGCGAGCATC AGATCACGAA AAATGGCGGC 
GTTAGCTGCA AACTGGAACC AGGCGACCGG ACCTCGAACT ACAGCGATCT AAAGGTGGAC 
ATTTCGGGCG GCTATGTGCC CTACGGCGAC TACAGTACGC ACTACAGTCC GCCTCCGCAA 
TACCTGACCA CCTGTTCGAC GAAATCCAAT GGCAGCTCGA CCATTATGCA GAACAACCAT 
CAGAACCAAT TGCAACTACA GCAGCAGCAG CAACAGAGCC ACCACCAGCA CCACACACAG 
ACGACGACCC TGCCGATGAC CTTCCTGACC AACAGCAGCG GTGGCAGCTT GACTGGCAGT 
ATTATTGGAT CCCGTGAAAT TCGCCAGGAC AACGGGCTGC CCAGTCTGCA GTCGACCACC 
GCCTCGGTGG TTAGCTCATC GCCGAATGGC AGCTGCAGCA ATCAGAGCAC CACTGCCGCC 
ACCACCACCA CCACCCACGT GGTGGTGCCC AGCTCGATGG CCCTGAGTGT GGATCCCCGC 
TATAGCGCCA TTTACGGCAA TCCCTATCTT AGGTCCTCCA ACTCGTCGCT GCTGCCGCCA 
CCCACTGCCG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284835.1
CDS	541..2835
Translation	MLHTMQLLLLATIVGMVRSSPYTSYQNQRFAMEPQDQTAVVGARVTLPCRVINKQGTLQWTKDDFGLGTSRDLSGFERYAMVGSDEEGDYSLDIYPVMLDDDARYQCQVSPGPEGQPAIRSTFAGLTVLVPPEAPKITQGDVIYATEDRKVEIECVSVGGKPAAEITWIDGLGNVLTDNIEYTVIPLPDQRRFTAKSVLRLTPKKEHHNTNFSCQAQNTADRTYRSAKIRVEVKYAPKVKVNVMGSLPGGAGGSVGGAGGGSVHMSTGSRIVEHSQVRLECRADANPSDVRYRWFINDEPIIGGQKTEMVIRNVTRKFHDAIVKCEVQNSVGKSEDSETLDISYAPSFRQRPQSMEADVGSVVSLTCEVDSNPQPEIVWIQHPSDRVVGTSTNLTFSVSNETAGRYYCKANVPGYAEISADAYVYLKGSPAIGSQRTQYGLVGDTARIECFASSVPRARHVSWTFNGQEISSESGHDYSILVDAVPGGVKSTLIIRDSQAYHYGKYNCTVVNDYGNDVAEIQLQAKKSVSLLMTIVGGISVVAFLLVLTILVVVYIKCKKRTKLPPADVISEHQITKNGGVSCKLEPGDRTSNYSDLKVDISGGYVPYGDYSTHYSPPPQYLTTCSTKSNGSSTIMQNNHQNQLQLQQQQQQSHHQHHTQTTTLPMTFLTNSSGGSLTGSIIGSREIRQDNGLPSLQSTTASVVSSSPNGSCSNQSTTAATTTTTHVVVPSSMALSVDPRYSAIYGNPYLRSSNSSLLPPPTAV

Target ORF information:

RefSeq Version	NM_001297906.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roughest (rst), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297906.1

ORF Insert Sequence:

ATGTTGCACA CGATGCAGCT GCTCCTGCTG GCCACTATTG TGGGCATGGT CAGGAGCTCG 
CCGTACACGA GCTATCAGAA CCAACGGTTC GCCATGGAGC CGCAGGATCA GACGGCAGTG 
GTTGGGGCCA GGGTAACGCT ACCCTGCCGA GTGATCAACA AACAGGGCAC GCTCCAATGG 
ACCAAGGATG ACTTTGGACT TGGCACGTCC CGGGATCTGA GTGGATTCGA ACGCTACGCG 
ATGGTGGGCA GTGACGAGGA GGGTGACTAC TCCCTGGACA TTTATCCAGT GATGCTGGAC 
GACGATGCTC GTTACCAGTG CCAAGTGAGC CCAGGTCCCG AGGGCCAACC AGCCATTAGG 
TCCACATTCG CCGGATTGAC GGTGCTCGTT CCGCCCGAGG CGCCCAAAAT CACACAGGGC 
GACGTCATCT ATGCCACCGA GGATCGCAAA GTGGAGATCG AGTGCGTTTC GGTTGGCGGA 
AAGCCGGCTG CTGAGATTAC CTGGATTGAT GGCCTGGGCA ATGTCCTTAC GGATAACATT 
GAGTACACGG TGATACCGCT GCCCGATCAG CGACGCTTTA CGGCCAAGTC CGTCCTGCGA 
TTGACCCCCA AAAAGGAACA CCACAACACG AACTTCAGTT GCCAGGCGCA GAACACGGCG 
GACCGCACCT ATCGCTCGGC GAAAATACGT GTCGAGGTGA AATATGCCCC CAAGGTGAAG 
GTGAATGTGA TGGGCTCGCT TCCCGGCGGT GCAGGTGGTT CGGTTGGTGG TGCAGGCGGT 
GGCAGTGTCC ACATGAGCAC CGGTTCCCGT ATTGTGGAGC ATTCGCAGGT GCGCTTGGAG 
TGCCGGGCAG ATGCGAATCC CAGCGATGTC CGGTACCGCT GGTTCATAAA CGACGAACCG 
ATCATCGGCG GCCAGAAGAC AGAGATGGTG ATACGCAATG TGACTCGCAA GTTCCACGAT 
GCGATTGTCA AGTGCGAGGT GCAGAATTCC GTGGGCAAGA GTGAGGACAG CGAAACCCTT 
GACATAAGCT ATGCTCCCAG TTTCCGGCAG CGACCACAGT CAATGGAGGC GGACGTTGGC 
AGCGTGGTGT CCCTAACCTG CGAGGTGGAC AGCAATCCGC AGCCGGAGAT CGTCTGGATA 
CAGCATCCCA GTGACCGGGT GGTCGGCACC AGCACAAATC TCACATTCAG TGTGAGCAAT 
GAGACGGCTG GAAGGTACTA CTGCAAGGCC AATGTACCCG GATACGCTGA GATTTCGGCA 
GATGCCTATG TCTATCTGAA GGGCTCGCCG GCGATTGGAT CGCAGAGGAC ACAGTACGGA 
CTGGTGGGAG ATACGGCTCG GATCGAATGC TTTGCCAGCA GTGTTCCTCG AGCCCGTCAC 
GTCTCGTGGA CGTTCAACGG CCAGGAGATC AGCTCGGAAT CGGGACACGA CTATTCGATT 
CTGGTTGATG CCGTGCCGGG GGGCGTGAAG AGCACGCTCA TCATTAGGGA CAGCCAGGCC 
TACCACTACG GAAAGTACAA CTGCACGGTG GTCAACGATT ACGGCAACGA CGTGGCCGAG 
ATTCAGCTGC AAGCCAAGAA GAGCGTTTCC CTGCTGATGA CAATTGTGGG CGGCATTTCG 
GTGGTGGCCT TCCTGCTGGT GCTGACCATT TTGGTTGTGG TCTACATCAA GTGTAAGAAG 
CGCACCAAGC TGCCGCCAGC GGATGTGATA AGCGAGCATC AGATCACGAA AAATGGCGGC 
GTTAGCTGCA AACTGGAACC AGGCGACCGG ACCTCGAACT ACAGCGATCT AAAGGTGGAC 
ATTTCGGGCG GCTATGTGCC CTACGGCGAC TACAGTACGC ACTACAGTCC GCCTCCGCAA 
TACCTGACCA CCTGTTCGAC GAAATCCAAT GGCAGCTCGA CCATTATGCA GAACAACCAT 
CAGAACCAAT TGCAACTACA GCAGCAGCAG CAACAGAGCC ACCACCAGCA CCACACACAG 
ACGACGACCC TGCCGATGAC CTTCCTGACC AACAGCAGCG GTGGCAGCTT GACTGGCAGT 
ATTATTGGAT CCCGTGAAAT TCGCCAGGAC AACGGGCTGC CCAGTCTGCA GTCGACCACC 
GCCTCGGTGG TTAGCTCATC GCCGAATGGC AGCTGCAGCA ATCAGAGCAC CACTGCCGCC 
ACCACCACCA CCACCCACGT GGTGGTGCCC AGCTCGATGG CCCTGAGTGT GGATCCCCGC 
TATAGCGCCA TTTACGGCAA TCCCTATCTT AGGTCCTCCA ACTCGTCGCT GCTGCCGCCA 
CCCACTGCCG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rst ( NM_080319.5 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525058.2 Drosophila melanogaster roughest (rst), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa00674
Clone ID Related Accession (Same CDS sequence)	NM_001297906.1 , NM_080319.5
Accession Version	NM_080319.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roughest, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080319.4. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTGCACA CGATGCAGCT GCTCCTGCTG GCCACTATTG TGGGCATGGT CAGGAGCTCG 
CCGTACACGA GCTATCAGAA CCAACGGTTC GCCATGGAGC CGCAGGATCA GACGGCAGTG 
GTTGGGGCCA GGGTAACGCT ACCCTGCCGA GTGATCAACA AACAGGGCAC GCTCCAATGG 
ACCAAGGATG ACTTTGGACT TGGCACGTCC CGGGATCTGA GTGGATTCGA ACGCTACGCG 
ATGGTGGGCA GTGACGAGGA GGGTGACTAC TCCCTGGACA TTTATCCAGT GATGCTGGAC 
GACGATGCTC GTTACCAGTG CCAAGTGAGC CCAGGTCCCG AGGGCCAACC AGCCATTAGG 
TCCACATTCG CCGGATTGAC GGTGCTCGTT CCGCCCGAGG CGCCCAAAAT CACACAGGGC 
GACGTCATCT ATGCCACCGA GGATCGCAAA GTGGAGATCG AGTGCGTTTC GGTTGGCGGA 
AAGCCGGCTG CTGAGATTAC CTGGATTGAT GGCCTGGGCA ATGTCCTTAC GGATAACATT 
GAGTACACGG TGATACCGCT GCCCGATCAG CGACGCTTTA CGGCCAAGTC CGTCCTGCGA 
TTGACCCCCA AAAAGGAACA CCACAACACG AACTTCAGTT GCCAGGCGCA GAACACGGCG 
GACCGCACCT ATCGCTCGGC GAAAATACGT GTCGAGGTGA AATATGCCCC CAAGGTGAAG 
GTGAATGTGA TGGGCTCGCT TCCCGGCGGT GCAGGTGGTT CGGTTGGTGG TGCAGGCGGT 
GGCAGTGTCC ACATGAGCAC CGGTTCCCGT ATTGTGGAGC ATTCGCAGGT GCGCTTGGAG 
TGCCGGGCAG ATGCGAATCC CAGCGATGTC CGGTACCGCT GGTTCATAAA CGACGAACCG 
ATCATCGGCG GCCAGAAGAC AGAGATGGTG ATACGCAATG TGACTCGCAA GTTCCACGAT 
GCGATTGTCA AGTGCGAGGT GCAGAATTCC GTGGGCAAGA GTGAGGACAG CGAAACCCTT 
GACATAAGCT ATGCTCCCAG TTTCCGGCAG CGACCACAGT CAATGGAGGC GGACGTTGGC 
AGCGTGGTGT CCCTAACCTG CGAGGTGGAC AGCAATCCGC AGCCGGAGAT CGTCTGGATA 
CAGCATCCCA GTGACCGGGT GGTCGGCACC AGCACAAATC TCACATTCAG TGTGAGCAAT 
GAGACGGCTG GAAGGTACTA CTGCAAGGCC AATGTACCCG GATACGCTGA GATTTCGGCA 
GATGCCTATG TCTATCTGAA GGGCTCGCCG GCGATTGGAT CGCAGAGGAC ACAGTACGGA 
CTGGTGGGAG ATACGGCTCG GATCGAATGC TTTGCCAGCA GTGTTCCTCG AGCCCGTCAC 
GTCTCGTGGA CGTTCAACGG CCAGGAGATC AGCTCGGAAT CGGGACACGA CTATTCGATT 
CTGGTTGATG CCGTGCCGGG GGGCGTGAAG AGCACGCTCA TCATTAGGGA CAGCCAGGCC 
TACCACTACG GAAAGTACAA CTGCACGGTG GTCAACGATT ACGGCAACGA CGTGGCCGAG 
ATTCAGCTGC AAGCCAAGAA GAGCGTTTCC CTGCTGATGA CAATTGTGGG CGGCATTTCG 
GTGGTGGCCT TCCTGCTGGT GCTGACCATT TTGGTTGTGG TCTACATCAA GTGTAAGAAG 
CGCACCAAGC TGCCGCCAGC GGATGTGATA AGCGAGCATC AGATCACGAA AAATGGCGGC 
GTTAGCTGCA AACTGGAACC AGGCGACCGG ACCTCGAACT ACAGCGATCT AAAGGTGGAC 
ATTTCGGGCG GCTATGTGCC CTACGGCGAC TACAGTACGC ACTACAGTCC GCCTCCGCAA 
TACCTGACCA CCTGTTCGAC GAAATCCAAT GGCAGCTCGA CCATTATGCA GAACAACCAT 
CAGAACCAAT TGCAACTACA GCAGCAGCAG CAACAGAGCC ACCACCAGCA CCACACACAG 
ACGACGACCC TGCCGATGAC CTTCCTGACC AACAGCAGCG GTGGCAGCTT GACTGGCAGT 
ATTATTGGAT CCCGTGAAAT TCGCCAGGAC AACGGGCTGC CCAGTCTGCA GTCGACCACC 
GCCTCGGTGG TTAGCTCATC GCCGAATGGC AGCTGCAGCA ATCAGAGCAC CACTGCCGCC 
ACCACCACCA CCACCCACGT GGTGGTGCCC AGCTCGATGG CCCTGAGTGT GGATCCCCGC 
TATAGCGCCA TTTACGGCAA TCCCTATCTT AGGTCCTCCA ACTCGTCGCT GCTGCCGCCA 
CCCACTGCCG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525058.2
CDS	541..2835
Translation	MLHTMQLLLLATIVGMVRSSPYTSYQNQRFAMEPQDQTAVVGARVTLPCRVINKQGTLQWTKDDFGLGTSRDLSGFERYAMVGSDEEGDYSLDIYPVMLDDDARYQCQVSPGPEGQPAIRSTFAGLTVLVPPEAPKITQGDVIYATEDRKVEIECVSVGGKPAAEITWIDGLGNVLTDNIEYTVIPLPDQRRFTAKSVLRLTPKKEHHNTNFSCQAQNTADRTYRSAKIRVEVKYAPKVKVNVMGSLPGGAGGSVGGAGGGSVHMSTGSRIVEHSQVRLECRADANPSDVRYRWFINDEPIIGGQKTEMVIRNVTRKFHDAIVKCEVQNSVGKSEDSETLDISYAPSFRQRPQSMEADVGSVVSLTCEVDSNPQPEIVWIQHPSDRVVGTSTNLTFSVSNETAGRYYCKANVPGYAEISADAYVYLKGSPAIGSQRTQYGLVGDTARIECFASSVPRARHVSWTFNGQEISSESGHDYSILVDAVPGGVKSTLIIRDSQAYHYGKYNCTVVNDYGNDVAEIQLQAKKSVSLLMTIVGGISVVAFLLVLTILVVVYIKCKKRTKLPPADVISEHQITKNGGVSCKLEPGDRTSNYSDLKVDISGGYVPYGDYSTHYSPPPQYLTTCSTKSNGSSTIMQNNHQNQLQLQQQQQQSHHQHHTQTTTLPMTFLTNSSGGSLTGSIIGSREIRQDNGLPSLQSTTASVVSSSPNGSCSNQSTTAATTTTTHVVVPSSMALSVDPRYSAIYGNPYLRSSNSSLLPPPTAV

Target ORF information:

RefSeq Version	NM_080319.5
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roughest (rst), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080319.5

ORF Insert Sequence:

ATGTTGCACA CGATGCAGCT GCTCCTGCTG GCCACTATTG TGGGCATGGT CAGGAGCTCG 
CCGTACACGA GCTATCAGAA CCAACGGTTC GCCATGGAGC CGCAGGATCA GACGGCAGTG 
GTTGGGGCCA GGGTAACGCT ACCCTGCCGA GTGATCAACA AACAGGGCAC GCTCCAATGG 
ACCAAGGATG ACTTTGGACT TGGCACGTCC CGGGATCTGA GTGGATTCGA ACGCTACGCG 
ATGGTGGGCA GTGACGAGGA GGGTGACTAC TCCCTGGACA TTTATCCAGT GATGCTGGAC 
GACGATGCTC GTTACCAGTG CCAAGTGAGC CCAGGTCCCG AGGGCCAACC AGCCATTAGG 
TCCACATTCG CCGGATTGAC GGTGCTCGTT CCGCCCGAGG CGCCCAAAAT CACACAGGGC 
GACGTCATCT ATGCCACCGA GGATCGCAAA GTGGAGATCG AGTGCGTTTC GGTTGGCGGA 
AAGCCGGCTG CTGAGATTAC CTGGATTGAT GGCCTGGGCA ATGTCCTTAC GGATAACATT 
GAGTACACGG TGATACCGCT GCCCGATCAG CGACGCTTTA CGGCCAAGTC CGTCCTGCGA 
TTGACCCCCA AAAAGGAACA CCACAACACG AACTTCAGTT GCCAGGCGCA GAACACGGCG 
GACCGCACCT ATCGCTCGGC GAAAATACGT GTCGAGGTGA AATATGCCCC CAAGGTGAAG 
GTGAATGTGA TGGGCTCGCT TCCCGGCGGT GCAGGTGGTT CGGTTGGTGG TGCAGGCGGT 
GGCAGTGTCC ACATGAGCAC CGGTTCCCGT ATTGTGGAGC ATTCGCAGGT GCGCTTGGAG 
TGCCGGGCAG ATGCGAATCC CAGCGATGTC CGGTACCGCT GGTTCATAAA CGACGAACCG 
ATCATCGGCG GCCAGAAGAC AGAGATGGTG ATACGCAATG TGACTCGCAA GTTCCACGAT 
GCGATTGTCA AGTGCGAGGT GCAGAATTCC GTGGGCAAGA GTGAGGACAG CGAAACCCTT 
GACATAAGCT ATGCTCCCAG TTTCCGGCAG CGACCACAGT CAATGGAGGC GGACGTTGGC 
AGCGTGGTGT CCCTAACCTG CGAGGTGGAC AGCAATCCGC AGCCGGAGAT CGTCTGGATA 
CAGCATCCCA GTGACCGGGT GGTCGGCACC AGCACAAATC TCACATTCAG TGTGAGCAAT 
GAGACGGCTG GAAGGTACTA CTGCAAGGCC AATGTACCCG GATACGCTGA GATTTCGGCA 
GATGCCTATG TCTATCTGAA GGGCTCGCCG GCGATTGGAT CGCAGAGGAC ACAGTACGGA 
CTGGTGGGAG ATACGGCTCG GATCGAATGC TTTGCCAGCA GTGTTCCTCG AGCCCGTCAC 
GTCTCGTGGA CGTTCAACGG CCAGGAGATC AGCTCGGAAT CGGGACACGA CTATTCGATT 
CTGGTTGATG CCGTGCCGGG GGGCGTGAAG AGCACGCTCA TCATTAGGGA CAGCCAGGCC 
TACCACTACG GAAAGTACAA CTGCACGGTG GTCAACGATT ACGGCAACGA CGTGGCCGAG 
ATTCAGCTGC AAGCCAAGAA GAGCGTTTCC CTGCTGATGA CAATTGTGGG CGGCATTTCG 
GTGGTGGCCT TCCTGCTGGT GCTGACCATT TTGGTTGTGG TCTACATCAA GTGTAAGAAG 
CGCACCAAGC TGCCGCCAGC GGATGTGATA AGCGAGCATC AGATCACGAA AAATGGCGGC 
GTTAGCTGCA AACTGGAACC AGGCGACCGG ACCTCGAACT ACAGCGATCT AAAGGTGGAC 
ATTTCGGGCG GCTATGTGCC CTACGGCGAC TACAGTACGC ACTACAGTCC GCCTCCGCAA 
TACCTGACCA CCTGTTCGAC GAAATCCAAT GGCAGCTCGA CCATTATGCA GAACAACCAT 
CAGAACCAAT TGCAACTACA GCAGCAGCAG CAACAGAGCC ACCACCAGCA CCACACACAG 
ACGACGACCC TGCCGATGAC CTTCCTGACC AACAGCAGCG GTGGCAGCTT GACTGGCAGT 
ATTATTGGAT CCCGTGAAAT TCGCCAGGAC AACGGGCTGC CCAGTCTGCA GTCGACCACC 
GCCTCGGTGG TTAGCTCATC GCCGAATGGC AGCTGCAGCA ATCAGAGCAC CACTGCCGCC 
ACCACCACCA CCACCCACGT GGTGGTGCCC AGCTCGATGG CCCTGAGTGT GGATCCCCGC 
TATAGCGCCA TTTACGGCAA TCCCTATCTT AGGTCCTCCA ACTCGTCGCT GCTGCCGCCA 
CCCACTGCCG TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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rst cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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