CDCA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDCA4
Entrez Gene ID	55038
Full Name	cell division cycle associated 4
Synonyms	HEPP,SEI-3/HEPP
General protein information	Preferred Names cell division cycle associated 4 Names cell division cycle-associated protein 4 hematopoietic progenitor protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.33
Summary	This gene encodes a protein that belongs to the E2F family of transcription factors. This protein regulates E2F-dependent transcriptional activation and cell proliferation, mainly through the E2F/retinoblastoma protein pathway. It also functions in the regulation of JUN oncogene expression. This protein shows distinctive nuclear-mitotic apparatus distribution, it is involved in spindle organization from prometaphase, and may also play a role as a midzone factor involved in chromosome segregation or cytokinesis. Two alternatively spliced transcript variants encoding the same protein have been noted for this gene. Two pseudogenes have also been identified on chromosome 1. [provided by RefSeq, May 2014].
Disorder MIM:	612270

mRNA and Protein(s)

mRNA	Protein	Name
NM_145701.3	NP_663747.1	cell division cycle-associated protein 4
NM_145701.2	NP_663747.1	cell division cycle-associated protein 4
NM_017955.3	NP_060425.2	cell division cycle-associated protein 4
NM_017955.4	NP_060425.2	cell division cycle-associated protein 4

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	cdca4	NP_001016649.1
	LOC708068	XP_001096509.1
Bos taurus (cattle)	CDCA4	XP_005222391.1
Mus musculus (house mouse)	Cdca4	NP_082299.1
Pan troglodytes (chimpanzee)	CDCA4	XP_001141461.2
Rattus norvegicus (Norway rat)	Cdca4	NP_001032291.1
Canis lupus familiaris (dog)	CDCA4	XP_005623939.1
Gallus gallus (chicken)	CDCA4	NP_001026383.2
Homo sapiens (human)	CDCA4	NP_663747.1
Danio rerio (zebrafish)	si:dkey-177p2.7	NP_001008580.1

Gene Ontology
Bibliography

Related articles in PubMed

CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF?7/ADM human breast cancer cell line.
Xu Y, Wu X, Li F, Huang D, Zhu W
Molecular medicine reports17(1)1507-1512(2018 Jan)

MicroRNA-15a inhibits the growth and invasiveness of malignant melanoma and directly targets on CDCA4 gene.
Alderman C, Sehlaoui A, Xiao Z, Yang Y
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13941-13950(2016 Oct)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

A Y2H-seq approach defines the human protein methyltransferase interactome.
Weimann M, Grossmann A, Woodsmith J, ?zkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U
Nature methods10(4)339-42(2013 Apr)

Transcriptional co-factor CDCA4 participates in the regulation of JUN oncogene expression.
Tategu M, Nakagawa H, Hayashi R, Yoshida K
Biochimie90(10)1515-22(2008 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High CDCA4 expression is associated with malignant melanoma.
Title: MicroRNA-15a inhibits the growth and invasiveness of malignant melanoma and directly targets on CDCA4 gene.

an important role of CDCA4 in the context of transcriptional regulation and cell fate determination through the JUN oncogene
Title: Transcriptional co-factor CDCA4 participates in the regulation of JUN oncogene expression.

Data suggest that CDCA4 is involved in spindle organization from prometaphase, and when anaphase begins, it may play a different role as a midzone factor involved in chromosome segregation or cytokinesis.
Title: The spindle function of CDCA4.

CDCA4 participates in the regulation of cell proliferation, mainly through the E2F/retinoblastoma protein pathway
Title: CDCA4 is an E2F transcription factor family-induced nuclear factor that regulates E2F-dependent transcriptional activation and cell proliferation.

Isolation of the mouse ortholog and comparison to the human protein.
Title: Cloning and characterization of Hepp, a novel gene expressed preferentially in hematopoietic progenitors and mature blood cells.

The following CDCA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDCA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25707	NM_145701.3 Latest version!	Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu25707	NM_017955.4 Latest version!	Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu25707	NM_145701.2	Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu25707	NM_017955.3	Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDCA4 ( NM_145701.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_663747.1 Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25707
Clone ID Related Accession (Same CDS sequence)	NM_145701.3 , NM_145701.2 , NM_017955.3 , NM_017955.4
Accession Version	NM_145701.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	cell division cycle-associated protein 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN427719.1, BC011736.2, AL512802.2 and BG354577.1. On May 31, 2019 this sequence version replaced NM_145701.2. Transcript Variant: This variant (2) lacks a segment in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC011736.2, SRR1163657.267970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGCAC GAGGACTGAA GAGGAAATGT GTTGGCCACG AGGAAGACGT GGAGGGAGCC 
CTGGCCGGCT TGAAGACAGT GTCCTCATAC AGCCTGCAGC GGCAGTCGCT CCTGGACATG 
TCTCTGGTGA AGTTGCAGCT TTGCCACATG CTTGTGGAGC CCAACCTGTG CCGCTCAGTC 
CTCATTGCCA ACACGGTCCG GCAGATCCAA GAGGAGATGA CGCAGGATGG GACGTGGCGC 
ACAGTGGCAC CCCAGGCTGC AGAGCGGGCG CCGCTCGACC GCTTGGTCTC CACGGAGATC 
CTGTGCCGTG CAGCGTGGGG GCAAGAGGGG GCACATCCTG CTCCTGGCTT GGGGGACGGC 
CACACACAGG GTCCAGTTTC TGACCTTTGC CCAGTCACCT CAGCACAGGC ACCAAGGCAC 
CTGCAGAGCA GCGCCTGGGA GATGGATGGC CCTCGAGAAA ACAGAGGAAG CTTTCACAAG 
TCACTTGATC AGATATTTGA AACGCTGGAG ACTAAAAACC CCAGCTGCAT GGAAGAGCTG 
TTCTCAGACG TGGACAGCCC CTACTACGAC CTGGACACAG TACTGACAGG CATGATGGGG 
GGTGCCAGGC CGGGCCCCTG CGAAGGGCTC GAGGGCTTGG CTCCGGCCAC CCCAGGCCCT 
AGCTCCAGCT GCAAGTCCGA CCTGGGCGAG CTGGACCACG TGGTGGAGAT CCTGGTGGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663747.1
CDS	92..817
Translation	MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGTWRTVAPQAAERAPLDRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRHLQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGLEGLAPATPGPSSSCKSDLGELDHVVEILVET

Target ORF information:

RefSeq Version	NM_145701.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145701.3

ORF Insert Sequence:

ATGTTTGCAC GAGGACTGAA GAGGAAATGT GTTGGCCACG AGGAAGACGT GGAGGGAGCC 
CTGGCCGGCT TGAAGACAGT GTCCTCATAC AGCCTGCAGC GGCAGTCGCT CCTGGACATG 
TCTCTGGTGA AGTTGCAGCT TTGCCACATG CTTGTGGAGC CCAACCTGTG CCGCTCAGTC 
CTCATTGCCA ACACGGTCCG GCAGATCCAA GAGGAGATGA CGCAGGATGG GACGTGGCGC 
ACAGTGGCAC CCCAGGCTGC AGAGCGGGCG CCGCTCGACC GCTTGGTCTC CACGGAGATC 
CTGTGCCGTG CAGCGTGGGG GCAAGAGGGG GCACATCCTG CTCCTGGCTT GGGGGACGGC 
CACACACAGG GTCCAGTTTC TGACCTTTGC CCAGTCACCT CAGCACAGGC ACCAAGGCAC 
CTGCAGAGCA GCGCCTGGGA GATGGATGGC CCTCGAGAAA ACAGAGGAAG CTTTCACAAG 
TCACTTGATC AGATATTTGA AACGCTGGAG ACTAAAAACC CCAGCTGCAT GGAAGAGCTG 
TTCTCAGACG TGGACAGCCC CTACTACGAC CTGGACACAG TACTGACAGG CATGATGGGG 
GGTGCCAGGC CGGGCCCCTG CGAAGGGCTC GAGGGCTTGG CTCCGGCCAC CCCAGGCCCT 
AGCTCCAGCT GCAAGTCCGA CCTGGGCGAG CTGGACCACG TGGTGGAGAT CCTGGTGGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDCA4 ( NM_145701.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_663747.1 Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25707
Clone ID Related Accession (Same CDS sequence)	NM_145701.3 , NM_145701.2 , NM_017955.3 , NM_017955.4
Accession Version	NM_145701.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	cell division cycle-associated protein 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN427719.1, BC011736.2, AL512802.2 and BG354577.1. On Sep 6, 2008 this sequence version replaced NM_145701.1. Transcript Variant: This variant (2) lacks a segment in the 3' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC011736.2, SRR1163657.267970.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGCAC GAGGACTGAA GAGGAAATGT GTTGGCCACG AGGAAGACGT GGAGGGAGCC 
CTGGCCGGCT TGAAGACAGT GTCCTCATAC AGCCTGCAGC GGCAGTCGCT CCTGGACATG 
TCTCTGGTGA AGTTGCAGCT TTGCCACATG CTTGTGGAGC CCAACCTGTG CCGCTCAGTC 
CTCATTGCCA ACACGGTCCG GCAGATCCAA GAGGAGATGA CGCAGGATGG GACGTGGCGC 
ACAGTGGCAC CCCAGGCTGC AGAGCGGGCG CCGCTCGACC GCTTGGTCTC CACGGAGATC 
CTGTGCCGTG CAGCGTGGGG GCAAGAGGGG GCACATCCTG CTCCTGGCTT GGGGGACGGC 
CACACACAGG GTCCAGTTTC TGACCTTTGC CCAGTCACCT CAGCACAGGC ACCAAGGCAC 
CTGCAGAGCA GCGCCTGGGA GATGGATGGC CCTCGAGAAA ACAGAGGAAG CTTTCACAAG 
TCACTTGATC AGATATTTGA AACGCTGGAG ACTAAAAACC CCAGCTGCAT GGAAGAGCTG 
TTCTCAGACG TGGACAGCCC CTACTACGAC CTGGACACAG TACTGACAGG CATGATGGGG 
GGTGCCAGGC CGGGCCCCTG CGAAGGGCTC GAGGGCTTGG CTCCGGCCAC CCCAGGCCCT 
AGCTCCAGCT GCAAGTCCGA CCTGGGCGAG CTGGACCACG TGGTGGAGAT CCTGGTGGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_663747.1
CDS	97..822
Translation	MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGTWRTVAPQAAERAPLDRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRHLQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGLEGLAPATPGPSSSCKSDLGELDHVVEILVET

Target ORF information:

RefSeq Version	NM_145701.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145701.2

ORF Insert Sequence:

ATGTTTGCAC GAGGACTGAA GAGGAAATGT GTTGGCCACG AGGAAGACGT GGAGGGAGCC 
CTGGCCGGCT TGAAGACAGT GTCCTCATAC AGCCTGCAGC GGCAGTCGCT CCTGGACATG 
TCTCTGGTGA AGTTGCAGCT TTGCCACATG CTTGTGGAGC CCAACCTGTG CCGCTCAGTC 
CTCATTGCCA ACACGGTCCG GCAGATCCAA GAGGAGATGA CGCAGGATGG GACGTGGCGC 
ACAGTGGCAC CCCAGGCTGC AGAGCGGGCG CCGCTCGACC GCTTGGTCTC CACGGAGATC 
CTGTGCCGTG CAGCGTGGGG GCAAGAGGGG GCACATCCTG CTCCTGGCTT GGGGGACGGC 
CACACACAGG GTCCAGTTTC TGACCTTTGC CCAGTCACCT CAGCACAGGC ACCAAGGCAC 
CTGCAGAGCA GCGCCTGGGA GATGGATGGC CCTCGAGAAA ACAGAGGAAG CTTTCACAAG 
TCACTTGATC AGATATTTGA AACGCTGGAG ACTAAAAACC CCAGCTGCAT GGAAGAGCTG 
TTCTCAGACG TGGACAGCCC CTACTACGAC CTGGACACAG TACTGACAGG CATGATGGGG 
GGTGCCAGGC CGGGCCCCTG CGAAGGGCTC GAGGGCTTGG CTCCGGCCAC CCCAGGCCCT 
AGCTCCAGCT GCAAGTCCGA CCTGGGCGAG CTGGACCACG TGGTGGAGAT CCTGGTGGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDCA4 ( NM_017955.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060425.2 Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25707
Clone ID Related Accession (Same CDS sequence)	NM_145701.3 , NM_145701.2 , NM_017955.3 , NM_017955.4
Accession Version	NM_017955.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-20
Organism	Homo sapiens(human)
Product	cell division cycle-associated protein 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN427719.1, AF322239.1, AL512802.2 and BG354577.1. On Sep 6, 2008 this sequence version replaced NM_017955.2. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BG354577.1, SRR1660809.107568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGCAC GAGGACTGAA GAGGAAATGT GTTGGCCACG AGGAAGACGT GGAGGGAGCC 
CTGGCCGGCT TGAAGACAGT GTCCTCATAC AGCCTGCAGC GGCAGTCGCT CCTGGACATG 
TCTCTGGTGA AGTTGCAGCT TTGCCACATG CTTGTGGAGC CCAACCTGTG CCGCTCAGTC 
CTCATTGCCA ACACGGTCCG GCAGATCCAA GAGGAGATGA CGCAGGATGG GACGTGGCGC 
ACAGTGGCAC CCCAGGCTGC AGAGCGGGCG CCGCTCGACC GCTTGGTCTC CACGGAGATC 
CTGTGCCGTG CAGCGTGGGG GCAAGAGGGG GCACATCCTG CTCCTGGCTT GGGGGACGGC 
CACACACAGG GTCCAGTTTC TGACCTTTGC CCAGTCACCT CAGCACAGGC ACCAAGGCAC 
CTGCAGAGCA GCGCCTGGGA GATGGATGGC CCTCGAGAAA ACAGAGGAAG CTTTCACAAG 
TCACTTGATC AGATATTTGA AACGCTGGAG ACTAAAAACC CCAGCTGCAT GGAAGAGCTG 
TTCTCAGACG TGGACAGCCC CTACTACGAC CTGGACACAG TACTGACAGG CATGATGGGG 
GGTGCCAGGC CGGGCCCCTG CGAAGGGCTC GAGGGCTTGG CTCCGGCCAC CCCAGGCCCT 
AGCTCCAGCT GCAAGTCCGA CCTGGGCGAG CTGGACCACG TGGTGGAGAT CCTGGTGGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060425.2
CDS	97..822
Translation	MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGTWRTVAPQAAERAPLDRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRHLQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGLEGLAPATPGPSSSCKSDLGELDHVVEILVET

Target ORF information:

RefSeq Version	NM_017955.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017955.3

ORF Insert Sequence:

ATGTTTGCAC GAGGACTGAA GAGGAAATGT GTTGGCCACG AGGAAGACGT GGAGGGAGCC 
CTGGCCGGCT TGAAGACAGT GTCCTCATAC AGCCTGCAGC GGCAGTCGCT CCTGGACATG 
TCTCTGGTGA AGTTGCAGCT TTGCCACATG CTTGTGGAGC CCAACCTGTG CCGCTCAGTC 
CTCATTGCCA ACACGGTCCG GCAGATCCAA GAGGAGATGA CGCAGGATGG GACGTGGCGC 
ACAGTGGCAC CCCAGGCTGC AGAGCGGGCG CCGCTCGACC GCTTGGTCTC CACGGAGATC 
CTGTGCCGTG CAGCGTGGGG GCAAGAGGGG GCACATCCTG CTCCTGGCTT GGGGGACGGC 
CACACACAGG GTCCAGTTTC TGACCTTTGC CCAGTCACCT CAGCACAGGC ACCAAGGCAC 
CTGCAGAGCA GCGCCTGGGA GATGGATGGC CCTCGAGAAA ACAGAGGAAG CTTTCACAAG 
TCACTTGATC AGATATTTGA AACGCTGGAG ACTAAAAACC CCAGCTGCAT GGAAGAGCTG 
TTCTCAGACG TGGACAGCCC CTACTACGAC CTGGACACAG TACTGACAGG CATGATGGGG 
GGTGCCAGGC CGGGCCCCTG CGAAGGGCTC GAGGGCTTGG CTCCGGCCAC CCCAGGCCCT 
AGCTCCAGCT GCAAGTCCGA CCTGGGCGAG CTGGACCACG TGGTGGAGAT CCTGGTGGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDCA4 ( NM_017955.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060425.2 Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25707
Clone ID Related Accession (Same CDS sequence)	NM_145701.3 , NM_145701.2 , NM_017955.3 , NM_017955.4
Accession Version	NM_017955.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	cell division cycle-associated protein 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN427719.1, AF322239.1, AL512802.2 and BG354577.1. On May 17, 2019 this sequence version replaced NM_017955.3. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BG354577.1, SRR1660809.107568.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000336219.4/ ENSP00000337226.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTTGCAC GAGGACTGAA GAGGAAATGT GTTGGCCACG AGGAAGACGT GGAGGGAGCC 
CTGGCCGGCT TGAAGACAGT GTCCTCATAC AGCCTGCAGC GGCAGTCGCT CCTGGACATG 
TCTCTGGTGA AGTTGCAGCT TTGCCACATG CTTGTGGAGC CCAACCTGTG CCGCTCAGTC 
CTCATTGCCA ACACGGTCCG GCAGATCCAA GAGGAGATGA CGCAGGATGG GACGTGGCGC 
ACAGTGGCAC CCCAGGCTGC AGAGCGGGCG CCGCTCGACC GCTTGGTCTC CACGGAGATC 
CTGTGCCGTG CAGCGTGGGG GCAAGAGGGG GCACATCCTG CTCCTGGCTT GGGGGACGGC 
CACACACAGG GTCCAGTTTC TGACCTTTGC CCAGTCACCT CAGCACAGGC ACCAAGGCAC 
CTGCAGAGCA GCGCCTGGGA GATGGATGGC CCTCGAGAAA ACAGAGGAAG CTTTCACAAG 
TCACTTGATC AGATATTTGA AACGCTGGAG ACTAAAAACC CCAGCTGCAT GGAAGAGCTG 
TTCTCAGACG TGGACAGCCC CTACTACGAC CTGGACACAG TACTGACAGG CATGATGGGG 
GGTGCCAGGC CGGGCCCCTG CGAAGGGCTC GAGGGCTTGG CTCCGGCCAC CCCAGGCCCT 
AGCTCCAGCT GCAAGTCCGA CCTGGGCGAG CTGGACCACG TGGTGGAGAT CCTGGTGGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060425.2
CDS	92..817
Translation	MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGTWRTVAPQAAERAPLDRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRHLQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGLEGLAPATPGPSSSCKSDLGELDHVVEILVET

Target ORF information:

RefSeq Version	NM_017955.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle associated 4 (CDCA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017955.4

ORF Insert Sequence:

ATGTTTGCAC GAGGACTGAA GAGGAAATGT GTTGGCCACG AGGAAGACGT GGAGGGAGCC 
CTGGCCGGCT TGAAGACAGT GTCCTCATAC AGCCTGCAGC GGCAGTCGCT CCTGGACATG 
TCTCTGGTGA AGTTGCAGCT TTGCCACATG CTTGTGGAGC CCAACCTGTG CCGCTCAGTC 
CTCATTGCCA ACACGGTCCG GCAGATCCAA GAGGAGATGA CGCAGGATGG GACGTGGCGC 
ACAGTGGCAC CCCAGGCTGC AGAGCGGGCG CCGCTCGACC GCTTGGTCTC CACGGAGATC 
CTGTGCCGTG CAGCGTGGGG GCAAGAGGGG GCACATCCTG CTCCTGGCTT GGGGGACGGC 
CACACACAGG GTCCAGTTTC TGACCTTTGC CCAGTCACCT CAGCACAGGC ACCAAGGCAC 
CTGCAGAGCA GCGCCTGGGA GATGGATGGC CCTCGAGAAA ACAGAGGAAG CTTTCACAAG 
TCACTTGATC AGATATTTGA AACGCTGGAG ACTAAAAACC CCAGCTGCAT GGAAGAGCTG 
TTCTCAGACG TGGACAGCCC CTACTACGAC CTGGACACAG TACTGACAGG CATGATGGGG 
GGTGCCAGGC CGGGCCCCTG CGAAGGGCTC GAGGGCTTGG CTCCGGCCAC CCCAGGCCCT 
AGCTCCAGCT GCAAGTCCGA CCTGGGCGAG CTGGACCACG TGGTGGAGAT CCTGGTGGAG 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CDCA4, a downstream gene of the Nrf2 signaling pathway, regulates cell proliferation and apoptosis in the MCF?7/ADM human breast cancer cell line.
Molecular medicine reports17(1)1507-1512(2018 Jan)
Xu Y,Wu X,Li F,Huang D,Zhu W

MicroRNA-15a inhibits the growth and invasiveness of malignant melanoma and directly targets on CDCA4 gene.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(10)13941-13950(2016 Oct)
Alderman C,Sehlaoui A,Xiao Z,Yang Y

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

A Y2H-seq approach defines the human protein methyltransferase interactome.
Nature methods10(4)339-42(2013 Apr)
Weimann M,Grossmann A,Woodsmith J,?zkan Z,Birth P,Meierhofer D,Benlasfer N,Valovka T,Timmermann B,Wanker EE,Sauer S,Stelzl U

Transcriptional co-factor CDCA4 participates in the regulation of JUN oncogene expression.
Biochimie90(10)1515-22(2008 Oct)
Tategu M,Nakagawa H,Hayashi R,Yoshida K

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CDCA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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