ATP5F1E cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP5F1E
Entrez Gene ID	514
Full Name	ATP synthase F1 subunit epsilon
Synonyms	ATP5E,ATPE,MC5DN3
General protein information	Preferred Names ATP synthase F1 subunit epsilon Names ATP synthase subunit epsilon, mitochondrial ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit F(0)F(1)-ATPase H(+)-transporting two-sector ATPase mitochondrial ATP synthase epsilon chain mitochondrial ATPase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.32
Summary	This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the epsilon subunit of the catalytic core. Two pseudogenes of this gene are located on chromosomes 4 and 13. Read-through transcripts that include exons from this gene are expressed from the upstream gene SLMO2.[provided by RefSeq, Mar 2011].
Disorder MIM:	606153
Disorder Html:	?Mitochondrial complex V (ATP synthase) deficiency, nuclear type 3, 614053 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006886.3	NP_008817.1	ATP synthase subunit epsilon, mitochondrial
NM_006886.4	NP_008817.1	ATP synthase subunit epsilon, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP5E	NP_008817.1
	ATP5E	NP_001028073.1
Pan troglodytes (chimpanzee)	ATP5E	XP_003317076.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Molecular Analysis by Gene Expression of Mitochondrial ATPase Subunits in Papillary Thyroid Cancer: Is ATP5E Transcript a Possible Early Tumor Marker?
Hurtado-L?pez LM, Fern?ndez-Ram?rez F, Mart?nez-Pe?afiel E, Carrillo Ruiz JD, Herrera Gonz?lez NE
Medical science monitor : international medical journal of experimental and clinical research211745-51(2015 Jun)

Cell surface F1/FO ATP synthase contributes to interstitial flow-mediated development of the acidic microenvironment in tumor tissues.
Kawai Y, Kaidoh M, Yokoyama Y, Ohhashi T
American journal of physiology. Cell physiology305(11)C1139-50(2013 Dec)

Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit.
Mayr JA, Havl?ckov? V, Zimmermann F, Magler I, Kaplanov? V, Jesina P, Pecinov? A, Nuskov? H, Koch J, Sperl W, Houstek J
Human molecular genetics19(17)3430-9(2010 Sep)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ATP5E transcript alteration (down-expression) was highly associated to PTC diagnosis
Title: Molecular Analysis by Gene Expression of Mitochondrial ATPase Subunits in Papillary Thyroid Cancer: Is ATP5E Transcript a Possible Early Tumor Marker?

The cell surface F1/F0 ATP synthase complex plays a key role in the acidic microenvironment of tumor tissues.
Title: Cell surface F1/FO ATP synthase contributes to interstitial flow-mediated development of the acidic microenvironment in tumor tissues.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

the epsilon subunit is essential for the assembly of F1 and plays an important role in the incorporation of the hydrophobic subunit c into the F1-c oligomer rotor of the mitochondrial ATP synthase complex
Title: Knockdown of F1 epsilon subunit decreases mitochondrial content of ATP synthase and leads to accumulation of subunit c.

Galectin 3 co-localized with ATP synthase in the inner membrane of mitochondria and had an inhibitory effect on ATP synthase in human colon cancer cells.
Title: Identification of mitochondrial F(1)F(0)-ATP synthase interacting with galectin-3 in colon cancer cells.

The following ATP5F1E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP5F1E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19249	NM_006886.4 Latest version!	Homo sapiens ATP synthase F1 subunit epsilon (ATP5F1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu19249	NM_006886.3	Homo sapiens ATP synthase F1 subunit epsilon (ATP5F1E), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP5F1E ( NM_006886.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_008817.1 Homo sapiens ATP synthase F1 subunit epsilon (ATP5F1E), mRNA.

Price & Availability↑

CloneID	OHu19249
Clone ID Related Accession (Same CDS sequence)	NM_006886.3 , NM_006886.4
Accession Version	NM_006886.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-23
Organism	Homo sapiens(human)
Product	ATP synthase subunit epsilon, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB106920.1 and DA202623.1. This sequence is a reference standard in the RefSeqGene project. On or before Sep 17, 2011 this sequence version replaced NM_001001977.1, NM_006886.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD385581.1, CB106920.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

1
61
121

ATGGTGGCCT ACTGGAGACA GGCTGGACTC AGCTACATCC GATACTCCCA GATCTGTGCA 
AAAGCAGTGA GAGATGCACT GAAGACAGAA TTCAAAGCAA ATGCTGAGAA GACTTCTGGC 
AGCAACGTAA AAATTGTGAA AGTAAAGAAG GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008817.1
CDS	117..272
Translation	MVAYWRQAGLSYIRYSQICAKAVRDALKTEFKANAEKTSGSNVKIVKVKKE

Target ORF information:

RefSeq Version	NM_006886.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase F1 subunit epsilon (ATP5F1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006886.3

ORF Insert Sequence:

1
61
121

ATGGTGGCCT ACTGGAGACA GGCTGGACTC AGCTACATCC GATACTCCCA GATCTGTGCA 
AAAGCAGTGA GAGATGCACT GAAGACAGAA TTCAAAGCAA ATGCTGAGAA GACTTCTGGC 
AGCAACGTAA AAATTGTGAA AGTAAAGAAG GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP5F1E ( NM_006886.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_008817.1 Homo sapiens ATP synthase F1 subunit epsilon (ATP5F1E), mRNA.

Price & Availability↑

CloneID	OHu19249
Clone ID Related Accession (Same CDS sequence)	NM_006886.3 , NM_006886.4
Accession Version	NM_006886.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	ATP synthase subunit epsilon, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB106920.1, DA202623.1, AL109840.24 and KF495837.1. On Nov 22, 2018 this sequence version replaced NM_006886.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.195812.1, SRR1803612.248998.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000243997.8/ ENSP00000243997.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121

ATGGTGGCCT ACTGGAGACA GGCTGGACTC AGCTACATCC GATACTCCCA GATCTGTGCA 
AAAGCAGTGA GAGATGCACT GAAGACAGAA TTCAAAGCAA ATGCTGAGAA GACTTCTGGC 
AGCAACGTAA AAATTGTGAA AGTAAAGAAG GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008817.1
CDS	85..240
Translation	MVAYWRQAGLSYIRYSQICAKAVRDALKTEFKANAEKTSGSNVKIVKVKKE

Target ORF information:

RefSeq Version	NM_006886.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ATP synthase F1 subunit epsilon (ATP5F1E), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006886.4

ORF Insert Sequence:

1
61
121

ATGGTGGCCT ACTGGAGACA GGCTGGACTC AGCTACATCC GATACTCCCA GATCTGTGCA 
AAAGCAGTGA GAGATGCACT GAAGACAGAA TTCAAAGCAA ATGCTGAGAA GACTTCTGGC 
AGCAACGTAA AAATTGTGAA AGTAAAGAAG GAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Molecular Analysis by Gene Expression of Mitochondrial ATPase Subunits in Papillary Thyroid Cancer: Is ATP5E Transcript a Possible Early Tumor Marker?
Medical science monitor : international medical journal of experimental and clinical research211745-51(2015 Jun)
Hurtado-L?pez LM,Fern?ndez-Ram?rez F,Mart?nez-Pe?afiel E,Carrillo Ruiz JD,Herrera Gonz?lez NE

Cell surface F1/FO ATP synthase contributes to interstitial flow-mediated development of the acidic microenvironment in tumor tissues.
American journal of physiology. Cell physiology305(11)C1139-50(2013 Dec)
Kawai Y,Kaidoh M,Yokoyama Y,Ohhashi T

Mitochondrial ATP synthase deficiency due to a mutation in the ATP5E gene for the F1 epsilon subunit.
Human molecular genetics19(17)3430-9(2010 Sep)
Mayr JA,Havl?ckov? V,Zimmermann F,Magler I,Kaplanov? V,Jesina P,Pecinov? A,Nuskov? H,Koch J,Sperl W,Houstek J

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

ATP5F1E cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報