MIEF1 cDNA ORF clone, Homo sapiens(human)

The following MIEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25060 NM_019008.5
Latest version!
Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu25060 XM_011530236.2
Latest version!
Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu25060 XM_011530238.2
Latest version!
Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu25060 XM_017028838.2
Latest version!
Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu25060 XM_011530237.2
Latest version!
Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu68356 NM_001304564.1
Latest version!
Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu25060 XM_017028838.1 Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu25060 XM_011530237.1 Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu25060 XM_011530236.1 Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu25060 XM_011530238.1 Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version NM_019008.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA192893.1, AL365515.1, BC047292.1 and BC054037.1. On Feb 4, 2015 this sequence version replaced NM_019008.4. Transcript Variant: This variant (1) represents a predominant transcript, which encodes a 463aa protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.52664.1, SRR1803612.83269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 21508961; reported by MitoCarta RefSeq Select criteria :: based on conservation, expression regulatory uORF :: PMID: 25621764 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_061881.2
    CDS548..1939
    Translation

    Target ORF information:

    RefSeq Version NM_019008.5
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_019008.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version XM_011530237.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528539.1
    CDS736..2127
    Translation

    Target ORF information:

    RefSeq Version XM_011530237.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530237.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version XM_011530238.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528540.1
    CDS820..2211
    Translation

    Target ORF information:

    RefSeq Version XM_011530238.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530238.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version XM_017028838.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884327.1
    CDS649..2040
    Translation

    Target ORF information:

    RefSeq Version XM_017028838.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028838.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version XM_017028838.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884327.1
    CDS714..2105
    Translation

    Target ORF information:

    RefSeq Version XM_017028838.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017028838.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version XM_011530237.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528539.1
    CDS593..1984
    Translation

    Target ORF information:

    RefSeq Version XM_011530237.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530237.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version XM_011530236.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528538.1
    CDS616..2007
    Translation

    Target ORF information:

    RefSeq Version XM_011530236.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530236.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version XM_011530236.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528538.1
    CDS616..2007
    Translation

    Target ORF information:

    RefSeq Version XM_011530236.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530236.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25060
    Clone ID Related Accession (Same CDS sequence) XM_011530237.1 , XM_011530238.1 , XM_017028838.2 , XM_017028838.1 , XM_011530237.2 , NM_019008.5 , XM_011530236.1 , XM_011530236.2 , XM_011530238.2
    Accession Version XM_011530238.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530238.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528540.1
    CDS756..2147
    Translation

    Target ORF information:

    RefSeq Version XM_011530238.2
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530238.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGTGAACT TATTTGCAGA GCTCACCCCT
    GAAGAAATAG ACGAATTAGG ATACACTCTG TATTGCTCAT TGTCTGAGCC AGAGGTGCTG
    CTGCAGACGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu68356
    Clone ID Related Accession (Same CDS sequence) NM_001304564.1
    Accession Version NM_001304564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-07
    Organism Homo sapiens(human)
    Product mitochondrial dynamics protein MID51 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL136768.1, BC002587.2, AL834205.1, BC047292.1 and BC054037.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon and lacks an internal segment in the 3' region, compared to variant 1. The resulting isoform (2) has a longer and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136768.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 21508961; reported by MitoCarta ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGACAAGG CCTTGAAGGA ACGCAGCCTT
    AGACATCAGG TGAGGATGAT GGAGGTAGAC AGTCGACTGA ATGTCAGCTG GAAAATCCAG
    TCACTAGTTG GGGTTTGGTG GCCATGTTTT CTACCCAGAC AGGCCCTGCT TTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291493.1
    CDS498..1934
    Translation

    Target ORF information:

    RefSeq Version NM_001304564.1
    Organism Homo sapiens(human)
    Definition Homo sapiens mitochondrial elongation factor 1 (MIEF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGCAGGCG CTGGTGAGCG CAAAGGCAAG AAGGATGACA ATGGCATTGG CACGGCCATT 
    GACTTTGTGC TCTCCAATGC CCGGCTGGTG CTGGGGGTGG GTGGAGCGGC CATGCTGGGC
    ATCGCCACGC TGGCAGTTAA GCGGATGTAC GATCGGGCGA TCAGTGCCCC TACCAGCCCC
    ACCCGCCTGA GCCATTCGGG GAAAAGGAGC TGGGAAGAAC CCAACTGGAT GGGCTCCCCA
    CGACTGCTGA ACAGGGACAT GAAGACGGGC CTGAGCCGGT CCTTGCAGAC CCTTCCCACA
    GACTCCTCCA CCTTCGACAC AGATACATTC TGCCCGCCCC GGCCCAAGCC AGTGGCCAGG
    AAGGGCCAGG TAGACTTGAA GAAGTCACGA CTCCGCATGT CCCTGCAGGA GAAACTTCTT
    ACTTACTACC GGAACCGGGC AGCCATCCCT GCTGGAGAGC AGGCTCGGGC CAAGCAAGCT
    GCTGTGGACA TATGTGCCGA GCTCCGGAGC TTCCTGCGGG CCAAGTTGCC TGACATGCCG
    CTTCGGGACA TGTACTTGAG TGGCAGCCTC TACGATGACC TGCAGGTGGT GACAGCTGAC
    CACATCCAAC TCATTGTGCC CCTTGTGCTG GAGCAGAACC TGTGGTCATG TATTCCTGGT
    GAAGACACCA TCATGAATGT CCCTGGCTTC TTCCTGGTGC GTCGTGAGAA TCCAGAGTAC
    TTTCCTCGTG GGAGCAGTTA CTGGGACCGC TGTGTAGTAG GGGGCTACCT CTCTCCAAAG
    ACAGTCGCAG ATACATTTGA GAAGGTAGTG GCTGGCTCCA TCAATTGGCC AGCCATAGGG
    TCCCTCTTGG ACTATGTGAT CCGCCCGGCC CCACCCCCAG AAGCCCTCAC ACTGGAGGTG
    CAGTATGAGC GTGACAAACA TCTCTTCATT GACTTCCTGC CATCAGTGAC CCTCGGTGAC
    ACAGTCTTGG TGGCCAAACC ACACCGGCTA GCCCAGTATG ACAACCTGTG GCGGCTGAGC
    CTGCGTCCCG CGGAGACGGC ACGCCTGCGG GCTCTGGACC AGGCTGACTC GGGCTGCCGA
    TCTCTGTGCC TCAAGATCCT CAAGGCCATA TGCAAGTCCA CCCCGGCTCT GGGCCACCTC
    ACTGCCAGCC AGCTAACCAA TGTCATCCTC CACTTGGCCC AGGAGGAGGC TGACTGGTCT
    CCGGATATGC TGGCCGACCG TTTCCTGCAG GCCTTGAGGG GACTTATCAG CTACTTAGAG
    GCTGGAGTCC TGCCCAGTGC CCTAAACCCC AAGGACAAGG CCTTGAAGGA ACGCAGCCTT
    AGACATCAGG TGAGGATGAT GGAGGTAGAC AGTCGACTGA ATGTCAGCTG GAAAATCCAG
    TCACTAGTTG GGGTTTGGTG GCCATGTTTT CTACCCAGAC AGGCCCTGCT TTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.