NECAB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NECAB2
Entrez Gene ID	54550
Full Name	N-terminal EF-hand calcium binding protein 2
Synonyms	EFCBP2,stip-2
General protein information	Preferred Names N-terminal EF-hand calcium binding protein 2 Names N-terminal EF-hand calcium-binding protein 2 EF-hand calcium-binding protein 2 neuronal calcium binding 2 neuronal calcium-binding protein 2 synaptotagmin-interacting protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q23.3
Summary	The protein encoded by this gene is a neuronal calcium-binding protein that binds to and modulates the function of at least two receptors, adenosine A(2A) receptor and metabotropic glutamate receptor type 5. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001329749.1	NP_001316678.1	N-terminal EF-hand calcium-binding protein 2 isoform 3
XM_017023317.1	XP_016878806.1	N-terminal EF-hand calcium-binding protein 2 isoform X1
XM_017023317.2	XP_016878806.1	N-terminal EF-hand calcium-binding protein 2 isoform X1
NM_019065.2	NP_061938.2	N-terminal EF-hand calcium-binding protein 2 isoform 1
NM_001329749.2	NP_001316678.1	N-terminal EF-hand calcium-binding protein 2 isoform 3
NM_001329748.1	NP_001316677.1	N-terminal EF-hand calcium-binding protein 2 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	NECAB2	NP_061938.2
Bos taurus (cattle)	NECAB2	NP_001092447.1
Danio rerio (zebrafish)	necab2	NP_001025366.1
Gallus gallus (chicken)	NECAB2	XP_414072.1
Xenopus tropicalis (tropical clawed frog)	LOC101731915	XP_004913648.1
Pan troglodytes (chimpanzee)	NECAB2	XP_001150555.1
	LOC718169	XP_001110489.2
Mus musculus (house mouse)	Necab2	NP_473436.1
Rattus norvegicus (Norway rat)	Necab2	NP_596906.1
Canis lupus familiaris (dog)	NECAB2	XP_005620942.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparative anatomical distribution of neuronal calcium-binding protein (NECAB) 1 and -2 in rodent and human spinal cord.
Zhang MD, Barde S, Szodorai E, Josephson A, Mitsios N, Watanabe M, Attems J, Lubec G, Kov?cs GG, Uhl?n M, Mulder J, Harkany T, H?kfelt T
Brain structure & function221(7)3803-23(2016 09)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

The association of metabotropic glutamate receptor type 5 with the neuronal Ca2+-binding protein 2 modulates receptor function.
Canela L, Fern?ndez-Due?as V, Albergaria C, Watanabe M, Llu?s C, Mallol J, Canela EI, Franco R, Luj?n R, Ciruela F
Journal of neurochemistry111(2)555-67(2009 Oct)

The neuronal Ca(2+) -binding protein 2 (NECAB2) interacts with the adenosine A(2A) receptor and modulates the cell surface expression and function of the receptor.
Canela L, Luj?n R, Llu?s C, Burgue?o J, Mallol J, Canela EI, Franco R, Ciruela F
Molecular and cellular neurosciences36(1)1-12(2007 Sep)

NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern.
Sugita S, Ho A, S?dhof TC
Neuroscience112(1)51-63(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study suggests that the phenotypic segregation of Neuronal calcium-binding protein 1 and -2 (NECAB1 and -2) to respective excitatory and inhibitory spinal systems can underpin functional modalities in determining the fidelity of synaptic neurotransmission and neuronal responsiveness, and might bear translational relevance to humans.
Title: Comparative anatomical distribution of neuronal calcium-binding protein (NECAB) 1 and -2 in rodent and human spinal cord.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

NECAB2 by its physical interaction with mGlu(5b) receptor modulates receptor function
Title: The association of metabotropic glutamate receptor type 5 with the neuronal Ca2+-binding protein 2 modulates receptor function.

Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Molecular genetics of successful smoking cessation: convergent genome-wide association study results.

The following NECAB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECAB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02428	NM_019065.2 Latest version!	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu68453	NM_001329749.2 Latest version!	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu86085	XM_017023317.2 Latest version!	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu101805	NM_001329748.1 Latest version!	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu86085	XM_017023317.1	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu68453	NM_001329749.1	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NECAB2 ( NM_019065.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_061938.2 Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu02428
Clone ID Related Accession (Same CDS sequence)	NM_019065.2
Accession Version	NM_019065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	N-terminal EF-hand calcium-binding protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY299331.1. On Jun 20, 2003 this sequence version replaced NM_019065.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AY299331.1, AK127376.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGCGAGC GGGCGGCGCG CCTGTGCAGG GCCGGCGCGC ACAGGCTGCT CCGGGAGCCG 
CCGCAGCAGG GCCGGGCGCT GGGCGGGCTG CTGCGCTGGG TGGGCGCCAG GATGGGCGAG 
CCCCGGGAGT CGCTGGCCCC CGCCGCCCCC GCGGACCCCG GCCCAGCCTC GCCGCGCGGG 
GGCACCGCCG TCATCCTGGA CATTTTCCGC CGTGCGGACA AAAATGATGA TGGGAAGCTG 
TCCTTGGAGG AATTCCAGCT CTTCTTTGCA GATGGCGTCC TTAATGAGAA AGAACTGGAG 
GATCTCTTTC ACACGATTGA CTCTGACAAC ACCAACCATG TGGACACCAA GGAGCTGTGT 
GATTACTTTG TGGACCACAT GGGTGACTAT GAGGATGTCC TGGCCTCCCT GGAGACCTTG 
AATCACTCTG TCCTGAAGGC CATGGGTTAT ACCAAGAAGG TATATGAGGG TGGGAGCAAC 
GTGGACCAGT TTGTGACCCG CTTCCTCCTG AAGGAGACGG CCAATCAGAT CCAGTCGCTG 
CTGAGCTCAG TGGAGAGTGC GGTGGAGGCC ATCGAGGAAC AGACCAGCCA GCTCCGACAG 
AACCACATCA AACCCAGCCA CAGCGCGGCA CAGACCTGGT GTGGAAGCCC CACTCCCGCC 
TCTGCCCCCA ACCACAAGCT CATGGCTATG GAACAAGGCA AGACCCTTCC ATCTGCCACG 
GAGGATGCAA AGGAAGAGGG TCTGGAAGCC CAGATCAGCC GCTTGGCAGA GCTGATTGGG 
AGGCTGGAGA GCAAAGCACT GTGGTTCGAC CTGCAGCAGC GCCTGTCAGA TGAAGATGGC 
ACCAACATGC ACCTGCAGCT GGTCCGGCAG GAGATGGCCG TGTGCCCCGA GCAACTGAGC 
GAGTTTCTGG ACTCTCTGCG CCAGTATCTG CGGGGGACCA CTGGCGTGAG GAACTGCTTC 
CACATCACTG CCGTGAGGCT CTCAGATGGC TTCACCTTTG TCATCTATGA GTTCTGGGAG 
ACAGAGGAGG CGTGGAAGAG GCACCTGCAG AGCCCCCTGT GTAAGGCGTT CCGGCACGTC 
AAGGTGGACA CACTGAGCCA GCCTGAGGCC CTCTCCAGGA TCTTGGTGCC AGCTGCTTGG 
TGCACGGTGG GACGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_061938.2
CDS	18..1178
Translation	MCERAARLCRAGAHRLLREPPQQGRALGGLLRWVGARMGEPRESLAPAAPADPGPASPRGGTAVILDIFRRADKNDDGKLSLEEFQLFFADGVLNEKELEDLFHTIDSDNTNHVDTKELCDYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD

Target ORF information:

RefSeq Version	NM_019065.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019065.2

ORF Insert Sequence:

ATGTGCGAGC GGGCGGCGCG CCTGTGCAGG GCCGGCGCGC ACAGGCTGCT CCGGGAGCCG 
CCGCAGCAGG GCCGGGCGCT GGGCGGGCTG CTGCGCTGGG TGGGCGCCAG GATGGGCGAG 
CCCCGGGAGT CGCTGGCCCC CGCCGCCCCC GCGGACCCCG GCCCAGCCTC GCCGCGCGGG 
GGCACCGCCG TCATCCTGGA CATTTTCCGC CGTGCGGACA AAAATGATGA TGGGAAGCTG 
TCCTTGGAGG AATTCCAGCT CTTCTTTGCA GATGGCGTCC TTAATGAGAA AGAACTGGAG 
GATCTCTTTC ACACGATTGA CTCTGACAAC ACCAACCATG TGGACACCAA GGAGCTGTGT 
GATTACTTTG TGGACCACAT GGGTGACTAT GAGGATGTCC TGGCCTCCCT GGAGACCTTG 
AATCACTCTG TCCTGAAGGC CATGGGTTAT ACCAAGAAGG TATATGAGGG TGGGAGCAAC 
GTGGACCAGT TTGTGACCCG CTTCCTCCTG AAGGAGACGG CCAATCAGAT CCAGTCGCTG 
CTGAGCTCAG TGGAGAGTGC GGTGGAGGCC ATCGAGGAAC AGACCAGCCA GCTCCGACAG 
AACCACATCA AACCCAGCCA CAGCGCGGCA CAGACCTGGT GTGGAAGCCC CACTCCCGCC 
TCTGCCCCCA ACCACAAGCT CATGGCTATG GAACAAGGCA AGACCCTTCC ATCTGCCACG 
GAGGATGCAA AGGAAGAGGG TCTGGAAGCC CAGATCAGCC GCTTGGCAGA GCTGATTGGG 
AGGCTGGAGA GCAAAGCACT GTGGTTCGAC CTGCAGCAGC GCCTGTCAGA TGAAGATGGC 
ACCAACATGC ACCTGCAGCT GGTCCGGCAG GAGATGGCCG TGTGCCCCGA GCAACTGAGC 
GAGTTTCTGG ACTCTCTGCG CCAGTATCTG CGGGGGACCA CTGGCGTGAG GAACTGCTTC 
CACATCACTG CCGTGAGGCT CTCAGATGGC TTCACCTTTG TCATCTATGA GTTCTGGGAG 
ACAGAGGAGG CGTGGAAGAG GCACCTGCAG AGCCCCCTGT GTAAGGCGTT CCGGCACGTC 
AAGGTGGACA CACTGAGCCA GCCTGAGGCC CTCTCCAGGA TCTTGGTGCC AGCTGCTTGG 
TGCACGGTGG GACGGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NECAB2 ( NM_001329749.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316678.1 Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu68453
Clone ID Related Accession (Same CDS sequence)	NM_001329749.1 , NM_001329749.2
Accession Version	NM_001329749.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-20
Organism	Homo sapiens(human)
Product	N-terminal EF-hand calcium-binding protein 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC040169.7. On Jul 27, 2016 this sequence version replaced XM_011523135.1. Transcript Variant: This variant (3) lacks an alternate coding exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: BC016979.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGG GAAGCTGTCC TTGGAGGAAT TCCAGCTCTT CTTTGCAGAT GGCGTCCTTA 
ATGAGAAAGA ACTGGAGGAT CTCTTTCACA CGATTGACTC TGACAACACC AAATTACTTT 
GTGGACCACA TGGGTGACTA TGAGGATGTC CTGGCCTCCC TGGAGACCTT GAATCACTCT 
GTCCTGAAGG CCATGGGTTA TACCAAGAAG GTATATGAGG GTGGGAGCAA CGTGGACCAG 
TTTGTGACCC GCTTCCTCCT GAAGGAGACG GCCAATCAGA TCCAGTCGCT GCTGAGCTCA 
GTGGAGAGTG CGGTGGAGGC CATCGAGGAA CAGACCAGCC AGCTCCGACA GAACCACATC 
AAACCCAGCC ACAGCGCGGC ACAGACCTGG TGTGGAAGCC CCACTCCCGC CTCTGCCCCC 
AACCACAAGC TCATGGCTAT GGAACAAGGC AAGACCCTTC CATCTGCCAC GGAGGATGCA 
AAGGAAGAGG GTCTGGAAGC CCAGATCAGC CGCTTGGCAG AGCTGATTGG GAGGCTGGAG 
AGCAAAGCAC TGTGGTTCGA CCTGCAGCAG CGCCTGTCAG ATGAAGATGG CACCAACATG 
CACCTGCAGC TGGTCCGGCA GGAGATGGCC GTGTGCCCCG AGCAACTGAG CGAGTTTCTG 
GACTCTCTGC GCCAGTATCT GCGGGGGACC ACTGGCGTGA GGAACTGCTT CCACATCACT 
GCCGTGAGGC TCTCAGATGG CTTCACCTTT GTCATCTATG AGTTCTGGGA GACAGAGGAG 
GCGTGGAAGA GGCACCTGCA GAGCCCCCTG TGTAAGGCGT TCCGGCACGT CAAGGTGGAC 
ACACTGAGCC AGCCTGAGGC CCTCTCCAGG ATCTTGGTGC CAGCTGCTTG GTGCACGGTG 
GGACGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316678.1
CDS	241..1152
Translation	MMMGSCPWRNSSSSLQMASLMRKNWRISFTRLTLTTPNYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD

Target ORF information:

RefSeq Version	NM_001329749.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329749.1

ORF Insert Sequence:

ATGATGATGG GAAGCTGTCC TTGGAGGAAT TCCAGCTCTT CTTTGCAGAT GGCGTCCTTA 
ATGAGAAAGA ACTGGAGGAT CTCTTTCACA CGATTGACTC TGACAACACC AAATTACTTT 
GTGGACCACA TGGGTGACTA TGAGGATGTC CTGGCCTCCC TGGAGACCTT GAATCACTCT 
GTCCTGAAGG CCATGGGTTA TACCAAGAAG GTATATGAGG GTGGGAGCAA CGTGGACCAG 
TTTGTGACCC GCTTCCTCCT GAAGGAGACG GCCAATCAGA TCCAGTCGCT GCTGAGCTCA 
GTGGAGAGTG CGGTGGAGGC CATCGAGGAA CAGACCAGCC AGCTCCGACA GAACCACATC 
AAACCCAGCC ACAGCGCGGC ACAGACCTGG TGTGGAAGCC CCACTCCCGC CTCTGCCCCC 
AACCACAAGC TCATGGCTAT GGAACAAGGC AAGACCCTTC CATCTGCCAC GGAGGATGCA 
AAGGAAGAGG GTCTGGAAGC CCAGATCAGC CGCTTGGCAG AGCTGATTGG GAGGCTGGAG 
AGCAAAGCAC TGTGGTTCGA CCTGCAGCAG CGCCTGTCAG ATGAAGATGG CACCAACATG 
CACCTGCAGC TGGTCCGGCA GGAGATGGCC GTGTGCCCCG AGCAACTGAG CGAGTTTCTG 
GACTCTCTGC GCCAGTATCT GCGGGGGACC ACTGGCGTGA GGAACTGCTT CCACATCACT 
GCCGTGAGGC TCTCAGATGG CTTCACCTTT GTCATCTATG AGTTCTGGGA GACAGAGGAG 
GCGTGGAAGA GGCACCTGCA GAGCCCCCTG TGTAAGGCGT TCCGGCACGT CAAGGTGGAC 
ACACTGAGCC AGCCTGAGGC CCTCTCCAGG ATCTTGGTGC CAGCTGCTTG GTGCACGGTG 
GGACGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NECAB2 ( NM_001329749.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316678.1 Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu68453
Clone ID Related Accession (Same CDS sequence)	NM_001329749.1 , NM_001329749.2
Accession Version	NM_001329749.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-08
Organism	Homo sapiens(human)
Product	N-terminal EF-hand calcium-binding protein 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC040169.7. On Jun 2, 2019 this sequence version replaced NM_001329749.1. Transcript Variant: This variant (3) lacks an alternate coding exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGG GAAGCTGTCC TTGGAGGAAT TCCAGCTCTT CTTTGCAGAT GGCGTCCTTA 
ATGAGAAAGA ACTGGAGGAT CTCTTTCACA CGATTGACTC TGACAACACC AAATTACTTT 
GTGGACCACA TGGGTGACTA TGAGGATGTC CTGGCCTCCC TGGAGACCTT GAATCACTCT 
GTCCTGAAGG CCATGGGTTA TACCAAGAAG GTATATGAGG GTGGGAGCAA CGTGGACCAG 
TTTGTGACCC GCTTCCTCCT GAAGGAGACG GCCAATCAGA TCCAGTCGCT GCTGAGCTCA 
GTGGAGAGTG CGGTGGAGGC CATCGAGGAA CAGACCAGCC AGCTCCGACA GAACCACATC 
AAACCCAGCC ACAGCGCGGC ACAGACCTGG TGTGGAAGCC CCACTCCCGC CTCTGCCCCC 
AACCACAAGC TCATGGCTAT GGAACAAGGC AAGACCCTTC CATCTGCCAC GGAGGATGCA 
AAGGAAGAGG GTCTGGAAGC CCAGATCAGC CGCTTGGCAG AGCTGATTGG GAGGCTGGAG 
AGCAAAGCAC TGTGGTTCGA CCTGCAGCAG CGCCTGTCAG ATGAAGATGG CACCAACATG 
CACCTGCAGC TGGTCCGGCA GGAGATGGCC GTGTGCCCCG AGCAACTGAG CGAGTTTCTG 
GACTCTCTGC GCCAGTATCT GCGGGGGACC ACTGGCGTGA GGAACTGCTT CCACATCACT 
GCCGTGAGGC TCTCAGATGG CTTCACCTTT GTCATCTATG AGTTCTGGGA GACAGAGGAG 
GCGTGGAAGA GGCACCTGCA GAGCCCCCTG TGTAAGGCGT TCCGGCACGT CAAGGTGGAC 
ACACTGAGCC AGCCTGAGGC CCTCTCCAGG ATCTTGGTGC CAGCTGCTTG GTGCACGGTG 
GGACGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316678.1
CDS	225..1136
Translation	MMMGSCPWRNSSSSLQMASLMRKNWRISFTRLTLTTPNYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD

Target ORF information:

RefSeq Version	NM_001329749.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329749.2

ORF Insert Sequence:

ATGATGATGG GAAGCTGTCC TTGGAGGAAT TCCAGCTCTT CTTTGCAGAT GGCGTCCTTA 
ATGAGAAAGA ACTGGAGGAT CTCTTTCACA CGATTGACTC TGACAACACC AAATTACTTT 
GTGGACCACA TGGGTGACTA TGAGGATGTC CTGGCCTCCC TGGAGACCTT GAATCACTCT 
GTCCTGAAGG CCATGGGTTA TACCAAGAAG GTATATGAGG GTGGGAGCAA CGTGGACCAG 
TTTGTGACCC GCTTCCTCCT GAAGGAGACG GCCAATCAGA TCCAGTCGCT GCTGAGCTCA 
GTGGAGAGTG CGGTGGAGGC CATCGAGGAA CAGACCAGCC AGCTCCGACA GAACCACATC 
AAACCCAGCC ACAGCGCGGC ACAGACCTGG TGTGGAAGCC CCACTCCCGC CTCTGCCCCC 
AACCACAAGC TCATGGCTAT GGAACAAGGC AAGACCCTTC CATCTGCCAC GGAGGATGCA 
AAGGAAGAGG GTCTGGAAGC CCAGATCAGC CGCTTGGCAG AGCTGATTGG GAGGCTGGAG 
AGCAAAGCAC TGTGGTTCGA CCTGCAGCAG CGCCTGTCAG ATGAAGATGG CACCAACATG 
CACCTGCAGC TGGTCCGGCA GGAGATGGCC GTGTGCCCCG AGCAACTGAG CGAGTTTCTG 
GACTCTCTGC GCCAGTATCT GCGGGGGACC ACTGGCGTGA GGAACTGCTT CCACATCACT 
GCCGTGAGGC TCTCAGATGG CTTCACCTTT GTCATCTATG AGTTCTGGGA GACAGAGGAG 
GCGTGGAAGA GGCACCTGCA GAGCCCCCTG TGTAAGGCGT TCCGGCACGT CAAGGTGGAC 
ACACTGAGCC AGCCTGAGGC CCTCTCCAGG ATCTTGGTGC CAGCTGCTTG GTGCACGGTG 
GGACGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NECAB2 ( XM_017023317.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878806.1 Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu86085
Clone ID Related Accession (Same CDS sequence)	XM_017023317.1 , XM_017023317.2
Accession Version	XM_017023317.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTGG GCCTGCGTGT GTGTGTCAGC GTGGCTGGGA GCAGGCTGGT GGTACTGGCA 
GCCGCTTCCA CGTGGGTGAG GGGAGGGCCT CTGTCTGCAA CATCCCTCAT CAGTTCCCCC 
TGGATCCCAG CCCGGGGAGG GGGAGAGGGA AGGGGGGCTC AGCTTCCCAG GACCCTAAGC 
TGCTCCTGGG AGAAGAGAAG GATCCCAGTA TCCGGTCAGA GGCTGCAGGA AGCGCTTGCC 
TCTCCCTGGC CCAGGGCTGA CTCCGCCTCT CTTTTCTGCA GATTTTCCGC CGTGCGGACA 
AAAATGATGA TGGGAAGCTG TCCTTGGAGG AATTCCAGCT CTTCTTTGCA GATGGCGTCC 
TTAATGAGAA AGAACTGGAG GATCTCTTTC ACACGATTGA CTCTGACAAC ACCAAATTAC 
TTTGTGGACC ACATGGGTGA CTATGAGGAT GTCCTGGCCT CCCTGGAGAC CTTGAATCAC 
TCTGTCCTGA AGGCCATGGG TTATACCAAG AAGGTATATG AGGGTGGGAG CAACGTGGAC 
CAGTTTGTGA CCCGCTTCCT CCTGAAGGAG ACGGCCAATC AGATCCAGTC GCTGCTGAGC 
TCAGTGGAGA GTGCGGTGGA GGCCATCGAG GAACAGACCA GCCAGCTCCG ACAGAACCAC 
ATCAAACCCA GCCACAGCGC GGCACAGACC TGGTGTGGAA GCCCCACTCC CGCCTCTGCC 
CCCAACCACA AGCTCATGGC TATGGAACAA GGCAAGACCC TTCCATCTGC CACGGAGGAT 
GCAAAGGAAG AGGGTCTGGA AGCCCAGATC AGCCGCTTGG CAGAGCTGAT TGGGAGGCTG 
GAGAGCAAAG CACTGTGGTT CGACCTGCAG CAGCGCCTGT CAGATGAAGA TGGCACCAAC 
ATGCACCTGC AGCTGGTCCG GCAGGAGATG GCCGTGTGCC CCGAGCAACT GAGCGAGTTT 
CTGGACTCTC TGCGCCAGTA TCTGCGGGGG ACCACTGGCG TGAGGAACTG CTTCCACATC 
ACTGCCGTGA GGCTCTCAGA TGGCTTCACC TTTGTCATCT ATGAGTTCTG GGAGACAGAG 
GAGGCGTGGA AGAGGCACCT GCAGAGCCCC CTGTGTAAGG CGTTCCGGCA CGTCAAGGTG 
GACACACTGA GCCAGCCTGA GGCCCTCTCC AGGATCTTGG TGCCAGCTGC TTGGTGCACG 
GTGGGACGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878806.1
CDS	819..2033
Translation	MAVGLRVCVSVAGSRLVVLAAASTWVRGGPLSATSLISSPWIPARGGGEGRGAQLPRTLSCSWEKRRIPVSGQRLQEALASPWPRADSASLFCRFSAVRTKMMMGSCPWRNSSSSLQMASLMRKNWRISFTRLTLTTPNYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD

Target ORF information:

RefSeq Version	XM_017023317.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023317.1

ORF Insert Sequence:

ATGGCCGTGG GCCTGCGTGT GTGTGTCAGC GTGGCTGGGA GCAGGCTGGT GGTACTGGCA 
GCCGCTTCCA CGTGGGTGAG GGGAGGGCCT CTGTCTGCAA CATCCCTCAT CAGTTCCCCC 
TGGATCCCAG CCCGGGGAGG GGGAGAGGGA AGGGGGGCTC AGCTTCCCAG GACCCTAAGC 
TGCTCCTGGG AGAAGAGAAG GATCCCAGTA TCCGGTCAGA GGCTGCAGGA AGCGCTTGCC 
TCTCCCTGGC CCAGGGCTGA CTCCGCCTCT CTTTTCTGCA GATTTTCCGC CGTGCGGACA 
AAAATGATGA TGGGAAGCTG TCCTTGGAGG AATTCCAGCT CTTCTTTGCA GATGGCGTCC 
TTAATGAGAA AGAACTGGAG GATCTCTTTC ACACGATTGA CTCTGACAAC ACCAAATTAC 
TTTGTGGACC ACATGGGTGA CTATGAGGAT GTCCTGGCCT CCCTGGAGAC CTTGAATCAC 
TCTGTCCTGA AGGCCATGGG TTATACCAAG AAGGTATATG AGGGTGGGAG CAACGTGGAC 
CAGTTTGTGA CCCGCTTCCT CCTGAAGGAG ACGGCCAATC AGATCCAGTC GCTGCTGAGC 
TCAGTGGAGA GTGCGGTGGA GGCCATCGAG GAACAGACCA GCCAGCTCCG ACAGAACCAC 
ATCAAACCCA GCCACAGCGC GGCACAGACC TGGTGTGGAA GCCCCACTCC CGCCTCTGCC 
CCCAACCACA AGCTCATGGC TATGGAACAA GGCAAGACCC TTCCATCTGC CACGGAGGAT 
GCAAAGGAAG AGGGTCTGGA AGCCCAGATC AGCCGCTTGG CAGAGCTGAT TGGGAGGCTG 
GAGAGCAAAG CACTGTGGTT CGACCTGCAG CAGCGCCTGT CAGATGAAGA TGGCACCAAC 
ATGCACCTGC AGCTGGTCCG GCAGGAGATG GCCGTGTGCC CCGAGCAACT GAGCGAGTTT 
CTGGACTCTC TGCGCCAGTA TCTGCGGGGG ACCACTGGCG TGAGGAACTG CTTCCACATC 
ACTGCCGTGA GGCTCTCAGA TGGCTTCACC TTTGTCATCT ATGAGTTCTG GGAGACAGAG 
GAGGCGTGGA AGAGGCACCT GCAGAGCCCC CTGTGTAAGG CGTTCCGGCA CGTCAAGGTG 
GACACACTGA GCCAGCCTGA GGCCCTCTCC AGGATCTTGG TGCCAGCTGC TTGGTGCACG 
GTGGGACGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NECAB2 ( XM_017023317.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878806.1 Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA.

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CloneID	OHu86085
Clone ID Related Accession (Same CDS sequence)	XM_017023317.1 , XM_017023317.2
Accession Version	XM_017023317.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	N-terminal EF-hand calcium-binding protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023317.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTGG GCCTGCGTGT GTGTGTCAGC GTGGCTGGGA GCAGGCTGGT GGTACTGGCA 
GCCGCTTCCA CGTGGGTGAG GGGAGGGCCT CTGTCTGCAA CATCCCTCAT CAGTTCCCCC 
TGGATCCCAG CCCGGGGAGG GGGAGAGGGA AGGGGGGCTC AGCTTCCCAG GACCCTAAGC 
TGCTCCTGGG AGAAGAGAAG GATCCCAGTA TCCGGTCAGA GGCTGCAGGA AGCGCTTGCC 
TCTCCCTGGC CCAGGGCTGA CTCCGCCTCT CTTTTCTGCA GATTTTCCGC CGTGCGGACA 
AAAATGATGA TGGGAAGCTG TCCTTGGAGG AATTCCAGCT CTTCTTTGCA GATGGCGTCC 
TTAATGAGAA AGAACTGGAG GATCTCTTTC ACACGATTGA CTCTGACAAC ACCAAATTAC 
TTTGTGGACC ACATGGGTGA CTATGAGGAT GTCCTGGCCT CCCTGGAGAC CTTGAATCAC 
TCTGTCCTGA AGGCCATGGG TTATACCAAG AAGGTATATG AGGGTGGGAG CAACGTGGAC 
CAGTTTGTGA CCCGCTTCCT CCTGAAGGAG ACGGCCAATC AGATCCAGTC GCTGCTGAGC 
TCAGTGGAGA GTGCGGTGGA GGCCATCGAG GAACAGACCA GCCAGCTCCG ACAGAACCAC 
ATCAAACCCA GCCACAGCGC GGCACAGACC TGGTGTGGAA GCCCCACTCC CGCCTCTGCC 
CCCAACCACA AGCTCATGGC TATGGAACAA GGCAAGACCC TTCCATCTGC CACGGAGGAT 
GCAAAGGAAG AGGGTCTGGA AGCCCAGATC AGCCGCTTGG CAGAGCTGAT TGGGAGGCTG 
GAGAGCAAAG CACTGTGGTT CGACCTGCAG CAGCGCCTGT CAGATGAAGA TGGCACCAAC 
ATGCACCTGC AGCTGGTCCG GCAGGAGATG GCCGTGTGCC CCGAGCAACT GAGCGAGTTT 
CTGGACTCTC TGCGCCAGTA TCTGCGGGGG ACCACTGGCG TGAGGAACTG CTTCCACATC 
ACTGCCGTGA GGCTCTCAGA TGGCTTCACC TTTGTCATCT ATGAGTTCTG GGAGACAGAG 
GAGGCGTGGA AGAGGCACCT GCAGAGCCCC CTGTGTAAGG CGTTCCGGCA CGTCAAGGTG 
GACACACTGA GCCAGCCTGA GGCCCTCTCC AGGATCTTGG TGCCAGCTGC TTGGTGCACG 
GTGGGACGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878806.1
CDS	508..1722
Translation	MAVGLRVCVSVAGSRLVVLAAASTWVRGGPLSATSLISSPWIPARGGGEGRGAQLPRTLSCSWEKRRIPVSGQRLQEALASPWPRADSASLFCRFSAVRTKMMMGSCPWRNSSSSLQMASLMRKNWRISFTRLTLTTPNYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKALWFDLQQRLSDEDGTNMHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD

Target ORF information:

RefSeq Version	XM_017023317.2
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023317.2

ORF Insert Sequence:

ATGGCCGTGG GCCTGCGTGT GTGTGTCAGC GTGGCTGGGA GCAGGCTGGT GGTACTGGCA 
GCCGCTTCCA CGTGGGTGAG GGGAGGGCCT CTGTCTGCAA CATCCCTCAT CAGTTCCCCC 
TGGATCCCAG CCCGGGGAGG GGGAGAGGGA AGGGGGGCTC AGCTTCCCAG GACCCTAAGC 
TGCTCCTGGG AGAAGAGAAG GATCCCAGTA TCCGGTCAGA GGCTGCAGGA AGCGCTTGCC 
TCTCCCTGGC CCAGGGCTGA CTCCGCCTCT CTTTTCTGCA GATTTTCCGC CGTGCGGACA 
AAAATGATGA TGGGAAGCTG TCCTTGGAGG AATTCCAGCT CTTCTTTGCA GATGGCGTCC 
TTAATGAGAA AGAACTGGAG GATCTCTTTC ACACGATTGA CTCTGACAAC ACCAAATTAC 
TTTGTGGACC ACATGGGTGA CTATGAGGAT GTCCTGGCCT CCCTGGAGAC CTTGAATCAC 
TCTGTCCTGA AGGCCATGGG TTATACCAAG AAGGTATATG AGGGTGGGAG CAACGTGGAC 
CAGTTTGTGA CCCGCTTCCT CCTGAAGGAG ACGGCCAATC AGATCCAGTC GCTGCTGAGC 
TCAGTGGAGA GTGCGGTGGA GGCCATCGAG GAACAGACCA GCCAGCTCCG ACAGAACCAC 
ATCAAACCCA GCCACAGCGC GGCACAGACC TGGTGTGGAA GCCCCACTCC CGCCTCTGCC 
CCCAACCACA AGCTCATGGC TATGGAACAA GGCAAGACCC TTCCATCTGC CACGGAGGAT 
GCAAAGGAAG AGGGTCTGGA AGCCCAGATC AGCCGCTTGG CAGAGCTGAT TGGGAGGCTG 
GAGAGCAAAG CACTGTGGTT CGACCTGCAG CAGCGCCTGT CAGATGAAGA TGGCACCAAC 
ATGCACCTGC AGCTGGTCCG GCAGGAGATG GCCGTGTGCC CCGAGCAACT GAGCGAGTTT 
CTGGACTCTC TGCGCCAGTA TCTGCGGGGG ACCACTGGCG TGAGGAACTG CTTCCACATC 
ACTGCCGTGA GGCTCTCAGA TGGCTTCACC TTTGTCATCT ATGAGTTCTG GGAGACAGAG 
GAGGCGTGGA AGAGGCACCT GCAGAGCCCC CTGTGTAAGG CGTTCCGGCA CGTCAAGGTG 
GACACACTGA GCCAGCCTGA GGCCCTCTCC AGGATCTTGG TGCCAGCTGC TTGGTGCACG 
GTGGGACGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NECAB2 ( NM_001329748.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316677.1 Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 2, mRNA.

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CloneID	OHu101805
Clone ID Related Accession (Same CDS sequence)	NM_001329748.1
Accession Version	NM_001329748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	N-terminal EF-hand calcium-binding protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC040169.7. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' end compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGCGAGC GGGCGGCGCG CCTGTGCAGG GCCGGCGCGC ACAGGCTGCT CCGGGAGCCG 
CCGCAGCAGG GCCGGGCGCT GGGCGGGCTG CTGCGCTGGG TGGGCGCCAG GATGGGCGAG 
CCCCGGGAGT CGCTGGCCCC CGCCGCCCCC GCGGACCCCG GCCCAGCCTC GCCGCGCGGG 
GGCACCGCCG TCATCCTGGA CATTTTCCGC CGTGCGGACA AAAATGATGA TGGGAAGCTG 
TCCTTGGAGG AATTCCAGCT CTTCTTTGCA GATGGCGTCC TTAATGAGAA AGAACTGGAG 
GATCTCTTTC ACACGATTGA CTCTGACAAC ACCAACCATG TGGACACCAA GGAGCTGTGT 
GATTACTTTG TGGACCACAT GGGTGACTAT GAGGATGTCC TGGCCTCCCT GGAGACCTTG 
AATCACTCTG TCCTGAAGGC CATGGGTTAT ACCAAGAAGG TATATGAGGG TGGGAGCAAC 
GTGGACCAGT TTGTGACCCG CTTCCTCCTG AAGGAGACGG CCAATCAGAT CCAGTCGCTG 
CTGAGCTCAG TGGAGAGTGC GGTGGAGGCC ATCGAGGAAC AGACCAGCCA GCTCCGACAG 
AACCACATCA AACCCAGCCA CAGCGCGGCA CAGACCTGGT GTGGAAGCCC CACTCCCGCC 
TCTGCCCCCA ACCACAAGCT CATGGCTATG GAACAAGGCA AGACCCTTCC ATCTGCCACG 
GAGGATGCAA AGGAAGAGGG TCTGGAAGCC CAGATCAGCC GCTTGGCAGA GCTGATTGGG 
AGGCTGGAGA GCAAACACCT GCAGCTGGTC CGGCAGGAGA TGGCCGTGTG CCCCGAGCAA 
CTGAGCGAGT TTCTGGACTC TCTGCGCCAG TATCTGCGGG GGACCACTGG CGTGAGGAAC 
TGCTTCCACA TCACTGCCGT GAGGCTCTCA GATGGCTTCA CCTTTGTCAT CTATGAGTTC 
TGGGAGACAG AGGAGGCGTG GAAGAGGCAC CTGCAGAGCC CCCTGTGTAA GGCGTTCCGG 
CACGTCAAGG TGGACACACT GAGCCAGCCT GAGGCCCTCT CCAGGATCTT GGTGCCAGCT 
GCTTGGTGCA CGGTGGGACG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316677.1
CDS	18..1124
Translation	MCERAARLCRAGAHRLLREPPQQGRALGGLLRWVGARMGEPRESLAPAAPADPGPASPRGGTAVILDIFRRADKNDDGKLSLEEFQLFFADGVLNEKELEDLFHTIDSDNTNHVDTKELCDYFVDHMGDYEDVLASLETLNHSVLKAMGYTKKVYEGGSNVDQFVTRFLLKETANQIQSLLSSVESAVEAIEEQTSQLRQNHIKPSHSAAQTWCGSPTPASAPNHKLMAMEQGKTLPSATEDAKEEGLEAQISRLAELIGRLESKHLQLVRQEMAVCPEQLSEFLDSLRQYLRGTTGVRNCFHITAVRLSDGFTFVIYEFWETEEAWKRHLQSPLCKAFRHVKVDTLSQPEALSRILVPAAWCTVGRD

Target ORF information:

RefSeq Version	NM_001329748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens N-terminal EF-hand calcium binding protein 2 (NECAB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329748.1

ORF Insert Sequence:

ATGTGCGAGC GGGCGGCGCG CCTGTGCAGG GCCGGCGCGC ACAGGCTGCT CCGGGAGCCG 
CCGCAGCAGG GCCGGGCGCT GGGCGGGCTG CTGCGCTGGG TGGGCGCCAG GATGGGCGAG 
CCCCGGGAGT CGCTGGCCCC CGCCGCCCCC GCGGACCCCG GCCCAGCCTC GCCGCGCGGG 
GGCACCGCCG TCATCCTGGA CATTTTCCGC CGTGCGGACA AAAATGATGA TGGGAAGCTG 
TCCTTGGAGG AATTCCAGCT CTTCTTTGCA GATGGCGTCC TTAATGAGAA AGAACTGGAG 
GATCTCTTTC ACACGATTGA CTCTGACAAC ACCAACCATG TGGACACCAA GGAGCTGTGT 
GATTACTTTG TGGACCACAT GGGTGACTAT GAGGATGTCC TGGCCTCCCT GGAGACCTTG 
AATCACTCTG TCCTGAAGGC CATGGGTTAT ACCAAGAAGG TATATGAGGG TGGGAGCAAC 
GTGGACCAGT TTGTGACCCG CTTCCTCCTG AAGGAGACGG CCAATCAGAT CCAGTCGCTG 
CTGAGCTCAG TGGAGAGTGC GGTGGAGGCC ATCGAGGAAC AGACCAGCCA GCTCCGACAG 
AACCACATCA AACCCAGCCA CAGCGCGGCA CAGACCTGGT GTGGAAGCCC CACTCCCGCC 
TCTGCCCCCA ACCACAAGCT CATGGCTATG GAACAAGGCA AGACCCTTCC ATCTGCCACG 
GAGGATGCAA AGGAAGAGGG TCTGGAAGCC CAGATCAGCC GCTTGGCAGA GCTGATTGGG 
AGGCTGGAGA GCAAACACCT GCAGCTGGTC CGGCAGGAGA TGGCCGTGTG CCCCGAGCAA 
CTGAGCGAGT TTCTGGACTC TCTGCGCCAG TATCTGCGGG GGACCACTGG CGTGAGGAAC 
TGCTTCCACA TCACTGCCGT GAGGCTCTCA GATGGCTTCA CCTTTGTCAT CTATGAGTTC 
TGGGAGACAG AGGAGGCGTG GAAGAGGCAC CTGCAGAGCC CCCTGTGTAA GGCGTTCCGG 
CACGTCAAGG TGGACACACT GAGCCAGCCT GAGGCCCTCT CCAGGATCTT GGTGCCAGCT 
GCTTGGTGCA CGGTGGGACG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative anatomical distribution of neuronal calcium-binding protein (NECAB) 1 and -2 in rodent and human spinal cord.
Brain structure & function221(7)3803-23(2016 09)
Zhang MD,Barde S,Szodorai E,Josephson A,Mitsios N,Watanabe M,Attems J,Lubec G,Kov?cs GG,Uhl?n M,Mulder J,Harkany T,H?kfelt T

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

The association of metabotropic glutamate receptor type 5 with the neuronal Ca2+-binding protein 2 modulates receptor function.
Journal of neurochemistry111(2)555-67(2009 Oct)
Canela L,Fern?ndez-Due?as V,Albergaria C,Watanabe M,Llu?s C,Mallol J,Canela EI,Franco R,Luj?n R,Ciruela F

The neuronal Ca(2+) -binding protein 2 (NECAB2) interacts with the adenosine A(2A) receptor and modulates the cell surface expression and function of the receptor.
Molecular and cellular neurosciences36(1)1-12(2007 Sep)
Canela L,Luj?n R,Llu?s C,Burgue?o J,Mallol J,Canela EI,Franco R,Ciruela F

NECABs: a family of neuronal Ca(2+)-binding proteins with an unusual domain structure and a restricted expression pattern.
Neuroscience112(1)51-63(2002)
Sugita S,Ho A,S?dhof TC

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NECAB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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