PLN cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PLN
Entrez Gene ID	5350
Full Name	phospholamban
Synonyms	CMD1P,CMH18,PLB
General protein information	Preferred Names phospholamban Names cardiac phospholamban
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q22.31
Summary	The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016].
Disorder MIM:	172405
Disorder Html:	Cardiomyopathy, dilated, 1P, 609909 (3); Cardiomyopathy, hypertrophic, 18, 613874 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002667.4	NP_002658.1	cardiac phospholamban
NM_002667.3	NP_002658.1	cardiac phospholamban
NM_002667.5	NP_002658.1	cardiac phospholamban

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PLN	XP_003311500.1
Homo sapiens (human)	PLN	NP_002658.1

Gene Ontology
Bibliography

Related articles in PubMed

Reversal of Phospholamban Inhibition of the Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA) Using Short, Protein-interacting RNAs and Oligonucleotide Analogs.
Soller KJ, Yang J, Veglia G, Bowser MT
The Journal of biological chemistry291(41)21510-21518(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

microRNAs (miRNAs) 1 and 21 bind PLN strongly and relieve PLN inhibition of SERCA to a greater extent than a similar length random sequence RNA mixture.
Title: Reversal of Phospholamban Inhibition of the Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA) Using Short, Protein-interacting RNAs and Oligonucleotide Analogs.

LMOD1, SYNPO2, PDLIM7, PLN, and SYNM down-regulation reflect the altered phenotype of smooth muscle cells in vascular disease and could be early sensitive markers of SMC dedifferentiation.
Title: Phenotypic Modulation of Smooth Muscle Cells in Atherosclerosis Is Associated With Downregulation of LMOD1, SYNPO2, PDLIM7, PLN, and SYNM.

These data suggest that PLN is, at least partially, oligo-ubiquitinated at Lys(3) and degraded through Ser(16)-phosphorylation-mediated poly-ubiquitination during heart failure.
Title: Phospholamban degradation is induced by phosphorylation-mediated ubiquitination and inhibited by interaction with cardiac type Sarco(endo)plasmic reticulum Ca(2+)-ATPase.

Data suggest phospholamban (PLN) gene is a rare cause of cardiomyopathy in African patients.
Title: Mutation analysis of the phospholamban gene in 315 South Africans with dilated, hypertrophic, peripartum and arrhythmogenic right ventricular cardiomyopathies.

Phospholamban and sarcolipin are membrane proteins that differentially regulate SERCA function. (Review)
Title: Phospholamban and sarcolipin: Are they functionally redundant or distinct regulators of the Sarco(Endo)Plasmic Reticulum Calcium ATPase?

The following PLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17925	NM_002667.5 Latest version!	Homo sapiens phospholamban (PLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu17925	NM_002667.3	Homo sapiens phospholamban (PLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17925	NM_002667.4	Homo sapiens phospholamban (PLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PLN ( NM_002667.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002658.1 Homo sapiens phospholamban (PLN), mRNA.

Price & Availability↑

CloneID	OHu17925
Clone ID Related Accession (Same CDS sequence)	NM_002667.4 , NM_002667.3 , NM_002667.5
Accession Version	NM_002667.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	cardiac phospholamban
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP257094.1, M63603.1, BC005269.1, AK129844.1 and N80934.1. On Apr 25, 2016 this sequence version replaced NM_002667.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP257094.1, SRR1803616.1555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA 
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002658.1
CDS	228..386
Translation	MEKVQYLTRSAIRRASTIEMPQQARQKLQNLFINFCLILICLLLICIIVMLL

Target ORF information:

RefSeq Version	NM_002667.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholamban (PLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002667.4

ORF Insert Sequence:

1
61
121

ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA 
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLN ( NM_002667.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002658.1 Homo sapiens phospholamban (PLN), mRNA.

Price & Availability↑

CloneID	OHu17925
Clone ID Related Accession (Same CDS sequence)	NM_002667.4 , NM_002667.3 , NM_002667.5
Accession Version	NM_002667.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	cardiac phospholamban
Comment

1
61
121

ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA 
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002658.1
Translation	MEKVQYLTRSAIRRASTIEMPQQARQKLQNLFINFCLILICLLLICIIVMLL

Target ORF information:

RefSeq Version	NM_002667.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens phospholamban (PLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002667.3

ORF Insert Sequence:

1
61
121

ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA 
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PLN ( NM_002667.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002658.1 Homo sapiens phospholamban (PLN), mRNA.

Price & Availability↑

CloneID	OHu17925
Clone ID Related Accession (Same CDS sequence)	NM_002667.4 , NM_002667.3 , NM_002667.5
Accession Version	NM_002667.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	cardiac phospholamban
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP257094.1, M63603.1, BC005269.1, AK129844.1, N80934.1 and Z99496.1. On Nov 22, 2018 this sequence version replaced NM_002667.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.217116.1, SRR1660809.23734.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000357525.6/ ENSP00000350132.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121

ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA 
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002658.1
CDS	195..353
Translation	MEKVQYLTRSAIRRASTIEMPQQARQKLQNLFINFCLILICLLLICIIVMLL

Target ORF information:

RefSeq Version	NM_002667.5
Organism	Homo sapiens(human)
Definition	Homo sapiens phospholamban (PLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002667.5

ORF Insert Sequence:

1
61
121

ATGGAGAAAG TCCAATACCT CACTCGCTCA GCTATAAGAA GAGCCTCAAC CATTGAAATG 
CCTCAACAAG CACGTCAAAA GCTACAGAAT CTATTTATCA ATTTCTGTCT CATCTTAATA 
TGTCTCTTGC TGATCTGTAT CATCGTGATG CTTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Reversal of Phospholamban Inhibition of the Sarco(endo)plasmic Reticulum Ca2+-ATPase (SERCA) Using Short, Protein-interacting RNAs and Oligonucleotide Analogs.
The Journal of biological chemistry291(41)21510-21518(2016 Oct)
Soller KJ,Yang J,Veglia G,Bowser MT

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PLN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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